CN112913785B - Method for improving parasitic rate of parasitic tenebrio molitor of dastarcus helophoroides by using longicorn soaking solution - Google Patents
Method for improving parasitic rate of parasitic tenebrio molitor of dastarcus helophoroides by using longicorn soaking solution Download PDFInfo
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- 241000254109 Tenebrio molitor Species 0.000 title claims abstract description 75
- 230000003071 parasitic effect Effects 0.000 title claims abstract description 58
- 241000164724 Dastarcus helophoroides Species 0.000 title claims abstract description 56
- 238000002791 soaking Methods 0.000 title claims abstract description 41
- 238000000034 method Methods 0.000 title claims abstract description 28
- 241001481710 Cerambycidae Species 0.000 title claims description 18
- 238000011081 inoculation Methods 0.000 claims abstract description 20
- 239000000243 solution Substances 0.000 claims abstract description 18
- 241001609695 Anoplophora glabripennis Species 0.000 claims abstract description 15
- 235000013601 eggs Nutrition 0.000 claims abstract description 13
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 12
- 238000001035 drying Methods 0.000 claims abstract description 9
- 239000011259 mixed solution Substances 0.000 claims abstract description 9
- 229920000742 Cotton Polymers 0.000 claims abstract description 6
- 239000012153 distilled water Substances 0.000 claims abstract description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 6
- 241000382353 Pupa Species 0.000 claims description 15
- 239000007788 liquid Substances 0.000 claims description 14
- 230000032669 eclosion Effects 0.000 claims description 10
- FFRBMBIXVSCUFS-UHFFFAOYSA-N 2,4-dinitro-1-naphthol Chemical group C1=CC=C2C(O)=C([N+]([O-])=O)C=C([N+]([O-])=O)C2=C1 FFRBMBIXVSCUFS-UHFFFAOYSA-N 0.000 claims description 6
- 241000254173 Coleoptera Species 0.000 claims description 5
- 238000009395 breeding Methods 0.000 abstract description 4
- 230000001488 breeding effect Effects 0.000 abstract description 4
- 241000238631 Hexapoda Species 0.000 description 4
- 241000209219 Hordeum Species 0.000 description 3
- 235000007340 Hordeum vulgare Nutrition 0.000 description 3
- 241000607479 Yersinia pestis Species 0.000 description 3
- 235000016709 nutrition Nutrition 0.000 description 3
- 230000035764 nutrition Effects 0.000 description 3
- 241001484304 Bothrideridae Species 0.000 description 2
- 230000000052 comparative effect Effects 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 230000024241 parasitism Effects 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 239000013589 supplement Substances 0.000 description 2
- 241000283690 Bos taurus Species 0.000 description 1
- 241001442207 Monochamus alternatus Species 0.000 description 1
- 240000000249 Morus alba Species 0.000 description 1
- 235000008708 Morus alba Nutrition 0.000 description 1
- 241000122904 Mucuna Species 0.000 description 1
- 241000758706 Piperaceae Species 0.000 description 1
- 241000555745 Sciuridae Species 0.000 description 1
- 241001414987 Strepsiptera Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000004806 packaging method and process Methods 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000007306 turnover Effects 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a method for improving parasitic rate of parasitic tenebrio molitor of dastarcus helophoroides by using longhorn soaking solution, which comprises the following steps: (1) selecting healthy five-instar anoplophora glabripennis larvae without wound and pollution on body surface, and slowly baking in a culture drying box; (2) soaking the anoplophora glabripennis larvae treated in the step (1) in alcohol, and then adding distilled water to obtain a mixed solution for later use; (3) soaking the yellow mealworm pupae to be inoculated in the prepared mixed solution, taking out the yellow mealworm pupae from the mixed solution, putting the yellow mealworm pupae on medical absorbent cotton, airing the yellow mealworm pupae, and putting the yellow mealworm pupae into an inoculation tube for inoculation; (4) inoculating the dastarcus helophoroides eggs to the aired yellow mealworm pupae according to a certain ratio of eggs to pupae, adding the yellow mealworm pupae soaked in the step (3) into each inoculation tube every 5 days, continuously adding for 2-3 times, and observing the result after inoculation. The method improves parasitic rate and imago emergence rate of dastarcus helophoroides to 100%, saves labor, and reduces breeding cost of dastarcus helophoroides.
Description
Technical Field
The invention belongs to the technical field of methods for improving the parasitic rate of parasitic natural enemy insects, and particularly relates to a method for improving the parasitic rate of parasitic tenebrio molitor of dastarcus helophoroides by using a longicorn soaking solution.
Background
The Dastarculia helophoroides Fairmaire is originally belonging to Coleoptera (Coleoptera) Piperaceae (Colydidae) and independently becomes insects of Bothrideridae (Bothrideridae), namely Dastacia firma, Dastarcus helophoroides, Mucuna parasitides and Sinomenii parasitides. The dastarcus helophoroides is distributed in the range of 105-135 degrees of east longitude and 30-40 degrees of north latitude, and is known to be peculiar to China and Japan. It is known that 18 provinces such as Liaoning, Shanghai, Shandong, Jiangsu, Guangdong and Sichuan are distributed in China. Japan is distributed mainly in japan, kyu, and osaka. The dastarcus helophoroides is one of the main natural enemies of the natural cattle pest insects which attack trunk.
In agriculture and forestry production in China, longhorn beetles are a very serious harmful pest, and include common species such as monochamus alternatus hope, anoplophora glabripennis, marmot longicorn, mulberry longicorn and the like. Most of the adults can gnaw the tender branch bark of the tree to supplement nutrition, so that the host is weakened; the larvae bore the trunks of trees in large quantities, causing the trees to become pieces, turn over and die. Because the longicorn pests live in extremely hidden places and live in trunks in most of the life, effective measures are extremely difficult to take for prevention and treatment in actual production. The natural enemy insects such as the dastarcus helophoroides and the like are released, so that the control problem of the longicorn can be better solved.
As dastarcus helophoroides is a parasitic insect, the artificial inoculation of the alternative host is mainly adopted for propagation in China at present, and the propagation method generally goes through the following three processes: alternative host culture-artificial inoculation-package culture. In this mode, a large amount of energy and labor are consumed in the processes of alternative host culture, artificial inoculation and packaging, the working efficiency is extremely low, and the propagation cost is high.
At present, the barley pupa is a commonly used alternative host for breeding the dastarcus helophoroides, but the breeding cost of the barley pupa is high, the seasonality is strong, and the rotting rate in summer is high, so that the parasitism rate of the dastarcus helophoroides in summer is low. Tenebrio molitor can be bred all the year round, the cost is low, the nutrition is similar to that of barley worms, and the Tenebrio molitor pupa is used as a substitute host to improve the parasitic effect. Therefore, the invention discloses a method for improving the parasitic rate of the parasitic tenebrio molitor of the dastarcus helophoroides by using the longicorn soaking solution, and the method can improve the parasitic rate and the eclosion rate of the parasitic tenebrio molitor of the dastarcus helophoroides to be equivalent to or even higher than the parasitic rate and the eclosion rate of the parasitic tenebrio molitor of the dastarcus helophoroides.
Disclosure of Invention
In order to solve the defects in the prior art, the invention aims to provide a method for improving the parasitic rate of parasitic tenebrio molitor of dastarcus helophoroides by using a longhorn soaking solution. The method has high parasitism rate, high eclosion rate of imago and low propagation cost.
In order to achieve the purpose, the invention adopts the following technical scheme:
a method for improving parasitic rate of parasitic tenebrio molitor of dastarcus helophoroides by using longicorn soaking solution comprises the following steps:
(1) selecting healthy five-instar anoplophora glabripennis larvae without wound and pollution on body surface, placing into a culture drying box, and slowly drying until the surface of the larvae is golden yellow for later use;
(2) soaking the anoplophora glabripennis larvae which are baked to be golden yellow in the step (1) in alcohol, and then adding distilled water to obtain a mixed solution for later use;
(3) soaking the yellow mealworm pupae to be inoculated in the mixed liquid prepared in the step (2), taking out the yellow mealworm pupae from the mixed liquid, putting the yellow mealworm pupae on medical absorbent cotton, airing the yellow mealworm pupae, and putting the yellow mealworm pupae into an inoculation tube for inoculation; the concentration of the mixed liquid for soaking the yellow mealworm pupae is not suitable to be too high, and if the concentration of the mixed liquid is too high, the yellow mealworm pupae can be over-nourished and rotten, so that the parasitic rate and the eclosion rate of the parasitic yellow mealworms of the dastarcus helophoroides are reduced.
(4) Inoculating the eggs of the dastarcus helophoroides to the yellow mealworm pupas aired in the step (3) according to a certain ratio of the eggs to the pupas, adding the yellow mealworm pupas soaked by the mixed liquid in the step (3) into each inoculation tube every 5 days to supplement nutrition for the dastarcus helophoroides larvae, continuously adding for 2-3 times, and observing the result after inoculation.
Further, the temperature of the culture drying box in the step (1) is 40-60 ℃.
Further, the using amount of the alcohol in the step (2) is 3ml, and the volume concentration is 75%; the amount of the distilled water used is 0.4-0.8 ml. The purpose of soaking the anoplophora glabripennis larvae with the alcohol is to effectively reduce the rotting rate of the anoplophora glabripennis pupa body, more effectively separate and extract required liquid and smell from the anoplophora glabripennis pupa body, and the alcohol can also sterilize to prevent the anoplophora glabripennis pupa body from mildewing.
Further, the soaking time of the longicorn larvae in the alcohol in the step (2) is 14 to 16 days.
Further, the time for soaking the yellow mealworm pupae in the mixed liquid in the step (3) is 25-35 min.
Further, the yellow mealworm pupae in the step (3) are placed in medical absorbent cotton and dried for 8-12 min.
Further, the ratio of the eggs to the pupae in the step (4) is 4: 1.
Further, the number of the yellow mealworm pupae soaked by the mixed liquid is 1-2 in each inoculation wanting to be added into the inoculation tube in the step (4).
Further, the dastarcus helophoroides eggs of the dastarcus helophoroides in the step (4) are inoculated to the yellow mealworm pupae on day 15 for cocooning, and the dastarcus helophoroides on day 19 is further carried out in the operating environment of 23-26 ℃ at room temperature and 60-75% of humidity.
Compared with the prior art, the invention has the following beneficial effects: according to the method, the yellow mealworm pupae to be inoculated are placed into the alcohol mixed liquor soaked in the anoplophora glabripennis and soaked for 25-35min, so that the parasitic rate of the parasitic yellow mealworms of the dastarcus helophoroides is increased to 100%, the eclosion rate of adults is increased to 100%, labor is saved, and the propagation cost of the parasitic yellow mealworms of the dastarcus helophoroides is reduced.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and do not limit the invention.
Example 1
A method for improving parasitic rate of parasitic tenebrio molitor of dastarcus helophoroides by using longicorn soaking solution comprises the following steps:
(1) selecting healthy five-instar anoplophora glabripennis larvae without wound and pollution on body surface, placing into a culture drying box with the temperature set to 40-60 ℃, and slowly drying until the surface of the larvae is golden yellow for later use;
(2) soaking the anoplophora glabripennis larvae which are baked to be golden yellow in 3ml of 75% alcohol with volume concentration for 14-16 days, and then adding 0.4-0.8ml of distilled water to obtain a mixed solution for later use;
(3) soaking the yellow mealworm pupae to be inoculated in the mixed liquid prepared in the step (2) for 25-35min, taking out the yellow mealworm pupae from the mixed liquid, airing on medical absorbent cotton for 8-12min, and filling into an inoculation tube for inoculation;
(4) inoculating the eggs of the dastarcus helophoroides to the yellow mealworm pupas aired in the step (3) according to the ratio of the eggs to the pupas of the yellow mealworm to be 4:1, adding 1-2 yellow mealworm pupas soaked by the mixed liquid in the step (3) into each inoculation tube every 5 days, continuously adding for 2-3 times, observing that the 15 th day dastarcus helophoroides, in which the eggs of the dastarcus helophoroides are inoculated to the yellow mealworm pupas, begin to cocoon, the 19 th day dastarcus helophoroides adult begins to eclosite, and the parasitizing rate and the eclosion rate of the yellow mealworm of the dastarcus helophoroides are both 100%. All the operations are carried out at room temperature of 23-26 ℃ and humidity of 60-75%.
Comparative example 1
In the prior art, the method for improving the parasitic rate of the yellow mealworms parasitized on the dastarcus helophoroides adopts yellow mealworms which are not treated at all to breed the dastarcus helophoroides, and the other operations are the same as example 1.
Test examples
The example 1 of the invention is used as a soaking group, the comparative example 1 is used as a blank control group (non-soaking group), 10 yellow mealworm breeding dastarcus helophoroides test objects are respectively picked from each of the soaking group and the non-soaking group under the same test conditions, the test is repeated for 4 times, the parasitic number of the dastarcus helophoroides of each test soaking group and the non-soaking group, the eclosion number of the dastarcus helophoroides, and the parasitic rate and the eclosion rate of the dastarcus helophoroides corresponding to the test soaking group and the non-soaking group are observed, and the detailed data of each test are shown in the table 1.
TABLE 1 parasitic rate and adult eclosion rate of dastarcus helophoroides parasitic yellow mealworms of the soaked group and the non-soaked group
As can be seen from the data in table 1, compared with the non-soaked group, the soaking group of the present invention can significantly increase the parasitic rate and the adult emergence rate of the parasitic tenebrio molitor dastarcus helophoroides by soaking the pupae of the tenebrio molitor to be inoculated in the alcohol mixed solution soaked in the anoplophora glabripennis for 25-35min, and both the parasitic rate and the adult emergence rate of the parasitic tenebrio molitor dastarcus helophoroides are increased to 100%.
The foregoing is directed to embodiments of the present invention, and it is understood that various changes and modifications may be made by those skilled in the art without departing from the scope and spirit of the invention. The scope of the claims of the present application shall be determined by the contents of the claims, and the description of the embodiments and the like in the specification shall be used to explain the contents of the claims.
Claims (10)
1. A method for improving parasitic rate of parasitic tenebrio molitor of dastarcus helophoroides by using longhorn beetle soak solution is characterized by comprising the following steps:
(1) selecting healthy five-instar anoplophora glabripennis larvae without wound and pollution on body surface, placing into a culture drying box, and slowly drying until the surface of the larvae is golden yellow for later use;
(2) soaking the anoplophora glabripennis larvae which are baked to be golden yellow in the step (1) in alcohol, and then adding distilled water to obtain a mixed solution for later use;
(3) soaking the yellow mealworm pupae to be inoculated in the mixed liquid prepared in the step (2), taking out the yellow mealworm pupae from the mixed liquid, putting the yellow mealworm pupae on medical absorbent cotton, airing the yellow mealworm pupae, and putting the yellow mealworm pupae into an inoculation tube for inoculation;
(4) inoculating the eggs of the parasitic yellow mealworms of the dastarcus helophoroides to the yellow mealworms aired in the step (3) according to a certain ratio of the eggs to the pupae, adding the yellow mealworms soaked in the mixed liquid in the step (3) into each inoculation tube every 5 days, continuously adding for 2-3 times, and observing the result after inoculation.
2. The method for improving the parasitic rate of the dastarcus helophoroides and the yellow mealworms by using the longicorn soaking solution as claimed in claim 1, wherein the temperature of the culture drying oven in the step (1) is 40-60 ℃.
3. The method for improving the parasitic rate of the dastarcus helophoroides and the yellow mealworms by using the longicorn soaking solution as claimed in claim 1, wherein the amount of the alcohol used in the step (2) is 3ml, and the volume concentration is 75%; the amount of the distilled water used is 0.4-0.8 ml.
4. The method for improving the parasitic rate of the dastarcus helophoroides yellow mealworm by using the longhorn soaking solution according to claim 1, wherein the soaking time of the longhorn larvae in the alcohol in the step (2) is 14 to 16 days.
5. The method for improving the parasitic rate of the parasitic tenebrio molitor of the dastarcus helophoroides by using the longicorn soaking solution as claimed in claim 1, wherein the time for soaking the tenebrio molitor pupae in the mixed solution in the step (3) is 25-35 min.
6. The method for improving the parasitic rate of the parasitic tenebrio molitor of the dastarcus helophoroides by using the longicorn soaking solution according to claim 1, wherein the airing time of the tenebrio molitor pupae in the step (3) in the medical absorbent cotton is 8-12 min.
7. The method for improving the parasitic rate of the parasitic tenebrio molitor of the dastarcus helophoroides by using the longicorn soaking solution as claimed in claim 1, wherein the initial ratio of egg to pupa in the step (4) is 4: 1.
8. The method for improving the parasitic rate of the parasitic tenebrio molitor of the dastarcus helophoroides by using the longicorn soaking solution according to claim 1, wherein the number of the tenebrio molitor pupae soaked by the mixed solution is 1-2 in each adding of the inoculation tube in the step (4).
9. The method for improving parasitic rate of parasitic tenebrio molitor of dastarcus helophoroides by using longhorn beetle soaking solution according to claim 1, wherein eggs of parasitic tenebrio molitor of dastarcus helophoroides in step (4) are inoculated to the pupa of tenebrio molitor, the parasitic tenebrio molitor of dastarcus helophoroides is cocooned 15 th, the adult tenebrio molitor of dastarcus helophoroides 19 th is eclosized, and the parasitic rate and the eclosion rate are both 100%.
10. The method for improving the parasitic rate of the dastarcus helophoroides and the yellow mealworms by using the longicorn soaking solution according to any one of claims 1 to 9, wherein the operating environment of the method is 23 to 26 ℃ at room temperature and 60 to 75% of humidity.
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