CN111183960B - Armoracia chinensis ovipositing amount increasing and Armoracia chinensis egg collecting method - Google Patents
Armoracia chinensis ovipositing amount increasing and Armoracia chinensis egg collecting method Download PDFInfo
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- CN111183960B CN111183960B CN202010198084.XA CN202010198084A CN111183960B CN 111183960 B CN111183960 B CN 111183960B CN 202010198084 A CN202010198084 A CN 202010198084A CN 111183960 B CN111183960 B CN 111183960B
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- lugworm
- spawning
- egg
- eggs
- chinensis
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- 238000000034 method Methods 0.000 title claims abstract description 23
- 241000219196 Armoracia Species 0.000 title description 4
- 235000013601 eggs Nutrition 0.000 claims abstract description 57
- 241000243812 Arenicola marina Species 0.000 claims abstract description 33
- 230000001632 homeopathic effect Effects 0.000 claims abstract description 4
- 230000017448 oviposition Effects 0.000 claims description 11
- 241000156978 Erebia Species 0.000 claims description 6
- 206010021033 Hypomenorrhoea Diseases 0.000 claims description 4
- 238000005286 illumination Methods 0.000 claims description 3
- 238000011534 incubation Methods 0.000 claims description 3
- 230000000384 rearing effect Effects 0.000 claims 1
- 230000012447 hatching Effects 0.000 abstract description 8
- 241001123248 Arma Species 0.000 abstract description 7
- 230000006399 behavior Effects 0.000 abstract description 3
- 241000283618 Arma chinensis Species 0.000 description 11
- 241000607479 Yersinia pestis Species 0.000 description 8
- 241000238631 Hexapoda Species 0.000 description 5
- 238000004519 manufacturing process Methods 0.000 description 4
- 230000018109 developmental process Effects 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 2
- 241000098297 Euschistus Species 0.000 description 2
- 238000009395 breeding Methods 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- 230000000052 comparative effect Effects 0.000 description 2
- 210000004681 ovum Anatomy 0.000 description 2
- 230000002265 prevention Effects 0.000 description 2
- 241000254173 Coleoptera Species 0.000 description 1
- 102000002322 Egg Proteins Human genes 0.000 description 1
- 108010000912 Egg Proteins Proteins 0.000 description 1
- 241000258937 Hemiptera Species 0.000 description 1
- 241000255777 Lepidoptera Species 0.000 description 1
- 241000382353 Pupa Species 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000003031 feeding effect Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- 230000002045 lasting effect Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- 238000004904 shortening Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The invention discloses a method for improving the spawning amount of arma bug and collecting arma bug eggs, and belongs to the technical field of biological control. According to the lugworm spawning amount increasing and lugworm egg collecting method disclosed by the invention, lugworm spawning amount can be remarkably increased by adjusting the photoperiod, and the spawning period can also be shortened; the difficulty of egg collection can be greatly reduced through homeopathic egg collection behaviors, a large amount of eggs can be collected quickly, efficiently and conveniently, egg masses cannot be scattered, damage cannot be caused easily, and when the feeding conditions are suitable, the egg hatching rate can be guaranteed to be over 95%.
Description
Technical Field
The invention relates to the technical field of biological control, and particularly relates to a method for improving Argus armatus spawning amount and collecting Argus armatus ova.
Background
Arma chinensis belongs to the hemiptera family of the euidae, is one of important natural enemy insects which have wide predation range and strong adaptability and can be used for biological control, predates adults, pupas, larvae and eggs of various agricultural and forestry pests such as lepidoptera, coleoptera and the like, and prefers to predate pests of the spiny moth family and the golden flower family. Has obvious control effect on the increase of the number of pest populations. The arma chinensis is applied to control agricultural and forestry pests, and stable colonized populations can be formed in a release area and gradually spread to the periphery of the release area by continuously and artificially releasing for one year at a proper period as long as a place with a proper ecological environment is selected, so that the aim of continuously controlling various pests is fulfilled. The application of the arma chinensis to prevention and control of agricultural and forestry pests has an obvious and lasting effect, and the application amount of pesticides can be greatly reduced, so that the agricultural and forestry ecological environment is protected, the national green development concept is met, and the green prevention and control development direction of plant diseases and insect pests is realized.
In recent years, along with the deep development of biological control work, the protection and utilization of the arma chinensis is emphasized, and the popularization and application of the arma chinensis for controlling agricultural and forestry pests are attempted. The biological characteristics, the occurrence rule, the predation function and the like of the lugworm are reported, but the technology about the improvement of the spawning amount of the lugworm and the efficient collection of lugworm eggs is only reported at present.
At present, the cost of production of the arma chinensis is high, and the popularization and the utilization of the arma chinensis are greatly limited. In order to achieve the purpose of reducing the cost of lugworm production, each step in the existing lugworm production process needs to be technically improved, so that the operation is simpler and more convenient, the labor and the time are saved, and the feeding effect is not reduced.
Therefore, providing a method for increasing the amount of lugworm eggs laid on lugworm and collecting lugworm eggs is a problem that needs to be solved by those skilled in the art.
Disclosure of Invention
In view of the above, the invention provides a method for increasing lugworm spawning amount and collecting lugworm eggs, so as to shorten lugworm concentrated spawning time and reduce production cost.
In order to achieve the purpose, the invention adopts the following technical scheme:
a method for improving lugworm spawning amount and lugworm egg collection comprises the following specific steps:
(1) keeping the lugworm according to the normal keeping environment until adult lugworm is kept;
(2) placing the spawning container filled with the paired adults in an artificial climate box;
(3) setting the light period of the artificial climate box into two cycles, wherein the time ratio of the two cycles is 18: 6; wherein the illumination is set to 66% in 18 hours and 33% in 6 hours;
(4) collecting ovum grains by homeopathy.
Further, the normal breeding environment in the step (1) is as follows: putting the small plastic cup filled with the lugworm eggs into an artificial climate box for incubation; the temperature of the artificial climate box is controlled to be 26 +/-1 ℃, the relative humidity is 66%, and the light cycle is 16L: 8D; generally putting the euschistus chinensis into a climate box for about 7 days, and hatching 1-year nymphs from the euschistus chinensis eggs under the condition until the nymphs are raised to adults.
Further, the spawning container in the step (2) is a spawning cup.
Further, the temperature of the artificial climate box in the step (2) is 26 ℃, and the relative humidity is 75%.
According to the technical scheme, compared with the prior art, the invention discloses the method for improving the spawning amount of the arma bug and collecting the arma bug eggs, the spawning amount of the arma bug can be obviously increased by adjusting the photoperiod, and the spawning period can also be shortened; the difficulty of egg collection can be greatly reduced through homeopathic egg collection behaviors, a large amount of eggs can be collected quickly, efficiently and conveniently, egg masses cannot be scattered, damage cannot be caused easily, and when the feeding conditions are suitable, the egg hatching rate can be guaranteed to be over 95%. The invention can also select the best female insects with strong egg laying capacity by adjusting the photoperiod, thereby achieving the purpose of seed selection.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
A method for improving lugworm spawning amount and lugworm egg collection comprises the following specific steps:
(1) keeping the lugworm according to a normal breeding environment, and putting the small plastic cup containing lugworm eggs into an artificial climate box for hatching; the temperature of the artificial climate box is controlled to be 26 +/-1 ℃, the relative humidity is 66%, and the light cycle is 16L: 8D; under the condition until the adult insects are bred;
(2) placing the egg laying cup filled with the paired adults in a climatic chamber with the temperature of 26 ℃ and the relative humidity of 75 percent;
(3) setting the light period of the artificial climate box into two cycles, wherein the time ratio of the two cycles is 18: 6; wherein the illumination is set to 66% in 18 hours and 33% in 6 hours;
(4) the eggs are distributed on the gauze, paper, plants and food in the egg laying cup or on the cup wall of the egg laying cup; collecting the eggs by a homeopathic collection method, for example, when the adult insects lay eggs from left to right, the position touched by the brush pen should be the leftmost egg, that is, the egg laid out first is touched first; if the eggs are collected from the right side, the eggs are scattered and can not be taken off in a block shape, the eggs are likely to be damaged, the hatching rate is reduced, and the obtained eggs are one egg and one egg, so that the original shape of the eggs can not be maintained.
In actual operation, the lugworm oviposition behavior cannot be seen at any time, so the orientation of the eggs needs to be observed during collection, for example, the top of the eggs faces to the right, which means that the direction of spawning is from left to right, and the collection needs to be started by touching from the left.
Comparative example
A method for keeping arma chinensis and collecting arma chinensis eggs comprises the following specific steps:
putting the small plastic cup filled with the lugworm eggs into an artificial climate box for incubation; the temperature of the artificial climate box is controlled to be 26 +/-1 ℃, the relative humidity is 66%, and the light cycle is 16L: 8D; under the condition until the eggs are raised to lay eggs, the spawn is collected by touching the spawn with the writing brush head.
By adopting the method, the copulated arma chinensis can begin to lay eggs after being put into 6-7 days by adjusting the photoperiod, then eggs are laid every 1-2 days, 20-52 eggs are laid each time, the laying period can reach 35-50 days, and then the imagoes die successively; the egg laying amount can reach 616 grains at most, and is more than 400 grains on average; the hatching rate of the eggs is about 95 percent.
Adopting a comparative example method, the copulated arma chinensis bugs are generally put into 8-10 days to start spawning, then spawns are produced once every 2-3 days, 15-30 spawns are produced each time, the spawning period can reach 20-35 days, and then adults die successively; the average egg laying amount is 300 granules; the egg hatchability is 70% or even lower.
The prior patent adopts measures such as a centrifuge tube and the like to improve the egg laying amount, and does not adopt a patent or report of improving the egg laying amount in a light period regulation mode. The method has the advantages of adjusting the photoperiod, being simple and convenient to operate and strong in practicability, and being capable of improving the spawning amount and shortening the spawning period. The existing patents only mention collection of eggs, but do not specifically describe collection and methods of the eggs, so that for novices, the details are often not easy to notice, the hatching rate of the eggs is easy to reduce when the arma bugs are raised, but the problems cannot be clearly found in the later period, so that the collection method of the eggs needs to be described in detail, the novices can find the direction of the problems quickly, and the most appropriate method is adopted to ensure the hatching rate of the arma bugs.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (4)
1. A method for improving lugworm spawning amount and lugworm egg collection is characterized by comprising the following specific steps:
(1) keeping the lugworm according to the normal keeping environment until adult lugworm is kept;
(2) placing the spawning container filled with the paired adults in an artificial climate box;
(3) setting the light period of the artificial climate box into two cycles, wherein the time ratio of the two cycles is 18: 6; wherein the illumination is set to 66% in 18 hours and 33% in 6 hours;
(4) collecting the eggs along the egg laying direction by a homeopathic collection method.
2. The method for increasing Argus chinensis egg laying amount and collecting Argus chinensis eggs in claim 1, wherein the normal rearing environment in step (1) is as follows: putting the small plastic cup filled with the lugworm eggs into an artificial climate box for incubation; the temperature of the artificial climate box is controlled to be 26 +/-1 ℃, the relative humidity is 66%, and the light cycle is 16L: 8D; this condition was used until adult.
3. The lugworm spawning amount increasing and lugworm egg collecting method according to claim 1, wherein the lugworm spawning container in step (2) is a spawning cup.
4. The method for increasing Argus chinensis spawning amount and Argus chinensis egg collection according to claim 1, wherein the temperature of the artificial climate box in the step (2) is 26 ℃ and the relative humidity is 75%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010198084.XA CN111183960B (en) | 2020-03-19 | 2020-03-19 | Armoracia chinensis ovipositing amount increasing and Armoracia chinensis egg collecting method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN202010198084.XA CN111183960B (en) | 2020-03-19 | 2020-03-19 | Armoracia chinensis ovipositing amount increasing and Armoracia chinensis egg collecting method |
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| Publication Number | Publication Date |
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| CN111183960A CN111183960A (en) | 2020-05-22 |
| CN111183960B true CN111183960B (en) | 2022-03-08 |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114431194B (en) * | 2022-01-20 | 2023-03-24 | 中国农业科学院茶叶研究所 | Artificial breeding method of orius viridis |
| CN115299409A (en) * | 2022-09-01 | 2022-11-08 | 吉林省林业科学研究院 | Method for regulating large-scale breeding period of lugworm |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| BG61422B1 (en) * | 1993-08-19 | 1997-08-29 | Христо Драганов | Collector for collecting butterfly eggs |
| CN101933494A (en) * | 2010-08-27 | 2011-01-05 | 广东省昆虫研究所 | Moth-egg collecting machine |
| US10028491B2 (en) * | 2016-09-22 | 2018-07-24 | Verily Life Sciences Llc | Insect egg conveyor |
| CN207235860U (en) * | 2017-09-13 | 2018-04-17 | 天津农学院 | A kind of worm's ovum harvester suitable for insect large-scale cultivation |
| CN207531714U (en) * | 2017-11-08 | 2018-06-26 | 广州无两生物科技有限公司 | A kind of egg collector for stratiomyiid and its oviposition plate collection |
| CN209152045U (en) * | 2018-12-04 | 2019-07-26 | 贵州省烟草公司遵义市公司绥阳县分公司 | A kind of Candle-sticks stinkbug breeding apparatus convenient for collecting pieces of an egg |
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