CN103960204B - A kind of method of raising lepidopterous insects - Google Patents
A kind of method of raising lepidopterous insects Download PDFInfo
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- CN103960204B CN103960204B CN201410234275.1A CN201410234275A CN103960204B CN 103960204 B CN103960204 B CN 103960204B CN 201410234275 A CN201410234275 A CN 201410234275A CN 103960204 B CN103960204 B CN 103960204B
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Abstract
The invention discloses a kind of method of raising lepidopterous insects, using pellet type cylinder mould as mainly raising instrument in feeding process, comprising the step such as ovum collecting and sterilization, egg hatching larva and larva raising, pupa raising and imago breeding.Collect ovum using gauze or plastic ties as oviposition material, with the formalin of volumetric concentration 3-4%, ovum is sterilized; Egg hatching and raising is carried out in illumination box; Sterilize to pupa with volumetric concentration 8-12% liquor natrii hypochloritis, pupa is sprouted wings in pellet type cylinder mould, and the adult after emergence continues to raise in cylinder mould, carries out ovum collection and sterilization.Pellet type cylinder mould of the present invention, easy and simple to handle, both can effectively prevent insect escape, and also be conducive to carrying out cleaning at any time; In feeding process, ovum and pupa adopt different disinfectants, and sterilization is thoroughly gentle, both effective anti-bacteria amount reproduction, and also can improve egg hatching rate and pupa eclosion rate, the incubation rate of ovum can reach 95-96%, and pupa eclosion rate can reach 98%.
Description
Technical field
The present invention relates to the test method that lepidoptera imago emergence, ovum collecting, larva and pupa are raised, be specifically related to a kind of method of raising lepidopterous insects.
Background technology
Lepidoptera is the second largest order of Insecta, and the lepidopterous insects such as silkworm, tussah, eri silkworm are very important economic insects, the Chinese herbal medicine that the Cordyceps sinensis that bat moth larvae is formed by fungi autoeciousness is famous and precious especially; On the other hand, cotton bollworm, mythimna separata, beet armyworm etc. are important pest, cause tremendous economic to lose every year.
Lepidopterous insects completes a generation needs experience ovum, larva, pupa and four periods of adult, and larva uses artificial diet usually, and technology is relative maturity; And adult must provide larger cultured space to make male and female post-coitum ability egg reproduction offspring, current raising device mostly is square crib or rearing-box, the feeding hamper of adult and oviposition material is placed in inside and raises.
Above-mentioned crib or rearing-box need often to open cage door when changing food or gather worm's ovum in feeding process, not only complex operation and easily cause adult wait for an opportunity escape, in laboratory of being everlasting, spend manpower to catch the adult escaped, work consuming consuming time, and feeding efficiency is lower.And traditional raising method is difficult to control to raising the degree of sterilizing in the disinfecting process of crib or rearing-box, sterilization is usually caused thoroughly not cause bacterium, viral amount reproduction, thus make the bacterial infection such as larva, pupa, virus, therefore the defect in above-mentioned disinfecting process usually causes egg hatching rate, larvae pupation rate, pupa eclosion rate and adult survival rate lower, is unfavorable for experimental study and practical application.
Summary of the invention
Technical problem to be solved by this invention is the defect existed for prior art, and a kind of method of raising lepidopterous insects is provided, and utilize pellet type cylinder mould as raising instrument, easy and simple to handle, can effectively prevent insect escape, be conducive to carrying out cleaning at any time simultaneously; In feeding process, take accurate disinfectant measure, effective anti-bacteria amount reproduction, improve egg hatching rate and pupa eclosion rate.
In order to achieve the above object, the present invention is achieved in that
Raise a method for lepidopterous insects, comprise the following steps:
(1) ovum collecting and sterilization: the 2-3d after the adult eclosion in pellet type cylinder mould, namely, time preoviposition period that adult being in, oviposition material is hung on cage top or enclose in cage peripheral, the 3-6d after adult eclosion collects the oviposition material with ovum, later every day collects once, collects 3-9d altogether; After ovum collecting, be the formalin sterilization 10-16min of 3%-4% the oviposition material volumetric concentration with ovum, then take out to be placed in sterile water with aseptic nipper and soak 3 times, each 20-30min; Immersion terminates rear taking-up and is put in Indoor Natural and dries.
(2) egg hatching larva and larva raising: spread layer 2-3 blotting paper bottom aseptic raising bottle, then the oviposition material after drying in step 1 is placed in aseptic raising bottle, be placed in illumination box after airtight and cultivate, condition of culture is temperature 22-28 DEG C, photoperiod L:D=14h:10h; Cultivate egg hatching after 3-6d and become larva, after larvae hatch, open the lid of raising bottle, then putting into that 4-8 block amasss is 1 cm
3larva artificial feed, make larva be gathered on artificial feed; Raise in illumination box, rearing conditions is temperature 22-28 DEG C, photoperiod L:D=14h:10h, regularly replaces new artificial feed, and after raising 10-22d, larva becomes pupa.
(3) raising of pupa: the pupa in step 2 is collected together by the 2-3d after pupating, the volumetric concentration putting into 250ml in sterile beaker is 8-12% liquor natrii hypochloritis; Then pupa is immersed in liquor natrii hypochloritis and carries out disinfection, after sterilization 4-6min, with aseptic water washing 3 times, be placed on blotting paper and naturally dry; In the emergence box of pellet type cylinder mould, add the sterile vermiculite of box 1/2nd volume, putting into the pupa dried after regulating vermiculite humidity, is the sterile vermiculite of 0.5-1cm at pupa upper berth lid layer thickness; Then cover on emergence box by the masking foil being equipped with 4-5 hole, the size in hole climbs out of the best with applicable adult; Be placed on by cage in the thermostatic chamber of 22-28 DEG C and raise, after raising 5-11d, pupa sprouts wings into adult.
(4) imago breeding and laying eggs: after having observed adult eclosion, add the hydromel that mass concentration is 10% in the feeding hamper of pellet type cylinder mould, by feeding hamper is inverted in cylinder mould top after covering feeding hamper with sterile gauze, and adult sucks hydromel and supplements the nutrients; Change a hydromel every day; Time when imago breeding is in preoviposition period (2-3d after adult eclosion), oviposition material is hung on cage top or enclose in cage peripheral, collection and the sterilization of ovum is carried out in the operating process repeating step 1, completes the cultivation of a generation; The life-span of adult is generally 8-14d, after adult death, is cleaned up by cylinder mould in order to using again.
Preferably, above-mentioned aseptic nipper, sterile water, aseptic raising bottle, sterile beaker, sterile vermiculite and sterile gauze, prepare after being autoclave sterilization 20min, use after being cooled to room temperature again.
Preferably, the concentration of the formalin in step 1 is 3.7%, and disinfecting time is 15min.
Preferably, the concentration of the clorox in step 3 is 10%, and disinfecting time is 5min.
Preferably, the adjustment vermiculite humidity described in step 3 adds sterile water in vermiculite, but vermiculite is adjusted to moistening do not drip till.
The present invention also provides the cylinder mould of the pellet type described in a kind of said method, and comprise pallet, emergence box, cylinder mould and feeding hamper, it is characterized in that, cylinder mould is fixed on pallet with embedded mode, and emergence box is put on pallet as the emergence place of pupa; Described emergence box is rectangular box, and the vermiculite being used for covering pupa is placed in box inside, and box top is surrounded by masking foil, and masking foil has the aperture climbed out of for adult; Described feeding hamper is cylindrical box body, and box is built with hydromel, and box mouth sterile gauze seals, and uses rubber band tying, is inverted in the top of cylinder mould; Oviposition material is placed at the periphery of cylinder mould or top.
Preferably, above-mentioned oviposition material is gauze or plastic ties, and bundle is trapped among cylinder mould periphery for cotton boll insects Adult worms producting eggs, and plastic ties hangs on cylinder mould top for mythimna separata class Adult worms producting eggs.
Preferably, described emergence box, feeding hamper material are plastics system, and the specification of emergence box is long 13.5cm, wide 8.5cm, high 4.5cm; Described pallet material is sheet iron, and shape is cylindrical, and the diameter of pallet is 24.0cm, high 4.0cm.
Preferably, described cylinder mould comprises stainless (steel) wire and circular steel ring, and the stainless (steel) wire mesh length of side is 0.5-0.8cm, and mesh size is according to the size adjustment of raising lepidoptera imago, and the internal diameter of circular steel ring is 23.0cm, and steel ring is that the steel wire that 0.4cm is thick is made by diameter; The surrounding of cylinder mould is welded in inside circular steel ring by stainless (steel) wire, and the top of cylinder mould is the downside that stainless (steel) wire is welded in circular steel ring, and cylinder mould top steel ring and side steel ring are welded together to form cylinder mould.
The invention has the beneficial effects as follows: using pellet type cylinder mould as mainly raising instrument, easy and simple to handle, both can effectively prevent insect escape, be also conducive to carrying out cleaning-sterilizing work at any time; In feeding process, ovum and pupa adopt different disinfectants respectively, under rational concentration and disinfecting time combine, sterilization is thorough and process is gentle, both effective anti-bacteria amount reproduction, can also improve egg hatching rate and pupa eclosion rate, the incubation rate of its ovum can reach 95-96%, and the eclosion rate of pupa can reach 98%.
Accompanying drawing explanation
Fig. 1: the overall structure schematic diagram of pellet type cylinder mould of the present invention;
Wherein: the aperture 1, on pallet 2, emergence box 3, cylinder mould 4, feeding hamper 5, masking foil.
Embodiment
Below in conjunction with embodiment, raising method of the present invention is described in further detail.
Raise a method for lepidopterous insects, comprise the following steps:
(1) ovum collecting and sterilization: the 2-3d after adult eclosion, oviposition material is hung on cage top or enclose in cage peripheral, the 3-6d after adult eclosion collects ovum from oviposition material, and later every day collects once, collects 3-9d altogether; After ovum collecting, with formalin sterilization, then soak with in sterile water; Immersion terminates rear taking-up and naturally dries.
(2) egg hatching larva and larva raising: spread blotting paper bottom raising bottle, is placed on the oviposition material after drying in raising bottle, is placed in illumination box and cultivates after airtight; After cultivating 3-6d, egg hatching becomes larva, and after larvae hatch, open the lid of raising box, continue to raise at illumination box, regularly replace new artificial feed after putting into larva artificial feed, after raising 10-22d, larva becomes pupa.
(3) raising of pupa: pupa is collected together by the 2-3d after pupating, and carries out disinfection with liquor natrii hypochloritis, rear aseptic water washing of sterilizing, is placed on blotting paper and dries; In emergence box, add the sterile vermiculite of 1/2nd volumes, after regulating vermiculite humidity, put into the pupa dried, bedding sterile vermiculite on pupa; Then cover on emergence box by the masking foil being equipped with 4-5 hole, after raising 5-11d, pupa sprouts wings into adult.
(4) imago breeding and laying eggs: after having adult eclosion, add hydromel in feeding hamper, by feeding hamper is inverted in cylinder mould top after covering feeding hamper with sterile gauze; Change a hydromel every day; As the 2-3d after adult eclosion, oviposition material is hung on cage top or enclose in cage peripheral, collection and the sterilization of ovum is carried out in the operating process repeating step 1, completes the raising of a generation.
Below in conjunction with accompanying drawing, pellet type cylinder mould of the present invention is described:
As shown in Figure 1, a kind of pellet type cylinder mould, comprises pallet 1, emergence box 2, cylinder mould 3 and feeding hamper 4, and cylinder mould 3 is fixed on pallet 1 with embedded mode, and emergence box 2 is put on pallet 1; Emergence box 2 is plastic rectangular box, and the vermiculite being used for covering pupa is placed in box inside, and specification the best of emergence box 2 is long 13.5cm, wide 8.5cm, high 4.5cm; Emergence box 2 top is surrounded by masking foil, and masking foil has the aperture 5 climbed out of for adult; Feeding hamper 4 is plastic cylindrical box body, and box is built with hydromel, and box mouth sterile gauze seals, and uses rubber band tying, is inverted in the top of cylinder mould 3; Oviposition material is placed at the periphery of cylinder mould 3 or top, and oviposition material is gauze or plastic ties, and bundle is trapped among cylinder mould periphery for cotton boll insects Adult worms producting eggs, and plastic ties hangs on cylinder mould top for mythimna separata class Adult worms producting eggs.The material of pallet 1 is preferably sheet iron, and shape is cylindrical, and the diameter of pallet 1 is 24.0cm, high 4.0cm.Cylinder mould 3 comprises stainless (steel) wire and circular steel ring, and the stainless (steel) wire mesh length of side is 0.5-0.8cm, and mesh size is according to the size adjustment of raising lepidoptera imago, and the internal diameter of circular steel ring is 23.0cm, and steel ring is that the steel wire that 0.4cm is thick is made by diameter; The surrounding of cylinder mould 3 is welded in inside circular steel ring by stainless (steel) wire, and the top of cylinder mould 3 is the downside that stainless (steel) wire is welded in circular steel ring, and cylinder mould top steel ring and side steel ring are welded together to form cylinder mould.
Below in conjunction with embodiment, raising method of the present invention is described in further detail.
Embodiment 1:
The raising of mythimna separata:
(1) ovum collecting and sterilization: the 2-3d after the adult eclosion in pellet type cylinder mould, hangs on cage top by plastic ties, the 5d after adult eclosion collects ovum from plastic ties, and later every day collects once, collects 8-9d altogether; After ovum collecting, be the formalin sterilization 15min of 3.7% the plastic ties volumetric concentration with ovum, then take out to be placed in sterile water with aseptic nipper and soak 3 times, each 30min; Immersion terminates rear taking-up and is put in Indoor Natural and dries;
(2) egg hatching larva and larva raising: spread layer 2-3 blotting paper bottom aseptic raising bottle, then the plastic ties after drying in step 1 is placed in aseptic raising bottle, be placed in illumination box after airtight and cultivate, condition of culture is temperature 22 DEG C, photoperiod L:D=14h:10h; Cultivate egg hatching after 5d and become larva, after larvae hatch, open the lid of raising bottle, then putting into that 4-5 block amasss is 1 cm
3larva artificial feed, raise in illumination box, rearing conditions is temperature 22 DEG C, photoperiod L:D=14h:10h, regularly replaces new artificial feed, raises larva after 18-22d and becomes pupa;
(3) raising of pupa: the pupa in step 2 is collected together by the 2-3d after pupating, the volumetric concentration putting into 250ml in sterile beaker is 10% liquor natrii hypochloritis; Then pupa is immersed in liquor natrii hypochloritis and carries out disinfection, after sterilization 5min, with aseptic water washing 3 times, be placed on blotting paper and naturally dry; In the emergence box of pellet type cylinder mould, add the sterile vermiculite of box 1/2nd volume, put into the pupa dried after regulating vermiculite humidity, 50 pupas placed by every box, are the sterile vermiculite of 0.5-1cm at pupa upper berth lid layer thickness; Then cover on emergence box by the masking foil being equipped with 4-5 hole, the size in hole climbs out of the best with applicable mythimna separata adult; Be placed on by cage in the thermostatic chamber of 28 DEG C and raise, after raising 8-9d, pupa sprouts wings into adult;
(4) imago breeding and laying eggs: when having observed after adult occurs, having added the hydromel that mass concentration is 10% in the feeding hamper of pellet type cylinder mould, after covering feeding hamper with sterile gauze, feeding hamper being inverted in cylinder mould top, changing a hydromel every day; 2-3d after adult eclosion, hangs on cage top by plastic ties, collection and the sterilization of ovum is carried out in the operating process repeating step 1, completes the raising of a generation;
In step 2, the incubation rate of statistics egg hatching larva, cut the plastic ties 10 that 1-2cm long band has ovum, the ovum quantity in every bar plastic ties is added up under binocular stereomicroscope, be placed on respectively in aseptic raising box and hatch, add up the quantity of larva in every box, computing formula is as follows: incubation rate=larva quantity/ovum quantity (× 100%), and after cultivation 5d, average percentage hatch rate is 96%.
In step 3, add up the eclosion rate of every box pupa, computing formula is as follows: eclosion rate=adult quantity/pupa quantity (× 100%), and after cultivating 9d, average eclosion rate is 98%.
Embodiment 2:
The raising of beet armyworm:
(1) ovum collecting and sterilization: the 2d after the adult eclosion in pellet type cylinder mould, hangs on cage top by plastic ties, the 3d after adult eclosion collects ovum from plastic ties, and later every day collects once, collects 3-5d altogether; After ovum collecting, be the formalin sterilization 10min of 3.7% the plastic ties volumetric concentration with ovum, then take out to be placed in sterile water with aseptic nipper and soak 3 times, each 20min; Immersion terminates rear taking-up and puts into Indoor Natural and dry;
(2) egg hatching larva and larva raising: spread layer 2-3 blotting paper bottom aseptic raising bottle, then the plastic ties after drying in step 1 is placed in aseptic raising bottle, be placed in illumination box after airtight and cultivate, condition of culture is temperature 28 DEG C, photoperiod L:D=14h:10h; Cultivate egg hatching after 3d and become larva, after larvae hatch, open the lid of raising bottle, then putting into that 4-5 block amasss is 1 cm
3larva artificial feed, raise in illumination box, rearing conditions is temperature 28 DEG C, photoperiod L:D=14h:10h, regularly replaces new artificial feed, raises larva after 9-10d and becomes pupa;
(3) raising of pupa: the pupa in step 2 is collected together by the 2-3d after pupating, the volumetric concentration putting into 250ml in sterile beaker is 10% liquor natrii hypochloritis; Then pupa is immersed in liquor natrii hypochloritis and carries out disinfection, after sterilization 5min, with aseptic water washing 3 times, be placed on blotting paper and naturally dry; In the emergence box of pellet type cylinder mould, add the sterile vermiculite of box 1/2nd volume, put into the pupa dried after regulating vermiculite humidity, 50 pupas placed by every box, are the sterile vermiculite of 0.5-1cm at pupa upper berth lid layer thickness; Then cover on emergence box by the masking foil being equipped with 4-5 hole, the size in hole climbs out of the best with applicable beet armyworm adult; Be placed on by cage in the thermostatic chamber of 28 DEG C and raise, after raising 5d, pupa sprouts wings into adult;
(4) imago breeding and laying eggs: when having observed after adult occurs, having added the hydromel that mass concentration is 10% in the feeding hamper of pellet type cylinder mould, after covering feeding hamper with sterile gauze, feeding hamper being inverted in cylinder mould top, changing a hydromel every day; 2-3d after adult eclosion, hangs on cage top by plastic ties, collection and the sterilization of ovum is carried out in the operating process repeating step 1, completes the cultivation of a generation;
In step 2, the incubation rate of statistics egg hatching larva, cut the plastic ties 10 that 1-2cm long band has ovum, the ovum quantity in every bar plastic ties is added up under binocular stereomicroscope, be placed on respectively in aseptic raising box and hatch, add up the quantity of larva in every box, computing formula is as follows: incubation rate=larva quantity/ovum quantity (× 100%), and after cultivation 3d, average percentage hatch rate is 96%.
In step 3, add up the eclosion rate of every box pupa, computing formula is as follows: eclosion rate=adult quantity/pupa quantity (× 100%), and after cultivating 5d, average eclosion rate is 98%.
Embodiment 3:
The raising of cotton bollworm:
(1) ovum collecting and sterilization: the 2-3d after the adult eclosion in pellet type cylinder mould, is trapped among gauze around cylinder mould, the 4d after adult eclosion collects ovum from gauze, and later every day collects once, collects 5-8d altogether; After ovum collecting, be the formalin sterilization 10min of 3.7% the gauze volumetric concentration with ovum, then take out to be placed in sterile water with aseptic nipper and soak 3 times, each 20min; Immersion terminates rear taking-up and is put in Indoor Natural and dries;
(2) egg hatching larva and larva raising: spread layer 2-3 blotting paper bottom aseptic raising bottle, then the gauze after drying in step 1 is placed in aseptic raising bottle, be placed in illumination box after airtight and cultivate, condition of culture is temperature 25 DEG C, photoperiod L:D=14h:10h, and after cultivating 3-4d, egg hatching becomes larva; After larvae hatch, open the lid of raising bottle, put into 4-5 block and amass as 1cm
3artificial feed raise; Because cotton bollworm has internecine habit, larva 3 carries out single box raising after age, and it is 1 cm that each spool box puts into that 1-2 block amasss
3larva artificial feed, each spool box is raised after putting a larva in illumination box, and rearing conditions is temperature 25 DEG C, photoperiod L:D=14h:10h, raises larva after 10-11d and becomes pupa;
(3) raising of pupa: the pupa in step 2 is collected together by the 2-3d after pupating, the volumetric concentration putting into 250ml in sterile beaker is 10% liquor natrii hypochloritis; Then pupa is immersed in liquor natrii hypochloritis and carries out disinfection, after sterilization 5min, with aseptic water washing 3 times, be placed on blotting paper and naturally dry; In the emergence box of pellet type cylinder mould, add the sterile vermiculite of 1/2nd volumes, put into the pupa dried after regulating vermiculite humidity, 50 pupas placed by every box, are the sterile vermiculite of 0.5-1cm at pupa upper berth lid layer thickness; Then cover on emergence box by the masking foil being equipped with 4-5 hole, the size in hole climbs out of the best with applicable cotton bollworm adult; Be placed on by cage in the thermostatic chamber of 28 DEG C and raise, after raising 10-11d, pupa sprouts wings into adult;
(4) imago breeding and laying eggs: when having observed after adult occurs, having added the hydromel that mass concentration is 10% in the feeding hamper of pellet type cylinder mould, after covering feeding hamper with sterile gauze, feeding hamper being inverted in cylinder mould top, changing a hydromel every day; 2-3d after adult eclosion, is trapped among gauze around cylinder mould, and collection and the sterilization of ovum is carried out in the operating process repeating step 1, completes the cultivation of a generation;
In step 2, the incubation rate of statistics egg hatching larva, cuts 1-2cm
210 pieces, gauze, add up the ovum quantity on every block gauze under binocular stereomicroscope, be placed on respectively in aseptic raising bottle and hatch, the quantity of larva in adding up every bottle, computing formula is as follows: incubation rate=larva quantity/ovum quantity (× 100%), and after cultivation 4d, average percentage hatch rate is 95%.
In step 3, add up the eclosion rate of every box pupa, computing formula is as follows: eclosion rate=adult quantity/pupa quantity (× 100%), and after cultivating 11d, average eclosion rate is 98%.
The present invention also comprises the change fallen in the scope of the invention, displacement and various substitute equivalents.Here the example provided is only illustrative, instead of limitation of the present invention.
Claims (9)
1. raise a method for lepidopterous insects, it is characterized in that, comprise the steps:
(1) ovum collecting and sterilization: the 2-3d after the adult eclosion in pellet type cylinder mould, oviposition material is hung on cylinder mould top or enclose in cylinder mould peripheral, within the 3rd day after adult eclosion, the 4th day, the 5th day or the 6th day, collect the oviposition material with ovum, later every day collects once, collects 3-9d altogether; After ovum collecting, be the formalin sterilization 10-16min of 3%-4% the oviposition material volumetric concentration with ovum, then take out to be placed in sterile water with aseptic nipper and soak 3 times, each 20-30min; Immersion terminates rear taking-up and is put in Indoor Natural and dries;
(2) egg hatching larva and larva raising: spread layer 2-3 blotting paper bottom aseptic raising box, then the oviposition material after drying in step (1) is placed in aseptic raising box, be placed in illumination box after airtight and cultivate, condition of culture is temperature 22-28 DEG C, photoperiod L ︰ D=14h ︰ 10h; After cultivation 3-6d, egg hatching is larva, after larvae hatch, opens the lid of raising box, puts into 4-8 block and amass as 1cm
3larva artificial feed, raise in illumination box, rearing conditions is temperature 22-28 DEG C, photoperiod L ︰ D=14h ︰ 10h, regularly replaces new artificial feed, raises larva after 10-22d and becomes pupa;
(3) raising of pupa: the pupa in step (2) is collected together by the 2-3d after pupating, the volumetric concentration putting into 250ml in sterile beaker is 8-12% liquor natrii hypochloritis; Pupa is immersed in liquor natrii hypochloritis and carries out disinfection, after sterilization 4-6min, with aseptic water washing 3 times, be placed on blotting paper and naturally dry; In the emergence box of pellet type cylinder mould, add the sterile vermiculite of 1/2nd volumes, putting into the pupa dried after regulating vermiculite humidity, is the sterile vermiculite of 0.5-1cm at pupa upper berth lid layer thickness; Then the masking foil being equipped with 4-5 hole is covered on emergence box; Be placed on by cylinder mould in the thermostatic chamber of 22-28 DEG C and raise, after raising 5 ~ 11d, pupa sprouts wings into adult;
(4) imago breeding and laying eggs: after adult eclosion, add the hydromel that mass concentration is 10% in the feeding hamper of pellet type cylinder mould, is inverted in cylinder mould top by feeding hamper after covering feeding hamper, changes a hydromel every day with sterile gauze; As the 2-3d after adult eclosion, oviposition material is hung on cylinder mould top or enclose in cylinder mould peripheral, collection and the sterilization of ovum is carried out in the operating process repeating step (1), completes the cultivation of a generation.
2. method according to claim 1, is characterized in that, described aseptic nipper, sterile water, aseptic raising box, sterile beaker, sterile vermiculite and sterile gauze, prepare after being autoclave sterilization 20min, use after being cooled to room temperature again.
3. method according to claim 1, is characterized in that, the volumetric concentration of the formalin described in step (1) is 3.7%, and disinfecting time is 15min.
4. method according to claim 1, is characterized in that, the volumetric concentration of the clorox described in step (3) is 10%, and disinfecting time is 5min.
5. method according to claim 1, is characterized in that, the adjustment vermiculite humidity described in step (3) adds sterile water in vermiculite, but is adjusted to by vermiculite and does not moisteningly drip.
6. the pellet type cylinder mould described in a claim 1, comprise pallet (1), emergence box (2), cylinder mould (3) and feeding hamper (4), it is characterized in that, cylinder mould is fixed on pallet with embedded mode, and emergence box is put on pallet as the emergence place of pupa; Described emergence box is rectangular box, and the vermiculite being used for covering pupa is placed in box inside, and box top is surrounded by masking foil, and masking foil has the aperture (5) climbed out of for adult; Described feeding hamper is cylindrical box body, and box is built with hydromel, and box mouth sterile gauze seals, and uses rubber band tying, is inverted in the top of cylinder mould; Oviposition material is placed at the periphery of cylinder mould or top.
7. pellet type cylinder mould according to claim 6, is characterized in that, described oviposition material is gauze or plastic ties, and bundle is trapped among cylinder mould periphery for cotton boll insects Adult worms producting eggs, and plastic ties hangs on cylinder mould top for mythimna separata class Adult worms producting eggs.
8. pellet type cylinder mould according to claim 6, is characterized in that, described emergence box, the material of feeding hamper are plastics system, and the specification of emergence box is long 13.5cm, wide 8.5cm, high 4.5cm; Described pallet material is sheet iron, and shape is cylindrical, and the diameter of pallet is 24.0cm, high 4.0cm.
9. pellet type cylinder mould according to claim 6, it is characterized in that, described cylinder mould comprises stainless (steel) wire and circular steel ring, the stainless (steel) wire mesh length of side is 0.5-0.8cm, mesh size is according to the size adjustment of raising lepidoptera imago, the internal diameter of circular steel ring is 23.0cm, and steel ring is that the steel wire that 0.4cm is thick is made by diameter; The surrounding of cylinder mould is welded in inside circular steel ring by stainless (steel) wire, and the top of cylinder mould is the downside that stainless (steel) wire is welded in circular steel ring, and cylinder mould top steel ring and side steel ring are welded together to form cylinder mould.
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|---|---|---|---|---|
| CN104756959A (en) * | 2015-04-24 | 2015-07-08 | 新疆农业科学院植物保护研究所 | Oviposition cage for small and medium-sized lepidoptera moth insects |
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| JP2008054687A (en) * | 2007-10-05 | 2008-03-13 | Tsuwano Machi | Plant worm, plant worm tea, and food and drink |
| CN101642079B (en) * | 2009-08-27 | 2012-05-30 | 广东省农业科学院作物研究所 | Classified raising method and imago raising device of corn borers |
| CN201624071U (en) * | 2010-01-29 | 2010-11-10 | 华南农业大学 | Raising device for chrysopa perla imagoes |
| CN102578425B (en) * | 2012-03-14 | 2014-02-19 | 中国农业科学院植物保护研究所 | Artificial diet for Lepidopterous young larvae, preparation method for artificial diet and application of artificial diet |
| CN102599116B (en) * | 2012-04-09 | 2013-11-13 | 天津师范大学 | Method for artificial feeding of Zeuzera leuconolum Butler |
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