CN102499189B - Method for subculturing Ectropis obliqua hypulina Wehrli indoor with fresh leaves - Google Patents
Method for subculturing Ectropis obliqua hypulina Wehrli indoor with fresh leaves Download PDFInfo
- Publication number
- CN102499189B CN102499189B CN 201110376275 CN201110376275A CN102499189B CN 102499189 B CN102499189 B CN 102499189B CN 201110376275 CN201110376275 CN 201110376275 CN 201110376275 A CN201110376275 A CN 201110376275A CN 102499189 B CN102499189 B CN 102499189B
- Authority
- CN
- China
- Prior art keywords
- sand
- tea
- larva
- tree branch
- tea tree
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Cultivation Of Plants (AREA)
Abstract
The invention provides a method for subculturing Ectropis oblique hypulina Wehrli indoor with fresh leaves. The method comprises the following steps of: putting disinfected sand into a raising container, and injecting water; keeping the reasonable water quantity of the sand; inserting a tea tree branch into the sand; placing an Ectropis oblique hypulina Wehrli larva collected in the field onto the tea tree branch; covering the raising container with a silk curtain of 180-200 meshes; enabling the Ectropis oblique hypulina Wehrli larva to arbitrarily take food, and adding or replacing the tea tree branch according to the consumption situation of the tea leaves; in the old and mature larva pupating process, reducing water content of the sand; in the process of feathering the pupa into an adult, adding or replacing the tea tree branch; after an egg is hatched, adding a tender tea tree branch; and repeating the abovementioned steps, and successively circulating to achieve the purpose of subculturing the Ectropis oblique hypulina Wehrli indoor with the fresh leaves. The method has the advantages of low cost, easily obtainable materials, high larva survival rate and good larva growth and development condition.
Description
Technical field
The present invention relates to the raising method of a kind of tea geometrid, specifically, related to the indoor bright leaf subculture raising method of a kind of tea geometrid.
Background technology
Tea geometrid (Ectropis obliqua hypulina Wehrli) belongs to Lepidoptera geometer section insect, is commonly called as arch arch worm, hogback worm, hang spring worm, bridging worm etc.So long as the zone that has tea tree to distribute, there is the generation of tea geometrid in the capital, in Zhejiang, all there is generation in province such as Jiangsu, Anhui, Jiangxi, Hubei, Hunan, Sichuan, Guangdong, Henan, it is the most serious a kind of insect of causing harm on the tea tree, the big generation gnawed a light with the full wafer tea place often, shape is as burning, to the Tea Production influence greatly.Algebraically is many, generation overlap weighs, reproduction speed is fast, diffusivity is strong because this worm is taken place, and is easy to outburst and causes disaster.It should be noted that especially, many document announcement tea geometrids have all produced pesticide resistance in various degree to multiple insecticide, under chemical control at present was still this realistic situation of important means in the tea geometrid comprehensive regulation, the numerous and production of the expansion of the monitoring for resistance of tea geometrid, insecticide Toxicity Determination, insecticide field control effectiveness test, tea geometrid virus formulation etc. all needed a large amount of uniform standard worm sources.
In recent years, many bughunters are exploring the indoor feeding method of tea geometrid, and what representative was wherein relatively arranged is artificial feed prescription and water culture; FOREST PROTECT INST CHINA FORE in 2005, Zhejiang Academy of Agricultural Science in 2007 disclose preparation and the feeding method patent of tea geometrid artificial feed respectively; but have that the artificial feed cost is higher, the larva survival rate has only about 70%, raise back worm kind easily degenerate, go through phase cripetura or increase, be not as active as deficiencies such as open-air population; can not reaction field the essential characteristic of outer population, tea geometrid is extensive use in raising few.Water culture has increased the drowned dead probability of larva and tea geometrid in transfer process, inevitably can increase and to lose or dead phenomenon, relatively takes a lot of work simultaneously, time-consuming, effort.
In order to solve the problem of above existence, people are seeking a kind of desirable tea geometrid rearing method always.
Summary of the invention
The objective of the invention is at the deficiencies in the prior art, thus provide a kind of with low cost, draw materials easily, larva survival rate height, the indoor bright leaf subculture raising method of the well-developed tea geometrid of larval growth.
To achieve these goals, the technical solution adopted in the present invention is: the indoor bright leaf subculture raising method of a kind of tea geometrid, and this method may further comprise the steps:
Step 1, the training of branch indoor sand: the sand after will sterilizing is packed into and is raised in the container, injects water in described raising container, and the maintenance sand reaches saturated water amount and the sand top layer does not have open fire, and the tea tree branch that will newly gather inserts in the described sand again;
Step 2, connect worm: the tea geometrid larva of field acquisition is linked on the tealeaves blade of described tea tree branch; Adopt 180-200 purpose yarn account again, described raising container is shrouded;
Step 3, foster worm: appoint a tea geometrid larva arbitrarily to get food, got the food situation according to tealeaves, the tea tree branch in interpolation in good time or the replacing sand;
Step 4, pupate: mature larva pupates to reduce the sand contained humidity and keep sand moistening in the process;
Step 5, emergence: after pupa turns into adult, adult mating in described yarn account, in this process, the tea tree branch in interpolation in time or the replacing sand is so that adult lays eggs at fresh tea tree branch;
Step 6, hatching: after ovum is hatched into tea geometrid larva of future generation, can in sand, add tenderer tea tree branch so that low instar larvae is edible;
Step 7, repeating step 3-6, circulation successively realizes the indoor bright leaf subculture raising of tea geometrid.
Based on above-mentioned, in step 1, the sand after the sterilization refer to sand in limewash, soak eluriate after 3-5 hours clean.
Based on above-mentioned, in step 2, the tea geometrid larva of field acquisition refers to not execute the tea geometrid larva that the tea tree of agricultural chemicals is gathered in half a year at least, and this tea geometrid larva is the length of time and individual tea geometrid larva of the same size.
Based on above-mentioned, in step 4, when mature larva is about to pupate, earlier sand top layer and one in worm excrement are cleaned out; Clean out, sand is carried out disinfection again, sterilization method is: sand was soaked in limewash 3-5 hours, rinse well and get final product.
Based on above-mentioned, it is the middle sand of 0.3--0.5 millimeter that described sand is selected particle diameter for use, and the sand height is the 1/2--2/3 that raises container height.
Based on above-mentioned, described tea tree branch is long to be 40-60cm, and the degree of depth that described tea tree branch inserts described sand is 5-10cm, and described tea tree branch inserts the part of described sand will remove blade.
The relative prior art of the present invention has outstanding substantive distinguishing features and marked improvement, and specifically, the indoor bright leaf subculture raising method of this tea geometrid has the following advantages:
1, larval mortality is low: be sand in the used raising container of this method, do not have open fire above the sand bed, therefore, even larva falls into and raises the phenomenon that container do not have entry death yet and take place; It is fresh that the tea tree branch keeps always, do not have rotten, smelly phenomenon and take place, and reduced the probability that larva catches an illness; Compare with water culture, obviously reduced lethality.
2, adopt this raising method, the whole generation of tea geometrid all can finish in a raising container, need not shift the raising container, has reduced the phenomenon of losing or dying unexpectedly and has taken place.
3, this raising method is compared with water culture, saved artificially collect behind the pupa imbed it in soil, adult regathers ovum after laying eggs, treat to receive after the ovum hatching process of tea tree branch again; According to test, enough moistening as long as sand bed keeps, the tea tree branch can keep 8--12 days fresh, and change 4 tea tree branches approximately and get final product the whole history of life of tea geometrid; Adopt this raising method saving of labor, save time, laborsaving.
4, draw materials easily, cost is low: so long as watertight container, all can use as containers such as Plastic Drum, plastic basin, antistaling boxes, raising instruments such as professional dependent insect cage, box for feeding pests need not be arranged, the tea tree branch can be gathered from the field, therefore, remove outside the dress husky container and nylon gauze, other just without any need for spending.
5, the number of animals raised is big, and is workable: as the plastic containers of a diameter 37cm, high 36 cm, each branch is the spacing of 2--3cm at interval, and each bucket can be inserted 150--230 branch, can raise 500--800 larva, manually can raise ten thousand larvas of 2-3 for one.
6, the core of this raising method is to adopt the husky training of tea tree branch, bright leaf subculture to raise the worm source of each worm attitude of a large amount of tea geometrids, compare with the artificial feed raising, this method is with low cost, draw materials easily, larval growth physically well develops, lethality is low, near the life habit of open-air tea geometrid.
Embodiment
Below by embodiment, technical scheme of the present invention is described in further detail.
The indoor bright leaf subculture raising method of a kind of tea geometrid, this method may further comprise the steps:
Step 1, the training of branch indoor sand: the sand after will sterilizing is packed into and is raised in the container, injects water in described raising container, and the maintenance sand reaches saturated water amount and the sand top layer does not have open fire, and the tea tree branch that will newly gather inserts in the described sand again; Wherein, the sand after the sterilization refer to sand in limewash, soak eluriate after 3-5 hours clean; It is the middle sand of 0.3--0.5 millimeter that described sand is selected particle diameter for use, and the sand height is the 1/2--2/3 that raises container height; Raise container and can adopt Plastic Drum, plastic basin, antistaling box etc., so long as watertight container all can use; What present embodiment adopted is that diameter is that 37cm, height are the Plastic Drum of 36cm, and a Plastic Drum can be raised 500--800 larva.
Step 2, connect worm: the tea geometrid larva of field acquisition is linked on the tealeaves blade of described tea tree branch and lets alone to get food; Adopt the red nylon yarn account of 180-200 purpose again, described raising container is shrouded, the yarn account can be prevented the low instar larvae escape; The tea geometrid larva of field acquisition refers to not execute the tea geometrid larva that the tea tree of agricultural chemicals is gathered in half a year at least, this tea geometrid larva is healthy, active, length of time and individual tea geometrid larva of the same size, and present embodiment is selected 5 instar larvaes for use.
Step 3, foster worm: appoint a tea geometrid larva arbitrarily to get food, got the food situation according to tealeaves, the tea tree branch in interpolation in good time or the replacing sand.
Step 4, pupate: when mature larva is about to pupate, earlier sand top layer and one in worm excrement are cleaned out, sand is carried out disinfection again, sterilization method is: sand was soaked in limewash 3-5 hours, rinse well and get final product; Can pierce in the sand voluntarily when mature larva is pupated and pupate, mature larva is pupated in the process, reduces the sand contained humidity and keeps sand moistening.
Step 5, sprout wings: after pupa turned into adult, adult mating in described yarn account in this process, was in time added or is changed tea tree branch in the sand, so that adult lays eggs at fresh tea tree branch, guaranteed ovum required humidity in growth and development process.
Step 6, hatching: after ovum is hatched into tea geometrid larva of future generation, can in sand, add tenderer tea tree branch so that low instar larvae is edible; Because low instar larvae has tender characteristic, therefore, larva was preferably gathered young sprout branch tenderer on the tea tree for larval feeding before three ages, and no matter old shoot all can after three ages; In addition, the yarn account can be prevented the low instar larvae escape.
Step 7, repeating step 3-6, circulation successively realizes the indoor bright leaf subculture raising of tea geometrid.
Based on above-mentioned, described tea tree branch is that 1-2 gives birth to, vigorous, the anosis worm of growth is caused harm, long for 40-60cm, do not execute the tea tree branch of agricultural chemicals in half a year at least; The degree of depth that described tea tree branch inserts described sand is 5-10cm; Described tea tree branch inserts the part of described sand will remove blade, in order to avoid the blade of imbedding in the sand rots, produces pathogen, and it is susceptible to cause larva; Sand will keep moistening always, makes the moisture that blade is rising and breathing is scattered and disappeared of tea tree branch, can in time be replenished by the moisture that tea tree branch bottom stem stalk absorbs in the sand; Adopt the method for present embodiment to raise tea geometrid, the tea tree branch can keep 8-12 days fresh, generally needs to change 4 tea tree branches the whole history of life of tea geometrid and gets final product, and can save working time and recruitment greatly.
The process of growing and the temperature of tea geometrid have very big relation: general 3～5d of adult life-span; The ovum phase, 10～15d during 15～20 ℃ of mean temperature of air, 4～5d in the time of 28～30 ℃; 20～25d when larva, 15～20 ℃ of mean temperature of air, 12～15d in the time of 28～30 ℃; When pupa, 20 ℃ of mean temperature of air about 10d, 5～7d in the time of 30 ℃.
Comparative trial:
Test period: on September 25,12 days to 2011 August in 2011;
Test site: plant protection research department insect laboratory of the Xinyang institute of agricultural sciences, carry out the indoor bright leaf subculture of tea geometrid and raise; Ecological tea plantation, Xinyang institute of agricultural sciences tealeaves test garden is carried out the tea geometrid field and is shrouded raising;
Raising method: according to above-described embodiment, carry out the indoor bright leaf subculture of tea geometrid and raise; The tea geometrid field shrouds that to raise be to shroud 2 meters long with 180 purpose nylon screen windows with 1.5 meters tea of 2 line width are capable, and 150 tea geometrid 5 instar larvaes are evenly disperseed to insert tea tree, lets alone to get food under field conditions (factors), breeds.
Table 1: indoor bright leaf subculture is raised and the open-air comparing result of raising tea geometrid that shrouds
| Content | Indoor bright leaf subculture is raised | Raising is shrouded in the field |
| Larvae development go through the phase (my god) | 15.2 | 15.8 |
| Survival rate of larvae (%) | 95.6 | 87.5 |
| Pupa development go through the phase (my god) | 7.2 | 6.8 |
| 10 pupas heavy (gram) | 1.41 | 1.49 |
| Adult eclosion rate (%) | 92.3 | 90.4 |
| The adult egg laying amount (grain/to) | 201.4 | 204.2 |
| Egg hatching rate (%) | 90.1 | 74.6 |
This test was adopted indoor bright leaf subculture raising and field to shroud respectively and is raised generation of tea geometrid from September 25,12 days to 2011 August in 2011; August 12, gather from the field 5 age 150 of tea geometrid larvas, beginning to carry out indoor bright leaf subculture raises, September 10, statistics had 10162 of larvas approximately, the tea geometrid that this method is raised is gone through the phase except larvae development and shrouds raising method shortening 0.6 day than the field, pupa anharmonic ratio field is shrouded beyond few 0.08 gram of raising method, egg laying amount shrouds raising method than the field and lacks outside 2.8, other as survival rate of larvae, the adult eclosion rate, indexs such as egg hatching rate all are higher than the field and shroud raising method, the larva stalwartness of cultivating, vivaciously, it is good to grow, life habit near open-air tea geometrid, the every research that can be tea geometrid provides a large amount of uniform standard worm sources, has reached the practicability requirement.
Should be noted that at last: above embodiment is only in order to illustrate that technical scheme of the present invention is not intended to limit; Although with reference to preferred embodiment the present invention is had been described in detail, those of ordinary skill in the field are to be understood that: still can make amendment or the part technical characterictic is equal to replacement the specific embodiment of the present invention; And not breaking away from the spirit of technical solution of the present invention, it all should be encompassed in the middle of the technical scheme scope that the present invention asks for protection.
Claims (7)
1. indoor bright leaf subculture raising method of tea geometrid, it is characterized in that: this method may further comprise the steps: step 1, the training of branch indoor sand: the sand after will sterilizing is packed into and is raised in the container, in described raising container, inject water, the maintenance sand reaches saturated water amount and the sand top layer does not have open fire, and the tea tree branch that will newly gather inserts in the described sand again; Step 2, connect worm: the tea geometrid larva of field acquisition is linked on the tealeaves blade of described tea tree branch; Adopt 180-200 purpose yarn account again, described raising container is shrouded; Step 3, foster worm: appoint a tea geometrid larva arbitrarily to get food, got the food situation according to tealeaves, the tea tree branch in interpolation in good time or the replacing sand; Step 4, pupate: mature larva pupates to reduce the sand contained humidity and keep sand moistening in the process; Step 5, emergence: after pupa turns into adult, adult mating in described yarn account, in this process, the tea tree branch in interpolation in time or the replacing sand is so that adult lays eggs at fresh tea tree branch; Step 6, hatching: after ovum is hatched into tea geometrid larva of future generation, can in sand, add tenderer tea tree branch so that low instar larvae is edible; Step 7, repeating step 3-6, circulation successively realizes the indoor bright leaf subculture raising of tea geometrid.
2. the indoor bright leaf subculture raising method of tea geometrid according to claim 1 is characterized in that: in step 1, the sand after the sterilization refer to sand in limewash, soak eluriate after 3-5 hours clean.
3. the indoor bright leaf subculture raising method of tea geometrid according to claim 1 and 2, it is characterized in that: in step 2, the tea geometrid larva of field acquisition refers to not execute the tea geometrid larva that the tea tree of agricultural chemicals is gathered in half a year at least, this tea geometrid larva is the length of time and individual tea geometrid larva of the same size.
4. the indoor bright leaf subculture raising method of tea geometrid according to claim 3 is characterized in that: in step 4, when mature larva is about to pupate, earlier sand top layer and one in worm excrement are cleaned out.
5. the indoor bright leaf subculture raising method of tea geometrid according to claim 4, it is characterized in that: clean out, sand is carried out disinfection again, sterilization method is: sand was soaked in limewash 3-5 hours, rinse well and get final product.
6. the indoor bright leaf subculture raising method of tea geometrid according to claim 4, it is characterized in that: it is the middle sand of 0.3--0.5 millimeter that described sand is selected particle diameter for use, the sand height is the 1/2--2/3 that raises container height.
7. the indoor bright leaf subculture raising method of tea geometrid according to claim 4, it is characterized in that: the long 40-60cm of being of described tea tree branch, the degree of depth that described tea tree branch inserts described sand is 5-10cm, and described tea tree branch inserts the part of described sand will remove blade.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110376275 CN102499189B (en) | 2011-11-24 | 2011-11-24 | Method for subculturing Ectropis obliqua hypulina Wehrli indoor with fresh leaves |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110376275 CN102499189B (en) | 2011-11-24 | 2011-11-24 | Method for subculturing Ectropis obliqua hypulina Wehrli indoor with fresh leaves |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102499189A CN102499189A (en) | 2012-06-20 |
| CN102499189B true CN102499189B (en) | 2013-08-14 |
Family
ID=46211371
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201110376275 Expired - Fee Related CN102499189B (en) | 2011-11-24 | 2011-11-24 | Method for subculturing Ectropis obliqua hypulina Wehrli indoor with fresh leaves |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102499189B (en) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102919202A (en) * | 2012-11-22 | 2013-02-13 | 广西壮族自治区林业科学研究院 | Indoor artificial breeding method of buzura suppressaria |
| CN103563856B (en) * | 2013-10-28 | 2015-09-30 | 中国农业科学院茶叶研究所 | The collection of ovum and store method in a kind of tea geometrid mass rearing |
| CN106172243B (en) * | 2016-07-11 | 2019-01-11 | 安徽农业大学 | A method of being simple and efficient preparation bunch caterpillar sand |
| CN108935347B (en) * | 2018-10-12 | 2021-04-09 | 信阳农林学院 | Breeding method of griseofulvin |
| CN109526890A (en) * | 2019-01-14 | 2019-03-29 | 福建省农业科学院植物保护研究所 | A kind of method of interior mass rearing tea geometrid |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100539870C (en) * | 2007-04-29 | 2009-09-16 | 浙江省农业科学院 | The man-made feeds of a kind of tea geometrid, the method for breeding of its preparation and tea geometrid |
-
2011
- 2011-11-24 CN CN 201110376275 patent/CN102499189B/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| CN102499189A (en) | 2012-06-20 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101228853B (en) | Artificial feeding and culture transfer of monochamus alternatus hope | |
| CN1954669B (en) | Artificial feeding and generation method of Ectropis oblique hypulin W. and artificial feed for its larva | |
| CN101796940A (en) | Super flour weevil culture technology | |
| CN206699188U (en) | A kind of Tephritidae insect breeding apparatus | |
| CN103782969B (en) | A kind of method of indoor propagation Trichogramma pintoi and rice moth culturing feed | |
| CN102499189B (en) | Method for subculturing Ectropis obliqua hypulina Wehrli indoor with fresh leaves | |
| CN103155906A (en) | Aphidoletes aphidimyza mass reproduction technology and application technology | |
| CN102499188B (en) | Agasicles hygrophila experimental population raising method | |
| CN106508794A (en) | Toad early-breeding high-yield three-dimensional factory cultivation method and application | |
| CN102113471B (en) | Artificial rearing method of larvae of beet armyworm | |
| CN113080147B (en) | Method for preventing and treating yellow leaf borers by trichogramma | |
| CN105532587B (en) | Mediterranean flour moth method for breeding and application | |
| CN104957107B (en) | A kind of method of interior raising Propylaea japonica | |
| CN106857414A (en) | The mass rearing method of prodenia litura | |
| CN106804531B (en) | Comprehensive breeding method for cuora flavomarginata from parent cuora to adult cuora flavomarginata | |
| CN107581159A (en) | A kind of figured cloth moths attracted by lamplight natural enemy hangs the artificial fecundation method of cocoon honeybee | |
| CN106900538A (en) | A kind of method that the onion production of hybrid seeds is carried out using fly | |
| CN103858827A (en) | Method for carrying out indoor large-scale diadegma semiclausum hellen breeding | |
| CN108935341B (en) | Method for raising cabbage butterfly velvet cocoon bee | |
| CN112385608A (en) | Breeding method of lugworm | |
| CN106719408A (en) | Cuora flavomarginate becomes more meticulous comprehensive breeding method | |
| CN106172248A (en) | A kind of expanding propagation method of Japan food a red-spotted lizard aphid chalcid fly | |
| CN103766288B (en) | The a large amount of propagation method of moth knitted by coconut | |
| CN104604809A (en) | Biological control method using encarsia formosa for controlling tobacco whitefly | |
| CN103478080B (en) | The genus rejuvenation cultural method of a kind of artificial propagation Chinese caterpillar fungus host insect |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20130814 Termination date: 20141124 |
|
| EXPY | Termination of patent right or utility model |