CN104642266A - Method for raising green plant bug - Google Patents
Method for raising green plant bug Download PDFInfo
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- CN104642266A CN104642266A CN201510019519.9A CN201510019519A CN104642266A CN 104642266 A CN104642266 A CN 104642266A CN 201510019519 A CN201510019519 A CN 201510019519A CN 104642266 A CN104642266 A CN 104642266A
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- green plant
- plant bug
- corn
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- kidney bean
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- 241000283615 Taylorilygus apicalis Species 0.000 title claims abstract description 66
- 238000000034 method Methods 0.000 title claims abstract description 30
- 240000008042 Zea mays Species 0.000 claims abstract description 40
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims abstract description 40
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims abstract description 40
- 235000005822 corn Nutrition 0.000 claims abstract description 40
- 241000819999 Nymphes Species 0.000 claims description 43
- 244000046052 Phaseolus vulgaris Species 0.000 claims description 40
- 239000005418 vegetable material Substances 0.000 claims description 25
- 238000000338 in vitro Methods 0.000 claims description 22
- 241000238631 Hexapoda Species 0.000 claims description 18
- 235000013305 food Nutrition 0.000 claims description 14
- 238000005286 illumination Methods 0.000 claims description 12
- 230000008859 change Effects 0.000 claims description 6
- 230000000384 rearing effect Effects 0.000 claims description 6
- 239000000575 pesticide Substances 0.000 claims description 4
- 238000002203 pretreatment Methods 0.000 claims description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 3
- 238000001514 detection method Methods 0.000 claims description 2
- 230000004083 survival effect Effects 0.000 abstract description 9
- 230000035558 fertility Effects 0.000 abstract description 2
- 244000013123 dwarf bean Species 0.000 abstract 2
- 230000002035 prolonged effect Effects 0.000 abstract 1
- 230000017448 oviposition Effects 0.000 description 7
- 238000012360 testing method Methods 0.000 description 7
- 241000196324 Embryophyta Species 0.000 description 5
- 238000011161 development Methods 0.000 description 5
- 239000000463 material Substances 0.000 description 4
- 229920000742 Cotton Polymers 0.000 description 3
- 244000299507 Gossypium hirsutum Species 0.000 description 3
- 230000037396 body weight Effects 0.000 description 3
- 238000009395 breeding Methods 0.000 description 3
- 230000001488 breeding effect Effects 0.000 description 3
- 230000006378 damage Effects 0.000 description 3
- 239000004677 Nylon Substances 0.000 description 2
- 241000607479 Yersinia pestis Species 0.000 description 2
- 238000005452 bending Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000003203 everyday effect Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 229920001778 nylon Polymers 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 244000144730 Amygdalus persica Species 0.000 description 1
- 241001048568 Apolygus lucorum Species 0.000 description 1
- 241000167854 Bourreria succulenta Species 0.000 description 1
- 235000009024 Ceanothus sanguineus Nutrition 0.000 description 1
- 102000002322 Egg Proteins Human genes 0.000 description 1
- 108010000912 Egg Proteins Proteins 0.000 description 1
- 241000258937 Hemiptera Species 0.000 description 1
- 240000003553 Leptospermum scoparium Species 0.000 description 1
- 235000015459 Lycium barbarum Nutrition 0.000 description 1
- 235000011430 Malus pumila Nutrition 0.000 description 1
- 244000070406 Malus silvestris Species 0.000 description 1
- 235000015103 Malus silvestris Nutrition 0.000 description 1
- 235000006040 Prunus persica var persica Nutrition 0.000 description 1
- 244000061456 Solanum tuberosum Species 0.000 description 1
- 235000002595 Solanum tuberosum Nutrition 0.000 description 1
- 241000219793 Trifolium Species 0.000 description 1
- 241001464837 Viridiplantae Species 0.000 description 1
- 235000009754 Vitis X bourquina Nutrition 0.000 description 1
- 235000012333 Vitis X labruscana Nutrition 0.000 description 1
- 240000006365 Vitis vinifera Species 0.000 description 1
- 235000014787 Vitis vinifera Nutrition 0.000 description 1
- 244000126002 Ziziphus vulgaris Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 235000019693 cherries Nutrition 0.000 description 1
- -1 dult Species 0.000 description 1
- 235000013601 eggs Nutrition 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 210000004681 ovum Anatomy 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 230000009261 transgenic effect Effects 0.000 description 1
- 230000007704 transition Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Cultivation Of Plants (AREA)
- Cultivation Receptacles Or Flower-Pots, Or Pots For Seedlings (AREA)
Abstract
The invention provides a method for raising a green plant bug. The method comprises the steps of raising a newly-hatched green plant bug instar larvae by adopting corn as feed, and then feeding the green plant bug by utilizing green bean pods after the instar larvae is feathered. The green plant bug instar larvae is fed by the corn and then the green plant bug adult is fed by utilizing the green bean pods, so that the survival rate of the green plant bug instar larvae is high, the developmental duration is shortened, the weight is increased, the service life of the green plant bug adult can be prolonged, and the fertility of the female green plant bug is improved.
Description
Technical field
The present invention relates to insect and raise field, specifically, relate to a kind of method of raising green plant bug.
Background technology
Green plant bug Apolygus lucorum (Meyer-D ü r) host range is extensive, nearly 288 kinds.Under adapt circumstance, population increases rapidly, break out and cause disaster.In recent years, along with the adjustment of China's agricultural structure and the establishing in large scale of transgenic cotton against pests, green plant bug population quantity sharply rises, and harm strengthens, and in serious catastrophe trend development.In China Huanghe valley and area, the Yangtze river basin, green plant bug has risen to the primary insect of cotton, and general time damage and yield loss reaches 20-30%, and the serious time is more than 50%.In addition, green plant bug endangers also very serious on other crops such as jujube, grape, cherry, peach, apple and tea tree, forms important threat to the production of above-mentioned multiple kinds of crops.
For the new problem of green plant bug Rampant damage, be badly in need of carrying out the researchs such as green plant bug biological property, pests occurrence rule, prediction and integrated control technique.Wherein for test provides the various length of time and the consistent standard of nutrition condition for examination worm, it is the basis of carrying out the every research of insect biology.Someone proposes the method that green plant bug raised by live plant materials such as utilizing cotton seedling, clover seedling, potato bud in early days in China, but live plant cultivates problems such as there is workload is large, weather conditions requirement height.Therefore, live plant material can only be used for green plant bug short-term, small-scale raising, and is difficult to implement scale and breeds and raise with the anniversary.Therefore, the anniversary breeding technology of the in vitro vegetable material of development green plant bug is needed badly.
Summary of the invention
In order to solve problems of the prior art, the object of this invention is to provide a kind of method of raising green plant bug.
Technical scheme of the present invention is as follows:
Raise a method for green plant bug, described method is be that fed food is raised with corn by the green plant bug nymph just incubated, until nymph sprout wings after with kidney bean beanpod for fed food is raised.
Further, described corn and kidney bean beanpod are fresh in vitro vegetable material.
Further, it is as follows that described kidney bean beanpod carries out pre-treatment step before feeding: after fresh kidney bean beanpod is soaked 10min with 0.5% liquor natrii hypochloritis, clear water rinses repeatedly, until kidney bean beanpod free from extraneous odour, dry rear detection residue of pesticide, qualified rear stand-by; Described corn removes bract and filigree before feeding.
As preferably, described fed food is changed 1 time for every 2 days.
Further, described green plant bug is raised in insectary, and stocking density is nymph 10-100 head/cubic decimeter, adult 5-60 head/cubic decimeter; Rearing conditions is temperature 25-28 DEG C, relative moisture 60%-70%, and periodicity of illumination L:D is 16:8.
As preferably, rearing conditions is temperature 28 DEG C, relative moisture 60%.
Further, said method comprising the steps of:
1) be equipped with in the insect box of fresh in vitro vegetable material corn by the green plant bug nymph just incubated access, upper cover excision half, with ventilated in Bedpan, insect box top covers gauze; The condition of raising insectary is temperature 28 DEG C, relative moisture 60%, and illumination L:D is 16:8h; Within every 2 days, change a fresh in vitro vegetable material, until all sprout wings;
2) the green plant bug nymph corn raised shifts out immediately and accesses the new insect box that fresh in vitro vegetable material kidney bean beanpod is housed, continuing at 28 DEG C, relative moisture 60% after sprouting wings, periodicity of illumination L:D be 16:8 insectary in raising;
Wherein, stocking density is nymph 10-100 head/cubic decimeter, adult 5-60 head/cubic decimeter.
As preferably, described fresh in vitro vegetable material corn is cut into 2cm thickness sheet.
As preferably, described fresh in vitro vegetable material kidney bean beanpod two ends are whittled into inclined-plane.
As preferably, the specification of described insect box is 20cm × 13cm × 8cm.
Beneficial effect of the present invention is:
The invention provides a kind of method utilizing in vitro vegetable material to combine raising green plant bug.The invention provides the grade of fit data of independent corn or kidney bean raising and two kinds of lower green plant bugs of food transitions raising under difficult environmental conditions, find to use corn to feed green plant bug nymph, then kidney bean beanpod is used to feed green plant bug adult, make that green plant bug nymph survival rate is high, development duration shortens, body weight increases, and be conducive to extending the life-span of green plant bug adult and improving the fertility of female worm.
By providing different vegetable material as food to respectively green plant bug nymph, adult in the present invention, develop a kind of method combinationally using vegetable material raising green plant bug, feeding efficiency significantly improves, utilizes separately the method for kidney bean beanpod continuously rearing green plant bug nymph and adult to there is essential difference simultaneously with prior art.
The method of the invention is simple to operate, and vegetable material corn, kidney bean are sold in the market of farm produce or supermarket median year, and easily obtain, quantity is also unrestricted, is convenient to raise in the scale anniversary.
Embodiment
Following examples for illustration of the present invention, but are not used for limiting the scope of the invention.
The preparation of the in vitro vegetable material of embodiment 1
Fresh kidney bean beanpod pre-treatment step is as follows: soak 10min with 0.5% liquor natrii hypochloritis, repeatedly rinse subsequently with clear water, until towel off dry during beanpod free from extraneous odour; Adopt RP-420 type residual toxicity of pesticide to detect the residue of pesticide of beanpod again, detect qualified rear stand-by.
Corn removes bract and filigree.
The raising of embodiment 2 green plant bug
1, the green plant bug nymph just incubated access is equipped with in the insect box (20cm × 13cm × 8cm) of fresh in vitro vegetable material corn (being cut into 2cm thickness sheet), upper cover excision half, with ventilated in Bedpan, insect box top covers gauze; The condition of raising insectary is temperature 28 DEG C, relative moisture 60%, and illumination L:D is 16:8h; Within every 2 days, change a fresh in vitro vegetable material, until all sprout wings;
2, after green plant bug nymph corn raised sprouts wings, shift out immediately and access the new insect box (20cm × 13cm × 8cm) that fresh in vitro vegetable material kidney bean beanpod (two ends are whittled into inclined-plane) is housed, continue at 28 DEG C, relative moisture 60%, periodicity of illumination L:D be 16:8 insectary in raise.
The individual feeding of embodiment 3 green plant bug
Proceed in the test tube of high 5cm, diameter 1.5cm by the green plant bug nymph single head of just incubating, the kidney bean beanpod placing an iblet or one section of 3cm length in pipe takes food for nymph, and the bending paper slip of placement one wide 1cm, long 5cm is movable for nymph simultaneously.After connecing worm, the mouth of pipe 80 object nylon wires are coverd with, then fix the mouth of pipe with rubber band, to prevent nymph escape.Then be placed on 25 DEG C, relative moisture 60%, illumination L:D raises in the incubator of 16:8h.Day by day check green plant bug nymph survival condition and the length of time situation of change.Turn into after adult until nymph, determine sex, weigh.Often kind of food is raised green plant bug nymph 4 groups, often organize 30.
Be used in the green plant bug adult that nymph that corn is raised just sprouted wings to match on corn and kidney bean respectively; single to each test tube of access; place an iblet or the long beanpod of one section of 3cm in test tube for imago feeding with lay eggs, provide the bending paper slip of a wide 1cm, long 5cm movable for it simultaneously.After access adult, the mouth of pipe 80 object nylon wires are coverd with, then fix the mouth of pipe with rubber band, escape to prevent it.Then be placed on 25 DEG C, relative moisture 60%, illumination L:D raises in the incubator of 16:8h.Day by day the survival condition of adult on corn, kidney bean and egg laying amount (corn changed every day, kidney bean count ovum amount under the microscope) is observed.In test tube till female worm death.Whole test terminates when the individual all death of all adults.Often kind of food is raised adult 4 groups, often group 20 is right.
Similarly, be used in the green plant bug adult that nymph that kidney bean is raised just sprouted wings to match on corn and kidney bean respectively.Method is the same.Day by day the survival condition of adult on corn, kidney bean and egg laying amount is observed.Often kind of food is raised adult 4 groups, often group 20 is right.
Result shows, with kidney bean is raised compared with green plant bug nymph, the green plant bug nymph survival rate that corn is raised is high, development duration shortens and after sprouting wings adult body weight increase, but the nymph of 2 plant material of feeding turn into adult after property ratio between not there are differences.When being transformed into the prolongation that green plant bug on kidney bean is female, the life-span of male imago raises on corn than continuation after the nymph emergence that corn is raised.When being transformed into the egg laying amount of the female worm of green plant bug on corn than the remarkable increase continuing to raise on kidney bean after the nymph emergence that kidney bean is raised.Result is as shown in table 1.
Table 1 green plant bug individual feeding result
Embodiment 4 green plant bug group breeding
Fresh in vitro vegetable material corn is equipped with in the green plant bug nymph just incubated access and (is cut into 2cm thickness sheet, 4/box) and kidney bean (two ends are whittled into inclined-plane, 4/box) insect box (20cm × 13cm × 8cm) in, upper cover cutter excises half, with ventilated in Bedpan, insect box top covers gauze.The condition of raising insectary is temperature 28 DEG C, and relative moisture 60%, illumination L:D is 16:8h.Every box worm amount incubates nymph at the beginning of 100, often kind of food is raised green plant bug nymph 12 box, and wherein 4 boxes are used for observed and recorded nymph and grow situation, turn into after adult, determine sex, weigh until nymph, and record and raise Start Date and emergence date.Other 8 boxes are all normally raised, as the standby worm source of adult test.Within every 2 days, change a fresh in vitro vegetable material, until all sprout wings or death.
After the green plant bug nymph that corn is raised sprouts wings, shift out immediately and access new insect box, every box adult quantity is 60 (sex ration is 1:1).4 boxes raised respectively by corn (being cut into 2cm thickness sheet, 4/box) and kidney bean (two ends are whittled into inclined-plane, 4/box).Continue at 28 DEG C, relative moisture 60%, illumination L:D be 16:8h insectary in raising.Day by day the survival condition (chosen by the green plant bug of death every day, and record date of death and male and female) of adult is observed.Within every 2 days, change a fresh in vitro vegetable material, the corn changed, kidney bean check Colony oviposition quantity under anatomical lens, until adult is all dead.
Similarly, the green plant bug nymph that kidney bean is raised shifts out immediately and accesses new insect box, matching respectively on corn and kidney bean after sprouting wings.Method is the same.Day by day the survival condition of adult on corn, kidney bean and Colony oviposition amount is observed.Often kind of food is raised adult 4 groups, often group 30 is right.
Result shows, in insectary, the green plant bug nymph that corn is raised higher than the survival rate that kidney bean is raised and after sprouting wings adult body weight increase, but not there are differences between the development duration of the nymph of 2 plant material of feeding and the property ratio after turning into adult.Be transformed into that green plant bug on kidney bean is female after the nymph raised when corn sprouts wings, the life-span of male imago and female worm egg laying amount be all than the remarkable increase continuing to raise on corn.And the nymph that kidney bean is raised is transformed into the egg laying amount of only female worm on corn than the increase continuing to raise on kidney bean after sprouting wings.Result is as shown in table 2.
Table 2 green plant bug group breeding result
This illustrates, the method that green plant bug is raised in the conversion of utilization provided by the invention in vitro vegetable material can carry out propagation in scale and the continuously rearing of green plant bug, solves the problem of green plant bug " can not support, bad support ", ensure that the year-round supply of green plant bug.
Although above the present invention is described in detail with a general description of the specific embodiments, on basis of the present invention, can make some modifications or improvements it, this will be apparent to those skilled in the art.Therefore, these modifications or improvements without departing from theon the basis of the spirit of the present invention, all belong to the scope of protection of present invention.
Claims (10)
1. raise a method for green plant bug, it is characterized in that, described method is be that fed food is raised with corn by the green plant bug nymph just incubated, until nymph sprout wings after with kidney bean beanpod for fed food is raised.
2. method according to claim 1, is characterized in that, described corn and kidney bean beanpod are fresh in vitro vegetable material.
3. method according to claim 2, it is characterized in that, it is as follows that described kidney bean beanpod carries out pre-treatment step before feeding: after fresh kidney bean beanpod is soaked 10min with 0.5% liquor natrii hypochloritis, clear water rinses repeatedly, until beanpod free from extraneous odour, dry rear detection residue of pesticide, qualified rear stand-by; Described corn removes bract and filigree before feeding.
4. the method according to any one of claim 1-3, is characterized in that, described fed food is changed 1 time for every 2 days.
5. method according to claim 4, is characterized in that, described green plant bug is raised in insectary, and stocking density is nymph 10-100 head/cubic decimeter, adult 5-60 head/cubic decimeter; Rearing conditions is temperature 25-28 DEG C, relative moisture 60%-70%, and periodicity of illumination L:D is 16:8.
6. method according to claim 5, is characterized in that, rearing conditions is temperature 28 DEG C, relative moisture 60%.
7. method according to claim 1, is characterized in that, said method comprising the steps of:
1) be equipped with in the insect box of fresh in vitro vegetable material corn by the green plant bug nymph just incubated access, upper cover excision half, with ventilated in Bedpan, insect box top covers gauze; The condition of raising insectary is temperature 28 DEG C, relative moisture 60%, and periodicity of illumination L:D is 16:8h; Within every 2 days, change a fresh in vitro vegetable material, until all sprout wings;
2) the green plant bug nymph corn raised shifts out immediately and accesses the new insect box that fresh in vitro vegetable material kidney bean beanpod is housed, continuing at 28 DEG C, relative moisture 60% after sprouting wings, illumination L:D be 16:8h insectary in raising;
Wherein, stocking density is nymph 10-100 head/cubic decimeter, adult 5-60 head/cubic decimeter.
8. method according to claim 7, is characterized in that, described fresh in vitro vegetable material corn is cut into 2cm thickness sheet.
9. method according to claim 7, is characterized in that, described fresh in vitro vegetable material kidney bean beanpod two ends are whittled into inclined-plane.
10. method according to claim 7, is characterized in that, the specification of described insect box is 20cm × 13cm × 8cm.
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Cited By (7)
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CN105379682A (en) * | 2015-10-19 | 2016-03-09 | 贵州大学 | Coridius chinensis large-scale artificial breeding technique |
CN107926863A (en) * | 2017-11-21 | 2018-04-20 | 浙江省农业科学院 | A kind of method of the light wing fleahopper of artificial propagation China |
CN108496900A (en) * | 2018-04-24 | 2018-09-07 | 云南农业大学 | Prevent the method for breeding and application method of Moringa Nao snout moth's larva Predacious functions |
CN108967364A (en) * | 2018-07-28 | 2018-12-11 | 云南省普洱茶树良种场 | One grows tea the method for breeding of angle fleahopper |
CN112314534A (en) * | 2020-11-26 | 2021-02-05 | 河北省农林科学院植物保护研究所 | Method for artificially feeding cerasus punctatus in whole growth period |
CN113349162A (en) * | 2021-07-09 | 2021-09-07 | 福建省农业科学院植物保护研究所 | Single-head indoor subculture method for Carica papaya mealybug |
CN114982721A (en) * | 2022-05-13 | 2022-09-02 | 河南农业大学 | Rapid propagation method of lygus lucorum |
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Cited By (10)
Publication number | Priority date | Publication date | Assignee | Title |
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CN105379682A (en) * | 2015-10-19 | 2016-03-09 | 贵州大学 | Coridius chinensis large-scale artificial breeding technique |
CN105379682B (en) * | 2015-10-19 | 2018-04-20 | 贵州大学 | The extensive artificial fecundation method of aspongopus |
CN107926863A (en) * | 2017-11-21 | 2018-04-20 | 浙江省农业科学院 | A kind of method of the light wing fleahopper of artificial propagation China |
CN108496900A (en) * | 2018-04-24 | 2018-09-07 | 云南农业大学 | Prevent the method for breeding and application method of Moringa Nao snout moth's larva Predacious functions |
CN108496900B (en) * | 2018-04-24 | 2020-11-24 | 云南农业大学 | Breeding method and using method for preventing and treating lygus tridentatus for moringa oleifera naotus |
CN108967364A (en) * | 2018-07-28 | 2018-12-11 | 云南省普洱茶树良种场 | One grows tea the method for breeding of angle fleahopper |
CN112314534A (en) * | 2020-11-26 | 2021-02-05 | 河北省农林科学院植物保护研究所 | Method for artificially feeding cerasus punctatus in whole growth period |
CN113349162A (en) * | 2021-07-09 | 2021-09-07 | 福建省农业科学院植物保护研究所 | Single-head indoor subculture method for Carica papaya mealybug |
CN113349162B (en) * | 2021-07-09 | 2023-09-26 | 福建省农业科学院植物保护研究所 | Single-head indoor subculture method for papaya gecko |
CN114982721A (en) * | 2022-05-13 | 2022-09-02 | 河南农业大学 | Rapid propagation method of lygus lucorum |
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