CN103843724B - A kind of artificial group breeding method of prodenia litura - Google Patents
A kind of artificial group breeding method of prodenia litura Download PDFInfo
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- CN103843724B CN103843724B CN201410036275.0A CN201410036275A CN103843724B CN 103843724 B CN103843724 B CN 103843724B CN 201410036275 A CN201410036275 A CN 201410036275A CN 103843724 B CN103843724 B CN 103843724B
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Abstract
The invention discloses a kind of prodenia litura Prodenia litura(Fabricius) artificial group breeding method.Larva and appropriate feed are put into diameter and be respectively raising through the plastic casing of sterilizing of 11.5cm, 13.5cm and 16.5cm, cover gauze, cover tightly (1 ~ 3 instar larvae) with lid or pinion box mouth (more than 4 ages) with rubber band, change feed and plastic casing every day, choose from feed after it is pupated, put into plastic casing, absorbent cotton moisturizing; Glass is put in adult male and female pairing after sprouting wings, allows it breed, under certain rearing conditions, form experimental population.This method is simple and convenient, and feeding cost is low, and feeding effect is good, can at indoor continuously rearing.
Description
Technical field
The present invention relates to the group breeding method of the raising, particularly a kind of prodenia litura of agriculture melon dish insect.
Background technology
Prodenia litura Prodenia litura(Fabricius) belong to Lepidoptera (Lepidoptera), Noctuidae (Noctuidae), be global polyphagy, migratory pest.Mainly cause harm with larva, the gluttony phase is entered after 4 ages in days, the light then blade of the plant that is injured is eaten into be incised, hole or netted, heavy then only surplus vein, more severe patient even endangers cane, causes total crop failure, and the worm excrement of discharge pollutes melon dish, insect bite wound easily brings out the diseases such as the soft corruption of fruits and vegetables, epidemic disease, virus disease, has a strong impact on the quality and yield of crop.In recent years, along with the change of China's crop mix, add climate warming, prodenia litura occurring and damage increases the weight of year by year, rises to rapidly the normal property sent out, fulminant agriculture major pest by the intermittence in past, sporadic, secondary property insect.
For effectively controlling causing harm of prodenia litura, launching further investigation to the biology of prodenia litura, ecology and Efficacy of Pesticide etc. is a basic job, particularly prevents and treats in the work such as the screening of prodenia litura effective agent, the toxicologic study of pharmacy effect and raised growth must be had to grow consistent examination worm.Given this, how to obtain a large amount of examination worm by artificial feeding and just become one of important step of prodenia litura research.About the research of prodenia litura rearing method, no matter (2000) such as Zhu Limei or be coated with the careless and Zeng Jihua (2010) of industry, Huang Yanjun and Pu be preced with that diligent (2011) report about in prodenia litura artificial diet technique, all dactylethrae will be used, dependent insect cage or culture dish, plastics ten orifice plate even glass jar, the complicated irregular utensil of jam jar etc., and (2013) such as Lv Baoqian apply for about the patent of raising prodenia litura in also used Tissue Culture Plate and carried out single head raising, even comprise the feed prepared directly to pour in Tissue Culture Plate in its process and cool, this course work amount is large and easily poured out by feed and each insect appetite is different, be easy to the waste causing feed.
Prodenia litura there is no report autotomy habit, and single head raising prodenia litura survival rate is low, workload large, cost is higher and the cleaning of apparatus is also cumbersome, so group breeding is best suited for the method for raising prodenia litura.Can carry out feed replacing according to actual conditions in the process of breeding grub every day, save feed, and raise easy cleaning, the sterilization such as plastic casing, gauze of prodenia litura, workload is less, can reuse and also can greatly reduce costs.
Summary of the invention
For the problem lacking enough standardization examination worm in the screening of prodenia litura pesticide control and pesticide toxicology research and the needs carried out prodenia litura biology, ecological further investigation, the object of the invention is to, a kind of prodenia litura indoors artificial group breeding method is provided.
In order to realize above-mentioned task, the present invention takes following technical solution:
An artificial group breeding method for prodenia litura, is characterized in that, the method adopts artificial feed to raise prodenia litura, comprises the following steps:
A, first configuration prodenia litura artificial feed, sealing refrigeration in 4 DEG C of refrigerators, ready access upon use;
Described artificial feed formula is: corn flour: 200g, soybean meal: 100g, dusty yeast: 12g, ascorbic acid: 6g, sorbic acid: 2g, methyl hydroxybenzoate: 2g, agar: 15g, composite vitamin liquid 15ml, sucrose 50g, water 1000g.
B, ovum are to newly hatched larvae: be placed in the plastic casing of sterilization by prodenia litura ovum and a small amount of feed, be covered with gauze, cover tightly lid, it is 27 ± 1 DEG C in temperature, relative moisture 75 ± 10%, photoperiod 16:8(L:D) h, intensity of illumination 8250lux condition under raise;
C, newly hatched larvae are to pupa: appropriate artificial feed and newly hatched larvae are put into the plastic casing of having sterilized, and the climatic cabinate continuing to put into above-mentioned temp. and humidity and illumination condition is raised, and the actual conditions according to feed and larva regularly replace feed and plastic casing;
D, pupa are to adult: after larvae pupation, are chosen by pupa from artificial feed, put into plastic casing, the built-in wet cotton balls moisturizing of box;
E, adult are to ovum: after pupa turns into adult, emerging adult male and female are mixed, a 1000ml beaker is put into female-male proportion 1:3 or 3:1, placing paper of laying eggs in cup is that the cotton balls of the hydromel of 10% is as Adult supplement nutrient source with being moistened with concentration, every day adds hydromel in cotton balls, changes paper of laying eggs every other day.
The artificial group breeding method of prodenia litura of the present invention is simple and convenient, and feeding cost is low, and feeding effect is good, at indoor continuously rearing, can obtain the prodenia litura worm source of enough growing consistent.
Embodiment
In order to clearly understand the present invention, below in conjunction with embodiment, the present invention is further described in detail.
According to the artificial group breeding method of prodenia litura of the present invention, specifically comprise the steps:
1, prepare prodenia litura artificial feed, its formula is see table 1.
Table 1: Spodoptera litura larvae artificial feed are filled a prescription
| Composition (Ingredient) | Consumption (Dosage) (g) | Source (Source) |
| Corn flour (Maize powder) | 200 | Commercial |
| Soybean meal (Soybean powder) | 100 | Commercial |
| Dusty yeast (Yeast powder) | 12 | Angel Yeast Co., Ltd |
| Ascorbic acid L-ascorbic acid | 6 | Xi'an chemical reagent factory |
| Sorbic acid (Sorbic acid) | 2 | Tianjin Bo Di Chemical Co., Ltd. |
| Methyl hydroxybenzoate (Nipagin ester) | 2 | Tianjin Deng Feng chemical reagent factory |
| Agar (Agar) | 15 | The south of Fujian Province agar-agar Co., Ltd |
| Distilled water (De-ionized water) | 1000 | Laboratory makes by oneself |
| Composite vitamin liquid (Vitamins liquid) | 15ml | Laboratory's autogamy |
| Sucrose (Saccharose) | 50g | Commercial |
Concrete prodenia litura artificial feed preparation:
First by the composite vitamin liquid of formulated, its compound method is, in every 500ml water, add following raw material:
Nicotinic acid (V
b5): 1.528g, D-VB5 calcium (V
b3): 1.525g, vitamin b3: 0.764g, vitamin: B1(V
b1): 0.382g, vitamin B6 (V
b6): 0.382g, folic acid 0.382g, Bio 0.305g, cyanocobalamin: 0.003g, gently stir by hand, put into 4 DEG C of refrigerators for subsequent use after each raw material dissolves;
By 200g corn flour, 100g soybean meal, 12g dusty yeast mixing after put into pot steam 30min after stand-by; Then 15g agar and 50g sucrose being added 1000ml distilled water boils until dissolve completely, 2g sorbic acid, 2g methyl hydroxybenzoate, 6g ascorbic acid and 15ml composite vitamin liquid is added when being cooled to about 50 DEG C, stir until dissolve, the last mixture adding steamed corn flour, soybean meal and dusty yeast wherein, crisper (the diameter 18cm after the clorox soaking disinfection 12h having used concentration 0.2% is poured into after stirring, height 10cm) in, to be cooledly to seal to room temperature, be placed in the refrigeration of 4 DEG C, refrigerator, ready access upon use.
2, ovum is to newly hatched larvae: prodenia litura ovum and a small amount of feed are placed on through 75% the plastic casing of ethanol disinfection (diameter is 16.5cm, high 9cm) in, it is 27 ± 1 DEG C in temperature, relative moisture 75 ± 10%, photoperiod 16:8(L:D) h, raise under the condition of intensity of illumination 8250lux, treat that it is hatched;
3, newly hatched larvae is to pupa: appropriate artificial feed and newly hatched larvae being put into through over-richness is that (diameter is respectively 11.5cm, 13.5cm, 16.5cm for the plastic casing of the ethanol disinfection of 75%, highly be respectively 6.5cm, 7.5cm, 9cm), 1 ~ 2 day old larva is placed on (700 ~ 1500, every box) in the plastic casing of diameter 16.5cm and raises, be covered with gauze, cover tightly lid and prevent larva from runing away; 3 ~ 4 day old larva can be placed on diameter and be respectively in the plastic casing (80 ± 10, every box) of the plastic casing (30 ± 10, every box) of 11.5cm, the plastic casing (50 ± 10, every box) of 13.5cm, 16.5cm and raise, 5 ~ 6 day old larva can be placed on diameter and be respectively in the plastic casing (20 ± 5, every box) of the plastic casing (10 ± 5, every box) of 11.5cm, the plastic casing (15 ± 5, every box) of 13.5cm, 16.5cm and raise, be covered with gauze, in order to prevent, box humidity is excessive easily causes larva to catch, and seals plastic casing mouth replace lid with rubber band; The climatic cabinate continuing to put into above-mentioned temp. and humidity and illumination condition is raised, and every day regularly replaces feed and plastic casing according to the actual conditions of feed and larva;
4, pupa is to adult: after larvae pupation, is chosen by pupa from artificial feed, and putting into through over-richness is the plastic casing (diameter is that 13.5cm height is for 7.5cm) of the ethanol disinfection of 75%, the built-in wet cotton balls moisturizing of plastic casing;
5, adult is to ovum: after pupa turns into adult, emerging adult is closed the open plastic tube of a bite by a short a bite draw, male and female mix, a 1000ml beaker is put into female-male proportion 1:3 or 3:1, in cup, placement is laid eggs, and (32 open paper, folding make it produce fold to be more conducive to lay eggs) and to be moistened with content be concentration be the cotton balls of 10% hydromel as Adult supplement nutrient source, in cotton balls, add concentration every day is 10% hydromel, and replacing is every other day laid eggs paper.
6, the qualification of feeding effect: applicant uses method of the present invention to raise prodenia litura continuously in indoor, its larva goes through the phase (14.69 ± 0.3d); Pupa time (9.06 ± 0.03d); Larval phase survival rate average out to 96.28%; Pupa eclosion rate average out to 92.86%; Female and male ratio (0.91 ± 0.006); Single female egg laying amount on average reaches 895.Compared to existing artificial single head breeding technology (Zhu Limei etc., 2000; Huang Yanjun and Pu hat duty, 2011; Be coated with industry illicit sexual relations Zeng Jihua, 2010; Lv Baoqian etc., 2013), feeding effect of the present invention is best, and feeding process saves feed, utensil easy cleaning is sterilized, cost is lower.
Claims (2)
1. an artificial group breeding method for prodenia litura, is characterized in that, the method adopts artificial feed to raise prodenia litura, comprises the following steps:
A, first configuration prodenia litura artificial feed, sealing refrigeration in 4 DEG C of refrigerators, ready access upon use;
Described artificial feed formula is: corn flour: 200g, soybean meal: 100g, dusty yeast: 12g, ascorbic acid: 6g, sorbic acid: 2g, methyl hydroxybenzoate: 2g, agar: 15g, composite vitamin liquid 15ml, sucrose 50g, water 1000g;
B, ovum are to newly hatched larvae: prodenia litura ovum and feed are placed in the plastic casing of sterilization, are covered with gauze, cover tightly lid, it is 27 DEG C ± 1 DEG C in temperature, relative moisture 75 ± 10%, photoperiod 16:8 (L:D) h, raises under the condition of intensity of illumination 8250lux;
C, newly hatched larvae are to pupa: artificial feed and newly hatched larvae are put into the plastic casing of having sterilized, and the climatic cabinate continuing to put into above-mentioned temp. and humidity and illumination condition is raised, and the actual conditions according to feed and larva regularly replace feed and plastic casing;
D, pupa are to adult: after larvae pupation, are chosen by pupa from artificial feed, put into plastic casing, the built-in wet cotton balls moisturizing of box;
E, adult are to ovum: after pupa turns into adult, emerging adult male and female are mixed, a 1000ml beaker is put into female-male proportion 1:3 or 3:1, placing paper of laying eggs in cup is that the cotton balls of the hydromel of 10% is as Adult supplement nutrient source with being moistened with concentration, every day adds hydromel in cotton balls, changes paper of laying eggs every other day;
1 ~ 2 day old larva is placed on diameter 16.5cm, and raise in the plastic casing of height 9cm, 700 ~ 1500, every box, is covered with gauze, covers tightly lid and prevents larva from runing away;
It is 11.5cm that 3 ~ 4 day old larva are placed on diameter, and raise in the plastic casing of height 6.5cm, 30 ± 10, every box, is covered with gauze; Or being placed on diameter is 13.5cm, raise in the plastic casing of height 7.5cm, 50 ± 10, every box, is covered with gauze; Or being placed on diameter is 16.5cm, raise in the plastic casing of height 9cm, 80 ± 10, every box, is covered with gauze; In order to prevent, plastic casing humidity is excessive easily causes larva to catch, and seals plastic casing mouth replace lid with rubber band;
It is 11.5cm that 5 ~ 6 day old larva are placed on diameter, and raise in the plastic casing of height 6.5cm, 10 ± 5, every box, is covered with gauze; Or being placed on diameter is 13.5cm, raise in the plastic casing of height 7.5cm, 15 ± 5, every box, is covered with gauze; Or being placed on diameter is 16.5cm, raise in the plastic casing of height 9cm, 20 ± 5, every box, is covered with gauze; In order to prevent, plastic casing humidity is excessive easily causes larva to catch, and seals plastic casing mouth replace lid with rubber band;
Pupa time worm to be placed on diameter be 13.5cm, in the plastic casing of height 7.5cm, 25 ~ 40, every box, rayon balls moisturizing.
2. the method for claim 1, is characterized in that, the compound method of described composite vitamin liquid is: in every 500ml water, add following raw material mix:
Nicotinic acid: 1.528g, D-VB5 calcium: 1.525g, vitamin b3: 0.764g, vitamin B1: 0.382g, vitamin B6: 0.382g, folic acid: 0.382g, Bio: 0.305g, cyanocobalamin: 0.003g.
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Cited By (1)
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| CN106616070A (en) * | 2016-09-19 | 2017-05-10 | 云南农业大学 | Chestnut weevil artificial feed, cultivation method and application of mature larvae |
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| CN104957398A (en) * | 2015-06-08 | 2015-10-07 | 云南大学 | Artificial feed for spodoptera litura larvae and raising method of spodoptera litura larvae |
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| CN108077191A (en) * | 2017-12-29 | 2018-05-29 | 四川省农业科学院植物保护研究所 | A kind of Spodoptera litura larvae group breeding method |
| CN109644950B (en) * | 2019-02-11 | 2021-11-26 | 广东省烟草南雄科学研究所 | Large-scale breeding method for red-color lygus lucorum |
| CN110089642A (en) * | 2019-06-15 | 2019-08-06 | 安徽省农业科学院植物保护与农产品质量安全研究所 | A kind of Spodopterafrugiperda man-made feeds |
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