CN114145269A

CN114145269A - Accurate large-scale feeding method for indoor rice seedlings of chilo suppressalis

Info

Publication number: CN114145269A
Application number: CN202111486141.5A
Authority: CN
Inventors: 徐鹿; 韩阳春; 赵钧; 刘宝生; 徐德进; 徐广春; 夏海波; 胡双女
Original assignee: Jiangsu Academy of Agricultural Sciences
Current assignee: Jiangsu Academy of Agricultural Sciences
Priority date: 2021-12-07
Filing date: 2021-12-07
Publication date: 2022-03-08
Anticipated expiration: 2041-12-07
Also published as: CN114145269B; WO2023103304A1

Abstract

The invention relates to a method for accurately feeding chilo suppressalis indoor rice seedlings on a large scale, which comprises the following steps: cleaning and elutriating rice seeds; sterilizing the obtained rice seeds; accelerating germination of rice seeds to obtain rice sprouts, and accelerating seedling to obtain rice seedlings; fine larva breeding is carried out by adopting rice seedlings; pupating and collecting pupae; mating and laying eggs for adults; collecting and processing the egg mass. The method accurately quantifies the operation steps of natural feed rice seedling preparation, larva feeding, pupation and pupation, adult mating and spawning and egg mass collection, forms a set of large-scale feeding method, and saves feeding time, cost and labor.

Description

Accurate large-scale feeding method for indoor rice seedlings of chilo suppressalis

Technical Field

The invention relates to a method for accurately feeding chilo suppressalis indoor rice seedlings on a large scale, and belongs to the technical field of artificial feeding of insects.

Background

According to the knowledge of the inventor, Chilo supressalis (Walker) belongs to Lepidoptera and Bombycidae, is a multi-feeding economic pest which is harmful to rice production, is distributed in Asia, middle east and southern Europe areas, particularly is seriously harmful to Yangtze river basin in China and southern rice area, because the popularization of hybrid rice and the reform of farming system, the generation amount of Chilo suppressalis is increased year by year, and the larva thereof eats rice stems and leaves through boring, thereby causing serious yield reduction. Chilo suppressalis can cause harm in various growth periods of rice. In the stage of tillering of rice, chilo suppressalis larvae can erode rice seedlings, so that the rice seedlings wither; in the booting stage and heading stage of the rice, chilo suppressalis can erode rice stalks to cause the rice to fail to be booting and produce withered booting and white ears; in the filling stage and the milk stage of the rice, chilo suppressalis larva erodes the rice stem to cause that the rice cannot be normally filled, semi-withered ears and insect-damaged plants appear, and the shriveled grains are increased. Can also damage caulis Zizaniae Caduciflorae, caulis Sacchari sinensis, jowar, semen Maydis, semen Tritici Aestivi, semen Setariae, rhizoma Sagittariae Sagittifoliae, semen Viciae Fabae, caulis et folium Brassicae campestris, Echinochloae crusgalli, herba Cymbopogonis Citrari, etc. According to statistics, the annual occurrence area of Chilo suppressalis in Chilo suppressalis is 1400 hectares, and the annual economic loss reaches 115 billion yuan. At present, the prevention and control of chilo suppressalis mainly depends on chemical pesticides to control the outbreak hazard of chilo suppressalis, but the chilo suppressalis has generated resistance to various common pesticides, so that the dosage and the dosage frequency are increased continuously, the prevention and control cost is increased, and the environmental management pressure is caused. In the process of developing prevention and treatment research and formulating striped rice borer control strategies aiming at striped rice borers, indoor stable population and large-scale artificial feeding of striped rice borers can provide stable insect sources at specific time and specific age for developing comprehensive prevention and treatment of striped rice borers, such as drug resistance monitoring and mechanism research, medicament screening, sex pheromones, transgenic insect-resistant rice, parasitic wasps and the like, so that the improvement of the striped rice borer artificial feeding method can promote the prevention and treatment of striped rice borers.

Since the 70 s of the last century, the research on the artificial feeding technology of chilo suppressalis has been carried out at home and abroad. The chilo suppressalis artificial feeding technology is divided into natural feed feeding and artificial feed feeding.

The natural feed breeding method has a wide application range by using rice seedlings or rice stems, and on the basis, the breeding methods of water bamboos, water chestnuts and the like are developed. In the existing natural feed breeding technology, cane shoots, water chestnuts and rice are used as natural feeds, the influence of the three natural feeds on the growth development and the fertility of the chilo suppressalis is analyzed, and the chilo suppressalis can complete the generation development and the propagation (Mengxianxia, Wufucming, Gashiwu. the technical research of breeding the chilo suppressalis by using the cane shoots, the water chestnuts and the rice. insect knowledge 2003, 40 (5): 469-.

The natural feed not only serves as a food source of the chilo suppressalis, but also provides a habitat for the chilo suppressalis, is limited by the prior feed preservative technology, is easy to rot and deteriorate or mildew for host plants for feeding the chilo suppressalis, and the rotten and deteriorated or mildewed host plants need to be replaced in time in the feeding process, so that larvae are malnourished and infected with germs to die, the population base number of the chilo suppressalis is reduced, and the chilo suppressalis is not beneficial to realizing large-scale feeding; the problems of unbalanced number and gender of chilo suppressalis, inconsistent growth progress and the like (Shang juzhen, Wang silver shu, Zhonghua, Chilo suppressalis breeding method research, insect academic report, 1979, 22 (2): 164 + 167, Luyurong, Sujian Kun, Jichumin, Liuqin, Zhang Chun Mei, Liuhua A. the indoor breeding method of rice chilo suppressalis is primarily explored, Anhui agricultural science 2003, 31 (2): 270 + 271, Zheng Fushan, Qiangcheng Quiki, Dong hongxia, Shenyu, the collection and indoor breeding method of chilo suppressalis, Anhui agricultural science 2009, 37 (17): 7837 + 7838) can also occur in the natural feed breeding process.

The technology for breeding chilo suppressalis by using artificial feed begins with the development of stone-well feed and kettle wild feed. The artificial feed for the chilo suppressalis is researched successively, and the problem of the insect source demand of the chilo suppressalis test is solved to a certain extent through the preliminary research of the practical artificial feed for the chilo suppressalis in 1984, but the feed has single component and cannot meet the generation circulation of the chilo suppressalis; the feed is easy to decay, and the feed is frequently replaced. On the basis of the research, the artificial feed of the chilo suppressalis is further optimized, the survival rate, the pupation rate and the emergence rate of the chilo suppressalis are improved, but the growth rate of the chilo suppressalis is slow and inconsistent, and the scale feeding of the chilo suppressalis (huyang, Zheng Youli, Cao Lian, Fu Qiang, large-scale simple and convenient feeding of the chilo suppressalis by using the semi-artificial feed is influenced, Chinese rice science 2013, 27(5) 535. the feeding effect of key factors of the chilo suppressalis artificial feed is optimized and optimized according to the formula.2008, 45(2) 310. mangnolia officinalis 314. plum wave, Han Lanzhi, Peng. chilo suppressalis artificial feeding technology.application of the insect science 2015 52(2) 498. 2015 503).

The artificial feed cannot replace the habitat of the natural feed, and the Chilo suppressalis bred by the artificial feed is different from the Chilo suppressalis bred by the natural feed, so that the test result has deviation. Raw materials of the artificial feed are relatively difficult to obtain, and the feed cost is increased; the preparation process is complicated, a feed processing instrument is required, and the labor cost is high; the artificial feed is preserved, but needs low-temperature refrigeration, which increases the equipment cost.

Through search, the invention patent applications with application numbers of CN201910423574.2 and CN110024751A disclose a chilo suppressalis breeding device and a breeding method, and although the chilo suppressalis is bred by using natural feed, the device is complex to manufacture, complex to operate and the like. The invention patent application with application No. CN201510086108.1 and application publication No. CN104604814A discloses a method for efficiently feeding chilo suppressalis, which simplifies a feeding device, but still has the disadvantages that the operation complexity and the feeding scale have certain limitations and the like.

The invention patent application with application number CN201210068466.6 and application publication number CN102578051A discloses a method for artificially feeding Chilo suppressalis indoors, which mainly uses a small self-sealing bag commonly used in a laboratory as a Chilo suppressalis egg bed, not only is the material acquisition quite simple, but also the specific operation of egg collection is more simplified, and meanwhile, a plastic box with the size of 20x15x15(cm) is used as a container, so that the occupied space of the whole spawning device is smaller than that of rice seedlings. Although the technical scheme adopts the rice seedlings as the natural feed, no treatment means for overcoming the defect that the rice seedlings are easy to rot and deteriorate or mildew is provided, and no treatment means for overcoming the defect that the development progress of the chilo suppressalis is inconsistent are provided, more importantly, the method does not pay attention to how to realize the large-scale feeding of the chilo suppressalis, but focuses on simplifying the feeding process, and the large-scale feeding of the chilo suppressalis is difficult to realize only by the technical scheme, so that the sufficient number of the chilo suppressalis population cannot be provided.

The invention patent application with the application number of CN201911073784.X and the application publication number of CN110604102A discloses a chilo suppressalis standardized feeding and management method, which comprises the processes of artificial feed preparation, larva 36-day feeding and adult feeding. Taking 5 days as the period for collecting eggs in the indoor population, taking 4 days as the period for collecting pupae, and taking 36 days as the larva feeding period for inoculating single batch of egg blocks to the pupation stage. A novel management system for multi-batch overlapping circulating feeding is established through the standardized management of different insect state operation flows. However, the technical scheme adopts artificial feed instead of natural feed, cannot utilize the advantages of the natural feed, has complicated operation steps, even needs to complete the whole feeding process of inoculating the egg mass to pupate on a sterile operation table, has higher requirements on the operation environment and operators, and is not beneficial to realizing large-scale feeding.

In a word, the method for feeding the chilo suppressalis by using the natural feed has the advantages that the existing artificial feed feeding technology cannot achieve, and the improvement of the technology for feeding the chilo suppressalis by using the natural feed is urgently needed, so that the feeding operation steps are simplified, the cost and the labor are saved, and the insects for scale tests can be provided.

Disclosure of Invention

The main purposes of the invention are: the method can accurately quantify the Chilo suppressalis breeding flow, save time and breeding cost, solve the problems of rotting and deterioration or mildew formation of the rice seedlings of natural host plants, reduce the death rate of Chilo suppressalis larvae, improve the pupation rate, ensure the indoor population scale of the Chilo suppressalis and can continue to multiply.

The technical scheme for solving the technical problems of the invention is as follows:

an accurate large-scale feeding method for indoor rice seedlings of chilo suppressalis is characterized by comprising the following steps:

firstly, cleaning and elutriating rice seeds; sterilizing the obtained rice seeds;

the disinfection process comprises the following steps: uniformly mixing a sodium hypochlorite solution and tap water according to the volume ratio of 1 +/-0.2: 100, putting the rice seeds into the mixture, soaking the rice seeds for 24 +/-2 hours, and then washing the rice seeds with tap water until the sodium hypochlorite is completely washed; soaking the rice seeds in tap water for 24-48 hours, and then washing the rice seeds by double-distilled water; the content of active chlorine in the sodium hypochlorite solution is 9 +/-1% by mass;

secondly, taking the rice seeds obtained in the first step, accelerating germination to obtain rice sprouts, and accelerating seedling to obtain rice seedlings; the obtained rice seedlings are placed in sterilized can bottles, and 50 +/-5 g of rice sprouts are averagely used in each can bottle; when the rice seedlings grow to be more than 3cm and not more than 4cm, using the can bottle for the third step;

thirdly, placing the chilo suppressalis egg blocks subjected to aseptic treatment on the rice seedlings in the cans obtained in the second step, and placing 5-7 chilo suppressalis egg blocks in each can on average; the aseptic treatment is fumigation by formaldehyde solution; the average number of eggs contained in each egg block is 200-300, and the average number of eggs contained in each can bottle is 1000-2100; sealing and fastening the can bottle by using sterilized black cloth, and placing the can bottle in an artificial climate box at the temperature of 28 +/-1 ℃ and the relative humidity of 70-80%; adding double distilled water into the can for 1 time every 2 days, and adding water along the wall of the can to moisten the roots of the rice seedlings; the time from laying the egg mass to hatching out 1-instar larvae is 2-4 days; 6-7 days are needed for the larvae from 1 st to 2 nd; when the larvae grow to the middle and later 2-year old, transferring the larvae in each can bottle to the rice seedlings in the new can bottle obtained in the second step, and averagely transferring out 900-2000 larvae in the middle 2-year old from each can bottle; 3-4 days are needed for the larvae of 2 th instar to grow into the larvae of 3 rd instar; when the larvae grow to the final stage of 3-year larvae, transferring the larvae in each can bottle to the rice seedlings in the new can bottle obtained in the second step, and averagely transferring out 800-1900 larvae in the final stage of 3-year larvae in each can bottle; 3-4 days are needed for the larvae from the last stage of 3 instars to the larvae from the last stage of 4 instars, the larvae in each can bottle are transferred to the rice seedlings in the new can bottle obtained in the second step when the larvae are at the 4 instars, and 700-1800 larvae of 4 instars are averagely transferred out from each can bottle; the development of the larvae from the late stage of 4 th instar to the late stage of 5 th instar requires 2-3 days, when the larvae grow to the middle stage of the larvae of 5 th instar, the larvae in each can bottle are transferred to the rice seedlings in the can bottle obtained in the new second step, and on average, each can bottle is transferred to 600-1700 larvae in the middle stage of 5 th instar; continuously feeding, wherein the larvae of the last 5 th instar enter a pupation stage after 3-4 days;

fourthly, taking the sterile roll paper, cutting the sterile roll paper and folding the sterile roll paper into a plurality of fan-shaped folded papers; when the larva begins to change to the pupa form at the later 5 th instar, the folded paper is placed on the rice seedling so that the larva moves to the gap or the folding part of the folded paper to pupate; sealing with sterilized black cloth and fastening; after 2 days, taking out each folded paper by using a sterilized instrument; laying sterile paper sheets at the bottom of a culture dish, collecting pupas on folded paper onto the paper sheets in the culture dish by using a sterilized instrument, and wetting the paper sheets by using double-distilled water to keep the development humidity of the pupas;

fifthly, taking a plurality of culture dishes, paving sterile roll paper at the bottom of the culture dishes, and wetting the sterile roll paper by double distilled water; transferring the pupae collected in the fourth step to each culture dish, and averagely placing 50 +/-5 pupaes in each culture dish for eclosion and mating and oviposition; taking a plurality of insect cages, paving sponges on the bottoms of the insect cages, and wetting the sponges with double distilled water; in each insect cage, 2 rice seedlings for spawning are placed in a diagonal line mode, and then 2 pupa-containing culture dishes are placed; putting each insect cage into an artificial climate box with the temperature of 28 +/-1 ℃ and the relative humidity of 70-80%, enabling pupae to eclose for 3-4 days, mating eclose adults in the insect cages, and laying eggs on canned rice seedlings;

sixthly, taking out rice seedlings from the insect cage after eclosion of adults for 6-7 days, shearing off rice seedling leaves loaded with egg blocks by using a sterilized instrument, and putting the rice seedling leaves into a culture dish paved with wet filter paper at the bottom, wherein 100-150 egg blocks are obtained per insect cage on average; fumigating the culture dish with formaldehyde solution to aseptically treat the egg mass; the obtained egg mass is used for the next period of cultivation and feeding.

In the method, the rice seeds are disinfected, so that the phenomenon that rice seedlings are rotten and go bad or mildew can be avoided, the problem that natural feed is easy to rot is solved, and chilo suppressalis larvae are prevented from dying in a large area due to the influence of rotten and go bad or mildew of the rice seedlings; the rice bud consumption of each can bottle is strictly limited, which is beneficial to the accurate quantification of the feeding process and lays a foundation for the subsequent large-scale feeding of chilo suppressalis; the time node and the rice seedling transferring operation during the period from the hatching of the egg mass to the larva at the late 5 th age are accurately limited, so that not only is the accurate quantification of the feeding process realized, but also the high survival rate of the chilo suppressalis larva can be ensured through the fine operation, and the feeding scale with a large base number is formed; in the pupa melting and collecting processes, the pupa can be smoothly collected by adopting a simple carrier of fan-shaped folded paper, so that the feeding process is simpler and easier; in the process of mating and spawning of the eclosion adult, the rice seedlings are used as natural substrates for the adult to spawn, so that the spawning rate is improved, and the subsequent large-scale breeding is facilitated. The feeding method can be continuously carried out in a circulating and reciprocating manner, so that a multi-batch large-scale insect source with consistent development progress (insect state, age, individual size, health condition, sensitivity and the like) is continuously provided for the prevention and treatment research of the chilo suppressalis, the repeated accuracy of the test is ensured, and the research work cycle is shortened; the process is simple, and only 1 person is needed to complete all the feeding operations.

The technical scheme of the invention is further perfected as follows:

preferably, the third step of the canned bottle containing the larvae, the fourth step of the culture dish containing the pupae, or the egg mass obtained in the sixth step are placed in an environment with the temperature of 4 +/-2 ℃ for a preset time to control the development progress of the egg mass.

After the optimal scheme is adopted, the growth and development of the chilo suppressalis in the egg stage, the larva stage and the pupal stage can be further controlled by adjusting the temperature, so that the development progress consistency is higher, and the time window requirement of a corresponding prevention and treatment research project on the insect source development stage can be met.

Preferably, in the first step, the specific process of washing and elutriating rice seeds is as follows:

putting the rice seeds into a basin, filling tap water into the basin and submerging the rice seeds, standing for several minutes to enable the rice husks or the shriveled rice seeds to float on the water surface and the full rice seeds to sink at the bottom of the basin; pouring rice husks or shriveled rice seeds and water into a draining basin, and only remaining full rice seeds at the bottom of the basin; repeating the process until the full rice seeds sinking into the bottom of the basin are cleaned until the water becomes clear; the weight of the rice seeds put in each pot is more than 1000g on average, and the weight of the filled rice seeds obtained in each pot is 93 +/-3 percent of the weight of the put rice seeds.

After the preferred scheme is adopted, the specific technical details of the first step can be further optimized, so that the first step is simplified; meanwhile, the amount of the used rice is large, so that the rice seedlings can be continuously provided as natural feed in the follow-up process.

Preferably, in the second step, the specific process of accelerating germination to obtain rice sprouts is as follows:

taking the rice seeds obtained in the first step, draining water of the rice seeds, putting the rice seeds into a basin, then sealing the basin by using a clean black plastic bag, binding the mouth of the basin, and placing the basin in an artificial climate box at the temperature of 28 +/-1 ℃ and the relative humidity of 70-80% to accelerate the whitening and chest breaking of the seeds; turning the rice seeds for 1 time at 24 +/-2 hours to ensure that the temperature of the rice seeds is uniform and consistent and the germination is uniform; the rice seeds fully germinate at 48 +/-2 hours, and the rice sprouts are obtained.

Preferably, in the second step, the specific process of accelerating the seedling to obtain the rice seedling is as follows:

cleaning the rice sprouts with double distilled water, and pouring the water to dry; laying aseptic paper sheets at the bottom of the clean can bottle as a carrier for rice seeds to root; wetting paper sheets by using double distilled water, sowing rice sprouts in a can bottle, sealing the can bottle by using a preservative film, pricking holes by using needles to ensure that oxygen is smooth, and placing the can bottle in an artificial climate box at the temperature of 28 +/-1 ℃ and the relative humidity of 70-80% to accelerate the emergence of rice seeds; washing the rice seedlings with 100 +/-10 mL of double distilled water every 48 +/-2 hours, and then pouring the double distilled water to maintain the water required by the growth of the rice seedlings.

After the preferred scheme is adopted, the specific technical details of the second step can be further optimized, so that the method is simplified, a large number of rice seedlings with consistent growth progress can be cultivated in each batch, and sufficient natural feed is provided for subsequent feeding.

Preferably, in the third step, forceps disinfected by medical alcohol are adopted, and the forceps are burned by an alcohol lamp and then naturally cooled for standby; when transferring the larvae from the current can bottle to the rice seedlings in a new can bottle, clamping the rice seedlings from the current can bottle by using tweezers and placing the rice seedlings on a first piece of clean paper, picking out the larvae from the rice seedlings and placing the larvae on a second piece of clean paper, and finally directly pouring the larvae on the second piece of clean paper onto the rice seedlings in the new can bottle, and if the larvae escape from the clean paper, picking the larvae onto the rice seedlings in the new can bottle by using the tweezers; then, sealing and fastening the can bottle by using sterilized black cloth, and continuously placing the can bottle in an artificial climate box with the temperature of 28 +/-1 ℃ and the relative humidity of 70-80%; in the third step, the larvae are kept in a climatic chamber where the environment is kept constant throughout the rearing process.

After the preferable scheme is adopted, the specific technical details of the third step can be further optimized, and the larval can be better ensured not to die in a large area due to seedling replacement; keeping the feeding environment consistent can ensure that the larvae enter all development stages in order.

Preferably, in the fourth step, when taking out each folded paper, putting the folded paper into a sterilized glass jar, and then performing subsequent operation; the sterilized instrument is medical forceps sterilized by medical alcohol; the caliber of the culture dish is 90 mm; in the fifth step, the caliber of the culture dish is 90 mm; the sponge was wetted with 100 + -10 mL double distilled water.

After the preferred scheme is adopted, the specific technical details of the fourth step and the fifth step can be further optimized to achieve better technical effects.

Preferably, in the fifth step, the process of breeding rice seedlings for oviposition is: taking rice sprouts obtained by germination accelerating in the second step, sowing the rice sprouts on the surface layer of soil of a preset pot and covering a layer of thin soil, covering the pot with a clean black plastic bag, and culturing in an artificial climate chamber with the temperature of 28 +/-1 ℃ and the relative humidity of 70-80% until the plant height of rice seedlings reaches 10cm, thus obtaining the rice seedlings for spawning; fifthly, a culture dish with the caliber of 60mm is also placed in the insect breeding cage, and the culture dish is filled with sterilized cotton balls dipped with honey water with the volume ratio of 10 +/-1%; the cotton ball was replaced every two days.

After the preferable scheme is adopted, additional specific technical details in the fifth step can be further optimized, wherein the rice seedling cultivation process for spawning is accurately limited, and the spawning environment can be better provided; the honey water can supplement nutrition for eclosion adults and promote the adults to lay eggs, and the adults can be prevented from falling into the honey water and dying by dipping the adults in the honey water through a sterilized cotton ball.

Preferably, in the sixth step, the specific process of fumigation comprises: putting the culture dish containing the egg mass into a drying cylinder with formaldehyde solution at the bottom for fumigating for 15 +/-1 min, and then immediately taking out; the sterilized instrument is sterilized scissors; the caliber of the culture dish is 90 mm; the concentration of the formaldehyde solution is 35 +/-1 percent by volume.

After the preferred scheme is adopted, the specific technical details of the sixth step can be further optimized, wherein the parasitic bacteria on the surface of the egg mass can be killed in the fumigating process, and the survival rate of the larvae in the subsequent feeding process is improved.

Preferably, the rice seeds are japonica rice seeds; the caliber of the can bottle is 6cm and the height of the can bottle is 10 cm; the insect cage has the following dimensions: 45cm × 45cm × 55 cm; the number of the cans adopted in the third step is more than 20.

The method accurately quantifies the operation steps of natural feed rice seedling preparation, larva feeding, pupation and pupation, adult mating and spawning and egg mass collection, forms a set of large-scale feeding method, and saves feeding time, cost and labor.

Compared with the prior art, the invention has the main advantages that: (1) corresponding disinfection and antibacterial measures are adopted in the whole process of egg-larva-pupa-imago-egg, so that the infection of pathogenic bacteria is comprehensively controlled, and the generation development and the population scale are ensured. (2) The preparation of the natural feed comprises the steps of cleaning, sterilizing, accelerating germination and sowing of rice seeds, effectively controls rice seedlings to rot or go mouldy, provides sufficient nutrition for larvae, optimizes the inhabitation environment of the larvae, reduces the replacement frequency of the natural feed and improves the survival rate of the larvae. (3) The novel pupation and pupation collecting method has the advantages that the old larvae are drilled into the folding positions of the folding paper to form cocoons and pupate, a safe and closed pupation place with proper humidity is provided, the pupation quantity can be increased, the pupation weight can be increased, the folding paper can be used as a unit for storage and transportation, the pupation finding is simple and convenient, and the method is suitable for large-scale subculture. (4) The egg laying environment of the chilo suppressalis adults is optimized, and the adult chilo suppressalis can mate and lay eggs in the sterile constant-temperature constant-humidity environment by adopting the insect rearing cage suitable for the chilo suppressalis, so that the egg laying amount is increased; the adoption of the natural feed rice seedlings provides an oviposition matrix for reducing the natural survival mode of chilo suppressalis, and the oviposition rate is improved. (5) The chilo suppressalis bred according to the method of the invention has close biological characteristics with the chilo suppressalis in nature, and the research result deviation of related research is small.

Drawings

FIG. 1 is a schematic diagram of the preparation of a Chilo suppressalis natural feed in example 1 of the present invention.

FIG. 2 is a schematic diagram of feeding larvae of japonica rice seedlings of chilo suppressalis in example 2 of the present invention.

FIG. 3 is a schematic diagram of the pupation and pupation of Chilo suppressalis in example 3 of the present invention.

FIG. 4 is a schematic diagram of the mating and oviposition of Chilo suppressalis adults in example 4 of the present invention.

FIG. 5 is a schematic diagram of the collection and preservation of chilo suppressalis egg masses in example 5 of the present invention.

Detailed Description

The present invention will be described in further detail with reference to examples. The invention is not limited to the examples given. The reagents related to the present invention are all commercially available products.

Example 1 Chilo suppressalis Natural feed preparation

In the embodiment, the natural feed of the chilo suppressalis uses a japonica rice variety Nanjing 46 which is a main pushed variety in Jiangsu province and is widely planted in cities and counties in the Jiangsu province. The invention can select different rice varieties as natural feed, and the local rice varieties are selected, so that local materials can be conveniently obtained, and the invention can also accord with the actual living environment of local chilo suppressalis.

(1) 1500g of Nanjing 46 seeds are weighed by a balance, the weight of the japonica rice seeds can ensure the large-scale propagation needs of the chilo suppressalis, and host food and habitat can be provided for continuous breeding.

Putting the weighed south japonica 46 seeds into a 10L plastic basin, pouring 5L of tap water into the plastic basin by using a measuring cup, forcibly stirring the japonica rice seeds in the tap water, standing for several minutes, pouring residual rice husks or shriveled rice seeds into a draining basin, and leaving full rice seeds at the bottom of the basin. Pouring 5L of tap water into a plastic basin by using a measuring cup, stirring the japonica rice seeds forcibly, and washing out residual rice husks or shrivelled rice seeds. This panning step was repeated 5 times in total to remove the rice husk or shrivelled seeds.

The weight of the japonica rice seeds settled at the bottom of the plastic basin is about 1400g, 3L of tap water is poured into the plastic basin by using a measuring cup to soak the japonica rice seeds for subsequent disinfection.

(2) 0.03L of sodium hypochlorite solution (containing 9% of active chlorine by weight, the same below) is poured into 3L of tap water according to the ratio of the sodium hypochlorite solution to the tap water of 1:100, the mixture is fully stirred and uniformly mixed, the mixed solution of the sodium hypochlorite and the tap water for soaking japonica rice seeds is poured after 24 hours, and the rice seeds are fully cleaned by the tap water until the pungent smell of the sodium hypochlorite cannot be smelled.

Pouring 3L of tap water into the plastic basin by using the measuring cup to continuously soak the japonica rice seeds, wherein the standard for fully soaking the seeds is that the rice husks are transparent, the rice grains are white and visible, the rice grains with the rice husks removed are easy to break and have no noise, and when the rice grains are crushed by hands, the seeds are proved to absorb enough water. Pouring tap water for soaking the japonica rice seeds after 24 hours.

2L of double distilled water is poured into the measuring cup, the soaked japonica rice seeds are cleaned, the double distilled water is poured after the seeds are fully cleaned, the cleaning process is repeated for 3 times, the operation step can be used for washing away trace chemical components in tap water, and the seeds are prevented from budding and the chilo suppressalis are prevented from being poisoned.

(3) After the rice seeds are drained, the plastic basin is sealed by a black plastic bag and the opening of the plastic bag is tightly bound, and the black plastic bag has good heat preservation effect and is beneficial to the sprouting of the japonica rice seeds. Placing in an RGC-1000C artificial climate box (combined fertilizer right department instruments and equipment Co., Ltd.), setting the temperature at 28 ℃, the relative humidity at 80%, and the illumination: dark 16 h: and 8 h. In the process of accelerating germination, the temperature is required to be kept not lower than 25 ℃ to prevent high-temperature burning, and the seeds lose germination capacity at the temperature of more than 40 ℃. Turning over the japonica rice seeds for 1 time every 24 hours, fully and uniformly mixing residual moisture at the bottom of the plastic basin and the seeds during turning over, ventilating and increasing oxygen, and accelerating the vigorous growth of seed buds.

(4) After 48h, the whole japonica rice seeds bud, 2L double-distilled water is poured into the measuring cup to gently clean the germinated rice seeds, the process is repeated for 2 times, the rice buds are not broken during cleaning, and the water is fully drained. The commercially available sterile roll paper is cut into paper sheets with the length of 13.9cm and the width of 10.2cm and is used as a substrate for growth of japonica rice seeds. Placing the paper into the bottom of 500mL can with diameter of 6cm and height of 10cm, pouring 10mL double distilled water into a measuring cylinder for wettingThen, 50g of the soaked japonica rice sprouts were weighed and sown in a can bottle. Cutting common preservative film to the size of the can mouth, sealing the can mouth with the common preservative film, and sealing the can mouth with the common preservative film by 0^#10 ~ 15 insect needles prick the hole on the plastic wrap, guarantee that oxygen is unobstructed. 1400g of japonica rice sprouts can be planted with 28 bottles of rice seedlings, placed in an RGC-1000C artificial climate box (combined fertilizer Right department instruments Co., Ltd.), set at 28 ℃, relative humidity 80%, illumination: dark 16 h: and 8 h. In order to ensure the emergence of the rice sprouts, the temperature is not lower than 25 ℃, the rice sprouts grow to 1-2 cm after 72 hours, and 5mL of double-distilled water is poured along the inner wall of the bottle to prevent the rice seedlings from withering. In the seedling emergence process, 100mL of double distilled water is used for washing the rice seedlings every 48h, and then the double distilled water is poured, so that the water required by the growth of the rice seedlings can be ensured.

The above process is shown in fig. 1.

Example 2 Chilo suppressalis larva rearing

(1) First instar larva rearing

When japonica rice seedlings in the can bottle grow to be larger than 3cm, chilo suppressalis aseptic egg blocks with the same size are placed in the rice seedlings in the can bottle, the number of the egg blocks is 5, the placed egg blocks are egg blocks to be hatched into larvae, and the judgment standard is that the egg blocks are black and are placed in an artificial climate box for 2 days. The number of the canned bottles for placing the egg masses is 28. The size of the egg mass used in the embodiment is 15mm in length and 2mm in width, the number of eggs in the egg mass is 200-300, the number of eggs in a can bottle is 1000-1500, and the total number of eggs in one generation is 28000-42000. The can bottle was sealed with black cloth and secured with rubber band (as shown in fig. 2). Placing in an RGC-1000C artificial climate box (combined fertilizer right department instruments and equipment Co., Ltd.), setting the temperature at 28 ℃, the relative humidity at 80%, and the illumination: dark 16 h: and 8 h. Hatching 1-instar larvae after the egg mass is hatched for 2 days, adding 10mL of double-distilled water along the wall of the can once every 48h in the growth stage of the larvae, and wetting the roots of the rice seedlings. During the 1 st instar larva rearing stage, japonica rice seedlings required in larva rearing were prepared according to example 1 while maintaining the same number of flasks.

(2) Rearing of second instar larva

The growth of 1 st larva of chilo suppressalis to 2 nd larva needs 7 days, and japonica rice seedlings do not need to be replaced in the stage, and only enough moisture is required for the growth of the rice seedlings. In the middle and later 2-year-old period, the food intake begins to increase along with the increase of the insect body, and the japonica rice seedlings need to be replaced. The tweezers are taken out of 75% disinfecting alcohol, an alcohol lamp is ignited, the tweezers are placed above the flame of the alcohol lamp to be burnt, the tweezers are disinfected and sterilized, and then the tweezers are placed on sterile roll paper to be naturally cooled. 2 sheets of A4 copy paper are laid on the test bed, one sheet is used for temporarily placing japonica rice seedlings clamped out from a can bottle, 2-instar larvae inhabit among the japonica rice seedlings, and the other sheet is used for temporarily placing 2-instar larvae picked out from the japonica rice seedlings. Directly pouring 2-instar larvae on the A4 copy paper into can bottles containing fresh japonica rice seedlings, picking the escaped 2-instar larvae into the can bottles by using tweezers, and transferring 900-1400 2-instar larvae out of each can bottle. After the transfer, the container is sealed by black cloth, fastened by a rubber band, and placed in an RGC-1000C artificial climate box (combined fertilizer right department instruments and equipment Co., Ltd.), the set temperature is 28 ℃, the relative humidity is 80%, and the illumination is as follows: dark 16 h: and 8 h.

Used black cloth of sealing is raised to 1 age larva, places in the plastics basin, and in 2L running water plastics basins were poured into to the quantity cup, according to sodium hypochlorite solution: the required amount of sodium hypochlorite solution is 20mL, and the solution is poured into a plastic basin and mixed with tap water uniformly. After 24h of soaking, the solution was rinsed thoroughly under tap water until no offensive smell of sodium hypochlorite was detected. Dripping several drops of cold water degreasing detergent into a plastic basin, soaking and cleaning the sealed black cloth in tap water, wringing out the black cloth after cleaning, and drying in a model WGL-125B electric heating blowing drying oven (Tester instruments, Inc. of Tianjin) at 50 ℃. The used tin bottle with 500mL of tap water is fed to the 1 st larva by pouring a measuring cup, and the weight ratio of the tap water to the sodium hypochlorite solution is as follows: water 1:100, the required amount of sodium hypochlorite solution 5mL, poured into a can bottle and mixed with tap water. After 24h of soaking, the solution was rinsed thoroughly under tap water until no offensive smell of sodium hypochlorite was detected. 1-2 drops of cold water degreasing detergent are dripped into the can bottle, the can bottle is cleaned under tap water, and foams are washed out. And (3) placing the can bottle in a digital display air-blast drying oven with the model GZX-9140ME for high-temperature baking at the temperature of 200 ℃ for 6h, and naturally cooling after baking. Then placing the sealing black cloth and the can bottle in an artificial climate box, turning on an ultraviolet lamp, sterilizing for 30 minutes under ultraviolet rays, blowing for 10 minutes, and taking out the sealing black cloth and the can bottle for later use.

(3) Feeding third instar larva

The method comprises the steps of taking out tweezers from 75% sterile alcohol, burning the tweezers above the flame of an alcohol lamp, sterilizing the tweezers, and naturally cooling the tweezers on sterile roll paper. 2 sheets of A4 copy paper are laid on the test bed, one sheet is used for temporarily placing japonica rice seedlings clamped out from a can bottle, 3-instar larvae inhabit among the japonica rice seedlings, and the other sheet is used for temporarily placing 3-instar larvae picked out from the japonica rice seedlings. Directly pouring 3-year-old larvae on the A4 copy paper into can bottles containing fresh japonica rice seedlings, picking the escaped 3-year-old larvae into the can bottles by using tweezers, and transferring out 800-1300 3-year-old larvae from each can bottle. After the transfer, the container is sealed by black cloth, fastened by a rubber band, and placed in an RGC-1000C artificial climate box (combined fertilizer right department instruments and equipment Co., Ltd.), the set temperature is 28 ℃, the relative humidity is 80%, and the illumination is as follows: dark 16 h: and 8 h. Similarly, the sealed black cloth and the can were sterilized for use according to the procedure of (2) in example 2. Japonica rice seedlings required for larval rearing were prepared according to the procedure in example 1 while keeping the same number of bottles.

(4) Rearing of four instar larvae

And (4) entering a binge eating period at the later stage of 3 instar until the end of the later stage of 4 instar larvae, and replacing rice seedlings for 1 time from the later stage of 3 instar larvae to the end of 4 instar larvae. Taking out the tweezers from 75% sterilized alcohol, burning above the flame of alcohol lamp, sterilizing the tweezers, and naturally cooling on sterile roll paper. 2 sheets of A4 copy paper are laid on the test bed, one sheet is used for temporarily placing japonica rice seedlings clamped out from a can bottle, 4-instar larvae inhabit among the japonica rice seedlings, and the other sheet is used for temporarily placing 4-instar larvae picked out from the japonica rice seedlings. Directly pouring the larvae of 4 th instar on the A4 copy paper into can bottles containing fresh japonica rice seedlings, picking the escaped larvae of 4 th instar into the can bottles by using tweezers, and transferring 700-1200 larvae of 4 th instar out of each can bottle. After the transfer, the container is sealed by black cloth, fastened by a rubber band, and placed in an RGC-1000C artificial climate box (combined fertilizer right department instruments and equipment Co., Ltd.), the set temperature is 28 ℃, the relative humidity is 80%, and the illumination is as follows: dark 16 h: and 8 h. Similarly, the sealed black cloth and the can were sterilized for use according to the procedure of (2) in example 2. Japonica rice seedlings required for larval rearing were prepared according to the procedure in example 1 while keeping the same number of bottles.

(5) Rearing of five-instar larvae

After the larvae of late 4 th instar enter the larvae of 5 th instar, the food intake is reduced, and cocooning and pupating are started. Replacing rice seedling for 1 time in the middle age of 5 years, taking out the tweezers from 75% sterile alcohol, burning above the flame of alcohol lamp, sterilizing the tweezers, and naturally cooling on sterile roll paper. 2 pieces of A4 copy paper are laid on the test bench, one piece is used for temporarily placing japonica rice seedlings clamped from a can bottle, 5-instar middle-stage larvae inhabit among the japonica rice seedlings, and the other piece is used for temporarily placing 5-instar middle-stage larvae picked from the japonica rice seedlings. Directly pouring 5-instar middle-stage larvae on A4 copy paper into a can bottle containing fresh japonica rice seedlings, picking escaped 5-instar middle-stage larvae into the can bottle by using tweezers, and transferring 600-1100 5-instar middle-stage larvae out of each can bottle. After the transfer, the bag is sealed by black cloth and is fastened by a rubber band. Using 6 sheets (13.9 cm long and 10.2cm wide) cut from sterile roll paper, folding into fan-shaped folded paper, placing on the rice seedling, sealing with black cloth, and fastening with rubber band. Placing in an RGC-1000C artificial climate box (combined fertilizer right department instruments and equipment Co., Ltd.), setting the temperature at 28 ℃, the relative humidity at 80%, and the illumination: dark 16 h: and 8 h. The larvae bred in the embodiment are placed in an environment with constant temperature and humidity and consistent illumination in the whole process, and the larvae at the end of 5 th age enter a pupation stage regularly.

Example 3 Chilo suppressalis pupation and pupation

(1) Chilo suppressalis pupation (as shown in figure 3)

The chilo suppressalis 5-instar larvae enter a pupation stage after 3 days, the larvae climb to gaps of folded paper to pupate, the larvae at the late 5-instar stage hardly eat food, and the larvae only need to wait for pupation at the stage. In a glass jar (aperture 18cm height 9cm) prepared to place the origami, 2L of tap water was poured into the measuring cup, and the contents of sodium hypochlorite solution: the required amount of sodium hypochlorite solution is 20mL, and the solution is poured into a glass jar and mixed with tap water uniformly. After 24h of soaking, the solution was rinsed thoroughly under tap water until no offensive smell of sodium hypochlorite was detected. Dripping several drops of cold water degreasing detergent into the glass jar, cleaning the glass jar under tap water, and washing to remove foams. And (3) placing the glass jar in a digital display air-blast drying oven of GZX-9140ME for high-temperature baking at the temperature of 200 ℃ for 6h, and naturally cooling after baking. Then the glass jar is sterilized by ultraviolet rays for 30 minutes under an ultraviolet lamp in a climatic chamber, and is taken out for standby after being blown by wind for 10 minutes. Putting a 90mm glass culture dish into a plastic basin, pouring tap water into the plastic basin, dripping a plurality of drops of cold water degreasing detergent, fully stirring the tap water, cleaning the glass culture dish by using a brush, naturally drying the glass culture dish after fully cleaning, wrapping the glass culture dish by 10 newspapers in a group, putting the glass culture dish into a D-1 type automatic steam sterilization pot (Beijing hair En Corp. Co., Ltd.) for sterilization at the temperature of 120 ℃ for 20min, and drying the glass culture dish in a model WGL-125B electrothermal blowing drying oven (Tester instruments Co., Tianjin City) after sterilization at the temperature of 50 ℃. After drying, the culture dish can be taken out for standby, and the operation can reuse the culture dish, thereby reducing the cost.

(2) Chilo suppressalis pupa collecting (as shown in figure 3)

Taking out the tweezers from 75% sterilized alcohol, burning above the flame of alcohol lamp, sterilizing the tweezers, and naturally cooling on sterile roll paper. After 2 days, the paper folding containing pupation was taken out with tweezers and placed in the sterilized glass jar. Meanwhile, 6 paper sheets (13.9 cm long and 10.2cm wide) cut from sterile roll paper are folded into fan-shaped folded paper, placed on the japonica rice seedlings, sealed by sterilized black cloth and fastened by rubber bands to enable the larvae to pupate continuously. Placing in an RGC-1000C artificial climate box (combined fertilizer right department instruments and equipment Co., Ltd.), setting the temperature at 28 ℃, the relative humidity at 80%, and the illumination: dark 16 h: and 8 h. After 2 days, 1 piece of A4 copy paper is laid on the test bed, the folded paper is clamped on the A4 copy paper by tweezers, the folded paper is pulled open by 2 tweezers, pupas in the folded paper are clamped in a 90mm glass culture dish, the bottom of the culture dish is padded by sterile roll paper, the sterile roll paper is wetted by double distilled water, and the development humidity of the pupas is kept.

Example 4 mating and oviposition of Chilo suppressalis adults

(1) Preparation of insect cages

The insect cages (45cm multiplied by 55cm) prepared for mating and oviposition of chilo suppressalis are washed clean by tap water. Then 5L of tap water was poured into a plastic pot with a measuring cup, following sodium hypochlorite solution: and (3) pouring the water which is 1:100 and the required sodium hypochlorite solution with the dosage of 50mL into a plastic basin, and uniformly mixing with tap water to prepare the disinfectant fluid. The ordinary hairbrush is dipped in the disinfectant fluid with the proportion, and the brushing and disinfection are carried out on the insect rearing cage for mating and oviposition of the chilo suppressalis. After the disinfectant fluid is used for washing, the disinfectant fluid is fully washed under tap water until the pungent smell of sodium hypochlorite can not be smelled. And (5) placing the mixture in a normal temperature environment for airing for later use.

(2) Preparation of rice seedlings of japonica rice for egg laying

According to the procedure in example 1, rice shoots of desired japonica rice seedlings for oviposition were prepared. 50g of sterilized rice sprouts are sown in a plastic pot (the caliber is 17.5cm, the height is 15.5cm), scattered on the surface layer of the soil of the pot, covered with a layer of thin soil, placed in an artificial climate chamber, and controlled by an air conditioner at the temperature of 28 ℃, with the light cycle of 16h, 8h of darkness (controlled by a timer), and the relative humidity of the rice sprouts is about 80%. And covering the plastic pot with black plastic bag for heat preservation. After 7 days, rice sprouts in the plastic pot emerge and serve as spawning seedlings, and the plant height of the spawning seedlings is 10 cm.

(3) Adult mating and laying eggs (as shown in figure 4)

The collected pupae were transferred to 90mm glass petri dishes, the bottom of the petri dish was padded with sterile rolled paper, the sterile rolled paper was wetted with double distilled water, and 50 pupae were placed in each petri dish for mating and oviposition. Firstly, placing the disinfected insect cage in an RGC-1000C artificial climate box (combined fertilizer right department instruments and equipment Co., Ltd.), setting the temperature to be 28 ℃, the relative humidity to be 80 percent and the illumination: dark 16 h: and 8 h. Place one the sponge that absorbs water that the size is suitable at the bottom of insect feeding cage, it is moist with the sponge that absorbs water with 100 mL's double distilled water, place 2 pots of rice seedlings that lay eggs in the insect feeding cage with diagonal mode of putting, then place the pupa of 2 boxes of culture dishes in the insect feeding cage.

10mL of pure honey is sucked by a 1000uL liquid-transferring gun and transferred into a disposable cup, 90mL of double distilled water is poured into a measuring cylinder, and an iron medicine spoon is used for fully stirring to prepare 10% of honey water. 20mL of 10% honey water is poured into a culture dish with the diameter of 60mm, and the honey water is dipped in a sterilized cotton ball, so that a feeding carrier is provided for adults, and the adults are prevented from falling into the honey water and dying. Placing 1 culture dish containing Mel solution into insect cage, supplementing nutrition for eclosion adult, promoting its oviposition, changing Mel solution in culture dish every 2 days to ensure Mel water freshness, and preventing mildew and putrefaction. Placing in an artificial climate box at 28 deg.C, allowing pupa to emerge for 4 days, and allowing the emerged imagoes to mate and lay eggs in insect cage. The height of the spawning seedlings is 20 cm.

Example 5 Chilo suppressalis egg piece Collection preservation

And (3) removing the pot of the japonica rice seedling from the artificial climate box after the adult eclosion for 7 days, and removing the adult rice seedling from the rice seedling if the chilo suppressalis adult is still attached to the rice seedling, so that the vitality of the adult rice is weakened and the adult rice is inactive. The eclosion adults will lay eggs on the surface of the rice leaf, and the eggs gather together to form egg masses. Soaking scissors in 75% ethanol, taking out, and wiping with sterile roll paper for sterilization. First, 1 sheet of A4 transfer paper was laid on a test stand, and rice plants having egg clumps were cut from the roots with sterilized scissors and temporarily placed on A4 transfer paper. 1 sterile rolled paper sheet was prepared by laying on the bottom of a 90mm petri dish and wetting the sheet with double distilled water. The rice leaves with the clumps were cut with sterilized scissors and placed in a 90mm petri dish with a wet paper sheet laid on the bottom. 100 japonica rice leaves carrying egg blocks can be cut out from 2 pots of spawning seedlings, and 100-150 egg blocks can be carried.

35mL of formaldehyde is absorbed by a 5mL pipette and transferred to a disposable cup, 65mL of double distilled water is poured into a measuring cylinder, and the mixture is fully stirred to prepare 35% formaldehyde solution. The culture dish with the egg mass is placed in a glass drying dish with formaldehyde solution at the bottom for fumigation for 15min, and is taken out immediately after the fumigation is finished, so that the egg mass is disinfected through fumigation, and parasitic bacteria on the surface of the egg mass are killed (as shown in figure 5).

Then, placing the egg blocks in an artificial climate box at the temperature of 28 ℃, and carrying out cultivation and feeding in the next period after 0-2 days; or, the egg blocks are refrigerated at the low temperature of 4 ℃ in a refrigerator, so that the development progress of the eggs is kept consistent, and the incubation time of the egg blocks is controlled.

In addition to the above embodiments, the present invention may have other embodiments. All technical solutions formed by adopting equivalent substitutions or equivalent transformations fall within the protection scope of the claims of the present invention.

Claims

1. An accurate large-scale feeding method for indoor rice seedlings of chilo suppressalis is characterized by comprising the following steps:

2. The accurate large-scale breeding method of chilo suppressalis indoor rice seedlings according to claim 1, wherein the can bottle containing the larvae in the third step, the culture dish containing the pupae in the fourth step or the egg mass obtained in the sixth step are placed in an environment with the temperature of 4 +/-2 ℃ for a preset time to control the development progress of the eggs.

3. The accurate large-scale feeding method of Chilo suppressalis indoor rice seedlings according to claim 1 or 2, characterized in that in the first step, the specific processes of cleaning and elutriating rice seeds are as follows:

4. The accurate large-scale feeding method of Chilo suppressalis indoor rice seedlings according to claim 3, is characterized in that in the second step, the specific process of accelerating germination to obtain rice sprouts is as follows:

5. The accurate large-scale feeding method of Chilo suppressalis indoor rice seedlings according to claim 4, is characterized in that in the second step, the specific process of accelerating the seedlings to obtain the rice seedlings is as follows:

6. The accurate large-scale feeding method of Chilo suppressalis indoor rice seedlings according to claim 5, characterized in that in the third step, tweezers disinfected by medical alcohol are adopted, burned by an alcohol lamp and then naturally cooled for standby; when transferring the larvae from the current can bottle to the rice seedlings in a new can bottle, clamping the rice seedlings from the current can bottle by using tweezers and placing the rice seedlings on a first piece of clean paper, picking out the larvae from the rice seedlings and placing the larvae on a second piece of clean paper, and finally directly pouring the larvae on the second piece of clean paper onto the rice seedlings in the new can bottle, and if the larvae escape from the clean paper, picking the larvae onto the rice seedlings in the new can bottle by using the tweezers; then, sealing and fastening the can bottle by using sterilized black cloth, and continuously placing the can bottle in an artificial climate box with the temperature of 28 +/-1 ℃ and the relative humidity of 70-80%; in the third step, the larvae are kept in a climatic chamber where the environment is kept constant throughout the rearing process.

7. The accurate large-scale feeding method of Chilo suppressalis indoor rice seedlings according to claim 5, characterized in that in the fourth step, the folded paper is put into a sterilized glass jar when each folded paper is taken out, and then the subsequent operation is carried out; the sterilized instrument is medical forceps sterilized by medical alcohol; the caliber of the culture dish is 90 mm; in the fifth step, the caliber of the culture dish is 90 mm; the sponge was wetted with 100 + -10 mL double distilled water.

8. The accurate large-scale feeding method of Chilo suppressalis indoor rice seedlings according to claim 5, is characterized in that in the fifth step, the process of breeding the rice seedlings for oviposition is as follows: taking rice sprouts obtained by germination accelerating in the second step, sowing the rice sprouts on the surface layer of soil of a preset pot and covering a layer of thin soil, covering the pot with a clean black plastic bag, and culturing in an artificial climate chamber with the temperature of 28 +/-1 ℃ and the relative humidity of 70-80% until the plant height of rice seedlings reaches 10cm, thus obtaining the rice seedlings for spawning; fifthly, a culture dish with the caliber of 60mm is also placed in the insect breeding cage, and the culture dish is filled with sterilized cotton balls dipped with honey water with the volume ratio of 10 +/-1%; the cotton ball was replaced every two days.

9. The accurate large-scale feeding method of Chilo suppressalis indoor rice seedlings according to claim 5, is characterized in that in the sixth step, the specific fumigation process is as follows: putting the culture dish containing the egg mass into a drying cylinder with formaldehyde solution at the bottom for fumigating for 15 +/-1 min, and then immediately taking out; the sterilized instrument is sterilized scissors; the caliber of the culture dish is 90 mm; the concentration of the formaldehyde solution is 35 +/-1 percent by volume.

10. The accurate large-scale feeding method of Chilo suppressalis indoor rice seedlings according to claim 5, is characterized in that the rice seeds are japonica rice seeds; the caliber of the can bottle is 6cm and the height of the can bottle is 10 cm; the insect cage has the following dimensions: 45cm × 45cm × 55 cm; the number of the cans adopted in the third step is more than 20.