CN103444633A - Synergistic processing method for breeding trichogramma chilonis by using oak silkworm egg - Google Patents
Synergistic processing method for breeding trichogramma chilonis by using oak silkworm egg Download PDFInfo
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- CN103444633A CN103444633A CN2013103642477A CN201310364247A CN103444633A CN 103444633 A CN103444633 A CN 103444633A CN 2013103642477 A CN2013103642477 A CN 2013103642477A CN 201310364247 A CN201310364247 A CN 201310364247A CN 103444633 A CN103444633 A CN 103444633A
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Abstract
The invention discloses a synergistic processing method for breeding trichogramma chilonis by using an oak silkworm egg, and trichogramma chilonis is bred by using the oak silkworm egg under a manual controlled condition. The method comprises the following steps of soaking the surface of a fresh oak silkworm disemboweled egg by a preprocessed solution; taking out and airing the oak silkworm disemboweled egg to be used as a progeny egg for receiving the trichogramma chilonis; after the eclosion of the trichogramma chilonis from a parental egg, placing the parental egg and the progeny egg with the preprocessed surface together in a dark environment; after a parasitism link, removing the parental egg and the trichogramma chilonis; retaining the progeny egg, wherein the growth and eclosion of the trichogramma chilonis are completed under conditions that the wavelength of a blue light is 475-495 nm and the illumination intensity is 500-1000 lux. In large-scale reproduction, various development indexes of the trichogramma chilonis are better; the stability is stronger. Comparing the method with the traditional method, the parasitism rate can be increased by 4-26 percentage points; the trichogramma chilonis number of a single egg is moderate; the uniformity degree of samples in a sampling room is better; the eclosion rate can be increased by 0.2-4 percentage points; the parasitism rate is increased and maintained at a higher level; the trichogramma chilonis left rate is reduced by 1-3 percentage points; the uniformity degree of samples in the sampling room is better.
Description
Technical field
The present invention discloses the synergistic processing method that a kind of egg of oak silkworm is bred the yellow oophagous trichogrammae of snout moth's larva, belongs to crops biological prevention field.
Background technology
With tussah (
antheraea pernyi) ovum breed the yellow oophagous trichogrammae of snout moth's larva (
trichogramma chilonis) existing method is generally: will not yet by the fresh egg of oak silkworm of parasitism, be cleaned up and using water-soluble thimerosal and carry out after the ovum surface sterilization as the filial generation host egg, using by the egg of oak silkworm of the yellow Trichogramma of snout moth's larva as the parental generation host egg, sprout wings after honeybee and meet honeybee certain hour (24h in darkroom until the yellow oophagous trichogrammae of snout moth's larva in the parental generation ovum, 48h or other), then isolate lower growth of the indoor conditions that the filial generation ovum is placed in natural lighting (bright phase/dark phase be about 14h/10h), the indoor temperature and humidity condition is controlled usually: temperature (25 ± 2) ℃, relative moisture (55 ± 5) %.Under existing mode of reproduction, the yellow oophagous trichogrammae of snout moth's larva breed quality and stability is not good enough, be unfavorable for enlarging the scale of breeding.
Improve the ovum parasitic enemy insect relevant people for synergy and breed in the technology report of quality, the luminous environment regulate and control method that existing 201210347373.7 1 kinds of egg of oak silkworms of patent application are bred trichogramma dendrolimi, only used the luminous environment regulate and control method to carry out synergy; A kind of method with dendrolimus punctatus output ovum breeding Telenomus theophilae of existing patent, a kind of method with madarina using dissected ovum breeding Telenomus theophilae, a kind of method that improves the parasitic dendrolimus punctatus output of Telenomus theophilae percentage of egg parasitism, a kind of method that improves Telenomus theophilae madarina in dissected percentage of egg parasitism, used insect source chemical substance to carry out ovum surface synergy and process.Due to the difference of concrete biological object and biological nature thereof, above method is different from the inventive method.
Summary of the invention
The present invention discloses the synergistic processing method that a kind of egg of oak silkworm is bred the yellow oophagous trichogrammae of snout moth's larva; the surface preparation of use in conjunction host egg and egg parasitoid are grown two kinds of synergy treatment technologies of link luminous environment regulation and control; raising stable egg of oak silkworm are bred the breeding effect of the yellow oophagous trichogrammae of snout moth's larva, strengthen the large-scale production ability of the yellow oophagous trichogrammae biological and ecological methods to prevent plant disease, pests, and erosion of snout moth's larva product.
a kind of egg of oak silkworm disclosed by the invention is bred the synergistic processing method of the yellow oophagous trichogrammae of snout moth's larva, adopts following technical solution:
Under the manual control condition, utilize egg of oak silkworm to breed the yellow oophagous trichogrammae of snout moth's larva, the indoor temperature and humidity condition is controlled and is: temperature (25 ± 2) ℃, relative moisture (55 ± 5) %.Using the egg of oak silkworm of the parasitic yellow oophagous trichogrammae of snout moth's larva as the parental generation ovum, not yet by the fresh egg of oak silkworm of parasitism as the filial generation ovum;
The ovum surface preparation: get cauliflower (
brassica oleracea var. botrytis) the bright body of inflorescence is appropriate, remove surperficial filth strict its rotten part of removing, remaining part is squeezed the juice with juice extractor, through 80 eye mesh screens, filter, obtain bright juice as mother liquor, mother liquor with 20 ~ 25 times of (0.02 ~ 0.03) g/L benzalkonium bromide aqueous solution dilutions as ovum surface preparation solution, fresh tussah cut open the belly ovum through clear water rinsing impurity elimination to after visually observing surperficial cleaning, in this preprocessing solution, soak 4 minutes, take out dry rear as the filial generation ovum for connecing honeybee;
After the yellow oophagous trichogrammae of snout moth's larva honeybee from sprouting wings the parental generation ovum, without under luminous environment by the parental generation ovum with complete the ovum surface preparation after the filial generation ovum with 1: ratio (15-20) is positioned over 24 ~ 48h in same enclosure space, the yellow oophagous trichogrammae queen bee of snout moth's larva i.e. parasitic filial generation ovum in the same space voluntarily, thereby makes the yellow oophagous trichogrammae population augment of snout moth's larva;
Luminous environment is optimized: after parasitic link finishes, remove the yellow oophagous trichogrammae of parental generation ovum and snout moth's larva, retain the filial generation ovum, the yellow oophagous trichogrammae of snout moth's larva that makes to parasitize in the filial generation ovum completes growth, growing link, the natural daylight cycle does not carry out under the regulation and control state, controls egg parasitoid in following luminous environment when the bright phase: the green light of wavelength 475-495nm, intensity of illumination 500-1000lux;
The yellow oophagous trichogrammae of snout moth's larva can continue to repeat above process as kind of a honeybee after completing in egg parasitoid and growing and sprouts wings, makes its population by generation propagation the final oophagous trichogrammae population of acquisition requirement.Ovum surface preparation solution disclosed by the invention is characterized in that making by the following method:
Get the bright body of inflorescence of cauliflower and squeeze the juice, filter through 80 eye mesh screens, obtain bright juice as mother liquor, mother liquor dilutes 20 ~ 25 times and get final product with (0.02 ~ 0.03) g/L benzalkonium bromide aqueous solution.
the advantage that the present invention has with respect to prior art and progress:
In parasitic link, use the vegetable source natural synergistic matter that is easy to obtain in a large number to carry out the host egg surface preparation and improve parasitic effects, to compare and use insect source natural materials to be more suitable for the scale application, cost is lower; In growing link, further improve development quality by egg parasitoid being carried out to the luminous environment regulation and control, be easy to equally realize, with low cost.
Thereby carry out separately the host egg surface preparation, may make host egg that the part grade of fit is lower be improved parasitic rate by parasitic, but can supervene eclosion rate, descend, leave over the problem such as honeybee rate rising and affect final product quality; Though being grown separately the regulation and control of link luminous environment can improve eclosion rate, reduce and leave over the honeybee rate but can't make parasitic rate increase.The inventive method according to the concrete biological nature of the yellow oophagous trichogrammae of snout moth's larva by two kinds of synergy treatment technology use in conjunction, after the ovum surface preparation improves parasitic effects, continuation is stabilized and increased development quality by growing the regulation and control of link luminous environment, thereby obtained, is better than conventional method or adopts separately wherein a certain the breeding effect that Synergistic technique can produce.
Carry out scale and expand when numerous, the every development index of the yellow oophagous trichogrammae of snout moth's larva is better, and stability is stronger.With conventional method, compare, parasitic rate can increase a 4-26 percentage point; The number in the single host egg is moderate, and between sampling, the uniformity coefficient of sample is better; Eclosion rate can increase 0.2-4 percentage point, and the Simultaneous Stabilization improved at parasitic rate is in higher level; Leave over the honeybee rate and reduce 1-3 percentage point, and between sampling, the uniformity coefficient of sample is better.
The accompanying drawing explanation
Fig. 1 is that the present invention breeds general flow chart.
Specific embodiment
embodiment 1:
Ovum surface preparation solution
Get cauliflower (
brassica oleracea var. botrytis) the bright body of inflorescence is appropriate, removes surperficial filth strict its rotten part of removing, remaining part is squeezed the juice with juice extractor, through 80 eye mesh screens, filter, obtain bright juice as mother liquor, mother liquor stirs with 20 ~ 25 times of 0.02 ~ 0.03g/L benzalkonium bromide aqueous solution dilutions, obtains ovum surface preparation solution.
embodiment 2
Low the expansion under numerous ratio, the every index of breeding of application this patent method is in excellent value.
Shown in Fig. 1, under the manual control condition, utilize egg of oak silkworm to breed the yellow oophagous trichogrammae of snout moth's larva, the indoor temperature and humidity condition is controlled as temperature (25 ± 2) ℃, relative moisture (55 ± 5) %.
Get cauliflower (
brassica oleracea var. botrytis) the bright body of inflorescence is appropriate, remove surperficial filth strict its rotten part of removing, remaining part is squeezed the juice with juice extractor, filter through 80 eye mesh screens, obtain bright juice as mother liquor, mother liquor dilutes 20 times as ovum surface preparation solution with the 0.02g/L benzalkonium bromide aqueous solution; Using the egg of oak silkworm of the parasitic yellow oophagous trichogrammae of snout moth's larva as the parental generation ovum, not yet by the fresh egg of oak silkworm of parasitism as the filial generation ovum; Fresh tussah cut open the belly ovum through clear water rinsing impurity elimination to after visually observing surperficial cleaning, be immersed in ovum surface preparation solution 4 minutes, take out dry rear as the filial generation ovum for connecing honeybee.
After the yellow oophagous trichogrammae of snout moth's larva honeybee from sprouting wings the parental generation ovum, without under luminous environment by the parental generation ovum with complete the ovum surface preparation after the filial generation ovum be positioned over 48h in same enclosure space with 1: 15 ratio, the yellow oophagous trichogrammae queen bee of snout moth's larva i.e. parasitic filial generation ovum in the same space voluntarily, thereby makes the yellow oophagous trichogrammae population augment of snout moth's larva;
After parasitic link finishes, remove the yellow oophagous trichogrammae of parental generation ovum and snout moth's larva, retain the filial generation ovum, the yellow oophagous trichogrammae of snout moth's larva that makes to parasitize in the filial generation ovum completes growth, growing link, the natural daylight cycle does not carry out under the regulation and control state, controls egg parasitoid in following luminous environment when the bright phase: the green light of wavelength 475-495nm, intensity of illumination 500-1000lux.
The yellow oophagous trichogrammae of snout moth's larva can continue to repeat above process as kind of a honeybee after completing in egg parasitoid and growing and sprouts wings, makes its population by generation propagation the final oophagous trichogrammae population of acquisition requirement.
When inoculation proportion is low, between each processing method, the breeding effect gap is less, but the every development index of the yellow oophagous trichogrammae of application the inventive method snout moth's larva is better.With conventional method, compare, parasitic rate increases by 4 percentage points; The number in the single host egg is moderate and increase; Eclosion rate increases by 4 percentage points; Leaving over the honeybee rate slightly reduces.Compare with the inventive method, while only carrying out the ovum surface preparation, have the number in the single host egg, leave over the higher problem of honeybee rate; When only luminous environment is grown in regulation and control, though can improve development index, can't make parasitic rate increase.Concrete effect is in Table 1.
under the different mating systems of table 1, every index of breeding contrasts (the parental generation ovum: the filial generation ovum is 1: 15)
| Process | Parasitic rate (%) | The number in the single host egg (head) | Eclosion rate (%) | Leave over honeybee rate (%) |
| The present invention | 90.75±0.66a | 96.50±2.46ab | 94.25±0.66a | 15.90±1.95a |
| Conventional method | 86.75±1.83ab | 88.40±6.27b | 89.99±0.94b | 16.21±3.56a |
| Only ovum surface preparation | 90.75±1.02a | 106.67±3.38a | 94.03±1.07a | 19.68±1.60a |
| Only luminous environment is grown in regulation and control | 85.75±0.86b | 91.00±1.75b | 94.50±0.67a | 14.41±2.34a |
In table, data are mean value ± standard error, with indicating different lowercase alphabet differentials after column data, different significantly (parasitic rate, eclosion rate, single ovum are left over the honeybee rate and are adopted the conversion of arcsine square root for P<0.05, Tukey method, the number in the single host egg adopts the square root conversion), lower same.
embodiment 3
Under the numerous ratio of medium expansion, the every index of breeding of application this patent method still can be stable at the excellent value zone.
Shown in Fig. 1, under the manual control condition, utilize egg of oak silkworm to breed the yellow oophagous trichogrammae of snout moth's larva, the indoor temperature and humidity condition is controlled as temperature (25 ± 2) ℃, relative moisture (55 ± 5) %.Using the egg of oak silkworm of the parasitic yellow oophagous trichogrammae of snout moth's larva as the parental generation ovum, not yet by the fresh egg of oak silkworm of parasitism as the filial generation ovum;
Get cauliflower (
brassica oleracea var. botrytis) the bright body of inflorescence is appropriate, remove surperficial filth strict its rotten part of removing, remaining part is squeezed the juice with juice extractor, through 80 eye mesh screens, filter, obtain bright juice as mother liquor, mother liquor with 25 times of 0.03g/L benzalkonium bromide aqueous solution dilutions as ovum surface preparation solution, fresh tussah cut open the belly ovum through clear water rinsing impurity elimination to after visually observing surperficial cleaning, with this preprocessing solution, soak 4 minutes, take out dry rear as the filial generation ovum for connecing honeybee;
After the yellow oophagous trichogrammae of snout moth's larva honeybee from sprouting wings the parental generation ovum, without under luminous environment by the parental generation ovum with complete the ovum surface preparation after the filial generation ovum be positioned over 48h in same enclosure space with 1: 20 ratio, the yellow oophagous trichogrammae queen bee of snout moth's larva i.e. parasitic filial generation ovum in the same space voluntarily, thereby makes the yellow oophagous trichogrammae population augment of snout moth's larva;
After parasitic link finishes, remove the yellow oophagous trichogrammae of parental generation ovum and snout moth's larva, retain the filial generation ovum, the yellow oophagous trichogrammae of snout moth's larva that makes to parasitize in the filial generation ovum completes growth, growing link, the natural daylight cycle does not carry out under the regulation and control state, controls egg parasitoid in following luminous environment when the bright phase: the green light of wavelength 475-495nm, intensity of illumination 500-1000lux; The yellow oophagous trichogrammae of snout moth's larva can continue to repeat above process as kind of a honeybee after completing in egg parasitoid and growing and sprouts wings, makes its population by generation propagation the final oophagous trichogrammae population of acquisition requirement.
After inoculation proportion improves, between each processing method, the breeding effect gap widens, and the every development index of the yellow oophagous trichogrammae of application the inventive method snout moth's larva is better, and stability significantly strengthens.With conventional method, compare, parasitic rate can increase by 26 percentage points; The number in the single host egg there was no significant difference; It is stable that eclosion rate keeps, and because parasitic rate significantly improves, do not decrease; Leave over the honeybee rate and reduce by 3 percentage points, and between sampling, the uniformity coefficient of sample is better.Compare with the inventive method, while only carrying out the ovum surface preparation, have the eclosion rate downward trend; When only luminous environment is grown in regulation and control, though can improve development index, can't make parasitic rate increase.Concrete effect is in Table 2.
under the different mating systems of table 2, every index of breeding contrasts (the parental generation ovum: the filial generation ovum is 1: 20)
| Process | Parasitic rate (%) | The number in the single host egg (head) | Eclosion rate (%) | Leave over honeybee rate (%) |
| The present invention | 88.00±1.38a | 76.88±4.40a | 90.75±0.80ab | 23.50±0.76a |
| Conventional method | 62.00±2.88b | 81.00±7.37a | 90.53±1.30ab | 27.18±2.68a |
| Only ovum surface preparation | 86.25±2.29a | 75.83±4.87a | 89.13±1.53b | 24.39±1.25a |
| Only luminous environment is grown in regulation and control | 62.75±1.98b | 80.25±3.96a | 94.00±0.63a | 21.13±1.51a |
Claims (2)
1. an egg of oak silkworm is bred the synergistic processing method of the yellow oophagous trichogrammae of snout moth's larva, comprises the following steps:
Under the manual control condition, utilize egg of oak silkworm to breed the yellow oophagous trichogrammae of snout moth's larva, the indoor temperature and humidity condition is controlled and is: temperature (25 ± 2) ℃, relative moisture (55 ± 5) %; Using the egg of oak silkworm of the parasitic yellow oophagous trichogrammae of snout moth's larva as the parental generation ovum, not yet by the fresh egg of oak silkworm of parasitism as the filial generation ovum;
The ovum surface preparation: the bright body of inflorescence of getting cauliflower is squeezed the juice, and through 80 eye mesh screens, filters, and obtains bright juice as mother liquor, and mother liquor dilutes 20 ~ 25 times as ovum surface preparation solution with (0.02 ~ 0.03) g/L benzalkonium bromide aqueous solution;
Fresh tussah cut open the belly ovum through clear water rinsing impurity elimination to after visually observing surperficial cleaning, be immersed in ovum surface preparation solution, take out dry rear as the filial generation ovum for connecing honeybee;
After the yellow oophagous trichogrammae of snout moth's larva honeybee from sprouting wings the parental generation ovum, without under luminous environment by the parental generation ovum with complete the ovum surface preparation after the filial generation ovum with 1: ratio (15-20) is positioned in same enclosure space, the yellow oophagous trichogrammae queen bee of snout moth's larva i.e. parasitic filial generation ovum in the same space voluntarily, thereby makes the yellow oophagous trichogrammae population augment of snout moth's larva;
Luminous environment is optimized: after parasitic link finishes, remove the yellow oophagous trichogrammae of parental generation ovum and snout moth's larva, retain the filial generation ovum, the yellow oophagous trichogrammae of snout moth's larva that makes to parasitize in the filial generation ovum completes growth, is growing link, and the natural daylight cycle does not carry out under the regulation and control state; Control egg parasitoid in following luminous environment when the bright phase: the green light of wavelength 475-495nm, intensity of illumination 500-1000lux;
The yellow oophagous trichogrammae of snout moth's larva can continue to repeat above process as kind of a honeybee after completing in egg parasitoid and growing and sprouts wings, makes its population by generation propagation the final oophagous trichogrammae population of acquisition requirement.
2. an ovum surface preparation solution is characterized in that making by the following method:
Get the bright body of inflorescence of cauliflower and squeeze the juice, filter through 80 eye mesh screens, obtain bright juice as mother liquor, mother liquor stirs and get final product with 20 ~ 25 times of (0.02 ~ 0.03) g/L benzalkonium bromide aqueous solution dilutions.
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| CN105145487A (en) * | 2014-07-23 | 2015-12-16 | 中国科学院动物研究所 | Trichogramma japonicun ashmead artifical breeding production method |
| CN106973866A (en) * | 2017-05-17 | 2017-07-25 | 中国林业科学研究院森林生态环境与保护研究所 | A kind of artificial breeding method of east anstatus and the application in preventing and treating forest pest |
| CN105766803B (en) * | 2016-03-15 | 2018-04-27 | 吉林农业大学 | Egg of oak silkworm mixes breeding trichogramma dendrolimi and Trichogramma Ostriniae method for host |
| CN112913785A (en) * | 2021-01-29 | 2021-06-08 | 贾自峰 | Method for improving parasitic rate of parasitic tenebrio molitor of dastarcus helophoroides by using longicorn soaking solution |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105145487A (en) * | 2014-07-23 | 2015-12-16 | 中国科学院动物研究所 | Trichogramma japonicun ashmead artifical breeding production method |
| CN105766803B (en) * | 2016-03-15 | 2018-04-27 | 吉林农业大学 | Egg of oak silkworm mixes breeding trichogramma dendrolimi and Trichogramma Ostriniae method for host |
| CN106973866A (en) * | 2017-05-17 | 2017-07-25 | 中国林业科学研究院森林生态环境与保护研究所 | A kind of artificial breeding method of east anstatus and the application in preventing and treating forest pest |
| CN112913785A (en) * | 2021-01-29 | 2021-06-08 | 贾自峰 | Method for improving parasitic rate of parasitic tenebrio molitor of dastarcus helophoroides by using longicorn soaking solution |
| CN112913785B (en) * | 2021-01-29 | 2022-08-02 | 贾自峰 | Method for improving parasitic rate of parasitic tenebrio molitor of dastarcus helophoroides by using longicorn soaking solution |
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| Publication number | Publication date |
|---|---|
| CN103444633B (en) | 2014-11-26 |
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