CN109258578B - Indoor industrial breeding method for ladybug larvae - Google Patents
Indoor industrial breeding method for ladybug larvae Download PDFInfo
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- CN109258578B CN109258578B CN201811390956.1A CN201811390956A CN109258578B CN 109258578 B CN109258578 B CN 109258578B CN 201811390956 A CN201811390956 A CN 201811390956A CN 109258578 B CN109258578 B CN 109258578B
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- 238000009395 breeding Methods 0.000 title claims abstract description 37
- 241000255749 Coccinellidae Species 0.000 title claims abstract description 29
- 235000013601 eggs Nutrition 0.000 claims abstract description 53
- 230000012447 hatching Effects 0.000 claims abstract description 43
- 241000253994 Acyrthosiphon pisum Species 0.000 claims abstract description 25
- 241000199694 Harmonia axyridis Species 0.000 claims abstract description 25
- 238000004519 manufacturing process Methods 0.000 claims abstract description 19
- 239000000945 filler Substances 0.000 claims abstract description 17
- 235000010749 Vicia faba Nutrition 0.000 claims abstract description 16
- 240000006677 Vicia faba Species 0.000 claims abstract description 16
- 235000002098 Vicia faba var. major Nutrition 0.000 claims abstract description 16
- 230000019617 pupation Effects 0.000 claims abstract description 16
- 238000000034 method Methods 0.000 claims abstract description 13
- 241000238631 Hexapoda Species 0.000 claims abstract description 9
- 238000005057 refrigeration Methods 0.000 claims abstract description 8
- 229920003023 plastic Polymers 0.000 claims description 11
- 238000003860 storage Methods 0.000 claims description 10
- 241001480224 Heterodera Species 0.000 claims description 7
- 241001124076 Aphididae Species 0.000 claims description 6
- 241000196324 Embryophyta Species 0.000 claims description 4
- 238000005192 partition Methods 0.000 claims description 4
- 238000011161 development Methods 0.000 abstract description 11
- 230000018109 developmental process Effects 0.000 abstract description 11
- 238000011534 incubation Methods 0.000 abstract description 7
- 230000032669 eclosion Effects 0.000 abstract description 4
- 230000001488 breeding effect Effects 0.000 description 27
- 238000012360 testing method Methods 0.000 description 10
- 230000000694 effects Effects 0.000 description 4
- 235000013305 food Nutrition 0.000 description 4
- 230000004083 survival effect Effects 0.000 description 4
- 238000007726 management method Methods 0.000 description 3
- 238000009423 ventilation Methods 0.000 description 3
- 241000254191 Harpagophytum procumbens Species 0.000 description 2
- 244000046052 Phaseolus vulgaris Species 0.000 description 2
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 2
- 241000607479 Yersinia pestis Species 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 230000000384 rearing effect Effects 0.000 description 2
- 241000255789 Bombyx mori Species 0.000 description 1
- 241001454295 Tetranychidae Species 0.000 description 1
- 241001414989 Thysanoptera Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000009194 climbing Effects 0.000 description 1
- 230000002354 daily effect Effects 0.000 description 1
- 230000005059 dormancy Effects 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 238000009776 industrial production Methods 0.000 description 1
- 230000001418 larval effect Effects 0.000 description 1
- 238000013439 planning Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 238000012384 transportation and delivery Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
Abstract
The application discloses an indoor industrial breeding method for ladybug larvae. Selecting eggs stored for 1-4d from a refrigeration house, intensively placing the eggs into a hatching box for hatching, and then placing the initially hatched larvae into a feeding box for feeding; a layer of filter paper is paved at the bottom of the feeding box, and silk-shaped fillers are arranged in the box to enlarge the movable range of larvae; placing sufficient pea aphids in each feeding box regularly, and fixedly placing the root-cut broad bean seedlings until the larvae pupate; after pupation, the harmonia axyridis stops feeding the pea aphids, and after emergence, the larva is fed in an insect feeding cage for production. The method of the application ensures that the whole development calendar of the heteroladybug realizes industrial cultivation, ensures the consistency of incubation time, pupation time and eclosion time, is convenient for artificial centralized management and cultivation, reduces the self-phase killing rate of the heteroladybug larva period, promotes the spawning calendar of the heteroladybug, and obtains adults with regular development calendar and high-yield eggs, thereby saving the time space for industrial cultivation of the heteroladybug.
Description
Technical Field
The application relates to the technical field of natural enemy insect breeding, in particular to an indoor industrial breeding method for ladybug larvae.
Background
The heteroladybug belongs to coleopteran insects, is one of main natural enemy insects in the field, and adults and larvae of the heteroladybug can prey on aphids, thrips, spider mites, lepidopteran eggs and low-age larvae. Therefore, when the number of the harmonia axyridis reaches a certain amount, the number of field aphids and small pests can be well controlled, and crops can be protected to a certain extent. According to the predation characteristic of the harmonia axyridis, people already apply the harmonia axyridis to the control of field and greenhouse pests, and the aim of biological control is achieved. Now, tens of biological control companies exist nationwide, and most of the biological control companies are sold with ladybug products. Under the main agricultural development trend of biological control, green agriculture development, more high-quality biological control products and technologies are urgently needed. The industrial propagation cost of the harmonia axyridis is reduced, and the optimal spawning quantity of the harmonia axyridis is one of conditions for sustainable development of biological companies and is also one of important conditions for large-area popularization of natural enemy control technology.
Industrial propagation of the harmonia axyridis requires consistency of egg hatching and sufficient pea aphids. Indoor industrial breeding systems for the ladybug larvae have not been reported yet. However, the indoor breeding system is a precondition for subsequent industrial breeding of the harmonia axyridis. The existing biological companies sell the ladybug eggs externally, and the production objects are the adult ladybug eggs. The precondition of stable production and high yield of eggs per day is that the quality of the adult ladybug is better. However, most of the existing biological companies adopt a mode of purchasing adults at low price to produce spawning cards for sale, the production mode is easy to cause intermittent breeding insects, the dormancy time of the breeding insects is long, the survival rate is low, and the production requirements of the companies cannot be met. The newly emerged harmonia axyridis is undoubtedly high-quality adults, and the vitality of the adults is high, the spawning peak period is not passed, and the life duration of the adults is long, so that the artificial indoor feeding of a large number of harmonia axyridis larvae becomes an important technological breakthrough for industrial production of the harmonia axyridis eggs. However, the ladybug larvae have self-residue property, and concentrated breeding is easy to cause the self-phase killing of the larvae, so that single-head or small-quantity concentrated breeding is mostly adopted in breeding to ensure survival rate. However, the raising method has the advantages of high cost and workload, less raised larvae, and inapplicability to production, so that the self-invalidity of centralized raising of larvae is broken through technically, and the purposes of centralized raising of larvae and industrialized breeding are achieved.
Disclosure of Invention
Aiming at the problems, the application provides an indoor industrial breeding method for the ladybug larvae. Aiming at the hatching time of the harmonia axyridis eggs and the feeding and activity habit of the harmonia axyridis larvae, the application adopts a factory breeding method which is used for intensively hatching eggs, regularly picking up the initially hatched larvae, intensively breeding a large number of eggs in separate breeding boxes and periodically placing the bean seedlings and pea aphids in the breeding boxes until pupation, and simultaneously provides a centralized breeding device which is matched with the breeding method. The breeding method of the application can realize industrial breeding of the whole development calendar of the heteroladybug, ensure consistency of incubation time, pupation time and eclosion time, facilitate artificial centralized management and breeding, reduce the self-phase killing rate of the larva stage of the heteroladybug, promote the spawning calendar of the heteroladybug, and obtain adults with tidy development calendar and eggs with high yield, thereby saving time space for industrial breeding of the heteroladybug, reducing cost and improving factory benefit.
The technical scheme of the application is as follows: the indoor industrial breeding method for the ladybug larvae is characterized by comprising the following steps of:
1) Selecting eggs stored for 1-4d from a refrigeration house, intensively placing the eggs in a hatching box (hatching conditions are that the temperature is 25+/-1 ℃, the humidity is 70+/-10 percent, and the photoperiod is 16L: 8D) for hatching, and then placing the initially hatched larvae in the breeding box for breeding;
2) Placing at least 300 larvae in a feeding box, paving a layer of filter paper at the bottom of the feeding box to prevent the excessive humidity in the feeding box, and arranging filament-shaped fillers in the feeding box to increase the movable range of the larvae and reduce the contact between the larvae; sufficient pea aphids are put into each feeding box regularly, and 1-4 silkworm bean seedlings with roots cut off are fixedly put into each feeding box until larvae pupate;
preferably, the application puts 400+/-50 larvae in a feeding box, and two broad bean seedlings with roots cut off are put in the box.
3) Stopping feeding the pea aphids with the heteroladybug after pupation, and feeding the heteroladybug after emergence in an insect raising cage in a production workshop for production.
The conditions of the step 1) of the refrigeration house are as follows: the temperature is 7-10deg.C, and the humidity is 60+ -10%.
The specific operation of the step 1) of centralizing eggs in a hatching box is as follows: the egg grain number is placed according to the size of the hatching box, so that 50% of the bottom area of the hatching box is fully paved by eggs, and the hatching box is a transparent and covered sealing plastic box. The number of eggs hatched in the same batch is generally about 35% more than the number of adults to be obtained according to the production target.
And (3) observing the hatching condition of the eggs three times a day in the step 1), and setting the observation time interval according to the color change of the eggs. According to the experience and experiment of workers, the time from the breaking of the shell to the complete hatching is about 3 hours, and the time from the darkening of the egg color to the complete hatching is about 7 hours, so that the observation time can be set to 9 points, 14 points and 17 points every day. In the last observation, if the blackened egg mass is found, the egg mass can be singly taken into a unified hatching box, placed in a refrigeration house for storage overnight, and taken out at 8 points the next day for unified hatching.
The specific operation of paving a layer of filter paper at the bottom of the feeding box in the step 2) is as follows: the length and width of the filter paper are the same as those of the bottom of the feeding box, and the filter paper is placed at the bottom of the feeding box and fixed.
The step 2) is characterized in that a silk-like filler is arranged in the box, and the silk-like filler is specifically: the thread-like filler can be one or more of paper strips, thin branches and plastic strips. The paper strip can be folded, and the branches can be folded into proper lengths. Preferably, the paper strips are folded wavy paper strips, the wavy paper strips are placed in a staggered mode, the wavy paper strips are built layer by layer, and the staggered paper strip building height reaches half of the height of the feeding box.
The larva feeding in the step 2) is specifically as follows: after larvae are uniformly placed in a feeding box, two broad bean seedlings with pea aphids distributed in high density are cut and placed in the feeding box, and meanwhile, a proper amount of pea aphids are scanned from other broad bean seedlings into the feeding box by a writing brush, so that the pea aphids are sufficient to meet the feeding requirements; after two days, the broad bean seedlings are replaced and pea aphids are fed once every morning at regular time, and the method is the same as above; after 11 days, the larva of the ladybug with the harmonia axyridis starts to pupate, and at the moment, a small amount of pea aphids are only needed to be added into the feeding box, and the same batch of larva enters all pupation stages 2-3 days.
The application also provides a centralized feeding device matched with the feeding method, which comprises a feeding box, a box cover and a strand-like filler; the feeding box is a hollow box with an open top and made of transparent plastic, and the box cover is detachably covered at the open top of the feeding box; the strand-like fillers are alternately arranged in the feeding box and are used for separating the cavity in the feeding box into small partitions to separate the ladybug larvae into separate feeds.
The paper strips are folded wavy paper strips, the wavy paper strips are horizontally placed in the feeding box, and the wavy cross section of the wavy paper strips is perpendicular to the horizontal plane and parallel to the length direction of the wavy paper strips; the wavy paper strips are arranged in a multilayer superposition manner in the feeding box, the length directions of the wavy paper strips on the same layer are parallel to each other, and two adjacent wavy paper strips on the same layer are arranged in a staggered manner so that the wave crest of one wavy paper strip corresponds to the wave trough of the other wavy paper strip, and the length directions of the wavy paper strips on the lower layer and the wavy paper strips on the upper layer are mutually intersected.
The side box wall of the feeding box is provided with a vent hole, and the vent hole is covered with a 100-mesh gauze for circulation of air in the feeding box and the outside.
The box cover is provided with a plurality of feeding pipes for throwing aphids and plants into the feeding box during larva feeding, a pipe cavity in the feeding pipe penetrates through the box cover downwards, and a top pipe opening of the feeding pipe is sealed by the cover.
The feeding pipe is a cylindrical straight pipe. The cover is in threaded connection with the top end of the feeding pipe.
The beneficial effects of the application are as follows:
1) Reducing the automatic killing of larvae
The application specially designs a centralized breeding device for the harmonia axyridis larvae, which aims at the biological characteristics of the harmonia axyridis larvae, utilizes folded wavy paper strips to increase the activity space for the harmonia axyridis larvae, and simultaneously provides sufficient food to reduce the self-killing of the harmonia axyridis larvae.
The rearing box is internally provided with the broad bean seedlings, so that not only can the activity space of the ladybug larvae be increased and the humidity in the box be increased, but also the feeding pea aphids can be ensured to have edible host plants, and the feeding pea aphids can be ensured not to die in advance due to lack of hosts. According to the application, the broad bean seedlings are used for raising pea aphids, and the pea aphids are used for raising the heterodera aggregate larvae, so that sufficient feeding food is continuously provided for the heterodera aggregate larvae, and the self-phase killing of the heterodera aggregate larvae is reduced.
The method solves the problem of low survival rate of the larvae in the group culture of the harmonia axyridis production, and lays a foundation for better green agriculture of the harmonia axyridis.
2) Is convenient for artificial centralized management and breeding, saves the workload
The centralized breeding device is convenient for breeding a large number of ladybug larvae manually, and the larvae can be limited in the breeding box by arranging the box cover; the feeding pipe is arranged on the box cover and used for feeding food, so that a large number of larvae can be prevented from climbing in disorder due to opening of the box cover, and the workload is increased. Meanwhile, the method for breeding the ladybug larvae ensures the consistency of hatching time, pupation time and eclosion time, is convenient for artificial centralized management and breeding, and greatly saves the workload.
3) High quality adult easy to collect large amount of unified development period
The application is easy to collect a large number of adults with unified development duration, can ensure that female adults in each production have spawning peak periods, and avoids mature adults (spawning peak is over) in the production process, thereby improving the production efficiency and reducing the cost. Meanwhile, the whole development calendar of the harmonia axyridis can realize industrial cultivation, the spawning peak time of the harmonia axyridis can be determined, workshop production planning is easy, and the phenomenon of alternating production quantity high and low valley is avoided, so that the goods delivery condition of a later-stage company is influenced.
Drawings
Fig. 1 is a schematic perspective view of a centralized feeding device (a gauze is not arranged on a vent hole) for ladybug larvae, which is provided by the embodiment of the application;
FIG. 2 is an enlarged schematic view of the wavy paper strips of FIG. 1 placed in the feeding cassette;
in the figure: 1 feeding box, 2 box cover, 3 vent holes, 4 feeding pipe, 5 cover and 6 wavy paper strips.
Detailed Description
The following examples are illustrative of the application and are not intended to limit the scope of the application. Unless otherwise indicated, the technical means used in the examples are conventional means well known to those skilled in the art, and all raw materials used are commercially available.
The application provides a centralized feeding device for ladybug larvae, which comprises a feeding box 1, a box cover 2 and a strand-like filler; the feeding box 1 is a hollow box with an open top and made of transparent plastic, and the box cover 2 is detachably covered at the open top of the feeding box 1; the thread-like fillers are placed in the feeding box 1 in a staggered manner so as to separate the cavity in the feeding box into small partitions for separate feeding of the ladybug larvae.
In one embodiment of the present application, the thread-like filler is one or more of paper strips, thin dry branches and plastic strips.
In one embodiment of the application, the paper strip is a folded wavy paper strip 6, the wavy paper strip 6 is horizontally placed in the feeding box 1, and the wavy cross section of the wavy paper strip 6 is vertical to the horizontal plane and parallel to the length direction of the wavy paper strip 6; the wavy paper strips 6 are arranged in a multi-layer superposition manner in the feeding box 1, the length directions of the wavy paper strips 6 on the same layer are mutually parallel, two adjacent wavy paper strips 6 on the same layer are arranged in a staggered manner so as to enable the wave crest of one wavy paper strip 6 to correspond to the wave trough of the other wavy paper strip 6, and the length directions of the wavy paper strips 6 on the lower layer and the length directions of the wavy paper strips 6 on the upper layer are mutually crossed.
Preferably, the length direction of the wavy paper strips 6 positioned at the lower layer is perpendicular to the length direction of the wavy paper strips 6 positioned at the upper layer, one along the length direction of the feeding box 1 and the other along the width direction of the feeding box 1.
The wavy paper strips are placed in a staggered mode, the wavy paper strips are built layer by layer, and the staggered paper strip building height reaches half of the height of the feeding box.
In one embodiment of the application, the box cover 2 is in snap connection with the top opening of the feeding box 1.
In one embodiment of the application, the side case walls of the feeding case 1 are provided with ventilation holes 3, and the ventilation holes 3 are covered with 100 mesh gauze for ventilation of air in the feeding case 1 and the outside.
In one embodiment of the application, the box cover 2 is provided with a plurality of feeding pipes 4 for putting aphids and plants into the feeding box 1 during larval feeding, the pipe cavities in the feeding pipes 4 penetrate downwards through the box cover 2, and the top pipe ports of the feeding pipes 4 are covered by covers 5.
In one embodiment of the application, the feeding tube 4 is a straight tube in the shape of a cylinder.
In one embodiment of the application, the cap 5 is threadedly coupled to the top end of the feeding tube 4.
In one embodiment of the present application, the cap 2, the feeding tube 4 and the cover 5 are made of transparent plastic.
In one embodiment of the application, a layer of filter paper for absorbing water and preventing moisture is laid on the inner bottom surface of the feeding box 1.
The box cover 2 is not opened in the whole larva development process, more heteroladybug larvae are raised in the raising box 1, the larva can climb in disorder when the box cover 2 is opened, the workload is increased, and the larva can climb on the box cover 2, so that the buckling cover is not easy.
In the feeding process of larvae, firstly, the cover 5 is opened, few larvae in the cover 5 and the feeding pipe 4 are cleaned, and aphids and broad bean seedlings are put into the feeding box 1 from the feeding pipe 4, so that the purpose of feeding without opening the box cover 2 is achieved.
The silk-like filler is mainly used for increasing the activity space of the larvae, and can be in a silk shape or a strip shape, the length and the width of the silk-like filler can be set according to specific conditions, and the silk-like filler can be properly placed in the feeding box 1, so that the application is not limited. The shape and the size of the vent hole 3 are not specifically defined, and the vent hole 3 is automatically determined according to the size of the feeding box 1 and only needs to achieve smooth circulation of air in the feeding box 1 and the outside. The paper strips are placed in the feeding box 1 in a staggered and disordered manner so as to separate the cavity in the feeding box 1 into small partitions, the ladybug larvae are separately fed in a separated mode, the length and the width of the paper strips are not specified, the length and the width of the paper strips do not exceed the length and the width of the feeding box 1, the number of the paper strips is not specified, and a certain number of paper strips are placed according to the size of the feeding box 1.
Preferably, the feeding cassette per 400 heads/cassette is specifically: a transparent plastic box with the length of 30cm multiplied by 22cm multiplied by 13cm (length multiplied by width multiplied by height), 6 rectangular vent holes with the length of 10cm multiplied by 7cm (length multiplied by width) covered with a 100-mesh gauze are arranged on four sides of the plastic box, and a feeding pipe with the diameter of 4cm is arranged on the box cover; the feeding box is internally provided with 54 wavy paper strips of 29cm multiplied by 2cm (length multiplied by width) in a staggered way.
Example 1: hatching test of ladybug eggs
1. Eggs were selected from the 8℃freezer for storage days of 1d, 4d and 7d for testing. The number of days in the freezer is 3 for each treatment group. And egg hatching experiments were performed at 25℃and 20℃respectively.
2. And counting hatchability of 18 groups of tests, comparing hatchability differences at different temperatures of the same storage days, and comparing hatchability differences at different storage days of the same temperature.
TABLE 1 hatchability of different storage period of Hoybug eggs
Note that: 1d, 4d, and 7d in the treatment group represent the number of days stored in the refrigerator, respectively.
Through comparison, the hatching rate of the harmonia axyridis is higher and stable at 25 ℃; under the hatching condition of 25 ℃, the egg hatching rate of 1d of storage is higher. The test was carried out with a humidity of 70.+ -. 10% and a photoperiod of 16 L:8D.
Example 2 test of hatching time of Harpagophytum procumbens eggs
As can be seen from example 1, the optimal incubation temperature for the eggs of the Harpagophytum procumbens is 25 ℃. Eggs of 1d (group A), 4d (group B) and 7d (group C) were stored in a freezer for the same test, and incubation times were counted. Each treatment was repeated three times. Statistics were performed three times daily at 9, 15, and 19 points. The three statistical sums are recorded. For example, the first trial started on 14 days of 2018, 9, 15 days of 2018 as the first day, recorded as 1d, day of incubation of eggs, and so on.
TABLE 2 incubation number and incubation time of eggs with different storage times
From the above table it is clear that eggs are concentrated on day 3 hatching, i.e. hatching days 2 days. Namely, eggs stored for 1-7 days in a refrigeration house have no obvious difference in hatching uniformity. However, according to Table 1, eggs are preferably stored for 1-4 days.
Example 4
1) Eggs stored for 1d-4d are selected from the cold storage at 8 ℃ and placed in a centralized hatching box (the feeding condition is that the temperature is 25+/-1 ℃, the humidity is 70+/-10%, and the photoperiod is 16L: 8D). The number of eggs is set according to the size of the hatching box, so that 50% of the bottom area of the hatching box is fully paved by eggs, and the hatching box is a transparent plastic box with a cover and capable of being closed. The number of eggs hatched in the same batch is generally 35% more than the number of adults to be obtained according to the production objective.
The observation time can be set to 9, 14 or 17 points by observing the hatching condition of the eggs three times a day. In the last observation, if the blackened egg mass is found, the egg mass can be singly taken into a unified hatching box, placed in a refrigeration house for storage overnight, and taken out at 8 points the next day for unified hatching.
In most cases, the hatching time of the piles is consistent, so that the hatched eggs can be conveniently put into the feeding box manually.
2) Every 400+/-50 larvae are placed in a centralized feeding device shown in figure 1, and a layer of filter paper is paved at the bottom of the feeding box to prevent the excessive humidity in the feeding box. Two broad bean seedlings with the roots cut off and distributed with pea aphids at high density are cut and placed into a feeding box, and meanwhile, a proper amount of pea aphids are scanned from other broad bean seedlings into the feeding box by a writing brush. After two days, pea aphids are fed once a day in the morning, and the broad bean seedlings are replaced (the cover on the feeding pipe is opened, the pea aphids and the broad bean seedlings are put in, and the cover is screwed down) in the same way.
3) After 11 days, the larva of the ladybug with the harmonia axyridis starts to pupate, and at the moment, a small amount of pea aphids are only needed to be added into the feeding box, and the same batch of larva enters all pupation stages 2-3 days. The heterodera in pupation stage is not fed with any food. After eclosion, the ladybug with the same batch is placed in the same insect cage in a workshop and intensively fed until spawning for subsequent production.
Example 5: test of centralized breeding pupation rate of ladybug larvae
The test was performed with larvae from egg hatcheries stored in a freezer for 1 day, and three groups were repeated. Each feeding box 400-head hatched larvae were tested according to the operation method of example 4, the pupation number was counted, and the pupation rate was calculated. The test was carried out at a temperature of 25.+ -. 1 ℃ and a humidity of 70.+ -. 10% with a photoperiod of 16L: 8D. The test results are shown in Table 3.
TABLE 3 pupation Rate of larvae in rearing box
As can be seen from table 3: the method for indoor industrial feeding of larvae has average pupation rate of 76.67% and high survival rate of larvae.
Claims (3)
1. The indoor industrial breeding method for the ladybug larvae is characterized by comprising the following steps of:
1) Selecting eggs stored for 1-4d from a refrigeration house, intensively placing the eggs into a hatching box for hatching, and then placing the initially hatched larvae into a feeding box for feeding; in the hatching process, the hatching condition of eggs is observed three times a day, and the observation time is set to 9 points, 14 points and 17 points; when the eggs are observed for the last time, if the eggs with blackened eggs are found, the eggs are singly taken into a unified hatching box, placed in a refrigeration house for storage overnight, and taken out at 8 points the next day for unified hatching;
2) 400 larvae are placed in a feeding box, a layer of filter paper is paved at the bottom of the feeding box to prevent the excessive humidity in the feeding box, and strip-shaped fillers are arranged in the feeding box to enlarge the movable range of the larvae and reduce the contact between the larvae; sufficient pea aphids are put into each feeding box regularly, and two broad bean seedlings which are cut off at the root are fixedly put into each feeding box until larvae pupate;
3) Stopping feeding the pea aphids with the heterodera axyridis after pupation, and feeding the heterodera axyridis after emergence in an insect raising cage in a production workshop for production;
a centralized feeding device is adopted, which comprises a feeding box, a box cover and a strand-like filler; the feeding box is a hollow box with an open top and made of transparent plastic, and the box cover is detachably covered at the open top of the feeding box; the strand-like fillers are alternately arranged in the feeding box and are used for separating the cavity in the feeding box into small partitions to separate the ladybug larvae into separate feeds;
the strip-shaped filler is a folded wavy paper strip, the wavy paper strip is horizontally placed in the feeding box, and the wavy cross section of the wavy paper strip is perpendicular to the horizontal plane and parallel to the length direction of the wavy paper strip; the wavy paper strips are arranged in a multi-layer superposition manner in the feeding box, the length directions of the wavy paper strips on the same layer are parallel to each other, and two adjacent wavy paper strips on the same layer are arranged in a staggered manner so as to enable the wave crest of one wavy paper strip to correspond to the wave trough of the other wavy paper strip, and the length direction of the wavy paper strip on the lower layer is intersected with the length direction of the wavy paper strip on the upper layer;
the side box wall of the feeding box is provided with a vent hole, and the vent hole is covered with a 100-mesh gauze for the circulation of air in the feeding box and the outside; the box cover is provided with a plurality of cylindrical feeding pipes for throwing aphids and plants into the feeding box during larva feeding, a pipe cavity in the feeding pipe penetrates through the box cover downwards, and a top pipe opening of the feeding pipe is sealed by the cover;
the feeding boxes of every 400 heads/box are specifically: a transparent plastic box with length, width and height of 30cm, 22cm and 13cm is provided with 6 rectangular vent holes with length, width of 10cm, 7cm and 100 meshes covered on four sides, and a feeding pipe with diameter of 4cm is arranged on the box cover; 54 wavy paper strips with the length multiplied by the width of 29cm multiplied by 2cm are alternately arranged in the feeding box.
2. The method for indoor industrial feeding of the ladybug larvae according to claim 1, wherein the hatching conditions of the hatching box in the step 1) are as follows: the temperature is 25+/-1 ℃, the humidity is 70+/-10%, and the photoperiod is 16L/8D.
3. The method for indoor industrial feeding of the heterodera axyridis larvae according to claim 1, wherein the step 2) after the larvae are uniformly placed in a feeding box, broad bean seedlings cut with pea aphids are placed in the feeding box, and meanwhile, the pea aphids are scanned from other broad bean seedlings into the feeding box by a writing brush so as to meet feeding requirements; after two days, the broad bean seedlings are replaced and pea aphids are fed once every morning at regular time; after 11 days, the larva of the ladybug with the harmonia axyridis starts to pupate, and the same batch of larva enters all pupation stages after 2-3 days.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811390956.1A CN109258578B (en) | 2018-11-21 | 2018-11-21 | Indoor industrial breeding method for ladybug larvae |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811390956.1A CN109258578B (en) | 2018-11-21 | 2018-11-21 | Indoor industrial breeding method for ladybug larvae |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN109258578A CN109258578A (en) | 2019-01-25 |
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| CN109644950B (en) * | 2019-02-11 | 2021-11-26 | 广东省烟草南雄科学研究所 | Large-scale breeding method for red-color lygus lucorum |
| CN110367205B (en) * | 2019-07-19 | 2022-02-01 | 中国农业科学院植物保护研究所 | Method for feeding chrysopa perla by using pea aphid for tailbiting |
| DK3772276T3 (en) * | 2019-08-06 | 2024-01-29 | Alternative Gene Expression Sl | INSECT BREEDING BOX |
| CN110692602A (en) * | 2019-11-13 | 2020-01-17 | 北京阔野田园生物技术有限公司 | Method for large-scale production of pea aphid workshop |
| CN111374095A (en) * | 2020-02-19 | 2020-07-07 | 四川省农业科学院植物保护研究所 | Cultivation method of predatory ladybug |
| CN111758672B (en) * | 2020-07-16 | 2022-02-01 | 中国热带农业科学院环境与植物保护研究所 | Group breeding method for feeding spodoptera frugiperda larvae with young corn shoots |
| CN112042598B (en) * | 2020-07-28 | 2021-08-10 | 溧阳益植生物技术有限公司 | Whole set method for breeding harmonia axyridis |
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