CN105145487A - Trichogramma japonicun ashmead artifical breeding production method - Google Patents
Trichogramma japonicun ashmead artifical breeding production method Download PDFInfo
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- CN105145487A CN105145487A CN201410354251.XA CN201410354251A CN105145487A CN 105145487 A CN105145487 A CN 105145487A CN 201410354251 A CN201410354251 A CN 201410354251A CN 105145487 A CN105145487 A CN 105145487A
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Abstract
The invention provides a method for breeding trichogramma japonicun ashmead by means of beet armyworm ovums. The method is relatively high in production efficiency and short in breeding period, and is beneficial to scale production of trichogramma japonicun ashmead. The method comprises following steps of 1) producing beet armyworm ova; and 2) breeding trichogramma japonicun ashmead by means of the beet armyworm ova. The trichogramma japonicun ashmea bred artificially are used for controlling agricultural pests which mainly comprise rice-stem borer, yellow rice borer and rice leaf roller. Compared with the prior art, the trichogramma japonicun ashmead artifical breeding production method is advantaged in that beet armyworm can be obtained easily, and can be bred simply; and the method is relatively high in production efficiency and short in breeding period, and is convenient for large-scale breeding, and is beneficial to scale production of trichogramma japonicun ashmead.
Description
Technical field
The invention belongs to the technical field of biological control of pest, relate to the breeding production method of oophagous trichogrammae, particularly a kind of artificial breeding production method of Trichogramma.
Background technology
Oophagous trichogrammae is most study in whole world biological control of insect pests, a most widely used class insect, has 12 kinds at present at least by large scale artificial breeding, in order to prevent and treat some important agriculture and forestry injurious insects.Breeding in a large number of oophagous trichogrammae population mainly utilizes vector ovum to carry out, and the vector ovum of oophagous trichogrammae utilizes more successfully egg of oak silkworm, Eggs of Philosamia Cynthia Ricini, gelechiid ova and rice moth egg.Large ovum (egg of oak silkworm, Eggs of Philosamia Cynthia Ricini) is owing to obtaining easily, and advantage of lower cost, so the large egg parasite utilizing egg of oak silkworm etc. to breed such as trichogramma dendrolimi energy large-scale application.The insects such as trichogramma dendrolimi control of maize snout moth's larva are utilized to be that China uses the most successful biological and ecological methods to prevent plant disease, pests, and erosion technology, at present in China northeast, the North China control gross area is more than 4,500 ten thousand mu, and production technology is ripe, family of factory's (station) more than 10, input-output ratio, up to more than 1: 20, corn borer preventing and controlling tentatively achieves the target of " public plant protection, environment protective plant protecting ".
Trichogramma is the important parasite of the rice grubs such as rice-stem borer, paddy stem borer and rice leaf roller; the host range of Trichogramma is narrow, still have any problem with striped rice borer and these primary host's mass rearings of rice leaf roller; normally utilize rice moth egg as an alternative host breed Trichogramma; but also there is the defects such as large-scale production difficulty, host breeding cycle be longer in this production model; it is existing for many years that China utilizes rice moth egg to produce Trichogramma; but be difficult to form scale, commercialization, limit the production of organic paddy rice.The Trichogramma parasitic egg stage of Ostrinia furnacalis of energy and mythimna separata ovum, but the ovum of these two kinds of insects becomes block, not easily takes off from utensil of laying eggs and makes ovum card, limit application; Diamond-back moth life cycle is short, and fertility is high, can expand population rapidly in a short time, obtains a large amount of ovum, but its ovum grain is little, and cause the Trichogramma quality of breeding to decline, equally, Eggs of Diamondback Moth also not easily makes ovum card, forms standardization; The egg laying amount of prodenia litura is large, also easily can make the ovum card of standard, but can not complete growth after Trichogramma parasitism.Therefore screening the high Hosts of a kind of production efficiency is utilize oophagous trichogrammae as an important foundation of paddy field biological control.
Summary of the invention
Based on above object, the invention provides a kind of artificial breeding production method of Trichogramma.The invention provides a kind of method utilizing Beet Armyworm Eggs to breed Trichogramma, method production efficiency provided by the invention is higher, and the breeding cycle is short, is conducive to large-scale production Trichogramma.
On the one hand, the invention provides a kind of artificial breeding production method of Trichogramma, wherein, described method comprises the step using Beet Armyworm Eggs to breed Trichogramma.
Preferably, said method comprising the steps of:
1) Beet Armyworm Eggs is produced;
2) Beet Armyworm Eggs is used to breed Trichogramma.
Preferably, described step 1) comprise the raising of beet exigua larvae, the collection of pupa, imago breeding, the collection of ovum;
Preferably, described step 1) in the condition of raising be the temperature of 24-30 DEG C, the humidity of 60%-70%; More preferably, temperature is 27 ± 1 DEG C, and humidity is 65%;
More preferably, described step 1) comprise the following steps:
A) select the Beet Armyworm Eggs being about to hatching to put into hatching house to hatch, hatching 2-3 days;
Preferably, in described larvae hatch room, feed is added;
Preferably, the temperature of described hatching house is 24-30 DEG C, and humidity is 60%-70%; More preferably, temperature is 27 ± 1 DEG C, and humidity is 65%;
B) after hatching, regrowth 4-6 days, larva is transferred to larva raising room and raises, and add feed, the larva raising phase is 10-15 days;
Preferably, described feed is the artificial feed being suitable for lepidopteran insect larvae, and in a preferred embodiment, feed used in this application is according to the materials and methods preparation provided in the patent No. Chinese patent application that is 200410039466.9;
C) pupated, behind at least one sky, by the pupa of beet armyworm, to use 4% formalin sterilization 20-40 minute, dry; Wherein beet armyworm pupa time is 5-7 days;
D) before pupa is sprouted wings, pupa is put into laying coop, wherein, Adult worms producting eggs early stage 1-3 days, egg-laying period 4-6 days.
Preferably, the temperature of described laying coop is 24-30 DEG C, and humidity is 60%-70%; More preferably, temperature is 27 ± 1 DEG C, and humidity is 65%;
E) Beet Armyworm Eggs is collected.
Preferably, Beet Armyworm Eggs described in selected part is used for conservation;
Preferably, will the Beet Armyworm Eggs 4% formalin sterilization 20-40 minute of conservation be used for, after drying, raise beet armyworm for standby.
More preferably, the Beet Armyworm Eggs being used for connecing honeybee is divided into simple grain, makes ovum card.
Preferably, every square centimeter of described ovum card has Beet Armyworm Eggs 380 ± 20 ovum;
Preferably, use 40 mesh sieves that described Beet Armyworm Eggs is divided into simple grain;
Preferably, deactivation under described ovum card use ultraviolet;
More preferably, described inactivation time is 20-40 minute;
Preferably, described step 2) be:
I) in numerous honeybee room, keep temperature 24-30 DEG C, humidity 70%-90%, breed than 1:5 ~ 15 according to the kind honeybee of Trichogramma and Beet Armyworm Eggs, take out after 1 day;
Preferably, described numerous honeybee room temperature remains on 27 ± 1 DEG C, humidity about 80%;
Preferably, described numerous honeybee room keeps dark state.
II) egg parasitoid completing the process of laying eggs is put into culturing room, be cultured to before Trichogramma grows pupa, then store.
Preferably, the temperature of described culturing room remains on 24-30 DEG C, humidity 70%-90%; More preferably, described culturing room temperature remains on 27 ± 1 DEG C, humidity about 80%;
Preferably, by described save as be placed in 5-7 DEG C refrigerating box in store.
Preferably, in described step 2) in use Beet Armyworm Eggs card to breed, then store ovum card;
Preferably, by step 2) in complete the process of laying eggs Trichogramma egg parasitoid be prepared as ovum card;
Preferably, every square centimeter of described ovum card has Trichogramma egg parasitoid 380 ± 20;
Preferably, deactivation under described ovum card use ultraviolet; More preferably, described inactivation time is 20-40 minute;
Preferably, before described ovum is stuck in field adaptability, temperature 27 ± 1 DEG C, humidity 70%-90% heats growth;
Wherein, described in time of heating pest development progress of preventing and treating according to each field, determine after the conditions such as local weather forecast consider.
On the other hand, present invention also offers a kind of method preventing pest, wherein said method uses the Trichogramma control pest of artificial breeding, and described pest are mainly rice-stem borer, paddy stem borer, rice leaf roller.
Compared with prior art, the present invention has the following advantages:
1) beet armyworm used in the inventive method is originated easily, it is easy to raise;
2) method production efficiency provided by the invention is higher, and the breeding cycle is short, is convenient to breed on a large scale, is conducive to large-scale production Trichogramma.
Accompanying drawing explanation
Below, describe embodiment of the present invention in detail by reference to the accompanying drawings, wherein:
Fig. 1 is the flow chart of a specific embodiment of the present invention;
Embodiment
Unless specifically stated otherwise, Beet Armyworm Eggs used in following examples can purchased from animal institute of the Chinese Academy of Sciences.
Unless specifically stated otherwise, composition used in following examples is the composition analyzing pure rank, and can obtain from being normally purchased channel.
embodiment 1 produces Beet Armyworm Eggs
1. the raising of beet armyworm, insectary temperature controls at 27 ± 1 DEG C, humidity about 65%.
The raising of A larva; The Beet Armyworm Eggs of soon hatching is placed on culture dish side, and adds appropriate artificial feed at opposite side, cover the lid with toilet paper, in order to avoid larva escapes.Every ware about 0.1 gram of ovum, hatches after 2 days, can be split by the larva of a ware in 2 or 3 wares and raise; After regrowth 2-3 days, be transferred in large square box by larva and raise, 2-3 ware larva can be merged in a large square box, and adds artificial feed; Can adjust each large square box larva quantity according to actual conditions, before pupating, every large square box about has about 400 larvas, about 12 days beet exigua larvae phases.Before pupating, by multilayer toilet paper place mat in large square box side, it is made to pupate smoothly.
B receives pupa; After having pupated about 1 day, tear toilet paper, shaken off the pupa of beet armyworm, the larva do not pupated has been abandoned, picks up foreign material clearly, with 4% formalin sterilization pupa 30 minutes, dried.About 7 days pupa time of beet armyworm.
C Adult worms producting eggs; Before emergence, pupa is put into cage of laying eggs, after emergence, 10% hydromel of feeding, put into and lay eggs with plastic sack, beet armyworm lays eggs on plastic sack, Adult worms producting eggs about 2 days in earlier stage, about about 5 days egg-laying period
The collection process of D ovum; Plastic sack is overturn, shakes off and shake loose the Beet Armyworm Eggs on plastic sack.Part ovum can be sterilized 30 minutes by 4% formalin, after drying, for breeding beet armyworm.
embodiment 2 breeds Trichogramma
The making of 1 ovum card, crosses 40 mesh sieves by the Beet Armyworm Eggs being used for connecing honeybee, is divided into simple grain, ovum is poured on and scribbles glue or with being stained with on the scraps of paper of double faced adhesive tape, making ovum card, drying, (every square centimeter about has Beet Armyworm Eggs 380 ovum), deactivation 30 minutes under 15 watts of ultraviolets.
2. numerous honeybee room temperature remains on 27 ± 1 DEG C, humidity about 80%, and because Trichogramma has phototaxis, generally when connecing honeybee, numerous honeybee room is dark.The ovum card made to be put into than 1:10 according to honeybee ovum and connects honeybee box, take out after 1 day.Put into kind of honeybee and new ovum card every day.Place 3 days at the ovum having connect honeybee is stuck in 27 ± 1 DEG C, before treating that Trichogramma grows pupa.Be stored in the refrigerating box of 5-7 DEG C.
embodiment 3 compared with the prior art and cost accounting
The Trichogramma parasitic egg stage of Ostrinia furnacalis of energy and mythimna separata ovum, but the ovum of these two kinds of insects becomes block, not easily takes off from utensil of laying eggs and makes ovum card, limit application; Diamond-back moth life cycle is short, and fertility is high, can expand population rapidly in a short time, obtains a large amount of ovum, but its ovum grain is little, and cause the Trichogramma quality of breeding to decline, equally, Eggs of Diamondback Moth also not easily makes ovum card, forms standardization; The egg laying amount of prodenia litura is large, also easily can make the ovum card of standard, but can not complete growth after Trichogramma parasitism.Therefore screening the high Hosts of a kind of production efficiency is utilize oophagous trichogrammae as an important foundation of paddy field biological control.
Current rice moth egg is the main Hosts of breeding Trichogramma, and the generation time of usual rice moth at 26-28 DEG C is about 60 days, breeding rate about 50 times.Beet armyworm generation time at the same temperature about 23 days, breeding rate is about 100, and so same breeding rate in 60 days reaches tens0000 times; Far away higher than the reproductive efficiency of rice moth.Through the cost accounting of large-scale breeding beet armyworm, 10,000 pupa costs are about 100 yuan, and 10,000 pupas can add numerous honeybee cost by numerous honeybee 2,000,000 (laying eggs 200 Granule Computing with average every pupa), are no more than 150 yuan.So the cost of every ten thousand honeybees is less than 1 yuan, be significantly less than the cost of rice moth.Use the method for the application, can how for breeding Trichogramma, population does not degenerate, and R0 remains on more than 10 times, thus makes merchandized handling Trichogramma, and large-area applications becomes possibility in the biological control of the snout moth's larva of rice.
Specific description of embodiments of the present invention does not above limit the present invention, and those skilled in the art can make various change or distortion according to the present invention, only otherwise depart from spirit of the present invention, all should belong to the scope of claims of the present invention.
Claims (10)
1. an artificial breeding production method for Trichogramma, wherein, described method comprises the step using Beet Armyworm Eggs to breed Trichogramma.
2. method according to claim 1, wherein, said method comprising the steps of:
1) Beet Armyworm Eggs is produced;
2) Beet Armyworm Eggs is used to breed Trichogramma.
3. method according to claim 2, wherein, described step 1) be that method by comprising the following steps realizes:
The raising of beet exigua larvae, the collection of pupa, imago breeding, the collection of ovum;
Preferably, described step 1) in the condition of raising be the temperature of 24-30 DEG C, the humidity of 60%-70%; More preferably, temperature is 27 ± 1 DEG C, and humidity is 65%.
4. method according to claim 3, wherein, described step 1) be that method by comprising the following steps realizes:
A) Beet Armyworm Eggs being about to hatching is selected to put into hatching house, hatching 2-3 days;
Preferably, in described larvae hatch room, feed is added;
Preferably, the temperature of described hatching house is 24-30 DEG C, and humidity is 60%-70%; More preferably, temperature is 27 ± 1 DEG C, and humidity is 65%;
B) after hatching, regrowth 4-6 days, larva is transferred to larva raising room and raises, and add feed, the larva raising phase is 10-15 days;
Preferably, described feed is the artificial feed being suitable for lepidopteran insect larvae;
C) pupated, behind at least one sky, by the pupa of beet armyworm, to use 4% formalin sterilization 20-40 minute, dry; Wherein beet armyworm pupa time is 5-7 days;
D) before pupa is sprouted wings, pupa is put into laying coop, wherein, Adult worms producting eggs early stage 1-3 days, egg-laying period 4-6 days;
Preferably, the temperature of described laying coop is 24-30 DEG C, and humidity is 60%-70%; More preferably, temperature is 27 ± 1 DEG C, and humidity is 65%;
E) Beet Armyworm Eggs is collected.
5. method according to claim 4, wherein, step e) Beet Armyworm Eggs collected may be used for conservation;
Preferably, will the Beet Armyworm Eggs 4% formalin sterilization 20-40 minute of conservation be used for, then dry.
6. the method according to any one of claim 1-5, wherein, step e) Beet Armyworm Eggs collected may be used for breeding Trichogramma;
The Beet Armyworm Eggs being used for breeding Trichogramma is divided into simple grain, makes ovum card;
Preferably, every square centimeter of described ovum card has Beet Armyworm Eggs 380 ± 20 ovum;
Preferably, use 40 mesh sieves that described Beet Armyworm Eggs is divided into simple grain;
Preferably, deactivation under described ovum card use ultraviolet;
More preferably, described inactivation time is 20-40 minute.
7. method according to claim 2, wherein, described step 2) be that method by comprising the following steps realizes:
I) in numerous honeybee room, keep temperature 24-30 DEG C, humidity 70%-90%, breed than 1:5 ~ 15 according to the kind honeybee of Trichogramma and Beet Armyworm Eggs, take out after 1 day;
Preferably, described numerous honeybee room temperature remains on 27 ± 1 DEG C, humidity about 80%;
Preferably, described numerous honeybee room keeps dark state;
II) ovum completing the process of laying eggs is put into culturing room, be cultured to before Trichogramma grows pupa, then store;
Preferably, the temperature of described culturing room remains on 24-30 DEG C, humidity 70%-90%; More preferably, described culturing room temperature remains on 27 ± 1 DEG C, humidity about 80%;
Preferably, the interior storage of the refrigerating box being placed in 5-7 DEG C is saved as described in.
8. method according to claim 7, wherein, in described step 2) in use Beet Armyworm Eggs card to breed, then store ovum card;
Or, by step 2) in complete the process of laying eggs Trichogramma egg parasitoid be prepared as ovum card;
Preferably, every square centimeter of described Trichogramma egg parasitoid ovum card has Trichogramma egg parasitoid 380 ± 20;
Preferably, deactivation under described Trichogramma egg parasitoid ovum card use ultraviolet; More preferably, described inactivation time is 20-40 minute.
9. the method according to any one of claim 1-8, wherein, described Trichogramma egg parasitoid before field adaptability, temperature 27 ± 1 DEG C, growth of heating under the condition of humidity 70%-90%;
Preferably, described in time of heating pest development progress of preventing and treating according to each field, determine after the conditions such as local weather forecast consider.
10. prevent a method for pest, wherein said method uses the Trichogramma control pest of artificial breeding, and described pest are rice-stem borer, paddy stem borer and/or rice leaf roller.
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| CN108056070A (en) * | 2018-01-16 | 2018-05-22 | 吉林农业大学 | Utilize the method for white snow moths attracted by lamplight breeding trichogramma |
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| CN113349166A (en) * | 2021-07-23 | 2021-09-07 | 福建省农业科学院水稻研究所 | Method for breeding rice louse trichogramma in batches |
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| CN105875519A (en) * | 2016-06-08 | 2016-08-24 | 中国热带农业科学院环境与植物保护研究所 | Method for efficiently reproducing brachymeria lasus by taking corcyra cephalonica pupae as hosts |
| CN106689063A (en) * | 2016-12-07 | 2017-05-24 | 中国科学院动物研究所 | Artificial breeding production method of trichogramma japonicum |
| CN108056070A (en) * | 2018-01-16 | 2018-05-22 | 吉林农业大学 | Utilize the method for white snow moths attracted by lamplight breeding trichogramma |
| CN108056070B (en) * | 2018-01-16 | 2021-11-12 | 吉林农业大学 | Method for breeding trichogramma by using snowflake moth |
| CN110521666A (en) * | 2018-05-25 | 2019-12-03 | 田志伟 | A kind of human assistance mating system of Black-winged Stilt |
| CN112219796A (en) * | 2018-05-30 | 2021-01-15 | 吉林省林业科学研究院 | Application of cultured Chouioia cunea Yang in preventing and treating Pinctada acutifolia Walker |
| CN112219796B (en) * | 2018-05-30 | 2022-01-14 | 吉林省林业科学研究院 | Application of cultured Chouioia cunea Yang in preventing and treating Pinctada acutifolia Walker |
| CN113349166A (en) * | 2021-07-23 | 2021-09-07 | 福建省农业科学院水稻研究所 | Method for breeding rice louse trichogramma in batches |
| CN113508713A (en) * | 2021-07-30 | 2021-10-19 | 中国农业科学院植物保护研究所 | Method for breeding trichogramma by using eggs of tuber moths of potatoes |
| CN114651793A (en) * | 2022-05-01 | 2022-06-24 | 中国医学科学院药用植物研究所云南分所 | Artificial breeding of trichogramma obscura and method for preventing and treating spodoptera frugiperda by using trichogramma obscura |
| CN114651793B (en) * | 2022-05-01 | 2023-04-18 | 中国医学科学院药用植物研究所云南分所 | Artificial breeding of trichogramma obscura and method for preventing and treating spodoptera frugiperda by using trichogramma obscura |
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