CN101642079B - Classified raising method and imago raising device of corn borers - Google Patents
Classified raising method and imago raising device of corn borers Download PDFInfo
- Publication number
- CN101642079B CN101642079B CN2009100421921A CN200910042192A CN101642079B CN 101642079 B CN101642079 B CN 101642079B CN 2009100421921 A CN2009100421921 A CN 2009100421921A CN 200910042192 A CN200910042192 A CN 200910042192A CN 101642079 B CN101642079 B CN 101642079B
- Authority
- CN
- China
- Prior art keywords
- bottle
- raising
- corn borer
- imago
- larvae
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Images
Abstract
The invention provides a classified raising method and an imago raising device of corn borers. The raising method comprises the following steps: raising initial larvae of corn borers in a 12-bore cell culture plate; after 6-7 days, selecting the strong larvae growing regularly to be raised in a tissue culture bottle; after the larvae grow to old larvae, adding paper pupation device into the tissue culture bottle; and finally selecting strong pupae to be put in an imago cage. The imago raising device of corn borers comprises an inner bottle and an outer cover, wherein the inner bottle is an open-top bottle, the lower part of the bottle is provided with a hole in which a hollow area is arranged, the hollow area is provided with a fine-bore screen, the outer cover is sleeved outside the inner bottle, and the top end of the opening of the outer cover is covered with a white gauze; the opening of the inner bottle is provided with an egg laying substrate, and sterilizing sand is arranged on the bottom; and the fine-bore screen is also provided with a food feeding pipe. The method can remove sick and weak corn borers and keep the corn borer strong and regular. The device has the advantages of simple structure, low cost, simplicity and practicability, favorable ventilation property and air humility and wide application range.
Description
Technical field
The present invention relates to raising method and the device thereof of insect, be specifically related to breed in grades method and the imago breeding device thereof of a kind of corn borer.
Background technology
The raising method of conventional corn borer is use bulk container, such as glass jar as raising container, add artificial feed after, be inverted in the ovum paper that is about to hatching on the feed block, larva hatch the back grow with regard to stay in such container always, until pupating.The greatest drawback of this method be larva through screening, support to 3-4 and begin to occur tangible individual difference after age, whole raising population is uneven, it is irregular to cause pupating, and drags chronicly, has a strong impact on feeding efficiency; And indivedual sick worms also infect whole colony easily, cause sick and weak individual increasing, and lopsided pupa increases, thereby are difficult to guarantee the stalwartness of whole raising colony.In a word, larval stage not only influences contemporary feeding efficiency without eliminating choosing, also can influence continuous subculture feeding effect.
Mostly the corn borer imago breeding device of routine is the square cage of wooden rib, and this type of device inconvenience is provided with the substrate of laying eggs, and changes every day and operates also very trouble when laying eggs substrate, and cause adult to wait for an opportunity to escape easily; The moisture-keeping functions of this type of dependent insect cage is not strong yet, is mostly in cage to place a cuvette clear water and preserves moisture, and so not only the water evaporates face is limited, and cause easily the adult mistake fall over into water in and dead.In addition, this type of dependent insect cage is not owing to be provided with outer cover, and moth in batch is movable in cage can be produced a large amount of scales and fly upward everywhere, not only is unfavorable for the sanitation and hygiene of feeding environment, also causes the passive suction of scale keeper's respiratory tract easily and unhealthful.
Summary of the invention
For overcoming above-mentioned technological deficiency, the purpose of this invention is to provide breed in grades method and the imago breeding device thereof of a kind of corn borer.
The breed in grades method of corn borer of the present invention is the corn borer newly hatched larvae to be positioned in a kind of container raise, and after 6-7 days, selects neat, the healthy and strong corn borer larvae of growth and changes in another container and raise.
Preferably, said method specifically comprises the steps:
(1) the corn borer newly hatched larvae is positioned in the 12 porocyte culture plates raises;
(2) after 6-7 days, select neat, the healthy and strong corn borer larvae of growth and change in the tissue culture flasks and raise;
When (3) treating that larva grew to for 5 ages, add the papery device of pupating in the tissue culture flasks in said step (2), be beneficial to mature larva and pierce and pupate;
(4) wait to pupate after, strip and select healthy and strong pupa and go into imago cage.
Preferably, put into 5-10 newly hatched larvae in every hole of the Tissue Culture Plate in the said step (1).
Preferably, in the tissue culture flasks in the said step (2) every bottle put into the 20-30 head through eliminating the larva of choosing.
Papery in the said step (3) device of pupating is a paper cylinder, and said cylinder has the groove wrinkle.
Two of the object of the invention is the raising device that propose a kind of corn borer adult, and bottle and outer cover in this device comprises, interior bottle are uncovered bottle; Aperture is arranged at the body bottom; There is vacancy section at the middle part, and vacancy section is provided with fine-meshed screen, and outer cover is placed in outside the said interior bottle; The top that outer cover is uncovered covers white gauze, and the diameter of outer cover is 1.5-2 a times of interior bottle.
Preferably, said fine-meshed screen comprises gauze screen or nylon wire, and said fine-meshed screen is a 10-20 order net.
Preferably, the bottle mouth position of said interior bottle has the substrate of laying eggs, and the said substrate of laying eggs comprises paraffin paper or glossy paper, and the said substrate of laying eggs is enclosed in the bottle mouth position of said interior bottle through elastic cord.
Preferably, the fine sand of said interior bottle bottom placement sterilization.
Preferably, TF is set also on the said fine-meshed screen, said TF is the U type, and the middle part of said TF is porose, is inserted with cotton-wool in the said hole.
Said outer cover is flat big open end bottle, tube, bucket, box or case, comprises the tube that flat chassis and flexible plastic sheet surround, and an end of the tube that said flexible plastic sheet surrounds stands in the said flat chassis.
Compared with prior art, the method for corn borer breed in grades of the present invention divides two-stage to raise larva, can reject sick and weak individuality, keeps polypide healthy and strong, neat; Wherein, the papery device of pupating has improved the efficient of pupating, and the stripping pupa is also very convenient; Eliminate choosing twice through larva and pupa, can improve the regularity of whole raising population, keep individual robustness, not only can improve contemporary feeding efficiency, also can guarantee continuous subculture feeding effect.The raising device of a kind of corn borer adult that the present invention relates to is simple in structure, with low cost, operates simple and easy, convenient and practical.The sterilization fine sand layer of interior bottle bottom links to each other with the sterile water of jacket bottom through a bottle ins and outs hole, can keep fine sand moistening, and the evaporation of the Shamian Island and the jacket bottom water surface can guarantee pupa emergence, adult mating and lay eggs to the requirement of humidity.Vacancy section accounts for the major part of whole bottle body, and gauze screen is set, and like this, both can guarantee that bottle interior air permeability and good also can avoid adult that ovum is originated on the gauze in a large number.Bottle middle part in the TF of U-shaped is arranged at through pore; Need not device for opening and can inject hydromel through the mouth of pipe; The suitable injection rate of control every day not only can guarantee hydromel fresh (not residual in a large number) but also can guarantee that adult sucks (cotton-wool is not dry) at any time, compares with the conventional method of changing cotton balls every day; Not only easy to operate, and feeding efficient is improved.At the bottle mouth position setting substrate of laying eggs, and be placed in the bottle mouth position of said plastic bottle, such as rubber band through elastic cord; Be convenient to adult and ovum originated on the inner surface of the substrate of laying eggs, change every day when laying eggs substrate, need interior bottle is taken out from overcoat; Untie elastic cord; New substrate is placed on the old substrate, push down gently and old substrate is slowly extracted out, tighten again and put into overcoat and get final product.So operation is not only easy, but also can avoid adult to escape.Overcoat of the present invention need add an amount of sterile water; To keep horizontal plane to be advisable, through the osmosis of interior bottle bottom margin aperture, can guarantee the moistening of fine sand on the one hand a little less than the sapin face of interior bottle bottom; The scale that comes off of adult is through the iris action of outer cover top wet gauze on the other hand; Also can sink in the water or in the wet sand, thereby avoid scale to fly upward everywhere, not only can keep raising the cleaning of subenvironment but also can avoid keeper's mistake to inhale that scale gets into respiratory tract and unhealthful.In bottle diameter and overcoat diameter with 1: 1.5-2.0 is advisable, and so not only can keep preferably gas permeability but also can keep air humidity preferably.Corn borer imago breeding device of the present invention also applicable to the mating of the emergence of other lepidopterous insects pupas such as cotton bollworm, beet armyworm, prodenia litura, adult with the raising activity such as lay eggs.
Description of drawings
Fig. 1 is the side schematic view of a preferred embodiment of the interior bottle in the raising device of corn borer adult of the present invention;
Fig. 2 is the sketch map of a preferred embodiment of the TF of the interior bottle in the raising device of corn borer adult of the present invention;
Fig. 3 is the side schematic view of a preferred embodiment of the outer cover in the raising device of corn borer adult of the present invention;
Fig. 4 is the side schematic view of another preferred embodiment of the outer cover in the raising device of corn borer adult of the present invention;
Fig. 5 is the generalized section of a preferred embodiment of the raising device of corn borer adult of the present invention;
Fig. 6 is the generalized section of another preferred embodiment of the raising device of corn borer adult of the present invention.
Embodiment
For making the present invention be more prone to understand,, further set forth the present invention below in conjunction with specific embodiment.Should be understood that these embodiment only to be used to the present invention is described and be not used in the restriction scope of the present invention.
A preferred embodiment as the breed in grades method of corn borer of the present invention; Concrete operations are following: at first, every hole adds an amount of corn borer artificial feed piece in the 12 porocyte culture plates, and the head into newly hatched larvae 5-10 is chosen in every hole; The culture plate lid of pad blowing paper is raised down in 25 ± 1 ℃ in adding a cover.After 6-7 days, select neat, the healthy and strong larva of growth and change in the tissue culture flasks, every bottle of 20-30 head is raised down in 25 ± 1 ℃;
when treating that larva grew to for 5 ages, adds the papery device of pupating in the tissue culture flasks in said step (2); Mature larva can pierce automatically pupates; After waiting to pupate, strip and select healthy and strong pupa and go into imago cage.Wherein, the papery device of pupating is a paper cylinder, and cylinder has corrugated wrinkle.
Fig. 1 is the side schematic view of a preferred embodiment of the interior bottle in the raising device of corn borer adult of the present invention.As shown in Figure 1, the interior bottle in the raising device of this corn borer adult is uncovered plastic bottle, and body has vacancy section 3, and bottleneck has the substrate of laying eggs 1, and the substrate 1 of laying eggs is enclosed in the bottle mouth position of plastic bottle through rubber band.Vacancy section 3 accounts for the major part of whole bottle body, and built-in gauze screen 2 so not only can increase the gas permeability of cage body but also can avoid adult that ovum is originated on bottle inwall in a large number.Bottle adds the heat-killed fine sand 4 of proper amount of dry in the end, plays dual parts to preserve moisture and fixing fine-meshed screen.Aperture 6 is arranged at the plastic bottle bottom.For the ease of feeding, on gauze screen 2, also inserted TF 5, the two ends of TF 5 protrude in outside the interior bottle.Fig. 2 is the sketch map of a preferred embodiment of TF, and by the structure of the visible TF 5 of Fig. 2, TF 5 is the U type in interior bottle, and cotton 52 is filled in center perforate 51 in the hole 51.During feeding, need not device for opening, promptly available syringe or dropper inject TF 5 with hydromel, and hydromel soaks cotton, sucks at any time for adult.For guaranteeing the fresh of hydromel, the injection rate of hydromel can have been sucked in one day with adult in the cage and has been advisable in the TF, and the fresh hydromel of injection every day.In the present embodiment, for raising corn borer, its gauze screen 2 is a 10-20 order gauze screen.The bottle mouth position of plastic bottle has the substrate of laying eggs 1, and this substrate 1 of laying eggs is preferably paraffin paper.
Fig. 3 is the side schematic view of a preferred embodiment of the outer cover in the raising device of corn borer adult; Outer cover shown in Figure 3 is a flat uncovered plastic bottle body 7, and the outer cover opening end covers a moistening white gauze 8.Add an amount of sterile water in the bottle, keep horizontal plane to be advisable a little less than interior bottle sapin face, like this through the osmosis of aperture 6, the suitable humidity of bottle fine sand in can guaranteeing.Like this, pupa to be sprouted wings places the smooth emergence that helps pupa on the moistening Shamian Island; Simultaneously, wet husky evaporation with the outer cover bottom water can guarantee adult mating, the suitable humidity of laying eggs; Through the iris action of the white gauze of outer cover opening end, the scale that adult comes off can fall into wet sand 4 or water 10, can prevent that scale from flying upward.
Fig. 4 is the side schematic view of another preferred embodiment of the outer cover in the raising device of corn borer adult of the present invention; Outer outlet body 7 is to be rolled into cylindrical shape or square forming by a flexible plastic sheet.Outer outlet body 7 port are provided with white gauze 8, and bottom nozzle places on the flat chassis 9, and flat chassis 9 can replace with culture dish or enamel tray, adds an amount of sterile water in the dish.
Fig. 5 is the generalized section of the preferred embodiment of the raising device of corn borer adult of the present invention when using, and this device is the device that bottle is joined an outer cover in.The diameter of outer cover is 1.7 times of interior bottle, and interior bottle is positioned in the outer cover, and interior bottle is uncovered plastic bottle, and body has vacancy section 3, and bottleneck has the substrate of laying eggs 1, and the substrate 1 of laying eggs is enclosed in the bottle mouth position of said plastic bottle through rubber band.Vacancy section 3 accounts for the major part of whole bottle body, in 10 order gauze screens 2 are set, so not only can increase the gas permeability of cage body but also can avoid adult that ovum is originated on bottle inwall in a large number.For the ease of feeding, on fine-meshed screen 2, also inserted TF 5, the two ends of TF 5 protrude in outside the interior bottle.TF 5 is the U type in interior bottle, cotton is filled in the center perforate in the hole.During feeding, need not device for opening, promptly available syringe or dropper inject TF 5 with hydromel, and hydromel soaks cotton, sucks at any time for adult.For guaranteeing the fresh of hydromel, the injection rate of hydromel can have been sucked in one day with adult in the cage and has been advisable in the TF, and the fresh hydromel of injection every day.This substrate 1 of laying eggs is paraffin paper; Hot air sterilization fine sand 4 is placed in the plastic bottle bottom, in the plastic bottle bottom aperture 6 is arranged simultaneously.Outer outlet body 7 is flat uncovered plastic bottles, adds an amount of sterile water in the bottle, keeps horizontal plane to be advisable a little less than interior bottle sapin face, like this through the osmosis of aperture 6, and the suitable humidity of bottle fine sand in can guaranteeing.The outer cover opening end covers a moistening white gauze 8.Like this, pupa to be sprouted wings places the smooth emergence that helps pupa on the moistening Shamian Island; Simultaneously, wet husky evaporation with the outer cover bottom water can guarantee adult mating, the suitable humidity of laying eggs; Through the iris action of the white gauze of outer cover opening end, the scale that adult comes off can fall into wet sand 4 or water 10, can prevent that scale from flying upward, and not only can keep the feeding environment cleaning but also can avoid scale to be strayed into keeper's respiratory tract.
Fig. 6 is the generalized section of another preferred embodiment of the raising device of corn borer adult of the present invention when using, and this device is the device of the shared outer covers of bottle in a plurality of.The shape and structure of interior bottle is with the same shown in Fig. 5.Interior bottle also can use the flat mineral water bottle of 1.5L to make, the about 9cm of diameter, high about 19cm.Flat chassis 9 can use the square enamel tray of 42 * 32cm to replace, and outer outlet body 7 is the square tubes that are converted into by a flexible plastic sheet, and length and width is more smaller than flat chassis, high about 22cm.Outer outlet body 7 places on the flat chassis 9, and it is an amount of to add water, divides 2 rows, 3 row to be positioned in the outer outlet body one on the white gauze of outer outlet body port humidification in 6 above-mentioned interior bottles simultaneously.The outer cover also cuboid transparent plastic storage tank of available suitable size replaces, and throws off case lid, puts into bottle, adds water, covers white gauze and gets final product.
The raising device of corn borer adult of the present invention also applicable to the mating of the emergence of other lepidopterous insects pupas such as cotton bollworm, beet armyworm, prodenia litura, adult with lay eggs.
Last institute should be noted that; Above embodiment is only in order to technical scheme of the present invention to be described but not to the restriction of protection domain of the present invention; Although the present invention has been done detailed description with reference to preferred embodiment; Those of ordinary skill in the art should be appreciated that and can make amendment or be equal to replacement technical scheme of the present invention, and do not break away from the essence and the scope of technical scheme of the present invention.
Claims (4)
1. the breed in grades method of a corn borer is characterized in that, said method specifically comprises the steps:
(1) the corn borer newly hatched larvae is positioned in the 12 porocyte culture plates raises;
(2) after 6-7 days, select neat, the healthy and strong corn borer larvae of growth and change in the tissue culture flasks and raise;
When (3) treating that larva grows to mature larva, add the papery device of pupating in the tissue culture flasks in said step (2);
(4) wait to pupate after, strip and select healthy and strong pupa and go into imago cage.
2. the breed in grades method of a kind of corn borer according to claim 1 is characterized in that, puts into 5-10 newly hatched larvae in every hole of the Tissue Culture Plate in the said step (1).
3. the breed in grades method of a kind of corn borer according to claim 1 is characterized in that, the tissue culture flasks in the said step (2) is put into the 20-30 head through eliminating the larva of choosing for every bottle.
4. the breed in grades method of a kind of corn borer according to claim 1 is characterized in that, the device of pupating of the papery in the said step (3) is a paper cylinder, and said cylinder has the groove wrinkle.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009100421921A CN101642079B (en) | 2009-08-27 | 2009-08-27 | Classified raising method and imago raising device of corn borers |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009100421921A CN101642079B (en) | 2009-08-27 | 2009-08-27 | Classified raising method and imago raising device of corn borers |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101642079A CN101642079A (en) | 2010-02-10 |
| CN101642079B true CN101642079B (en) | 2012-05-30 |
Family
ID=41654261
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2009100421921A Expired - Fee Related CN101642079B (en) | 2009-08-27 | 2009-08-27 | Classified raising method and imago raising device of corn borers |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101642079B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107006431A (en) * | 2017-03-29 | 2017-08-04 | 江西农业大学 | A kind of new Ostrinia furnacalis breeding apparatus and its operating method |
Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103609531B (en) * | 2013-11-21 | 2015-03-04 | 北京林业大学 | Artificial feeding method of fragrant pear euzophera pyriella |
| CN104094897B (en) * | 2014-02-17 | 2016-01-20 | 中国农业科学院植物保护研究所 | Beet webworm imago breeding utensil |
| CN103960204B (en) * | 2014-05-30 | 2015-08-19 | 青岛农业大学 | A kind of method of raising lepidopterous insects |
| CN104322461B (en) * | 2014-11-15 | 2016-01-27 | 云南省农业科学院甘蔗研究所 | A kind of method that pink rice borer indoors artificial is raised |
| CN104604814B (en) * | 2015-02-17 | 2017-01-25 | 浙江省农业科学院 | Method for efficient chilo suppressalis breeding |
| FR3034623B1 (en) * | 2015-04-13 | 2017-04-14 | Ynsect | INSECT BREEDING METHOD |
| CN105028333B (en) * | 2015-06-25 | 2017-07-21 | 吉林省林业科学研究院 | A kind of method of artificial room's mass rearing Berry size |
| CN105494269B (en) * | 2015-12-29 | 2021-04-27 | 广西壮族自治区农业科学院甘蔗研究所 | Method for raising sugarcane dibbler |
| CN105766809B (en) * | 2016-04-05 | 2018-10-19 | 南昌大学 | A kind of small arthropod life table experimental method |
| CN105994170A (en) * | 2016-05-31 | 2016-10-12 | 中国农业科学院植物保护研究所 | Device and method for improving artificial feeding efficiency of cotton bollworms |
| CN106719459B (en) * | 2017-01-11 | 2023-05-30 | 贵州大学 | Piercing-sucking mouth gag insect fluid feed feeding frame and feeding method |
| CN111449028B (en) * | 2020-06-09 | 2022-01-11 | 贵阳学院 | Winter laboratory breeding method for Spodoptera frugiperda |
| CN112369375B (en) * | 2020-11-26 | 2022-07-15 | 云南林业职业技术学院 | Method for breeding mantis yunnanensis |
-
2009
- 2009-08-27 CN CN2009100421921A patent/CN101642079B/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107006431A (en) * | 2017-03-29 | 2017-08-04 | 江西农业大学 | A kind of new Ostrinia furnacalis breeding apparatus and its operating method |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101642079A (en) | 2010-02-10 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101642079B (en) | Classified raising method and imago raising device of corn borers | |
| CN201509510U (en) | Adult lepidopteron feeding device | |
| CN201185613Y (en) | Device for breeding and collecting parasitic insect | |
| CN107568170B (en) | Large-scale artificial feeding method for lugworm bugs | |
| CN103960204A (en) | Method for feeding lepidopteron | |
| CN204994448U (en) | Box is raised to bollworm | |
| CN101622971A (en) | Technology for artificially raising and breeding Dastarcus helophoroides of Anoplophora malasiaca and preventing and controlling Anoplophora glabripennis | |
| CN102125002A (en) | Method for artificially raising plant bug parasitic wasps | |
| CN112042598B (en) | Whole set method for breeding harmonia axyridis | |
| CN108012995B (en) | Indoor breeding method and device for Diaphania ciliata | |
| CN103918613A (en) | Pupal-stage parasitic wasp artificial-propagation method, wasp-keeping box and manufacturing method of wasp-keeping box | |
| CN110679550A (en) | Large-scale artificial breeding method for ceratophaga xylostella | |
| CN205390042U (en) | Container is raised to tiny flower stinkbug in east asia | |
| CN105211006B (en) | Parasitic wasp class natural enemy release cup and parasitic wasp class natural enemy sustained release method | |
| CN105165800B (en) | Parasitic wasp class natural enemy release device and parasitic wasp class natural enemy sustained release method | |
| CN204742254U (en) | A support worm cage that is used for scale of predatism natural enemy arma custos to raise | |
| CN113519468A (en) | Method for feeding phyllotreta striolata in indoor generation | |
| CN110876360B (en) | Armyworm feeding device and method | |
| CN209300025U (en) | A kind of sericulture device | |
| CN105393986B (en) | A method of the parasitic wasp of release Chilo spp larvae | |
| CN106719466B (en) | The pupa of bat moth-moth integrated nursing care device and care method | |
| CN112400811A (en) | Method for breeding dastarcus helophoroides on large scale by utilizing thyestillageblerifolius | |
| CN108094331A (en) | A kind of narrow edge applies the indoor feeding method of noctuid | |
| CN107494459B (en) | Breeding method of wild silkworm larvae | |
| CN213603897U (en) | A raise device for harmonia axyridis |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20120530 Termination date: 20130827 |