WO2023103304A1 - Accurate large-scale indoor breeding method for chilo suppressalis using rice seedlings - Google Patents

Abstract

The present invention relates to an accurate large-scale indoor breeding method for Chilo suppressalis using rice seedlings, comprising: cleaning and levigating rice seeds; disinfecting the obtained rice seeds; accelerating germination of the rice seeds to obtain rice sprouts, and then accelerating seedlings to obtain rice seedlings; performing refined larva breeding by using the rice seedlings; forming and collecting pupae; imagoes mating and spawning; and collecting and processing egg masses. According to the present invention, the operation steps of preparation of natural feeds-rice seedlings, larva breeding, pupa formation and collection, mating and spawning of the imagoes, and egg mass collection are accurately quantified, a set of large-scale breeding method is formed, and the breeding time, cost and labor are saved.

Description

A method for precise and large-scale rearing of rice seedlings of Chilo borer indoors technical field

The invention relates to a method for precise and large-scale breeding of rice seedlings indoors by Chilo suppressalis, belonging to the technical field of artificial breeding of insects.

Background technique

As far as the inventor knows, Chilo suppressalis (Walker) belongs to Lepidoptera, Mothidae, is a polyphagous economic pest harmful to rice production, distributed in Asia, the Middle East and southern Europe, especially in the Yangtze River Basin of China And especially in the southern rice area, due to the promotion of hybrid rice and the reform of the farming system, the occurrence of the rice borer has increased year by year, and its larvae feed on the stems and leaves of rice by boring, resulting in a serious reduction in yield. Chilo borer can cause damage to rice in various growth stages. In the stage of rice division, the larvae of the rice stem borer will eat the rice seedlings and cause the rice seedlings to wither; in the booting and heading stages of the rice, the rice stems will be eaten away, so that the rice booting fails, and the rice booting and white ears appear; During the rice filling and milking stages, the larvae of the rice stem borer eat the rice stalks, causing the rice to fail to fill normally, and half-dead ears and insect-damaged plants appear, resulting in more shriveled grains. It can also harm asparagus, sugarcane, sorghum, corn, wheat, millet, arrowroot, broad bean, rapeseed, barnyardgrass, and wild grass. According to statistics, the annual occurrence area of Chilo borer in China is 14 million hectares, and the annual economic loss is 11.5 billion yuan. At present, the control of Chilo borer mainly relies on chemical pesticides to control its outbreak hazards, but Chilo borer has developed resistance to many commonly used insecticides, resulting in an increase in the amount and frequency of application, increasing the cost of control, and causing pressure on the environment. In the process of carrying out control research and formulating control strategies for C. borer, the indoor stable population and large-scale artificial breeding of C. borer can be used to carry out comprehensive control of C. , sex pheromones, transgenic insect-resistant rice, parasitic wasps, etc. provide a stable source of insects at a specific time and age. Therefore, the improvement of the artificial breeding method of the stem borer will promote the control of the stem borer.

Since the 1970s, researches on artificial rearing technology of Chilo suppressalis have been carried out at home and abroad. There are two kinds of artificial feeding techniques for Chilo borer: natural feed feeding and artificial feeding feeding.

The use of rice seedlings or rice stalks as a natural feed is widely used, and on this basis, the methods of raising wild rice stems and water chestnuts have been developed. In the existing natural feed feeding technology, water bamboo, water chestnut and rice are used as natural feed, and the effects of three natural feeds on the growth and fecundity of C. Generational development and reproduction (Meng Fengxia, Wu Kongming, Gao Xiwu. Research on the technology of feeding Chilorrhizae spp. with rice stems, water chestnuts and rice. Insect Knowledge. 2003, 40(5): 469-472), however, limited by feeding conditions, the three species The use of natural feed will be limited by seasons, and it cannot continue to provide sufficient feed for Chilo borer.

In addition to serving as a food source for the stem borer, the natural feed also provides a habitat for the stem borer. Limited by the previous feed antiseptic technology, the host plants used to raise the stem borer are easy to rot, deteriorate or become moldy, and need to be replaced in time during the feeding process. Deteriorated or moldy host plants, rotten, deteriorated or moldy host plants will lead to malnutrition and death of larvae infected with germs, which will reduce the population base of Chilo suppressalis, which is not conducive to large-scale breeding; Problems such as the number and gender imbalance of the stem borer, and the inconsistent growth progress (Shang Zhizhen, Wang Yinshu, Zou Yonghua. Research on feeding methods of the stem borer. Acta Entomology. 1979, 22(2): 164-167; Lu Yurong, Su Jiankun, Ji Chunming , Liu Qin, Zhang Chunmei, Liu Huaia. A Preliminary Study on the Indoor Rearing Method of Rice Chilorrhizae. Anhui Agricultural Sciences. 2003, 31(2): 270-271; Zheng Fushan, Qiang Chengkui, Dong Hongxia, Shen Yuan. Collection and indoor breeding methods. Anhui Agricultural Science. 2009, 37(17): 7837-7838).

The technology of raising Chilo borer with artificial feed started from the development of Ishii feed and Fuye feed. The artificial feed of C. suppressalis has been studied one after another. In 1984, the preliminary research on the practical artificial feed of C. suppressalis solved the problem of the source of C. Generation after generation; the feed is easily corrupted, and the frequency of feed replacement is high. On the basis of this study, the artificial diet of C. melis was further optimized, and the survival rate, pupation rate and eclosion rate of C. barer were all improved, but the growth rate of C. Hu Yang, Zheng Yongli, Cao Guolian, Fu Qiang. Using semi-artificial feed for large-scale and simple feeding of Chilo suppressalis. China Rice Science. 2013, 27(5): 535-538; Liu Huimin, Li Shanhong, Wang Manyu, Zhang Guoan. II Optimization of key factors of artificial feed for Chilo borer and feeding effect of optimized formula. Insect Knowledge. 2008, 45(2): 310-314; Li Bo, Han Lanzhi, Peng Yufa. Artificial feeding technology of Chilo borer. Acta Applied Entomology. , 52(2):498-503).

Artificial feed cannot replace the habitat of natural feed, and the differences between Chilo borer fed with artificial feed and natural feed will lead to deviations in the test results. The raw materials of artificial feed are relatively difficult to obtain, and the cost of feed increases; the process of preparation is cumbersome, requiring feed processing equipment, and the labor cost is high; although the artificial feed has been treated with antiseptic treatment, it needs to be refrigerated at low temperature, which increases the cost of equipment.

After searching, it was found that the invention patent application with application number CN201910423574.2 and application publication number CN110024751A discloses a feeding device and method for Chilo borer, although it uses natural feed to raise Chilo borer, but the device is complicated to make and the operation is cumbersome, etc. The downside. The invention patent application with application number CN201510086108.1 and application publication number CN104604814A discloses a method for efficiently raising Chilo suppressalis. Although it simplifies the raising device, there are still disadvantages such as operational complexity and limited breeding scale.

Application No. CN201210068466.6 and application publication No. CN102578051A for invention patents disclose a method of artificially rearing Chilorrhizae indoors, mainly using small ziplock bags commonly used in laboratories as Chilorrhizae egg beds, not only is the material obtained quite simple, And the concrete operation of collecting egg mass is more simplified, uses the plastic box of 20x15x15 (cm) size simultaneously as container, and the occupied space of whole spawning device is also smaller than when using rice seedlings. Although this technical scheme uses rice seedlings as natural feed, it does not provide the treatment means to overcome the perishable deterioration or mildew of rice seedlings, nor does it provide the treatment means to overcome the inconsistent development progress of Chilo suppressalis. More importantly, its He did not pay attention to how to realize the large-scale feeding of the stem borer, but focused on simplifying the feeding process. It is difficult to realize the large-scale feeding of the stem borer with this technical solution alone, so that a sufficient population of the stem borer cannot be provided.

The invention patent application with application number CN201911073784.X and application publication number CN110604102A discloses a standardized feeding and management method for Chilo suppressalis, including artificial feed preparation, 36-day feeding of larvae, and adult feeding processes. 5 days were used as the cycle of collecting eggs for indoor populations, 4 days were used as the cycle of collecting pupae, and 36 days were used as the larva rearing cycle for a single batch of egg masses inoculated to the pupation stage. Through the standardized management of the operation process of different insect states, a new management system of multi-batch and overlapping cycle feeding has been established. However, this technical solution uses artificial feed instead of natural feed, and cannot take advantage of the advantages of natural feed. At the same time, the operation steps are cumbersome, and even the whole process of feeding from egg mass inoculation to pupation needs to be completed on a sterile operating table. The requirements for the environment and operators are high, which is not conducive to the realization of large-scale breeding.

In a word, feeding C. borer with natural feed has advantages that cannot be achieved by the current artificial feed breeding technology. It is urgent to improve the technology of feeding C. borer with natural feed, simplify the feeding operation steps, save cost and labor, and provide large-scale test. insect.

Contents of the invention

The main purpose of the present invention is: to overcome the problems existing in the prior art, to provide a method for precise and large-scale breeding of rice seedlings indoors, which can accurately quantify the feeding process of the rice stem borer, save time and feeding costs, and solve the problem of the natural host plant rice. Seedling rot and deterioration or moldy problems, reduce the mortality of Chilo borer larvae, increase the pupation rate, ensure the indoor population size of Chilo borer, and can continue to reproduce.

The technical scheme that the present invention solves its technical problem is as follows:

A method for precise and large-scale breeding of rice seedlings indoors by Chilo borer is characterized in that it comprises the following steps:

The first step, cleaning and panning rice seeds; disinfecting the obtained rice seeds;

The disinfection process includes: mixing the sodium hypochlorite solution with tap water at a volume ratio of 1±0.2:100, putting the rice seeds into soak for 24±2 hours, washing the rice seeds with tap water until the sodium hypochlorite is cleaned; soaking the rice seeds in tap water After 24 to 48 hours, wash the rice seeds with double distilled water; the content of active chlorine in the sodium hypochlorite solution is 9 ± 1% by mass;

In the second step, get the rice seeds obtained in the first step, first accelerate germination to obtain rice buds, and then accelerate seedlings to obtain rice seedlings; the gained rice seedlings are located in sterilized canned bottles, and use 50 ± 5g of rice buds on average for each canned bottle; when the rice seedlings grow When it is greater than 3cm and not more than 4cm, the canned bottle is used in the third step;

The third step is placing the aseptically treated egg masses of Chilo stem borer on the rice seedlings in the canned bottle obtained in the second step, with an average of 5-7 egg masses of Chilo suppressalis placed in each canned bottle; the aseptic treatment is fumigation with formaldehyde solution ; The average number of eggs per egg block is 200-300, and the average number of eggs per canned bottle is 1000-2100; Seal the canned bottle with a sterilized black cloth and tie it tightly, and place it at 28°C±1°C and a relative humidity of 70～ In an 80% artificial climate box; add double distilled water to the can bottle once every 2 days, and add water along the bottle wall to moisten the roots of the rice seedlings; it takes 2 to 4 days from placing the egg mass to hatching the first instar larvae; from the first instar larvae It takes 6 to 7 days to develop to the second instar larvae; when the larvae grow to the middle and late stages of the second instar, transfer the larvae in each canned bottle to the rice seedlings in the new canned bottle obtained in the second step, with an average of 900~ 2,000 2nd instar mid-stage larvae; it takes 3 to 4 days to develop from the 2nd instar mid-stage larvae to the 3rd instar mid-stage larvae; when the larvae grow to the end of the 3rd instar larvae, transfer the larvae in each jar to the new canned food obtained in the second step On the rice seedlings in the bottle, an average of 800-1900 end-3rd instar larvae are transferred from each canned bottle; it takes 3-4 days to develop from the end-3rd instar larvae to the end-4th instar larvae. Transfer to the rice seedlings in the new canned bottle obtained in the second step, and transfer 700 to 1800 4th instar larvae per canned bottle on average; it takes 2 to 3 days to develop from the 4th instar late stage larva to the 5th instar late stage larvae, and when the larva grows to 5 In the middle stage of instar larvae, the larvae in each canned bottle are then transferred to the rice seedlings in the new canned bottle obtained in the second step, and an average of 600 to 1700 5th instar mid-stage larvae are transferred out of each canned bottle; After 3 to 4 days, it enters the pupation stage;

The fourth step is to take the sterile roll paper, cut it and fold it into several fan-shaped origami; when the larvae at the end of the 5th instar begin to change into pupae, place the origami on the rice seedlings so that the larvae can move to the gaps or folds of the origami to pupate ;Seal and tie tightly with a sterilized black cloth; after 2 days, take out each origami with a sterilized instrument; first place a sterile paper sheet on the bottom of the petri dish, and then use a sterilized instrument to collect the pupae on the origami to the paper in the petri dish On the sheet, moisten the sheet with double distilled water to maintain the developmental humidity of the pupae;

Step 5: Take a number of petri dishes and place sterile roll paper on the bottom, moisten the sterile roll paper with double distilled water; transfer the pupae collected in step 4 to each petri dish, and place 50±5 per petri dish on average. 1 pupae to prepare for eclosion and mating and oviposition; take some insect cages and place sponges on the bottom, moisten the sponges with double distilled water; in each insect cage, place 2 pots in a diagonal line for spawning Put the rice seedlings with eggs into 2 petri dishes containing pupae; put each insect cage in an artificial climate box at 28°C±1°C and a relative humidity of 70-80%, and the pupae will emerge after 3-4 days, and the adult Mating in pitchers and laying eggs on rice seedlings in jars;

Step 6: After 6 to 7 days of adult emergence, take out the rice seedlings from the insect cage, cut off the leaves of the rice seedlings carrying the egg mass with a sterilized instrument, and put them into a petri dish with moist filter paper on the bottom. 100-150 egg masses were obtained from the insect cage; the petri dish was fumigated with formaldehyde solution to aseptically treat the egg masses; the obtained egg masses were used for the next cycle of cultivation and rearing.

In the method, the rice seeds are disinfected, which can avoid subsequent rot and deterioration of rice seedlings or mold, thereby solving the problem of perishable natural feed, and avoiding large-scale death of Chilo borer larvae affected by rice seedling rot and deterioration or mold; Strictly limit the amount of rice buds per canned bottle, which is conducive to the accurate and quantitative feeding process, and lays the foundation for the subsequent large-scale feeding of Chilo suppressalis; the time node and rice seedling transfer operation between egg mass hatching to the end of the fifth instar larvae are precisely limited, which not only realizes feeding The precise quantification of the process can also ensure a high survival rate of Chilo suppressalis larvae through such fine operations, so as to form a large-scale breeding scale; in the process of pupation and harvesting, the simple carrier of fan-shaped origami is The pupae can be collected smoothly, making the feeding process easier; during the mating and oviposition process of adults after eclosion, rice seedlings are used as the natural substrate for adult oviposition to increase the oviposition rate and facilitate subsequent large-scale breeding. This feeding method can be carried out repeatedly, so as to continuously provide multiple batches of large-scale development progress (worm state, age, individual size, health status, sensitivity, etc.) consistent insect source for the control research of Chilo borer , to ensure the accuracy of repeated experiments and shorten the research cycle; the process is simple, and only one person can complete all the breeding operations.

The further perfect technical scheme of the present invention is as follows:

Preferably, the jar containing larvae in the third step, the petri dish containing pupae in the fourth step, or the egg masses obtained in the sixth step are placed in an environment of 4°C±2°C for a preset time to control their developmental progress.

After adopting this optimal scheme, the growth and development of the egg stage, larval stage and pupal stage of Chilo borer can be further controlled by adjusting the temperature, which can not only make its development progress more consistent, but also meet the requirements of the corresponding control research projects on the development stage of insect sources. time window requirements.

Preferably, in the first step, the specific process of cleaning and panning rice seeds is:

Put the rice seeds into the pot, pour tap water into the pot and submerge the rice seeds, and let it stand for a few minutes, so that the rice husks or dry rice seeds float on the water surface, while the full rice seeds sink to the bottom of the pot; the rice husks or dry rice seeds Pour the seeds together with the water into the drain basin, leaving only the full rice seeds at the bottom of the basin; repeat the above process until the full rice seeds sinking into the bottom of the basin are cleaned until the water becomes clear; the average weight of rice seeds in each pot is greater than 1000g , the final average weight of full rice seeds per pot is 93 ± 3% of the rice seeds put in.

After adopting the above optimal scheme, the specific technical details of the first step can be further optimized to make it more simplified; at the same time, a large amount of rice seed is used, which is beneficial to continuously provide rice seedlings as natural feed in the future.

Preferably, in the second step, the specific process of accelerating germination to obtain rice buds is:

Take the rice seeds obtained in the first step, drain the rice seeds and put them into a pot, then seal the pot with a clean black plastic bag and tie the bag tightly, and place it at 28°C±1°C and a relative humidity of 70～ In an 80% artificial climate box, to accelerate the dew and break the chest of the seeds; turn the rice seeds once at 24±2 hours to make the temperature of the rice seeds uniform and ensure that the germination is neat; at 48±2 hours, the rice seeds have fully germinated, which is the rice buds.

Preferably, in the second step, the specific process of accelerating seedlings to obtain rice seedlings is:

Wash the rice buds with double distilled water, and drain the water; place a sterile paper sheet on the bottom of the clean canning bottle as a carrier for the rice seeds to take root; use double distilled water to moisten the paper sheet, and sow the rice buds in the canning bottle In the process, seal with plastic wrap, prick holes with needles to ensure unobstructed oxygen, and place in an artificial climate box at 28°C±1°C and relative humidity of 70-80% to accelerate the emergence of rice seeds; Rinse the rice seedlings with water, and then pour the double distilled water to maintain the moisture required for the growth of the rice seedlings.

After adopting the above optimal scheme, the specific technical details of the second step can be further optimized to make it more simplified, and a large number of rice seedlings with consistent growth progress can be cultivated in each batch, so as to provide sufficient natural feed for subsequent feeding.

Preferably, in the third step, use tweezers sterilized with medical alcohol, burn them with an alcohol lamp and cool them down naturally for later use; The rice seedlings are placed on the first clean paper, and the larvae are picked out from these rice seedlings and placed on the second clean paper, and finally the larvae on the second clean paper are poured directly into the new canned rice seedlings At this time, if any larva escapes from the clean paper, use tweezers to pick it on the rice seedlings in a new can; after that, seal the can with a sterilized black cloth and tie it tightly, and continue to store it at 28°C±1 ℃, relative humidity 70-80% in an artificial climate box; in the third step, the larvae are reared in an artificial climate box with a consistent environment throughout.

After adopting the above optimal scheme, the specific technical details of the third step can be further optimized, which can better ensure that the larvae will not die in large areas due to the replacement of seedlings; keeping the feeding environment consistent can ensure that the larvae enter each developmental stage neatly.

Preferably, in the fourth step, when taking out each origami, put the origami into a sterilized glass jar, and then perform subsequent operations; the sterilized instruments are medical tweezers sterilized by medical alcohol; the caliber of the petri dish used is 90mm; In the fifth step, the caliber of the petri dish used is 90mm; use 100±10mL double distilled water to moisten the sponge.

After adopting the above preferred scheme, the specific technical details of the fourth and fifth steps can be further optimized to achieve better technical effects.

Preferably, in the fifth step, the process of cultivating rice seedlings for laying eggs is: take the rice buds obtained by accelerating germination in the second step, sow them on the soil surface of the preset pot and cover with a thin layer of soil, and use a clean Cover the pot with a black plastic bag, and place it in an artificial climate chamber at 28°C ± 1°C and a relative humidity of 70-80% to cultivate until the height of the rice seedling reaches 10 cm, which is the rice seedling used for laying eggs; in the fifth step, A petri dish with a caliber of 60mm is also placed in the insect cage, and the petri dish is filled with sterilized cotton balls dipped in honey water with a volume ratio of 10±1%; the cotton balls are replaced every two days.

After adopting the above preferred scheme, the additional specific technical details in the fifth step can be further optimized, wherein the rice seedling cultivation process for laying eggs is precisely defined, which can better provide the spawning environment; honey water can be used for eclosion adults Supplement nutrition to promote its oviposition, and use sterile cotton balls to prevent adults from falling into honey water and dying.

Preferably, in the sixth step, the specific process of the fumigation is: place the petri dish containing the egg mass in a drying cylinder filled with formaldehyde solution at the bottom and fumigate it for 15 ± 1min, and then take it out immediately; the sterilized instruments are sterilized scissors; the caliber of the petri dish used is 90 mm; the concentration of the formaldehyde solution is 35 ± 1% by volume.

After adopting the above preferred scheme, the specific technical details of the sixth step can be further optimized, wherein the fumigation process can kill the parasites on the surface of the egg mass, which is beneficial to improve the survival rate of the larvae in the subsequent rearing process.

Preferably, the rice seeds are Japonica rice seeds; the can bottle has a diameter of 6cm and a height of 10cm; the size of the insect cage is: 45cm×45cm×55cm; the number of can bottles used in the third step is greater than 20 bottles.

The present invention accurately quantifies the operation steps of preparing natural feed rice seedlings, raising larvae, pupating and harvesting pupae, mating and laying eggs of adults, and collecting egg masses, and forms a set of large-scale feeding methods, saving feeding time, cost and labor.

Compared with the prior art, the main advantages of the present invention are: (1) take corresponding disinfection and antibacterial measures in the whole process of egg-larvae-pupa-adult-egg, comprehensively control the infection of pathogenic bacteria, and ensure the development and development of generations. population size. (2) The preparation of natural feed includes cleaning, disinfection, germination and sowing of rice seeds, which effectively controls the rot and deterioration of rice seedlings or mildew, provides sufficient nutrition for larvae, optimizes the habitat environment of larvae, and reduces the number of natural feed replacements , improve the survival rate of larvae. (3) Provide a new set of pupation and pupa collection methods, so that advanced larvae can drill and cocoon and pupate at the folding place of origami, providing a safe, suitable humidity, and airtight pupation place, which can increase the number of pupations and increase the weight of pupae , can be preserved and transported with origami as a unit, finding pupae is simple and convenient, suitable for large-scale subculture. (4) The egg-laying environment of the adult Chilo suppressalis is optimized, and an insect cage suitable for the egg-laying of the Chilo borer is adopted, so that the adults can mate and lay eggs in a sterile constant temperature and humidity environment, and the amount of eggs laid is improved; natural feed is adopted Rice seedlings provide an oviposition substrate that restores the natural way of life of the stem borer, increasing the oviposition rate. (5) The biological characteristics of the rice stem borer raised according to the method of the present invention are close to those of the rice stem borer in nature, and the research results of related research have little deviation.

Description of drawings

Fig. 1 is a schematic diagram of preparation of natural feed for Chilo suppressalis in Example 1 of the present invention.

Fig. 2 is a schematic diagram of rearing larvae of rice stem borer japonica seedlings in Example 2 of the present invention.

Fig. 3 is a schematic diagram of harvesting pupae of Chilo suppressalis in Example 3 of the present invention.

Fig. 4 is a schematic diagram of mating and oviposition of adult Chilo suppressalis in Example 4 of the present invention.

Fig. 5 is a schematic diagram of the collection and storage of egg masses of Chilo suppressalis in Example 5 of the present invention.

Detailed ways

The present invention will be further described in detail below in conjunction with examples. However, the invention is not limited to the examples given. The reagents involved in the present invention are all commercially available.

Embodiment 1, preparation of natural feed for Chilo borer

In this example, the japonica rice variety Nanjing 46 was used as the natural feed for Chilo suppressalis. This variety is the main variety in Jiangsu Province and is widely planted in various cities and counties in Jiangsu Province. In the present invention, different rice varieties can be selected as the natural feed, and local rice varieties can be selected to facilitate local material acquisition, and can also conform to the actual living environment of the local rice stem borer.

(1) Weigh 1500g of Nanjing japonica 46 seeds with a balance. The weight of the japonica rice seeds can meet the needs of large-scale reproduction of Chilo suppressalis, and can provide host food and habitat for subsequent rearing.

Put the weighed Nanjing 46 seeds into a 10L plastic basin, pour 5L of tap water into the plastic basin with a measuring cup, vigorously stir the Japonica rice seeds in the tap water, let it stand for a few minutes, and pour the remaining rice husks or dry rice seeds into the plastic basin. In the draining basin, leave the plump rice seeds at the bottom of the basin. Then pour 5L of tap water into a plastic basin with a measuring cup, stir the japonica rice seeds vigorously, and remove the remaining rice husks or dry rice seeds. This elutriation step is repeated 5 times in total, and rice husks or dry rice seeds are removed.

The japonica rice seeds that sink to the bottom of the plastic basin weigh about 1400g. Use a measuring cup to pour 3L of tap water into the plastic basin to soak the japonica rice seeds for subsequent disinfection.

(2) According to the ratio of sodium hypochlorite solution and tap water 1:100, 0.03L of sodium hypochlorite solution (containing active chlorine 9% by weight, the same below) is poured into 3L of tap water, fully stirs and mixes, pours the sodium hypochlorite that soaks Japonica rice seed after 24h Mix it with tap water, and wash the rice seeds fully with tap water until the irritating smell of sodium hypochlorite is no longer smelled.

Pour 3L of tap water into a plastic basin with a measuring cup and continue to soak the japonica rice seeds. The standard for the seeds to be fully soaked is that the chaff is transparent and the white belly of the rice grains is visible. The rice grains are easy to break without making a sound after peeling off the glumes. Get plenty of moisture. After 24h, pour the tap water soaking the japonica seeds.

Pour 2L of double distilled water into a measuring cup to wash the soaked japonica rice seeds. After fully cleaning, pour the double distilled water. Repeat the cleaning process 3 times. This operation step can wash away the trace chemical components in tap water to prevent the seeds from not sprouting Chilo borer poisoning.

(3) After draining the rice seeds, wrap the plastic basin with a black plastic bag and tie the mouth of the plastic bag tightly. The black plastic bag has a good heat preservation effect and is conducive to the germination of the japonica rice seeds. Place it in a model RGC-1000C artificial climate chamber (Hefei Youke Instrument Equipment Co., Ltd.), set the temperature at 28°C, relative humidity at 80%, light:dark=16h:8h. During the germination process, it is necessary to keep the temperature not lower than 25°C to prevent high-temperature burning of seeds, and seeds above 40°C will lose their ability to germinate. Turn the japonica rice seeds once every 24 hours. When turning, the residual moisture at the bottom of the plastic pot can be fully mixed with the seeds, and the ventilation and oxygenation can be increased to accelerate the vigorous growth of the seed buds.

(4) After 48 hours, after the japonica rice seeds have germinated as a whole, pour 2L of double distilled water into the measuring cup and gently wash the germinated rice seeds. Repeat this process twice. Do not break the rice buds during cleaning, and fully drain the water. Commercially available aseptic roll paper was cut into paper sheets with a length of 13.9 cm and a width of 10.2 cm, which were used as substrates for the growth of japonica rice seeds. Put the paper sheet into the bottom of a 500mL can bottle with a diameter of 6cm and a height of 10cm, pour 10mL of double distilled water into the measuring cylinder to moisten it, then weigh 50g of soaked japonica rice buds, and sow them in the can bottle. Cut ordinary plastic wrap to the size of the bottle mouth of the can, then seal the bottle mouth with it, and use 0 ^# insect needles to pierce 10-15 holes in the plastic wrap to ensure unobstructed oxygen. 1400g japonica rice buds can be planted with 28 bottles of rice seedlings, placed in a model RGC-1000C artificial climate box (Hefei Youke Instrument Equipment Co., Ltd.), set temperature 28 ° C, relative humidity 80%, light:dark=16h:8h. In order to ensure the germination of japonica rice buds, it is necessary to keep the temperature not lower than 25°C. After 72 hours, the rice buds grow to 1-2 cm. Pour 5 mL of double distilled water along the inner wall of the bottle to prevent the rice seedlings from drying out. During the emergence process, rinse the rice seedlings with 100 mL of double distilled water every 48 hours, and then dump the double distilled water. This step can ensure the water required for the growth of rice seedlings.

The above process is shown in Figure 1.

Embodiment 2. Chilo borer larva rearing

(1) Rearing of first instar larvae

When the japonica rice seedlings in the canned bottle grow to be greater than 3cm, put the aseptic egg masses of Chilo suppressalis of the same size in the rice seedlings of the canned bottle, the number of placed egg masses is 5, and the placed egg masses are egg masses that are about to hatch into larvae The criterion for judging is that the egg masses are black and have been placed in the artificial climate box for 2 days. The number of canned bottles for placing egg masses is 28 bottles. The size of the egg block used in this embodiment is length 15mm and width 2mm, the number of eggs in the egg block is 200-300, the number of eggs in the canned bottle is 1000-1500, and the total number of eggs in one generation is 28000-42000. The canning bottle is sealed with a black cloth and tied tightly with a rubber band (as shown in Figure 2). Place it in a model RGC-1000C artificial climate chamber (Hefei Youke Instrument Equipment Co., Ltd.), set the temperature at 28°C, relative humidity at 80%, light:dark=16h:8h. The first instar larvae hatched after 2 days from the egg mass, and 10 mL of double-distilled water was added along the wall of the can bottle every 48 hours during the larval growth stage to moisten the roots of the rice seedlings. In the 1st instar larva rearing stage, prepare the japonica rice seedlings required in the larval rearing according to Example 1, keeping the same number of bottles.

(2) Second instar larva rearing

It takes 7 days for the 1st instar larvae of Chilo borer to grow to the 2nd instar larvae. At this stage, there is no need to replace the japonica rice seedlings, only to ensure sufficient water for the rice seedlings to grow. In the middle and late stages of the second instar, as the insect body increases, the food intake begins to increase, and the japonica rice seedlings need to be replaced. First, remove the tweezers from the 75% sterilizing alcohol, light the alcohol lamp, burn the tweezers above the flame of the alcohol lamp for the purpose of sterilizing the tweezers, and then place them on sterile roll paper to cool naturally. Spread 2 sheets of A4 copy paper on the test bench, one is used to temporarily place the japonica rice seedlings clipped from the can bottle, the 2nd instar larvae live in the japonica rice seedlings, and the other is temporarily placed from the japonica rice seedlings Pick out the 2nd instar larvae. Pour the 2nd instar larvae on A4 copy paper directly into the jars containing fresh japonica rice seedlings. The escaped 2nd instar larvae are picked into the jars with tweezers, and 900-1400 2nd instar larvae can be transferred out of each jar. After the transfer is completed, seal it with a black cloth, tie it tightly with a rubber band, and place it in a model RGC-1000C artificial climate box (Hefei Youke Instrument Equipment Co., Ltd.), set the temperature at 28°C, relative humidity at 80%, light: dark=16h : 8h.

Put the sealed black cloth used by the 1st instar larvae in a plastic basin, and pour it into a 2L tap water plastic basin with a measuring cup. According to the sodium hypochlorite solution: water = 1:100, the required amount of sodium hypochlorite solution is 20mL, and pour it into the plastic basin. Mix with tap water. After soaking for 24 hours, fully rinse under tap water until the pungent smell of sodium hypochlorite can no longer be smelled. Drop a few drops of cold water degreasing detergent in a plastic basin, soak and clean the sealed black cloth under tap water, rinse it, wring it dry, and put it in a model WGL-125B electric blast drying oven (Tianjin Tester) Instrument Co., Ltd.) and the temperature was set at 50 °C. Use a measuring cup to pour 500 mL of tap water in a canned bottle used by the 1st instar larvae to raise. According to the sodium hypochlorite solution: water=1:100, the required amount of sodium hypochlorite solution is 5 mL, pour it into the canned bottle and mix with tap water. After soaking for 24 hours, fully rinse under tap water until the pungent smell of sodium hypochlorite can no longer be smelled. Add 1 to 2 drops of cold water degreasing detergent to the can, wash the can under tap water, and rinse off the foam. Place the canned bottle in a digital display blast drying oven of model GZX-9140ME and bake at a high temperature. The temperature is set at 200°C, and the baking time is 6 hours. After the baking is completed, it is naturally cooled. Then place the sealing black cloth and the canning jar in the artificial climate box, turn on the ultraviolet lamp, sterilize under the ultraviolet light for 30 minutes, and after the wind blows for 10 minutes, take out the sealing black cloth and the canning jar for later use.

(3) Third instar larva rearing

It takes 4 days for the 2nd instar mid-stage larvae of Chilo borer to develop into the 3rd instar mid-stage larvae, and the japonica rice seedling needs to be replaced once at the 3rd instar larvae stage. Take out the tweezers from the 75% disinfectant alcohol, put them on the flame of the alcohol lamp for burning, and give the tweezers Sterilize and cool naturally on sterile roll paper. Lay 2 sheets of A4 copy paper on the test bench, one is used to temporarily place the japonica rice seedlings clipped from the can bottle, the 3rd instar larvae live in the japonica rice seedlings, and the other is temporarily placed from the japonica rice seedlings Pick out the 3rd instar larvae. Pour the 3rd instar larvae on the A4 copy paper directly into the jars containing fresh japonica rice seedlings, pick the escaped 3rd instar larvae into the jars with tweezers, 800-1300 3rd instar larvae can be transferred out of each jar. After the transfer is completed, seal it with a black cloth, tie it tightly with a rubber band, and place it in a model RGC-1000C artificial climate box (Hefei Youke Instrument Equipment Co., Ltd.), set the temperature at 28°C, relative humidity at 80%, light: dark=16h : 8h. Similarly, according to the operating steps of (2) in Example 2, the black cloth for sealing and the canning bottle are sterilized for subsequent use. According to the operation steps in Example 1, the japonica rice seedlings required for larval rearing were prepared, and the number of bottles was kept the same.

(4) Fourth instar larva rearing

The end of the 3rd instar enters the "gluttony period" until the end of the 4th instar larvae, and the rice seedlings are replaced once between the end of the 3rd instar larvae and the end of the 4th instar larvae. Take the tweezers out of the 75% sterilizing alcohol, burn them over the flame of an alcohol lamp, sterilize the tweezers, and place them on sterile roll paper to cool naturally. Spread 2 sheets of A4 copy paper on the test bench, one is used to temporarily place the japonica rice seedlings clipped from the can bottle, the 4th instar larvae live in the japonica rice seedlings, and the other is temporarily placed from the japonica rice seedlings Pick out the 4th instar larvae. Pour the 4th instar larvae on A4 copy paper directly into the jars containing fresh japonica rice seedlings. The escaped 4th instar larvae are picked into the jars with tweezers, and 700-1200 4th instar larvae can be transferred out of each jar. After the transfer is completed, seal it with a black cloth, tie it tightly with a rubber band, and place it in a model RGC-1000C artificial climate box (Hefei Youke Instrument Equipment Co., Ltd.), set the temperature at 28°C, relative humidity at 80%, light: dark=16h : 8h. Similarly, according to the operating steps of (2) in Example 2, the black cloth for sealing and the canning bottle are sterilized for subsequent use. According to the operation steps in Example 1, the japonica rice seedlings required for larval rearing were prepared, and the number of bottles was kept the same.

(5) Fifth instar larva rearing

After the larvae at the end of the 4th instar enter the 5th instar larvae, their food intake decreases and they begin to cocoon and pupate. The rice seedlings were replaced once in the middle of the 5th instar, and the tweezers were taken out of the 75% disinfectant alcohol, burned above the flame of an alcohol lamp, sterilized and sterilized, and placed on sterile roll paper to cool naturally. Spread 2 sheets of A4 copy paper on the test bench, one is used to temporarily place the japonica rice seedlings clipped from the can bottle, the 5th instar mid-stage larvae inhabit among the japonica rice seedlings, and the other temporarily places the japonica rice seedlings from the 5th instar mid-stage larvae picked out from Pour the mid-fifth instar larvae on A4 copy paper directly into the jars containing fresh japonica rice seedlings, pick the escaped mid-fifth instar larvae into the jars with tweezers, and transfer 600 to 1100 mid-fifth instar larvae from each jar larva. After the transfer, seal it with a black cloth and tie it tightly with a rubber band. 6 sheets of paper (length 13.9 cm and width 10.2 cm) cut from aseptic roll paper were folded into fan-shaped origami, placed on the japonica rice seedlings, sealed with black cloth, and tied tightly with rubber bands. Place it in a model RGC-1000C artificial climate chamber (Hefei Youke Instrument Equipment Co., Ltd.), set the temperature at 28°C, relative humidity at 80%, light:dark=16h:8h. The larvae reared in this embodiment were placed in an environment with constant temperature and humidity and consistent light throughout the whole process, and the larvae at the end of the fifth instar stage neatly entered the pupation stage.

Embodiment 3. Chilo suppressalis pupates and collects pupae

(1) Chilo borer pupate (as shown in Figure 3)

The 5th instar larvae of Chilo borer enter the pupation stage after 3 days, and the larvae will crawl to the gaps of the origami to pupate, and the larvae at the end of the 5th instar hardly eat, and only need to wait for the larvae to pupate at this stage. In the glass cylinder (diameter 18cm and height 9cm) where the origami is to be placed, pour 2L of tap water into a measuring cup, according to the sodium hypochlorite solution: water = 1:100, the required amount of sodium hypochlorite solution is 20mL, pour it into the glass cylinder and mix with tap water . After soaking for 24 hours, fully rinse under tap water until the pungent smell of sodium hypochlorite can no longer be smelled. Drop a few drops of cold water degreasing detergent into the glass jar, wash the glass jar under tap water, and rinse off the foam. Place the glass cylinder in a digital display blast drying oven of model GZX-9140 ME and bake at high temperature. The temperature is set at 200°C, and the baking time is 6 hours. After the baking is completed, it is naturally cooled. Then the glass jar was sterilized by ultraviolet light for 30 minutes under the ultraviolet lamp in the artificial climate box, and after the wind was blown for 10 minutes, the glass jar was taken out for standby. Put a 90mm glass petri dish in a plastic basin, pour tap water and drop a few drops of cold water degreasing detergent, stir the tap water thoroughly, clean the glass petri dish with a brush, dry the glass petri dish naturally after cleaning thoroughly, and wipe it with newspaper Pack them in groups of 10, put them in a D-1 automatic steam sterilizer (Beijing Faen Technology and Trade Co., Ltd.) for sterilization, set the temperature at 120°C, sterilize for 20 minutes, and place them in a model WGL-125B electric heater after sterilization Dry in a blast drying oven (Tianjin Test Instrument Co., Ltd.), and set the temperature to 50°C. After drying, take it out for use. This operation can reuse the culture dish and reduce the cost.

(2) Chilo borer harvests pupae (as shown in Figure 3)

Take the tweezers out of the 75% sterilizing alcohol, burn them over the flame of an alcohol lamp, sterilize the tweezers, and place them on sterile roll paper to cool naturally. After 2 days, the origami containing the pupation was taken out with tweezers, and the origami was placed in the above-mentioned sterilized glass jar. At the same time, 6 pieces of paper (length 13.9 cm and width 10.2 cm) cut from sterile roll paper were folded into fan-shaped origami, placed on the top of the japonica rice seedlings, sealed with a sterilized black cloth, and tied tightly with a rubber band to allow the larvae to Continue to pupate. Place it in a model RGC-1000C artificial climate chamber (Hefei Youke Instrument Equipment Co., Ltd.), set the temperature at 28°C, relative humidity at 80%, light:dark=16h:8h. After 2 days, put a piece of A4 copy paper on the test bench, clamp the origami to the A4 copy paper with tweezers, pull the origami apart with 2 tweezers, and clamp the pupae in the origami into a 90mm glass petri dish, and place the chrysalis on the bottom of the petri dish. Pave the bedding with sterile roll paper, and moisten the sterile roll paper with double distilled water to keep the development humidity of the pupae.

Example 4. Adults of Chilo suppressalis mating and laying eggs

(1) Preparation of insect cages

Rinse the insect cage (45cm×45cm×55cm) that will be used for mating and laying eggs of Chilo suppressalis first with tap water. Then use a measuring cup to pour 5L of tap water into a plastic basin, according to sodium hypochlorite solution: water=1:100, the required amount of sodium hypochlorite solution is 50mL, pour it into a plastic basin and mix with tap water to prepare disinfectant. Use an ordinary brush dipped in this proportion of disinfectant to scrub and disinfect the insect cages that are going to be used for mating and laying eggs of Chilo borer. After washing with this disinfectant, rinse it under tap water until you can no longer smell the pungent smell of sodium hypochlorite. Place it in a room temperature environment to dry for later use.

(2) Preparation of egg-laying japonica rice seedlings

According to the operation steps in Example 1., the rice buds of the required egg-laying japonica rice seedlings were prepared. Sow 50 g of sterilized rice buds in plastic pots (caliber 17.5 cm and height 15.5 cm), sprinkle them on the soil surface of the pots, cover with a thin layer of soil, place them in an artificial climate chamber, and control the temperature to 28 ° C with air conditioning. The cycle is 16 hours of light, 8 hours of darkness (controlled by a timer), and the relative humidity is about 80%. Cover the plastic pot with a black plastic bag to keep it warm. After 7 days, the rice buds emerged in the plastic pot, as the seedlings that lay eggs, and the plant height of the seedlings that lay eggs is 10cm.

(3) Adults mate and lay eggs (as shown in Figure 4)

The collected pupae were transferred to a 90 mm glass petri dish, the bottom of the petri dish was paved with sterile roll paper, the sterile roll paper was moistened with double distilled water, and 50 pupae were placed in each petri dish for mating and oviposition. First, place the sterilized insect cages in a model RGC-1000C artificial climate box (Hefei Youke Instrument Equipment Co., Ltd.), set the temperature at 28°C, relative humidity at 80%, light:dark=16h:8h. Place a suitable-sized absorbent sponge on the bottom of the insect cage, moisten the absorbent sponge with 100 mL of double distilled water, place two pots of ovipositing rice seedlings in the insect cage in a diagonal arrangement, and then Place the pupae of 2 boxes of Petri dishes in the insect cage.

Use a 1000uL pipette gun to draw 10mL of pure honey and transfer it to a disposable cup, pour 90mL of double distilled water into it with a measuring cylinder, stir it fully with an iron medicine spoon, and prepare 10% honey water. Pour 20mL of 10% honey water into a 60mm petri dish, and use a sterilized cotton ball to dip into the honey water to provide feeding carriers for the adults and prevent the adults from falling into the honey water and dying. Put a petri dish with honey water into the insect cage to supplement nutrition for the adult worms that have emerged to promote their oviposition. Change the honey water in the petri dish once every 2 days to ensure the freshness of the honey water and prevent mold and corruption. Placed in an artificial climate box at a temperature of 28°C, pupae emerge after 4 days, and the emerged adults mate and lay eggs in insect cages. The plant height of egg-laying seedlings grows to 20cm.

Example 5. Collection and preservation of egg masses of Chilo suppressalis

After 7 days of adult emergence, the pots of japonica rice seedlings were removed from the artificial climate chamber. If the adults of Chilo suppressalis were still attached to the rice seedlings, the adults could be removed from the rice seedlings. At this stage, the vitality of the adults was weakened and inactive. Emerged adults lay their eggs on the surface of rice leaves, and the eggs gather together to form egg masses. Soak the scissors in 75% alcohol, take them out and wipe them with sterile roll paper for disinfection. First spread 1 sheet of A4 copy paper on the test bench, with the scissors that have been sterilized, the rice plant with ovum mass is cut off from the root, and temporarily placed on the A4 copy paper. Prepare to place a piece of sterile rolling paper on the bottom of a 90mm petri dish, and moisten the paper with double distilled water. Cut off the paddy rice leaves carrying the ovum mass with sterilized scissors, and put them into a 90mm Petri dish with a wet paper sheet at the bottom. 2 pots of egg-laying seedlings can cut out 100 japonica rice leaves with egg masses, and 100-150 egg masses.

Take 35mL of formaldehyde with a 5mL pipette gun and transfer it to a disposable cup, pour 65mL of double distilled water into it with a graduated cylinder, and stir thoroughly to prepare a 35% formaldehyde solution. Place the petri dish with the ovum on the glass desiccator filled with formaldehyde solution at the bottom and fumigate for 15min, and take it out immediately after the end. The purpose is to sterilize the ovum by fumigation and kill the parasites on the surface of the egg (as shown in Figure 5).

Afterwards, place the egg mass in an artificial climate box at 28°C for 0 to 2 days for the next cycle of cultivation and feeding; or, put the egg mass in a refrigerator at 4°C for low-temperature refrigeration to keep the development progress of the egg consistent and control the growth of the egg mass. incubation time.

In addition to the above-mentioned embodiments, the present invention can also have other implementations. All technical solutions formed by equivalent replacement or equivalent transformation fall within the scope of protection required by the present invention.

Claims (10)

1. A method for precise and large-scale breeding of rice seedlings indoors by Chilo borer is characterized in that it comprises the following steps:

   The first step, cleaning and panning rice seeds; disinfecting the obtained rice seeds;

   The disinfection process includes: mixing the sodium hypochlorite solution with tap water at a volume ratio of 1±0.2:100, putting the rice seeds into soak for 24±2 hours, washing the rice seeds with tap water until the sodium hypochlorite is cleaned; soaking the rice seeds in tap water After 24 to 48 hours, wash the rice seeds with double distilled water; the content of active chlorine in the sodium hypochlorite solution is 9 ± 1% by mass;

   In the second step, get the rice seeds obtained in the first step, first accelerate germination to obtain rice buds, and then accelerate seedlings to obtain rice seedlings; the gained rice seedlings are located in sterilized canned bottles, and use 50 ± 5g of rice buds on average for each canned bottle; when the rice seedlings grow When it is greater than 3cm and not more than 4cm, the canned bottle is used in the third step;

   The third step is placing the aseptically treated egg masses of Chilo stem borer on the rice seedlings in the canned bottle obtained in the second step, with an average of 5-7 egg masses of Chilo suppressalis placed in each canned bottle; the aseptic treatment is fumigation with formaldehyde solution ; The average number of eggs per egg block is 200-300, and the average number of eggs per canned bottle is 1000-2100; Seal the canned bottle with a sterilized black cloth and tie it tightly, and place it at 28°C±1°C and a relative humidity of 70～ In an 80% artificial climate box; add double distilled water to the can bottle once every 2 days, and add water along the bottle wall to moisten the roots of the rice seedlings; it takes 2 to 4 days from placing the egg mass to hatching the first instar larvae; from the first instar larvae It takes 6 to 7 days to develop to the second instar larvae; when the larvae grow to the middle and late stages of the second instar, transfer the larvae in each canned bottle to the rice seedlings in the new canned bottle obtained in the second step, with an average of 900~ 2,000 2nd instar mid-stage larvae; it takes 3 to 4 days to develop from the 2nd instar mid-stage larvae to the 3rd instar mid-stage larvae; when the larvae grow to the end of the 3rd instar larvae, transfer the larvae in each jar to a new canned food obtained in the second step On the rice seedlings in the bottle, an average of 800-1900 end-3rd instar larvae were transferred from each canned bottle; it took 3-4 days to develop from the end-3rd instar larvae to the end-4th instar larvae. Transfer to the rice seedlings in the new canned bottle obtained in the second step, and transfer 700 to 1800 4th instar larvae per canned bottle on average; it takes 2 to 3 days to develop from the 4th instar late stage larva to the 5th instar late stage larvae, and when the larva grows to 5 In the middle stage of instar larvae, the larvae in each canned bottle are then transferred to the rice seedlings in the new canned bottle obtained in the second step, and an average of 600 to 1700 5th instar mid-stage larvae are transferred out of each canned bottle; After 3 to 4 days, it enters the pupation stage;

   The fourth step is to take the sterile roll paper, cut it and fold it into several fan-shaped origami; when the larvae at the end of the 5th instar begin to change into pupae, place the origami on the rice seedlings so that the larvae can move to the gaps or folds of the origami to pupate ;Seal and tie tightly with a sterilized black cloth; after 2 days, take out each origami with a sterilized instrument; first place a sterile paper sheet on the bottom of the petri dish, and then use a sterilized instrument to collect the pupae on the origami to the paper in the petri dish On the sheet, moisten the sheet with double distilled water to maintain the developmental humidity of the pupae;

   Step 5: Take a number of petri dishes and place sterile roll paper on the bottom, moisten the sterile roll paper with double distilled water; transfer the pupae collected in step 4 to each petri dish, and place 50±5 per petri dish on average. 1 pupae to prepare for eclosion and mating and oviposition; take some insect cages and place sponges on the bottom, moisten the sponges with double distilled water; in each insect cage, place 2 pots in a diagonal line for spawning Put the rice seedlings with eggs into 2 petri dishes containing pupae; put each insect cage in an artificial climate box at 28°C±1°C and a relative humidity of 70-80%, and the pupae will emerge after 3-4 days, and the adult Mating in pitchers and laying eggs on rice seedlings in jars;

   Step 6: After 6 to 7 days of adult emergence, take out the rice seedlings from the insect cage, cut off the leaves of the rice seedlings carrying the egg mass with a sterilized instrument, and put them into a petri dish with moist filter paper on the bottom. 100-150 egg masses were obtained from the insect cage; the petri dish was fumigated with formaldehyde solution to aseptically treat the egg masses; the obtained egg masses were used for the next cycle of cultivation and rearing.
2. The indoor rice seedling precision large-scale breeding method of Chilo stem borer according to claim 1, is characterized in that, the ovum mass that the 3rd step contains the canned bottle of larvae, the 4th step contains pupa, or the 6th step obtains in Place it in an environment of 4°C±2°C for a preset time to control its development progress.
3. According to claim 1 and 2 described indoor rice seedling precision large-scale raising method of rice stem borer, it is characterized in that, in the first step, the specific process of cleaning and panning rice seed is:

   Put the rice seeds into the pot, pour tap water into the pot and submerge the rice seeds, and let it stand for a few minutes, so that the rice husks or dry rice seeds float on the water surface, while the full rice seeds sink to the bottom of the pot; the rice husks or dry rice seeds Pour the seeds together with the water into the drain basin, leaving only the full rice seeds at the bottom of the basin; repeat the above process until the full rice seeds sinking into the bottom of the basin are cleaned until the water becomes clear; the average weight of rice seeds in each pot is greater than 1000g , the final average weight of full rice seeds per pot is 93 ± 3% of the rice seeds put in.
4. According to the method for precise and large-scale breeding of rice seedlings indoors of Chilo borer according to claim 3, it is characterized in that, in the second step, the specific process of accelerating germination to obtain rice buds is:

   Take the rice seeds obtained in the first step, drain the rice seeds and put them into a pot, then seal the pot with a clean black plastic bag and tie the bag tightly, and place it at 28°C±1°C and a relative humidity of 70～ In an 80% artificial climate box, to accelerate the dew and break the chest of the seeds; turn the rice seeds once at 24±2 hours to make the temperature of the rice seeds uniform and ensure that the germination is neat; at 48±2 hours, the rice seeds have fully germinated, which is the rice buds.
5. The indoor rice seedling precision large-scale breeding method according to claim 4, is characterized in that, in the second step, the specific process of accelerating seedlings to obtain rice seedlings is:

   Wash the rice buds with double distilled water, and drain the water; place a sterile paper sheet on the bottom of the clean canning bottle as a carrier for the rice seeds to take root; use double distilled water to moisten the paper sheet, and sow the rice buds in the canning bottle In the process, seal with plastic wrap, prick holes with needles to ensure unobstructed oxygen, and place in an artificial climate box at 28°C±1°C and relative humidity of 70-80% to accelerate the emergence of rice seeds; Rinse the rice seedlings with water, and then pour the double distilled water to maintain the moisture required for the growth of the rice seedlings.
6. According to claim 5, the method for precise and large-scale breeding of rice seedlings of Chilo borer indoors is characterized in that, in the third step, tweezers sterilized with medical alcohol are used to burn them with an alcohol lamp and then naturally cooled for standby; the larvae are removed from the current canned bottle When transferring seedlings to a new jar, use tweezers to remove the seedlings from the current jar and place them on the first clean sheet, then pick out the larvae from these seedlings and place them on the second clean sheet , at last the larvae on the second piece of clean paper are directly poured on the rice seedlings in a new canning bottle, if any larvae escape from the clean paper at this time, they are picked on the rice seedlings in a new canning bottle with tweezers; after that, Seal the can with a sterilized black cloth and tie it tightly, and continue to place it in an artificial climate box at 28°C±1°C and a relative humidity of 70-80%; in the third step, place the larvae in an artificial climate with a consistent environment throughout in the box.
7. According to claim 5, the indoor rice seedling precision large-scale breeding method of Chilo borer is characterized in that, in the fourth step, when taking out each origami, the origami is put into a sterilized glass jar, and then subsequent operations are carried out; The sterilized instruments are medical tweezers sterilized with medical alcohol; the caliber of the petri dish used is 90mm; in the fifth step, the caliber of the petri dish used is 90mm; the sponge is moistened with 100±10mL double distilled water.
8. According to claim 5, the indoor rice seedling precision large-scale breeding method of Chilo borer is characterized in that, in the fifth step, the process of cultivating rice seedlings for laying eggs is: taking the rice buds obtained by accelerating germination in the second step , sow the seeds on the soil surface of the preset pot and cover with a thin layer of soil, cover the pot with a clean black plastic bag, and place it in an artificial climate chamber at 28°C±1°C and a relative humidity of 70-80% until the rice seedlings When the plant height reaches 10cm, the rice seedlings that are used to lay eggs are obtained; in the fifth step, a petri dish with a caliber of 60 mm is placed in the insect cage, and the petri dish is filled with honey with a volume ratio of 10 ± 1%. Disinfecting cotton balls with water; change cotton balls every two days.
9. According to claim 5, the method for precise and large-scale breeding of rice seedlings of Chilo borer indoors is characterized in that, in the sixth step, the specific process of fumigation is: placing the petri dish containing the egg mass on a container filled with formaldehyde solution at the bottom. Fumigate in a drying cylinder for 15 ± 1 min, and then take it out immediately; the sterilized instruments are sterilized scissors; the caliber of the petri dish used is 90 mm; the concentration of the formaldehyde solution is 35 ± 1% by volume.
10. The method for accurately and large-scale breeding of rice seedlings of Chilo borer indoors according to claim 5 is characterized in that, the rice seeds are japonica rice seeds; the caliber of the canned bottle is 6 cm and the height is 10 cm; the size of the insect cage is It is: 45cm×45cm×55cm; the number of canned bottles used in the third step is greater than 20 bottles.

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