CN101731186B - Artificial rearing method of Grapholitha molesta Busck - Google Patents
Artificial rearing method of Grapholitha molesta Busck Download PDFInfo
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- CN101731186B CN101731186B CN2010101074396A CN201010107439A CN101731186B CN 101731186 B CN101731186 B CN 101731186B CN 2010101074396 A CN2010101074396 A CN 2010101074396A CN 201010107439 A CN201010107439 A CN 201010107439A CN 101731186 B CN101731186 B CN 101731186B
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Abstract
The invention discloses an artificial rearing method of Grapholitha molesta Busck. The method is implemented by inoculating Grapholitha molesta Busck eggs on apples, after larvae are incubated and eat into the apples for 5-6 days, stripping out the larvae to be inoculated into a finger-shaped tube filled with artificial feed until larvae cocoon, pupate and emerge, putting mixed emerged male and female imagoes into a beaker to propagate to form an experimental population under certain rearing conditions. The method is simple, convenient and is low in rearing cost for indoor successive rearing.
Description
Technical field
This research relates to the raising method of the raising method of a kind of fruit tree pest insect, particularly a kind of oriental fruit moth.
Background technology
Oriental fruit moth Grapholitha molesta Busck is a kind of global fruit tree pest insect, blazons Asia, Europe, America and Australia.China is not except that appearing in the newspapers in Tibet, and other each province (city, district) all has generation, takes place general with North China, Central China especially.The oriental fruit moth host plant comprises rosaceous multiple fruit tree, mainly with larva moth food pears, peach, the fruit of apple and the young sprout of peach.Generally serious in the harm of the orchard of fruit tree companion plantings such as peach, pears.In recent years, because the adjustment of the agricultural production industrial structure, the fruit growing kind increases, enlarged areas, many regional oriental fruit moth have the trend that increases the weight of year by year in China, and the wormed fruit rate in the serious theatre that partly is injured has a strong impact on the quality and yield of fruit up to more than 70%.
For effectively controlling causing harm of oriental fruit moth, expansion such as biology, ecology and chemical control technology further investigation to oriental fruit moth is a basic job, and particularly preventing and treating the screening of oriental fruit moth effective agent, the toxicologic study of medicament effect etc. must have raised growth to grow consistent examination worm.Given this, how to raise a large amount of examination of acquisition worms and just become one of important step of oriental fruit moth research by indoors artificial.Domesticly do not see oriental fruit moth artificial feeding Study on Technology report so far as yet, only Zhang Xingzheng (1980) has studied oriental fruit moth each generation from generation to generation and the relation at temperature and sunshine under field conditions (factors).Abroad as far back as 1956, Chaudhry just studied the developmental state of oriental fruit moth under constant temperature, wet surely condition, and the result shows that under the condition of relative moisture 70% along with the rising of temperature, the development duration of ovum, larva, pupa shortens gradually.1987, Yokoyama was 26.8 ℃ in temperature, under the condition of relative moisture 60% and photoperiod 16:8 (L:D), had obtained success the laboratory rearing oriental fruit moth.1989, Vetter report thought 25 ± 1 ℃ of temperature, artificial feeding oriental fruit moth in a large number under relative moisture 50 ± 5% and photoperiod 16:8 (L:D) condition.
The insect that oriental fruit moth distributes as a kind of world wide, the population of different regions has formed certain adaptitude to the local ecotope in long-term isolation evolutionary process.The applicant once adopted above-mentioned external used breeding technology to raise, and the result shows ovum and larval mortality height, and the adult fertility is very low, can not raise by the continuous multi-generation subculture.
Summary of the invention
At the problem and the needs of carrying out oriental fruit moth biology, ecological further investigation that lack enough standardization examination worms in oriental fruit moth control medicament screening and the pesticide toxicology research, the objective of the invention is to, a kind of oriental fruit moth indoors artificial raising method is provided.
In order to realize above-mentioned task, the present invention takes following technical solution:
The rearing method of a kind of oriental fruit moth is characterized in that, this method adopts apple and artificial feed to combine and raises oriental fruit moth, comprises the following steps:
A, at first dispose the oriental fruit moth artificial feed, sealing refrigeration in 4 ℃ of refrigerators, ready access upon use;
B, the oriental fruit moth ovum being connected on through the hollow place of the apple calyx of the ethanol disinfection of mass fraction 75%, is 26 ± 0.5 ℃ in temperature, relative moisture 70 ± 10%, and photoperiod 15:9 (L:D) h, intensity of illumination 4 is raised under the condition of 800lux;
C, eat into ovum hatching larva and to get food in the apple 5-6 days, before apple be about to rot, larva is stripped out from apple and inserts in the dactylethrae that about 3g artificial feed are housed, pack into 5 of larvas of every pipe, tampon seals, and continues to put into the climatic cabinate of above-mentioned temp. and humidity and illumination condition and raises;
D, larva after cocooing and pupating strip out pupa, put into insect box, and the built-in wet cotton balls of box is preserved moisture;
After E, pupa turn into adult, first emergence adult male and female are mixed, be one group with 20～30 and put into a 2000ml beaker, placing in the cup as the template of laying eggs and one is that the apple of 75% ethanol disinfection is laid eggs for it through mass fraction, and place dip in content be the cotton balls of 5% sucrose water as the adult source that supplements the nutrients, change template and apple every day.
The food that described larva hatches the initial stage is apple.
Described artificial feed prescription is: corn flour: 75g, soybean meal: 75g, dusty yeast: 30g, catsup: 198g, ascorbic acid: 3g, cholesterol: 0.1g, sorbic acid: 1g, methyl hydroxybenzoate: 2g, agar: 14g, water: 700g.
Apple is selected the apple of gathering after the maturation for use, and kind is a Fuji.
Artificial feeding of the present invention is simple and convenient, and feeding cost is low, can raise at indoor subculture, obtains the consistent oriental fruit moth worm source of enough growing.
Embodiment
In order clearly to understand the present invention, the present invention further is described in detail below in conjunction with embodiment.
Rearing method according to oriental fruit moth of the present invention specifically comprises the steps:
1, preparation oriental fruit moth artificial feed: take by weighing and put into pot behind corn flour 75g, soybean meal 75g, the dusty yeast 30g mixing and steam behind the 30min stand-by; Get agar 14g adding distil water 700ml and boil until agar and dissolve fully, add sorbic acid 1g, methyl hydroxybenzoate 2g, ascorbic acid 3g and cholesterol 0.1g when being cooled to 50 ℃, stir until dissolving; Adding is steamed the mixture of good corn flour, analysis for soybean powder and dusty yeast and is stirred, add catsup 198g at last, pour into the crisper behind the 0.2% clorox soaking disinfection 12h (in 18 * 12 * 6cm) after stirring rapidly, be cut into the fritter of 3 * 1 * 1cm after cooling, 4 ℃ of sealing refrigerations in the refrigerator, ready access upon use.
2, with the ethanol disinfection of apple with mass fraction 75%, to put into after drying in the crisper (diameter 18cm, height 10cm), every box is put 4, and the calyx low-lying area is up.The oriental fruit moth ovum is placed on the calyx low-lying area together with the paper of laying eggs locates or be placed directly in the crisper, each apple approximately connects 100 on ovum.Placing temperature then is 26 ± 0.5 ℃, relative moisture 70 ± 10%, and photoperiod 15:9 (L:D) h, intensity of illumination 4 is raised in the climatic cabinate of 800lux.
3, larva hatching is invaded apple and was got food 5-6 days, and apple strips out larva with tweezers or pocket knife before being about to rot from apple, insert in the dactylethrae (diameter 1.5cm, highly 10cm) that about 3g artificial feed are housed, and pack into 5 of larvas of every pipe, tampon seals.Continuing to put into the climatic cabinate of above-mentioned temp. and humidity and illumination condition raises.
4, after larva cocoons and pupates, pupa is stripped out, put into insect box (diameter 13cm, height 6cm), the built-in wet cotton balls of box is preserved moisture.
5, after pupa turns into adult, first emergence adult male and female are mixed, 20-30 puts into a 2000ml beaker for first group, place in the cup template (10 * 20cm) and sterilization after apple lay eggs for it, and place the cotton balls that dips in 5% sucrose water as the adult source that supplements the nutrients.Change template and apple every day.
6, the evaluation of feeding effect: the applicant uses method of the present invention in 20 generations of indoor continuous raising oriental fruit moth, measure the main life parameters of each worm attitude respectively in the 1st, 5,10,15,20 generations, the result shows that the life parameters difference between each generation is very little, population development neat (seeing attached list).Illustrate and adopt this artificial raising method can satisfy the requirement that the indoor subculture of oriental fruit moth is raised fully, grow the examination worm of neat and consistent to obtain raised growth.
Subordinate list oriental fruit moth indoors artificial is raised life parameters table from generation to generation
Annotate: data are mean value ± standard error in the table, represent through new multipole difference multiple ratio back difference not remarkable (P＞0.05) with same letter behind the line data.
Claims (3)
1. the rearing method of an oriental fruit moth is characterized in that, the method that this method adopts apple and artificial feed to combine is raised oriental fruit moth, comprises the following steps:
A, at first prepare the oriental fruit moth artificial feed, sealing refrigeration in 4 ℃ of refrigerators, ready access upon use;
B, the oriental fruit moth ovum being connected on through the hollow place of the apple calyx of the ethanol disinfection of mass fraction 75%, is 26 ± 0.5 ℃ in temperature, relative moisture 70 ± 10%, and photoperiod L: D=15: 9, intensity of illumination 4 is raised under the condition of 800lux;
C, eat into ovum hatching larva and to get food in the apple 5-6 days, before apple is about to rot, larva is stripped out from apple and inserts in the dactylethrae that about 3g oriental fruit moth artificial feed are housed, pack into 5 of larvas of every pipe, tampon seals, and continues to put into the climatic cabinate of above-mentioned temp. and humidity and illumination condition and raises;
D, larva after cocooing and pupating strip out pupa, put into insect box, and the built-in wet cotton balls of box is preserved moisture;
After E, pupa turn into adult, first emergence adult male and female are mixed, be one group with 20～30 and put into a 2000ml beaker, placing in the cup as the template of laying eggs and one is that the apple of 75% ethanol disinfection is laid eggs for it through mass fraction, and place dip in content be the cotton balls of 5% sucrose water as the adult source that supplements the nutrients, change template and apple every day.
2. the method for claim 1 is characterized in that, the food that described larva hatches the initial stage is apple.
3. the method for claim 1 is characterized in that, described artificial feed prescription is: corn flour: 75g, soybean meal: 75g, dusty yeast: 30g, catsup: 198g, ascorbic acid: 3g, cholesterol: 0.1g, sorbic acid: 1g, methyl hydroxybenzoate: 2g, agar: 14g, water: 700g.
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| CN2010101074396A CN101731186B (en) | 2010-02-08 | 2010-02-08 | Artificial rearing method of Grapholitha molesta Busck |
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| CN2010101074396A CN101731186B (en) | 2010-02-08 | 2010-02-08 | Artificial rearing method of Grapholitha molesta Busck |
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| CN101731186A CN101731186A (en) | 2010-06-16 |
| CN101731186B true CN101731186B (en) | 2011-08-31 |
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Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103503838B (en) * | 2013-10-15 | 2015-05-20 | 广东省农业科学院植物保护研究所 | Method for rearing litchi fruit borers |
| CN103892121B (en) * | 2014-03-27 | 2016-01-20 | 西北农林科技大学 | Man-made feeds of carpocapsa pononella and preparation method thereof |
| CN108849755A (en) * | 2018-06-10 | 2018-11-23 | 山西省农业科学院植物保护研究所 | Oriental fruit months larva store method |
| CN109984098B (en) * | 2019-05-15 | 2022-03-04 | 山西农业大学 | Propagation method for experimental population of oriental fruit moth |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1283173C (en) * | 2004-02-13 | 2006-11-08 | 中国科学院动物研究所 | Artificial feed for antlered larvae, its preparing method and use |
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1283173C (en) * | 2004-02-13 | 2006-11-08 | 中国科学院动物研究所 | Artificial feed for antlered larvae, its preparing method and use |
Non-Patent Citations (4)
| Title |
|---|
| D.A. Russel et al..The effect of diet, temperature and diapause on the number and identification of larval instars in the oriental fruit moth Grapholita molesta (Busck) (Lepidoptera:Tortricidae).《Agromomie》.1989,第9卷第919-926页. * |
| D.A.Russeletal..Theeffectofdiet temperature and diapause on the number and identification of larval instars in the oriental fruit moth Grapholita molesta (Busck) (Lepidoptera:Tortricidae).《Agromomie》.1989 |
| 侯月利等.利用苹果饲养桃小食心虫的技术.《陕西林业科技》.2004,(第2期),第24-26页. * |
| 厐艳从等.梨小食心虫的发生规律与防治.《河北林业科技》.2007,(第2期),第64页. * |
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