KR101660379B1 - Method for Stimulating Rapid Growth of Tenebrio molitor Larvae - Google Patents

Abstract

The present invention relates to hatching brown goat larvae (a) breeding temperature from 25 ° C to 27.5 ° C; (b) long-day condition 14L: 10D; And (c) breeding larvae at a density of at least 5 to 8 cm2 per individual.
The method for breeding the larvae of the present invention improves the growth and shortens the breeding period. Therefore, it can be applied to mass production of larvae for animal feed or useful food production, Very useful for supply.

Description

{Method for Stimulating Rapid Growth of Tenebrio molitor Larvae}

The present invention relates to a method for shortening the development period of brown larvae by optimal breeding conditions.

Tenebrio molitor or meal worm belonging to the insect beetle beetle ( Tenebrionidae ) are known to live in relatively dry soils, under rocks, in trees, in animal shelters or in grain piles, It has a hatching period of 10 to 12 days from eggs and has a four-stage growth stage of larva, pupa, and adult passing through a process of peeling 15 to 20 times. The total growth period is relatively long as 280 to 630 days, cm. < / RTI > Brown goat larvae are recognized as insects in grains, but recent researches on various nutritional analysis have proven to be suitable as efficient animal feeds.

Brown larvae had a protein content of 45-60% by dry weight, which contained more protein (BCAA-leucine 4.5%, valine 3%, isoleucine 2.5%) essential for muscle formation than beef, 30-45% It is used for food such as reptiles, fish, small animals, birds, aquaculture, etc., which are usually kept in a pet or zoo because of the characteristics of the high protein. In general, individuals who grow insects as pets or breed themselves for feed use to save costs at the zoo. Commercially, they use hormones to maintain the larval stage, so that they grow up to 3 cm or larger. (Aquilar-Miranda, ED et al ., J Agric . Food Chem . , ≪ / RTI > 50 (1): 192,2002).

Typically, feed insect development techniques including crickets and brown goose larvae have recently become increasingly interested in livestock diseases such as mad cow disease, and as a safe protein source by the development of economical new materials that can replace fish meal, the main component of feed, Has been highlighted. In particular, the pet food insect market will continue to expand with the diversification of pet species.

Although the insect industry in Korea is currently being carried out only in some fields such as natural enemies, flower pots, and pets, the feed application field is currently in the stage where the domestic market has begun to be established, and investment in technology development is considerably inferior to that of advanced countries, It can be utilized as an opportunity for environmentally friendly, high value-added agriculture.

Accordingly, the present inventors have made extensive efforts to improve the method of shortening the development period for efficient industrial use of the brown feedworm larva, which is a typical feed insect, as a result of raising the temperature of breeding, The survival rate was improved, the larval period was shortened by about one month, and the survival rate was improved by water supply. Thus, the present invention was completed.

It is an object of the present invention to provide a method for raising brown larvae that can shorten the rearing period of freshly hatched brown goat larvae.

In order to accomplish the above object, the present invention relates to a hatching brown goat larva having (a) a breeding temperature of 25 ° C to 27.5 ° C; (b) long-day condition 14L: 10D; And (c) breeding larvae at a density of at least 5 to 8 cm2 per individual.

The method for breeding the larvae of the present invention improves the growth and shortens the breeding period. Therefore, it can be applied to mass production of larvae for animal feed or useful food production, Very useful for supply.

FIG. 1 is a graph showing the change in survival rate due to water supply and the number of brown larvae and water.
FIG. 2 is a graph showing changes in weight of brown larvae and pupa by water supply.
FIG. 3 is a photograph of a larva of brown duckworms according to water supply.

Hereinafter, the present invention will be described in detail.

The present inventors have made efforts to improve the method of shortening the development period for efficient industrial use of a representative feed insect, Brown Drosophila larvae, and found that the optimal conditions for breeding temperature, population density, light period and moisture supply , The survival rate was improved and the larval period was shortened by about one month and the survival rate was improved by water supply when the environment of group rearing and long day cycle was maintained at 25 to 27.5 ° C.

In one aspect, the present invention relates to hatching brown larvae (a) breeding temperature from 25 ° C to 27.5 ° C; (b) long-day condition 14L: 10D; And (c) breeding larvae at a density of at least 5 to 8 cm2 per individual.

The breeding method according to the present invention is characterized in that it is preferably raised at 25 캜.

The breeding method of the present invention can be applied to breeding larvae from immediately after hatching of eggs to shortly before hatching.

The 'dragon' is a process in which the larva is pupae-ized, and the pupa is transformed into an adult by alienation of the pupa.

In the breeding method of the present invention, the 'group breeding' can be carried out so that the individual density in the breeding box (or the container) is at least 5 to 8 cm 2 per individual. Therefore, .

In the present invention, the feeding method further comprises feeding the moisture-retaining material three times a week.

The breeding method of the present invention basically supplies the moisture-retaining material twice a week to shorten the larval rearing period, but the survival rate of larvae is improved by water supply three times a week.

The water supply used in the breeding method of the present invention is possible by supplying a moisture retaining material to a breeding box (or a container) for breeding larvae.

The moisture-retaining material used in the breeding method according to the present invention is characterized in that it is selected from sawdust, sponge, Chinese cabbage, and cotton wool, preferably Chinese cabbage, but is not limited thereto.

In the present invention, the moisture-retaining material is characterized by containing an initial moisture content of 60% to 70% moisture.

In the method for feeding brown larvae according to the present invention, the food, the photoperiod providing method and the like which are not specified are characterized by employing a technique which can be easily selected by those skilled in the art.

The method for raising a brown goat larva according to the present invention is a method for breeding larvae by maintaining a specific breeding condition. Specifically, for example, eggs are harvested from brown goat's eggs, hatching larvae are obtained from the eggs, (A) Breeding temperature 25 ° C to 27.5 ° C; (b) long-day condition 14L: 10D; And (c) by raising the larvae at a density of 5 to 8 cm 2 per at least one individual, the larval growth can be promoted to shorten the breeding period, and furthermore, the survival rate can be improved by supplying the moisture- have.

The present invention will be described in more detail with reference to the following examples. However, the following examples are provided to aid understanding of the present invention, and the scope of the present invention is not limited by these examples in any sense.

In this case, unless otherwise defined, technical terms and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. In the following description and the accompanying drawings, A description of known functions and configurations that may unnecessarily obscure the description of the present invention will be omitted.

Example  One: Brown  breed

The brown duck adults (about 1,000) were bred on bottoms at a height of about 0.8 cm, which was fed to an acrylic box with a width of 48 cm and a length of 49 cm. Moisture was supplied twice a week with various fresh vegetables (cabbage, radish, carrots, etc.), and the temperature was maintained at 25 ℃ by a temperature controller. Also, the light condition was set at 14L: 10D, which shows the highest growth rate in the breeding room.

Example  2: Depending on the type of breeding Brown ass  Difference in larval development

In order to confirm the difference in larval development period by individual breeding and group rearing, the following experiment was carried out.

In the individual breeding experiment group, 1 freshly hatched larvae were put into each of 33 dogs (6 cm in diameter, 1.5 cm in height) with 4 g of wheat bran, and 3 g of Chinese cabbage was supplied with water (28.26 cm 2 / individual ). On the other hand, in the group feeding experiment group, 10 freshly hatched larvae were put into each 15 g of bran-fed petridish (diameter 10 cm, 4 cm), and 7 g of Chinese cabbage was given twice a week to supply water 7.85 cm < 2 > / 1 object).

The experimental group was placed in an incubator (manufacturer, manufacturing country) set at 17.5 ° C, 20 ° C, 22.5 ° C, 25 ° C 27.5 ° C, or 30 ° C, The lengths were compared and observed.

As a result, the larval development period of the larvae of the group that had been raised in all the temperature conditions was shorter than that of the individual group, in the experiment that revealed the physiological characteristics between the larval group larvae and the individual breeding by temperature, The larva could not live. It was observed that the larval development period was shortened as the temperature increased from 17.5 ℃ to 25 ℃ in the grouping condition, whereas it was 129.01 days longer than the 27.5 ℃ (126.56 days) condition at 30 ℃.

Therefore, it was confirmed that 25 ° C to 27.5 ° C was the optimal temperature for larval development in the group rearing condition (Table 1).

Temperature Breeding form Larval development period
(Work) Pupa period
(Work) Pupa weight
(mg) Adult Weight
(mg) Adult body length
(mm) 30 ℃
individual 165.06 ± 15.90 6.00 ± 1.18 140 ± 0.04 120 ± 0.03 14.8 ± 0.18 group 129.01 + - 24.93 6.76 ± 3.19 180 ± 0.03 140 ± 0.03 15.6 ± 0.12 27.5 DEG C
individual 158.3 ± 11.06 6.33 ± 2.11 160 ± 0.05 130 ± 0.04 15.1 ± 0.16 group 126.56 ± 18.8 6.51 ± 1.19 170 ± 0.03 150 ± 0.03 15.6 ± 0.12 25 ℃
individual 157.35 7.85 10.20 ± 1.66 210 ± 0.03 180 ± 0.03 16.3 ± 0.09 group 135.89 ± 14.73 8.48 ± 1.58 200 ± 0.04 160 ± 0.03 16.2 + 0.14 22.5 DEG C
individual 199.00 + - 8.72 13.20 ± 1.10 160 ± 0.03 120 ± 0.03 14.6 ± 0.09 group 158.64 ± 13.91 13.88 ± 2.75 170 ± 0.02 140 ± 0.02 15.6 ± 0.10 20 ℃
individual 204.14 ± 15.35 14.00 ± 2.10 190 ± 0.03 160 ± 0.03 15.3 ± 0.15 group 168.59 ± 14.12 15.5 ± 3.79 180 ± 0.03 150 ± 0.03 16.8 ± 0.09 17.5 DEG C
individual - - - - - group 215.88 ± 16.55 20.24 ± 3.31 180 ± 0.03 160 ± 0.08 15.5 + 0.11

Example  3: According to the light period Brown ass  Larval development difference

The following experiment was carried out to confirm the optimum temperature condition in the breeding method and to confirm the difference in larval development depending on the difference of light period.

One freshly hatched larva was placed in a Petri dish (diameter 6 cm, height 1.5 cm) containing 4 g of wheat bran, and 3 g of Chinese cabbage was provided twice at an incubator at 25 ° C to feed water. At this time, larval period, longevity period, weaning weight, adult weight, adult length, and adultization rate were compared after setting the light period to 10L: 14D, 12L: 12D and 14L:

As a result, the photoperiod condition of 14L: 10D was the shortest with the larval period of 157.35 days, and it was confirmed that the above light condition was optimal for larval development (Table 2).

Guangzhou Larval period (days) Pupa weight
(mg) Pupa period
(Work) Adult rate
(%) Adult Weight
(mg) Adult body length
(mm) 25 ℃
14L: 10D
( Long-term condition ) 157.35 7.85 210 ± 0.03 10.20 ± 1.66 45.5 180 ± 0.03 16.3 ± 0.09 25 ℃
12L: 12D 184.89 + 16.76 220 ± 0.02 8.65 ± 2.40 51.5 180 ± 0.03 16.9 ± 0.09 25 ℃
10L: 14D
(Single condition) 179.56 ± 30.22 220 ± 0.06 9.75 ± 1.75 24.2 180 ± 0.04 16.4 ± 0.14

Example  4: Depending on the moisture supply Brown ass  Larval development difference

In order to determine the difference in larval development according to the optimum feeding conditions of Examples 1, 2 and 3, the experimental groups were divided into 200 litters and 190 g of bran-fed plastic containers : 36 cm, length: 27 cm, height: 8 cm), and water supply was varied by the amount of Chinese cabbage (4.86 cm 2/1 individual). The cabbage for the water supply was supplied with 23 g in consideration of the size at the initial larval stage, and increased to 50 g after one month.

 Supply of water for Chinese cabbage is divided into 1 week (Monday), twice a week (Monday, Thursday), 3 times a week (Monday, Wednesday, Friday) Survival rate, development period, weight, and dragon weight were observed once a week.

As a result, it was confirmed that the larva survived three times a week, and the weight reached 9 mg / person at 6th week. Thus, it was confirmed that the three weekly water feeding conditions were optimal for larval development Figures 1, 2 and 3).

Claims (8)

delete delete delete delete delete A method for breeding brown larvae of hatching brown larvae, characterized by the following conditions,
(a) Breeding temperature 25 캜;
(b) long-day condition 14L: 10D;
(c) a density of at least 7.85 cm2 per larva; And
(D) 60 to 70% of water, and 23 to 50 g of water-retaining material consisting of Chinese cabbage is fed three times a week to collectively breed brown larvae,
Wherein the survival rate of the larvae of brownish larvae is improved and the development period is shortened.
A method of shortening the developmental period of brown larvae.
delete delete

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