CN114208784B - Method for alternatively attracting coconut leaf beetle adults based on two wavelength light sources - Google Patents

Method for alternatively attracting coconut leaf beetle adults based on two wavelength light sources Download PDF

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CN114208784B
CN114208784B CN202210072240.7A CN202210072240A CN114208784B CN 114208784 B CN114208784 B CN 114208784B CN 202210072240 A CN202210072240 A CN 202210072240A CN 114208784 B CN114208784 B CN 114208784B
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attracting
coconut leaf
light sources
adult
light source
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CN114208784A (en
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金涛
林玉英
马光昌
杨升院
郝铮
钟浩
温海波
彭正强
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CATAS Environment and Plant Protection Institute
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/033Rearing or breeding invertebrates; New breeds of invertebrates
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

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  • Environmental Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
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Abstract

The invention belongs to the technical field of agriculture and forestry biology, and relates to a method for alternately attracting adult coconut leaf beetles based on two wavelength light sources. The invention fully utilizes the characteristics of easy migration of the adult coconut leaf beetles in mating period at night and the dark night activity characteristic of phototaxis, and the invention is based on the staggered two-wavelength light sources to attract the adult coconut leaf beetles, has good insect attracting effect, simple implementation mode and easy operation, can promote monitoring standardization under the condition of long-term use, and formulation and implementation of the attracting method, and has positive significance for deep understanding of the occurrence trend and prediction of the coconut leaf beetle field population and wide-range physical killing.

Description

Method for alternatively attracting coconut leaf beetle adults based on two wavelength light sources
Technical Field
The invention belongs to the technical field of agriculture and forestry biology, relates to a method for attracting coconut leaf beetles, and in particular relates to a method for attracting coconut leaf beetle adults alternately by using two wavelength light sources.
Background
The coconut leaf beetle belongs to the sub-family of the iron beetle of the family Coleoptera, is an important pest which damages palm plants such as coconuts, is extremely harmful in the southern area of China in the last decade, is an important disaster prevention and reduction object for departments such as agriculture and forestry of China, and the like, and the repeated rampant occurrence seriously affects the local economy and ecological environment of the south of China. The coconut leaf beetle adults and larvae eat the undeployed pericarp epidermis tissues of hosts such as coco, so that long and narrow brown streaks with parallel veins are formed on leaves, and the developed heart leaves are large brown necrotic streaks, so that the whole plant dies seriously. Because the palmaceae plants are generally straight and tall and are not easy to climb, and the coconut palm leaf beetles are hidden in the heart leaves of the plants, the concealment is strong, and significant difficulties are caused to timely know the occurrence level of the insects and effectively monitor and control the insects.
The phototactic habit of insects is utilized, and the light insect attracting technology is adopted to monitor and control agricultural pests, so that the method is one of the important means for comprehensively controlling the agricultural pests at present. In the early 50 th 20 th century, china began to use kerosene lamps, biogas lamps, incandescent lamps and other products for monitoring and controlling farmland pests, and then black-light lamps, high-pressure mercury lamps, frequency vibration type insect-trapping lamps and other products appeared successively, so that the method is widely used for controlling agriculture and forestry pests. Although obvious effects are achieved in pest control, the light insect attracting has the problems of wide insect attracting range, large influence on non-target insects, beneficial insects, natural enemies and biodiversity and the like, and the light insect attracting is still continuously developed.
Disclosure of Invention
The invention aims to provide a method for alternately attracting adult coconut leaf beetles based on two wavelength light sources, which utilizes the day-light-avoiding and night-light-tending circadian rhythm of the adult coconut leaf beetles to alternately attract the adult coconut leaf beetles by the two wavelength light sources to promote the adult coconut leaf beetles to move and gather towards a target light source, achieves the effect of attracting the adult coconut leaf beetles, is convenient for implementing population monitoring and physical control in palm plant forests such as coconuts and the like, provides a scientific and technological basis for developing light-operated attracting monitoring of the adult coconut leaf beetles in the future and developing application of light attracting technology and physical prevention and control according to local conditions.
The invention adopts the technical scheme that:
a method for alternatively attracting coconut leaf beetle imago based on two wavelength light sources comprises the following steps:
1. two different attracting light sources are arranged on the insect attracting device, and the insect attracting device is hung on an attracting point of the adult coconut leaf beetles. The attracting light sources are respectively a purple light source and a green light source.
2. The controller is used for controlling the alternate light-emitting process of the attracting light source, the first driving ultraviolet light source emits ultraviolet light with the wavelength of 390-395nm, the second driving green light source emits green light with the wavelength of 520-523nm, wherein the light-emitting time of the ultraviolet light source is 3-5 times that of the green light source, and the alternate light-emitting process is repeated to attract the coconut leaf beetle adults.
The insect attracting device (trap) used in the application is a conventionally used device for attracting and killing insects, and can structurally meet the requirements that the adult coconut leaf beetles enter but cannot escape outwards. The trapping point is hung at the height of 0.5-10 m by adopting a hanging mode of a conventional trapper, so that the trapping effect can be achieved.
Further, the insect attracting device is provided with a protective cover, such as a plastic transparent protective cover, for protecting the attracting light source but not changing the light transmission wavelength.
Further, host leaves for inhabiting coconut leaf beetle adults are placed in the insect attracting device, and the host leaves can be coconut leaves, king palm leaves and the like, and fresh leaves should be replaced in time during attracting.
Further, chemical pesticides such as various pesticides or physical pesticide devices such as high-voltage pesticide nets and the like are placed in the insect attracting device, so that the insect attracting and controlling effect is achieved.
Further, the attracting light source is an LED lamp.
Further, the light source for attracting is at least within a radius of 10 meters, the illuminance is 500-12000 lx, and the effect of attracting the adult coconut leaf beetles is achieved.
Compared with the prior art, the invention has the following beneficial effects:
1. the invention is based on the staggered attraction of two wavelength light sources, can use the 4 th generation artificial light source LED as a light source to attract the adult coconut leaf beetles, has the advantages of high light emitting efficiency, less power consumption, long service life, strong safety, controllable spectrum range and the like, and actively responds to the development mode of low carbon emission and low energy consumption in the protection of agriculture and forestry plants; meanwhile, the working implementation modes of the two wavelength light sources with different switching time lengths are utilized, so that the working life of the light source is effectively prolonged and protected, and social resources are saved.
2. The invention fully utilizes the characteristic that the adult coconut leaf beetles in mating period are easy to migrate at night and show phototaxis night activity, and alternatively attracts the adult coconut leaf beetles by two specific narrow-wavelength light sources. The problems of reduced biodiversity and the like caused by false entry of beneficial insects, natural enemies and other insects due to wide lamp attracting range are avoided to a certain extent;
3. the light sources, namely the ultraviolet light 390-395nm and the green light 520-523nm, can be customized in a market, the application power of the light sources is planned according to the use area and the frequency, the material cost is low, and the operation standardization is facilitated. The implementation mode of the method is simple and easy to operate, the monitoring standardization can be promoted under the condition of long-term use, and the trapping method is formulated and implemented, so that the method has positive significance for deep understanding of the tendency and prediction of the occurrence of the coconut leaf beetle field population and wide-range physical killing.
Detailed Description
The following describes the embodiments of the present invention in further detail with reference to examples. The following examples are illustrative of the invention and are not intended to limit the scope of the invention. The experimental methods, in which specific conditions are not noted in the following examples, are generally conducted under conventional conditions or under conditions recommended by the manufacturer.
Attraction experimental device: the black KT foam plates are used for enclosing a regular eight-prism closed raising box (ensuring the dark condition of the raising box) with the bottom side length of 25cm and the side edge length of 25cm, circular openings with the diameter of 5cm are respectively formed in 8 sides of the raising box, and 8 square attracting boxes with the side length of 25cm are respectively connected (enclosed by the black KT foam plates), so that the raising box is communicated with the attracting box through the circular openings.
Embodiment one: attraction of light sources with different wavelengths to coconut leaf beetles under dark condition
75 male and female coconut leaf beetles to be eclosion are selected and placed in coconut leaves tightly tied by rubber bands. Put into a feeding box, and simultaneously, LED light sources of red 620-625nm, yellow 585-590nm, green 520-523nm, cyan 500-505nm, blue 460-465nm, purple 390-395nm and purple 365-370nm, and white LED lamps (wavelength range 450-455 nm) are randomly placed in 8 attracting boxes, respectively. The illuminance of each light source was adjusted to 500lx using a light meter of the Qinshi TES1339, a small amount of fresh coconut leaves were placed in the attractant box, 8 light sources were made to work for 14 hours or 16 hours by energizing every day at 6 pm, the number of female and male coco longissima insects in the attractant box for each LED light source was recorded simultaneously, and adults were put back into the feeder box again, and continuous recording was performed for 15 days. The number of light sources with different wavelengths for attracting the adult coconut leaf beetles is recorded as shown in tables 1 to 4.
TABLE 1 number of adult Eimeria nectar attractants attracted by LED light sources of different wavelengths for 14 hours per day
Figure BDA0003482532920000041
TABLE 2 number of adult Cocois leaf beetles attracted by different wavelength LED light sources 14 hours per day
Figure BDA0003482532920000042
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Figure BDA0003482532920000051
TABLE 3 number of adult female coconut leaf beetles attracted by LED light sources with different wavelengths for 16 hours per day
Figure BDA0003482532920000052
TABLE 4 number of adult coconut leaf beetles attracted by LED light sources of different wavelengths for 16 hours per day
Figure BDA0003482532920000053
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Figure BDA0003482532920000061
As shown in Table 1, the total number of the entireties of the coconut leaf beetles which can be enticed is 81 heads and 43 heads in 15 days with the colors of purple 390-395nm and green 520-523nm for 14 hours per day, which is higher than the number of the entireties of other 6 different wavelength LED light sources; as shown in Table 2, the number of the attracting insect mouths of the female adult coconut leaf beetles with the purple color of 390-395nm for 14 hours per day is also obviously higher than that of the attracting insect mouths of other 7 LED light sources with different wavelengths, while the number of the attracting insect mouths of the female adult coconut leaf beetles with the green color of 520-523nm is 28, the number of the attracting insect mouths of the female adult coconut leaf beetles with the green color of 585-590nm is lower than that of the attracting insect mouths of the male adult coconut leaf beetles with the orange color of 32 and the number of the attracting insect mouths of the male adult coconut leaf beetles with the white color of 450-455nm is higher than that of the attracting insect mouths of other 4 LED light sources. Table 3 shows that the total number of the entireties of the coconut leaf beetles which can be enticed is 54 heads and 35 heads in 15 days with the purple color of 390-395nm and the green color of 520-523nm in 16 hours per day, which is higher than the number of the entireties of other 6 different wavelength LED light sources; table 4 shows that the total number of the attractable coconut leaf beetle male adults is 33 heads and 23 heads, which are higher than the number of the insect attracting mouths of other 6 different-wavelength LED light sources, in 15 days of 16-hour purple 390-395nm and green 520-523 nm. These results show that the purple LED light source with the wavelength of 390-395nm and the green LED light source with the wavelength of 520-523nm has remarkable attracting effect on male and female insects of brontispa longissima.
Embodiment two: under dark condition, two light sources with different wavelengths alternately attract adult coconut leaf beetles
75 male and female cocoons of the coconut leaf beetles to be eclosion are selected and placed in a feeding box. Meanwhile, 2 opposite attracting boxes are selected from 8 attracting boxes at random, wherein one attracting box (marked as a box A) is provided with a white light LED lamp (with the wavelength range of 450-455 nm), and the other attracting box (marked as a box B) is provided with a purple light source of 390-395nm and a green light source of 520-523 nm. Lamp light insect attracting mode: the white light LED lamps in the box A are electrified to emit light in 8 hours; the LED light sources of 390-395nm and the LED light sources of 520-523nm in the B box are electrified alternately to emit light, wherein the light emitting time ratio of the LED light sources of 390-395nm to the LED light sources of 520-523nm in the B box is 3:1, namely under the condition of 8 hours of electrified LED light sources, the LED light sources of 390-395nm are electrified to emit light for 3 hours and then are closed, and then the LED light sources of 520-523nm in the B box are electrified to emit light for 1 hour and then are closed, so that the operation is repeated. The light intensity of each light source working at 500lx was adjusted by using a light intensity meter of the Qinshi TES1339, a small amount of fresh coconut leaves were put into the attracting box, the light source was made to work for 8 hours by energizing every day at 8 pm, the numbers of female and male coco longissima insects in the two attracting boxes (A box and B box) were recorded simultaneously, and the adults were put back into the feeding box again, and the continuous recording was carried out for 15 days. The number record of the imago attracting coconut leaf beetles is shown in table 5.
TABLE 5 number of adult coconut leaf beetles attracted by two light sources 8 hours per day
Figure BDA0003482532920000071
As shown in Table 5, the total attractable female and male adults of the coconut leaf beetles are 41 heads and 37 heads respectively in the environment with the alternating time length of 3:1 in 8 hours per day of LED light sources of purple 390-395nm and green 520-523nm, which are higher than the female and male adults (13 heads and 17 heads respectively) of the coconut leaf beetles attracted by the white light LED lamp, the results show that the LED light sources combined with purple 390-395nm and green 520-523nm have remarkable attraction effect on the female and male worms of the coconut leaf beetles in the environment with the alternating time length of 3:1.
The foregoing is merely a preferred embodiment of the present invention, and it should be noted that it will be apparent to those skilled in the art that several modifications and variations can be made without departing from the technical principle of the present invention, and these modifications and variations should also be regarded as the scope of the invention.

Claims (3)

1. A method for alternatively attracting coconut leaf beetle adults based on two wavelength light sources is characterized by comprising the following steps:
1) Two different attracting light sources are arranged on the insect attracting device, and the insect attracting device is hung on an attracting point of the adult coconut leaf beetles; the attracting light sources are respectively a purple 390-395nm LED light source and a green 520-523nm LED light source; host leaves for inhabiting the adult coconut leaf beetles are placed in the insect attracting device;
2) The controller is used for controlling the alternate light-emitting process of the attracting light source, the ultraviolet light source is driven by the precursor to emit ultraviolet light with the wavelength of 390-395nm, the green light source is driven by the precursor to emit green light with the wavelength of 520-523nm, the light-emitting time of the night ultraviolet light source is 3-5 times that of the green light source, and the alternate light-emitting process is repeated to attract the adult coconut leaf beetles; the light source for attracting is within a radius of at least 10 meters, and the illuminance is 500-12000 lx.
2. A method of alternately attracting adult coconut leaf beetles based on two wavelength light sources as recited in claim 1 wherein: the insect attracting device is provided with a protective cover for protecting the attracting light source but not changing the light transmission wavelength.
3. A method of alternately attracting adult coconut leaf beetles based on two wavelength light sources as recited in claim 1 wherein: the chemical pesticide or the physical pesticide is put into the insect attracting device.
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