CN105875519A - Method for efficiently reproducing brachymeria lasus by taking corcyra cephalonica pupae as hosts - Google Patents
Method for efficiently reproducing brachymeria lasus by taking corcyra cephalonica pupae as hosts Download PDFInfo
- Publication number
- CN105875519A CN105875519A CN201610402099.7A CN201610402099A CN105875519A CN 105875519 A CN105875519 A CN 105875519A CN 201610402099 A CN201610402099 A CN 201610402099A CN 105875519 A CN105875519 A CN 105875519A
- Authority
- CN
- China
- Prior art keywords
- cocoon
- rice moth
- lower limb
- band
- pupa
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 title claims abstract description 22
- 241001033777 Brachymeria lasus Species 0.000 title abstract description 12
- 241000993412 Corcyra cephalonica Species 0.000 title abstract 4
- 241000753145 Sitotroga cerealella Species 0.000 claims description 204
- 241000382353 Pupa Species 0.000 claims description 199
- 210000003141 lower extremity Anatomy 0.000 claims description 147
- 241000317929 Chalcididae Species 0.000 claims description 141
- 241000256844 Apis mellifera Species 0.000 claims description 137
- 230000003071 parasitic effect Effects 0.000 claims description 80
- 238000004321 preservation Methods 0.000 claims description 70
- 239000007788 liquid Substances 0.000 claims description 38
- 230000001488 breeding effect Effects 0.000 claims description 25
- 238000009395 breeding Methods 0.000 claims description 23
- 240000001624 Espostoa lanata Species 0.000 claims description 20
- 235000009161 Espostoa lanata Nutrition 0.000 claims description 20
- 239000004677 Nylon Substances 0.000 claims description 19
- 229920001778 nylon Polymers 0.000 claims description 19
- 241000238631 Hexapoda Species 0.000 claims description 17
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 15
- 229930006000 Sucrose Natural products 0.000 claims description 15
- 235000013305 food Nutrition 0.000 claims description 13
- 230000018109 developmental process Effects 0.000 claims description 11
- 102000002322 Egg Proteins Human genes 0.000 claims description 10
- 108010000912 Egg Proteins Proteins 0.000 claims description 10
- 210000004681 ovum Anatomy 0.000 claims description 10
- 239000005720 sucrose Substances 0.000 claims description 10
- 230000001419 dependent effect Effects 0.000 claims description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 9
- ODHCTXKNWHHXJC-VKHMYHEASA-N 5-oxo-L-proline Chemical compound OC(=O)[C@@H]1CCC(=O)N1 ODHCTXKNWHHXJC-VKHMYHEASA-N 0.000 claims description 8
- 244000060011 Cocos nucifera Species 0.000 claims description 8
- 235000013162 Cocos nucifera Nutrition 0.000 claims description 8
- 241000607479 Yersinia pestis Species 0.000 claims description 8
- 238000007865 diluting Methods 0.000 claims description 8
- 239000012153 distilled water Substances 0.000 claims description 8
- 229940043131 pyroglutamate Drugs 0.000 claims description 8
- 230000008859 change Effects 0.000 claims description 7
- 210000000582 semen Anatomy 0.000 claims description 7
- 230000032669 eclosion Effects 0.000 claims description 6
- 239000000463 material Substances 0.000 claims description 6
- 241000255777 Lepidoptera Species 0.000 claims description 5
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 5
- 235000019789 appetite Nutrition 0.000 claims description 5
- 230000036528 appetite Effects 0.000 claims description 5
- 235000013339 cereals Nutrition 0.000 claims description 5
- 235000013312 flour Nutrition 0.000 claims description 5
- 239000004615 ingredient Substances 0.000 claims description 5
- 239000004033 plastic Substances 0.000 claims description 5
- 239000000843 powder Substances 0.000 claims description 5
- 238000007873 sieving Methods 0.000 claims description 5
- 239000012141 concentrate Substances 0.000 claims description 2
- 238000003892 spreading Methods 0.000 claims description 2
- 230000007480 spreading Effects 0.000 claims description 2
- 235000015097 nutrients Nutrition 0.000 abstract description 10
- 230000024241 parasitism Effects 0.000 abstract description 5
- 230000008901 benefit Effects 0.000 abstract description 4
- 230000029264 phototaxis Effects 0.000 abstract description 3
- 238000011081 inoculation Methods 0.000 abstract 1
- 239000010410 layer Substances 0.000 description 23
- 239000013589 supplement Substances 0.000 description 9
- 235000013601 eggs Nutrition 0.000 description 6
- 241000209094 Oryza Species 0.000 description 5
- 235000007164 Oryza sativa Nutrition 0.000 description 5
- 230000008569 process Effects 0.000 description 5
- 235000009566 rice Nutrition 0.000 description 5
- 230000000694 effects Effects 0.000 description 4
- 230000017448 oviposition Effects 0.000 description 4
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 3
- 239000008103 glucose Substances 0.000 description 3
- 230000002265 prevention Effects 0.000 description 3
- 230000001850 reproductive effect Effects 0.000 description 3
- 239000002356 single layer Substances 0.000 description 3
- 210000000689 upper leg Anatomy 0.000 description 3
- 241000008892 Cnaphalocrocis patnalis Species 0.000 description 2
- 229920000742 Cotton Polymers 0.000 description 2
- 229930091371 Fructose Natural products 0.000 description 2
- 239000005715 Fructose Substances 0.000 description 2
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 description 2
- 244000021150 Orbignya martiana Species 0.000 description 2
- 235000014643 Orbignya martiana Nutrition 0.000 description 2
- 241001622642 Parnara bada Species 0.000 description 2
- 238000004140 cleaning Methods 0.000 description 2
- 238000005520 cutting process Methods 0.000 description 2
- 230000003203 everyday effect Effects 0.000 description 2
- 241000894007 species Species 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 241000256848 Apis florea Species 0.000 description 1
- 241000257303 Hymenoptera Species 0.000 description 1
- 241000409991 Mythimna separata Species 0.000 description 1
- 244000004005 Nypa fruticans Species 0.000 description 1
- 235000005305 Nypa fruticans Nutrition 0.000 description 1
- 241000665056 Opisina arenosella Species 0.000 description 1
- 241000500437 Plutella xylostella Species 0.000 description 1
- 240000000111 Saccharum officinarum Species 0.000 description 1
- 235000007201 Saccharum officinarum Nutrition 0.000 description 1
- 241000985245 Spodoptera litura Species 0.000 description 1
- 206010000496 acne Diseases 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 230000002113 chemopreventative effect Effects 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 210000003608 fece Anatomy 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 230000000266 injurious effect Effects 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 238000009940 knitting Methods 0.000 description 1
- 238000011031 large-scale manufacturing process Methods 0.000 description 1
- 231100000518 lethal Toxicity 0.000 description 1
- 230000001665 lethal effect Effects 0.000 description 1
- 210000003205 muscle Anatomy 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000019617 pupation Effects 0.000 description 1
- 230000000384 rearing effect Effects 0.000 description 1
- 238000010025 steaming Methods 0.000 description 1
- 230000001502 supplementing effect Effects 0.000 description 1
- 239000002023 wood Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Feed For Specific Animals (AREA)
- Fodder In General (AREA)
- Jellies, Jams, And Syrups (AREA)
Abstract
The invention discloses a method for efficiently reproducing brachymeria lasus by taking corcyra cephalonica pupae as hosts. According to the method, the cocooned corcyra cephalonica pupae which are refrigerated for 1.5 h at 5 DEG C are mainly taken as substitute hosts of the brachymeria lasus, the brachymeria lasus reproducing method is easy, convenient and rapid to oeprate, and the reproducing efficiency is high. Brachymeria lasus inoculation is conducted on the upper layer of a brachymeria lasus reproducing cage through the cocooned corcyra cephalonica pupae, the phototaxis of the brachymeria lasus is effectively utilized, the distance between the brachymeria lasus and a nutrient source and the hosts is shortened, the parasitism rate of the brachymeria lasus to the hosts and the feeding frequency are increased, the brachymeria lasus can be uninterruptedly and massively reproduced, therefore, scale production of the brachymeria lasus is achieved, and the obvious ecological benefit and economic benefit are achieved.
Description
Technical field
The present invention relates to agriculture and forestry injurious insect field of biological control, relate to the rearing method of a kind of parasitic wasp, tool
Body relates to a kind of method efficiently breeding vast lower limb chalcid fly using rice moth pupa as host.
Background technology
Cortex cocois radicis is knitted moth Opisina arenosella Walker and is had another name called blackhead crawler belt worm, coconut palm moth moth, Cortex cocois radicis wood moth,
Belong to Lepidoptera Mu E section, be that one seriously causes harm Cortex cocois radicis, Semen livistonae chinensis, Middle East nipa palm, Semen Arecae, big yagua etc. 30
The Severe pests of multiple babassu.Mainly upwards cause harm from Cortex cocois radicis lower floor Lao Ye with larva, take food mesophyll,
Only remaining upper strata young leaves 3～4 time serious, rest blade is eaten up, thus causes the underproduction and quality to decline, sternly
During weight, Cortex cocois radicis gel yield reduces more than 45%.
The taller and bigger babassu such as Cortex cocois radicis is due to the more difficult enforcement of chemical prevention, and larva is at vacuum side of blade
Forming the burrow that feces interweaves with silk, harm of moving under water in burrow, mature larva is weaved silk before pupating under frass
Cocooing, pupa is positioned in frass and cocoon, and chemopreventive effects is poor.Only rely on chemical prevention, it is impossible to fundamentally
Prevent and kill off Cortex cocois radicis and knit moth, this worm can be made repeatedly to cause harm.The harm of moth knitted by Cortex cocois radicis becomes the key issue of coconut industry.
Knitting the harm of moth in order to more effectively control Cortex cocois radicis, reduce the pollution to environment, Appropriate application parasitic wasp is carried out
The prevention and control of moth knitted by Cortex cocois radicis is current first-selected measure.
At home, first applicant is found that the main parasitic honeybee of chrysalis phase knitted by Cortex cocois radicis is vast lower limb chalcid fly
Brachymeria lasus Walker.Vast lower limb chalcid fly belongs to Hymenoptera, Chalcididae, thigh Nikol.This is posted
The nest cocoon of moth knitted by raw honeybee Cortex cocois radicis of can breaking by the teeth, is knitted by ovipositing hosts and 2 kinds of lethal Cortex cocois radiciss of mode of host-feeding
Chrysalis.At 25 DEG C, vast lower limb chalcid fly Life of Adult 35.4 days, the filial generation quantity that queen bee is produced all one's life is
About 69, it is possible to parasitic and take food about 87 Cortex cocois radiciss and knit chrysalis.Domestic the most not this parasitic wasp is advised
The relevant report of modelling breeding.
Therefore, solve the artificial extensive of vast lower limb chalcid fly and raise, be realize Cortex cocois radicis knit moth, rice leaf roller,
One of effective and crucial approach of many Species of Lepidopterous Insect Pests sustainable harnessing such as rice green caterpillar, rice plant skipper.
Summary of the invention
It is an object of the invention to provide a kind of side efficiently breeding vast lower limb chalcid fly using rice moth pupa as host
Method, the method mainly using the rice moth pupa of cold preservation 1.5h band cocoon at 5 DEG C as the Hosts of vast lower limb chalcid fly,
Numerous honeybee method is simple and efficient, and reproductive efficiency is high.Carry out connecing honeybee with the rice moth pupa of band cocoon on the upper strata of numerous honeybee cage,
Effectively utilize the phototaxis of vast lower limb chalcid fly, shorten vast lower limb chalcid fly and nutrient source and the distance of host, carry
High vast lower limb chalcid fly to the parasitic rate of host and the frequency that takes food, can incessantly the vast lower limb of amount reproduction little
Honeybee, thus realize the large-scale production of vast lower limb chalcid fly, there is obvious ecological benefits and economic benefit.
To achieve these goals, the technical scheme is that offer is the most numerous using rice moth pupa as host
The method growing vast lower limb chalcid fly, comprises the following steps:
(1) Hosts is raised: spread one layer 1～1.5cm thick feedstuff in plastic casing；On feedstuff uniformly
Spill 1～1.5mL rice moth egg, more just cover the feedstuff of ovum grain with sieving to float in the air one layer；After 15 days every 3 days
Throwing a feedstuff, input amount is the larva appetite of 3 days；After larvae development to 30 day, on the surface of feedstuff
Spreading two parallel nylon ropes, rope two ends are exposed, and the when of feeding intake thereafter, by described rope embedment material
In；After larva cocoons and pupates, described rope is together mentioned together with cocoon block；Take the rice moth pupa of the upper band cocoon of rope,
Standby；
(2) prepare numerous honeybee cage: indoor numerous honeybee, numerous honeybee cage is placed in top and is provided with fluorescent tube and bottom setting
Having on the foster worm frame of wire gauze, described numerous honeybee cage is made up of nylon wire, and 2/3～3/4 height in numerous honeybee cage
Place is provided with warp layer net, and gauze is fixed on numerous honeybee cage by fixture, and gauze is formed up and down in making numerous honeybee cage
Two-layer, upper and lower two-layer communicates；
Owing to fluorescent tube is arranged at above numerous honeybee cage, the light above numerous honeybee cage is stronger, the most vast lower limb chalcid fly
Having the strongest phototaxis, above numerous honeybee cage, luring of light is gathered in cage top under collection；Simultaneously by numerous honeybee cage
It is set to upper and lower two-layer.The gauze distance away from numerous honeybee cage top in upper strata is little, about 7.5～10cm, so that
It is in the vast lower limb chalcid fly at cage top more frequently to take food and be positioned over the mixed sugar liquid on upper strata and band cocoon
Rice moth pupa supplements the nutrients, and improves the parasitic wasp parasitic rate to band cocoon rice moth pupa simultaneously；Lower floor is vast lower limb chalcid fly
Sufficient activity space is provided, significantly reduces the probability that vast lower limb chalcid fly is susceptible.
(3) the band cocoon rice moth pupa of cold preservation is obtained: the rice moth pupa of band cocoon is placed in cold preservation 1.5h at 5 DEG C, it is thus achieved that
The band cocoon rice moth pupa of cold preservation, standby；
(4) the rice moth pupa of band cocoon is obtained: on the cocoon of rice moth pupa, cut one long 2～3mm with shears,
Wide 1～the oval port of 1.5mm, make rice moth pupa partial denudation out, it is thus achieved that the rice moth pupa of band cocoon,
Standby；
(5) vast lower limb chalcid fly is gathered: knit moth at Cortex cocois radicis and cause harm peak period, by the coconut palm parasitic by vast lower limb chalcid fly
Son is knitted chrysalis and is gathered go back to indoor, collects vast lower limb chalcid fly as all sources of honeybee；
(6) vast lower limb chalcid fly is raised:
A, in numerous honeybee cage on the gauze on upper strata place the rice moth pupa of band cocoon, cold preservation band cocoon rice moth pupa with
And speckle with the cotton balls of the mixed sugar liquid of concentration 10%～25%, described mixed sugar liquid by weight 112 by sugarcane
Sugar,confectioner's glucose, adds distilled water diluting and makes；Rice moth pupa with cocoon and the band cocoon rice moth of cold preservation
Pupa specific lobe is 1 10～20；
B, putting in numerous honeybee cage by vast lower limb chalcid fly, the sex ration of the most vast lower limb chalcid fly is 13, raises close
Degree is: 8～10cm3/ head, controls the temperature 25 DEG C～27 DEG C of numerous honeybee room, and relative humidity is 65%～85%,
Lighting time is 12～15h；Vast lower limb chalcid fly takes food mixed sugar liquid and rice moth pupa；
C, every 3 days supplementary 100～200 vast lower limb chalcid flies in numerous honeybee cage, replacing in every 3 days speckles with mixed 1 time
Close the cotton balls of sugar liquid, the rice moth pupa of band cocoon and the band cocoon rice moth pupa of cold preservation；By vast lower limb chalcid fly queen bee number
Measure the honeybee worm ratio that band cocoon rice moth pupa specific lobe is 17 with cold preservation, the band cocoon rice moth pupa of cold preservation is divided and is placed in
In numerous honeybee cage in the culture dish on the gauze of upper strata, allow the rice moth pupa of vast lower limb chalcid fly nature parasitic strip cocoon；
D, will change after the rice moth pupa of band cocoon and the band cocoon rice moth pupa of cold preservation take out and put in conical flask,
Bottleneck seals up the nylon gauze of 100～200 mesh, tightens with rubber band, conical flask is positioned over room temperature 25 DEG C～
Under the conditions of 27 DEG C, the development duration of ovum to adult is 15～18 days；
E, after the little bee eclosion of vast lower limb in conical flask, take off the nylon gauze of bottleneck, put in dependent insect cage,
Being placed with the cotton balls speckling with mixed sugar liquid in dependent insect cage, dependent insect cage is provided above fluorescent tube, allows vast lower limb chalcid fly climb
Go out conical flask, concentrate on for breeding the next generation in dependent insect cage, or knit the squamas such as moth for field release preventing and treating Cortex cocois radicis
Wing mesh insect.
Further, the feed ingredient in step (1) calculates by weight by Semen Maydis powder 50 parts, flour 25
Part, 20 parts of Testa Tritici, white sugar 3 parts, 2 parts of yeast are configured to.
Further, described step (6) raises the stocking density of vast lower limb chalcid fly is 9cm3/ head.
The present invention efficiently breeds the method for vast lower limb chalcid fly and has following useful effect using rice moth pupa as host
Really:
(1) having present invention firstly discovers that can be with rice moth pupa host's biological control vast lower limb chalcid fly as an alternative.
The man-made feeds simplicity of rice moth is easy to get, and breeding technology is ripe, it is possible to achieve large-scale breeding.
(2) present invention uses the rice moth pupa host as an alternative of the band cocoon through cold preservation 1.5h at 5 DEG C, its
Breeding the effect of vast lower limb chalcid fly and natural host Cortex cocois radicis, to knit chrysalis suitable.
(3) present invention is by by parasitic through supplying vast lower limb chalcid fly at the band cocoon rice moth pupa of 5 DEG C of cold preservation 1.5h；
The parasitic rate of the band cocoon rice moth pupa through cold preservation is significantly higher than the band cocoon rice moth pupa without cold preservation by parasitic wasp
Parasitic rate, be significantly higher than the parasitic rate of the rice moth pupa to band cocoon simultaneously, eliminate time-consuming general
Rice moth pupa with cocoon supplies the tedious steps that this parasitic wasp is parasitic after cutting mouth；Simultaneously because rice moth is easily achieved greatly
Scale dependency, it is possible to obtain substantial amounts of rice moth pupa and supply vast lower limb chalcid fly parasitism, it is achieved thereby that vast lower limb is little
The large-scale breeding of honeybee.
(4) instant invention overcomes Cortex cocois radicis when vast lower limb chalcid fly breeds to knit the amount reproduction of the natural hosts such as moth and take a lot of work
Time-consuming, limited source, nature foodstuff Cortex cocois radicis leaf etc. are limited simultaneously, it is impossible to meet this parasitic wasp of biological control
Problem to the wilderness demand of host.
(5) numerous honeybee cage of the present invention is set to upper and lower two-layer, and upper strata is put rice moth pupa and speckled with 10%～25% with holding
The culture dish of the cotton balls of mixed sugar liquid；Lower floor provides the activity space of abundance for parasitic wasp, significantly reduces
The probability that vast lower limb chalcid fly is susceptible.
(6) present invention uses the rice moth pupa of band cocoon, the band cocoon rice moth pupa of cold preservation is 1 by optimal specific lobe
10～20 are applied in combination, and the rice moth pupa of band cocoon is mainly as supplying nutrient source vast lower limb chalcid fly, and 5 DEG C cold
Hide 1.5h band cocoon rice moth pupa and supply vast lower limb chalcid fly parasitism, the rice moth after two kinds of different disposal mainly as host
Pupa is applied in combination, it is ensured that this parasitic wasp of biological control.
(7) utensil that the present invention uses is very simple, only use numerous honeybee cage, culture dish, Cotton Gossypii, refrigerator,
Conical flask, dependent insect cage etc., numerous honeybee efficiency is high, has wide industrialization prospect.
Accompanying drawing explanation
Fig. 1 is that numerous honeybee cage that the present invention efficiently breeds using rice moth pupa as host in the method for vast lower limb chalcid fly is put
Structural representation on foster worm frame；
In figure: 1, fluorescent tube；2, worm frame is supported；3, numerous honeybee cage；4, gauze；5, wire gauze；6, sleeve；
7, zipper door.
Detailed description of the invention
In order to describe the technology that the present invention efficiently breeds the method for vast lower limb chalcid fly using rice moth pupa as host in detail
Content, structural feature, below in conjunction with embodiment and coordinate accompanying drawing to be described further.
Embodiment 1
(1) Hosts is raised: (feed ingredient is by weight to spread feedstuff thick for one layer of 1cm in plastic casing
Calculate and be configured to by Semen Maydis powder 50 parts, 25 parts of flour, 20 parts of Testa Tritici, white sugar 3 parts, 2 parts of yeast)；
Feedstuff is uniformly spilt 1mL rice moth egg, more just covers the feedstuff of ovum grain with sieving to float in the air one layer；After 15 days
Within every 3 days, throwing a feedstuff, input amount is the larva appetite of 3 days；After larvae development to 30 day, at feedstuff
Surface spread two parallel nylon ropes, rope two ends are exposed, and the when of feeding intake thereafter, by described rope
In embedment material；After larva cocoons and pupates, described rope is together mentioned together with cocoon block；Take the rice of the upper band cocoon of rope
Chrysalis, standby；
(2) numerous honeybee cage is prepared: as it is shown in figure 1, indoor numerous honeybee, numerous honeybee cage 3 is placed in top and is provided with
Fluorescent tube 1 and bottom are provided with on the foster worm frame 2 of wire gauze 5, and described numerous honeybee cage 3 is made up of nylon wire, and
Being provided with warp layer net 4 at 2/3 height in numerous honeybee cage, 4 four angles of gauze are fixed on numerous honeybee cage by fixture
On 3, gauze 4 forms upper and lower two-layer in making numerous honeybee cage, and upper and lower two-layer communicates；
(3) the band cocoon rice moth pupa of cold preservation is obtained: the rice moth pupa of band cocoon is placed in cold preservation 1.5h at 5 DEG C, it is thus achieved that
The band cocoon rice moth pupa of cold preservation, standby；
(4) the rice moth pupa of band cocoon is obtained: on the cocoon of rice moth pupa, cut a long 2mm with shears, wide
The oval port of 1mm, makes rice moth pupa partial denudation out, it is thus achieved that the rice moth pupa of band cocoon, standby；
(5) vast lower limb chalcid fly is gathered: knit moth at Cortex cocois radicis and cause harm peak period, by the coconut palm parasitic by vast lower limb chalcid fly
Son is knitted chrysalis and is gathered go back to indoor, collects vast lower limb chalcid fly as all sources of honeybee；
(6) vast lower limb chalcid fly is raised:
A, in numerous honeybee cage on the gauze on upper strata place the rice moth pupa of band cocoon, cold preservation band cocoon rice moth pupa with
And speckle with the cotton balls of the mixed sugar liquid of concentration 10%, described mixed sugar liquid by weight 112 by sucrose
Pyroglutamate, adds distilled water diluting and makes；Rice moth pupa with cocoon and the band cocoon rice moth pupa head of cold preservation
Number ratio is 1 10；
B, putting in numerous honeybee cage by vast lower limb chalcid fly, the sex ration of the most vast lower limb chalcid fly is 13, raises close
Degree is: 8cm3/ head, controls the temperature 25 DEG C of numerous honeybee room, and relative humidity is 65%, and lighting time is 12h
(to meet the healthy development of vast lower limb chalcid fly)；Vast lower limb chalcid fly takes food mixed sugar liquid and rice moth pupa；
C, every 3 days supplementary 100 vast lower limb chalcid flies in numerous honeybee cage, replacing in every 3 days speckles with mixed sugar 1 time
The band cocoon rice moth pupa of the cotton balls of liquid, the rice moth pupa of band cocoon and cold preservation；By vast lower limb chalcid fly queen bee quantity with
The honeybee worm ratio that band cocoon rice moth pupa specific lobe is 17 of cold preservation, divides the band cocoon rice moth pupa of cold preservation and is placed in numerous honeybee
In cage on the gauze on upper strata, allow the rice moth pupa of vast lower limb chalcid fly nature parasitic strip cocoon；
D, will change after the rice moth pupa of band cocoon and the band cocoon rice moth pupa of cold preservation take out and put in conical flask,
Bottleneck seals up the nylon gauze of 100 mesh, tightens with rubber band, under the conditions of conical flask is positioned over room temperature 25 DEG C,
Ovum is to the development duration average out to 18 days of adult；
E, after the little bee eclosion of vast lower limb in insect box, be used for breeding the next generation, or anti-for field release
Control Cortex cocois radicis and knit the lepidoptera pests such as moth.
Embodiment 2
(1) Hosts is raised: (feed ingredient is by weight to spread feedstuff thick for one layer of 1.5cm in plastic casing
Part calculates and is configured to by Semen Maydis powder 50 parts, 25 parts of flour, 20 parts of Testa Tritici, white sugar 3 parts, 2 parts of yeast)；
Feedstuff is uniformly spilt 1.5mL rice moth egg, more just covers the feedstuff of ovum grain with sieving to float in the air one layer；15 days
Within the most every 3 days, throwing a feedstuff, input amount is the larva appetite of 3 days；After larvae development to 30 day, raising
Two parallel nylon ropes are spread on the surface of material, and rope two ends are exposed, and the when of feeding intake thereafter, by described
In rope embedment material；After larva cocoons and pupates, described rope is together mentioned together with cocoon block；Take the upper band cocoon of rope
Rice moth pupa, standby；
(2) numerous honeybee cage is prepared: as it is shown in figure 1, indoor numerous honeybee, numerous honeybee cage 3 is placed in top and is provided with
Fluorescent tube 1 and bottom are provided with on the foster worm frame 2 of wire gauze 5, and described numerous honeybee cage 3 is made up of nylon wire, and
Being provided with warp layer net 4 at 3/4 height in numerous honeybee cage, gauze 4 four jiaos is fixed on numerous honeybee cage 3 by fixture
On, gauze 4 forms upper and lower two-layer in making numerous honeybee cage, and upper and lower two-layer communicates；
(3) the band cocoon rice moth pupa of cold preservation is obtained: the rice moth pupa of band cocoon is placed in cold preservation 1.5h at 5 DEG C, it is thus achieved that
The band cocoon rice moth pupa of cold preservation, standby；
(4) the rice moth pupa of band cocoon is obtained: on the cocoon of rice moth pupa, cut a long 3mm with shears, wide
The oval port of 1.5mm, makes rice moth pupa partial denudation out, it is thus achieved that the rice moth pupa of band cocoon, standby；
(5) vast lower limb chalcid fly is gathered: knit moth at Cortex cocois radicis and cause harm peak period, by the coconut palm parasitic by vast lower limb chalcid fly
Son is knitted chrysalis and is gathered go back to indoor, collects vast lower limb chalcid fly as all sources of honeybee；
(6) vast lower limb chalcid fly is raised:
A, in numerous honeybee cage on the gauze on upper strata place the rice moth pupa of band cocoon, cold preservation band cocoon rice moth pupa with
And speckle with the cotton balls of the mixed sugar liquid of concentration 25%, described mixed sugar liquid by weight 112 by sucrose
Pyroglutamate, adds distilled water diluting and makes；Rice moth pupa with cocoon and the band cocoon rice moth pupa head of cold preservation
Number ratio is 1 20；
B, putting in numerous honeybee cage by vast lower limb chalcid fly, the sex ration of the most vast lower limb chalcid fly is 13, raises close
Degree is: 9cm3/ head, controls the temperature 27 DEG C of numerous honeybee room, and relative humidity is 85%, and lighting time is 15h；
Vast lower limb chalcid fly takes food mixed sugar liquid and rice moth pupa；
C, every 3 days supplementary 200 vast lower limb chalcid flies in numerous honeybee cage, replacing in every 3 days speckles with mixed sugar 1 time
The band cocoon rice moth pupa of the cotton balls of liquid, the rice moth pupa of band cocoon and cold preservation；By vast lower limb chalcid fly queen bee quantity with
The honeybee worm ratio that band cocoon rice moth pupa specific lobe is 17 of cold preservation, divides the band cocoon rice moth pupa of cold preservation and is placed in numerous honeybee
In cage on the gauze on upper strata, allow the rice moth pupa of vast lower limb chalcid fly nature parasitic strip cocoon；
D, will change after the rice moth pupa of band cocoon and the band cocoon rice moth pupa of cold preservation take out and put in conical flask,
Bottleneck seals up the nylon gauze of 200 mesh, tightens with rubber band, under the conditions of conical flask is positioned over room temperature 27 DEG C,
Ovum is to the development duration average out to 15 days of adult；
E, after the little bee eclosion of vast lower limb in insect box, be used for breeding the next generation, or anti-for field release
Control Cortex cocois radicis and knit the lepidoptera pests such as moth.
Embodiment 3
(1) Hosts is raised: (feed ingredient is by weight to spread one layer 1～1.5cm thick feedstuff in plastic casing
Amount part calculates by Semen Maydis powder 50 parts, 25 parts of flour, 20 parts of Testa Tritici, white sugar 3 parts, the preparation of 2 parts of yeast
Become)；Feedstuff is uniformly spilt 1.3mL rice moth egg, more just covers the feedstuff of ovum grain with sieving to float in the air one layer；
Within after 15 days every 3 days, throwing a feedstuff, input amount is the larva appetite of 3 days；After larvae development to 30 day,
Spread two parallel nylon ropes on the surface of feedstuff, rope two ends are exposed, and the when of feeding intake thereafter, will
In described rope embedment material；After larva cocoons and pupates, described rope is together mentioned together with cocoon block；Take the upper band of rope
The rice moth pupa of cocoon, standby；
(2) numerous honeybee cage is prepared: as it is shown in figure 1, indoor numerous honeybee, numerous honeybee cage 3 is placed in top and is provided with
Fluorescent tube 1 and bottom are provided with on the foster worm frame 2 of wire gauze 5, and described numerous honeybee cage 3 is made up of nylon wire, and
Being provided with warp layer net 4 at 3/4 height in numerous honeybee cage, 4 four angles of gauze are fixed on numerous honeybee cage by fixture
On 3, gauze 4 forms upper and lower two-layer in making numerous honeybee cage, and upper and lower two-layer communicates；
(3) the band cocoon rice moth pupa of cold preservation is obtained: the rice moth pupa of band cocoon is placed in cold preservation 1.5h at 5 DEG C, it is thus achieved that
The band cocoon rice moth pupa of cold preservation, standby；
(4) the rice moth pupa of band cocoon is obtained: on the cocoon of rice moth pupa, cut a long 2.5mm with shears,
The oval port of wide 1.3mm, makes rice moth pupa partial denudation out, it is thus achieved that the rice moth pupa of band cocoon, standby；
(5) vast lower limb chalcid fly is gathered: knit moth at Cortex cocois radicis and cause harm peak period, by the coconut palm parasitic by vast lower limb chalcid fly
Son is knitted chrysalis and is gathered go back to indoor, collects vast lower limb chalcid fly as all sources of honeybee；
(6) vast lower limb chalcid fly is raised:
A, in numerous honeybee cage on the gauze on upper strata place the rice moth pupa of band cocoon, cold preservation band cocoon rice moth pupa with
And speckle with the cotton balls of the mixed sugar liquid of concentration 20%, described mixed sugar liquid by weight 112 by sucrose
Pyroglutamate, adds distilled water diluting and makes；Rice moth pupa with cocoon and the band cocoon rice moth pupa head of cold preservation
Number ratio is 1 15；
B, putting in numerous honeybee cage by vast lower limb chalcid fly, the sex ration of the most vast lower limb chalcid fly is 13, raises close
Degree is: 9cm3/ head, controls the temperature 26 DEG C of numerous honeybee room, and relative humidity is 75%, and lighting time is 14h；
Vast lower limb chalcid fly takes food mixed sugar liquid and rice moth pupa；
C, every 3 days supplementary 150 vast lower limb chalcid flies in numerous honeybee cage, replacing in every 3 days speckles with mixed sugar 1 time
The band cocoon rice moth pupa of the cotton balls of liquid, the rice moth pupa of band cocoon and cold preservation；By vast lower limb chalcid fly queen bee quantity with
The honeybee worm ratio that band cocoon rice moth pupa specific lobe is 17 of cold preservation, divides the band cocoon rice moth pupa of cold preservation and is placed in numerous honeybee
In cage on the gauze on upper strata, allow the rice moth pupa of vast lower limb chalcid fly nature parasitic strip cocoon；
D, will change after the rice moth pupa of band cocoon and the band cocoon rice moth pupa of cold preservation take out and put in conical flask,
Bottleneck seals up the nylon gauze of 150 mesh, tightens with rubber band, under the conditions of conical flask is positioned over room temperature 26 DEG C,
Ovum is to the development duration average out to 17 days of adult；
E, after the little bee eclosion of vast lower limb in insect box, be used for breeding the next generation, or anti-for field release
Control Cortex cocois radicis and knit the lepidoptera pests such as moth.
Choose the rice moth cocoon containing rice moth pupa as host, it is judged that the larva inside rice moth cocoon has pupated
Standard be that the rice moth larva explanation in the cocoon that hardness is bigger has been pupated, and the rice inside softer rice moth cocoon
Moth larvae is not also pupated.
As it is shown in figure 1, described numerous honeybee cage is provided with sleeve 6, sleeve barrel dliameter is 12～14cm, a length of 30～
40cm, is arranged on the top before cage.Sleeve is the little opening of cage, supplement or change mixed sugar liquid,
The escape of vast lower limb chalcid fly it is possible to prevent when putting into or take out rice moth pupa.
It is additionally provided with zipper door 7 on described numerous honeybee cage, high the 20 of zipper door～25cm, bottom wide 15～20cm.
Before numerous honeybee cage, open a semi-circular door from the bottom up, for the big opening of zip mode of cage, be provided with two
Slider of zipper fastener, is sewed with described sleeve in semi-circular door.Zipper door is easy to put into parasitic wasp, host in cage,
Taking out host, in cleaning cage, the cleaning etc. of dead parasitic wasp and cage operates.
The rice moth pupa of described band cocoon is to cut one long 2～3mm, wide 1～1.5 on rice moth cocoon with shears
The oval port of mm, makes rice moth pupa partial denudation out, mainly supplies vast lower limb chalcid fly and takes food host's body fluid
Supplement the nutrients.
The rice moth pupa of band cocoon supplying at 5 DEG C vast lower limb chalcid fly parasitic after cold preservation 1.5h, K cryogenic treatment makes to be in
Rice moth pupa mobility in cocoon reduces, and parasitic wasp can be to host's rice moth pupa in the case of consuming minimum muscle power
Benumb successfully, and then host could be carried out parasitism, make vast lower limb chalcid fly that the parasitic rate of rice moth pupa to be carried significantly
High.
Process of the test:
1, the vast lower limb chalcid fly Choice tests to different days rice moth pupa
The little bee imago of vast lower limb 40 (sex ration is 1 3) of 7 ages in days is introduced numerous honeybee cage, will be placed with
The culture dish of 15 1,2,3,4,5,6,7,8,9,10,11 age in days rice moth pupas is radially placed
On the gauze on numerous honeybee cage upper strata, to speckle with the cotton balls extra-nutrition of 20% mixed sugar liquid, parasitic 24h, respectively
Process is repeated 3 times.The vast lower limb chalcid fly parasitic rate to different days rice moth pupa is added up after sprouting wings to parasitic wasp.
The table 1 vast lower limb chalcid fly parasitic rate to different days rice moth pupa
Concrete outcome is as shown in table 1, and from table 1, vast lower limb chalcid fly can the rice moth of parasitic 1～10 ages in days
Pupa, can not the rice moth pupa (parasitic rate is 0%) of parasitic 11 ages in days.Therefore, in order to avoid the age in days of rice moth pupa
More than 10 ages in days, cocooing simultaneously because rice moth mature larva can be weaved silk, the larvae pupation time is irregular, should be every
The rice moth pupa choosing band cocoon for 10 days from the rice moth culture medium being in the stage of pupating is parasitic for vast lower limb chalcid fly.
2, the parasitic rate of different disposal rice moth pupa is tested by vast lower limb chalcid fly
The little bee imago of vast lower limb 40 (sex ration is 1 3) of 7 ages in days is introduced in vial, puts into
The rice moth pupa of 30 different disposal: the pupa of band cocoon, the pupa of band cocoon (cut one with shears on rice moth cocoon
Individual long 3mm, the oval port of wide 1mm, makes rice moth pupa partial denudation out), at 5 DEG C of cold preservation 0.5h
Band cocoon pupa, the band cocoon pupa of 5 DEG C of cold preservation 1h, the band cocoon pupa of 5 DEG C of cold preservation 1.5h, the band cocoon of 5 DEG C of cold preservation 2h
Pupa, to speckle with the cotton balls extra-nutrition of 20% mixed sugar liquid, parasitic 24h, each process is repeated 3 times.To posting
The vast lower limb chalcid fly parasitic rate to different disposal rice moth pupa is added up after raw bee eclosion.
The table 2 vast lower limb chalcid fly parasitic rate to different disposal rice moth pupa
Concrete outcome is as shown in table 2, and from table 2, vast lower limb chalcid fly is to the band cocoon at 5 DEG C of cold preservation 1.5h
The parasitic rate of pupa is the highest, is 90.01%, is significantly higher than the band cocoon pupa at 5 DEG C of cold preservations 1h, 2h, 0.5h
With the parasitic rate (76.35%, 74.00%, 72.11%, 71.16%) of the pupa of band cocoon, the most extensively
The parasitic rate without the band cocoon rice moth pupa of cold preservation of thigh chalcid fly pair is relatively low is 43.47%, and by the rice of band cocoon
Chrysalis is through significantly improving this parasitic wasp parasitic rate (90.01%) to rice moth pupa at 5 DEG C of cold preservation 1.5h.
The parasitic rate of this parasitic wasp band cocoon rice moth pupa to cutting mouth through shears is 71.16%.Rice moth by band cocoon
Pupa directly feeds vast lower limb chalcid fly at 5 DEG C after cold preservation 1.5h parasitic, and its parasitic rate is significantly higher than cuts mouth to band
The parasitic rate of the pupa of cocoon, eliminate time-consuming the rice moth pupa of band cocoon is cut mouth after to supply this parasitic wasp parasitic
Tedious steps.Meanwhile, the present invention use the rice moth pupa of band cocoon, cold preservation band cocoon rice moth pupa by most preferably
Specific lobe is 1 10～20 to be applied in combination, and the rice moth pupa of band cocoon is mainly as the vast lower limb of supplying nutrient source
Chalcid fly, 5 DEG C of cold preservation 1.5h band cocoon rice moth pupas supply vast lower limb chalcid fly parasitism, two kinds of differences mainly as host
Rice moth pupa after process is applied in combination, it is achieved thereby that the biological control of vast lower limb chalcid fly.
3, the different stocking density impacts on vast lower limb chalcid fly reproductive capacity
Indoor numerous honeybee, numerous honeybee cage is placed on the foster worm frame being provided with fluorescent tube and wire gauze, puts in numerous honeybee cage
Enter vast lower limb chalcid fly 800,1000,1200 (sex ration is 1 3), within every 3 days, in cage, supplement 100
Vast lower limb chalcid fly；Upper strata in cage is put into and is speckled with 20% mixed sugar liquid (sucrose pyroglutamate=1 1
2, add the mixed liquor of distilled water diluting) the culture dish of cotton balls and the rice moth pupa of 100 band cocoons allow this
Parasitic wasp takes food mixed sugar liquid and rice moth pupa to supplement the nutrients.Within every 3 days, in numerous honeybee cage, supplement 100 vast lower limbs
Chalcid fly, replacing in every 3 days speckles with the cotton balls of mixed sugar liquid, the rice moth pupa of band cocoon and the band cocoon of cold preservation for 1 time
Rice moth pupa；By the honeybee worm ratio that band cocoon rice moth pupa specific lobe is 17 of vast lower limb chalcid fly queen bee quantity with cold preservation,
The band cocoon rice moth pupa of cold preservation is divided and is placed in numerous honeybee cage on the culture dish of upper strata gauze, make vast lower limb chalcid fly natural
The rice moth pupa of parasitic strip cocoon；After 3 days, the rice moth pupa of this batch of rice moth pupa and band cocoon is taken out and put into conical flask
In, nylon gauze sealed up by bottleneck rubber band；The rice moth pupa more changing to the new band cocoon at 5 DEG C of cold preservation 1.5h supplies
This parasitic wasp is parasitic, and the rice moth pupa changing to new band cocoon takes food for parasitic wasp, circulates abovementioned steps.Treat
Parasitic wasp in insect box sprouts wings and adds up bee yield.
Result of the test shows 800 vast lower limb chalcid flies of each numerous honeybee cage, and the bee yield of one month is 12900
Head；1000 vast lower limb chalcid flies of each numerous honeybee cage, the bee yield of one month is 16800；Each numerous honeybee
The vast lower limb chalcid fly of 1200, cage, the bee yield of one month is 16650.The above results shows, Mei Gefan
It (is 9000cm according to the volume of numerous honeybee cage that honeybee cage should raise 1000 vast lower limb chalcid flies3, draw optimal raising
Foster density is 9cm3/ head), stocking density is low is unfavorable for making full use of of numerous honeybee cage, and stocking density is the highest also can
Reduce the filial generation quantity of every queen bee.
4, the breeding effect difference of vast lower limb chalcid fly raised by monolayer numerous honeybee cage and bilayer numerous honeybee cage
Indoor numerous honeybee, numerous for monolayer honeybee cage (numerous honeybee cage is not provided to upper and lower two-layer) is placed in top and is provided with
Fluorescent tube and bottom are provided with on the foster worm frame of wire gauze, put into vast lower limb chalcid fly 1000 (wide in numerous honeybee cage
The sex ration of thigh chalcid fly is about 1 3), within every 3 days, in cage, supplement 100 vast lower limb chalcid flies；In cage
Bottom is put into and is speckled with 20% mixed sugar liquid (sucrose pyroglutamate=1 12, add distilled water diluting
Mixed liquor) the culture dish of cotton balls and the rice moth pupa of 100 band cocoons (cut on rice moth cocoon with shears
One is about 3mm, the oval port of wide about 1mm, makes rice moth pupa partial denudation out), allow this parasitic wasp
Take food mixed sugar liquid and rice moth pupa to supplement the nutrients；Supplementing mixed sugar liquid every day, replacing in every 3 days speckles with for 1 time
The cotton balls of mixed sugar liquid and the rice moth pupa of band cocoon.Every 3 days by vast lower limb chalcid fly queen bee quantity and band cocoon
Rice moth pupa is divided than by the rice moth pupa of the band cocoon at 5 DEG C of cold preservation 1.5h than the honeybee worm being 17 and is placed in culture dish
In, it is placed in cage on the gauze of bottom, allows the rice moth pupa of vast lower limb chalcid fly nature parasitic strip cocoon, should after 3 days
In batch rice moth pupa and cage, the rice moth pupa of band cocoon takes out and puts in conical flask, and nylon sealed up by bottleneck rubber band
Gauze, the rice moth pupa more changing to the new band cocoon at 5 DEG C of cold preservation 1.5h is parasitic for parasitic wasp, circulates aforementioned step
Suddenly.
Result of the test shows 1000 vast lower limb chalcid flies of each monolayer numerous honeybee cage, and the bee yield of one month is
13145.Test 3 (the different stocking density impacts on vast lower limb chalcid fly reproductive capacity) from the present invention and understand every
1000 vast lower limb chalcid flies of individual bilayer numerous honeybee cage, the bee yield of one month is 16800.Therefore, arrange
Double-deck numerous honeybee cage can improve the bee yield of vast lower limb chalcid fly.
5, supplement the nutrients the impact on vast lower limb chalcid fly Adult worms producting eggs amount
Being introduced in glass tubing by the vast lower limb chalcid fly 4 (sex ration is 1 3) just sprouted wings, every day, supply was stained with
Have 20% mixed sugar liquid (sucrose pyroglutamate=1 12 add the mixed liquor of distilled water diluting), 20%
Sucrose, 20% glucose, 20% fructose, the cotton mass of 20% Mel, change to new rice moth pupa 25, will
The rice moth pupa changed is dissected under stereomicroscope, adds up egg laying amount, until adult is dead, each process
It is repeated 10 times.
The different impact supplemented the nutrients on vast lower limb chalcid fly Adult worms producting eggs amount of table 3
From table 3, the egg laying amount of vast lower limb chalcid fly is had a significant impact by different exogenous nutrition.The present invention
The egg laying amount of mixed sugar liquid is the highest, is 287 ± 20, is significantly higher than at glucose, sucrose, fructose and Mel
The egg laying amount of reason group.Therefore, (sucrose pyroglutamate=1 12, add steaming should to select mixed sugar liquid
Evaporate water-reducible mixed liquor) raise this parasitic wasp.
6, rice moth pupa and Cortex cocois radicis are knitted the vast lower limb chalcid fly that chrysalis breeds and Cortex cocois radicis is knitted the parasitic ability of chrysalis are compared
Introduce 7 ages in days in vial knits, with rice moth pupa or Cortex cocois radicis, the vast lower limb chalcid fly 40 that chrysalis breeds
(male and female=1 3), put into 50 Cortex cocois radiciss and knit chrysalis, to speckle with the cotton balls Replacement Battalion of 20% mixed sugar liquid
Support, parasitic 24h, be repeated 3 times, after parasitic wasp sprouts wings, add up their parasitic rate.
The parasitic rate of chrysalis knitted by Cortex cocois radicis by the vast lower limb chalcid fly of table 4 different host breeding
With the vast lower limb chalcid fly of rice moth pupa breeding, Cortex cocois radicis being knitted the parasitic rate of chrysalis as known from Table 4 is
94.00% ± 2.00%, knit the vast lower limb chalcid fly of chrysalis breeding with Cortex cocois radicis Cortex cocois radicis knitted the parasitic rate of chrysalis and be
92.67% ± 2.31%, with the vast lower limb chalcid fly of rice moth pupa breeding and knit, with Cortex cocois radicis, the vast lower limb chalcid fly that chrysalis breeds
The parasitic rate difference that Cortex cocois radicis is knitted chrysalis is not notable, the vast lower limb chalcid fly utilizing rice moth pupa to breed is described and with coconut palm
It is suitable, in the vast lower limb chalcid fly control of breeding that son knits the vast lower limb chalcid fly of chrysalis breeding effect when moth knitted by parasitic Cortex cocois radicis
When causing harm of moth knitted by Cortex cocois radicis processed, can select rice moth pupa host as an alternative.
Vast lower limb chalcid fly is the natural enemy of the highly advantageous value of class.Present invention firstly provides with at 5 DEG C of cold preservation 1.5h
The rice moth pupa host as an alternative of band cocoon raise vast lower limb chalcid fly, can this parasitic wasp of large-scale breeding.Realize
The continuous raising of this parasitic wasp, on the one hand meets experimental study needs, and on the other hand, the breeding of scale obtains
Parasitic wasp can apply to prevent and treat Cortex cocois radicis knit moth, rice leaf roller, diamondback moth, mythimna separata, Prodenia litura,
Many Species of Lepidopterous Insect Pests such as rice green caterpillar, rice plant skipper, it is achieved to biological control of pests.
Above disclosed only presently preferred embodiments of the present invention, can not limit the present invention with this certainly
Interest field, the equivalent variations therefore made according to the claims in the present invention, still fall within what the present invention was contained
Scope.
Claims (3)
1. the method efficiently breeding vast lower limb chalcid fly using rice moth pupa as host, it is characterised in that include following step
Rapid:
(1) Hosts is raised: spread one layer 1～1.5cm thick feedstuff in plastic casing；On feedstuff uniformly
Spill 1～1.5mL rice moth egg, more just cover the feedstuff of ovum grain with sieving to float in the air one layer；After 15 days every 3 days
Throwing a feedstuff, input amount is the larva appetite of 3 days；After larvae development to 30 day, on the surface of feedstuff
Spreading two parallel nylon ropes, rope two ends are exposed, and the when of feeding intake thereafter, by described rope embedment material
In；After larva cocoons and pupates, described rope is together mentioned together with cocoon block；Take the rice moth pupa of the upper band cocoon of rope,
Standby；
(2) prepare numerous honeybee cage: indoor numerous honeybee, numerous honeybee cage is placed in top and is provided with fluorescent tube and bottom setting
Having on the foster worm frame of wire gauze, described numerous honeybee cage is made up of nylon wire, and 2/3～3/4 height in numerous honeybee cage
Place is provided with warp layer net, and gauze is fixed in numerous honeybee cage by fixture, and gauze is formed up and down in making numerous honeybee cage
Two-layer, upper and lower two-layer communicates；
(3) the band cocoon rice moth pupa of cold preservation is obtained: the rice moth pupa of band cocoon is placed in cold preservation 1.5h at 5 DEG C, it is thus achieved that
The band cocoon rice moth pupa of cold preservation, standby；
(4) the rice moth pupa of band cocoon is obtained: on the cocoon of rice moth pupa, cut one long 2～3mm with shears,
Wide 1～the oval port of 1.5mm, make rice moth pupa partial denudation out, it is thus achieved that the rice moth pupa of band cocoon,
Standby；
(5) vast lower limb chalcid fly is gathered: knit moth at Cortex cocois radicis and cause harm peak period, by the coconut palm parasitic by vast lower limb chalcid fly
Son is knitted chrysalis and is gathered go back to indoor, collects vast lower limb chalcid fly as all sources of honeybee；
(6) vast lower limb chalcid fly is raised:
A, in numerous honeybee cage on the gauze on upper strata place the rice moth pupa of band cocoon, the band cocoon rice moth pupa of cold preservation
And speckling with the cotton balls of the mixed sugar liquid of concentration 10%～25%, described mixed sugar liquid is by weight 112
By sucrose pyroglutamate, add distilled water diluting and make；Rice moth pupa with cocoon and the band cocoon of cold preservation
Rice moth pupa specific lobe is 1 10～20；
B, putting in numerous honeybee cage by vast lower limb chalcid fly, the sex ration of the most vast lower limb chalcid fly is 13, raises
Density is: 8～10cm3/ head, controls the temperature 25 DEG C～27 DEG C of numerous honeybee room, and relative humidity is 65%～85%,
Lighting time is 12～15 hours；Vast lower limb chalcid fly takes food mixed sugar liquid and rice moth pupa；
C, every 3 days supplementary 100～200 vast lower limb chalcid flies in numerous honeybee cage, replacing in every 3 days speckles with for 1 time
The band cocoon rice moth pupa of the cotton balls of mixed sugar liquid, the rice moth pupa of band cocoon and cold preservation；By vast lower limb chalcid fly queen bee
Quantity and the honeybee worm ratio that band cocoon rice moth pupa specific lobe is 17 of cold preservation, divide the band cocoon rice moth pupa of cold preservation and put
In numerous honeybee cage in the culture dish of upper strata gauze, allow the rice moth pupa of vast lower limb chalcid fly nature parasitic strip cocoon；
D, will change after the rice moth pupa of band cocoon and the band cocoon rice moth pupa of cold preservation take out and put in conical flask,
Bottleneck seals up the nylon gauze of 100～200 mesh, tightens with rubber band, conical flask is positioned over room temperature 25 DEG C～
Under the conditions of 27 DEG C, the development duration of ovum to adult is 15～18 days；
E, after the little bee eclosion of vast lower limb in conical flask, take off the nylon gauze of bottleneck, put in dependent insect cage,
Being placed with the cotton balls speckling with mixed sugar liquid in dependent insect cage, dependent insect cage is provided above fluorescent tube, allows vast lower limb chalcid fly climb
Go out conical flask, concentrate on for breeding the next generation in dependent insect cage, or for field release preventing and treating lepidoptera pest.
2. the method efficiently breeding vast lower limb chalcid fly using rice moth pupa as host as claimed in claim 1, its
It is characterised by: the feed ingredient in described step (1) calculates by weight by Semen Maydis powder 50 parts, flour 25
Part, 20 parts of Testa Tritici, white sugar 3 parts, 2 parts of yeast are configured to.
3. the method efficiently breeding vast lower limb chalcid fly using rice moth pupa as host as claimed in claim 1, its
It is characterised by: it is 9cm that described step (6) raises the stocking density of vast lower limb chalcid fly3/ head.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610402099.7A CN105875519B (en) | 2016-06-08 | 2016-06-08 | Method for efficiently reproducing brachymeria lasus by taking corcyra cephalonica pupae as hosts |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610402099.7A CN105875519B (en) | 2016-06-08 | 2016-06-08 | Method for efficiently reproducing brachymeria lasus by taking corcyra cephalonica pupae as hosts |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105875519A true CN105875519A (en) | 2016-08-24 |
| CN105875519B CN105875519B (en) | 2017-02-08 |
Family
ID=56711602
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610402099.7A Active CN105875519B (en) | 2016-06-08 | 2016-06-08 | Method for efficiently reproducing brachymeria lasus by taking corcyra cephalonica pupae as hosts |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105875519B (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106386712A (en) * | 2016-11-23 | 2017-02-15 | 贵州省烟草公司遵义市公司绥阳县分公司 | Feeding method and device thereof for feeding arma chinensis by prey insects |
| CN107466975A (en) * | 2017-09-25 | 2017-12-15 | 广西壮族自治区农业科学院甘蔗研究所 | A kind of multi-functional rice moth breeding apparatus |
| CN108522435A (en) * | 2018-06-08 | 2018-09-14 | 河南农业大学 | A kind of device and parasitic ability evaluation method of detection the parasitism performance of parasitic wasp |
| CN108935335A (en) * | 2018-06-25 | 2018-12-07 | 中国热带农业科学院环境与植物保护研究所 | A method of moth is knitted using spark thigh chalcid fly prevention and treatment coconut |
| CN109006712A (en) * | 2018-09-12 | 2018-12-18 | 安徽师范大学 | A kind of method for breeding and breeding apparatus of Ling Mushi drosophila |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH05103563A (en) * | 1991-10-11 | 1993-04-27 | Sumitomo Chem Co Ltd | Method for rearing parasitic bee |
| CN105145487A (en) * | 2014-07-23 | 2015-12-16 | 中国科学院动物研究所 | Trichogramma japonicun ashmead artifical breeding production method |
| CN105165734A (en) * | 2015-09-28 | 2015-12-23 | 中国热带农业科学院环境与植物保护研究所 | Method for mass reproduction of Bracon adoxophyesi Mimanikawa |
-
2016
- 2016-06-08 CN CN201610402099.7A patent/CN105875519B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH05103563A (en) * | 1991-10-11 | 1993-04-27 | Sumitomo Chem Co Ltd | Method for rearing parasitic bee |
| CN105145487A (en) * | 2014-07-23 | 2015-12-16 | 中国科学院动物研究所 | Trichogramma japonicun ashmead artifical breeding production method |
| CN105165734A (en) * | 2015-09-28 | 2015-12-23 | 中国热带农业科学院环境与植物保护研究所 | Method for mass reproduction of Bracon adoxophyesi Mimanikawa |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106386712A (en) * | 2016-11-23 | 2017-02-15 | 贵州省烟草公司遵义市公司绥阳县分公司 | Feeding method and device thereof for feeding arma chinensis by prey insects |
| CN107466975A (en) * | 2017-09-25 | 2017-12-15 | 广西壮族自治区农业科学院甘蔗研究所 | A kind of multi-functional rice moth breeding apparatus |
| CN108522435A (en) * | 2018-06-08 | 2018-09-14 | 河南农业大学 | A kind of device and parasitic ability evaluation method of detection the parasitism performance of parasitic wasp |
| CN108935335A (en) * | 2018-06-25 | 2018-12-07 | 中国热带农业科学院环境与植物保护研究所 | A method of moth is knitted using spark thigh chalcid fly prevention and treatment coconut |
| CN109006712A (en) * | 2018-09-12 | 2018-12-18 | 安徽师范大学 | A kind of method for breeding and breeding apparatus of Ling Mushi drosophila |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105875519B (en) | 2017-02-08 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105875519A (en) | Method for efficiently reproducing brachymeria lasus by taking corcyra cephalonica pupae as hosts | |
| CN105165734B (en) | A kind of method of Adoxophyes spp cocoon honeybee biological control | |
| CN101796940A (en) | Super flour weevil culture technology | |
| CN103155906A (en) | Aphidoletes aphidimyza mass reproduction technology and application technology | |
| CN112868606B (en) | Method for indoor raising sugarcane striped rice borers or dibbler | |
| CN105746435B (en) | A kind of method using rice moth egg breeding chrysopa septempunctata larva | |
| CN107581160A (en) | A kind of artificial scale breeding method of mythimna separata | |
| CN103843726B (en) | A kind of artificial propagation fly pupa hires the method for chalcid fly | |
| CN101810154A (en) | Method for industrially producing zophobas morio | |
| KR20160042216A (en) | Method for Stimulating Rapid Growth of Tenebrio molitor Larvae | |
| CN104996364A (en) | Artificial culture method of fly larvae | |
| CN109769755A (en) | A kind of simple and fast propagation method of spotted wing drosophila pupa parasitic wasp hair angle clavicorn serphid | |
| CN113317091A (en) | Method for breeding Spodoptera frugiperda advantage natural enemy gloeosporioides | |
| CN113925023A (en) | Method for breeding black soldier fly commercial larvae by mixing chicken manure and lysimachia foenum-graecum as feed | |
| CN106804531B (en) | Comprehensive breeding method for cuora flavomarginata from parent cuora to adult cuora flavomarginata | |
| CN111328773A (en) | Method for breeding Spodoptera frugiperda larvae | |
| CN106889021A (en) | A kind of cultural method of frog | |
| CN107493972A (en) | A kind of pure artificial cultivation method of termite series collybia albuminosa | |
| CN102124999B (en) | Method for raising experimental population of Rhynchophorus ferrugineus | |
| CN102771446B (en) | Propagation method of Diglyphus isaea by use of Chromatomyia horticola | |
| CN114431194B (en) | Artificial breeding method of orius viridis | |
| CN111066732B (en) | Method for breeding orius sauteri | |
| CN108740611B (en) | Artificial feed for dichocrocis punctiferalis larvae, preparation method of artificial feed and dichocrocis punctiferalis breeding method | |
| CN107853253B (en) | Indoor circulating breeding device and method for macrobrachium giganteum | |
| CN111642465A (en) | Breeding method of Altai hepialus larvae |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |