CN107683828B - Self-disabled insect feeding and egg collecting method - Google Patents
Self-disabled insect feeding and egg collecting method Download PDFInfo
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- CN107683828B CN107683828B CN201710925934.XA CN201710925934A CN107683828B CN 107683828 B CN107683828 B CN 107683828B CN 201710925934 A CN201710925934 A CN 201710925934A CN 107683828 B CN107683828 B CN 107683828B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
Abstract
The invention discloses a method for breeding and collecting eggs of self-disabled insects, which comprises the steps of firstly manufacturing a breeding box, wherein the breeding box is provided with a plastic transparent box body, the upper part of the plastic transparent box body is large, the lower part of the plastic transparent box body is small, a ventilation window is formed in the side surface of the box body, 1 sponge block is arranged in the plastic transparent box body, and a folded wave-shaped folded paper strip with the length of 200mm and the height of 20mm is arranged in the plastic transparent box body along the inner wall of the box.
Description
Technical Field
The invention relates to a method for feeding and collecting eggs of self-disabled insects.
Background
The harmonia axyridis as an excellent pest prey has the advantages of wide predation range, large predation quantity, long service life, strong adaptability and the like, and has wide prospect in the biological control market of pests. The breeding and propagation of natural enemies are the key points of biological control, and scientific workers also carry out a great deal of research on the mass propagation of the harmonia axyridis and artificial feed. Not only is artificial feed the key point, but the feeding method and egg collection are both the keys of natural enemy feeding. The predatory natural enemies mostly have the habit of killing the natural enemies by self, the feeding quantity in a fixed area is limited, and the breeding of the harmonia axyridis in the net cage wastes space, is not easy to avoid killing the natural enemies by self and is more inconvenient for egg collection. Therefore, it is necessary to create a feeding method which is easy to master and can obtain a large number of natural enemies per unit area, and it is the key to realize commercialization of the natural enemies to explore a simple and convenient method for collecting eggs of the natural enemies.
Disclosure of Invention
The invention aims to provide a self-disabled insect feeding and egg collecting method, which is realized by adopting the following technical scheme:
in order to carry out the self-disabled insect feeding and egg-picking method of the invention, the feeding box is made as follows:
the breeding box is provided with a plastic transparent box body, the plastic transparent box body is in a hollow round or oval shape, the plastic transparent box body is large at the top and small at the bottom, the upper opening of the plastic box body is 75mm in diameter, the lower bottom is 55mm in diameter and 38mm in height, the volume is about 100ml, an opening of 20mm multiplied by 20mm is formed in the side face of the plastic transparent box body, a 100-mesh gauze is sealed on the opening to serve as a ventilation window, 1 sponge block of 10mm multiplied by 10mm is arranged in the plastic transparent box body, and can be used for providing water or honey water after being soaked;
for covering the plastic transparent box body, two covers are respectively used corresponding to the larval stage and the adult stage:
one type is a plastic box cover corresponding to the size of a box opening of a plastic transparent box body, the plastic box cover is provided with a circular or oval buckle groove corresponding to the circular or oval box opening of the plastic transparent box body, and the plastic box cover is used for buckling the box opening of the plastic transparent box body in a larva breeding period;
the other type is a plastic culture dish cover, a plurality of small vent holes penetrating through the box cover are formed in the plastic culture dish cover along the inner periphery of the box cover, the diameter of each small vent hole is about 1.0mm to 2.2mm, and the plastic culture dish cover is replaced by the plastic culture dish cover in the adult stage to cover the box body.
The self-disabled insect breeding and egg collecting method comprises the steps of intensively breeding a small number of harmonia axyridis larvae, collecting the pupated harmonia axyridis larvae for centralized emergence, breeding the pupated harmonia axyridis in a single pair, and collecting eggs, and comprises the following specific steps:
the larva breeding method comprises the following steps: putting 7-8 eggs of the harmonia axyridis to be hatched into a feeding box, adding sufficient amount of eggs of the mediterranean pink borer as food, soaking a sponge block, putting the soaked sponge block into the feeding box, putting the soaked sponge block into a corrugated paper folding strip which is 200mm long and 20mm high, and buckling a box cover to form an independent feeding space, wherein a plurality of feeding boxes can be stacked, and the food is put once every 2 days, and is put 3 times in total, and 0.1g, 0.2g and 0.3g are respectively put in the feeding boxes;
the collection and eclosion method of the pupa comprises the following steps: opening the breeding box after the harmonia axyridis pupates, and cutting off the paper slips where the pupae are located when the pupae are pupated on the paper slips; if pupa is formed on the box wall or the box cover, the pupa is carefully shoveled down by a thin small iron sheet, and the pupa is intensively collected and then is intensively feathered in a large box;
a method for breeding and collecting eggs of harmonia axyridis adults comprises the steps of judging sexes according to genitals after the harmonia axyridis adults emerge for 2 days, pairing, breeding 1 female and 1 male in a single pair, putting a pair of harmonia axyridis into a breeding box, putting eggs of mediterranean pink borers as food, soaking sponge blocks, putting paper folding type paper strips, buckling a box cover, feeding for 1 time every 2 days before spawning, starting spawning every 5-7 days, collecting egg blocks every day after spawning, and replacing the box cover with a plastic culture dish cover when the adult stage is reached, and covering the box body;
the boxes are replaced once a week, the dirty boxes are cleaned in time and used alternately, and the pairing is carried out after 1 month according to the multiple mating profits, so that the egg laying amount and the egg quality are improved;
the optimal breeding temperature is as follows: 20-30 ℃.
According to the invention, through a large number of experiments, the feeding device is improved, the maximum feeding amount of the feeding device is measured to be 7-8, and the yield of the harmonia axyridis imagoes in unit area is increased by stacking the feeding boxes; pupae are collected and feathered intensively, so that the feeding process is simplified; the method improves the egg obtaining amount by using a single-pair feeding mode, collects egg blocks by using paper strips, improves the egg collecting efficiency, and is easy to manufacture ladybug egg cards. The harmonia axyridis bred by the method has strong individual and strong predation capacity, and the hatching rate of offspring exceeds 90%. The feeding device has low cost, easy obtaining, easy indoor feeding, no environmental influence, high yield of imagoes and eggs and obvious economic and social benefits. The harmonia axyridis produced by the method can be widely applied to agricultural production as pests and natural enemies of mites.
Drawings
FIG. 1 is a schematic structural view of the present invention used in the larval stage rearing stage;
FIG. 2 is a schematic structural diagram of the present invention used in the adult stage of rearing.
Detailed Description
The invention is further illustrated below.
The invention provides a method for raising and collecting eggs of self-disabled insects and application thereof. The technical scheme adopted by the invention is as follows: firstly, a feeding box is manufactured, and the feeding box is manufactured as follows:
as shown in the figure 1-2, the feeding box is provided with a plastic transparent box body 2, the plastic box body is in a hollow cylindrical shape, the upper part of the plastic box body is large and the lower part of the plastic box body is small, an upper opening 7 of the plastic box body is 75mm in diameter, the lower bottom of the plastic box body is 55mm in diameter and 38mm in height, the volume is about 100ml, an opening of 20mm multiplied by 20mm is formed in the side surface of the plastic box body, a 100-mesh gauze is sealed on the opening to serve as a ventilation window 4, 1 sponge block 5 of 10mm multiplied by 10mm is arranged in the plastic transparent box body, and the sponge block can be soaked to provide. The feeding box is internally provided with a corrugated paper folding strip 3 with the length of 200mm and the height of 20mm along the inner wall of the box, wherein the paper folding strip is used for isolating to effectively avoid the mutual killing of larvae, and the paper folding strip is used for collecting eggs, enabling the harmonia axyridis to lay eggs on the paper strip and cutting off the eggs to manufacture the ladybug egg cards.
In order to cover the plastic transparent box, two kinds of covers are used respectively corresponding to the feeding period:
one is a plastic box cover 1 corresponding to the size of the opening of the plastic transparent box body, as shown in figure 1, the plastic box cover is provided with a buckling groove 6 corresponding to the circular opening of the plastic transparent box body, and the opening 7 of the plastic transparent box body is buckled by using the plastic box cover 1 in the larval stage feeding period;
the other type is a plastic culture dish cover 8, as shown in fig. 2, a plurality of small ventilation holes 9 penetrating through the box cover are arranged on the plastic culture dish cover along the inner periphery of the box cover, the diameter of each small ventilation hole is about 1.0mm to 2.2mm, and the plastic culture dish cover 8 is replaced by the plastic culture dish cover to cover the box body in the adult stage.
The method for breeding and collecting eggs of self-disabled insects comprises the steps of intensively breeding a small amount of harmonia axyridis larvae, collecting the larvae after pupation, intensively eclosion, breeding in a single pair after eclosion, and collecting eggs.
The larva breeding method comprises the following steps:
putting 7-8 eggs of the harmonia axyridis to be hatched into a breeding box, adding sufficient amount of eggs of the mediterranean pink borer as food, soaking sponge blocks, putting paper strips, and buckling a box cover to form an independent breeding space. A plurality of raising boxes can be stacked. The food is administered once every 2 days, 3 times in total, and 0.1g, 0.2g, and 0.3g are administered respectively.
The collection and eclosion method of the pupae comprises the following steps:
opening the breeding box after the harmonia axyridis pupates, and cutting off the paper slips where the pupae are located when the pupae are pupated on the paper slips; when pupae are formed on the box wall or the cover, the pupae are carefully shoveled down by a thin small iron sheet, and the pupae are collected in a large box for centralized eclosion.
The invention relates to a method for raising and collecting eggs of harmonia axyridis adults;
and judging the sex according to the genitals after the harmonia axyridis emerges for 2 days, and pairing to feed 1 female and 1 male in a single pair. Putting a pair of ladybugs into a breeding box, putting eggs of Mediterranean pink borer as food, soaking sponge blocks, putting paper strips, fastening a box cover, and feeding for 1 time every 2 days before spawning. Spawning begins every 5-7 days, egg blocks are collected every day after spawning, the box cover is changed into a culture dish with the diameter of 90mm, and the box can be conveniently opened every day. The harmonia axyridis loves a clean environment, otherwise, the egg laying amount is reduced; therefore, the box is replaced once a week, and the dirty box is cleaned in time for alternate use. According to the multiple mating profits, the eggs are disorganized and re-paired after 1 month, and the egg laying amount and the egg quality can be improved.
The optimum feeding temperature of the invention is as follows: 20-30 ℃.
The above description is only an example of the present invention, and all equivalent changes and modifications made according to the claims of the present invention should be covered by the present invention.
Claims (1)
1. The self-disabled insect breeding and egg collecting method is characterized in that a breeding box is firstly manufactured, and the breeding box is manufactured as follows:
the feeding box is provided with a plastic transparent box body, the plastic transparent box body is in a hollow circular or oval shape, the plastic transparent box body is large at the top and small at the bottom, the upper opening of the plastic transparent box body is 75mm in diameter, the lower bottom of the plastic transparent box body is 55mm in diameter and 38mm in height, the volume of the plastic transparent box body is about 100ml, an opening of 20mm multiplied by 20mm is formed in the side face of the plastic transparent box body, and a 100-mesh gauze is sealed on the opening to serve as a ventilation window; 1 sponge block of 10mm x 10mm is arranged in the plastic transparent box body, and the sponge block is used for providing water or hydromel after being soaked; the plastic transparent box body is provided with a corrugated folded paper strip with the length of 200mm and the height of 20mm along the inner wall of the box body, wherein the corrugated folded paper strip is used for isolating to effectively avoid the mutual killing of larvae, and the corrugated folded paper strip is used for collecting egg cards, enabling the harmonia axyridis to lay eggs on the paper strip and cutting down to manufacture the ladybug egg cards;
for covering the plastic transparent box body, two covers are respectively used corresponding to the larval stage and the adult stage:
one type is a plastic box cover corresponding to the size of a box opening of a plastic transparent box body, the plastic box cover is provided with a circular or oval buckle groove corresponding to the circular or oval box opening of the plastic transparent box body, and the plastic box cover is used for buckling the box opening of the plastic transparent box body in a larva breeding period;
the other type is a plastic culture dish cover, a plurality of small vent holes penetrating through the box cover are formed in the plastic culture dish cover along the inner periphery of the box cover, the diameter of each small vent hole is 1.0 mm-2.2 mm, the plastic culture dish cover is replaced by the plastic culture dish cover in the adult stage, and the box body is covered;
the self-mutilation insect breeding and egg collection method comprises the steps of intensively breeding a small amount of harmonia axyridis larvae, collecting the larvae after pupation for centralized eclosion, breeding in a single pair after eclosion, and collecting eggs; the specific method comprises the following steps:
the larva breeding method comprises the following steps: putting 7-8 eggs of the harmonia axyridis to be hatched into a breeding box, adding sufficient amount of eggs of the mediterranean pink borer as food, soaking sponge blocks, putting the soaked sponge blocks into corrugated paper folding strips with the length of 200mm and the height of 20mm, and buckling a box cover to form an independent breeding space; stacking a plurality of feeding boxes; the food is thrown once every 2 days, 3 times in total, and 0.1g, 0.2g and 0.3g are respectively thrown;
the collection and eclosion method of the pupa comprises the following steps: opening the breeding box after the harmonia axyridis pupates, and cutting off the paper slips where the pupae are located when the pupae are pupated on the paper slips; if pupa is formed on the box wall or the box cover, the pupa is carefully shoveled down by a thin small iron sheet, and the pupa is intensively collected and then is intensively feathered in a large box;
a method for raising and collecting eggs of harmonia axyridis adults; judging the sex according to the genitals after the harmonia axyridis emerges for 2 days, and pairing to feed 1 female and 1 male in a single pair; putting a pair of ladybugs into a breeding box, putting eggs of mediterranean pink borer as food, soaking sponge blocks, putting paper-folded paper strips, fastening a box cover, and feeding for 1 time every 2 days before spawning; spawning begins every 5-7 days, egg blocks are collected every day after spawning, and when the adult period is reached, the box cover is replaced by a plastic culture dish cover to cover the box body;
the box is replaced once a week, and the dirty box is cleaned in time for alternate use; according to the multiple mating profits, the eggs are disorganized and re-paired after 1 month, and the egg laying amount and the egg quality are improved;
the optimal breeding temperature is as follows: 20-30 ℃.
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| CN109221026B (en) * | 2018-11-20 | 2023-09-22 | 山东农业大学 | Device and method for efficiently collecting slow-eye mushroom mosquito eggs of Chinese chives |
| CN109258578B (en) * | 2018-11-21 | 2023-12-05 | 北京阔野田园生物技术有限公司 | Indoor industrial breeding method for ladybug larvae |
| CN109644950B (en) * | 2019-02-11 | 2021-11-26 | 广东省烟草南雄科学研究所 | Large-scale breeding method for red-color lygus lucorum |
| CN110036981A (en) * | 2019-04-19 | 2019-07-23 | 夏鹏亮 | A kind of harmonia axyridia oviposition device |
| CN111758672B (en) * | 2020-07-16 | 2022-02-01 | 中国热带农业科学院环境与植物保护研究所 | Group breeding method for feeding spodoptera frugiperda larvae with young corn shoots |
| CN114271239A (en) * | 2021-12-14 | 2022-04-05 | 广东省科学院南繁种业研究所 | Moth adult raising and spawning box |
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