CN114208784A - Method for alternately attracting adult brontispa longissima based on two wavelength light sources - Google Patents
Method for alternately attracting adult brontispa longissima based on two wavelength light sources Download PDFInfo
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- CN114208784A CN114208784A CN202210072240.7A CN202210072240A CN114208784A CN 114208784 A CN114208784 A CN 114208784A CN 202210072240 A CN202210072240 A CN 202210072240A CN 114208784 A CN114208784 A CN 114208784A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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Abstract
The invention belongs to the field of biotechnology of agriculture and forestry, and relates to a method for alternately attracting adult coconut leaf beetles based on two wavelength light sources. The invention makes full use of the characteristic that the adult coconut leaf beetles in the mating period are easy to migrate at night and show phototropism dark night activities, lures the adult coconut leaf beetles based on the staggering of two wavelength light sources, has good insect luring effect, simple and easy operation implementation mode, can promote the monitoring standardization under the condition of long-term use, and has positive significance for deeply knowing the occurrence trend and prediction of the field population of the coconut leaf beetles and developing physical killing in a large range by making and implementing the luring and killing method.
Description
Technical Field
The invention belongs to the field of biotechnology of agriculture and forestry, relates to a method for attracting brontispa longissima, and particularly relates to a method for alternately attracting brontispa longissima adults by utilizing two wavelength light sources.
Background
The brontispa longissima belongs to the coleoptera, the siderobinidae and the subnataceae, is an important pest which harms palmitaceae plants such as coconuts, is extremely harmful in southern areas of China for nearly ten years, is an important disaster prevention and reduction object in departments of agriculture and forestry and the like in China, and is rampant repeatedly and seriously affecting local economic and ecological environments in southern areas of China. The adult and larva of the brontispa longissima are fed on the epidermal tissues of the heart leaves of hosts such as a coconut tree and the like which are not unfolded, long and narrow brown stripe spots with parallel leaf veins are formed on the leaves, and large brown necrotic stripe spots appear after the heart leaves are unfolded, so that the whole plant is dead in severe cases. Because the palmaceae plants are generally straight and tall and are not easy to climb, and the brontispa longissima is mostly hidden in the heart leaves of the plants, the concealment is strong, and the method causes remarkable difficulty in timely knowing the occurrence level of the pests and implementing effective monitoring and control.
The phototaxis habit of insects is utilized, and the light insect-attracting technology is adopted to monitor and control agricultural pests, so that the method is one of the important means for comprehensively treating the agricultural pests at present. In China, as early as 50 s in the 20 th century, the kerosene lamp, the methane lamp, the incandescent lamp and the like are used for monitoring and controlling farmland pests, and then products such as the black light lamp, the high-pressure mercury lamp, the frequency vibration type trap lamp and the like appear in succession, so that the system is widely used for controlling agricultural and forestry pests. Although obvious effect is achieved in pest control, the problems of wide pest luring range, great influence on non-target insects, beneficial insects, natural enemies and biodiversity and the like exist in lamplight pest luring, and the method still moves forward in continuous groping.
Disclosure of Invention
The invention aims to provide a method for alternately attracting adult coconut leaf beetles based on two wavelength light sources, which utilizes day and night activity rhythms of day photophobia and night photophobia of the adult coconut leaf beetles to alternately attract the adult coconut leaf beetles to move and gather towards a target light source through the two wavelength light sources so as to achieve the trapping effect on the adult coconut leaf beetles, is convenient for implementing population monitoring and physical control in palm plant forests such as coconuts and the like, and provides a scientific and technical basis for developing and applying a lamplight-operated trapping technology and appropriately developing physical control in local areas.
The technical scheme adopted by the invention is as follows:
a method for alternately attracting adult coconut leaf beetles based on two wavelength light sources comprises the following steps:
1. two different attracting light sources are arranged on the insect attracting device, and the insect attracting device is hung on an attracting point of the brontispa longissima adults. The attraction light source is a purple light source and a green light source respectively.
2. The controller is used for controlling the alternate light emitting process of the attracting light source, the purple light source is driven to emit purple light with the wavelength of 390-395nm, the green light source is driven to emit green light with the wavelength of 520-523nm, the light emitting time of the purple light source is 3-5 times that of the green light source, and the alternate light emitting process is repeated to attract the brontispa longissima imagoes.
The insect trapping device (trap) used in the application is a device which is used for trapping and killing pests and structurally meets the requirement that the adult broccolite enters but cannot escape outwards. The trapping point is suspended at the height of 0.5-10 m by adopting the suspension mode of the conventional trap, and the trapping effect can be achieved.
Further, a protective cover, such as a plastic transparent protective cover, for protecting the attracting light source without changing the light propagation wavelength is arranged on the insect attracting device.
Furthermore, host leaves for inhabiting the brontispa longissima imagoes can be placed in the trapping device, and can be coconut leaves, palm macrocarpon leaves and the like, and fresh leaves are replaced in due time during the trapping period.
Furthermore, chemical insect killers such as various insecticides or physical insect killing devices such as a high-pressure insect killing net and the like are placed in the insect trapping device, so that the prevention and control effects of trapping and killing insects are achieved.
Further, the attraction light source is an LED lamp.
Furthermore, the attracting light source is within at least 10 meters of radius, and the illuminance is 500-12000 lx, so that the attracting effect of the adult brontispa longissima is achieved.
Compared with the prior art, the invention has the following beneficial effects:
1. the invention lures the brontispa longissima based on the two wavelength light sources in a staggered way, can lure the adult brontispa longissima by using a 4 th generation artificial light source LED as a luminous light source, has the advantages of high luminous efficiency, low power consumption, long service life, strong safety, controllable spectral range and the like, and actively responds to the development mode of low-carbon emission and low energy consumption in agriculture and forestry plant protection; and simultaneously, the working implementation mode of the two wavelength light sources with different switching durations is utilized, so that the working life of the light emitting source is effectively prolonged and protected, and social resources are saved.
2. The invention makes full use of the characteristic that the adult coconut leaf beetles in the mating period are easy to migrate at night and show phototaxis dark night activities, and alternately lures the adult coconut leaf beetles through two specific narrow-wavelength light sources. The problems of reduced biodiversity and the like caused by the mistaken entry of beneficial insects, natural enemies and other insects due to the wide light attraction range are avoided to a certain extent;
3. the light sources of the invention, namely the purple light 390-. The implementation mode is simple and easy to operate, monitoring standardization can be promoted under the condition of long-term use, and the establishment and implementation of the trapping and killing method have positive significance for deeply knowing the occurrence trend and prediction of the brontispa longissima field population and carrying out physical killing in a large range.
Detailed Description
The following examples are given to further illustrate the embodiments of the present invention. The following examples are intended to illustrate the invention but are not intended to limit the scope of the invention. The experimental procedures, in which specific conditions are not noted in the following examples, are generally carried out under conventional conditions or conditions recommended by the manufacturers.
Attracting the experimental apparatus: a regular octagonal prism closed feeding box (ensuring the dark condition of the feeding box) with the bottom surface side length of 25cm and the side edge length of 25cm is formed by enclosing black KT foam boards, 8 side surfaces of the feeding box are respectively provided with a circular opening with the diameter of 5cm, and the feeding box is respectively connected with 8 cube attracting boxes (formed by enclosing black KT foam boards) with the side length of 25cm, so that the feeding box is communicated with the attracting boxes through the circular openings.
The first embodiment is as follows: attraction of light sources with different wavelengths on brontispa longissima adults under dark conditions
Selecting 75 heads of female and male pupae of Brontispa longissima to be eclosized, and placing in the coconut leaf blade tied by rubber band. Put into a breeding box, and simultaneously, LED light sources of red 620-625nm, yellow 585-590nm, green 520-523nm, cyan 500-505nm, blue 460-465nm, purple-390-395 nm and purple 365-370nm, and white light LED lamps (wavelength range 450-455nm) are respectively randomly placed in 8 trapping boxes. Adjusting the illuminance of each light source to be 500lx by using a tailes TES1339 illuminometer, placing a small amount of fresh coconut leaves into the luring box, electrifying every 6 pm to enable 8 light sources to emit light for 14 hours or 16 hours, simultaneously recording the number of the gynophore and the androphore in the luring box of each LED light source, and putting the adult insects back into the feeding box for continuously recording for 15 days. The number of adult broccoli induced by light sources of different wavelengths is recorded in tables 1 to 4.
TABLE 1 quantity of Brontispa longissima adult lured by LED light sources with different wavelengths at 14 hours per day
TABLE 2 quantity of Brontispa longissima imagoes induced by LED light sources with different wavelengths at 14 hours per day
TABLE 3 number of Brontispa longissima adult lured by LED light sources with different wavelengths of 16 hours per day
TABLE 4 number of Brontispa longissima adults induced by LED light sources with different wavelengths at 16 hours per day
As shown in Table 1, in 15 days of 14 hours per day, the total attractable adult Brontispa longissima is 81 and 43 in purple 390-395nm and green 520-523nm, and the number of the attractable insect mouths is higher than that of other 6 LED light sources with different wavelengths; as shown in Table 2, the attracting speed of the brontispa longissima female adult is 54 heads in purple at 390-395nm every day, the attracting speed of the attracting speed is also obviously higher than that of attracting insect mouths of other 7 LED light sources with different wavelengths, and although 28 heads of attracting female insects with green 520-523nm, 32 heads of attracting male adults with color being 585-590nm and 39 heads of attracting male adults with color being 450-455nm are lower than that of attracting insect mouths of other 4 LED light sources, the attracting insect mouths of the attracting male adults with color being higher than that of attracting insect mouths of the other 4 LED light sources. As shown in Table 3, 16 hours a day, the total attractable adult Brontispa longissima is 54 heads and 35 heads in 15 days of purple 390-395nm and green 520-523nm, and the number of attractable insect mouths is higher than that of other 6 LED light sources with different wavelengths; as shown in Table 4, 16 hours a day, the total number of the imagoes of the brontispa longissima which can be attracted in 15 days of purple 390-395nm and green 520-523nm is 33 and 23 respectively, and the number of the attracted insects is higher than that of the attracted insects of other 6 LED light sources with different wavelengths. The results show that the purple 390-395nm and green 520-523nm LED light sources have obvious attraction effect on the brontispa longissima female and male worms.
Example two: attraction of two different wavelength light sources to brontispa longissima imagoes alternately under dark condition
Selecting 75 heads of female and male pupae of Brontispa longissima to be eclosized, and placing in a breeding box. Meanwhile, 2 opposite luring boxes are randomly selected from 8 luring boxes, wherein one luring box (marked as A box) is used for placing a white light LED lamp (with the wavelength range of 450-. Light insect-attracting mode: the white light LED lamps in the box A are electrified and emit light within 8 hours; the purple 390-395nm LED light source and the green 520-523nm LED light source in the B box are alternately electrified and emit light, wherein the ratio of the light emitting time of the purple 390-395nm LED light source to the light emitting time of the green 520-523nm LED light source is 3:1, namely under the condition of 8-hour electrified LED light source, the purple 390-395nm LED light source is firstly electrified and emits light for 3 hours and then is closed, and then the green 520-523nm LED light source is electrified and emits light for 1 hour and then is closed, and the steps are repeated. Adjusting the illumination intensity of each light source to 500lx by using a Teishi TES1339 illuminometer, putting a small amount of fresh coconut leaves into the luring boxes, electrifying the light sources to work for 8 hours every afternoon, simultaneously recording the number of the gynophore and the androphore in the two luring boxes (A box and B box), and putting the adult insects back into the feeding box for continuously recording for 15 days. The number of attracted brontispa longissima adults is recorded in table 5.
TABLE 5 number of adult Brontispa longissima lured by two light sources at 8 hours per day
As shown in Table 5, the total number of attractable adult and adult coilia ectus in 15 days is 41 and 37 respectively under the environment of alternating duration of 3:1 for 8 hours per day of LED light sources with purple color of 390-395nm and green color of 520-523nm, and the results are higher than the number of adult and adult coilia ectus (13 and 17 respectively) attracted by a white light LED lamp, and show that the LED light sources with the combination of purple color of 390-395nm and green color of 520-523nm have a remarkable attracting effect on the male and female coilia ectus under the environment of alternating duration of 3: 1.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the technical principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.
Claims (6)
1. A method for alternately attracting adult coconut heart shells based on two wavelength light sources is characterized by comprising the following steps:
1) two different attracting light sources are arranged on the insect attracting device, and the insect attracting device is hung on an attracting point of the brontispa longissima adults; the attraction light source is a purple light source and a green light source respectively.
2) The controller is used for controlling the alternate light emitting process of the attracting light source, the purple light source is driven to emit purple light with the wavelength of 390-395nm, the green light source is driven to emit green light with the wavelength of 520-523nm, the light emitting time of the purple light source is 3-5 times that of the green light source, and the alternate light emitting process is repeated to attract the brontispa longissima imagoes.
2. The method for alternately attracting adult coconut leaf beetles based on two wavelength light sources as claimed in claim 1, wherein the method comprises the following steps: the insect attracting device is provided with a protective cover for protecting the attracting light source without changing the light transmission wavelength.
3. The method for alternately attracting adult coconut leaf beetles based on two wavelength light sources as claimed in claim 1, wherein the method comprises the following steps: host leaves used for inhabiting the brontispa longissima imagoes are placed in the insect trapping device.
4. The method for alternately attracting adult coconut leaf beetles based on two wavelength light sources as claimed in claim 1, wherein the method comprises the following steps: chemical insect-killing agents or physical insect-killing devices are placed in the insect-attracting device.
5. The method for alternately attracting adult coconut leaf beetles based on two wavelength light sources as claimed in claim 1, wherein the method comprises the following steps: the attraction light source is an LED lamp.
6. The method for alternately attracting adult coconut leaf beetles based on two wavelength light sources as claimed in claim 1, wherein the method comprises the following steps: the attraction light source is within at least 10 m of radius, and the illuminance is 500-12000 lx.
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