CN103141443B - A kind of regulate and control the method for beet webworm Ah lattice ichneumon wasp diapause - Google Patents
A kind of regulate and control the method for beet webworm Ah lattice ichneumon wasp diapause Download PDFInfo
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Abstract
The invention discloses a kind of regulate and control the method for beet webworm Ah lattice ichneumon wasp diapause.The method comprises the steps: 1) the beet webworm Ah lattice ichneumon wasp of pending Diapause inducement is placed in the condition of temperature 17 ± 1 DEG C, photoperiod L8:D16 to L12:D12 under induce, make described beet webworm Ah lattice ichneumon wasp enter diapause state; 2) the described beet webworm Ah lattice ichneumon wasp entering diapause state step 1) obtained stores under proceeding to temperature 4 DEG C, dark condition; 3) step 2) storage terminate after, under described beet webworm Ah lattice ichneumon wasp is proceeded to the condition of temperature 23 DEG C, photoperiod L14:D10 to L16:D8, recover grow.The method Diapause inducement rate 100%, the storage period after diapause can reach 120 days, within after Diapause termination 20 days, can obtain the one-tenth honeybee sprouted wings.The present invention is to the vast propagation of beet webworm Ah lattice ichneumon wasp, extend its product shelf phase and the prevention and control action time in field, improve control of insect efficiency, deepen the understanding to natural enemy insect developmental mechanism, explore insect all significant to the adaptation mechanism of environment and evolutionary path etc.
Description
Technical field
The invention belongs to biological technical field, relate to a kind of regulate and control the method for beet webworm Ah lattice ichneumon wasp diapause.
Background technology
In Grassland ecosystems, biocoenosis exists with multiple biotic population form, links together between them with the relation of food chain, and wherein any ring changes, and will inevitably cause the change of other links.Therefore, within the scope of certain Grassland ecosystems, analyze the population of insect and the correlation of natural enemy populations, strengthened the strength of natural enemy populations Control pests population artificially, just pest population quantity can be forced down the level that can not work the mischief to herbage.Natural enemy insect pest control is utilized to be methods most widely used, maximum in biological control.
Beet webworm Loxostege sticticalis Linnaeus(Lepidoptera: Pyralidae) be that the sudden many foods of farming and animal husbandry migrate insect, damaging range is wide, feeding habits are mixed, larva can take food 300 various plants such as Chenopodiaceae, polygonaceae, pulse family, Solanaceae, Curcurbitaceae, Cruciferae, very large to China North China, northeast and the harm of the Northwest's farming and animal husbandry, and often population outbreak, cause heavy economic losses.What the domestic control for beet webworm was mainly taked is chemical control, spray appropriate organic synthesis insecticide, the effect eliminated fast and reduce pest population density can be played, but excessively use chemical agent, have a strong impact on the quality of crops, and cause environmental pollution and residue of pesticide, harm human and livestock health.Therefore, change the task of top priority that the current beet webworm prevention and controls based on chemical control has become Development of Forage Grass Industry, the Pest control efficacy making full use of the abiogenous natural enemy insect in field is one of these important measures wherein, and tool is of great significance.
Beet webworm Ah lattice ichneumon wasp Agrypon flexorius Thunberg is subordinate to Hymenoptera, Ichneumonidae, Ah lattice's Uchida From China, it is holometabola parasitic wasp, total ovum, larva, pupa and these four worm states (developmental stage) of adult in growth course, the development characteristics of each worm state is had nothing in common with each other as ovum, pupa are not eaten motionless; Larva can only take food in pin main body, and mobility is very poor; Adult then has a wing, can circle in the air flexibly.Beet webworm Ah lattice ichneumon wasp is distributed in the areas such as Hebei, the Inner Mongol, Shanxi, is that farming and animal husbandry is migrated the advantage parasitic wasp of Loxostege stieticatis larva.Investigation finds that beet webworm Ah lattice ichneumon wasp is beet webworm parasitized larvae at advanced age natural enemy, its population quantity and dominance index are all higher, population dynamics tendency is also similar with the population dynamics tendency of beet webworm, there is obvious following phenomenon, be the advantage honeybee of local parasitic beet webworm, control beet webworm broken out to playing an important role.In order to develop the nature enemy in amount of this preciousness, the approach that artificial propagation discharges must be adopted.But adopt tradition to connect the method for generation breeding, easily degenerating in honeybee source, and not easily preserves for a long time, brings very large difficulty to field adaptability; By improving propagation technique, this honeybee of human-induced enters diapause status, storage life can be made greatly to extend, there is no diapause control technique, often cause natural enemy insect before insect occurs with regard to dead, or insect when occurring natural enemy insect product also do not produce, this is the bottleneck factor of serious restriction natural enemy insect industrialization production and large-area applications in field of biological control at present exactly.Therefore utilize cryopreservation diapause worm cocoon technology, control natural enemy development time, this is of crucial importance for expanding numerous natural enemy insect meaning the anniversary.
Summary of the invention
An object of the present invention is to provide a kind of regulate and control the method for beet webworm Ah lattice ichneumon wasp diapause.
The method of regulation and control beet webworm Ah lattice ichneumon wasp provided by the present invention diapause, specifically can comprise the steps:
(1) induce under the beet webworm Ah lattice ichneumon wasp of pending Diapause inducement being placed in the condition of temperature 17 ± 1 DEG C, photoperiod L8:D16 to L12:D12, make described beet webworm Ah lattice ichneumon wasp enter diapause state;
(2) the described beet webworm Ah lattice ichneumon wasp entering diapause state step (1) obtained stores under proceeding to temperature 4 DEG C, dark condition;
(3), after step (2) storage terminates, under described beet webworm Ah lattice ichneumon wasp is proceeded to the condition of temperature 23 DEG C, photoperiod L14:D10 to L16:D8, recover to grow.
Another object of the present invention is to provide a kind of beet webworm Ah lattice ichneumon wasp Diapause inducement and storage procedures.
Beet webworm Ah lattice ichneumon wasp Diapause inducement provided by the present invention and storage procedures, specifically can comprise the steps:
(1) induce under beet webworm Ah lattice ichneumon wasp being placed in the condition of temperature 17 ± 1 DEG C, photoperiod L8:D16 to L12:D12, make described beet webworm Ah lattice ichneumon wasp enter diapause state;
(2) the described beet webworm Ah lattice ichneumon wasp entering diapause state step (1) obtained stores under proceeding to temperature 4 DEG C, dark condition.
An also object of the present invention is to provide a kind of beet webworm Ah lattice ichneumon wasp Diapause inducement method.
Beet webworm Ah lattice ichneumon wasp Diapause inducement method provided by the present invention, induce under specifically can comprising the steps: that beet webworm Ah lattice ichneumon wasp is placed in the condition of temperature 17 ± 1 DEG C, photoperiod L8:D16 to L12:D12, make described beet webworm Ah lattice ichneumon wasp enter diapause state.
For three kinds of methods that the invention described above provides, in one embodiment of the invention, in step (1), described temperature is specially 17 DEG C; The described photoperiod is specially L8:D16, L10:D14 or L12:D12.
In one embodiment of the invention, in step (1), the intensity of illumination in described condition is 4000 ~ 5000LX; Relative air humidity in described condition is 70%-80%.
In three kinds of methods that the invention described above provides, the life stages sensitive that the beet webworm Ah lattice ichneumon wasp of described pending Diapause inducement experiences diapause signal is ovum or 1 instar larvae.
When the beet webworm Ah lattice ichneumon wasp of described pending Diapause inducement specifically can be ovum or 1 instar larvae of beet webworm Ah lattice ichneumon wasp, the time of induction described in step (1) can be 2 days.
In one embodiment of the invention, in step (2), described relative air humidity is 60%-70%.
In first two method provided by the present invention, within the time of storage described in step (2) can be 120 days, within being preferably 80 days.
In one embodiment of the invention, in step (3), the intensity of illumination in described condition is 4000 ~ 5000Lx; Relative air humidity in described condition is 70%-80%.
In one embodiment of the invention, the time of recovering described in step (3) to grow specifically can be 20 days.
The method of regulation and control beet webworm Ah lattice ichneumon wasp provided by the present invention diapause, Diapause inducement rate nearly 100%, the storage period after diapause can reach 120 days, within after Diapause termination 20 days, can obtain the one-tenth honeybee sprouted wings.The present invention not only contributes to the vast propagation promoting beet webworm Ah lattice ichneumon wasp in production practices, extends its product shelf phase and the prevention and control action time in field, also contributes to the development characteristics grasping natural enemy insect, improves control of insect efficiency.
Accompanying drawing explanation
Fig. 1 affects the diapause of beet webworm Ah lattice ichneumon wasp temperature and photoperiod.
Fig. 2 is the sensitive period analysis result that beet webworm Ah lattice ichneumon wasp experiences diapause signal.
Fig. 3 is the impact of different cold preservation time on beet webworm Ah lattice ichneumon wasp diapause cocoon.
Embodiment
Material used in following embodiment, reagent etc., if no special instructions, all can obtain from commercial channels.
For examination worm source:
Beet webworm: be recorded in " different beauveria bassianas to the toxicity test of beet webworm, Wu Jinhua, Liu Aiping etc. " Chinese plant protection guide " the 10th phase in 2011 " literary composition, the public can obtain from grassland research institute, China Agriculture academy of sciences.Beet webworm is as supplying examination host, climatic cabinate in laboratory (23 DEG C, L16:8D, light intensity 8800Lx) in use pea seedlings continuously rearing, the potted plant being developed to 5-6 sheet leaf is chosen when pea seedlings is used as host's food, clip cauline leaf raises beet webworm larva, every day feeds 1-2 time, in order to reduce the death of larva, when should reduce replacing blade as far as possible, the brush of larva is hindered or stirred, shake gently or concussion blade is adopted to make larva self falling on fresh blade, 1 age ~ 2 instar larvaes residing for ambient humidity remain on 70%-80%, residing for 3-5 instar larvae, environment remains on about 60%.
Beet webworm Ah lattice ichneumon wasp: be recorded in " beet webworm parasitic wasp and the control action to Host biotypes thereof, Tian Xiaoxia, Chinese Academy of Agricultural Sciences's Master's thesis, 2010 " literary composition, the public can obtain from grassland research institute, China Agriculture academy of sciences.Climatic cabinate (23 DEG C, RH60%, L16:8D, light intensity 8800Lx) interior with beet webworm 3-5 instar larvae even generation raising beet webworm Ah lattice ichneumon wasp, honeybee worm is than being 1:40, let alone abundant ovipositing hosts, observe day by day, after beet webworm larva buries and spins, every day sprays suitable quantity of water, keep the certain humidity of soil, treat that it is sprouted wings under being placed in old terms, the beet webworm Ah lattice ichneumon wasp of getting in emergence 24h waits to try.
Test apparatus and material
The temperature and light cycle tests all in climatic cabinate (SPX-300I-G type the impact of beet webworm Ah lattice ichneumon wasp Diapause inducement, Shanghai Medical Equipment Plant of Bo Xun Industrial Co., Ltd.) in carry out, climatic cabinate temperature step-up error is ± 1 DEG C, and relative moisture step-up error is ± 5%.The raising of beet webworm larva and beet webworm Ah lattice ichneumon wasp is all carried out in self-control dependent insect cage, connects and raises in large dependent insect cage (45cm × 45cm × 30cm) for population, and in test, little dependent insect cage specification used is that 30cm × 20cm × 20cm(is long × wide × high).Separately need flat based tubes (6cm × 1.5cm), mercurial thermometer, writing brush, absorbent cotton, elastic tape, pallet, culture dish, preservative film, vermiculite etc. are some.
The experimental technique used in following embodiment if no special instructions, is conventional method.
When following embodiment implementation data is analyzed, all utilize DPS to carry out variance analysis, significance test of difference adopts Duncans duncan's new multiple range method.
In following embodiment, photoperiod Lx:Dy represents light irradiation time x hour every day, dark duration y hour, x+y=24.
Following embodiment all adopts international parasitic wasp diapause criterion, and general principle is based on the rule of effective temperature summation, when certain worm state of certain parasitic wasp remains unfulfilled growth within the twice time that should complete development duration, namely assert it is enter diapause status.More concrete, judge in the present invention whether beet webworm Ah lattice ichneumon wasp enters diapause status and determine as follows: if this worm state of beet webworm Ah lattice ichneumon wasp " pupa " remains unfulfilled growth within the twice time (namely 30 days) that should complete development duration, namely from larva buries and spins, in 30 days, beet webworm Ah lattice ichneumon wasp fails to sprout wings, then assert that beet webworm Ah lattice ichneumon wasp enters diapause status.
The Diapause inducement of embodiment 1, beet webworm Ah lattice ichneumon wasp
One, the determination in beet webworm Ah lattice ichneumon wasp diapause temperature and light cycle
Adopt two-factor orthogonal experiment, 5 different photoperiod (L8:D16 are set, L10:D14, L12:D12, L14:D10, L16:D8) and 4 different temperatures (17 DEG C, 20 DEG C, 23 DEG C, 26 DEG C), totally 20 process, each process 30 beet webworm larvas (host), repeat 3 times, beet webworm Ah lattice ichneumon wasp queen bee after mating 1d is parasitized beet webworm larva, then proceed to and be equipped with in the insect box of sandy soil, put into the climatic cabinate of different photoperiod and Temperature Treatment (wherein, intensity of illumination during illumination is 4000 ~ 5000LX, relative air humidity is 70%-80%), Diapause inducement (Diapause inducement is considered as from " ovum " this worm state) is carried out to beet webworm Ah lattice ichneumon wasp, induction time is 2 days, the normal rearing conditions of beet webworm Ah lattice ichneumon wasp is transferred to after induction, i.e. climatic cabinate (23 DEG C, RH60%, L16:8D, light intensity 8800Lx).In experiment, every day provides fresh pea seedlings for beet webworm larva.The growth of observed and recorded beet webworm larva (host) and beet webworm Ah lattice ichneumon wasp queen bee (parasitic wasp) developmental state, the formation of statistics parasitic wasp Diapause and temperature and the relation between the photoperiod.
Result is as shown in table 1 and Fig. 1, and under temperature is 17 ~ 26 DEG C of conditions, within the scope of light application time 8 ~ 16h, along with the reduction of temperature and the shortening of light application time, Diapause rate obviously increases.Temperature or photoperiod all have significant impact to beet webworm Ah lattice ichneumon wasp Diapause rate.As can be seen from Table 1, when temperature is 26 DEG C, light application time 8 ~ 16h, does not have diapause phenomenon to occur; Temperature is 23 DEG C, light application time 12 ~ 8h, the individual diapause (0.7% ~ 13.6%) of minority; When temperature is 20 DEG C, Diapause rate significantly improves, and the Diapause rate of light application time 16 ~ 8h is respectively 2.8%, 8.6%, 61.6%, 70.3% and 90.5%; When mean temperature is 17 DEG C, light application time is lower than 12h, then the individuality of 100% enters diapause.As can be seen from Figure 1, low temperature, short photoperiod are the principal elements of induction beet webworm Ah lattice ichneumon wasp diapause, and temperature plays a leading role, and the photoperiod only works in certain temperature range; 26 DEG C time, short photoperiod can not induce diapause.
Under table 1 indoor conditions, the temperature and light cycle is on the impact of beet webworm Ah lattice ichneumon wasp diapause
Note: in table, data are mean value ± standard errors, after data, different lowercase alphabet shows significant difference (p<0.05), and different capitalization represents difference extremely significantly (p<0.01) (Duncan ' s duncan's new multiple range method).
Two, the determination of beet webworm Ah lattice ichneumon wasp diapause regulation and control life stages sensitive
According to the light temperature Diapause inducement result in step one, at temperature 17 DEG C, photoperiod L8: D16(short photoperiod) diapause environment, and temperature 23 DEG C, photoperiod L16: D8(long illumination) non-diapause environment, intensity of illumination is 4000 ~ 5000LX, relative air humidity is tested under being the condition of 70%-80% as follows:
A. will be put into above-mentioned non-diapause environment by the beet webworm larva (host) of beet webworm Ah lattice ichneumon wasp parasitism on the same day, process 0,2,4,6,9, after 13d, to transfer in above-mentioned diapause environment 2 days.40 host larvas are established in each process, repeat 3 times, record the quantity of non-diapause cocoon and diapause cocoon.
B. by the same day by the respectively process of the beet webworm larva (host) of beet webworm Ah lattice ichneumon wasp parasitism in above-mentioned diapause environment 3,6,9,12,16, after 18d, transfer in above-mentioned non-diapause environment.Each process 40 host larvas, repeat 3 times, record the quantity of non-diapause cocoon and diapause cocoon.
Result is as shown in table 2 and Fig. 2, if first grown 2 days in non-diapause environment by the host larva of parasitism, then transfer in diapause environment, the Diapause rate of cocoon is 100%, and now beet webworm Ah lattice ichneumon wasp is 1 instar larvae; Grow and within 6 days, transfer in diapause environment, the Diapause rate of cocoon drops to 4.5%, grows and moves on in diapause environment for more than 9 days again, do not have diapause to occur.If first grown by the host larva of parasitism 6 days in diapause environment, transfer in non-diapause environment, the Diapause rate of cocoon is 0; Grow under within 9 days, transferring to non-diapause condition again, Diapause rate is 5.9%, and now beet webworm is 3 instar larvaes; Grow under within more than 12 days, transferring to non-diapause condition again, Diapause rate 100%.Thus, the life stages sensitive that beet webworm Ah lattice ichneumon wasp experiences diapause signal is ovum and 1 instar larvae, after ovum and 1 instar larvae experience diapause signal, needs to grow under diapause condition all to enter diapause to mature larva.
Table 2 beet webworm Ah lattice ichneumon wasp experiences the sensitive period of diapause signal
Note: in table, data are mean value ± standard errors, after data, different lowercase alphabet shows significant difference (p<0.05), and different capitalization represents difference extremely significantly (p<0.01) (Duncan ' s duncan's new multiple range method).Wherein, " developmental stage " is by dissecting rear discovery to polypide: dissect from host 3 instar larvae, 5 larvas are dissected every 2d, treat that larva buries to continue to dissect, observe the worm state of pin main body internal excited state, under stereomicroscope, observe parasitoid larva developmental condition in each in length of time and determine development time, result is as follows: in 23 DEG C of non-diapause situations, Ah lattice ichneumon wasp experiences about 13 days by egg development to prepupa, and the development time of 1 age, 2 ages, 3 ages, 4 ages, 5 instar larvaes is respectively 2d, 2d, 3d, 2d, 2d; In 17 DEG C of diapause situations, experience about 18 days by egg development to prepupa, the development time of 1 age, 2 ages, 3 ages, 4 ages, 5 instar larvaes is respectively 3d, 3d, 3d, 4d, 2d.
The result of integrated embodiment 1, as seen for beet webworm Ah lattice ichneumon wasp ovum or 1 instar larvae, imposes temperature 17 DEG C, the envirment factor of photoperiod L8:D16 to L12:D12 stimulates 2 days, 100% individuality can be induced to enter diapause state.
The diapause maintenance of embodiment 2, beet webworm Ah lattice ichneumon wasp
The present embodiment is by measuring the eclosion rate of beet webworm Ah lattice ichneumon wasp diapause cocoon, and the life-span of one-tenth honeybee after sprouting wings, egg-laying period and parasitic rate, determines the time of low-temperature storage beet webworm Ah lattice ichneumon wasp diapause cocoon.Specific as follows:
1, the eclosion rate of beet webworm Ah lattice ichneumon wasp diapause cocoon
The beet webworm Ah lattice ichneumon wasp (now outward appearance is worm cocoon) of the diapause state adopting the method for embodiment 1 to obtain is collected in moistening soft soil, be dispensed into (diameter 10cm in plastic bowl, high 8cm), the ventilative gauze of plastic bowl covers, be stored in full dark, refrigerating box at 4 DEG C, spray in an every 3 days water, maintains relative air humidity 60%-70%.Regularly diapause cocoon is taken out from refrigerator and put into temperature 23 DEG C, photoperiod L14: D10 incubator carries out catalysis, observe and add up the eclosion rate refrigerating parasitic wasp diapause cocoon after different time.Experiment establishes 8 process (8 cold preservation times, in table 3), each process 40 ~ 60 diapause cocoons altogether, repeats 3 times.
2, the beet webworm Ah lattice ichneumon wasp after sprouting wings becomes life-span of honeybee, egg-laying period and parasitic rate
One-tenth honeybee after diapause cocoon emergence step 1 obtained puts into insect box, a pair beet webworm Ah lattice ichneumon wasp (1 queen bee+1 drone) introduced by every box, with the nutrition as a supplement of the hydromel of mass fraction 10%, mating 24h under the condition of temperature 23 DEG C, photoperiod L14: D10, then inoculate, parasitize beet webworm larva by the beet webworm Ah lattice ichneumon wasp queen bee after mating 1d.Each process inoculation 4 ~ 5 pairs of parasitic wasps, repeat 3 times.Often pair of honeybee inoculates 50 beet webworm larva confession in 4 ages queen bees parasitisms, takes out female drone and continue inoculation, until female drone death after 24h.With the non-diapause cocoon of non-chilling treatment for contrast (CK).Add up the life-span of the one-tenth honeybee after sprouting wings, egg-laying period and parasitic rate.Wherein, the egg-laying period observes the time interval between laying eggs for the first time and laying eggs for the last time, usually daily calculates in practical operation.Parasitic rate is divided by total beet webworm number by the beet webworm number of parasitism.
3, result
Result is as shown in table 3 and Fig. 3, the beet webworm Ah lattice ichneumon wasp diapause cocoon adopting the method for embodiment 1 to obtain is transferred to 23 DEG C of catalysis about 4 DEG C refrigerations after 80 days, its eclosion rate is 80.5%, male about 13 days peak life-spans, about 14 days queen bee life-spans, lay eggs 4.4 days duration, parasitic rate reaches 30.8%, not remarkable with control group difference.Diapause cocoon refrigerates 120 days, although the indexs such as female drone life-span, egg-laying period are starkly lower than control group, but still have the diapause cocoon of 71.7% normally to sprout wings, in about 3 days egg-laying periods, parasitic rate is 20.4%.
Table 3 refrigerates the impact on diapause cocoon
Note: in table, data are mean value ± standard errors, after data, different lowercase alphabet shows significant difference (p<0.05), and different capitalization represents difference extremely significantly (p<0.01) (Duncan ' s duncan's new multiple range method).
The result of cumulated volume embodiment, as seen for the beet webworm Ah lattice ichneumon wasp (diapause cocoon) entering diapause state, under being stored in full dark, 4 DEG C of conditions, storage period can reach 120 days.
The Diapause termination of embodiment 3, beet webworm Ah lattice ichneumon wasp
For diapause state beet webworm Ah lattice ichneumon wasp (diapause cocoon) adopting the method for embodiment 1 to obtain, by thermal stimulus, termination of diapause state, enters development process, and concrete measure is as follows:
Beet webworm Ah lattice ichneumon wasp diapause cocoon is placed in the environment of temperature 23 DEG C, photoperiod L14:D10 to L16:D8, luminous intensity 4000-5000Lx, spray in an every 3 days water, keep certain humidity (RH is 70%-80%), get final product termination of diapause state after 20 days, diapause cocoon all turns into into honeybee very soon.
Wherein, induction time is determined for 20 days as follows:
Diapause termination is carried out by adopting the method for embodiment 2 storage beet webworm Ah lattice ichneumon wasp diapause cocoon after 120 days under full dark, 4 DEG C of conditions to proceed in temperature 23 DEG C, the incubator of photoperiod L14: D10 (RH is 70%-80%), the emergence time of statistics parasitic wasp diapause cocoon, adopt 60 diapause cocoons to test, establish altogether and repeat experiment for 3 times.Result shows, and from starting to carry out Diapause termination under diapause cocoon being proceeded to 23 DEG C of conditions, after about 20 days, diapause cocoon all turns into into honeybee very soon, termination of diapause state.Repeat experimental result indifference three times.
Claims (2)
1. regulate and control a method for beet webworm Ah lattice ichneumon wasp diapause, comprise the steps:
(1) induce under the beet webworm Ah lattice ichneumon wasp of pending Diapause inducement being placed in the condition of temperature 17 ± 1 DEG C, photoperiod L8:D16 to L12:D12, make described beet webworm Ah lattice ichneumon wasp enter diapause state;
(2) the described beet webworm Ah lattice ichneumon wasp entering diapause state step (1) obtained stores under proceeding to temperature 4 DEG C, dark condition;
(3), after step (2) storage terminates, under described beet webworm Ah lattice ichneumon wasp is proceeded to the condition of temperature 23 DEG C, photoperiod L14:D10 to L16:D8, recover to grow;
The beet webworm Ah lattice ichneumon wasp of described pending Diapause inducement is ovum or 1 instar larvae of beet webworm Ah lattice ichneumon wasp;
Described in step (1), the time of induction is 2 days;
Described in step (2), the time of storage is within 120 days;
The time of recovering described in step (3) to grow is 20 days.
2. method according to claim 1, is characterized in that: the time of described storage is within 80 days.
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| CN1273027A (en) * | 2000-06-15 | 2000-11-15 | 河北省农林科学院植物保护研究所 | Artificial reproduction method for Microplitis mediator (Haliday) |
| CN100450351C (en) * | 2006-03-28 | 2009-01-14 | 衡水田益生防有限责任公司 | Standard artificial breeding technology for short tongue bumblebee |
| CN101326902B (en) * | 2008-07-04 | 2011-05-04 | 衡水田益生防有限责任公司 | Method for inducing diapause of Trichogramma ostriniae by constant temperature and storage method |
| CN101720724B (en) * | 2009-12-04 | 2012-02-08 | 沈阳农业大学 | Long-term storage method of bee species of artificially diapaused and induced Chouioia cunea |
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| 生物因子对寄生蜂滞育的影响;李玉艳 等;《昆虫知识》;20100715;第47卷(第4期);639-643 * |
| 草地螟的发生规律及综合防治;郭成 等;《昭乌达蒙族师专学报》;20030430;第24卷(第2期);26,31 * |
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