CN103563856B - The collection of ovum and store method in a kind of tea geometrid mass rearing - Google Patents
The collection of ovum and store method in a kind of tea geometrid mass rearing Download PDFInfo
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- CN103563856B CN103563856B CN201310514825.0A CN201310514825A CN103563856B CN 103563856 B CN103563856 B CN 103563856B CN 201310514825 A CN201310514825 A CN 201310514825A CN 103563856 B CN103563856 B CN 103563856B
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Abstract
In tea geometrid mass rearing, the collection of ovum and a store method, belong to technical field of insect feeding.It comprises the following steps: 1) tea geometrid larvae mass rearing; 2) collection of tea geometrid pupa; 3) adults of ectropis obliqua is laid eggs and the collection of ovum; 4) refrigeration of tea geometrid ovum.The present invention has following beneficial effect: one is get that ovum is fast and simple, efficiency is high, particularly suitable in tea geometrid mass rearing, present invention omits adult pairing in current methods, do the steps such as bar of laying eggs, adult is concentrated and raises, directly lay eggs on blank sheet of paper, get ovum easy to operate, time saving and energy saving, utensil used is few simultaneously, saves cultured space; Two is adopt the method for moisturizing in refrigerating bottle to extend ovum cold preservation time, ovum storage life of the present invention extended to 20 days in 15 days from the past, cold preserving method storage life extends 5 days than ever, can increase the raising batch during tea geometrid mass rearing, improves and totally raises quantity.
Description
Technical field
The invention belongs to technical field of insect feeding, be specifically related to collection and the store method of ovum in a kind of tea geometrid mass rearing.
Background technology
Tea geometrid spreads all over the primary pest that China respectively produces tea province.Tea geometrid mass rearing is the basis of carrying out tea geometrid virus breeding and multiple experimental study.The Collection and preservation of ovum affects scale and the period of mass rearing.In current tea geometrid mass rearing, the collection method of ovum gets ovum method for bar of laying eggs, namely after adult eclosion, 1-2 is placed in 500mL open mouth glass Cans to female male imago, in bottle, places 1-3 bar (long 10cm, wide 2cm are converted into by three-ply paper, order conjunction, the crannied paper slip of tool with stapler) of laying eggs simultaneously.After Adult worms producting eggs, taking-up is laid eggs, and bar is concentrated to be placed in Cans.The method mainly comprise making lay eggs bar, adult pairing, get bar three steps of laying eggs, very time-consumingly take a lot of work, especially adult pairing, large in adult quantity, time arrival in emergence peak, the workman of a skilled operation only matches and just need spend half a day many time.And utensil used is many, the space taken is large.To raise out 1000 adults, adult pairing at least needs Cans 250, bar 500 of laying eggs.In addition, in matching operation, adult very easily runs fast loss, also likely because not matching in time, affects egg laying amount.The store method of current ovum is deepfreeze method, and the bar of laying eggs being about to band ovum is placed in glass preserving jar, and bottleneck is tightened and is placed on 4 DEG C of refrigerator cold-storages.The method holding time mostly is 15 days most, sharply reduces more than the incubation rate of 15 days ovum.The stored refrigerated of ovum makes fed-batch become possibility, the length of holding time determine raising batch, batch number then have influence on the size of raising total amount.Calculate to raise a collection of worm every 7 days, the refrigeration phase is that within 15 days, then to raise batch be 2 times.If storage life extends to 20 days, then raising batch can bring up to 3 times.Therefore, find a kind of egg-fetching method of high-efficient simple and the longer store method of storage life, to raising insect feeding efficiency, reduce recruitment, the total number improving tea geometrid mass rearing has great importance.
Summary of the invention
For prior art Problems existing, the object of the invention is to design provides the collection of ovum and the technical scheme of store method in a kind of tea geometrid mass rearing, and it is easy, quick, efficient that the method not only gets ovum, and can extend ovum storage life.
The collection of ovum and store method in described a kind of tea geometrid mass rearing, is characterized in that comprising the following steps:
1) tea geometrid larvae mass rearing
Tea geometrid newly hatched larvae is first raised with tender tealeaves in 500mL wide mouth glass bottle, method of transplanting is adopted to raise after 2-3 days again, namely from field clip tea branch, bunchy is inserted in the wide-mouth bottle that 500mL is filled with water, and wide-mouth bottle is placed in large size plastic basin, every plastic basin places some wide-mouth bottles, then the larva raised, residual leaf is received on tea branch and is raised;
2) collection of tea geometrid pupa
After larva all pupates, be collected in by pupa in Cans, in Cans, place 1-2 Zhang Lao tealeaves, bottleneck blank sheet of paper is tightened simultaneously, and covers sheet glass to keep certain humidity;
3) adults of ectropis obliqua is laid eggs and the collection of ovum
When adult starts to sprout wings, the Cans that pupa is housed are placed in dependent insect cage, open bottle cap, allow tea geometrid fly out, pangamy in cage, described dependent insect cage encloses the cuboid cage body of structure by gauze, the gauze of dependent insect cage is provided with the blank sheet of paper for laying eggs, and plays timing every day next day and takes off blank sheet of paper, collect the ovum on blank sheet of paper, and concentrate in loading small beaker, get after ovum terminates and blank sheet of paper is retightened on gauze;
4) refrigeration of tea geometrid ovum
The small beaker that ovum is housed is placed in Cans by every day, and in Cans, place 1-2 Zhang Lao tealeaves, first with blank sheet of paper and rubber band sealing, then tightens bottleneck with plastic film, puts into 4 DEG C of refrigerator refrigerations.
In described a kind of tea geometrid mass rearing, the collection of ovum and store method, is characterized in that described step 1) and 2) in rearing conditions be constant temperature 23.5 ± 0.5 DEG C, natural lighting.
In described a kind of tea geometrid mass rearing, the collection of ovum and store method, is characterized in that described step 2) in place 200-250 pupa in Cans.
In described a kind of tea geometrid mass rearing, the collection of ovum and store method, is characterized in that placing 800-1250 pupa in dependent insect cage in described step 3).
The collection of ovum and store method in described a kind of tea geometrid mass rearing, is characterized in that placing the cotton ball that 2-3 is filled 5% honeybee water in described step 3) in dependent insect cage.
Compared with prior art, the present invention has following beneficial effect: one be get ovum fast and simple, improve efficiency, particularly suitable in tea geometrid mass rearing.Present invention omits adult pairing in current methods, do the steps such as bar of laying eggs, concentrated by adult and raise, directly lay eggs on blank sheet of paper, get ovum easy to operate, time saving and energy saving, utensil used is few simultaneously, saves cultured space; Two is adopt the method for moisturizing refrigeration in bottle to extend ovum cold preservation time, ovum storage life of the present invention extended to 20 days in 15 days from the past, cold preserving method storage life extends 5 days than ever, can increase the raising batch during tea geometrid mass rearing, improves and totally raises quantity.
Embodiment
The present invention is further illustrated below in conjunction with embodiment.
Embodiment 1:
Get 500mL open mouth glass Cans, put into 200-300 grain ovum (available little writing brush picking) for every bottle.The tender tip of tea tree is dropped into, with blank sheet of paper and rubber band sealing after larvae hatch.1st day paper and bottleneck must be combined closely, and escape in case newly hatched larvae is climbed.After feed is gone by food mostly, new feedstuff need be added in time.Raising after 2-3 days adopts method of transplanting to raise.From the tealeaves branch of the tender tip of field clip band, in the wide-mouth bottle that bunchy insertion 500mL is filled with water.3-4 bottle tea branch is drawn close in the plastic basin being placed on diameter 60cm, form a little tea fluffy.The larva (6 bottles) of resting in worm bottle move to tea fluffy on, let alone to spread on tea is fluffy to take food.Add in good time according to taking food situation or change tea branch after 4 ages.Dropping when larva is aging drops pupates in basin.
After larva all pupates, with different object stainless steel sift by screenings such as pupa, worm excrement and residual leaves, pupa is collected in Cans.About put 200-250 pupa for every bottle, in bottle, place 1-2 Zhang Lao tealeaves, bottleneck blank sheet of paper is tightened, and covers sheet glass to keep certain humidity simultaneously.The rearing conditions of above-mentioned larva and pupa is constant temperature 23.5 ± 0.5 DEG C, natural lighting.
Start, after emergence, to be placed on by the Cans that pupa is housed in dependent insect cage (long 25cm × wide 25cm × high 45cm, plastic gauze) until adult, 4-5 bottle (an about 800-1250 pupa) placed by every cage.Open bottle cap, and in cage, place the cotton ball that 2-3 is filled 5% hydromel, appoint adult pangamy in cage, lay eggs.Meanwhile, 3 veils that blank sheet of paper (more smaller than gauze) is fixed on dependent insect cage with adhesive tape are respectively online, make it be close to outside gauze, the gauze at dependent insect cage top covers blank sheet of paper, lay eggs for it.Start timing every day next day and take off blank sheet of paper, brush the ovum on lower blank sheet of paper gently with small size writing brush or cut the band ovum scraps of paper, concentrate and load in 10-20mL small beaker, and mark gets the ovum date.Get after ovum terminates and blank sheet of paper is retightened on gauze.
Be placed in Cans (500mL) by what be equipped with ovum at small beaker, in bottle, place 1-2 Zhang Lao tealeaves simultaneously, with blank sheet of paper and rubber band sealing, then tighten bottleneck moisturizing with plastic paper, put into 4 DEG C of refrigerators and refrigerate.Refrigeration ovum can be taken out at any time according to raising need in 20 days to raise.
The collection of ovum and store method in above-mentioned tea geometrid mass rearing, compare with existing method, have two large advantages: it is high that (1) gets ovum efficiency, particularly suitable in tea geometrid mass rearing.The inventive method eliminates in current methods and makes bar of laying eggs, adult pairing, gets the steps such as bar of laying eggs, and adult concentrates raising to lay eggs, and ovum produces on the blank sheet of paper outside dependent insect cage gauze, gets ovum convenient, very time saving and energy saving.And eliminate a large amount of utensils used in current methods, save cultured space.To raise 1000 adults, current methods needs to use Cans 250, bar 500 of laying eggs, and only pairing just must spend 1 experienced operator's time half a day, and the time of getting ovum bar every day also needs 1-2 hour.And the inventive method only needs 1 dependent insect cage and 4-5 Cans, getting the ovum time every day only needs 30 minutes.(2) ovum long shelf-life.The inventive method extends ovum cold preservation time, adopts this method refrigeration, can cold preserving method storage life extend 5 days (table 1) than ever.The store method of ovum was deepfreeze in the past, and the bar of laying eggs being about to band ovum is placed in Cans and is placed in 4 DEG C of refrigerator cold-storages.The method holding time mostly is 15 days most, sharply reduces more than the incubation rate of 15 days ovum.The inventive method adopts low-temperature moisture preservation refrigeration, and the ovum holding time can extend to 20 days.Ovum is after stored refrigerated, and the egg hatching time of taking out on the same day is basically identical, and after the ovum refrigeration that namely same date does not produce, taking out raising on the same day can hatch on the same day.This is very important in mass rearing, and the larval growth making often to criticize raising is consistent, and regularity is high, this to virus breeding and other tests all very favourable.The length of holding time determine raising batch, batch number then have influence on the size of raising total amount.As for propagative viruses, raise a collection of worm calculate every 7 days, the refrigeration phase is that within 15 days, then to raise batch be 2 times, can only breed 2 cycles.If the refrigeration phase extends to 20 days, then raising batch can bring up to 3 times, and namely bring up to 3 breeding cycles, corresponding viral yield can improve 50%.In sum, the present invention can improve insect feeding efficiency, reduces recruitment, improves the total number of tea geometrid mass rearing, to virus breeding and various test very favourable.
Table 1 moisturizing refrigeration is on the impact of tea geometrid refrigeration egg hatching rate (%)
| Process | Refrigerate 15 days | Refrigerate 20 days | Refrigerate 25 days | Refrigerate 30 days |
| Moisturizing refrigerates | 100 a | 100 a | 10 a | 0 |
| Common refrigeration | 93.8 a | 56.3 b | 8.8 a | 0 |
Claims (4)
1. the collection of ovum and a store method in tea geometrid mass rearing, is characterized in that comprising the following steps:
1) tea geometrid larvae mass rearing
Tea geometrid newly hatched larvae is first raised with tender tealeaves in 500mL wide mouth glass bottle, method of transplanting is adopted to raise after 2-3 days again, namely from field clip tea branch, bunchy is inserted in the wide-mouth bottle that 500mL is filled with water, and wide-mouth bottle is placed in large size plastic basin, every plastic basin places some wide-mouth bottles, then the larva raised, residual leaf is received on tea branch and is raised, rearing conditions is constant temperature 23.5 ± 0.5 DEG C, natural lighting;
2) collection of tea geometrid pupa
After larva all pupates, be collected in by pupa in Cans, in Cans, place 1-2 Zhang Lao tealeaves, bottleneck blank sheet of paper is tightened simultaneously, and covers sheet glass to keep certain humidity, and rearing conditions is constant temperature 23.5 ± 0.5 DEG C, natural lighting;
3) adults of ectropis obliqua is laid eggs and the collection of ovum
When adult starts to sprout wings, the Cans that pupa is housed are placed in dependent insect cage, open bottle cap, allow tea geometrid fly out, pangamy in cage, described dependent insect cage encloses the cuboid cage body of structure by gauze, and the gauze of dependent insect cage is provided with the blank sheet of paper for laying eggs, and starts timing every day next day and takes off blank sheet of paper, cut the band ovum scraps of paper, concentrate and load in 10-20mL small beaker, and mark gets the ovum date, gets after ovum terminates and retightens on gauze by blank sheet of paper;
4) refrigeration of tea geometrid ovum
The small beaker that ovum is housed is placed in Cans by every day, and in Cans, place 1-2 Zhang Lao tealeaves, first with blank sheet of paper and rubber band sealing, then tightens bottleneck with plastic film, puts into 4 DEG C of refrigerator refrigerations.
2. the collection of ovum and store method in a kind of tea geometrid mass rearing as claimed in claim 1, is characterized in that described step 2) in place 200-250 pupa in Cans.
3. the collection of ovum and store method in a kind of tea geometrid mass rearing as claimed in claim 1, is characterized in that placing 800-1250 pupa in dependent insect cage in described step 3).
4. the collection of ovum and store method in a kind of tea geometrid mass rearing as claimed in claim 1, is characterized in that placing the cotton ball that 2-3 is filled 5% hydromel in described step 3) in dependent insect cage.
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| CN108935347B (en) * | 2018-10-12 | 2021-04-09 | 信阳农林学院 | Breeding method of griseofulvin |
| CN112514854A (en) * | 2020-11-24 | 2021-03-19 | 赤水山宝生态茶业开发有限公司 | High-quality insect source artificial culture method and culture device thereof |
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