CN108935334A - A kind of method of dastarcus helophoroides breeding host's sterilization treatment - Google Patents
A kind of method of dastarcus helophoroides breeding host's sterilization treatment Download PDFInfo
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- CN108935334A CN108935334A CN201810652834.9A CN201810652834A CN108935334A CN 108935334 A CN108935334 A CN 108935334A CN 201810652834 A CN201810652834 A CN 201810652834A CN 108935334 A CN108935334 A CN 108935334A
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- 230000001954 sterilising effect Effects 0.000 title claims abstract description 75
- 238000004659 sterilization and disinfection Methods 0.000 title claims abstract description 47
- 241000164724 Dastarcus helophoroides Species 0.000 title claims abstract description 27
- 238000011282 treatment Methods 0.000 title claims abstract description 24
- 230000001488 breeding effect Effects 0.000 title claims abstract description 18
- 238000009395 breeding Methods 0.000 title claims abstract description 17
- 238000000034 method Methods 0.000 title claims abstract description 17
- 241001481710 Cerambycidae Species 0.000 claims abstract description 14
- 238000011081 inoculation Methods 0.000 claims abstract description 6
- 238000011109 contamination Methods 0.000 claims abstract description 4
- 241000894006 Bacteria Species 0.000 claims description 6
- 241001442207 Monochamus alternatus Species 0.000 claims description 6
- 239000000463 material Substances 0.000 claims description 4
- 238000007664 blowing Methods 0.000 claims description 3
- 239000002775 capsule Substances 0.000 claims description 3
- 230000000630 rising effect Effects 0.000 claims 1
- 238000012545 processing Methods 0.000 abstract description 12
- 230000003071 parasitic effect Effects 0.000 abstract description 5
- 230000003247 decreasing effect Effects 0.000 abstract 1
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 7
- 241000238631 Hexapoda Species 0.000 description 5
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 4
- 239000011229 interlayer Substances 0.000 description 4
- 238000012216 screening Methods 0.000 description 4
- 238000010586 diagram Methods 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 229910001220 stainless steel Inorganic materials 0.000 description 3
- 239000010935 stainless steel Substances 0.000 description 3
- 241000254173 Coleoptera Species 0.000 description 2
- 241000558585 Colydiinae Species 0.000 description 2
- 241000607479 Yersinia pestis Species 0.000 description 2
- 208000015181 infectious disease Diseases 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 238000003672 processing method Methods 0.000 description 2
- 230000005855 radiation Effects 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- 241001609695 Anoplophora glabripennis Species 0.000 description 1
- 241001042196 Batocera horsfieldi Species 0.000 description 1
- 241000283725 Bos Species 0.000 description 1
- 241001484304 Bothrideridae Species 0.000 description 1
- 241000164722 Dastarcus Species 0.000 description 1
- 241000172741 Dorcaschema wildii Species 0.000 description 1
- 235000009161 Espostoa lanata Nutrition 0.000 description 1
- 240000001624 Espostoa lanata Species 0.000 description 1
- 241001074085 Scophthalmus aquosus Species 0.000 description 1
- 241001136820 Xylocopa virginica Species 0.000 description 1
- 230000001476 alcoholic effect Effects 0.000 description 1
- 239000012491 analyte Substances 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 230000009514 concussion Effects 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000013332 literature search Methods 0.000 description 1
- 230000024241 parasitism Effects 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 230000027272 reproductive process Effects 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 239000008223 sterile water Substances 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Apparatus For Disinfection Or Sterilisation (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a kind of methods of dastarcus helophoroides breeding host's sterilization treatment.This method comprises: (1) selection body surface does not have wound, free of contamination longicorn mature larva；(2) roundheaded borer screened in step (1) is respectively charged into worm box, every lattice one are mutually isolated, then worm box is put into sterilizing frame；(3) baking oven is arranged to 60 DEG C, is preheating to set temperature, sterilizing frame is put into baking oven, to host in sterilizing under the conditions of 60 DEG C, sterilization time is 25~35 minutes；(4) it by the worm box after sterilization treatment in step (3), is put between inoculation, 25 DEG C of room temperatures are risen again 30-60 minutes.The method of the present invention carries out living body sterilization treatment, and host's body surface germ substantially reduces after sterilizing, and polypide clump count, Final pollution rate are remarkably decreased, simultaneously, host polypide softens after sterilization treatment, improves parasitic rate and breeding efficiency, hence it is evident that be better than conventional sterilization processing mode.
Description
Technical field
The invention belongs to dastarcus helophoroides to breed field, be related to a kind of method of dastarcus helophoroides breeding host's sterilization treatment.
Background technique
Dastarcus helophoroides (Dastarcus helophoroides Fairmaire) original is under the jurisdiction of coleoptera (Coleoptera)
Colydiidae (Colydiidae), it is rear independent to become Xue Jia section (Bothrideridae) insect, alias Dastarcus helophoroides, Dastarcus helophorides,
Carpenter bee post first and hang wing post it is first-class.Dastarcus helophoroides are distributed in the range of 105 °~135 ° of east longitude, 30 °~40 ° of north latitude, it is known that
China and institute of Japan are peculiar.It is distributed known to domestic in 18 provinces and cities such as Liaoning, Shanghai, Shandong, Jiangsu, Guangdong, Sichuan.Japan
It is distributed mainly on Honshu, nine divisions of China in remote antiquity and Osaka.Dastarcus helophoroides are one of the Natural Enemies insects of longicorn class trunk borer.
In China's agricultural and forestry production, longicorn be it is a kind of endanger extremely serious pest, including Monochamus alternatus Hope, anoplophora glabripennis,
The frequent species such as mountain longicorn, batocera horsfieldi, mulberry borer.Their adult mostly to gnaw trees spray skin extra-nutrition, is caused
Host is weak；The a large amount of brill moth trunks of larva cause trees to roll in flakes, are dead.Since the life of longicorn class pest is extremely hidden, one
Raw most of time is lived in trunk, therefore extremely difficult adopt an effective measure is prevented and treated in actual production.Flower suede is discharged to post
First-class natural enemy insect can preferably solve the problems, such as the prevention and treatment of longicorn.
Natural enemy dastarcus helophoroides mainly take artificial breeding mode at present, that is, utilize Hosts (Monochamus alternatus Hope) artificial infection
It is bred.In reproductive process, the sterilization treatment of host be reduce germ infection and improve breeding efficiency important means and
Necessary links.The mode of ultraviolet irradiation or 75% alcohol washes is mainly taken in traditional host's sterilizing, and sterilization effect is poor, easily
The problems such as causing Final pollution seriously affects the breeding efficiency of dastarcus helophoroides.The high-temperature sterilization processing method of living body host sterilizes
Effect is good, and host body surface germ substantially reduces, polypide softens, and inoculation efficiency significantly improves；By literature search, domestic not yet to appear in the newspapers
Road.
Summary of the invention
In view of the deficiencies of the prior art, it is an object of the present invention to provide the sterilized places of dastarcus helophoroides breeding host a kind of
Reason method.
The purpose of the present invention can be achieved through the following technical solutions:
A kind of method of dastarcus helophoroides breeding host's sterilization treatment, comprising the following steps:
(1) select worm: selection body surface does not have wound, free of contamination healthy dastarcus helophoroides breeding host longicorn mature larva；
(2) mounted box: the roundheaded borer screened in step (1) is respectively charged into worm box, and every lattice one are mutually isolated, then
Worm box is put into sterilizing frame；
(3) it sterilizes: baking oven being arranged to 60 DEG C, is preheating to set temperature, ready sterilizing frame in step (2) is put into
In baking oven, sterilizing frame baffle side avoids hot wind from directly blowing to worm box air outlet, to host in going out under the conditions of 60 DEG C
Bacterium, sterilization time are 25~35 minutes；
(4) it rises again: the worm box after sterilization treatment in step (3) is put between inoculation, 25 DEG C of room temperatures are risen again 30-60 minutes.
As the preferred of the method for the present invention, sterilization container described in step (2) is homemade " polypide sterilization cassette ", and material is
Transparent heat resistant plastic is made of upper cover (1) and lower cover (2) two parts, the recess of 50 1 × 3.5cm is dispersed on upper and lower cover
Zooecium (3), with after, upper and lower zooecium merges to form 50 mutually isolated " capsule " zooeciums lid.
The dastarcus helophoroides breed the preferred Monochamus alternatus Hope of host longicorn.
Sterilization time preferably 30 minutes.
It rises again the time preferably 60 minutes.
The utility model has the advantages that
The present invention takes the mode of high-temperature sterilization to substitute conventional sterilization mode, take specific sterilization time, sterilising temp,
Time control rise again to dastarcus helophoroides host --- Monochamus alternatus Hope larva carries out living body sterilization treatment, host's body surface germ after sterilizing
It substantially reduces, polypide clump count has dropped 87.1%, and Final pollution rate has dropped 86.9%；Meanwhile host insect after sterilization treatment
Body softening, conducive to the parasitism intrusion of dastarcus helophoroides larva, parasitic rate improves 10%, breeds efficiency (dastarcus helophoroides imago output
Rate) improve 47.4%, hence it is evident that it is better than conventional sterilization processing mode.As the preferred embodiment of the present invention, using special
Polypide can be isolated and be conducive to be improved sterilization effect by thermal balance by sterilization cassette.
Detailed description of the invention
Fig. 1 makes sterilization cassette structural schematic diagram by oneself
1 is upper cover, and 2 be lower cover, and 3 be recess zooecium.
Fig. 2 sterilizing frame structural schematic diagram
4 be supporting baffle, and 5 be interlayer baffle.
Specific embodiment
Embodiment 1
Polypide sterilization cassette is self-control sterilizing installation, is mainly made of worm box and sterilizing frame two parts, structural schematic diagram such as Fig. 1
It is shown.
Worm box material be heat resistant plastice, be made of upper cover 1 and 2 two parts of lower cover, be dispersed in upper and lower covers 50 1 ×
The recess zooecium 3 of 3.5cm, with after, upper and lower zooecium merges to form 50 mutually isolated " capsule " zooeciums lid.By day subject to sterilization
Ox larva is individually put into these zooeciums, and polypide can be made mutually isolated, and reduction, which cuts one another's throat, causes polypide wound with autotomy, reduces dirty
Contaminate probability.
Sterilizing frame material is stainless steel, forms (Fig. 2) by supporting baffle (4) and interlayer baffle (5).Supporting baffle is thickness
The stainless steel plate of 0.2cm, interlayer baffle are 1cm bore diameter stainless steel net.Sterilizing frame is by 3 pieces of supporting baffles (two sides+bottom plate) and 9 pieces
Interlayer baffle is welded to form.
When sterilizing, host longicorn is packed into worm box, sterilizing frame is put into, places into baking oven.Pay attention to baffle-panels to baking oven outlet air
Mouthful, avoid hot wind from directly blowing to worm box.
Embodiment 2
Dastarcus helophoroides breed host's difference Biocidal treatment method and compare application example:
(1) select worm: selection body surface does not have wound, free of contamination healthy Monochamus alternatus Hope mature larva 2500；
(2) processing method: to above-mentioned longicorn take respectively high-temperature sterilization, the sterilizing of 75% alcohol, ultraviolet lamp radiation sterilization,
5 processing modes such as the sterilizing of 3% hydrogen peroxide, control, each processing 500.
High-temperature sterilization processing step: the roundheaded borer of screening is respectively charged into homemade worm box, every lattice one, mutually every
From, then worm box is put into sterilizing frame；It is put into baking oven, to host in sterilizing under the conditions of 60 DEG C, sterilization time is 30 points
Clock；The worm box after sterilization treatment is put between inoculation, room temperature rise again 60 minutes it is spare；
75% alcohol sterilization process steps: the roundheaded borer of screening is put into 75% alcoholic solution, is impregnated 5 seconds, is taken out
Be put into transfer room dry it is spare；
Ultraviolet lamp radiation sterilization processing step: the roundheaded borer of screening is laid in pallet, (distance is put under ultraviolet lamp
It 50cm) irradiates 30 minutes, takes out spare；
3% hydrogen peroxide sterilization process steps: the roundheaded borer of screening is put into 3% hydrogen peroxide solution, is impregnated 5 seconds, is taken
Be put into out transfer room dry it is spare；
Control: sterilization treatment is not done.
(3) polypide bacterium colony detects: every kind of processing takes 10 previous day Niu Jinhang polypide surface pathogen analyte detections at random.By each place
10 previous day oxen of reason are put into 200ml sterile water, and longicorn is taken out in concussion washing 2 minutes.Take 0.5ml above-mentioned with longicorn body surface
The liquid of germ carries out plate culture.It is cultivated 2-3 days for 25 DEG C in incubator, unit of account area clump count.
(4) the rigid larvae of dastarcus helophoroides gently inoculation, tubulature, culture: is inoculated into the day after different disposal with brush
Ox larva body surface, every host connect 15 dastarcus helophoroides larvas, then which is put into the glass dactylethrae to sterilize, every pipe
1, then nozzle is filled in cotton balls.Inoculated roundheaded borer is put into culturing room, is cultivated 45~50 days.
(5) breeding effect counts: after adult all sprouts wings shell out, statistics pollution pipe number, phost line number, adult quantity etc.
Information.
(6) interpretation of result:
Influence of the 1 host's difference sterilization treatment mode of table to breeding index
1. influence of the Different treatments to host insect body surface colony: passing through plate culture and statistical unit area bacterium colony
Number discovery, high-temperature sterilization is inhibited to polypide surface germ with other several sterilization treatment modes, and high temperature is gone out
Bacterium processing mode clump count is minimum, only 2.3/cm2, 87.1% is had dropped compared with control.And the sterilizing of 75% alcohol, ultraviolet light irradiation
Clump count is declined after the processing such as sterilizing, the sterilizing of 3% hydrogen peroxide, but fall is only 35.9-50%.
2. influence of the Different treatments to parasitic rate and pollution rate: statistics Final pollution rate and parasitic rate are, it was also found that height
The parasitic rate of warm sterilization treatment mode improves 6-10% with respect to other processing modes.Final pollution rate has dropped 80.7-
86.9%.
3. influence of the Different treatments to dastarcus helophoroides imago quantum of output: although the more other processing modes of high-temperature sterilization are posted
Raw rate improves only 6-10%, but has in breeding efficiency and significantly improve, and the dastarcus helophoroides imago quantum of output of each host reaches
To 7.8, hence it is evident that be higher than other processing modes, breeding efficiency improves 33.3-47.4%.
Claims (5)
1. a kind of method of dastarcus helophoroides breeding host's sterilization treatment, which comprises the following steps:
(1) select worm: selection body surface does not have wound, free of contamination healthy dastarcus helophoroides breeding host longicorn mature larva；
(2) mounted box: the roundheaded borer screened in step (1) is respectively charged into worm box, and every lattice one are mutually isolated, then by worm
Box is put into sterilizing frame；
(3) it sterilizes: baking oven being arranged to 60 DEG C, is preheating to set temperature, ready sterilizing frame in step (2) is put into baking oven
In, sterilizing frame baffle side avoids hot wind from directly blowing to worm box air outlet, goes out to host in sterilizing under the conditions of 60 DEG C
The bacterium time is 25~35 minutes；
(4) it rises again: the worm box after sterilization treatment in step (3) is put between inoculation, 25 DEG C of room temperatures are risen again 30-60 minutes.
2. according to the method described in claim 1, it is characterized in that sterilization container described in step (2) is that homemade " polypide is gone out
Bacterium box ", material are transparent heat resistant plastic, are made of upper cover (1) and lower cover (2) two parts, 50 1 are dispersed on upper and lower cover
The recess zooecium (3) of × 3.5cm, with after, upper and lower zooecium merges to form 50 mutually isolated " capsule " zooeciums lid.
3. dastarcus helophoroides breeding host longicorn according to claim 1 is Monochamus alternatus Hope.
4. method according to any one of claim 1-3, it is characterised in that sterilization time is 30 minutes.
5. method according to any one of claim 1-3, it is characterised in that the time of rising again is 60 minutes.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201810652834.9A CN108935334B (en) | 2018-06-22 | 2018-06-22 | Method for sterilizing breeding hosts of dastarcus helophoroides |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810652834.9A CN108935334B (en) | 2018-06-22 | 2018-06-22 | Method for sterilizing breeding hosts of dastarcus helophoroides |
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| Publication Number | Publication Date |
|---|---|
| CN108935334A true CN108935334A (en) | 2018-12-07 |
| CN108935334B CN108935334B (en) | 2020-07-21 |
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| CN201810652834.9A Active CN108935334B (en) | 2018-06-22 | 2018-06-22 | Method for sterilizing breeding hosts of dastarcus helophoroides |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112400811A (en) * | 2020-11-25 | 2021-02-26 | 江苏省林业科学研究院 | Method for breeding dastarcus helophoroides on large scale by utilizing thyestillageblerifolius |
| CN112913785A (en) * | 2021-01-29 | 2021-06-08 | 贾自峰 | Method for improving parasitic rate of parasitic tenebrio molitor of dastarcus helophoroides by using longicorn soaking solution |
| CN113180009A (en) * | 2021-04-01 | 2021-07-30 | 江苏省林业科学研究院 | Method for breeding and storing Dastarcus helophoroides adults in simulated habitat |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112400811A (en) * | 2020-11-25 | 2021-02-26 | 江苏省林业科学研究院 | Method for breeding dastarcus helophoroides on large scale by utilizing thyestillageblerifolius |
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| CN112913785B (en) * | 2021-01-29 | 2022-08-02 | 贾自峰 | Method for improving parasitic rate of parasitic tenebrio molitor of dastarcus helophoroides by using longicorn soaking solution |
| CN113180009A (en) * | 2021-04-01 | 2021-07-30 | 江苏省林业科学研究院 | Method for breeding and storing Dastarcus helophoroides adults in simulated habitat |
| CN113180009B (en) * | 2021-04-01 | 2024-01-30 | 江苏省林业科学研究院 | Method for raising and preserving artificial habitat of adult Dastarcus helophoroides |
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