CN104054634B - A kind of artificial propagation techniques of Semen Caesalpiniae - Google Patents
A kind of artificial propagation techniques of Semen Caesalpiniae Download PDFInfo
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- CN104054634B CN104054634B CN201310290303.7A CN201310290303A CN104054634B CN 104054634 B CN104054634 B CN 104054634B CN 201310290303 A CN201310290303 A CN 201310290303A CN 104054634 B CN104054634 B CN 104054634B
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Abstract
The present invention relates to cultural technique field, disclose a kind of artificial propagation techniques of Semen Caesalpiniae.Step of the present invention is: (1) by Cerambycid beetles according to female: hero=1-8:1 ratio is placed in indoor dependent insect cage cultivates to laying eggs; (2) getting worm's ovum to moving in incubator box after its sterilization, at temperature 18-30 DEG C, under relative moisture 50%-85% environment, hatching into primary larva; (3) primary larva is proceeded to cultivate to elementary foster scolite; (4) when primary larva grows to 3 instar larvae, proceed to immediately in secondary foster scolite and cultivate; Be collected in cultivate in secondary foster scolite 3 age to 5 instar larvae, as medicinal Semen Caesalpiniae.The present invention simulates field natural conditions, achieve and the Semen Caesalpiniae of original natural propagation is in the wild transformed into indoor artificial propagation, growth cycle shortens greatly, and output is large and stable, survival rate is high, Anti-bacterium, resistance infection strong, and product nutrient component and drug effect are equal to substantially with wild Semen Caesalpiniae.
Description
Technical field
The present invention relates to cultural technique field, particularly a kind of artificial propagation techniques of Semen Caesalpiniae.
Background technology
Semen Caesalpiniae is the larva of longicorn parasitic in leguminous plant mysorethorn stem and root and nearly edge insect thereof, has another name called the stupid worm of mysorethorn (Jiangxi " herbal medicine handbook "), hundred crows do not stop moth " Chinese herbal drugs database ", ox thorn worm " the Zhejiang traditional Chinese medical science ", bucket cadelle " Zhejiang medicinal plant will ", yellow small house thorn worm, roundheaded borer " will planted by Tianmu Mountains of Zhejiang Province medicine ", King of Hell sting worm, one's old mother worm, reversal of the natural order of things thorn worm, mysorethorn root and bark, sparrow do not step on thorn worm, bird not place worm.Semen Caesalpiniae is the folk prescription simply of folklore, is also the key medicine simply of pharmaceutical insects
,there is the long-pending promoting eruption that disappears, channels sootheing and network vessel quickening, loose joint, arresting convulsion, control carbuncle furuncle poison, miliaria rash, the effects such as epilepsy.Cure mainly infantile malnutrition, 1. " Hunan medicine will ": " control numb poison to cave in." measles is sent out unhappy thoroughly, arthralgia and myalgia, and property is pungent, sweet, and temperature enters liver, the spleen channel.Gathering and storage of medicinal material: autumn and winter gathers, seeks mysorethorn tree stem or the channel of root, takes with cutter, with or dry airtight storage.Existing Semen Caesalpiniae can only lean on the channel manually seeking mysorethorn tree stem or root in the wild, take with cutter and obtain, collecting season exists significantly seasonal, the dependence obtained by field is large, and get a cestode and will damage more than 10 jin timber, forest resources waste seriously, not only time-consuming but also require great effort, output is extremely restricted, and directly constrains the further exploitation of Semen Caesalpiniae at food and field of medicaments.
Summary of the invention
The object of the invention is to the above-mentioned defect overcoming prior art existence, a kind of artificial propagation techniques of Semen Caesalpiniae is provided, simulation field natural conditions, achieve and the Semen Caesalpiniae of original natural propagation is in the wild transformed into indoor artificial propagation, growth cycle shortens greatly, stable yield, survival rate is high, Anti-bacterium, resistance infection strong, and product nutrient component and drug effect are equal to substantially with wild Semen Caesalpiniae.
The technical solution adopted for the present invention to solve the technical problems is:
An artificial propagation techniques for Semen Caesalpiniae, comprises the steps:
(1) by Cerambycid beetles in female: hero=1-8:1 ratio is placed in indoor dependent insect cage to be cultured to lays eggs, and condition of culture is: temperature 18-30 DEG C, relative moisture 50%-85%, natural lighting, adopts diameter to be that mysorethorn branch that 0.2-2cm is fresh is as feed.
Control Cerambycid beetles is female: hero=1-8:1 ratio, and such egg laying amount is large, preferably female: hero=1-2:1 ratio, obtains best egg laying amount, and the quality of ovum is also better simultaneously.
The longicorn species of the nearly edge such as the preferred longicorn beetle of kind, mulberry longicorn, yellow spotted star longicorn beetle anoplophora glabripennis, batocera horsfieldi, control of Apriona swainsoni, pink neck longicorn of Cerambycid beetles.
Mysorethorn tree is the main host plants that above-mentioned Cerambycid beetles is corresponding, and it is liked at the new diameter sprouted then is that mysorethorn branch (i.e. mysorethorn spray) that 0.2-2cm is fresh gnaws its bast, and Cerambycid beetles seldom gnaws the mysorethorn branch in more than 2 years vegetative period.For the habit that simulation Cerambycid beetles is gnawed in the wild, ensure that diameter is the freshness of the mysorethorn branch that 0.2-2cm is fresh, this mysorethorn branch length controls at 15-30cm, and be comparatively applicable to the space of dependent insect cage and the amount needed for Cerambycid beetles every day, every Cerambycid beetles consumption every day is one.Condition of culture is: temperature 18-30 DEG C, relative moisture 50%-85%, natural lighting, and the environment cultivated like this and field natural environment are close, and are beneficial to Cerambycid beetles and survive and breed.
(2) getting worm's ovum to moving in incubator box after its sterilization, is hatch into primary larva under the environment of 18-30 DEG C, relative moisture 50%-85% in temperature.
Control incubation temperature 18-30 DEG C, relative moisture 50%-85%, is beneficial to egg hatch, improves incubation rate.
(3) proceeded to by the primary larva just hatched and cultivate to elementary foster scolite, condition of culture is: temperature 18-30 DEG C, relative moisture 50%-85%, dark.
The condition of culture simulated and natural conditions more close, survival rate of larvae is high.Cultivate in elementary foster scolite and mainly cultivate 1-2 instar larvae, when growing to 3 instar larvae after 2 instar larvaes are just casted off a skin, be transferred to immediately in secondary foster scolite and cultivate, because when growing to 3 instar larvae, the activity space of elementary foster scolite can not meet 3 age above larva growth and the demand of feed, therefore, need for larva expands activity space and enough feeds.
The larva of Semen Caesalpiniae divides 5 growth and development stages (be 1-5 age) larva, the division in worm age is as the criterion (existing definition) with number of times of casting off a skin, namely primary larva (i.e. 1 instar larvae) casts off a skin after cultivating once becomes 2 instar larvaes, namely 2 instar larvaes are casted off a skin after cultivating once becomes 3 instar larvaes, the like.
(4) when primary larva grows to 3 instar larvae, proceed to immediately in secondary foster scolite and cultivate, condition of culture is: temperature 18-30 DEG C, relative moisture 50%-85%, dark; Be collected in cultivate in secondary foster scolite 3 age to 5 instar larvae, as medicinal or edible Semen Caesalpiniae.
3 instars cultivated in secondary foster scolite are to the incubation time about 30 days of casting off a skin to 4 ages, and namely the larva entering 4 ages and 5 ages becomes mature larva.3 age to 5 instar larvae all can gather in the crops and use as medicinal Semen Caesalpiniae, but its nutrition of the larva just entering for 3 length of times is not also consolidated, and needs to cultivate about 30 days time again in secondary foster scolite, can gather in the crops using fresh goods as medicinal or edible.The best medicinal phase is the mature larva in the 4-5 length of time.In advance or larva washiness of gathering out of season, the product of processing is easily mouldy not easily to be stored, and the enteron aisle of larva is detained wood chip and excreta, affects product sense organ and and unhygienic.
As preferably, in step (1), condition of culture is: temperature 25-27 DEG C, relative moisture 65%-
80%, natural lighting, employing diameter is 0.5-2cm, and length is that the fresh mysorethorn branch of 15-30cm is as feed.
As preferably, in step (2), in incubator box, temperature controls at 25-27 DEG C, relative moisture 65%-
80%.Temperature and humidity of controlling well is conducive to the raising of egg hatch rate.
As preferably, in the elementary foster scolite of every root and secondary foster scolite, all only limit cultivation 1 larva.Because the biological property of roundheaded borer be two larva in same culture tube, will mutually bait lethal, therefore, each culture tube controlled feeding larva.
As preferably, the condition of culture in step (3) and step (4) is: temperature 25-27 DEG C, relative moisture 65%-80%, dark.The environment cultivated and field natural environment are close, and are beneficial to larva normal development and grow up and improve survival rate.
As preferably, elementary foster scolite is the logical glass tube in two, and elementary foster scolite internal diameter is 0.5-1cm, length 8-12cm, the elementary foster worm feed of elementary foster scolite central filler, and two screw plugs are breathed freely material plug, and elementary foster worm feed is changed once for 5-7 days.Elementary foster scolite two leads to, and two screw plugs are breathed freely material plug, and object is that to guarantee to manage interior air-flow unobstructed, prevents larva for want of air-flow and falling ill, even dead.
As preferably, the each component of described elementary foster worm feed by weight its formula is: mysorethorn spray powder 100-250 part, animal oil 0.5-5 part, Vc0.5-5 part, phycocolloid powder 2-20 part, acetic acid 0.5-10 part, antifungal agent 0.1-2 part, Webster salt 0.5-10 part, compound preservative 0.5-8 part, water 1-30 part.
As preferably, secondary foster scolite is the logical glass tube in two, and secondary foster scolite internal diameter is 1.5-3.5cm, length 13-18cm, the secondary foster worm feed of secondary foster scolite central filler, and two screw plugs are breathed freely material plug, and secondary foster worm feed is changed once for 10-15 days.Secondary foster scolite two leads to, and two screw plugs are breathed freely material plug, and object is that to guarantee to manage interior air-flow unobstructed, prevents larva for want of air-flow and falling ill, even dead.
As preferably, each component of described secondary foster worm feed by weight its formula is: mysorethorn old branch powder 300-550 part, animal oil 0.5-5 part, Vc0.5-5 part, phycocolloid powder 4-20 part, acetic acid 1-10 part, antifungal agent 0.2-3 part, Webster salt 4-20 part, compound preservative 0.5-10 part, water 2-40 part.
As preferably, described compound preservative is mixed by 5-10wt% garlic oil, 20-40wt% vinegar and 50-75wt% lactic acid bacteria.Adopt the formula of this compound preservative that the shelf-life of feed is extended greatly, favorable anti-corrosion effect, good palatability, can promote larva appetite, in addition, can also sterilizing, improve resistance against diseases.
According to the feeding habit of field Semen Caesalpiniae young instar after hatching, larger difference is had in the demand of Different growth phases to nutrition in conjunction with larval phase, 1-2 instar larvae is cultivated, the specific nutrition demand for this stage larvae is adopted to provide elementary foster worm feed, 3 age above Larval foods demand continuing to increase, therefore, the specific nutrition demand for this stage larvae is adopted to provide secondary foster worm feed.
Elementary foster worm feed primary raw material is mysorethorn spray powder (30-100 order), mysorethorn spray powder is form after mysorethorn spray is pulverized, mysorethorn spray is for propagating then the new branch (stem) that (namely Fodder making then) mysorethorn grows artificially, and the vegetative period of mysorethorn spray is within 1 year.1-2 instar larvae more tender (digestion power and masticatory ability more weak), food is advisable with mysorethorn spray powder, good palatability, and nutrition easily absorbs, and is not suitable for the old branch powder of mysorethorn and raises.Arrived for 3 above stages in age, when larvae development is comparatively ripe, then need to be replaced by secondary foster scolite and secondary foster worm feed, for larva expands activity space and enough feeds, to meet the demand of its nutritional amt.Secondary foster worm feed primary raw material is the old branch powder of mysorethorn, and mysorethorn old branch powder is the old branch of mysorethorn (stem) gathering more than 1 year vegetative period, forms after pulverizing.
Mysorethorn spray in the present invention, the old branch of mysorethorn all refer to the stem of mysorethorn.
In feed, mysorethorn tender (always) branch powder is natural lignocellulose, is the Major Foods of larval feeding growth; Animal oil provides the desired nutritional materials such as unsaturated fatty acid, crude fat and several amino acids for larva; Vc(ascorbic acid) for feed antistaling agent, promote larva appetite, enhancing development; Phycocolloid powder is the humectant of feed, is beneficial to feed shaping, simultaneously the nutrition of complementary diets fiber and protein; Acetic acid can suppress mould in feed and putrefying bacteria; Antifungal agent is the antibacterial biological mildew inhibitor of feed; Webster salt is used for supplementing its trace element, impels larva normal growth to grow.Due to the mysorethorn branch crushed material that the main component in feed is fresh, perishable and go mouldy, adopt special compound preservative, to feedstuff mildew favorable anti-corrosion effect, strengthen the immunologic function of larva.The prescription feed of the present invention's preparation, ensure that the harmony in feed nutrition collocation and freshness, larva is not easily fallen ill, the Semen Caesalpiniae survival rate of raising is high, Anti-bacterium, resistance infection are strong, and growth cycle shortens greatly, and its nutrient component and drug effect are equal to substantially with wild Semen Caesalpiniae.
The invention has the beneficial effects as follows: simulation field natural conditions, achieve and the Semen Caesalpiniae of original natural propagation is in the wild transformed into indoor artificial propagation, growth cycle shortens greatly, stable yield, survival rate is high, Anti-bacterium, resistance infection strong, and product nutrient component and drug effect are equal to substantially with wild Semen Caesalpiniae.
Embodiment
Below by specific embodiment, technical scheme of the present invention is described in further detail.
In the present invention, if not refer in particular to, the raw material adopted and equipment etc. all can be buied from market or this area is conventional.Method in following embodiment, if no special instructions, is the conventional method of this area.
In June, 2012, Cerambycid beetles is caught in Jinhua suburb field, preferred sky cattle breeds is the longicorns such as longicorn beetle, mulberry longicorn, yellow spotted star longicorn beetle, anoplophora glabripennis, batocera horsfieldi, control of Apriona swainsoni, pink neck longicorn, and can be wherein a kind of, also can be several.
In the present invention, the primary raw material-mysorethorn spray powder of elementary foster worm feed is form after mysorethorn spray is pulverized, and mysorethorn spray is for propagating then the new branch that (namely Fodder making then) mysorethorn grows artificially, and the vegetative period of mysorethorn spray is within 1 year.Make elementary foster worm feed the annual 6-12 month, gather the shoot that sprouts in mysorethorn from January then as mysorethorn spray, after directly mysorethorn spray being pulverized mysorethorn spray powder.
Concrete example is illustrated as: if make elementary foster worm feed in August, 2012, then the shoot sprouted in mysorethorn before gathering in August, 2012, as mysorethorn spray, obtains mysorethorn spray powder after directly pulverizing.
Secondary foster worm feed primary raw material-mysorethorn old branch powder is the old branch of collection above mysorethorn in 2 year vegetative period, forms after directly pulverizing.
Secondary foster worm feed primary raw material-mysorethorn old branch powder is the old branch of the mysorethorn gathering more than 1 year vegetative period, forms after directly pulverizing.
Mysorethorn spray in the present invention, the old branch of mysorethorn all refer to the stem of mysorethorn.
Embodiment 1:
(1) by Cerambycid beetles in female: hero=1:1 ratio is placed in indoor dependent insect cage to be cultured to lays eggs, condition of culture is: temperature 18 DEG C, relative moisture 50%, natural lighting, diameter is adopted to be that mysorethorn branch that 0.2-2cm is fresh (2012 then the new branch sprouted) is as feed, preferred diameter is 0.5-2cm, concrete enforcement can adopt diameter to be about 1cm, this mysorethorn branch length controls at 15-30cm, concrete enforcement can adopt about length 20cm, comparatively be applicable to the space of dependent insect cage and the amount needed for Cerambycid beetles every day, every Cerambycid beetles consumption every day is one.
(2) getting worm's ovum to moving in incubator box after its sterilization, being 18 DEG C in temperature, under the environment of relative moisture 50%, hatching into primary larva.
(3) proceeded to by primary larva and cultivate to elementary foster scolite, condition of culture is: temperature 18 DEG C, relative moisture 50%, dark.
Elementary foster scolite is the logical glass tube in two, and elementary foster scolite internal diameter is 0.5cm, length 8cm, the elementary foster worm feed of elementary foster scolite central filler, and two screw plugs are breathed freely material plug, and elementary foster worm feed is changed once for 5 days.
(4) when primary larva grows to 3 instar larvae, proceed to immediately in secondary foster scolite and cultivate, condition of culture is: temperature 18 DEG C, relative moisture 50%, dark; Be collected in secondary foster scolite and be cultured to 5 instar larvaes, as medicinal Semen Caesalpiniae.
Secondary foster scolite is the logical glass tube in two, and secondary foster scolite internal diameter is 1.5cm, length 13cm, the secondary foster worm feed of secondary foster scolite central filler, and two screw plugs are breathed freely material plug, and secondary foster worm feed is changed once for 10 days.
The each component of elementary foster worm feed by weight its formula is: 100 parts, mysorethorn spray powder, 0.5 part, animal oil (commercially available), Vc0.5 part, 2 parts, phycocolloid powder (commercially available), mass concentration is the acetic acid 0.5 part of 30%, antifungal agent (natamycin, commercially available) 0.1 part, Webster salt (commercially available) 0.5 part, compound preservative 0.5 part, 1 part, water;
The each component of secondary foster worm feed by weight its formula is: 300 parts, the old branch powder of mysorethorn, 0.5 part, animal oil, Vc0.5 part, 4 parts, phycocolloid powder (commercially available), mass concentration is the acetic acid 1 part of 30%, antifungal agent (natamycin, commercially available) 0.2 part, Webster salt (commercially available) 4 parts, compound preservative 0.5 part, 2 parts, water.
The fineness of mysorethorn spray powder and the old branch powder of mysorethorn is 30-100 order, and preferred fineness is 40-80 order.
Compound preservative is mixed by 5wt% garlic oil (commercially available), 20wt% vinegar (commercially available) and 75wt% lactic acid bacteria (commercially available).
Embodiment 2:
(1) by Cerambycid beetles in female: hero=8:1 ratio is placed in indoor dependent insect cage to be cultured to lays eggs, condition of culture is: temperature 30 DEG C, relative moisture 85%, natural lighting, diameter is adopted to be that mysorethorn branch that 0.2-2cm is fresh (2012 then the new branch sprouted) is as feed, preferred diameter is 0.5-2cm, concrete enforcement can adopt diameter to be about 1.5cm, this mysorethorn branch length controls at 15-30cm, concrete enforcement can adopt about length 25cm, comparatively be applicable to the space of dependent insect cage and the amount needed for Cerambycid beetles every day, every Cerambycid beetles consumption every day is one.
(2) getting worm's ovum to moving in incubator box after its sterilization, being 30 DEG C in temperature, under the environment of relative moisture 85%, hatching into primary larva.
(3) proceeded to by primary larva and cultivate to elementary foster scolite, condition of culture is: temperature 30 DEG C, relative moisture 85%, dark.
Elementary foster scolite is the logical glass tube in two, and elementary foster scolite internal diameter is 1cm, length 12cm, the elementary foster worm feed of elementary foster scolite central filler, and two screw plugs are breathed freely material plug, and elementary foster worm feed is changed once for 7 days.
(4) when primary larva grows to 3 instar larvae, proceed to immediately in secondary foster scolite and cultivate, condition of culture is: temperature 30 DEG C, relative moisture 85%, dark; Be collected in 3 instar larvaes that the consolidation of cultivating in secondary foster scolite is cultivated 30 days, as edible Semen Caesalpiniae.
Secondary foster scolite is the logical glass tube in two, and secondary foster scolite internal diameter is 3.5cm, length 18cm, the secondary foster worm feed of secondary foster scolite central filler, and two screw plugs are breathed freely material plug, and secondary foster worm feed is changed once for 15 days.
The each component of described elementary foster worm feed by weight its formula is: 250 parts, mysorethorn spray powder, 5 parts, animal oil, Vc5 part, 20 parts, phycocolloid powder, and mass concentration is the acetic acid 10 parts of 5%, antifungal agent 2 parts, Webster salt 10 parts, compound preservative 8 parts, 30 parts, water;
The each component of described secondary foster worm feed by weight its formula is: 550 parts, the old branch powder of mysorethorn, 5 parts, animal oil, Vc5 part, 20 parts, phycocolloid powder, and mass concentration is the acetic acid 10 parts of 5%, antifungal agent 3 parts, Webster salt 20 parts, compound preservative 10 parts, 40 parts, water.
The fineness of mysorethorn spray powder and the old branch powder of mysorethorn is 30-100 order, and preferred fineness is 40-80 order.
Compound preservative is mixed by 10wt% garlic oil, 40wt% vinegar and 50wt% lactic acid bacteria.
Embodiment 3:
(1) by Cerambycid beetles in female: hero=2:1 ratio is placed in indoor dependent insect cage to be cultured to lays eggs, condition of culture is: temperature 25 DEG C, relative moisture 65%, natural lighting, diameter is adopted to be that mysorethorn branch that 0.2-2cm is fresh (2012 then the new branch sprouted) is as feed, preferred diameter is 0.5-2cm, concrete enforcement can adopt diameter to be about 1cm, this mysorethorn branch length controls at 15-30cm, concrete enforcement can adopt length 20cm, comparatively be applicable to the space of dependent insect cage and the amount needed for Cerambycid beetles every day, every Cerambycid beetles consumption every day is one.
(2) getting worm's ovum to moving in incubator box after its sterilization, being 25 DEG C in temperature, under the environment of relative moisture 65%, hatching into primary larva.
(3) proceeded to by primary larva and cultivate to elementary foster scolite, condition of culture is: temperature 25 DEG C, relative moisture 65%, dark.
Elementary foster scolite is the logical glass tube in two, and elementary foster scolite internal diameter is 0.8cm, length 10cm, the elementary foster worm feed of elementary foster scolite central filler, and two screw plugs are breathed freely material plug, and elementary foster worm feed is changed once for 6 days.
(4) when primary larva grows to 3 instar larvae, proceed to immediately in secondary foster scolite and cultivate, condition of culture is: temperature 25 DEG C, relative moisture 65%
,dark; Be collected in secondary foster scolite and be cultured to 4 instar larvaes, as edible Semen Caesalpiniae.
Secondary foster scolite is the logical glass tube in two, and secondary foster scolite internal diameter is 2cm, length 15cm, the secondary foster worm feed of secondary foster scolite central filler, and two screw plugs are breathed freely material plug, and secondary foster worm feed is changed once for 12 days.
The each component of described elementary foster worm feed by weight its formula is: 200 parts, mysorethorn spray powder, 1 part, animal oil, Vc1 part, 15 parts, phycocolloid powder, and mass concentration is the acetic acid 5 parts of 10%, antifungal agent 0.1 part, Webster salt 7.5 parts, compound preservative 1 part, 10 parts, water;
The each component of described secondary foster worm feed by weight its formula is: 400 parts, the old branch powder of mysorethorn, 2 parts, animal oil, Vc2 part, 15 parts, phycocolloid powder, and mass concentration is the acetic acid 5 parts of 10%, antifungal agent 1 part, Webster salt 15 parts, compound preservative 2 parts, 25 parts, water.
The fineness of mysorethorn spray powder and the old branch powder of mysorethorn is 30-100 order, and preferred fineness is 40-80 order.
Compound preservative is mixed by 8wt% garlic oil, 30wt% vinegar and 62wt% lactic acid bacteria.
Embodiment 4:
(1) by Cerambycid beetles in female: hero=2:1 ratio is placed in indoor dependent insect cage to be cultured to lays eggs, condition of culture is: temperature 27 DEG C, relative moisture 80%, natural lighting, diameter is adopted to be that mysorethorn branch that 0.2-2cm is fresh (2012 then the new branch sprouted) is as feed, preferred diameter is 0.5-2cm, concrete enforcement can adopt diameter to be about 1cm, this mysorethorn branch length controls at 15-30cm, concrete enforcement can adopt length 20cm, comparatively be applicable to the space of dependent insect cage and the amount needed for Cerambycid beetles every day, every Cerambycid beetles consumption every day is one.
(2) getting worm's ovum to moving in incubator box after its sterilization, being 27 DEG C in temperature, under the environment of relative moisture 80%, hatching into primary larva.
(3) proceeded to by primary larva and cultivate to elementary foster scolite, condition of culture is: temperature 27 DEG C, relative moisture 80%, dark.
Elementary foster scolite is the logical glass tube in two, and elementary foster scolite internal diameter is 0.9cm, length 10cm, the elementary foster worm feed of elementary foster scolite central filler, and two screw plugs are breathed freely material plug, and elementary foster worm feed is changed once for 6 days.
(4) when primary larva grows to 3 instar larvae, proceed to immediately in secondary foster scolite and cultivate, condition of culture is: temperature 27 DEG C, relative moisture 80%, dark; Be collected in secondary foster scolite and be cultured to 4 instar larvaes, as medicinal Semen Caesalpiniae.
Secondary foster scolite is the logical glass tube in two, and secondary foster scolite internal diameter is 2.5cm, length 15cm, the secondary foster worm feed of secondary foster scolite central filler, and two screw plugs are breathed freely material plug, and secondary foster worm feed is changed once for 13 days.
The each component of described elementary foster worm feed by weight its formula is: 200 parts, mysorethorn spray powder, 1 part, animal oil, Vc1 part, 15 parts, phycocolloid powder, and mass concentration is the acetic acid 5 parts of 10%, antifungal agent 0.1 part, Webster salt 7.5 parts, compound preservative 1 part, 10 parts, water;
The each component of described secondary foster worm feed by weight its formula is: 400 parts, the old branch powder of mysorethorn, 2 parts, animal oil, Vc2 part, 15 parts, phycocolloid powder, and mass concentration is the acetic acid 5 parts of 10%, antifungal agent 1 part, Webster salt 15 parts, compound preservative 2 parts, 25 parts, water.
The fineness of mysorethorn spray powder and the old branch powder of mysorethorn is 30-100 order, and preferred fineness is 40-80 order.
Compound preservative is mixed by 8wt% garlic oil, 30wt% vinegar and 62wt% lactic acid bacteria.
Feed tubulature method of the present invention: the end opening of supporting scolite (elementary foster scolite, secondary foster scolite) with ventilative material plug (specifically can select cotton balls) jam-pack, at filling suitable for reading prescription feed of the present invention, when being filled to pipe range 2/3rds, again by the access of the larva of taking-up wherein, continue to fill when feed is extremely located from 1 centimeter suitable for reading by ventilative material plug beyond the Great Wall suitable for reading.
The present invention catches Cerambycid beetles in the annual 5-7 month from field, is put in dependent insect cage, and raise through cultivating, some same day lay eggs, and what have then needs within 3-5 days, lay eggs, hatching process 12-15 days.1-2 incubation time in age about 2 months, consolidates cultivation 3 ages and namely can be used as product in 30 days, from 3 ages cultivate 5 age incubation time be about 3-4 month.The whole artificial propagation cycle is about 5-6 month, then needs more than 10 months under the natural conditions of field, and growth cycle shortens 4-5 month, survival rate more than 80% of the present invention, infection rate less than 5%
,stable yield, survival rate is high, and product nutrient component and drug effect are equal to substantially with wild Semen Caesalpiniae.
The present invention is in enforcement and operation, it is no matter the sterilization of environment, worm's ovum and feed, or feed formula all adopts the method for modern biotechnology mildew-resistant, and the overall process of artificial propagation does not adopt any chemical agent, the Semen Caesalpiniae product of breeding for food or medicine all fool proof.
Above-described embodiment is one of the present invention preferably scheme, not does any pro forma restriction to the present invention, also has other variant and remodeling under the prerequisite not exceeding the technical scheme described in claim.
Claims (9)
1. an artificial fecundation method for Semen Caesalpiniae, is characterized in that: comprise the steps:
(1) by Cerambycid beetles in female: hero=1-8:1 ratio is placed in indoor dependent insect cage to be cultured to lays eggs, and condition of culture is: temperature 18-30 DEG C, relative moisture 50%-85%, natural lighting, adopts diameter to be that mysorethorn branch that 0.2-2cm is fresh is as feed;
(2) getting worm's ovum to moving in incubator box after its sterilization, is hatch into primary larva under the environment of 18-30 DEG C, relative moisture 50%-85% in temperature;
(3) proceeded to by the primary larva just hatched and cultivate to elementary foster scolite, condition of culture is: temperature 18-30 DEG C, relative moisture 50%-80%, dark;
(4) when primary larva grows to 3 instar larvae, proceed to immediately in secondary foster scolite and cultivate, condition of culture is: temperature 18-30 DEG C, relative moisture 50%-85%, dark; Be collected in cultivate in secondary foster scolite 3 age to 5 instar larvae, namely as medicinal or edible Semen Caesalpiniae; Secondary foster scolite is the logical glass tube in two, and secondary foster scolite internal diameter is 1.5-3.5cm, length 13-18cm, the secondary foster worm feed of secondary foster scolite central filler, and two screw plugs are breathed freely material plug, and secondary foster worm feed is changed once for 10-15 days.
2. artificial fecundation method according to claim 1, is characterized in that: in step (1), condition of culture is: temperature 25-27 DEG C, relative moisture 65%-80%, natural lighting, employing diameter is 0.5-2cm, and length is that the fresh mysorethorn branch of 15-30cm is as feed.
3. artificial fecundation method according to claim 1, is characterized in that: in step (2), in incubator box, temperature controls at 25-27 DEG C, relative moisture 65%-80%.
4. the artificial fecundation method according to claim 1 or 2 or 3, is characterized in that: all only limit cultivation 1 larva in every elementary foster scolite of root and secondary foster scolite.
5. the artificial fecundation method according to claim 1 or 2 or 3, is characterized in that: the condition of culture in step (3) and step (4) is: temperature 25-27 DEG C, relative moisture 65%-80%, dark.
6. artificial fecundation method according to claim 1, it is characterized in that: elementary foster scolite is the glass tube that two leads to, elementary foster scolite internal diameter is 0.5-1cm, length 8-12cm, the elementary foster worm feed of elementary foster scolite central filler, two screw plugs are breathed freely material plug, and elementary foster worm feed is changed once for 5-7 days.
7. artificial fecundation method according to claim 6, it is characterized in that: each component of described elementary foster worm feed by weight its formula is: mysorethorn spray powder 100-250 part, animal oil 0.5-5 part, Vc0.5-5 part, phycocolloid powder 2-20 part, acetic acid 0.5-10 part, antifungal agent 0.1-2 part, Webster salt 0.5-10 part, compound preservative 0.5-8 part, water 1-30 part; Mysorethorn spray powder is form after mysorethorn spray is pulverized, and mysorethorn spray is propagate the new branch that mysorethorn then grows artificially, and the vegetative period of mysorethorn spray is within 1 year.
8. artificial fecundation method according to claim 1, it is characterized in that: each component of described secondary foster worm feed by weight its formula is: mysorethorn old branch powder 300-550 part, animal oil 0.5-5 part, Vc0.5-5 part, phycocolloid powder 4-20 part, acetic acid 1-10 part, antifungal agent 0.2-3 part, Webster salt 4-20 part, compound preservative 0.5-10 part, water 2-40 part; Mysorethorn old branch powder gathers the old branch of mysorethorn on 1 year vegetative period, forms after pulverizing.
9. the artificial fecundation method according to claim 7 or 8, is characterized in that: described compound preservative is mixed by 5-10wt% garlic oil, 20-40wt% vinegar and 50-75wt% lactic acid bacteria.
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Effective date of registration: 20220802 Address after: 321052 No. 29, Youdian street, andI village, andI Town, Wucheng District, Jinhua City, Zhejiang Province Patentee after: Zhejiang forest treasure agriculture and Forestry Technology Development Co.,Ltd. Address before: 321000 No. 93, Zhenger village, andI Town, Wucheng District, Jinhua City, Zhejiang Province Patentee before: Ye Weibang |