CN105432568A - Method for artificially feeding drosophila suzukii matsumura - Google Patents
Method for artificially feeding drosophila suzukii matsumura Download PDFInfo
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- CN105432568A CN105432568A CN201510777641.2A CN201510777641A CN105432568A CN 105432568 A CN105432568 A CN 105432568A CN 201510777641 A CN201510777641 A CN 201510777641A CN 105432568 A CN105432568 A CN 105432568A
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- 238000000034 method Methods 0.000 title claims abstract description 22
- 241001136566 Drosophila suzukii Species 0.000 title abstract 3
- 239000011521 glass Substances 0.000 claims abstract description 20
- 230000003203 everyday effect Effects 0.000 claims abstract description 18
- 230000001954 sterilising effect Effects 0.000 claims abstract description 13
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 9
- 239000008223 sterile water Substances 0.000 claims abstract description 7
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- 238000009395 breeding Methods 0.000 claims description 20
- 230000001488 breeding effect Effects 0.000 claims description 20
- 102000002322 Egg Proteins Human genes 0.000 claims description 17
- 108010000912 Egg Proteins Proteins 0.000 claims description 17
- 235000013601 eggs Nutrition 0.000 claims description 17
- 210000004681 ovum Anatomy 0.000 claims description 17
- 230000000384 rearing effect Effects 0.000 claims description 11
- 241000382353 Pupa Species 0.000 claims description 9
- 235000005911 diet Nutrition 0.000 claims description 9
- 230000037213 diet Effects 0.000 claims description 9
- 230000001418 larval effect Effects 0.000 claims description 9
- 230000017448 oviposition Effects 0.000 claims description 9
- 239000012528 membrane Substances 0.000 claims description 5
- 230000019617 pupation Effects 0.000 claims description 5
- 238000007789 sealing Methods 0.000 claims description 5
- 230000032683 aging Effects 0.000 claims description 4
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- 238000005286 illumination Methods 0.000 claims description 4
- 244000157072 Hylocereus undatus Species 0.000 claims description 3
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- 230000001228 trophic effect Effects 0.000 claims description 3
- 241000238631 Hexapoda Species 0.000 abstract description 12
- 238000011161 development Methods 0.000 abstract description 3
- 238000004659 sterilization and disinfection Methods 0.000 abstract 2
- 230000004634 feeding behavior Effects 0.000 abstract 1
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- 238000007920 subcutaneous administration Methods 0.000 description 3
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- 239000008272 agar Substances 0.000 description 2
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- 230000018109 developmental process Effects 0.000 description 2
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- 208000003322 Coinfection Diseases 0.000 description 1
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Feeding And Watering For Cattle Raising And Animal Husbandry (AREA)
- Catching Or Destruction (AREA)
Abstract
The invention discloses a method for artificially feeding drosophila suzukii matsumura, wherein adults which are feathered in the same day are selected to put into a self-made adult feeding cage, 5.5g artificial feed is stored in a culture dish A, then the culture dish A is arranged in a culture dish B which is paved with a sterilization filter paper, sterile water is injected on the sterilization filter paper which is arranged in the culture dish B, the culture dish B which is matched with the culture dish A is put into the self-made adult feeding cage, and is put into a phytotron to observe and count number of egg grains, the culture dish A is put into a glass beaker, and is sealed through a Para film and then is put into the phytotron to feed, the growth, the development and the feeding behavior of feed insects are observed in a stereomicroscope, are observed until larva are aged in fixed time every day, the adults which are feathered in the same day are conjugated according to the female-male ratio of 1:1, and the conjugated adults are respectively put into the adult feeding cage to feed. The method for artificially feeding the drosophila suzukii matsumura is simple, convenient in operation and easy to feed targeted insects.
Description
Technical field
The invention belongs to technical field of insect feeding, relate to the rearing method of a kind of Ling Mushi fruit bat.
Background technology
Ling Mushi fruit bat (DrosophilasuzukiiMatsumura), also known as cherry fruit bat, spot samara fly, be subordinate to diptera (Diptera) circular row suborder (Cylorrhapha) Drosophilidae (Drsophilidae) Drosophila (Drosophila) fruit fruit bat subgenus (SubgenusSophophora).
Ling Mushi fruit bat is holometabola insect, and all one's life comprises ovum, larva, pupa and four periods of adult.Because this worm individuality is less, and the life area in all one's life is relatively hidden, at initial stage that this worm is caused harm and being very not easily found in early days.Adult small (long about the 2-3mm of body), health is soft, and adult trends towards selecting the hidden gap of fruit and wound to carry out taking food, laying eggs, or female adult pest is selected, and ripened fruit is directly subcutaneous to lay eggs; Newly hatched larvae is damage in fruit directly, and the whole larval phase of this worm all bore in fruit and eat into, take food, grow, grow, until larva is aging, part mature larva departs from fruit pupates, another part mature larva is pupated on fruit top layer, pupates in also dry under the fruit peel place had.A large amount of larva and larval feeding tunnel and metabolism ight soil is covered with in the fruit that this worm was caused harm, therefore, the edibility of serious loss fruit itself and economic worth.
It is reported, nineteen thirty because Ling Mushi fruit bat is caused harm, causes the wine-growing damage of local all cherries and 80% in Japan.Cut-off nineteen ninety-five, the cherry plantation of Japanese some areas 26%-100% is subject to causing harm of this worm.This worm is classified as one of blueberry of causing harm insect the most serious by Japan.This worm has caused the cherry of 80% and the blueberry underproduction of 40% when the U.S. is the most serious.Hauser and Damus research finds that this female adult pest mainly selects ripened fruit to carry out subcutaneous laying eggs, and the part of laying eggs easily addles, and causes harm create prerequisite for pathogen, secondary pathogen and other insect carry out Secondary Infection to fruit.
Lay eggs in the hidden gap of fruit due to this worm and directly lay eggs under fruit peel, its larva moves in fruit pulp, gives the biological property such as growth, growth of directly this worm of observation, and prevents and treats this insect and bring larger difficulty.Therefore, study the biological property of this worm, the pheromones that research and development are effectively practical, find the good natural enemy insect of control effects imperative.In recent years, Ling Mushi fruit bat along with international, domestic trade contact is frequent, worldwide, except the natural diffuseness in suitable nature or management environment, have and propagate with Commodity Transportation and carrying of the vehicles, also have and carry out collaborative diffusion with the pest of potential risk for carrier, also have and extensively to be spread by border belt trading commodity, be more carried on agricultural byproducts and discarded object etc. and distribute rapidly diffusion.
Therefore, study the biological property of this worm, pheromones, imago attractant that research and development are effectively practical, find the good natural enemy insect of control effects extremely urgent especially.
People's publicities such as in July, 2014 " Plant Protection Institute, Shandong Academy of Agricultural Sciences " Zhai Yifan " a kind of Ling Mushi fruit bat Prof. Du Yucang feed ".On the basis coordinating artificial feed, Shandong academy of agricultural sciences also creates the matching method " a kind of rearing method for examination Ling Mushi fruit bat " having ground a set of indoor feeding Ling Mushi fruit bat, application number: 201410163572.1, on 04 22nd, 2014 applying date.
But herein inventor at laboratory internal reference " the feed patent " and " process patent " of Shandong academy of agricultural sciences publicity, try worm a large amount of each length of time that could not obtain preferably required for experiment.Through analysis and summary, following problem may be there is:
1. may because the difference of region, the kind of the material of thus testing (apple and banana etc.) is variant, causes feeding effect not good.
2. in the production process of feed, may also there are some differences, also cause feeding effect undesirable (do not have the program of feed making in the material of publicity, also do not have the moisture parameter of adding in feed).
3., because region is different and the difference of test period, this paper inventor goes through test of many times in laboratory, and result shows that the method is not ideal enough to feed surface moistening effect, causes the easy air-dry dehydration of feed surface, is unfavorable for taking food and laying eggs of fruit bat.In addition, with the addition of agar in feed, shaping feed is solid block, and feed surface is easy to air-dryly affect taking food and laying eggs of fruit bat, even if at the filter paper of feed lower berth moisture releasing profit, to the time of feed surface moisturizing and the effect of moisturizing also not ideal enough.
4. due to the particularity (lay eggs under fruit peel, lay eggs in fruit gap, fruit wound is laid eggs) of Ling Mushi fruit bat spawning habit, feed in solid fraction is because there is certain hardness, test shows that its ovum grain fecund is in the surface of feed, thus is unfavorable for the hatching of ovum grain.
5. Ling Mushi fruit bat whole larval phase, all lives in the higher fruit of water content (berry) pulp, and therefore larva requires that living environment is easy to its moth food, activity.The agar feed of solid fraction limits moth food, the activity of larva, is also unfavorable for the biological property observing larval phase at any time.Need if any observation, solid fraction feed must be cut open, both destroyed the living environment of this worm, be very easy to again damage target polypide, affect growing of this worm, thus affect test effect.
Summary of the invention
The object of this invention is to provide the rearing method of a kind of Ling Mushi fruit bat, solve problems of the prior art, the method is simple, easy to operate, targeted insect easily raises.
The technical solution adopted in the present invention is, the rearing method of a kind of Ling Mushi fruit bat, specifically carries out according to following steps:
One, the adult stage
Step 1, chooses the 5 pairs of adults sprouted wings the healthy same day and puts into homemade imago breeding cage;
Step 2 is that culture dish A contains 5 grams of meridic diets with the diameter 3cm of sterilizing, then is placed in by culture dish A and is lined with the culture dish B that diameter is the sterilizing filter paper of 11cm, culture dish B diameter 12cm;
Step 3, the filter paper in culture dish B injects 5ml sterile water; Every 3-6h injects 5ml sterile water in culture dish B later;
Step 4, then the culture dish B being furnished with culture dish A is put into imago breeding cage, for imago feeding, lay eggs;
Step 5, imago breeding cage is put into phytotron, and every day, timing to swap out little culture dish A with the little culture dish of the diameter 3cm that new feedstuff is housed, under little culture dish A is put into stereomicroscope, observe statistics ovum grain number, for the day egg laying amount and single female egg laying amount calculating Ling Mushi fruit bat;
Two, raise in larval phase, pupa time
Step 6, the culture dish A adding up ovum grain number will be observed under stereomicroscope, put into the 50-100 milliliter glass beaker of sterilizing, with Parafilm ParafilmTM glass beaker mouth, and air-vent is pricked on sealed membrane, and on glass beaker numbering mark respectively, then put into phytotron and raise;
Step 7, timing every day takes out culture dish A according to numbering mark, observe under stereomicroscope raise worm growth, growth and trophic behaviour;
Step 8, correspond to the playback of numbering mark, sealing, then is put into phytotron by the culture dish A examined under a microscope;
Step 9, as the tracing study of needs single head is raised, only leaves healthy one by the ovum grain in little culture dish A;
Step 10, every day, timing was observed until larva is aging, climbed out of in the ware wall or glass beaker that feed is bonded at culture dish A, just can directly raise the pupating of worm through glass beaker observation, sprout wings;
Raise after 4-5 days, observed and recorded larvae pupation rate, within 8-10 days, observed and recorded raises eclosion rate corresponding to worm, male worm number, female borer population;
Step 11, the adult sprouted wings the same day is 1:1 pairing according to sex ration, is rendered to respectively in imago breeding cage by the adult matched and raises;
Step 12, if any single needs to the raising of adult, by single for the adult of pairing to rendering to an imago breeding cage, to carry out observed and recorded.
Further, described imago breeding cage specification: 40 cm x 40 cm x 40 centimetres.
Further, the environmental condition of phytotron in described step 5, step 6: temperature 25 DEG C, relative moisture 75%, illumination every day 12 hours, dark 12 hours.
Further, described step 2 meridic diet be dragon fruit obtained partly freeze shape meridic diet.
Beneficial effect of the present invention:
1. adopt this research, can at any time, direct, easy, the growth and development state of following the tracks of ground observation ovum, larva, pupa and adult, for the indoor life table making this worm provides fairly simple, convenient, reliable method.
2. this research can improve the accuracy rate of the biology index of correlation data statistics such as examination worm day egg laying amount, single female egg laying amount, incubation rate, pupation rate, survival rate effectively.
3. this research can provide abundant living feed for the research of the natural enemy insect of Ling Mushi fruit bat, exploitation, raising, breeding.For the researchs such as Ling Mushi fruit bat polypide pheromones extraction are laid solid, basic reliably.
4. the method for this research can promote the use of fruit moth feeding habits is the research of insect.As: fruit bat class, trypetid class, heart-eating worm class (peach fruit borer etc.).
Embodiment
A rearing method for Ling Mushi fruit bat, specifically carries out according to following steps:
One, the adult stage
Step 1, chooses the 5 pairs of adults sprouted wings the healthy same day and puts into homemade imago breeding cage, imago breeding cage specification: 40 cm x 40 cm x 40 centimetres;
Step 2 is that little culture dish A contains 5 grams of meridic diets with the diameter 3cm of sterilizing, then is placed in by culture dish A and is lined with the culture dish B that diameter is the sterilizing filter paper of 11cm, culture dish B diameter 12cm;
Step 3, the filter paper in culture dish B injects 5ml sterile water, has both ensured that filter paper is fully wetting, guarantees again to have table water in culture dish B, guarantees raised adult drinking-water and feed moisturizing, also meets fruit bat simultaneously and require higher air humidity; Every 3-6h injects 5ml sterile water in culture dish B later;
Step 4, then the culture dish B being furnished with little culture dish A is put into imago breeding cage, for imago feeding, lay eggs;
Step 5, puts into phytotron by imago breeding cage, environmental condition: temperature 25 DEG C, relative moisture 75%, illumination every day 12 hours, and dark is raised for 12 hours; Every day, timing to swap out little culture dish A with the little culture dish of the diameter 3cm that new feedstuff is housed, and under little culture dish A is put into stereomicroscope, observed statistics ovum grain number, for the day egg laying amount and single female egg laying amount calculating Ling Mushi fruit bat;
Two, raise in larval phase, pupa time
Step 6, the little culture dish A adding up ovum grain number will be observed under stereomicroscope, put into the 50-100 milliliter glass beaker of sterilizing, glass beaker mouth is sealed with Parafilm sealed membrane (brand: U.S. Parafilm), and small air-vent on pricking on sealed membrane, and on beaker numbering mark respectively, then put into phytotron and raise; Environmental condition: temperature 25 DEG C, relative moisture 75%, illumination every day 12 hours, dark 12 hours;
Every two hours observed and recorded once, the larvae hatch of about about 90%;
Step 7, timing every day takes out little culture dish A according to numbering mark, observes and raise the growth of worm, growth and trophic behaviour under stereomicroscope;
The little culture dish A in glass beaker can be taken out if desired, be put into observe under stereomicroscope cast off a skin, oral hook dropping situations;
Step 8, correspond to the playback of numbering mark, sealing, then is put into climatic cabinate by the little culture dish A examined under a microscope;
Step 9, as the tracing study of needs single head is raised, only leaves healthy one by the ovum grain in little culture dish A;
Step 10, every day, timing was observed until larva is aging, climbed out of in the ware wall or glass beaker that feed is bonded at little culture dish A, just can directly raise the pupating of worm through glass beaker observation, sprout wings;
Raise after 4-5 days, observed and recorded larvae pupation rate, within 8-10 days, observed and recorded raises eclosion rate corresponding to worm, male worm number, female borer population;
Step 11, the adult sprouted wings the same day is 1:1 pairing according to sex ration, is rendered to respectively in imago breeding cage by the adult matched and raises;
Step 12, if any single needs to the raising of adult, by single for the adult of pairing to rendering to an imago breeding case, to carry out observed and recorded.
The present invention utilizes: 1. adult has obvious positive taxis to red (cerise and peony); 2. adult trends towards the habit that the hidden gap of fruit and wound carry out taking food, laying eggs; 3. female adult pest is also chosen to ripe fruit and directly carries out subcutaneous habit of laying eggs; To have made with " red heart dragon fruit " be main raw material(s) partly freezes shape meridic diet in screening.
Provide the character of feed of single head raising Ling Mushi fruit bat, complete container and sealing tech first.The proterties of feed can be guaranteed that safety is observed at any time and raise worm, does not impact raising worm and damages; Complete container and sealing tech can guarantee feed desiccation, and in good isolate forster environment, pathogen (bacterium and fungal spore) grows the predation etc. with natural enemy (predation spider etc.).This invention guarantee researcher directly observe and grasp raise worm whole ovum phase, larval phase, the taking food of pupa time and adult stage, grow, biology and the behaviouristics characteristic such as growth, also be other boring pest of research, and the research of its parasitism and predator provide reliable solid foundation; This invention efficiently solves: the life of Ling Mushi fruit bat is more hidden, its biological property of observational study and the difficult problem such as behaviouristics characteristic is more difficult.And lay solid, basis reliably for the research such as Ling Mushi fruit bat polypide pheromones extraction.
Embodiment 1
In September, 2014, take 5 grams of bases and partly freeze shape self-control feed in the sterilizing culture dish A of diameter 3 centimetres, by the culture dish A of band feed, every day, 16:00-22:00 put into imago breeding cage, lay eggs for imago feeding, every day belt replacement feed culture dish, collect the data that record egg laying amount is relevant.The culture dish A of band ovum grain is put into sterilized clear glass beaker (beaker 50-100ml), with Parafilm ParafilmTM beaker mouth, and breathable microporous on pricking on film.Again the glass beaker be equipped with is put into climatic cabinate and raise 24 hours (rearing conditions: 25 DEG C, relative moisture is 75%, within one day 24 hours, to turn on light light application time: interlunation of turning off the light is 12:12), every two hours observed and recorded once, the larvae hatch of about about 90%.If any single head raising need, newly hatched larvae access is filled finger-type glass tube (often pipe 1 larva of 0.2 gram of feed by the refined toothpick of available sterilizing, totally 50 pipes), seal with Parafilm sealed membrane, continue to put into climatic cabinate to raise, timing every day observed and recorded larval behavior, g and D; Or a healthy newly hatched larvae is only stayed in the culture dish A containing feed, culture dish A is put into sterilized clear glass beaker (beaker 50-100ml), with Parafilm ParafilmTM beaker mouth, and breathable microporous on pricking on film, timing every day observed and recorded larval behavior, g and D.About the 8th day larvae pupation, about the 12nd day, pupa turned into adult.
Embodiment 2:
In October, 2014 repeats the content of embodiment 1.
Embodiment 3:
In November, 2014 repeats the content of embodiment 1 again.
Embodiment 4:
In December, 2014 repeats the content of embodiment 1 again.
The development duration of table 1 diet Ling Mushi of the present invention fruit bat
As can be seen from Table 1:
(1) Ling Mushi fruit bat is raised with raising method of the present invention, can the biology in ovum phase of this fruit bat of tracing study, larval phase, pupa time and adult stage and behaviouristics characteristic at any time;
(2) test data shows: this raising method effect is better, and the survival rate of ovum, larva, pupa is also more satisfactory.Obviously be better than the effect of the conventional raising method with fruit;
(3) scope of application of the present invention: the fruit bat class of fruit of causing harm, trypetid class and heart-eating worm class insect.
Claims (4)
1. a rearing method for Ling Mushi fruit bat, is characterized in that, specifically carries out according to following steps:
One, the adult stage
Step 1, chooses the 5 pairs of adults sprouted wings the healthy same day and puts into homemade imago breeding cage;
Step 2 is that culture dish A contains 5 grams of meridic diets with the diameter 3cm of sterilizing, then is placed in by culture dish A and is lined with the culture dish B that diameter is the sterilizing filter paper of 11cm, culture dish B diameter 12cm;
Step 3, the filter paper in culture dish B injects 5ml sterile water; Every 3-6h injects 5ml sterile water in culture dish B later;
Step 4, then the culture dish B being furnished with culture dish A is put into imago breeding cage, for imago feeding, lay eggs;
Step 5, imago breeding cage is put into phytotron, and every day, timing to swap out little culture dish A with the little culture dish of the diameter 3cm that new feedstuff is housed, under little culture dish A is put into stereomicroscope, observe statistics ovum grain number, for the day egg laying amount and single female egg laying amount calculating Ling Mushi fruit bat;
Two, raise in larval phase, pupa time
Step 6, the culture dish A adding up ovum grain number will be observed under stereomicroscope, put into the 50-100 milliliter glass beaker of sterilizing, with Parafilm ParafilmTM glass beaker mouth, and air-vent is pricked on sealed membrane, and on glass beaker numbering mark respectively, then put into phytotron and raise;
Step 7, timing every day takes out culture dish A according to numbering mark, observe under stereomicroscope raise worm growth, growth and trophic behaviour;
Step 8, correspond to the playback of numbering mark, sealing, then is put into phytotron by the culture dish A examined under a microscope;
Step 9, as the tracing study of needs single head is raised, only leaves healthy one by the ovum grain in little culture dish A;
Step 10, every day, timing was observed until larva is aging, climbed out of in the ware wall or glass beaker that feed is bonded at culture dish A, just can directly raise the pupating of worm through glass beaker observation, sprout wings;
Raise after 4-5 days, observed and recorded larvae pupation rate, within 8-10 days, observed and recorded raises eclosion rate corresponding to worm, male worm number, female borer population;
Step 11, the adult sprouted wings the same day is 1:1 pairing according to sex ration, is rendered to respectively in imago breeding cage by the adult matched and raises;
Step 12, if any single needs to the raising of adult, by single for the adult of pairing to rendering to an imago breeding cage, to carry out observed and recorded.
2. the rearing method of a kind of Ling Mushi fruit bat according to claim 1, is characterized in that, described imago breeding cage specification: 40 cm x 40 cm x 40 centimetres.
3. the rearing method of a kind of Ling Mushi fruit bat according to claim 1, is characterized in that, the environmental condition of phytotron in described step 5, step 6: temperature 25 DEG C, relative moisture 75%, illumination every day 12 hours, dark 12 hours.
4. the rearing method of a kind of Ling Mushi fruit bat according to claim 1, is characterized in that, described step 2 meridic diet be dragon fruit obtained partly freeze shape meridic diet.
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| CN201510777641.2A CN105432568B (en) | 2015-11-13 | 2015-11-13 | A kind of rearing method of Ling Mushi drosophila |
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| CN201510777641.2A CN105432568B (en) | 2015-11-13 | 2015-11-13 | A kind of rearing method of Ling Mushi drosophila |
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| CN105432568B CN105432568B (en) | 2019-05-21 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105766812A (en) * | 2016-04-07 | 2016-07-20 | 云南省农业科学院农业环境资源研究所 | Method for breeding Drosophila suzukii Matsumura in captivity |
| CN108575920A (en) * | 2018-07-05 | 2018-09-28 | 中国农业科学院植物保护研究所 | A kind of breeding apparatus and method for breeding of phthorimaea operculella |
| CN112772573A (en) * | 2021-01-27 | 2021-05-11 | 广西壮族自治区农业科学院植物保护研究所 | Indoor artificial breeding method for acrossodes amabilis |
Citations (2)
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| CN103918887A (en) * | 2014-04-22 | 2014-07-16 | 山东省农业科学院植物保护研究所 | Artificial feed for suzukii fruit bat fly |
| CN103931565A (en) * | 2014-04-22 | 2014-07-23 | 山东省农业科学院植物保护研究所 | Artificial breeding method of Suzuki fruit flies for testing |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103918887A (en) * | 2014-04-22 | 2014-07-16 | 山东省农业科学院植物保护研究所 | Artificial feed for suzukii fruit bat fly |
| CN103931565A (en) * | 2014-04-22 | 2014-07-23 | 山东省农业科学院植物保护研究所 | Artificial breeding method of Suzuki fruit flies for testing |
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| 林清彩等: "铃木氏果蝇研究进展", 《江西农业学报》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105766812A (en) * | 2016-04-07 | 2016-07-20 | 云南省农业科学院农业环境资源研究所 | Method for breeding Drosophila suzukii Matsumura in captivity |
| CN108575920A (en) * | 2018-07-05 | 2018-09-28 | 中国农业科学院植物保护研究所 | A kind of breeding apparatus and method for breeding of phthorimaea operculella |
| CN112772573A (en) * | 2021-01-27 | 2021-05-11 | 广西壮族自治区农业科学院植物保护研究所 | Indoor artificial breeding method for acrossodes amabilis |
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