CN102771450A - Brontispa longissima mass propagation method - Google Patents
Brontispa longissima mass propagation method Download PDFInfo
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- CN102771450A CN102771450A CN2012102886111A CN201210288611A CN102771450A CN 102771450 A CN102771450 A CN 102771450A CN 2012102886111 A CN2012102886111 A CN 2012102886111A CN 201210288611 A CN201210288611 A CN 201210288611A CN 102771450 A CN102771450 A CN 102771450A
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Abstract
The invention discloses a brontispa longissima mass propagation method. The brontispa longissima in various states is fed under the conditions of the temperature of 24-27 DEG C, the relative humidity of 75 percent +/- 10 percent and the natural illumination. The method comprises the following steps of: cutting an unfolded coconut tree old leaf into small sections, bundling the sections into a small bundle and putting the bundle into a feeding box by using a rubber band, primarily hatching 500 pairs of brontispa longissima adults, and replacing the leaf once every three days, wherein the brontispa longissima begin to spawn after 10 days, removing the leaves with eggs, and putting the eggs into another feeding box in a centralized mode; hatching the eggs, putting coconut tree core leaves into the feeding box, wherein the primarily hatched larva eat the coconut tree core leaves and grow; feeding the larva with the age of more than two years by using the folded coconut tree old leaves, wherein the larva pupate within 25 days, and the larva are subjected to eclosion to form adult within 6 days; and entering the next-generation feeding and breeding. The coconut tree old leaves are used for feeding the adults and larva with the age of more than two years instead of the core leaves, the cost is reduced, and the brontispa longissima mass propagation method is easy and convenient to operate and practical and can continuously perform mass propagation on the brontispa longissima in various age states in a balanced mode.
Description
Technical field
The present invention relates to the prolific method of a kind of Brontispa longissima.
Background technology
Brontispa longissima Brontispa longissima Gestro is the external pest of China's substantial risk property; Belong to coleoptera (Coleoptera); Chrysomelidae (Chrysomelidae), Chinese translation have the flat leaf beetle of coconut, red chest leaf worm, coconut palm come into leaves first, coconut palm palm fibre flat chrysomelid, the chrysomelid and red chest of coconut bristle is chrysomelid.Brontispa longissima originates in Indonesia, has become the primary pest of Asian-Pacific area palm plant over nearly 30 years.This insect is got food palm plant lobus cardiacus, causes the plant apical cap withered, can cause whole strain dead when serious.2002, Brontispa longissima invasion China Hainan Province, and break out and cause disaster, cause 90% coconut palm to be injured, vast cocoanut tree plantation family income is received appreciable impact.At present, Brontispa longissima has also spread some areas such as spreading to Guangdong, Guangxi and Yunnan except that Hainan the whole province causes disaster.The invasion of this insect has destroyed China's torrid zone ecotope, is threatening China's palm plant related industry.
Brontispa longissima has fast, the destructive strong and big characteristics of difficulty of prevention and cure of breeding.Break out the initial stage this insect, chemical spray is widely used as emergency measure.But Brontispa longissima is hided and in the not expansion leaf of babassu, is caused harm, and medicament is difficult to touch target pest, and tall and big palm plant causes the dispenser difficulty.The biological control Brontispa longissima is a kind of cost-effective measure.Brontispa longissima natural enemy parasitic wasp introduction of use is the important measures of control Brontispa longissima, and there is successful experience in a plurality of countries.China introduces respectively in 2004 that the coconut palm first is cut arteries and veins Ji chalcid fly Asecodes hispinarum and Brontispa longissima is nibbled chalcid fly Tetrastichus brontispae, and expands numerous and application.The coconut palm first is cut the larva parasitic wasp that arteries and veins Ji chalcid fly is a Brontispa longissima, Brontispa longissima 4 instar larvaes that the numerous needs of indoor expansion are a large amount of.Brontispa longissima is nibbled the pupa parasitic wasp that chalcid fly is a Brontispa longissima, the first pupa of Brontispa longissima that the numerous needs of indoor expansion are a large amount of.The research of the biology of Brontispa longissima, ecology and other prophylactico-therapeutic measuress also needs a large amount of worm age consistent Brontispa longissima as trying worm.So a large amount of propagation techniques of Brontispa longissima are the bases of carrying out biological control, also be the basic technology of carrying out other research of Brontispa longissima.
Still have nothing to do both at home and abroad at present in the research report of a large amount of propagating and breeding technologies of Brontispa longissima.The a large amount of breedings of Brontispa longissima at first solve the feed resource problem.Under the natural conditions, how the Brontispa longissima lucifuge does not launch to get in the lobus cardiacus food harm cocoanut tree.Get the cocoanut tree lobus cardiacus as the Brontispa longissima foodstuff if cut, certainly will influence the cocoanut tree growth.Than the cocoanut tree lobus cardiacus, the old Ye Gengyi of the cocoanut tree of expansion obtains, and cost is low, and to the basic not influence of tree vigo(u)r growth.The cocoanut tree Lao Ye that launches is the pinniform leaf that totally cleaves, the Lao Ye that adult cocoanut tree has the 15-20 sheet to launch, and every leaf is removed petiole, has 3 kilograms coconut to can be used for the feeding Brontispa longissima approximately.Under the natural conditions, because of biological habit, the Brontispa longissima lucifuge need be hided and in the cocoanut tree lobus cardiacus that does not launch, got food, and can not get the cocoanut tree Lao Ye that food launches.Research shows, raises Brontispa longissima with the cocoanut tree Lao Ye that launches, and feeding effect is no less than the effect with the raising of cocoanut tree lobus cardiacus, some biological parameter even be superior to raising with the cocoanut tree lobus cardiacus.
That the Brontispa longissima mass rearing must be based upon is simple to operate, on the basis of intensification and high viability, it is consistent that the examination worm that obtains also must physiology.
Summary of the invention
The purpose of this invention is to provide a kind of to almost not injury of Brontispa longissima, low cost, survival rate is high, can obtain a large amount of propagation methods of Brontispa longissima of a large amount of worm unanimities in age.
To achieve these goals, technical scheme of the present invention is: provide a kind of Brontispa longissima a large amount of propagation methods, comprise the steps:
(1) raising of Brontispa longissima adult and bring out it and lay eggs
1. the cocoanut tree Lao Ye that launches is cut into the segment of 150mm, pricks 1 bundle with rubber band, put into insect box for per 50;
2. introduce 1000 Brontispa longissimas in each insect box and just incubate adult, the male and female ratio is 1 ︰ 1;
3. changed primary vane in per 3 days, when changing blade original blade bulk storage is tied up at new blade, the blade that the Brontispa longissima adult can climb to new replacing is automatically tied and is got food and grow;
4. Brontispa longissima begins to lay eggs after 10 days, and ovum originates in blade surface, and 3-5 grain ovum is an extended spread usually, and the blade that will have ovum when at every turn changing blade is chosen, and puts into another insect box with a collection of concentrating, and every box is put 2000 ovum;
(2) raising of ovum hatching and 1 instar larvae
1. ovum began hatching after 4-5 days, and cocoanut tree lobus cardiacus before hatching that 10 left and right sides 150mm are long is sneaked into the blade that has ovum, and newly hatched larvae can be climbed to automatically and get food on the lobus cardiacus;
2. 1 instar larvae phase was 3 days, and the cocoanut tree lobus cardiacus that will have 1 instar larvae after 3 days is put into the insect box that the cocoanut tree Lao Ye of expansion ties, and carries out the raising of larval phase;
(3) management of larval phase raising and pupa
1. 2-3 instar larvae, every box is raised 2000, changes 1 blade in per 3 days, if the dead worm of band mildew is arranged, should choose;
2. 4-5 instar larvae, every box is raised 1000, changes 1 blade in per 3 days, if the dead worm of band mildew is arranged, should choose;
3. larval phase 24-26 days, larvae pupation after 25 days, be 5-7 days pupa time, need not change blade, but will keep ambient humidity; When pupa turned into adult soon, the blade that more renews let it lay eggs, and got into next captive breeding from generation to generation.
In order to satisfy the healthy development of Brontispa longissima, each worm attitude raising condition is 24～27 ℃ of temperature, relative moisture 75% ± 10%, natural lighting.
In order to let Brontispa longissima get the cocoanut tree Lao Ye that food launches; The cocoanut tree Lao Ye section of launching is bundled into bundle, has simulated Brontispa longissima living environment like this, impel the cocoanut tree Lao Ye of Brontispa longissima above larva in 2 ages and imago feeding expansion; And keep not dehydration of blade, kept relative moisture.
In order to reduce cost, adopt the cocoanut tree Lao Ye that launches raise the Brontispa longissima adult and 2 ages above larva, host material obtains easily; Can not cause too many influence to the cocoanut tree growth; And raise with the cocoanut tree Lao Ye that launches, accelerated the Brontispa longissima growth, shortened the breeding cycle of Brontispa longissima.
In order to reduce the lethality of Brontispa longissima, the cocoanut tree Lao Ye of expansion will keep fresh and can not occupy moisture.The cocoanut tree Lao Ye that launches can leave 4 ℃ of refrigerator-freezers in, and storage period reaches 10 days.
In order to prevent that raising population for a long time degenerates, and answers the wild Brontispa longissima population of regular replenishment.
For the production that intensifies, insect box of the present invention (200 mm * 140 mm * 75 mm), lid central authorities be provided with long for 110mm, widely be the rectangle hole of 40mm, last 200 purpose gauzes fasten with glue.Said foster worm frame is processed by universal steel angle, long 2 m, wide 0.6 m, high 1.8 m.Each brandreth divides four layers, the bottom is liftoff 0.2 m, and every interlamellar spacing 0.4 m is furnished with 1 40w fluorescent tube for every layer; Four legs stand on the pallet that is filled with water, and prevent the Brontispa longissima that the ant infringement is raised.
In order to prevent in the Brontispa longissima feeding process that the generation of popular disease is regularly sterilized to receptacle and raising apparatus.
This raising method is mainly used in produces each worm attitude of Brontispa longissima in batches.
The present invention has following beneficial effect: the present invention with the cocoanut tree Lao Ye that launches replace lobus cardiacus raise adult and 2 ages above larva, reduced cost, and practicality easy and simple to handle, can carry out continuously, balanced, breed each instar of Brontispa longissima in batches.The survival rate average out to 96% of average 95%, 2 instar larvae of survival rate of incubation rate average out to 92%, 1 instar larvae of Brontispa longissima ovum; The survival rate average out to 98% of 3 instar larvaes,, 4 instar larvae survival rate average out to 99%; 5 instar larvae survival rate average out to 99%, pupa eclosion rate average out to 99%.Feeding cost is only raised with the cocoanut tree lobus cardiacus and is reduced 2/3rds, and per generation breeding cycle shortens 8 days.
Embodiment
The a large amount of propagation methods of Brontispa longissima of the present invention may further comprise the steps:
(1) medicine is taked not spray in the field, and the cocoanut tree Lao Ye that no scab and insect infringement launch is placed on 4 ℃ of refrigerator-freezers, and is subsequent use.Open in the lid central authorities of sealed plastic crisper (200 mm * 140 mm * 75 mm) that (110mm * 40mm), seal with 200 purpose gauzes then processes insect box flatly.Insect box is placed on supports on the worm frame.Support the worm frame by process long 2 m, wide 0.6 m, high 1.8 m by universal steel angle.Each brandreth divides four layers, the bottom is liftoff 0.2 m, and every interlamellar spacing 0.4 m is furnished with 1 40w fluorescent tube for every layer.Foster worm is placed on temperature 24-27 ℃, and relative moisture 75% ± 5% is in the insectary of natural lighting.
(2) the cocoanut tree Lao Ye that launches is cut into the segment of 150mm, pricks 1 bundle with rubber band, put into insect box for per 50.Introduce 500 pairs of Brontispa longissimas then and just incubate adult, let it get food and lay eggs, per 3 days replacing primary vane.When changing blade, prick the blade bulk storage that had Brontispa longissima originally at the old blade of the cocoanut tree of changing.Brontispa longissima can be searched for new food source, climbs to the cocoanut tree leaf of replacing automatically.All climb to Deng Brontispa longissima (about 1 day) behind the blade of new replacing, original blade is taken out, accomplish the replacing of blade.Brontispa longissima can be laid eggs on the cocoanut tree leaf after 10 days, and 3-5 grain ovum is end to end usually, vertically arranges.Pick out the blade that has ovum, concentrate and put into another insect box, about 2000 every boxes of ovum.
(3) ovum is through beginning hatching after 4-5 days, during to keep the humidity of environment.The cocoanut tree Lao Ye that the newly hatched larvae aphagia launches can only get food cocoanut tree lobus cardiacus.So cocoanut tree lobus cardiacus before hatching that 10 left and right sides 150mm are long is sneaked into the blade that has ovum, newly hatched larvae can be climbed to automatically and get the food growth on the lobus cardiacus.To have newly hatched larvae cocoanut tree lobus cardiacus and take out, and concentrate and put into another insect box.About 3 days of 1 instar larvae phase, change the old blade of cocoanut tree that launches after 3 days.Get into the cocoanut tree Lao Ye of the desirable food expansion of Brontispa longissima of 2 instar larvaes.
(4) the 2 instar larvae phases of Brontispa longissima are about 4 days, and 3 instar larvae phases were about 5 days, and 4 instar larvae phases were about 6 days, and 5 instar larvae phases were about 7 days, and larval phase amounts to 25 days.The cocoanut tree Lao Ye that all desirable food of larva after 2 length of times launches, per 3 days replacing one lower blades.According to food ingestion, 2-3 instar larvae, every box can be raised 2000; 4-5 instar larvae, every box can be raised 1000.To keep ambient humidity and not dehydration of blade during this time, be also noted that the Brontispa longissima epidemic disease takes place.The insectary requires clean, and receptacle and raising apparatus are regularly sterilized.
After (5) 25 days, Brontispa longissima gets into pupa time, about 6 days of pupa time.Blade need be do not changed during this time, but ambient humidity will be kept.After pupa turns into adult, put into the cocoanut tree Lao Ye of expansion.Let it get food and lay eggs, get into the raising of generation down.
(6) Brontispa longissima is raised will to change every year and is replenished once open-air population and prevent that population from degenerating.
Imposing a condition down, contrasting the present invention and only raise Brontispa longissima effect such as following table with the cocoanut tree lobus cardiacus.
Claims (7)
1. a large amount of propagation methods of Brontispa longissima is characterized in that may further comprise the steps:
(1) raising of Brontispa longissima adult and bring out it and lay eggs
1. the cocoanut tree Lao Ye that launches is cut into the segment of 150mm, pricks 1 bundle with rubber band, put into insect box for per 50;
2. introduce 1000 Brontispa longissimas in each insect box and just incubate adult, the male and female ratio is 1 ︰ 1;
3. changed primary vane in per 3 days, when changing blade original blade bulk storage is tied up at new blade, the blade that the Brontispa longissima adult can climb to new replacing is automatically tied and is got food and grow;
4. Brontispa longissima begins to lay eggs after 10 days, and ovum originates in blade surface, and 3-5 grain ovum is an extended spread, and the blade that will have ovum when at every turn changing blade is chosen, and puts into another insect box with a collection of concentrating, and every box is put 2000 ovum;
(2) raising of ovum hatching and 1 instar larvae
1. ovum began hatching after 4-5 days, and cocoanut tree lobus cardiacus before hatching that 10 150mm are long is sneaked into the blade that has ovum, and newly hatched larvae can be climbed to automatically and get food on the lobus cardiacus;
2. 1 instar larvae phase was 3 days, and the cocoanut tree lobus cardiacus that will have 1 instar larvae after 3 days is put into the insect box that the cocoanut tree Lao Ye of expansion ties, and carries out the raising of larval phase;
(3) management of larval phase raising and pupa
1. 2-3 instar larvae, every box is raised 2000, changes 1 blade in per 3 days, if the dead worm of band mildew is arranged, should choose;
2. 4-5 instar larvae, every box is raised 1000, changes 1 blade in per 3 days, if the dead worm of band mildew is arranged, should choose;
3. 25 days larval phases, larvae pupation after 25 days, be 6 days pupa time, need not change blade, but will keep ambient humidity; When pupa turned into adult soon, the blade that more renews let it lay eggs, and got into next captive breeding from generation to generation.
2. a large amount of propagation methods of Brontispa longissima as claimed in claim 1 is characterized in that: each worm attitude raising condition is 24～27 ℃ of temperature, relative moisture 75% ± 10%, natural lighting.
3. a large amount of propagation methods of Brontispa longissima as claimed in claim 1, it is characterized in that: the cocoanut tree Lao Ye of expansion is cut into the segment of 150mm, and per 50 leaf sections band together with rubber band, are difficult for tension, are bundled into bundle and the leaf section does not come off and is as the criterion.
4. a large amount of propagation methods of Brontispa longissima as claimed in claim 1 is characterized in that: the cocoanut tree Lao Ye of the expansion of obtaining is stored in 4 ℃ of refrigerator-freezers.
5. a large amount of propagation methods of Brontispa longissima as claimed in claim 1; It is characterized in that: said insect box length is 200 mm, and wide is 140 mm, and height is 75 mm; Lid central authorities be provided with long for 110mm, widely be the rectangle hole of 40mm, rectangle hole is provided with 200 purpose gauzes.
6. a large amount of propagation methods of Brontispa longissima as claimed in claim 5 is characterized in that: said insect box places supports on the worm frame, supports the worm frame and is processed by universal steel angle; Long 2 m, wide 0.6 m, high 1.8 m; Support the worm frame and divide four layers, the bottom is liftoff 0.2 m, every interlamellar spacing 0.4 m; Be furnished with 1 40w fluorescent tube for every layer, four legs of supporting the worm frame stand on the pallet that is filled with water.
7. a large amount of propagation methods of Brontispa longissima as claimed in claim 1 is characterized in that: annual from Brontispa longissima adult of field acquisition, all change the original seed worm.
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Cited By (4)
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| CN107494611A (en) * | 2017-08-28 | 2017-12-22 | 海南梵思科技有限公司 | A kind of Brontispa longissima preventing and treating Pesticidal tablet formulations and preparation method thereof |
| WO2021133835A1 (en) * | 2019-12-23 | 2021-07-01 | Manna Foods Co. | Insect farm |
| CN114208784A (en) * | 2022-01-21 | 2022-03-22 | 中国热带农业科学院环境与植物保护研究所 | Method for alternately attracting adult brontispa longissima based on two wavelength light sources |
| CN114586747A (en) * | 2022-04-15 | 2022-06-07 | 中国热带农业科学院环境与植物保护研究所 | Method for improving adult stage and survival rate of Chouioia cunea Yang |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107494611A (en) * | 2017-08-28 | 2017-12-22 | 海南梵思科技有限公司 | A kind of Brontispa longissima preventing and treating Pesticidal tablet formulations and preparation method thereof |
| WO2021133835A1 (en) * | 2019-12-23 | 2021-07-01 | Manna Foods Co. | Insect farm |
| CN114208784A (en) * | 2022-01-21 | 2022-03-22 | 中国热带农业科学院环境与植物保护研究所 | Method for alternately attracting adult brontispa longissima based on two wavelength light sources |
| CN114208784B (en) * | 2022-01-21 | 2023-05-23 | 中国热带农业科学院环境与植物保护研究所 | Method for alternatively attracting coconut leaf beetle adults based on two wavelength light sources |
| CN114586747A (en) * | 2022-04-15 | 2022-06-07 | 中国热带农业科学院环境与植物保护研究所 | Method for improving adult stage and survival rate of Chouioia cunea Yang |
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Application publication date: 20121114 |