CN105994170A - Device and method for improving artificial feeding efficiency of cotton bollworms - Google Patents
Device and method for improving artificial feeding efficiency of cotton bollworms Download PDFInfo
- Publication number
- CN105994170A CN105994170A CN201610375941.2A CN201610375941A CN105994170A CN 105994170 A CN105994170 A CN 105994170A CN 201610375941 A CN201610375941 A CN 201610375941A CN 105994170 A CN105994170 A CN 105994170A
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- Prior art keywords
- bollworm
- blow molding
- improving
- bred
- larvae
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- 241000255967 Helicoverpa zea Species 0.000 title claims abstract description 40
- 238000000034 method Methods 0.000 title claims abstract description 18
- 238000009395 breeding Methods 0.000 claims abstract description 19
- 230000001488 breeding effect Effects 0.000 claims abstract description 19
- 238000007664 blowing Methods 0.000 claims description 16
- 230000012010 growth Effects 0.000 claims description 7
- 239000011521 glass Substances 0.000 abstract description 4
- 206010011409 Cross infection Diseases 0.000 abstract description 3
- 206010029803 Nosocomial infection Diseases 0.000 abstract description 3
- 238000011161 development Methods 0.000 abstract description 3
- 230000000694 effects Effects 0.000 abstract description 3
- 241000238631 Hexapoda Species 0.000 abstract description 2
- 230000009286 beneficial effect Effects 0.000 abstract description 2
- 238000000071 blow moulding Methods 0.000 abstract 5
- 239000011148 porous material Substances 0.000 abstract 3
- 238000005516 engineering process Methods 0.000 description 4
- 230000006872 improvement Effects 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 235000011158 Prunus mume Nutrition 0.000 description 2
- 244000018795 Prunus mume Species 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 239000010985 leather Substances 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 230000004083 survival effect Effects 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- 229920000742 Cotton Polymers 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 230000012447 hatching Effects 0.000 description 1
- 239000002917 insecticide Substances 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 230000009571 larval growth Effects 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 230000009456 molecular mechanism Effects 0.000 description 1
- 230000017448 oviposition Effects 0.000 description 1
- 239000002574 poison Substances 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 238000012353 t test Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention relates to insect breeding, and particularly discloses a device and method for improving artificial feeding efficiency of cotton bollworms. The device mainly comprises a pore plate. Blow molding paper with the same size as an upper cover is added into the upper cover of the pore plate. The method comprises the steps that cotton bollworms are bred through the device to third instar larvae, and third instar larvae growing uniformly are selected and transferred into a bollworm breeding pipe to continue to be bred to pupate. In order to solve the problems that young cotton bollworm larvae are high in escape rate, uneven in development progress, prone to cross infection and the like, newly hatched larvae are bred in the 24-pore plate, the blow molding paper is added for prevent escape, and third instar larvae are transferred into the glass bollworm breeding pipe to be bred. By means of difference between the effect obtained by adding the blow molding paper and the effect obtained by adding no blow molding paper, it is proved that the blow molding paper has the beneficial effect on lowering the larva escape rate and improving development uniformity.
Description
Technical field
The present invention relates to insecticide raise, specifically, relate to the artificial breeding of bollworm.
Background technology
Bollworm is the important pests of various crop of causing harm, and people are around the ecology of bollworm, life
Change mechanism, molecular mechanism, Prevention Technique etc. and carried out a lot of research, therefore, advise greatly in indoor
Mould artificial breeding bollworm, timely offer for test try the important leverage that worm is every research.
Substantially increase after within 1999, the feed formula of bollworm is improved by our laboratory
The survival rate of bollworm, egg laying amount, egg hatching rate etc., the apparatus selecting breeding grub at that time is
The flat bottom glass pipe of long 7.5cm diameter 2.5cm, directly receives raising in glass tubing by newly hatched larvae
On material, often pipe 1-2 head, more uniform for growth 3 instar larvaes are chosen from pipe after 7 days, transfer
Continue to raise in the pipe install new feedstuff；This method needs the most more, relatively costly (beam
Leather prunus mume (sieb.) sieb.et zucc. etc., 1999).Bollworm method for breeding is improved by the virtuous woodss of model in 2003 etc., proposes
Newly hatched larvae is chosen 10 holes and raises in boxes, 1, every hole, disposably put into man-made feeds,
Until pupating；But the shortcoming of this method is that the mortality rate of newly hatched larvae is higher, and larva is raw
Long concordance of growing is the highest, causes pupate irregular (the virtuous woods of model etc., 2003).2015
Bollworm breeding technology is improved (Qin Qilian, 2015) by Qin Qilian again, proposes with supporting
Low instar larvae raised by worm tank, raises, but once support in worm tank after 3 ages in proceeding to 24 hole insect box
Thoughts catch an illness poison etc. larva, may whole tank larva all can be affected.
Summary of the invention
In order to solve problems of the prior art, it is an object of the invention to provide a kind of raising
The apparatus and method of bollworm artificial breeding's efficiency.
In order to realize the object of the invention, technical scheme is as follows:
First aspect, the present invention provides a kind of device improving bollworm artificial breeding's efficiency, institute
The main body stating device is orifice plate, adds the blowing paper equivalently-sized with upper cover in the upper cover of orifice plate.
As preferably, described orifice plate is 24 orifice plates.
Further, described blowing paper is 1-2 layer, and thickness reaches 1.5-2mm.
Second aspect, the present invention provides a kind of method improving bollworm artificial breeding's efficiency, i.e.
Utilize aforementioned means feeding cotton bollworms to 3 instar larvaes, then select 3 consistent instar larvaes of growth and turn
Move on to support in scolite and continue to raise to pupating.
The present invention is found by experiment that, raises cotton boll when utilizing the upper cover 24 orifice plates added with blowing paper
During worm larva, can effectively reduce the escapement ratio of cotton bollworm larvae, and improve growth uniformity.
Further, in order to prevent the problem of the easy cross infection of cotton bollworm larvae in feeding process,
Feeding cotton bollworms, to the process of 3 instar larvaes particularly as follows: put into man-made feeds in 24 orifice plates, connects
The larva just incubated, in 24 orifice plates, covers the lid added with blowing paper, after device is bundled,
Temperature 27 ± 1 DEG C, relative humidity 70 ± 10%, raise under conditions of photoperiod 14L:10D.
As preferably, in order to ensure the growth of cotton bollworm larvae, every hole is put into 1～2g and manually raises
Material, connects the larva incubated at the beginning of 3～4.
Man-made feeds can be selected for the feedstuff of bollworm commonly used in the art, is preferably " artificial breeding
The improvement of bollworm technology " disclosed in (beam leather prunus mume (sieb.) sieb.et zucc. etc., plant protection, the 2nd phase in 1999)
Man-made feeds formula 4.
The third aspect, the invention provides described device and is improving bollworm artificial breeding efficiency side
The application in face.
The beneficial effects of the present invention is:
The present invention is directed to low instar bollworm grub escapement ratio height, development progress uneven, the easy intersection is felt
The problems such as dye, propose newly hatched larvae and raise in 24 orifice plates, increase blowing paper and prevent from escaping, and 3
Transfer to glass after age and support the technology raised in scolite.So, integrated cost is relatively low, and efficiency is relatively
Height, and can more effectively prevent cross infection between larva.
Accompanying drawing explanation
Fig. 1 is interior 24 orifice plates added with blowing paper of upper cover of the present invention.
Whether Fig. 2 is added with being blow molded paper to bollworm escape (left) and the shadow of regularity (right)
Ring.
Detailed description of the invention
Below in conjunction with embodiment, the preferred embodiment of the present invention is described in detail.Need
Being understood by providing merely to play descriptive purpose of following example, it is right to be not used to
The scope of the present invention limits.Those skilled in the art without departing substantially from spirit of the invention and
In the case of spirit, the present invention can be carried out various amendment and replacement.
Experimental technique used in following embodiment if no special instructions, is routine side
Method.
Material used in following embodiment, reagent etc., if no special instructions, all can be from business
Industry approach obtains.
Embodiment 1
The present embodiment is for illustrating a kind of device improving bollworm artificial breeding's efficiency.
The main body of described device is 24 orifice plates, according on the size of 24 orifice plates, cutting and 24 orifice plates
One, the blowing paper (purchase of common stationer's) that lid size is identical, puts into upper cover.
Embodiment 2
The present embodiment is for illustrating a kind of method improving bollworm artificial breeding's efficiency.
The man-made feeds taking 1g-2g are cultivated in box in 24 holes of device described in embodiment 1, connect and just incubate
Cotton bollworm larvae in 24 orifice plates, every hole connects 3, closes the lid, with rubber band bundle after
It is put in temperature 27 ± 1 DEG C, relative humidity 70 ± 10%, raises under conditions of photoperiod 14L:10D；
Often process (Fig. 1) in triplicate.Directly cover 24 orifice plates of upper cover and be processed as being not added with being blow molded paper
Comparison.
Observe the cotton bollworm larvae number in 24 orifice plates every day period, grow age, SPSS 18.0
Software carries out significance of difference analysis, and the difference adding blowing paper with being not added with being blow molded paper is compared in t test,
Evaluate and utilize blowing paper to reducing larva escapement ratio, the effect of raising growth uniformity.
According to measurement result (Fig. 2), the escape of bollworm after adding blowing paper, can be significantly reduced
Rate, especially after larval growth to 2 ages, i.e. connect the 5th after worm, 6,7 days, add blowing paper at
In 24 orifice plates of reason, the bollworm of survival is than comparison the most 13.39% (P=0.021) of component,
27.97% (P=0.016) and 53.88% (P < 0.001).Meanwhile, cover add blowing paper permissible
Significantly improve the growth uniformity of cotton bollworm larvae, after connecing worm the 7th day, add the place of blowing paper
Reason group 3 bollworm in age accounts for always deposits the 62.13% of the 88.18% of borer population, significantly higher than matched group
(P<0.001)。
Although, the most with a general description of the specific embodiments the present invention has been made in detail
Most description, but on the basis of the present invention, it can be made some modifications or improvements, this is to this
It is apparent from for skilled person.Therefore, on the basis without departing from spirit of the present invention
Upper these modifications or improvements, belong to the scope of protection of present invention.
Claims (7)
1. the device improving bollworm artificial breeding's efficiency, it is characterised in that described dress
The main body put is orifice plate, adds the blowing paper equivalently-sized with upper cover in the upper cover of orifice plate.
Device the most according to claim 1, it is characterised in that described orifice plate is 24 holes
Plate.
Device the most according to claim 2, it is characterised in that described blowing paper is 1-2
Layer.
4. the method improving bollworm artificial breeding's efficiency, it is characterised in that exploitation right
Profit requires that the device feeding cotton bollworms described in 1～3 any one, to 3 instar larvaes, selects growth consistent
3 instar larvaes are transferred to support in scolite and are continued to raise to pupating.
Method the most according to claim 4, it is characterised in that put into people in 24 orifice plates
Work feedstuff, the larva incubated at the beginning of connecing, in 24 orifice plates, covers the lid added with blowing paper, by device
After binding, in temperature 27 ± 1 DEG C, relative humidity 70 ± 10%, the condition of photoperiod 14L:10D
Lower raising.
Method the most according to claim 5, it is characterised in that put into 1～2g in every hole
Man-made feeds, connect the larva incubated at the beginning of 3～4.
7. the device described in any one of claims 1 to 3 is improving bollworm artificial breeding's efficiency
The application of aspect.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610375941.2A CN105994170A (en) | 2016-05-31 | 2016-05-31 | Device and method for improving artificial feeding efficiency of cotton bollworms |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610375941.2A CN105994170A (en) | 2016-05-31 | 2016-05-31 | Device and method for improving artificial feeding efficiency of cotton bollworms |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105994170A true CN105994170A (en) | 2016-10-12 |
Family
ID=57091865
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610375941.2A Pending CN105994170A (en) | 2016-05-31 | 2016-05-31 | Device and method for improving artificial feeding efficiency of cotton bollworms |
Country Status (1)
| Country | Link |
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| CN (1) | CN105994170A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110558294A (en) * | 2019-09-29 | 2019-12-13 | 重庆市农业科学院 | Simple and efficient two-stage harmonia axyridis breeding method |
| CN115530127A (en) * | 2022-06-08 | 2022-12-30 | 四川省农业科学院植物保护研究所 | Group breeding method for cotton bollworms |
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|---|---|---|---|---|
| CN1160494A (en) * | 1997-01-27 | 1997-10-01 | 中国农业科学院植物保护研究所 | Feed for large-scale raising of corn borer |
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| CN102643773A (en) * | 2011-02-18 | 2012-08-22 | 华中农业大学 | Screening and identification of genetically engineered Bacillus thuringiensis YBT-881-L1 of overexpressed CodY protein |
| CN103267865A (en) * | 2013-05-22 | 2013-08-28 | 中国农业科学院棉花研究所 | Method for detecting Bt protein resistance of cotton bollworm |
| CN103734096A (en) * | 2014-01-20 | 2014-04-23 | 中国热带农业科学院环境与植物保护研究所 | Feeding and propagation-expanding method for chelisodochidae |
| CN104845984A (en) * | 2015-06-05 | 2015-08-19 | 东北农业大学 | vip3A-1 gene of bacillus thuringiensis expression protein with high toxicity for asparagus caterpillar |
| CN104845983A (en) * | 2015-06-05 | 2015-08-19 | 东北农业大学 | Bacillus thuringiensis vip3A-6 genes, expression protein and application thereof |
| CN104962577A (en) * | 2015-06-17 | 2015-10-07 | 湖北省生物农药工程研究中心 | Bacillus one-pot molecular breeding method and application thereof |
| CN105348374A (en) * | 2015-12-01 | 2016-02-24 | 中国农业科学院植物保护研究所 | Method for acquiring high-activity Cry1Ai protein mutants and mutants |
| CN105394457A (en) * | 2015-11-24 | 2016-03-16 | 中国农业科学院棉花研究所 | Feed for artificial breeding of cotton bollworm, preparation method for feed, and breeding method |
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2016
- 2016-05-31 CN CN201610375941.2A patent/CN105994170A/en active Pending
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1160494A (en) * | 1997-01-27 | 1997-10-01 | 中国农业科学院植物保护研究所 | Feed for large-scale raising of corn borer |
| CN101642079A (en) * | 2009-08-27 | 2010-02-10 | 广东省农业科学院作物研究所 | Classified raising method and imago raising device of corn borers |
| CN102643773A (en) * | 2011-02-18 | 2012-08-22 | 华中农业大学 | Screening and identification of genetically engineered Bacillus thuringiensis YBT-881-L1 of overexpressed CodY protein |
| CN103267865A (en) * | 2013-05-22 | 2013-08-28 | 中国农业科学院棉花研究所 | Method for detecting Bt protein resistance of cotton bollworm |
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| CN104845984A (en) * | 2015-06-05 | 2015-08-19 | 东北农业大学 | vip3A-1 gene of bacillus thuringiensis expression protein with high toxicity for asparagus caterpillar |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110558294A (en) * | 2019-09-29 | 2019-12-13 | 重庆市农业科学院 | Simple and efficient two-stage harmonia axyridis breeding method |
| CN115530127A (en) * | 2022-06-08 | 2022-12-30 | 四川省农业科学院植物保护研究所 | Group breeding method for cotton bollworms |
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Application publication date: 20161012 |