CN106688657A - Method of breeding seedball with longya lily scalesunder LED illuminant - Google Patents
Method of breeding seedball with longya lily scalesunder LED illuminant Download PDFInfo
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- CN106688657A CN106688657A CN201710007415.5A CN201710007415A CN106688657A CN 106688657 A CN106688657 A CN 106688657A CN 201710007415 A CN201710007415 A CN 201710007415A CN 106688657 A CN106688657 A CN 106688657A
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- Prior art keywords
- culture dish
- under
- bulb
- seedball
- led light
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/04—Electric or magnetic or acoustic treatment of plants for promoting growth
- A01G7/045—Electric or magnetic or acoustic treatment of plants for promoting growth with electric lighting
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/08—Immunising seed
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
- A61L2/02—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using physical phenomena
- A61L2/04—Heat
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L9/00—Disinfection, sterilisation or deodorisation of air
- A61L9/015—Disinfection, sterilisation or deodorisation of air using gaseous or vaporous substances, e.g. ozone
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2209/00—Aspects relating to disinfection, sterilisation or deodorisation of air
- A61L2209/20—Method-related aspects
- A61L2209/21—Use of chemical compounds for treating air or the like
- A61L2209/212—Use of ozone, e.g. generated by UV radiation or electrical discharge
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/14—Measures for saving energy, e.g. in green houses
Abstract
Provided is a method of breeding a seedball with longya lily scales under an LED illuminant. The method comprises the steps that three-year-old longya lily commercial seedball scales bred by detoxificated longya lily tissue culture seedling as the seedball is adopted as a material, the material is bred under the independent and continuous illumination of three single LED illuminants with different wave lengths, the longya lily scales are disinfected, the bottom of a culture dish is provided with a non-woven glass transparent petri dish, the culture dish is disinfected under high temperature and high pressure, the growth environment of temperature and humidity controlling is disinfected to culture the material, and nonwoven is wetted quantitatively. According to the technologies, all longya lily scales form spot-shaped small bulbs under the three single LED illuminants with different wave lengths in 30 days, in about 60 days, the propagation coefficient of the longya lily scales under the three single LED illuminants with different wave lengths is 3-8, the small bulb seedball diameter is 0.3 cm-0.6 cm, and the scales of the small bulb seedball diameter are 2 pieces-5 pieces. The method of breeding a seedball with longya lily scales under an LED illuminant has the advantages of being clean, safe, slightly limited by seasons and conducive to factory-like breeding of plants.
Description
Technical field
The present invention relates to the method that Lilium brownii var viridulum scale under a kind of LED light source breeds bulb, platymiscium vegetative propagation technique
Field.
Background technology
Lilium brownii var viridulum (Lilinm brownie var viridulum Baker) is three big famous one of edible, China
Name, special, excellent vegetables, are also collection food medicine dual-purpose food, deep to be welcome by domestic and international market.The main speciality industry in Jiangxi Wanzai County it
One, cultivation history is long.Bulb is Lilium brownii var viridulum Main Cultivation seedling, and current Lilium brownii var viridulum bulb mainly passes through crop field scale skewer
The technologies such as slotting, tissue culture are bred.Crop field scale cuttage reproductive efficiency is low, extensive management, multiple disease the shortcomings of.Tissue culture technology requirement
Higher, input is big, it is many using chemical agent the shortcomings of.
For production provides the method that Lilium brownii var viridulum scale under a kind of LED light source breeds bulb.Relevant LED light source is mainly concentrated
Plant factor metaplasia produces cauline leaf vegetables, really with aspects such as vegetables.Publication number CN103947525 discloses one kind and utilizes LED light source
The method for carrying out romaine lettuce indoor production, the patent provides mixing LED light source cultivation romaine lettuce under being mainly suspension culture environment；It is public
The number of opening CN105284446 discloses a kind of method that utilization LED plant growth light sources promote solanaceous vegetables nursery, patent master
Make solanaceous vegetables nursery under 300nm ~ 470nm waveform LED light sources by oneself.Publication number CN105875228 discloses one kind LED
To the light compensation method of cucumber seedling-raising, the patent mainly carries out light filling to lamp using the combination of red blue light to cucumber seedling；Publication number
CN105210877 discloses a kind of Lilium brownii var viridulum rapid propagation method, and the patent mainly adjusts the raising of tissue culture culture medium condition and lures
Conductance simultaneously shortens the regeneration period；Publication number CN105706708 discloses one kind and breeds Lilium brownii var viridulum kind using Lilium brownii var viridulum scale
The method of ball, the patent is taken shelter from rain using greenhouse, overlay film, summer greenhouse lid sunshade net cooling cultivation mating system.Detoxification fossilia dentis mastodi hundred
Close tissue culture technology Biological Resources Research Institute of Jiangxi Academy of Sciences to have solved, see that Jiang Hongru etc. publishes thesis《Lilium brownii var viridulum stem apex takes off
Malicious fast numerous and bulb Cultivating techniques》Published thesis with Wang Biqin etc.《The influence of Exogenous Substances on Biomass of Test-tube Plantlets of Liliym Brownii Var. Virdulum
Research》.
The content of the invention
The purpose of the present invention is, for Lilium brownii var viridulum scale lining bulb efficiency is low, scale be subject to disease, security it is low,
The problems such as inconvenient plant factor's metaplasia produces bulb, there is provided a kind of method that Lilium brownii var viridulum scale breeds bulb under LED light source.
Realize the technical scheme is that, a kind of method that Lilium brownii var viridulum scale breeds bulb under LED light source, including with
Lower step：
（1）Selection Lilium brownii var viridulum bulb and low-temperature treatment：
It is rotten, imperial without wound, fresh, commercialization that selection has planted 3 years raw nothings with detoxification Lilium brownii var viridulum tissue-cultured seedling as bulb crop field
Tooth lily ball.Bulb paper bag is wrapped up in, then wraps up one layer of plastic foil, is put into 5 DEG C of low-temperature treatments at least 7 days.
（2）Scale is sterilized：
Clean scale is placed in after being processed 10 minutes in 50 DEG C of incubators, with aseptic water washing 5 times, at 70% alcohol-pickled 15 seconds,
Sterilized water is rushed 4 times；Lain against on autoclave sterilization culture dish bottom surface non-woven fabrics rapidly under superclean bench, covered immediately
Culture dish lid.
（3）Three kinds of LED light sources of different wave length of selection carry out illumination：
From three kinds of single LED light sources such as 660nm feux rouges, 450nm blue lights, 300nm ultraviolet lights；Periodicity of illumination is 12h d-1, light
Strong 80 μm of ol m-2∙s-1.The LED light source lamp is fixed in 50 centimeters of upper shelfs of culture dish.
（4）Culture dish bottom surface is lined with the glass transparent culture dish autoclave sterilization with cover of non-woven fabrics：
The glass transparent culture dish with cover that culture dish bottom surface is lined with non-woven fabrics is sterilized 30 minutes for 121 DEG C in autoclave sterilization pot.
（5）Sterilization temperature and humidity control growing environment：
Growing environment temperature is in 23 DEG C ~ 28 DEG C, relative humidity 70%.Every 7 days culture environments ozonization 1 time.
（6）Sterilized water is drawn with pipettor quantitatively moisten glass transparent culture dish bottom surface nonwoven with cover under superclean bench
Cloth
The beneficial effects of the invention are as follows the present invention carries out scale to Lilium brownii var viridulum under LED light source and breeds bulb, the method breeding
Coefficient is high, sterilization control growing environment, can plant factor breed bulb.Solve Lilium brownii var viridulum numerous using conventional method scale
Grow that bulb efficiency is low, scale is subject to the low problem of disease, security.
Specific embodiment
The method that Lilium brownii var viridulum scale breeds bulb under a kind of LED light source of the present embodiment, comprises the following steps：
(1) Lilium brownii var viridulum bulb is chosen and low-temperature treatment
Harvest with detoxification Lilium brownii var viridulum tissue-cultured seedling be 3 years raw commercialization Lilium brownii var viridulum bulbs of bulb field production, selection it is disease-free,
Without wound, scale be long and loose, quality up to the fresh bulb of Lilium brownii var viridulum such as 35 g ~ 50 g, by the fresh bulb exposure 2 of a small amount of soil of band
My god, after bulb paper bag is wrapped up in, then wrap up one layer of plastic foil, low-temperature treatment.
(2) scale sterilization and placement
By the fresh bulb of the Lilium brownii var viridulum of low-temperature treatment certain hour, scale is gently peeled, clean scale and locate in 50 DEG C of incubators
Reason is after 10 minutes, and with aseptic water washing 5 times, at 70% alcohol-pickled 15 seconds, sterilized water was rushed 4 times.It is rapid under superclean bench
Keep flat and be placed on autoclave sterilization culture dish bottom surface non-woven fabrics.Culture dish is positioned over below support as on reflective mirror.Open
Rotating ring border control condition switch, and open LED light source.
(3) LED light source lamp
Independently irradiated from three kinds of single LED light sources such as 660nm feux rouges, 450nm blue lights, 300nm ultraviolet lights, daily continuous illumination
12h∙d-1I.e. from 06:00~18:00.LED light source lamp is fixed in 50 centimeters of upper shelfs of culture dish.
(4) culture dish bottom surface is lined with the glass transparent culture dish autoclave sterilization with cover of non-woven fabrics
Glass transparent 121 DEG C of the culture dish autoclave sterilization pot with cover that culture dish bottom surface is lined with non-woven fabrics is sterilized 30 minutes.Nothing
Spin cloth size in the same size with culture dish basal diameter.And placed before sterilization.
(5) sterilization temperature and humidity control growing environment
23 DEG C ~ 28 DEG C of growing environment temperature, relative humidity 70%.Every 7 days growing environments ozonization 1 time.Temperature is had time
Key section, humidity places psychrometer by humidifier control, and ozone is provided by ozone generator.
(6) draw sterilized water with pipettor and quantitatively moisten culture dish bottom surface with cover non-woven fabrics
Under superclean bench quantitative aseptic water wetting floor non-woven fabrics was drawn every 5 days with pipettor.
Lilium brownii var viridulum scale breeds bulb effect table 1 under three kinds of single LED light sources after 60 days：
From in terms of result, 660nm red-light LEDs light source irradiation bulb is larger preferably, is adapted to comparatively fast breed bulb.450nm blue-ray LED light
Source irradiation breeding coefficient is higher, breeds larger bulb and takes longer for.
Claims (3)
1. a kind of method that Lilium brownii var viridulum scale breeds bulb under LED light source, it is characterised in that methods described includes following step
Suddenly：
（1）Selection Lilium brownii var viridulum bulb and low-temperature treatment：
It is rotten, imperial without wound, fresh, commercialization that selection has planted 3 years raw nothings with detoxification Lilium brownii var viridulum tissue-cultured seedling as bulb crop field
Tooth lily ball；Bulb paper bag is wrapped up in, then wraps up one layer of plastic foil, is put into 5 DEG C of low-temperature treatments at least 7 days；
（2）Scale is sterilized：
Clean scale is placed in after being processed 10 minutes in 50 DEG C of incubators, with aseptic water washing 5 times, at 70% alcohol-pickled 15 seconds,
Sterilized water is rushed 4 times；Lain against on autoclave sterilization culture dish bottom surface non-woven fabrics rapidly under superclean bench, covered immediately
Culture dish lid；
（3）Three kinds of LED light sources of different wave length of selection carry out illumination：
From 660nm feux rouges, 450nm blue lights, three kinds of single LED light sources of 300nm ultraviolet lights；Periodicity of illumination is 12h d-1；Light intensity
80μmol∙m-2∙s-1；
（4）Culture dish bottom surface is lined with the glass transparent culture dish autoclave sterilization with cover of non-woven fabrics：
The glass transparent culture dish with cover that culture dish bottom surface is lined with non-woven fabrics is sterilized 30 minutes for 121 DEG C in autoclave sterilization pot；
（5）Sterilization temperature and humidity control growing environment：
Growing environment temperature is in 23 DEG C ~ 28 DEG C, relative humidity 70%；Every 7 days culture environments ozonization 1 time；
（6）Sterilized water is drawn with pipettor quantitatively moisten glass transparent culture dish bottom surface non-woven fabrics with cover under superclean bench.
2. the method that Lilium brownii var viridulum scale breeds bulb under a kind of LED light source according to claim 1, it is characterised in that
The LED light source lamp is fixed in 50 centimeters of upper shelfs of culture dish.
3. the method that Lilium brownii var viridulum scale breeds bulb under a kind of LED light source according to claim 1, it is characterised in that
The single light of LED light source independently irradiates, daily continuous illumination 12h d-1I.e. from 06:00~18:00.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710007415.5A CN106688657A (en) | 2017-01-05 | 2017-01-05 | Method of breeding seedball with longya lily scalesunder LED illuminant |
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| CN201710007415.5A CN106688657A (en) | 2017-01-05 | 2017-01-05 | Method of breeding seedball with longya lily scalesunder LED illuminant |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107094629A (en) * | 2017-06-11 | 2017-08-29 | 中国科学院植物研究所 | A kind of method for improving hosta tissue culture seedling proliferation rate |
| CN107484589A (en) * | 2017-09-14 | 2017-12-19 | 安徽省万果园农业科技发展有限公司 | A kind of method of lily planting shed environment regulation |
| CN108617325A (en) * | 2018-06-01 | 2018-10-09 | 深圳市昀晨光电有限公司 | A method of promote orchid to grow using LED plant growth lamp |
| CN109757327A (en) * | 2019-03-14 | 2019-05-17 | 三江县丰农农业科技有限公司 | A kind of lily implantation methods |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107094629A (en) * | 2017-06-11 | 2017-08-29 | 中国科学院植物研究所 | A kind of method for improving hosta tissue culture seedling proliferation rate |
| CN107484589A (en) * | 2017-09-14 | 2017-12-19 | 安徽省万果园农业科技发展有限公司 | A kind of method of lily planting shed environment regulation |
| CN108617325A (en) * | 2018-06-01 | 2018-10-09 | 深圳市昀晨光电有限公司 | A method of promote orchid to grow using LED plant growth lamp |
| CN109757327A (en) * | 2019-03-14 | 2019-05-17 | 三江县丰农农业科技有限公司 | A kind of lily implantation methods |
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Address after: 330096 No. 7777 Changdong Avenue, Nanchang City, Jiangxi Province Applicant after: Biological Resources Institute, Jiangxi Academy of Sciences Address before: 330046 No. 382 Shangfang Road, Nanchang City, Jiangxi Province Applicant before: Biological Resources Institute, Jiangxi Academy of Sciences |
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Application publication date: 20170524 |