CN102124999B - Method for raising experimental population of Rhynchophorus ferrugineus - Google Patents
Method for raising experimental population of Rhynchophorus ferrugineus Download PDFInfo
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- CN102124999B CN102124999B CN2011100347687A CN201110034768A CN102124999B CN 102124999 B CN102124999 B CN 102124999B CN 2011100347687 A CN2011100347687 A CN 2011100347687A CN 201110034768 A CN201110034768 A CN 201110034768A CN 102124999 B CN102124999 B CN 102124999B
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Abstract
The invention relates to a method for raising an experimental population of Rhynchophorus ferrugineus. The method comprises the following steps of: performing eclosion on pupae of Rhynchophorus ferrugineus to be raised into worms, then putting each pair of adult worms which are taken as a group into a container with hydromel for mating and oviposition, routinely disinfecting eggs and then hatching to obtain larvae, inoculating the larvae into a Rhynchophorus ferrugineus larvae raising container loaded with a semi-artificial feed of the Rhynchophorus ferrugineus larvae, and putting into a healthy worm raising room for raising until the larvae are pupated. The method is simple, the life history of the Rhynchophorus ferrugineus is finished indoors by adopting the semi-artificial feed, and a large quantity of intact eggs, accurate larvae at various ages and pupae are obtained under the condition of not hurting the eggs and the larvae; and the method has the characteristics of short development duration, high survival rate and the like, effectively and greatly shortens the generation cycle, and provides adequate experiment populations for completing the deep and systemic research on the Rhynchophorus ferrugineus in the fields such as biology, ecology and control technologies and the like.
Description
Technical field
The invention belongs to biological technical field, be specifically related to a kind of raising method of red plam weevil experimental population.
Background technology
Red plam weevil Rhynchophorus ferrugineus (Olivier) belongs to coleoptera; Culculionidae; Another name rust palm resembles, the latent beak of coconut resembles, coconut beetle, Asia palm weevil etc.; Be a kind of worldwide major pest of babassu of mainly causing harm, in the Asia, all there is distribution in Europe, Africa, domestic Hainan, Guangxi, Guangdong, Zhejiang, Taiwan, Yunnan, Tibet 12 provinces, cities and autonomous regions such as (Motuos) of being distributed in.
Red plam weevil was bred 2-3 generation in 1 year in China, generation overlap, and all can endanger the whole year; 3-15 is given birth to the babassu maximum that causes harm; Mainly bore moth with larva and cause harm, adult lays eggs at the young tender part of plant or wound, slight crack place, and ovum hatching back larva is bored moth and feeding plant tissue.The initial stage non-evident sympton, trunk is usually eaten hollow when waiting to find.
In recent years, babassu is because of its tree-like grace and have Tropic Affair, and cultivated area constantly enlarges, and single continuous planting patterns is that red plam weevil provides sufficient food source.Because of the red plam weevil larva is a borer pest, the chemical control weak effect, and use chemical pesticide not only to cause insect to develop immunity to drugs, and ecotope is caused severe contamination, also can constitute a threat to other biological.
Once there were forefathers that the raising method of red plam weevil is studied, the flesh tissue of sugarcane or palm plant was put into culture dish in order to raise red plam weevil adult and larva.This raising method can make red plam weevil accomplish from generation to generation, but has problems.At first, sugarcane production, maturation have certain seasonality, can not satisfy the demands, and palm plant costs an arm and a leg, be used to raise the red plam weevil cost too high, the waste resource; Secondly, adult produces ovum at sugarcane or palm plant tissue, and ovum comes to harm when picking easily, reduces the incubation rate of ovum; The 3rd, inspection larvae development and when survival need be cut sugarcane open or palm plant tissue is searched, and it is little to go out to incubate the young tender body of larva, and very approaching with the color of plant tissue, not only wastes time and energy during searching, and injures larva easily.It is 21.67% (Wang Feng, Ju Ruiting, Li Yuezhong that the use sugarcane of putting down in writing in the existing bibliographical information is raised the survival rate of the whole generation of red plam weevil; Xu Ying, Chi Xingzhen opens brightness. and utilize sugarcane to raise the technology of red plam weevil. insect knowledge; 2009,46 (6): 967-969.); In addition, owing to be difficult for observing directly larva, and then can not obtain each instar larvae accurately.Moreover various with sugarcane or palm plant tissue raising red plam weevil material, operation is numerous and diverse, needs bigger space during mass rearing, suitable laboratory mass rearing.
Summary of the invention
The raising method that the objective of the invention is to be directed against the deficiency of prior art and a kind of red plam weevil experimental population is provided; The employing meridic diet is in the history of life of indoor completion red plam weevil; Under the situation that does not injure ovum and larva, can obtain a large amount of intact ovum, each instar larvae and pupa accurately; Have characteristics such as development duration weak point, survival rate height; Effectively the generation cycle is shortened greatly, for the system research of accomplishing the fields such as biology, ecology and Prevention Technique of red plam weevil of going deep into provides sufficient experimental population.
The technical scheme that the present invention adopted:
A kind of raising method of red plam weevil experimental population, its concrete steps are following:
(1) red plam weevil pupa that will be to be raised is 25～28 ℃ in temperature, and adult sprouts wings under the condition of humidity 65～85%; Adult after will sprouting wings is then put into the container that has 20% hydromel for one group respectively by every pair of adult and carries out mate and oviposit; With hatching larva behind the ovum routine disinfection.
In the adult mate and oviposit period, adding little group, to dip in full mass fraction be the adsorbate of the 20% hydromel adult of feeding, and hydromel can prolong the life-span of adult, and described adsorbate is cotton or gauze.
Because the red plam weevil larva has the habit of autotomy, therefore will go out to incubate larva at set intervals and separate with ovum, so promptly guaranteed the amount of laying eggs; Again can be so that the brooding time basically identical of ovum; Help obtaining more neat insect in the length of time, general interval time is short more, and its length of time is neat more; But for easy to operate, general every separated 12-24h will go out to incubate larva and ovum separates once.
(2) the larva access of hatching is equipped with in the red plam weevil larva raising container of red plam weevil larva meridic diet; Putting into healthy supporting between worm raises; The allotment meridic diet is 5～8 times in whole larva raising process, to guarantee the sufficient and fresh of forage volume, raises until larvae pupation.Said meridic diet is to be advanced stirring and evenly mixing and processed by coconut leaf powder, bagasse, oat, corn flour, legumin, glucose, sucrose, agar, yeast, Webster salt, cholesterol, ascorbic acid, potassium sorbate and water, and wherein each component is by weight: coconut leaf powder 50, bagasse 30, oat 20, corn flour 10, legumin 15, glucose 10, sucrose 10, agar 10, yeast 5, Webster salt 2, cholesterol 1, ascorbic acid 1, potassium sorbate 1, water 400.
The cleanliness factor that said health is supported between worm is thousand grades, and temperature control is at 27～29 ℃, and control is wet 65～85%.
When allocating meridic diet the last time, in feed, add coir, auxiliary mature larva is done cocoon.
Coconut leaf powder in the raw material is chosen the open-air coconut leaf that comes off naturally or be about to come off and after oven dry is pulverized, is made.Elder generation's stirring, mixing before each larva meridic diet drops into; Divide again and install in the red plam weevil larva raising container; Compacting; In the middle of feed, bore a duct with aseptic thin glass bar, the red plam weevil larva is placed in the middle duct of feed carefully, place healthy supporting between worm to raise under the no optical condition in red plam weevil larva raising container then.
Technology of the present invention is simple; Adopt the raw material wide material sources, do not receive the history of life of the meridic diet of season limit at indoor completion red plam weevil; Under the situation that does not injure ovum and larva, can obtain a large amount of intact ovum, each instar larvae and pupa accurately; Have characteristics such as development duration weak point, survival rate height, effectively the generation cycle is shortened greatly, for the system research of accomplishing the fields such as biology, ecology and Prevention Technique of red plam weevil of going deep into provides sufficient experimental population.
Embodiment
The method of the existing artificial single feed for nursing of the present invention is compared, and has all done elaborately plannedly at living environment, feed and the selection of the red plam weevil liquid that has additional nutrients, and through result of the test, has also verified the effect of its breakthrough.
The DIFFERENT FEED prescription is raised the effect of red plam weevil larva and is seen table 1.
Table 1
The present invention is used for the raising of red plam weevil experimental population, can guarantee effectively that survival rate of larvae is more than 60% under this raising method and environment; Larva is gone through the phase and is lower than 65 days; Pupation rate is not less than 95%; The time ratio of a generation of completion is raised at most with sugarcane and is shortened more than 50 day (approximately needing about 150 days with generation of sugarcane raising completion usually), and relative cost reduces; Because these raw material do not receive the restriction in season, the preparation of suiting measures to local conditions at any time seasonal shortage can not occur and has influence on the artificial feeding of red plam weevil larva, thereby indoor continuous mass rearing red plam weevil larva is fully ensured.
Next on the living environment of red plam weevil, through test of many times, sets up healthy supporting between worm for the nursing of larva, and cleanliness factor is thousand grades between the foster worm of this health, has functions such as temperature control, control light application time and air circulation.
It is right that the present invention mixes into the adult male and female of sprouting wings, and puts into container and feed with 20% hydromel, and only put 1 pair of adult in every container.So promptly guaranteed the supply of nutrition, avoided many again, can accomplish mate and oviposit thus a mutual interference.
In the present invention; Elder generation's stirring, mixing before each larva meridic diet drops into divide to install in the red plam weevil larva raising container compacting again; In the middle of feed, bore a duct with aseptic thin glass bar; The red plam weevil larva is placed in the middle duct of feed carefully, places healthy supporting between worm to raise under the no optical condition in red plam weevil larva raising container then, help feed of red plam weevil larva and assimilating of various nutrition like this.
Below in conjunction with specific embodiment the present invention is further specified:
Embodiment one
50 of selected red plam weevil pupas, under 28 ℃ of conditions, 49 of emergence adults, with the red plam weevil adult in the ratio of male and female than 1: 1; Packing into has in the vial of 20% hydromel, and every bottled 1 pair of adult laid eggs after 1-6 days, laid eggs 430; 12 days ovum phases, ovum routine disinfection (soaked 1 hour with 10% formalin solution earlier, clean with flushing with clean water then, drying in the shade gets final product); Under 28 ℃ of conditions, 412 of hatching larvas, artificial feed divide to install in the red plam weevil larva raising container by prescription stirring, mixing; To go out to incubate larva and insert (1 of access goes out to incubate larva in every container), and put into healthy supporting between worm and raise, and look the every interval of feed situation certain hour and change the interpolation feed, larva becomes to live 321 after 15 days; Cocoon after 50 days 268, cocoon is taken out deposit separately, aftershaft changed into 257 of worms in 35 days.
Embodiment two
40 of selected red plam weevil pupas, under 28 ℃ of conditions, 38 of emergence adults, with the red plam weevil adult in the ratio of male and female than 1: 1; Packing into has in the vial of 20% hydromel, and every bottled 1 pair of adult laid eggs after 1-6 days, laid eggs 450; 1-2 days ovum phase, ovum routine disinfection (soaked 1 hour with 10% formalin solution earlier, clean with flushing with clean water then, drying in the shade gets final product); Under 28 ℃ of conditions, 428 of hatching larvas, artificial feed divide to install in the red plam weevil larva raising container by prescription stirring, mixing; To go out to incubate larva and insert (1 of access goes out to incubate larva in every container), and put into healthy supporting between worm and raise, and look the every interval of feed situation certain hour and change the interpolation feed, larva becomes to live 299 after 18 days; Cocoon after 48 days 185, cocoon is taken out deposit separately, aftershaft changed into 171 of worms in 32 days.
Embodiment three
60 of selected red plam weevil pupas, under 28 ℃ of conditions, 58 of emergence adults, with the red plam weevil adult in the ratio of male and female than 1: 1; Packing into has in the vial of 20% hydromel, and every bottled 1 pair of adult (cotton balls that will dip in 20% hydromel hangs in the vial, supplies it to get food and lay eggs) laid eggs after 1-6 days; Lay eggs 640,1-2 days ovum phase, the ovum routine disinfection (soaked 1 hour with 10% formalin solution earlier, clean with flushing with clean water then; Dry in the shade and get final product), under 28 ℃ of conditions, 621 of hatching larvas; Artificial feed divide to install in the red plam weevil larva raising container by prescription stirring, mixing, will go out to incubate larva and insert (insert 1 in every container and go out to incubate larva); Put into healthy supporting between worm and raise, look the every interval of feed situation certain hour and change the interpolation feed, larva becomes to live 416 after 17 days; Cocoon after 52 days 389, cocoon is taken out deposit separately, aftershaft changed into 371 of worms in 42 days.
Embodiment four
70 of selected red plam weevil pupas, under 28 ℃ of conditions, 67 of emergence adults, with the red plam weevil adult in the ratio of male and female than 1: 1; Packing into has in the vial of 20% hydromel, and every bottled 1 pair of adult (gauze that will dip in 20% hydromel hangs in the vial, supplies it to get food and lay eggs) laid eggs after 1-6 days; Lay eggs 690,1-2 days ovum phase, the ovum routine disinfection (soaked 1 hour with 10% formalin solution earlier, clean with flushing with clean water then; Dry in the shade and get final product), under 28 ℃ of conditions, 632 of hatching larvas; Artificial feed divide to install in the red plam weevil larva raising container by prescription stirring, mixing, will go out to incubate larva and insert (insert 1 in every container and go out to incubate larva); Put into healthy supporting between worm and raise, look the every interval of feed situation certain hour and change the interpolation feed, larva becomes to live 372 after 15 days; Cocoon after 46 days 329, cocoon is taken out deposit separately, aftershaft changed into 319 of worms in 38 days.
Claims (4)
1. the raising method of a red plam weevil experimental population is characterized in that, its concrete steps are following:
(1) red plam weevil pupa that will be to be raised is 25～28 ℃ in temperature, and adult sprouts wings under the condition of humidity 65～85%; Adult after will sprouting wings is then put into the container that has 20% hydromel for one group respectively by every pair of adult and carries out mate and oviposit; With hatching larva behind the ovum routine disinfection;
(2) the larva access of hatching is equipped with in the red plam weevil larva raising container of red plam weevil larva meridic diet, puts into healthy supporting between worm and raise, the allotment meridic diet is 5～8 times in whole larva raising process, and raising is until larvae pupation; Said meridic diet is to be processed through stirring and evenly mixing by coconut leaf powder, bagasse, oat, corn flour, legumin, glucose, sucrose, agar, yeast, Webster salt, cholesterol, ascorbic acid, potassium sorbate and water, and wherein each component is by weight: coconut leaf powder 50, bagasse 30, oat 20, corn flour 10, legumin 15, glucose 10, sucrose 10, agar 10, yeast 5, Webster salt 2, cholesterol 1, ascorbic acid 1, potassium sorbate 1, water 400; The cleanliness factor that said health is supported between worm is thousand grades, and temperature control is at 27～29 ℃, and control is wet 65～85%.
2. raising method according to claim 1 is characterized in that: in the adult mate and oviposit period, adding little group, to dip in full mass fraction be the adsorbate of the 20% hydromel adult of feeding.
3. raising method according to claim 2 is characterized in that: described adsorbate is cotton or gauze.
4. raising method according to claim 1 is characterized in that: when allocating meridic diet the last time, in feed, add coir, auxiliary mature larva is done cocoon.
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| CN2011100347687A CN102124999B (en) | 2011-01-24 | 2011-01-24 | Method for raising experimental population of Rhynchophorus ferrugineus |
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| CN103431160B (en) * | 2013-08-01 | 2014-11-12 | 福建农林大学 | Semi-artificial feed of red weevil, and preparation method of semi-artificial feed |
| CN106616070B (en) * | 2016-09-19 | 2020-06-05 | 云南农业大学 | Artificial feed for Chinese chestnut weevil, breeding method and application of aged larva or pupa |
| CN107667987B (en) * | 2017-11-20 | 2020-06-09 | 福建农林大学 | Method for creating intestinal sterile rhynchophorus ferrugineus larva experimental model |
| CN112293616A (en) * | 2020-10-22 | 2021-02-02 | 茅台学院 | Method for preparing feed for larva of rhynchophorus ferrugineus by using rice bran |
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| CN101584420B (en) * | 2009-06-10 | 2011-11-23 | 中国热带农业科学院环境与植物保护研究所 | Brontispa longissima larva artificial feed and preparation method |
| CN101703172B (en) * | 2009-11-09 | 2012-05-23 | 中国热带农业科学院椰子研究所 | Semi-artificial feed for larva of rhynchophorus ferrugineus |
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