CN115299409A - Method for regulating large-scale breeding period of lugworm - Google Patents
Method for regulating large-scale breeding period of lugworm Download PDFInfo
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- CN115299409A CN115299409A CN202211067652.8A CN202211067652A CN115299409A CN 115299409 A CN115299409 A CN 115299409A CN 202211067652 A CN202211067652 A CN 202211067652A CN 115299409 A CN115299409 A CN 115299409A
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- 238000000034 method Methods 0.000 title claims abstract description 28
- 230000001105 regulatory effect Effects 0.000 title claims abstract description 21
- 238000009395 breeding Methods 0.000 title claims abstract description 19
- 230000001488 breeding effect Effects 0.000 title claims abstract description 19
- 241000243812 Arenicola marina Species 0.000 title claims description 33
- 235000013601 eggs Nutrition 0.000 claims abstract description 50
- 230000001276 controlling effect Effects 0.000 claims abstract description 23
- 238000005286 illumination Methods 0.000 claims abstract description 20
- 241000283618 Arma chinensis Species 0.000 claims description 12
- 230000033228 biological regulation Effects 0.000 claims description 8
- 238000011161 development Methods 0.000 abstract description 27
- 241001123248 Arma Species 0.000 abstract description 21
- 241000607479 Yersinia pestis Species 0.000 abstract description 10
- 230000009025 developmental regulation Effects 0.000 abstract description 4
- 230000018109 developmental process Effects 0.000 description 25
- 241000258937 Hemiptera Species 0.000 description 14
- 230000012447 hatching Effects 0.000 description 14
- 230000000694 effects Effects 0.000 description 8
- 238000012360 testing method Methods 0.000 description 8
- 210000001161 mammalian embryo Anatomy 0.000 description 5
- 230000003203 everyday effect Effects 0.000 description 4
- 230000002265 prevention Effects 0.000 description 4
- 241000219196 Armoracia Species 0.000 description 3
- 238000011534 incubation Methods 0.000 description 3
- 239000002245 particle Substances 0.000 description 3
- 241000238631 Hexapoda Species 0.000 description 2
- 241000819999 Nymphes Species 0.000 description 2
- 210000001172 blastoderm Anatomy 0.000 description 2
- 230000002354 daily effect Effects 0.000 description 2
- 239000008187 granular material Substances 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 210000004789 organ system Anatomy 0.000 description 2
- 230000002035 prolonged effect Effects 0.000 description 2
- 241001124076 Aphididae Species 0.000 description 1
- 241000488907 Phyllocladus Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000009193 crawling Effects 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000008144 egg development Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 210000001654 germ layer Anatomy 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 230000013011 mating Effects 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000011218 segmentation Effects 0.000 description 1
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention relates to a method for regulating and controlling large-scale breeding period of arma bugs, which realizes the control of arma bugs egg period through the combination of different temperatures and illumination intensities, regulates and controls the development period of arma bugs eggs, can realize the unification of development progress, carries out development regulation and control according to pest occurrence periods, prolongs arma bugs shelf life, and solves the problem that arma bugs are unmatched in age period and control period.
Description
Technical Field
The invention belongs to the technical field of breeding, and particularly relates to a method for regulating and controlling a large-scale breeding period of lugworm.
Background
Ararma armatus Arma chinensis (Fallou) belongs to Hemiptera, ararma Arma of the Pintatomoideae of the Euschisoideae of the Euschisacae Arma, wherein Ararma Arma can prey on larvae and pupae of more than forty kinds of common and frequent pests in agriculture and forestry, such as phyllocladus armata, piercing moth, scale insect, aphid and the like, is distributed in tens of provinces in the north south of China, has strong adaptability and is suitable for artificial feeding, and under the appropriate ecological condition, the artificially released Ararma Arma can be released to breed in a release region to form a natural population and achieve the aim of controlling the pest population for a long time.
At present, arma chinensis can be bred in an artificial scale, but two main problems still exist in breeding and application, namely: 1. the nymphs of the arma chinensis at 3 ages enter a bing eating period, the wild release is ideal, but due to the biological characteristics of the arma chinensis, the spawning period of adults can last 35 days averagely, the peak period is 3-15 days, and the incubation of eggs only needs 5-7 days, so that the life history is irregular in the artificial breeding process, if the arma chinensis is released in the field in batches, the base number of the arma chinensis to be raised is large, a large amount of manpower and material resources are consumed, and the ideal prevention and treatment effect cannot be achieved; 2. due to the difference of predation capacity of the arma chinensis to pests in different ages, the release period of manually feeding the arma chinensis is not matched with the pest occurrence period, and if the arma chinensis cannot be released in a proper pest control period, the arma chinensis cannot be applied. Therefore, during the application process, the arma bug is required to be aged consistently and released in a way of matching with the effective pest control period, so that a good control effect can be achieved.
Aiming at the problems in the application process of the arma bug, how to prolong the shelf life and save the cost of manpower and material resources is particularly important.
Disclosure of Invention
In view of the problems in the prior art, the invention provides a method for regulating and controlling the large-scale breeding period of arma bug, the method provided by the invention can regulate and control the development period of arma bug eggs, ensure the uniformity of development progress, and can carry out development regulation and control according to pest occurrence periods, prolong the shelf life of arma bug, and solve the problem that the age of arma bug is not matched with the control period.
The technical scheme for solving the technical problems is as follows:
the invention provides a method for regulating and controlling a large-scale breeding period of lugworm bugs, which regulates and controls the development period of lugworm bugs eggs by regulating and controlling temperature and illumination intensity.
According to the method, the lugworm egg period is controlled through temperature and illumination intensity, the hatching time is controlled, the unification of development progress is guaranteed through regulation of the development period of lugworm eggs, development regulation is performed according to pest occurrence periods, the lugworm shelf life is prolonged, and the problem that the lugworm age period is not matched with the control period is solved.
Further, the temperature was 18 ℃.
Further, the light intensity was 90LUX.
Further, the method also comprises the step of controlling the humidity, wherein the humidity is 45%.
Further, the method also comprises the step of controlling the light cycle, wherein the light cycle is L/D =16h/8h.
Furthermore, the method also comprises the step of controlling the regulation time, wherein the regulation time is not more than 20 days.
The inventor unexpectedly discovers in the research that the egg granules with consistent development duration can be obtained while ensuring better hatching rate by adopting the conditions. In the large-scale breeding process of the lugworm bugs, a large amount of eggs can be rapidly accumulated through the regulation and control mode, the feed cost and the labor cost for regulating and controlling the lugworm bugs in nymph stages are saved, the development and development stages of the lugworm bugs can be consistent, the application of the lugworm bugs is facilitated, and the prevention and control effect is improved.
Drawings
FIG. 1 is a morphogram of Armoracia chinensis egg particle development through four stages.
Detailed Description
The principles and features of this invention are described below in conjunction with the following drawings, which are set forth by way of illustration only and are not intended to limit the scope of the invention.
Aiming at the problem of scale artificial breeding and application of arma bug, the invention controls the egg period through temperature and illumination intensity, ensures the uniformity of development progress through regulating and controlling the development period of eggs, carries out development regulation and control according to the pest occurrence period, and prolongs the shelf life of arma bug, thereby matching the age period of arma bug with the prevention and treatment period.
The experimental result of the invention is unexpected to discover that after arma chinensis eggs are regulated and controlled for 20 days under the conditions of temperature of 18 ℃, humidity of 45%, photoperiod L/D =16h/8h and illumination intensity of 90LUX, the hatchability can reach more than 70%, and the large-scale breeding and the regular development progress are realized while a large amount of manpower and food cost is saved, thereby being beneficial to release and application.
In various embodiments, the method for culturing test insects comprises the steps of: keeping the armarium chinensis to adults under the conditions of the laboratory temperature of 28 ℃, the humidity of 45 percent and natural illumination, wherein the photoperiod L/D =16h/8h, selecting one of each adult female and male to arrange in an insect box for mating and spawning, selecting spawn grains in the spawning peak period of 3-10 days, manually collecting the spawn every day, and placing the collected spawn grains in a plastic culture dish paved with filter paper for testing in a culture box.
Example 1
As shown in FIG. 1, armoracia chinensis egg particle development proceeds through four stages, namely, the first stage (A), the second stage (B), the third stage (C) and the fourth stage (D).
The first stage is as follows: the egg grains are milky white or light gray, and the surface is villous.
And a second stage: the whole egg particle is gray brown, and the surface of the egg is seen to generate black spots under a stereoscope, and at the moment, the blastoderm, the embryo ribbon, the germ layer and the embryo membrane of the egg are gradually formed.
And a third stage: the egg granules are brownish yellow, and the embryo body segmental tissue can be seen on the surface of the egg, and at the moment, the embryo body segmental and appendage forming process is carried out, and an organ system is gradually formed.
A fourth stage: the eggs are black brown, organ systems in the eggs grow mature, the back combination is completed, and the nymphs which are bred to be hatched initially can be hatched quickly.
The experimental method comprises the following steps:
test groups: the lugworm eggs collected every day are placed in a plastic culture dish paved with filter paper and put into incubators with different culture conditions. The test groups were set up as 9 groups, 1 st to 9 th. The humidity of each test group was 45%, the photoperiod L/D =16h/8h, and the temperature and illumination intensity are shown in table 1.
TABLE 1 different temperature and light intensity combinations
Control group: putting the lugworm eggs collected daily in a plastic culture dish paved with filter paper, wherein the incubation condition is that the humidity is 45%, the photoperiod L/D =16h/8h, the temperature is 28 ℃, natural illumination is carried out, and the development progress is observed under a daily stereoscope.
The experimental results are shown in table 2, the overall development progress of the nymphs which can be hatched by processing the eggs under different conditions is approximately the same, the development progress of the second stage and the third stage of the eggs can be obviously slowed down under the conditions of 15 ℃ and 18 ℃, so that the hatching time is prolonged, compared with a control group, the development time is about 4 days from the second stage to the fourth stage under the condition of 15 ℃ illumination intensity of 30LUX, the development time is about 20 days from the third stage, and the nymphs which are hatched about 1-2 days after being taken back to the room temperature; in contrast, 90LUX was significantly accelerated from egg development to the second and third stages, to the third stage at around 12 days and to the fourth stage at around 20 days.
Under the condition of 18 ℃, the egg grains enter the second stage after about 2 days of development, when the egg grains are treated to about 12 days, most of the egg grains can develop to the third stage, about 18 days can develop to the fourth stage, a few egg grains can hatch out the primarily hatched nymphs, but the primarily hatched nymphs are not active, and then the feeding is not needed. And after 20 days, placing the eggs at room temperature, hatching the eggs for about 1-2 days, basically crawling the hatched nymphs which are hatched initially, and transferring the eggs to a preservation box to feed the nymphs.
The above studies indicate that low temperature has significant slowing down effect on the second and third stages of development of lugworm eggs during the process of treating lugworm eggs, indicating that the eggs are more sensitive to temperature during blastoderm development and embryo segmentation.
TABLE 2 Effect of different conditions on the development history
Note: the developmental cycle refers to the development stage in the first day, such as the 30LUX condition at 15 deg.C, the first stage at 3 + -1 d, the second stage at 4 + -2d, the third stage at 20 + -4 d, and the fourth stage at 21 + -1 d.
Example 2 Effect of different temperatures and illumination intensities on Armoracia chinensis egg hatchability
The lugworm eggs collected every day are placed in a plastic culture dish paved with filter paper and put in incubators with different culture conditions. The test groups were set up in 9 groups, 1 st to 9 th. The humidity of each test group was 45%, the photoperiod L/D =16h/8h, and the temperature and light intensity are shown in table 3.
TABLE 3 different temperature and light intensity combinations
A control group (CK) is additionally provided, and the hatching conditions of the control group are as follows: humidity is 45%, photoperiod L/D =16h/8h, temperature is 28 ℃, and natural illumination is carried out.
Both the test and control groups were 200 eggs/group, with 5 replicates per combination. Observing and recording the hatching condition day by day, after 20 days, placing the unhatched eggs in the control group condition for further hatching (if hatching can be realized, hatching can be realized within three days generally), and counting the hatching rate.
The experimental results are shown in table 4, no matter the illumination intensity, the development of the lugworm ova can not be inhibited under the condition of 25 ℃, compared with the control group, the lugworm ova can be hatched for 5-7 days under the condition of 25 ℃, wherein the hatching rate is the highest (86%) under the condition of 90LUX illumination intensity. The temperature is 15 ℃ and 18 ℃, the development of the lugworm ova can be obviously slowed down, the lugworm ova can be taken back to the room temperature after being treated for 20 days, the lugworm ova can be incubated within 3 days, however, the hatching rate of the lugworm ova treated at the same temperature under different illumination intensities is obviously different, the hatching rate of the lugworm ova treated at 18 ℃ is obviously higher than that of the LUX treated at 15 ℃, and the hatching rate can reach 75.67% under the condition of 18 ℃ under 90LUX illumination intensity. The hatchability of 90LUX light intensity at 15 ℃ was reduced by 44% compared to the control, whereas the hatchability of 90LUX light intensity at 18 ℃ was reduced by only 11.33% compared to the control.
TABLE 4 Effect of different conditions on hatchability
When the method is specifically applied, the arma bug eggs laid firstly can be controlled under the conditions that the humidity is 45%, the photoperiod L/D =16h/8h, the temperature is 18 ℃ and the illumination intensity is 90LUX, the regulation and control time is preferably not more than 20 days, and the arma bug eggs laid in the spawning period are uniformly hatched continuously after the arma bug eggs are collected.
For example: taking 20 days as an example, regulating and controlling Arcoliform ova produced every day under the conditions of humidity of 45%, photoperiod L/D =16h/8h, temperature of 18 ℃ and illumination intensity of 90LUX. After collecting Argy bugs eggs on the 20 th day, the Argy bugs eggs are incubated with the regulated Argy bugs eggs. The incubation conditions may be: humidity is 45%, photoperiod L/D =16h/8h, temperature is 28 ℃, and natural illumination is carried out. The method can ensure that the Argy chinensis eggs collected on the 1 st day and other days are incubated together, and further ensure that the life history of the Argy chinensis eggs is tidy.
In the large-scale breeding process of the lugworm bugs, the large amount of eggs can be rapidly accumulated by adopting the regulation and control mode, and the feed cost and the labor cost for regulating and controlling the lugworm bugs in the nymph stage are saved, so that the lugworm bugs can be kept consistent in growth process, can be hatched at the same time, are convenient to apply, and improve the prevention and control effect.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.
Claims (6)
1. A method for regulating and controlling a large-scale breeding period of lugworm is characterized in that the growth period of lugworm eggs is regulated and controlled by regulating and controlling temperature and illumination intensity.
2. The method for regulating and controlling large-scale breeding cycle of armadilla chinensis as claimed in claim 1, wherein the temperature is 18 ℃.
3. The method for regulating and controlling large-scale breeding cycle of arma chinensis as claimed in claim 1, wherein the illumination intensity is 90LUX.
4. The method for regulating and controlling the scale breeding cycle of lugworm bug according to any one of claims 1-3, further comprising controlling humidity to 45%.
5. The method for regulating and controlling the scale breeding cycle of lugworm according to any one of claims 1-3, further comprising controlling the photoperiod, wherein the photoperiod is L/D =16h/8h.
6. The method for regulating and controlling the large-scale breeding period of lugworm according to any one of claims 1-3, further comprising controlling the regulation and control time, wherein the regulation and control time is not more than 20 days.
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109757441A (en) * | 2019-03-13 | 2019-05-17 | 中国农业科学院植物保护研究所 | A method of storage Candle-sticks stinkbug |
| CN110476901A (en) * | 2019-09-17 | 2019-11-22 | 中国农业科学院植物保护研究所 | A method of extending Candle-sticks stinkbug ovum shelf life |
| CN111183960A (en) * | 2020-03-19 | 2020-05-22 | 中国烟草中南农业试验站 | Armoracia chinensis ovipositing amount increasing and Armoracia chinensis egg collecting method |
| CN111657235A (en) * | 2020-07-08 | 2020-09-15 | 中国烟草中南农业试验站 | Armoracia chinensis breeding method |
| CN114521537A (en) * | 2022-02-17 | 2022-05-24 | 中国农业科学院植物保护研究所 | Lugworm diapause induction method and long-term storage method |
-
2022
- 2022-09-01 CN CN202211067652.8A patent/CN115299409A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109757441A (en) * | 2019-03-13 | 2019-05-17 | 中国农业科学院植物保护研究所 | A method of storage Candle-sticks stinkbug |
| CN110476901A (en) * | 2019-09-17 | 2019-11-22 | 中国农业科学院植物保护研究所 | A method of extending Candle-sticks stinkbug ovum shelf life |
| CN111183960A (en) * | 2020-03-19 | 2020-05-22 | 中国烟草中南农业试验站 | Armoracia chinensis ovipositing amount increasing and Armoracia chinensis egg collecting method |
| CN111657235A (en) * | 2020-07-08 | 2020-09-15 | 中国烟草中南农业试验站 | Armoracia chinensis breeding method |
| CN114521537A (en) * | 2022-02-17 | 2022-05-24 | 中国农业科学院植物保护研究所 | Lugworm diapause induction method and long-term storage method |
Non-Patent Citations (1)
| Title |
|---|
| 孙明雅等: "《马尾松毛虫天敌图志》", vol. 1, 广西人民出版社, pages: 119 - 122 * |
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