KR102113850B1 - Method for artificial spawn of Zophobas atratus Fabricius - Google Patents

Abstract

The present invention relates to a method for artificial spawning of the American king and giraffe.
Artificial spawning method of the American king meal of the present invention includes (a) photoperiod 13L / 11D to 15L / 9D; (b) It is characterized by inducing artificial spawning of the American kingfish under the conditions of temperature 27 ° C to 30 ° C;
According to the present invention, it is possible to improve the spawning characteristics of the American king-fly and improve the spawning rate and shorten the spawning period. It is easy to use and can be expected to increase labor efficiency.

Description

Method for artificial spawn of Zophobas atratus Fabricius}

The present invention relates to a method for artificially spawning an American kingfish, and more specifically, to increase the spawning rate in a shorter time by inducing artificial spawning of an American kingfish through specific temperature, photoperiod adjustment, and introduction of a specific spawning place by age. It is about the method of artificial spawning of the American Great Pheasant.

Insects are one of the important roles in the natural ecosystem, which are used as animal feed, because many insect species are nutritionally rich. In Korea, it has been used as a feed for pets for more than 10 years, and has started breeding in insect lovers and zoos. Recently, as a feed insect, a number of farms are growing for industrialization due to various advantages.

In particular, the insect used for animal feed, which is widely used in the past, is the insect of the family Pegasus ( Tenebrionidae ). Several studies have been conducted on the growth characteristics, artificial breeding methods, and artificial feed of these insects.

Related prior arts include Korean Registered Patent No. 10-1660379 (How to shorten the development of brown larvae larvae), Korean Patent Publication No. 10-2017-0052128 (Feed composition for breeding larvae larvae), Korean Registered Patent No. 10-1663202 (Insect mass breeding equipment and facilities) and the like are known.

Although several studies have been conducted on the beetle and insects, most of them mention only the developmental characteristics of the larvae or adults of the brownish beetle. The study has not been conducted.

An object of the present invention is to provide an artificial spawning method of an American kingfish that can be mass-produced while easily using it at a farm by identifying optimal spawning conditions that can increase the spawning rate in a shorter time by inducing artificial spawning of the American kingfish. Has to do.

In order to achieve the above object, artificial spawning methods of the American king-fly of the present invention include (a) photoperiod 13L / 11D to 15L / 9D; (b) It is characterized by inducing artificial spawning of the American kingfish under the conditions of temperature 27 ° C to 30 ° C;

The American king meal is characterized by inducing artificial spawning by applying 70 to 80% of the general spawning ground with bran from more than 35 days of spawning.

The American king meal is characterized by inducing spawning at a temperature of 29 to 30 ° C from 60 to 90 days.

The American king meal is characterized in that it induces scattering in a photoperiod of 14L / 10D to 15L / 9D, and induces scattering under conditions of 70 to 80% humidity and 1,330 to 1800 Lux.

When the spawning induction condition is applied, the adult adult mealworm is characterized by an average number of spawning per day from 20 days to 90 days after spawning, and lays 500 to 650 eggs during 90 to 100 days. .

The technical problems to be achieved by the present invention are not limited to the technical problems mentioned above.

According to the present invention, it is possible to improve the spawning characteristics of the American king-fly and improve the spawning rate and shorten the spawning period. In particular, it is possible to lower the mortality rate of adult insects, and thus it is possible to farm the farm by identifying the optimum spawning conditions that show a higher amount of spawning rate. It is easy to use and can be expected to increase labor efficiency.

1 is a graph showing the cumulative number of eggs laid by one female per spawning day according to the Gwangju period.
2 is a graph showing the cumulative mortality rate of each adult spawning adult according to the Gwangju period.
Figure 3 is a graph showing the average number of eggs per female per day of spawning of the American king-fly according to temperature.
4 is a graph showing the cumulative number of eggs per female per spawning day of the American king-fly according to temperature.
5 is a graph showing the lifespan of a female American and adult female according to temperature.
FIG. 6 is a graph showing the lifespan of male and female adults by spawning day according to temperature.
FIG. 7 is a graph showing the spawning ratio according to the spawning location of the American kings by spawning day according to temperature.

Hereinafter, the present invention will be described in detail, and detailed descriptions of well-known functions and configurations that may unnecessarily obscure the subject matter of the present invention will be omitted.

In order to achieve the above technical problem, the present invention is a result of several studies to find the optimal scattering conditions of the American king and nerd, (a) Gwangjugi 13L / 11D to 15L / 9D; (b) It is found that artificial spawning of the American king-eater under the conditions of temperature of 27 ° C to 30 ° C; shortens the spawning period of the American king-eater while lowering the mortality rate of the adult and increasing the spawning rate.

Hereinafter, a detailed description of the artificial spawning method of the American king meal of the present invention is as follows.

All insects are significantly affected by the spawning status and spawning rate depending on the environmental conditions applied during artificial spawning, and the effect on each spawning period is different.

Accordingly, in the present invention, in order to increase the spawning rate and lower the mortality rate of the adult mealworm in the case of artificial spawning by inducing spawning of the adult mealworm, (a) Gwangju period 13L / 11D to 15L / 9D; (b) Characterized by inducing spawning in an environment at a temperature of 27 ° C to 30 ° C. When these conditions are exceeded, the mortality rate of adult adults is high and the spawning rate itself falls. It is an essential condition to be, more preferably (a) photoperiod 14L / 10D to 15L / 9D; (b) temperature from 29 to 30 ° C from 60 to 90 days of scattering; (c) Humidity of 70 ~ 80% (d) It is best to induce scattering under conditions of illuminance 1,330 ~ 1800 Lux.

Particularly, when the photoperiod is less than 13L / 11D, it is difficult to obtain a constant spawning rate because it contains a photoperiod having a low spawning rate. In particular, males show a relatively high mortality rate compared to the entire male under the 9L / 15D photoperiod. It is impossible to breed adults without distinction between male and female, and when it exceeds 15L / 9D, it is difficult to obtain an increased scattering rate compared to the condition of 15L / 9D, which is economically inefficient, and when the temperature exceeds 30 ° C 1 The average number of eggs per day is less than 20, and the spawning rate is rather low. When the temperature is less than 27 ° C, the mortality rate of female adults is high, so that the induction of spawning falls rapidly, making it difficult to achieve the desired effect of the present invention.

In addition, in the present invention, the spawning ground also has a great influence on the spawning rate, and the spawning rate is confirmed for each spawning day according to the spawning site. From one time later, it was confirmed that the spawning rate was higher in the spawning site containing bran.

Thus, in order to obtain higher spawning rates, as well as specific photoperiod, temperature, humidity, and illuminance conditions identified as the spawning induction conditions, 70-80% of the general spawning ground is applied with bran from more than 35 days of spawning. By inducing artificial scattering, the highest scattering rate can be obtained in a short period of time.

Under these spawning conditions, it was confirmed that the American king meal lays 20 to 30 eggs per day from the 25th day to the 90th day of spawning, and lays 500 to 650 eggs from 90 to 100 days long. In the case of exceeding the work, it was found that the spawning rate decreases relatively rapidly, and in case of inducing the spawning for more than 100 days, it is found that it is ineffective compared to the scattering effect. Accordingly, an economically suitable time is to induce spawning for up to 90 to 100 days under the spawning induction condition, thereby enabling mass production in a short period of time and stably planning production. At the same time, it can be easily used by farms, and it is expected that labor efficiency can be increased.

Hereinafter, the present invention will be described in more detail through examples. These examples are only for illustrating the present invention, it will be apparent to those skilled in the art that the scope of the present invention is not to be construed as limited by these examples.

<Example 1> Characterization of Gwangju Period suitable for improving the scattering characteristics of the American king-fly

1. Experimental method

The scattering characteristics (cumulative spawning rate and mortality rate) of the Amekira kingfish were checked for various Gwangju periods.

1) Experimental insects: Rural Development Administration, American King 12G

2) Sex ratio: Cancer: Male = 1: 2 (Female = 20, Male = 40), Mating begins immediately after fable

2) Experiment temperature: 9L / 15D, 12L / 12D, 15L / 9D (multi room incubator / DS-14MCLHP)

3) Breeding conditions: Humidity-65% RH, Illuminance-1330 ~ 1800lux, Temperature-30 ℃

4) Statistical analysis: welch ’s ANOVA test

2. Experimental results

(1) Confirmation of the average number of spawning, the cumulative spawning rate and mortality rate of 1 female per spawning day of the Gwangju period by the Gwangju period (Figures 1 and 2 and Table 1 below)

Average number of eggs laid per female per day of spawning by the Gwangju period Spawning day Average number of eggs per Gwangju 9L / 15D 12L / 12D 15L / 9D Day 20 16.8 ± 16.5a 7.5 ± 10.7b 23.2 ± 20.8a Statistical Analysis: F (2,12.125) = 3.649, p = 0.057 Day 40 7.3 ± 9.8 0.3 ± 0.8 10.7 ± 20.4 Statistical Analysis: F (2,24) = 8.146, p = 0.002 Day 62 26.8 ± 17.2ab 8.2 ± 10.9b 33.1 ± 17.9a Statistical Analysis: F (2,23) = 4.484, p = 0.023 Day 79 16.2 ± 11.4 4.0 ± 0.8 19.7 ± 17.6 Statistical Analysis: F (2,10.837) = 8.094, p = 0.007 Day 100 9.8 ± 15.2 1.7 ± 2.9 9.6 ± 7.8 Statistical Analysis: F (2,12) = 0.706, p = 0.513 Overall average 16.8 ± 16.5a 7.5 ± 10.7b 23.2 ± 20.8a Statistical Analysis: F (2,455.4) = 60.174, p = 0.0001

As shown in Table 1, the average number of eggs laid for each female spawning day by Gwangju period was confirmed that the number of eggs spawned was significantly higher at 9L / 15D or 15L / 9D, and among them, the number of eggs spawned at 15L / 9D was the most. It was confirmed that the total cumulative scattering number was the highest in 15L / 9D. On the other hand, it was confirmed that the number of spawning drops rapidly under 12L / 12D photoperiod conditions. It was predicted that a high spawning rate could be obtained when inducing the spawning of the king and the king.

However, under the conditions of 9L / 15D to 11L / 12D, as shown in FIG. 2, the cumulative mortality rate was high, especially in the case of males, in the 9L / 15D photoperiod, the relative mortality rate was higher than that of the entire male, without discrimination between male and female. In order to stably lower the mortality rate and increase the spawning rate, it is suitable to induce spawning of adult adults in 13L / 11D to 15L / 9D photoperiod conditions, and more preferably, scatter under 14L / 10D to 15L / 9D photoperiod conditions. It was found that inducing is most suitable.

In other words, in order to improve the spawning characteristics of the adult mealworms, the photoperiod has a great effect, and when the conditions of the photoperiods are not suitable for spawning, it is difficult to survive the adult life of the adult mealworms. It can be seen that identifying the spawning characteristics as described above is an essential part for increasing labor efficiency while minimizing economic loss.

<Example 2> Characterization of temperature suitable for improving the scattering properties of the American king meal

1. Experimental method

The scattering characteristics (egg rate, mortality rate) of the Amekira kingfish were checked at various temperatures.

1) Experimental insects: 11G Native American Pedigree, Rural Development Administration

2) Sex ratio: Cancer: Male = 1: 2 (Female = 15, Male = 30), Mating begins immediately after fable

2) Experiment temperature: 25 ℃, 27 ℃, 30 ℃, 33 ℃ (multi room incubator / DS-14MCLHP)

3) Breeding conditions: Humidity-65% RH, Illuminance-1330 ~ 1800lux, Gwangjugi-9L / 15D

4) Statistical analysis: welch ’s ANOVA test

2. Experimental results

(1) Confirmation of cumulative number of eggs and mortality rate of 1 female per spawning day of the American king by temperature (Fig. 3 and Table 2 below)

Cumulative number of eggs laid by one female per day of spawning by the temperature Spawning day Average number of scattering by temperature 25 ℃ 27 ℃ 30 ℃ 33 ℃ Day 32 43.1 ± 31.7b 81.1 ± 59.5ab 119.3 ± 83.2a 42.3 ± 45.0b Statistical Analysis: F (3,29.619) = 4.834, p = 0.007 Day 60 203.0 ± 89.2bc 305.4 ± 219.4ab 430.7 ± 210.2a 78.7 ± 60.4c Statistical Analysis: F (3,26.363) = 19.419, p = 0.0001 Day 70 276.3 ± 128.2ab 395.1 ± 295.6a 525.3 ± 268.9a 84.7 ± 65.9b Statistical Analysis: F (3,27.239) = 21.867, p = 0.0001 Day 80 335.9 ± 164.8b 481.5 ± 375.5ab 594.7 ± 323.6a 90.7 ± 74.1c Statistical Analysis: F (3,26.363) = 19.419, p = 0.0001 Day 91 383.9 ± 205.5ab 536.9 ± 416.1a 651.3 ± 385.1a 91.7 ± 76.6b Statistical Analysis: F (3,25.973) = 21.050, p = 0.0001 Day 120 472.0 ± 306.9a 579.1 ± 459.2a 700.9 ± 441.4a 91.7 ± 76.6b Statistical Analysis: F (2,24.989) = 19.443, p = 0.0001

As shown in FIG. 3, as a result of examining the average number of spawns per day of spawning of the American king-fly at four temperatures of 25 ° C, 27 ° C, 30 ° C, and 33 ° C, 30 ° C was the most with 27.3 ± 27.1, followed by 27 ° C. The order was 23.5 ± 27.1 at 25 ℃, 19.7 ± 19.6 at 25 ℃, and 5.2 ± 9.7 at 33 ℃, 34.1 ± 22.0 at 30 ℃, 43.1 ± 29.5 at 60 days of spawning, and 17.5 ± 16.5 at 91 days at spawning. , Most spawning was found at 4.8 ± 6.9 on the 120th day of spawning, and high significance was confirmed until 60 days of spawning. Overall, the number of spawning tended to decrease after 90 days.

As shown in Table 2 and FIG. 4, as a result of examining the average cumulative number of eggs of one American king by temperature, 30 ℃ was the most with 700.9 ± 441.1, followed by 27 ℃ 579.1 ± 459.2 and 25 ℃ 472.0 ± 306.9 , It was 461.0 ± 415.8 in order of 33 ℃, and it was confirmed that the cumulative number of eggs was not significantly changed after 90 days.

In other words, it can be seen that the proper spawning temperature of the American king-fly is 27 ~ 30 ℃, and it is found that it is most appropriate to induce spawning for up to 90-100 days.

In addition, as shown in Figure 5, by temperature (25 ℃, 27 ℃, 30 ℃, 33 ℃) as a result of investigating the life of the male and female male and female adults, the average life span of females is higher than other breeding temperatures at 27 ~ 30 ℃ On the other hand, in the case of males, it was confirmed that the higher the temperature, the lower the average lifespan. In particular, as shown in FIG. 6, the mortality rate of females increases rapidly after 100 days of spawning at all temperatures, and especially at 33 ° C, the mortality rate of females is high even before 100 days, and the spawning induction according to the death of female adults rapidly decreases. Can predict.

Accordingly, in order to obtain a high spawning rate for the longest life span without distinction between male and female, both male and female adults are incubated by inducing spawning at a temperature of 27 to 30 ° C, more preferably 28 to 29 ° C, for a maximum of 90 to 100 days. It can be seen that the mortality rate of male and female adults can be lowered by minimizing, and at the same time, it is possible to obtain a high spawning rate, which is the most suitable condition for economical spawning.

<Example 3> Identification of spawning land suitable for improving the spawning characteristics of the Great American pedigree

1. Experimental method

The spawning characteristics (by type of spawning ground) of the Amekira kingfish were checked at various temperatures.

1) Experimental insects: 11G Native American Pedigree, Rural Development Administration

2) Sex ratio: Cancer: Male = 1: 2 (Female = 15, Male = 30), Mating begins immediately after fable

2) Experiment temperature: 25 ℃, 27 ℃, 30 ℃, 33 ℃ (multi room incubator / DS-14MCLHP)

3) Breeding conditions: Humidity-65% RH, Illuminance-1330 ~ 1800lux, Gwangjugi-9L / 15D

4) Statistical analysis: welch ’s ANOVA test

2. Experimental results

As a result of the above experiment, it was confirmed that the spawning ratio of the American king-fly was different according to the type of spawning site according to the temperature.

In other words, as shown in FIG. 7 at 27 to 30 ° C., which was identified as an appropriate temperature for improving the scattering properties in Example 2, the spawning ratio was high because the spawning was well induced in the general spawning ground (paper) until 35 days of spawning. On the other hand, it was found that from 35 days to 100 days, which was identified as the longest spawning induction period in Example 2, it was very suitable to increase the spawning rate by inducing spawning in wheat bran rather than the general spawning ground (paper).

Therefore, between 35 and 40 days of spawning, bran is gradually added to 100% of the general spawning ground and replaced with 70-80% bran-coated spawning ground to induce spawning of adult adults, which increases spawning rate. Is the best fit.

Summarizing the scattering characteristics of the American pedigree investigated in this way, the American pedigree induces spawning under 13L / 11D ~ 15L / 9D photoperiod conditions and 27 ~ 30 ℃ temperature conditions, and the adult spawning period is the longest. It is suitable for 90 ~ 100 days. At this time, it is recommended that the spawning ground be raised from the general spawning ground until the 35th day of spawning, and that after 35 days, wheat bran is gradually added to 70-80% wheat bran coated to spawn to induce spawning. It can be seen that it is possible to obtain 500 to 650 eggs by 90 to 100 days, while increasing the survival rate of 60 to 80% of adult and male spawning without increasing the number of eggs.

Based on the above results, mass production is possible in a short period of time as the optimum spawning conditions of the American kingfish are identified, and stable planned production is possible. It can be expected to increase labor efficiency.

The present invention has been described with reference to preferred embodiments, and those of ordinary skill in the art to which the present invention pertains may implement embodiments in different forms from the detailed description of the present invention within the essential technical scope of the present invention. Will be able to. Here, the essential technical scope of the present invention is indicated in the claims, and all differences within the equivalent range should be interpreted as being included in the present invention.

Claims (7)

As a method of artificial spawning of the American Great Wing under the general spawning ground,
The method includes: (a) photoperiod 13L / 11D to 15L / 9D; And (b) inducing artificial spawning of the Great American kingfish under conditions of a temperature of 27 ° C to 30 ° C;
The American king meal is characterized by inducing artificial spawning by applying 70 to 80% of the general spawning ground with bran from more than 35 days of spawning.
The method of artificial spawning of the American Great Dipper.
delete According to claim 1,
The American king meal is characterized by inducing spawning in the photoperiod of 14L / 10D to 15L / 9D,
The method of artificial spawning of the American Great Dipper.
According to claim 1,
The American king meal is characterized by inducing spawning at a temperature of 29 ~ 30 ℃ from 60 to 90 days of spawning,
The method of artificial spawning of the American Great Dipper.
According to claim 1,
The American king meal is characterized by inducing spawning under conditions of 70 to 80% humidity and 1,330 to 1800 Lux illuminance.
The method of artificial spawning of the American Great Dipper.
According to any one of claims 1, 3 to 5,
The adult mealworm of the Americas is characterized in that the average spawning number is 20 to 30 per day from the 25th day to the 90th day.
The method of artificial spawning of the American Great Dipper.
According to any one of claims 1, 3 to 5,
The American king-fly adult is characterized by laying 500-650 eggs for 90-100 days.
The method of artificial spawning of the American Great Dipper.

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