CN102283175A - Method for continuous rearing of campylomma lividicornis reuter by in-vitro plant material - Google Patents
Method for continuous rearing of campylomma lividicornis reuter by in-vitro plant material Download PDFInfo
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- CN102283175A CN102283175A CN2011101872846A CN201110187284A CN102283175A CN 102283175 A CN102283175 A CN 102283175A CN 2011101872846 A CN2011101872846 A CN 2011101872846A CN 201110187284 A CN201110187284 A CN 201110187284A CN 102283175 A CN102283175 A CN 102283175A
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Abstract
The invention provides a method for the continuous rearing of campylomma lividicornis reuter by an in-vitro plant material, which comprises the following steps: 1) placing an in-vitro plant material in a ventilated worm rearing box; 2) rearing adults and nymphs of campylomma lividicornis reuter in the worm rearing box, and supplying honey water in an adult period; and 3) transferring an egg-carrying plant material into another worm rearing box for continuous rearing. In the rearing method, the in-vitro plant material is available in farmers' markets or supermarkets all the year round and therefore can be obtained easily in unlimited number, so large-scale all-the-year-round rearing is facilitated. Meanwhile, the rearing method is simple in operation and the rearing effect is stable.
Description
Technical field
The present invention relates to insect and raise the field, particularly relate to a kind of method of utilizing the vegetable material subculture that exsomatizes to raise plant bug.
Background technology
Plant bug is the important pest of a class, kind is numerous, and China mainly contains green plant bug Apolygus lucorum (Meyer-D ü r.), black striped plant bug Adelphocoris suturalis (Jakovlev), adelphocoris taeniophorus Adelphocoris fasiaticollis (Reuter), alfalfa plant bug Adelphocoris lineolatus (Goeze) and tarnished plant bug Lygus pratensis (L.).The plant bug host plant is in extensive range, causing harm property is strong, and under adapt circumstance, population increases rapidly, breaks out and causes disaster.In recent years, along with the adjustment of China's agricultural structure and the establishing in large scale of transgenic cotton against pests, the plant bug population quantity sharply rises, and harm strengthens, and is serious catastrophe trend development.In China Huanghe valley and area, the Yangtze river basin, plant bug has risen to the primary insect of cotton, and the general time causes harm with a toll of 20-30%, and the serious time surpasses 50%.In addition, plant bug endangers also very serious on other crops such as jujube, grape, cherry, peach, apple and tea tree, and the production of above-mentioned multiple kinds of crops is constituted important threat.
At the new problem of the rampant harm of plant bug, be badly in need of carrying out researchs such as plant bug biological property, pests occurrence rule, prediction and integrated control technique.The standard that the various length of times and nutrition condition unanimity are provided for test is the basis of carrying out the every research of insect for the examination worm.China is someone's proposed to utilize live bodies such as cotton seedling, clover seedling, potato bud to exsomatize method that vegetable material raises plant bug in early days, but live plant is cultivated problems such as existing workload is big, weather conditions requirement height.Therefore, live body exsomatize vegetable material can only be used for plant bug short-term, raise on a small scale, breed with the anniversary and raise and be difficult to implement scaleization.Therefore, the subculture breeding technology of development plant bug is extremely urgent.
Summary of the invention
In order to address the above problem, the object of the present invention is to provide a kind of method of utilizing the vegetable material subculture that exsomatizes to raise plant bug.
A kind of method of utilizing the vegetable material subculture that exsomatizes to raise plant bug provided by the invention, it comprises the steps:
1) vegetable material that will exsomatize is put into ventilated insect box;
2) plant bug adult, nymph are put into insect box and raise, the adult stage is replenished hydromel;
3) will move into the stripped vegetable material of ovum and carry out subculture in another insect box and raise.
The vegetable material that exsomatizes in the described step 1) is removed remains of pesticide before feeding, wherein fresh kidney bean beanpod pre-treatment step is as follows: soak 10min with 0.5% liquor natrii hypochloritis, wash repeatedly with clear water subsequently, towel off dried when the beanpod free from extraneous odour; Adopt RP-420 type residual toxicity of pesticide to detect the residue of pesticide of beanpod again, it is stand-by to detect qualified back; The pre-treatment of described corn is for removing bract and filigree.
Also be included in the insect box inner bottom part in the described step 1) and add the shredded paper bar, to increase the special heterogeneity in the insect box.Improve special heterogeneity and can alleviate autotomy between phase mutual interference and kind between the plant bug individuality, help survival and the growth of plant bug.
Stripped vegetable material in the described step 1) is fresh kidney bean beanpod and/or fresh corn rod, and wherein fresh kidney bean beanpod preferably is applicable to adult and the nymph of raising green plant bug, black striped plant bug, adelphocoris taeniophorus, alfalfa plant bug and tarnished plant bug; Wherein fresh corn preferably is applicable to and raises green plant bug adult and nymph.
Stripped vegetable material in the described step 1) was changed 1 time in per 2 days.
The stripped vegetable material that changes the band ovum in the described step 3) dries processing.Preferably placed in the cool 1～2 day.Observed the preservation situation of 1 stripped vegetable material in per two days, excessive as humidity in the compartmentalized box for holding assorted fruits and candies, can at the bottom of box, spread one deck blotting paper with controlled humidity; As too dry in the compartmentalized box for holding assorted fruits and candies, can place 1～2 moistening rayon balls.The preservation overall principle of stripped vegetable material is " early stage, control was done, and the later stage preserves moisture ".
Described step 2) plant bug in is green plant bug, black striped plant bug, adelphocoris taeniophorus, alfalfa plant bug or tarnished plant bug.
Described step 2) insect box top covers gauze in, places a cotton balls that is soaked with hydromel on the gauze, supplements the nutrients for adult, and cotton balls replenishes 2 fresh hydromels every day, and the honeybee water concentration is preferably 5%; The gauze that insect box covers above need select for use 120 order gauzes to prevent that it from fleeing from for the low age nymph, and hold advanced age nymph or the insect box of adult can select common hospital gauze for use.
Described step 2) stocking density in, nymph are preferably 10～100/cubic decimeter, and more preferably 40～50/cubic decimeter, adult is preferably 5～60/cubic decimeter more preferably 25～35/cubic decimeter; The raising condition is 25～28 ℃ of temperature, relative moisture 60%～70%, and illumination L: D is 16: 8.The preferred rectangular plastic crisper of used insect box, size is 20cmx13cmx8cm.
The stripped vegetable material of the band ovum in the described step 3) be adult eclosion after 4 days until whole death during this period of time in the vegetable material that changes.
Subculture described in the described step 3) is raised, and the raising condition is preferably 25～28 ℃ of temperature, and relative moisture is preferably 60%～70%, and illumination L: D is 16: 8.Subculture is raised to be for many years and is raised continuously, and each is 25-30 days from generation to generation.
Up to now, utilize fresh kidney bean beanpod successfully to raise green plant bug generation more than 50, black striped plant bug, adelphocoris taeniophorus and alfalfa plant bug have been raised generation more than 30, and tarnished plant bug has been raised nearly 10 generations; Utilize fresh corn rod to raise green plant bug generation more than 10; In per generation,, raising scale was all more than 20000.
Stripped vegetable material is not only the food of plant bug adult, and is the carrier of laying eggs of adult.Green plant bug and tarnished plant bug are liked laying eggs in the wound of plant, need remove the kidney bean beanpod when therefore raising these two kinds of adults and be cut into two sections from intermediate lateral end to end or with corn, and the people is laid eggs for causing wound to be convenient to adult; Black striped plant bug, adelphocoris taeniophorus and alfalfa plant bug adult are directly placed complete stripped vegetable material and are got final product in raising.
Exsomatize in this raising method vegetable material in the market of farm produce or the supermarket median year sale is arranged, obtain easily, quantity is also unrestricted, is convenient to raise in the scale anniversary.Simultaneously, this raising method is simple to operate, and feeding effect is stable.
Embodiment
Following examples are used to illustrate the present invention, but are not used for limiting the scope of the invention.
Embodiment 1 plant bug feeding process
1, is ready to remove the fresh in vitro vegetable material of residue of pesticide.Fresh kidney bean beanpod pre-treatment step is as follows: soak 10min with 0.5% liquor natrii hypochloritis, wash repeatedly with clear water subsequently, towel off dried when the beanpod free from extraneous odour; Adopt RP-420 type residual toxicity of pesticide to detect the residue of pesticide of beanpod again, it is stand-by to detect qualified back; Corn only need be removed bract and filigree.
The nymph that 2, will just incubate is inserted the insect box that the fresh in vitro vegetable material is housed, and the insect box size is 20cm * 13cm * 8cm, and loam cake excises half with cutter, and with ventilated in the Bedpan, insect box top covers gauze.Every box worm amount is 100, and every kind of plant bug is established 12 repetitions (12 boxes just) on a kind of stripped vegetable material, and wherein 5 boxes are used for observed and recorded adult eclosion situation, counting nymph survival rate; Other 7 boxes are all normally raised, as the reserve worm source of adult test.Changed a fresh in vitro vegetable material in per 2 days, until whole emergence or death.The raising condition is 28 ℃ of temperature, relative moisture 60%, and illumination L: D is 16: 8.
3, behind the adult eclosion, shift out and insert new insect box subsequently, every box adult quantity is 60 (the male and female ratio is 1: 1).Every kind of plant bug is established 6 repetitions (6 boxes just) on a kind of stripped vegetable material.Day by day observe the survival condition of adult.Changed a fresh in vitro vegetable material, and replenished twice 5% hydromels every day in per 2 days.The stripped vegetable material that changes is checked the quantity of laying eggs under anatomical lens, and the incubation rate of test ovum.All dead until adult.
Result of the test shows, with green plant bug, black striped plant bug, adelphocoris taeniophorus, alfalfa plant bug and the tarnished plant bug of kidney bean beanpod raising and the green plant bug of raising with corn ear, nymphal development is neat, survival rate is high, the adult life-span is long, fertility is big, egg hatching rate height (table 1) is with the relevant parameter basically identical of reporting in the document of plant bug experimental population life table research in the past.Simultaneously, utilize fresh kidney bean beanpod successfully to raise green plant bug generation more than 50, black striped plant bug, adelphocoris taeniophorus and alfalfa plant bug have been raised generation more than 30, and tarnished plant bug has been raised nearly 10 generations; Utilize fresh corn rod to raise green plant bug generation more than 10.This explanation, two kinds of stripped vegetable materials provided by the invention and a kind of raising method can carry out the propagation in scale of plant bug and subculture is raised, and has solved the problem of plant bug " can not support, bad supporting ".
The feeding effect of plant bug on the different vegetable materials that exsomatize of table 1
Embodiment 2 plant bug stocking densities relatively
Comparative studies green plant bug, the feeding effect of black striped plant bug imago/nymph under different densities.Nymph is raised basic, normal, high 3 density is set, and is respectively 50,100,150 of every box nymphs, repeats 3 times, and the insect box size is 20cm * 13cm * 8cm.After access is just incubated nymph, observed 1 nymph survival condition in per 2 days, change fresh beanpod simultaneously, all sprout wings, the adult quantity that each processing of record is sprouted wings until all nymphs.Imago breeding is established 40,60,80 3 density of every box, repeats 3 times.The adult that has just sprouted wings is inserted insect box by density gradient, and the property ratio is 1: 1, observes once its adult survival condition, and changes fresh beanpod in per two days.Ovum amount above the beanpod that changes is checked under stereomicroscope, and carry out record, all dead until all female worms.Other raising method and condition are with embodiment 1.
Result of the test shows that under the high density, the nymph survival rate of black striped plant bug and green plant bug significantly is lower than low, middle density, after difference is all not remarkable between the two.Under the high density, the female average egg laying amount of the list of black striped plant bug adult is minimum, and significant difference between the egg laying amount under the low-density.The average egg laying amount difference of green plant bug all not significantly (table 2) under three kinds of density.Comprehensive above-mentioned result of the test, middle density help two kinds of plant bug subcultures and raise.
Table 2 stocking density is to the influence of black striped plant bug and green plant bug feeding effect
Embodiment 3 special heterogeneities are to raising the influence of plant bug
The comparative analysis special heterogeneity is to the influence of green plant bug and black striped plant bug feeding effect.(1cm * 20cm), with the hands gently rub to bending places the 10-15 root in the insect box bottom, is contrast with the insect box of not placing paper slip with scissors the A4 printing paper to be cut into microscler paper slip.Every box inserts just incubates 100 of nymphs or 60 of adults (adult property ratio is 1: 1), observes 1 its survival, the situation of laying eggs every 2 days, and changes fresh beanpod.Other raising method and condition are with embodiment 1.
Result of the test shows, adds in the insect box of paper slip, and the nymph survival rate of black striped plant bug and green plant bug, the single female egg laying amount of adult all are higher than and do not add the paper slip processing, and difference reaches significance level (table 3).This explanation is placed the paper slip of skim bending in the insect box bottom, can increase the special heterogeneity in the box, reduces the phase mutual interference between the plant bug individuality, helps nymph survival and adult and lays eggs.
Table 3 adds the influence of paper slip to black striped plant bug and green plant bug feeding effect
The above only is a preferred implementation of the present invention; should be pointed out that for those skilled in the art, under the prerequisite that does not break away from the technology of the present invention principle; can also make some improvements and modifications, these improvements and modifications also should be considered as protection scope of the present invention.
Claims (10)
1. one kind is utilized stripped vegetable material to raise the method for plant bug, and it comprises the steps:
1) vegetable material that will exsomatize is put into ventilated insect box;
2) plant bug adult, nymph are put into insect box and raise, the adult stage is replenished hydromel;
3) will move into the stripped vegetable material of ovum and carry out subculture in another insect box and raise.
2. method according to claim 1 is characterized in that, the stripped vegetable material described in the step 1) needs to remove remains of pesticide before feeding.
3. method according to claim 1 is characterized in that, also is included in the insect box inner bottom part in the step 1) and adds the shredded paper bar, to increase the special heterogeneity in the insect box.
4. method according to claim 1 is characterized in that, the stripped vegetable material of the band ovum described in the step 3) need dry processing.
5. according to any described method of claim 1～4, it is characterized in that the stripped vegetable material described in the step 1) is fresh kidney bean beanpod and/or fresh corn rod.
6. according to any described method of claim 1～4, it is characterized in that the stripped vegetable material described in the step 1) was changed 1 time in per 2 days.
7. according to any described method of claim 1～4, it is characterized in that step 2) in stocking density be 10～100/cubic decimeter of nymphs, 5～60/cubic decimeter of adults; The raising condition is 25～28 ℃ of temperature, relative moisture 60%～70%, and illumination L: D is 16: 8.
8. method according to claim 7 is characterized in that step 2) in stocking density be 40～50/cubic decimeter of nymphs, 25～35/cubic decimeter of adults.
9. according to any described method of claim 1～4, it is characterized in that step 2) described in plant bug be green plant bug, black striped plant bug, adelphocoris taeniophorus, alfalfa plant bug or tarnished plant bug.
10. according to any described method of claim 1～4, it is characterized in that step 2) described in the concentration of honeybee water be 5%.
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| CN104222010A (en) * | 2013-06-21 | 2014-12-24 | 王伯明 | Method for artificial mass production of predatory miridae |
| CN104642266A (en) * | 2015-01-14 | 2015-05-27 | 中国农业科学院植物保护研究所 | Method for raising green plant bug |
| CN105075992A (en) * | 2015-07-07 | 2015-11-25 | 华中农业大学 | Method for raising natural enemy insect southern minute pirate bug indoor in great deal and raising box |
| CN106879557A (en) * | 2017-02-17 | 2017-06-23 | 中国农业科学院棉花研究所 | A kind of method for raising black striped plant bug |
| CN106900657A (en) * | 2017-02-17 | 2017-06-30 | 中国农业科学院棉花研究所 | A kind of method that use live body host plant expands numerous black striped plant bug |
| CN107926863A (en) * | 2017-11-21 | 2018-04-20 | 浙江省农业科学院 | A kind of method of the light wing fleahopper of artificial propagation China |
| CN108496902A (en) * | 2018-06-06 | 2018-09-07 | 新疆农业科学院植物保护研究所 | A kind of tarnished plant bug breeding apparatus |
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104222010A (en) * | 2013-06-21 | 2014-12-24 | 王伯明 | Method for artificial mass production of predatory miridae |
| CN104222010B (en) * | 2013-06-21 | 2016-08-10 | 首伯农(北京)生物技术有限公司 | A kind of artificial a large amount of methods producing predatism fleahopper |
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| CN104642266A (en) * | 2015-01-14 | 2015-05-27 | 中国农业科学院植物保护研究所 | Method for raising green plant bug |
| CN105075992A (en) * | 2015-07-07 | 2015-11-25 | 华中农业大学 | Method for raising natural enemy insect southern minute pirate bug indoor in great deal and raising box |
| CN106879557A (en) * | 2017-02-17 | 2017-06-23 | 中国农业科学院棉花研究所 | A kind of method for raising black striped plant bug |
| CN106900657A (en) * | 2017-02-17 | 2017-06-30 | 中国农业科学院棉花研究所 | A kind of method that use live body host plant expands numerous black striped plant bug |
| CN107926863A (en) * | 2017-11-21 | 2018-04-20 | 浙江省农业科学院 | A kind of method of the light wing fleahopper of artificial propagation China |
| CN108496902A (en) * | 2018-06-06 | 2018-09-07 | 新疆农业科学院植物保护研究所 | A kind of tarnished plant bug breeding apparatus |
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Application publication date: 20111221 |