KR102113849B1 - Method for artificial raise of Zophobas atratus Fabricius - Google Patents

Abstract

The present invention relates to a method of artificial breeding of the American king-fly.
The artificial breeding method of the American king meal of the present invention includes (a) breeding temperature of 27 ° C to 30 ° C, (b) photoperiod 9L / 15D to 12L / 12D, (C) King of America under conditions of 65% to 80% humidity. It is characterized by artificial breeding.
According to the present invention, mass production is possible as the optimum breeding conditions of the American kingfish are identified, and stable planned production is possible, so that the insect industry can be activated and the income increase effect can be easily used at the farm. Therefore, it is expected to increase labor efficiency.

Description

Method for artificial raise of Zophobas atratus Fabricius}

The present invention relates to an artificial breeding method for a king-of-the-american kingfish, and more specifically, to improve a breeding characteristic of a king-of-american kingfish by controlling a specific temperature, photoperiod, humidity, etc. It is about the artificial breeding method of a jerk.

Insects are one of the important roles in the natural ecosystem, which are used as animal feed, because many insect species are nutritionally rich. In Korea, it has been used as a feed for pets for more than 10 years, and has started breeding in insect lovers and zoos. Recently, as a feed insect, a number of farms are growing for industrialization due to various advantages.

In particular, the insect used for animal feed, which is widely used in the past, is the insect of the family Pegasus ( Tenebrionidae ). Several studies have been conducted on the growth characteristics, artificial breeding methods, and artificial feed of these insects.

Related prior arts include Korean Registered Patent No. 10-1660379 (How to shorten the development of brown larvae larvae), Korean Patent Publication No. 10-2017-0052128 (Feed composition for breeding larvae larvae), Korean Registered Patent No. 10-1663202 (Insect mass breeding equipment and facilities) and the like are known.

As described above, although several studies have been conducted on the beetle and insects, most of them mention only the developmental characteristics of the larvae or adult of the brownish beetle, and the American king beetle is only partially known for the normal breeding temperature. Accurate research has not yet been conducted on the optimal breeding conditions that can enhance the growth characteristics of only a jerk.

The object of the present invention is to improve the growth characteristics of the American king meal, shorten the period of development and at the same time increase the weight and lower the mortality by identifying optimal breeding conditions that can be easily used at the farm while mass-producing the American king meal. It is to provide a method of artificial breeding.

To achieve the above object, the artificial breeding method of the American king meal of the present invention includes (a) breeding temperature of 27 ° C to 30 ° C, (b) photoperiod 9L / 15D to 12L / 12D, (C) humidity of 65% to It is characterized by artificial breeding of the American king's fern under 80% of conditions.

When the above breeding conditions are applied, the period of development of the larvae of the American king-fly larvae up to 15 years is 75-85 days, and the development period of up to 18 years is 110-120 days, and conversion between 15-18 years It is made, and is characterized by showing a survival rate of 70-90%.

The technical problems to be achieved by the present invention are not limited to the technical problems mentioned above.

According to the present invention, mass production is possible as the optimum breeding conditions of the American kingfish are identified, and planned production is possible stably, so that the insect industry can be activated and the income increase effect can be easily used at the farm. Therefore, it is expected to increase labor efficiency.

1 is a graph showing the survival rate of the American king by temperature.
Figure 2 is a graph showing the mortality rate of the American king by temperature.
Figure 3 is a graph showing the development period according to the age of the American king meal according to temperature.
Figure 4 is a graph showing the change in weight according to the age of the American king meal according to temperature.
Figure 5 is a graph showing the change in the width of the age of the American king meal according to temperature.
6 is a graph showing the change in body width according to the age of the American king meal according to temperature.
7 is a graph showing the change in body length according to the age of the American king meal according to temperature.
8 is a graph showing the development period by age of caterpillars of the American king by Gwangju period.
FIG. 9 is a graph showing the weight of each larva of the American king by Gwangju period.
10 is a graph showing the width of each larvae of the American king and larvae by Gwangju period.
Figure 11 is a graph showing the body width of the larvae of the American king and larvae by Gwangju period.
12 is a graph showing the length of each larvae of the American king and larvae by Gwangju period.
13 is a graph showing the exclusive period of the king of America by Gwangju period.
14 is a graph showing the cumulative development period of each larvae of the American king and larvae by humidity.
15 is a graph showing the change in weight by age of caterpillars of the American king by humidity.

Hereinafter, the present invention will be described in detail, and detailed descriptions of well-known functions and configurations that may unnecessarily obscure the subject matter of the present invention will be omitted.

The present invention, in order to achieve the above technical problem, as a result of several studies to find the optimal breeding conditions of the American king meal, (a) breeding temperature 27 ℃ to 30 ℃, (b) Gwangjugi 9L / 15D to 12L / 12D , (C) It is found that artificial breeding of the American king's jersey under conditions of humidity of 65% to 80% shortens the development period of the American king's jersey while lowering the mortality rate and increasing the increase rate.

Hereinafter, the artificial breeding method of the American king pedigree of the present invention will be described in detail as follows.

All insects are markedly affected by conditions such as temperature, photoperiod, and humidity, which are applied during artificial breeding, and have a marked effect on the growth status or breeding status.

Thus, in the present invention, in order to shorten the period of development, and to increase the mortality rate and increase the survival rate when breeding the Great King of America, (a) breeding temperature of 27 ° C to 30 ° C, (b) photoperiod 9L / 15D to 12L / 12D, (C) breeding in an environment with a humidity of 65% to 80%. If these conditions are exceeded, the breeding rate decreases. The breeding condition is a mandatory condition that must be applied when breeding an American king. More preferably, the caterpillars of the American king-eater are (a) breeding temperature of 29 ~ 30 ℃, (b) 9L / 15D for Gwangju period 1 ~ 5, 12L / 12D after 6 years of age (c) 70 ~ It is best to breed under conditions of 80% humidity and 1,330 ~ 1,800 lux illuminance.

Particularly, when the breeding temperature exceeds 30 ° C, the mortality rate is considerably high, and when it is less than 27 ° C, the development period is relatively high, and when the photoperiod is less than 9L / 15D, the light is too high. There is a problem in that the insect itself is not suitable for growth because it is less radiated, and if it exceeds 12L / 12D, the mortality rate is high. In addition, when the humidity is applied to less than 65%, the humidity is too low, resulting in a high mortality rate, a relatively long development period, and when it exceeds 80%, the humidity is too high to be suitable for insects to grow. It becomes difficult to achieve the desired effect of the invention.

As a result of confirming the total rearing period and the rearing rate of the reared American pedigree, the period of development of the larvae of the American pedigree is 75 to 85 days, and the development period to 18 is 110 ~ It is 120 days, and shows a survival rate of 70 to 90%, so you can get a high breeding rate in a short time. In particular, there is no big difference between males and females, and breeding is possible without distinction between males and females. As the larvae have excellent breeding characteristics, it is possible to determine the breeding time, and it is possible to supply materials smoothly as demand increases, keeping in mind that insects are frequently used as material for animal feed. As the efficiency can be expected to increase, it is thought that the commercial use value will be high.

Hereinafter, the present invention will be described in more detail through examples. These examples are only for illustrating the present invention, it will be apparent to those skilled in the art that the scope of the present invention is not to be construed as limited by these examples.

<Example 1> Characterization of temperature suitable for improving the developmental characteristics of the American king meal

1. Experimental method

The growth characteristics (development period, mortality rate, weight gain) of the larvae of the Amekira kingfish at various temperatures were checked.

1) Experimental insect: King of America, Rural Development Administration, 1st larva on day 1

2) Experiment temperature: 25 ℃, 27 ℃, 30 ℃, 33 ℃ (multi room incubator / DS-14MCLHP)

3) Investigation method and period: 3 repetitions of 50 animals per experimental area (15 rapid growth stages per experimental area are extracted and the results are recorded)

4) Breeding conditions: Humidity-65%, Illuminance-1330 ~ 1800lux, Gwangjugi-9L / 15D (This is a general insect breeding condition)

2. Experimental results

(1) Confirmation of the growth period and mortality rate of American king meal by temperature (Figures 1 and 2 and Tables 1 and 2 below)

<Development period by stage of the larva of the American Great Wing according to temperature> Temperature
(℃) Larval development period (days) 1-5 orders 1-10 years old 1 ~ 15 orders 1 ~ 18 orders 25 19.6 ± 1.5 57.8 ± 2.9 107.5 ± 4.6 156.7 ± 7.5 27 16.1 ± 0.6 43.6 ± 2.4 85.5 ± 4.8 136.5 ± 9.2 30 16.2 ± 1.4 40.1 ± 2.5 75.9 ± 4.1 120.0 ± 5.8 33 13.8 ± 1.3 39.2 ± 3.0 79.3 ± 6.4 132.6 ± 10.7 * Statistical analysis: oneway ANOVA test
Age 1-5: F (3,176) = 160.222, p = 0.0001
Age 1-10: F (3,176) = 448.830, p = 0.0001
Age 1-15: F (3,89) = 228.224, p = 0.0001
Age 1-18: F (3,33) = 25.718, p = 0.0001

<The mortality rate of larvae of American kings according to temperature> Temperature
(℃) Mortality rate (%) 1-10 years old 11-21 years old Total larva period Exclusive period Pupae period 25 32.0 ± 9.6 2.7 ± 1.2b 32.7 ± 8.9b 0.0 ± 0.0 0.0 ± 0.0 27 30.1 ± 3.5 0.0 ± 0.0b 30.8 ± 2.8b 0.0 ± 0.0 0.7 ± 1.7 30 25.7 ± 8.0 1.3 ± 2.3b 27.1 ± 9.8b 2.1 ± 3.6 1.4 ± 1.2 33 41.5 ± 10.1 47.6 ± 11.4a 89.2 ± 4.1a 1.4 ± 1.2 2.0 ± 2.0 * Statistical analysis: oneway ANOVA test
Age 1-10: F (3,8) = 1.946, p = 0.201
Age 11-21: F (3,8) = 47.011, p = 0.0001
Total larval period: F (3,8) = 52.918, p = 0.0001
Exclusive period: F (3,8) = 0.898, p = 0.483
Pupa period: F (3,8) = 1.301, p = 0.339

As shown in Table 1, as a result of investigating the development period of the American king jersey at four temperatures such as 25 ° C, 27 ° C, 30 ° C, and 33 ° C, 33 ° C was the shortest at 39.2 ± 3.0 days until 1-10 years old, followed by 30 ° C 40.1 ± 2.5 days, 27 ° C 43.6 ± 2.4 days, and 25 ° C 57.8 ± 2.9 days in order, but until 15 and 18 years old, 30 ° C was 75.9 ± 4.1 days and 120.0 ± 5.8, respectively, which was the shortest among the four temperatures. In addition, when compared to 15, 30 ℃ was 4 days than 33 ℃, 10 days than 27 ℃, shortened to 32 days than 25 ℃, it was confirmed that the development period by temperature is highly significant.

In addition, as shown in Figure 1, 2 and Table 2, as a result of examining the mortality rate of the American king by temperature, mortality generally occurred within 1 to 5 years, and 33 ° C was almost 90% mortality during the larval period by temperature. On the other hand, it was confirmed that there was no difference in mortality at 25 ° C, 27 ° C, and 30 ° C.

(2) Confirmation of the growth period and weight gain by age of American king meal according to temperature (FIGS. 3 to 7)

As shown in FIG. 3, it was confirmed that the growth period was relatively short at 30 ° C between 1 and 15 years of age, while the growth period was shortest at 27 ° C after 15 years of age.

In FIGS. 4 to 7, 30 ° C was the heaviest, followed by 27 ° C, 33 ° C, and 25 ° C in order to investigate the weight of each American larvae by temperature, followed by 27 ° C, followed by high significance between temperatures. The length of the body showed the same tendency as the weight, and it was found that breeding the American king at 27 ~ 30 ℃ is most suitable for improving the growth characteristics.

(3) Confirmation of the transition period and the transition period of the American king meal according to temperature (Tables 3 and 4 below)

<The time for exclusive use of the American king according to temperature> Temperature
(℃) Exclusive period (%) 15 orders (n) 16 orders (n) 17 Commands (n) 18 (n) 19th order (n) 20 orders (n) 25 0.0 (0) 3.0 (3) 42.6 (43) 38.6 (39) 12.9 (13) 3.0 (3) 27 1.2 (1) 22.4 (19) 51.7 (44) 23.5 (20) 1.2 (1) 0.0 (0) 30 7.7 (5) 27.7 (18) 36.4 (23) 23.1 (15) 4.6 (3) 1.5 (1) 33 0.0 (0) 6.2 (1) 31.3 (5) 43.8 (7) 12.5 (2) 6.2 (1) * Statistical analysis: chi-square test: x2 = 86.973, df = 15, p = 0.0001

<Period for exclusive use of American meals depending on temperature> Temperature
(℃) Exclusive period (days) 15 Command
(n) 16 Commands
(n) 17 Command
(n) 18 Command
(n) 19 Command
(n) 20 Command
(n) 25 -
24.0 ± 1.4a
(2) 23.8 ± 3.6a
(30) 22.8 ± 2.3a
(34) 21.7 ± 1.9
(9) 23.0 ± 0.0
(One) 27 17.0 ± 0.0
(One) 20.0 ± 2.6b
(14) 18.5 ± 1.5b
(37) 18.3 ± 1.6b
(9) 20.0 ± 0.0
(One) -
30 19.0 ± 2.1
(5) 18.5 ± 1.9b
(16) 19.0 ± 2.6b
(19) 18.7 ± 2.6b
(14) 16.0 ± 0.0
(One) 22.0 ± 0.0
(One) 33 -
- 24.0 ± 0.0
(One) 22.8 ± 2.8a
(4) -
-
* Statistical analysis: oneway ANOVA test
-> Exclusive period by temperature
: Total F (3,195) = 49.552, p = 0.0001
16th: F (2,29) = 6.127, p = 0.006, 17th: F (2,83) = 26.082, p = 0.0001,
Age 18: F (3,57) = 15.741, p = 0.0001
-> Exclusive period by age
: Total F (5,193) = 1.789, p = 0.117
25 ° C F (3,71) = 1.239, p = 0.302; 27 ℃ F (4,57) = 2.317, p = 0.068
30 ° C F (5,50) = 0.727, p = 0.605; 33 ℃ F (1,3) = 0.165, p = 0.712

As a result of investigating the timing of conversion of the American kingfish by temperature (25 ℃, 27 ℃, 30 ℃, 33 ℃), as shown in Tables 3 and 4 above, the conversion is between 15 and 18 ages within the range of 27 ℃ to 30 ℃. It was confirmed that at a temperature of 25 ° C and 33 ° C, the time for conversion was relatively slower than that at 27 ° C to 30 ° C, indicating that the conversion period tended to be longer.

(4) Confirmation of the exclusive period and the pupal period for male and female females by the temperature (Table 5 below)

<Priority period and male pupae period by male and female according to temperature> Temperature
(℃) Exclusive period (days) Pupa period (days) Arm (n) Number (n) Arm (n) Number (n) 25 22.8 ± 2.3a (41) 23.4 ± 3.4a (53) 13.9 ± 1.2a (42) 14.2 ± 1.2a (55) 27 18.7 ± 1.7b (38) 19.0 ± 2.1b (42) 10.6 ± 1.1b (50) 10.9 ± 1.3b (49) 30 18.7 ± 3.0b (26) 19.1 ± 2.4b (32) 9.5 ± 0.8c (44) 9.3 ± 1.1c (44) 33 21.8 ± 3.0a (4) 25.7 ± 2.1a (3) 8.2 ± 0.8d (5) 8.5 ± 1.7c (4) * Statistical Analysis:
-> Exclusive period
oneway ANOVA test: Comparison of total male and female exclusive period: F (3,235) = 54.063, p = 0.0001
Female-only period by temperature: F (3,105) = 26.848, p = 0.0001
Male-only period by temperature: F (3,126) = 28.935, p = 0.0001
T-test: Comparison of total male and female exclusive period: t (237) =-1.387, p = 0.167
25 ° C: t (92) =-0.933, p = 0.353. 27 ° C: t (78) =-0628, p = 0.532
30 ° C: t (56) =-0.575, p = 0.568. 33 ° C: t (5) =-1.927, p = 0.112
Correlation analysis of dedicated period by temperature: R = -0.378, p = 0.0001

-> Pupa period
oneway ANOVA test: Comparison of all male and female pupal periods: F (3,289) = 287.193, p = 0.0001
Female pupal period by temperature: F (3,137) = 144.755, p = 0.0001
Male exclusive period by temperature: F (3,148) = 142.364, p = 0.0001
T-test: Total male and female pupal period comparison: t (291) =-1.600, p = 0.111
25 ° C: t (95) =-1.036, p = 0.303. 27 ° C: t (97) =-1.443, p = 0.152
30 ° C: t (86) = 0.746, p = 0.458. 33 ° C: t (7) =-0.344, p = 0.741
Correlation analysis of pupa period by temperature: R = -0.802, p = 0.0001

As a result of examining the conversion period and pupal period of male and female females by temperature, as shown in Table 5 above, within the range of 25 ℃ ~ 33 ℃, the female conversion period is 20.3 ± 3.0 days, and the male conversion period is 20.9 ± 3.5 days. There was no difference, but tended to be shorter as the temperature increased. In the case of pupal period, within the range of 25 ℃ -33 ℃, there was no difference between male and female, 11.1 ± 2.2 days for females and 11.6 ± 2.4 days for males.

Based on the above results, it can be seen that the American king larva larva has little mortality, so that the survival rate is high and the development period is shortened. It was found that the period of transfer and pupae was 27 ~ 30 ℃ as the proper breeding temperature condition, regardless of sex.

<Example 2> Characterization of the Gwangju period suitable for improving the developmental characteristics of the American king meal

1. Experimental method

The growth characteristics (development period, mortality rate, weight gain) of the larvae of the Amekira kingfish by various Gwangju were checked.

1) Experimental insect: King of America, Rural Development Administration, 1st larva on day 1

2) Experimental photoperiod: 9L / 15D, 12L / 12D, 15L / 9D (multi room incubator / DS-14MCLHP)

3) Research method and period: 3 repetitions of 30 animals per experiment

4) Breeding conditions: Humidity-65%, Temperature-30 ℃, Illuminance-1,330 ~ 1800 lux

2. Experimental results

(1) Confirmation of the growth period and mortality rate of the American king meal by Gwangju period (Figure 8 and Tables 6 and 7 below)

<Development period of each stage of the American larvae according to the light cycle> Gwangju Period (n) Larval development period (days) 1-5 orders 6-10 years old 1-10 years old 1 ~ 15 orders 9L / 15D (19) 19.2 ± 1.0b 32.3 ± 2.7b 46.9 ± 2.8b 82.6 ± 3.1 12L / 12D (20) 21.0 ± 1.1a 31.0 ± 2.0b 46.8 ± 2.1b 79.2 ± 2.6 15L / 9D (13) 21.8 ± 1.2a 34.7 ± 2.5a 50.9 ± 2.6a 84.2 ± 2.3 * Statistical analysis: oneway ANOVA test
Age 1-5: F (2,49) = 23.724, p = 0.0001
Age 6-10: F (2,49) = 9.605, p = 0.0001
Age 1-10: F (2,49) = 13.108, p = 0.0001
Age 1-15: F (2,37) = 9.814, p = 0.0001

<The mortality rate of larvae of American kings according to the light cycle> Gwangju Period Mortality rate (%) 2 orders (n) 4 command (n)) 5 orders (n) 20 orders (n) 1 to 21 orders (n) 9L / 15D 23.3 (7) 3.3 (1) 0.0 (0) 3.3 (1) 40.0 (12) 12L / 12D 26.7 (8) 3.3 (1) 0.0 (0) 0.0 (0) 46.7 (14) 15L / 9D 36.7 (11) 13.3 (4) 3.3 (1) 0.0 (0) 56.7 (17) * Statistical analysis: Chi-Square test, X ² = 13.615, df = 14, p = 0.479

As shown in Table 6 and Figure 8, 9L / 15D, 12L / 12D, 15L / 9D As a result of examining the development period of the larva under three light conditions, the 12L / 12D sphere from 1 to 15 years of age was 79.2 ± 2.6 days, 9L / 15D The 82.6 ± 3.1 day of the sphere and the shortening period of 3.4 days and 5 days from the 84.2 ± 2.3 days of the 15L / 9D sphere were confirmed to be affected by the light conditions, and the growth period was the highest in the 9L / 15D sphere until 1-5 years old. It was confirmed that the development period was shortened most in 12L / 12D after 6 years of age.

In addition, as shown in Table 7, as a result of examining the mortality rate according to the light conditions (9L / 15D, 12L / 12D, 15L / 9D), 9L / 15D sphere 40.0%, 12L / 12D sphere 46.7%, 15L / 9D sphere 56.7% Since the mortality rate of 15L / 9D was the highest, it was found that the larvae of the American king-fly larvae were bred under the conditions of 9L / 15D ~ 12L / 12D in the Gwangju period, and the mortality rate was the lowest and the lowest.

(2) Confirmation of weight increase by age of the American king and her family according to the Gwangju period (Figs. 9 to 12)

As shown in Fig. 9, as a result of examining the weight from 1 to 10 years old by light condition (9L / 15D, 12L / 12D, 15L / 9D), the 9L / 15D and 12L / 12D spheres tended to be slightly heavier than the 15L / 9D sphere However, it was confirmed that the weight gain was the highest in 9L / 15D group until 5 years old, and the weight gain was the highest in 12L / 12D group after 6 years old.

In addition, as shown in FIGS. 10 to 12, it was confirmed that the width, body width, and body length of each light condition (9L / 15D, 12L / 12D, and 15L / 9D) showed a similar tendency to the weight gain result.

(3) Confirmation of the time and period for exclusive use of the American king meal according to the Gwangju period (Tables 8, 9 and 13 below)

<The period for exclusive use of the American king according to the light cycle> Gwangju Period Exclusive period (%) 15 orders (n) 16 orders (n) 17 Commands (n) 18 (n) 19th order (n) 20 orders (n) 9L / 15D 5.6 (1) 16.7 (3) 44.4 (8) 16.7 (3) 11.1 (1) 5.6 (1) 12L / 12D 16.7 (3) 33.3 (6) 27.8 (5) 5.6 (1) 11.1 (1) 5.6 (1) 15L / 9D 15.4 (2) 38.5 (5) 46.2 (6) 0.0 (0) 0.0 (0) 0.0 (0)

<Period for exclusive use of the King of America according to the light cycle> Gwangju Period Exclusive period (days) 15 Command
(n) 16 Commands
(n) 17 Command
(n) 18 Command
(n) 19 Command
(n) 20 Command
(n) 9L / 15D -
24.0 ± 1.4a
(2) 23.8 ± 3.6a
(30) 22.8 ± 2.3a
(34) 21.7 ± 1.9
(9) 23.0 ± 0.0
(One) 12L / 12D 17.0 ± 0.0
(One) 20.0 ± 2.6b
(14) 18.5 ± 1.5b
(37) 18.3 ± 1.6b
(9) 20.0 ± 0.0
(One) -
15L / 9D 19.0 ± 2.1
(5) 18.5 ± 1.9b
(16) 19.0 ± 2.6b
(19) 18.7 ± 2.6b
(14) 16.0 ± 0.0
(One) 22.0 ± 0.0
(One) * Statistical analysis
Age 16: oneway ANOVA test: F (2,11) = 4.970, p = 0.029
Age 17: oneway ANOVA test: F (2,15) = 2.083, p = 0.159

As a result of examining the conversion period and the conversion period of the American king by Gwangju period, as shown in Tables 8, 9 and 13 above, it was confirmed that all Gwangju periods were converted between 15 and 18 ages, and up to 18 in 9L / 15D. It was confirmed that it is exclusive, and in 12L / 12D and 15L / 9D, it is converted between 15 and 17 ages, and the period during which it is converted is also shorter. Therefore, more preferably, after 15 years of age, it can be seen that breeding in a 12L / 12D Gwangju geometry is most suitable.

(4) Confirmation of the period of breeding by the male and female of the American king and its females according to the Gwangju period, pupal period, adult male and female ratio, and pupal-fable period (Tables 10, 11, and 12 below)

<Private period and male pupae period by male and female according to light cycle> Gwangju Period Exclusive period (days) Pupa period (days) Arm (n) Number (n) Arm (n) Number (n) 9L / 15D 22.8 ± 2.3a (41) 23.4 ± 3.4a (53) 13.9 ± 1.2a (42) 14.2 ± 1.2a (55) 12L / 12D 18.7 ± 1.7b (38) 19.0 ± 2.1b (42) 10.6 ± 1.1b (50) 10.9 ± 1.3b (49) 15L / 9D 18.7 ± 3.0b (26) 19.1 ± 2.4b (32) 9.5 ± 0.8c (44) 9.3 ± 1.1c (44) * Statistical Analysis:
Exclusive period (female): oneway ANOVA test: F (2,24) = 1.815, p = 0.184
Exclusive Period (Wed): oneway ANOVA test: F (2,17) = 0.884, p = 0.431
Pupa period (female): oneway ANOVA test: F (2,24) = 4.792, p = 0.018
Pupa Period (Wed): oneway ANOVA test: F (2,17) = 0.004, p = 0.996

<The ratio of male and female Americans according to the light cycle> Gwangju Period Investigation Male and female ratio (%) female cock 9L / 15D 18 50.0 50.0 12L / 12D 16 56.3 43.8 15L / 9D 13 69.2 30.8 * Statistical Analysis:
Female: Chi-Square test, X ² = 3.234, df = 2, p = 0.198
Male: Chi-Square test, X ² = 4.528, df = 2, p = 0.104

<The pupae of the American king-fly according to the light cycle-the period of development until fable> Gwangju Period Development period before fable (days) female cock Male and female average 9L / 15D 122.6 ± 12.6 123.9 ± 13.0 123.2 ± 12.4 12L / 12D 117.6 ± 14.7 116.6 ± 14.5 117.1 ± 14.1 15L / 9D 115.6 ± 8.8 110.3 ± 3.9 113.9 ± 7.9 * Statistical Analysis:
Female: Chi-Square test, X ² = 3.234, df = 2, p = 0.198
Male: Chi-Square test, X ² = 4.528, df = 2, p = 0.104

As a result of examining the period of transition and pupae of male and female Americans by Gwangju period, as shown in Table 10 above, it was confirmed that there was no significant difference between the period of transition and sex between male and female by Gwangju period.

In addition, as shown in Tables 11 and 12 above, in the case of adult king larvae, the growth period is relatively short in the 15L / 9D section, but the male / female ratio is significantly different. In order to match the male / female ratio, 9L / 15D ~ It is suitable to breed in 12L / 12D, and it is most suitable to breed in 12L / 12D from pupae to fable stage and adult breeding to meet adult male and female ratios while shortening the development period of the pre-fable stage from the pupa of the American kingfly. Was found.

Based on the above results, it can be seen that the American larvae have the lowest mortality rate and 9L / 15D ~ 12L / 12D as appropriate conditions for the rearing period, and the growth period is short. After breeding at 15D, it was found that breeding at 12L / 12D was most appropriate from the age of 6 onwards. 12L / 12D is the most suitable condition for breeding during the period of transfer, pupal period, fable, and adult. It was found to be suitable.

<Example 3> Characterization of humidity suitable for improving the developmental characteristics of the Great White Pedigree

1. Experimental method

The developmental characteristics (development period, mortality rate, weight gain) of the larvae of the Amekira kingfish by various humidity were checked.

1) Experimental insect: King of America, Rural Development Administration, 1st larva on day 1

2) Experimental humidity: 50%, 65%, 80% (multi room incubator / DS-14MCLHP)

3) Survey method and duration: 50 animals per experiment 3 times

4) Breeding condition: Temperature-30 ℃, Illuminance: 1800lux, Photoperiod: 9L / 15D

2. Experimental results

(1) Confirmation of the development period and mortality rate of the American pedigree by humidity (Fig. 14 and Tables 13 and 14 below)

<Development period of each stage of the larva of the American king according to humidity> Humidity(%) Larval development period (days) 1-5 orders 6-10 years old 1-10 years old 50% 25.8 ± 2.7a 25.0 ± 5.1 46.8 ± 1.0a 65% 21.8 ± 2.3b 24.7 ± 6.4 44.8 ± 2.3ab 80% 21.3 ± 2.5b 23.7 ± 5.8 44.0 ± 3.2b * Statistical analysis: oneway ANOVA test, post Hoc: Tukeytest
Age 1-5: F (2,130) = 41.321, p = 0.0001
Age 6-10: F (2,72) = 0.953, p = 0.391
Age 1-10: F (2,32.179) = 7.283 p = 0.002

<Mortality rate of larvae of the American king-fly according to humidity> Humidity(%) Mortality rate (%) 2 Command 4 Command 5 Commands 6 Command 1-10 years old 50% 6% 6% 8% 2% 30% 65% 2% 2% 2% 0% 10% 80% 0% 2% 2% 0% 10% * Statistical analysis: Chi-Square test, X ² = 13.615, df = 14, p = 0.479

As shown in FIG. 14 and Tables 13 and 14, the temperature was fixed at 30 ° C., and then the cumulative development period from 3 to 50%, 65%, and 80% of humidity was investigated from 1 to 10 years of larvae. % Was the shortest at 44.0 ± 3.2 days, followed by 65% 44.8 ± 2.3 days, 50% was the longest at 46.3 ± 1.0 days, and in the case of mortality, 50% was found to be 3 times higher than 65% and 80%. , It was found that the Great Britain larvae are best suited for breeding at a humidity between 65% and 80%.

(2) Confirmation of weight increase by age of American king meal according to humidity (FIG. 15)

As shown in Figure 15, as a result of examining the body weight of 1 to 10-year-olds of the American larvae, 80% of the weights of 10-year-olds were 52.8 ± 31.0g, the heaviest, and 1.6 times higher than 65% (32.7 ± 15.5g). As it is 3.3 times heavier than 50% (16.1 ± 6.1g), as a result of 1 to 10 years of age, the breeding humidity suitable for the Great King is 65% to 80%, more preferably 80% is most suitable. Was found.

Summarizing the growth characteristics of the American king-fly that was investigated in this way, the development period of the larvae of the American king-fowl until 15 years is 75 days to 85 days, and the development period of 18 days to 110 days is 110 to 120 days. , It is characterized in that the conversion is made between 15 and 18 years of age, and it shows a survival rate of 70 to 90%, indicating a high breeding rate in a short time.

Based on the above results, mass production is possible in a short period of time as the optimal breeding conditions of the American kingfish are identified, and stable planned production is possible. It can be expected to increase labor efficiency.

The present invention has been described with reference to preferred embodiments, and those of ordinary skill in the art to which the present invention pertains may implement embodiments in different forms from the detailed description of the present invention within the essential technical scope of the present invention. Will be able to. Here, the essential technical scope of the present invention is indicated in the claims, and all differences within the equivalent range should be interpreted as being included in the present invention.

Claims (8)

(a) breeding temperature 30 ℃;
(b) 9L / 15D photoperiod for 1 to 5-year-old caterpillars and 12L / 12D photoperiod for caterpillars from 6 years of age onwards;
(c) humidity 80%; And
(d) roughness 1,330-1,800 Lux;
Artificial breeding method for shortening the period of development of the Great American Great Walled Caterpillar,
The growth period for the 15-year-old larvae of the American king larvae is 75 to 85 days, and the development period for the 18-year-old larvae is 110 to 120 days;
Characterized in that the caterpillars of the American king-fly are converted between 15 and 18 years of age,
Artificial breeding method for shortening the development period of the American Greater Larva.
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