CN107926863A - A kind of method of the light wing fleahopper of artificial propagation China - Google Patents

A kind of method of the light wing fleahopper of artificial propagation China Download PDF

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Publication number
CN107926863A
CN107926863A CN201711165330.6A CN201711165330A CN107926863A CN 107926863 A CN107926863 A CN 107926863A CN 201711165330 A CN201711165330 A CN 201711165330A CN 107926863 A CN107926863 A CN 107926863A
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China
Prior art keywords
fleahopper
china
light wing
rice
wing
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CN201711165330.6A
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田俊策
郑许松
谢宇凯
吕仲贤
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Zhejiang Academy of Agricultural Sciences
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Zhejiang Academy of Agricultural Sciences
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Priority to CN201711165330.6A priority Critical patent/CN107926863A/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/033Rearing or breeding invertebrates; New breeds of invertebrates

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Zoology (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Pretreatment Of Seeds And Plants (AREA)

Abstract

The invention discloses a kind of method of the light wing fleahopper of artificial propagation China, comprise the following steps:In 26 28 DEG C of temperature, under conditions of humidity 65% 75%, using rice moth egg as food, the raising of the light wing fleahopper of China is carried out as the medium for providing moisture and oviposition with kidney bean, rice or degreasing cotton.The light wing fleahopper of China can give birth to substantial amounts of ovum on kidney bean, rice or degreasing cotton, and the kidney bean with ovum, rice or degreasing cotton are moved to new culture dish is raised by the method for breeding of the light wing fleahopper nymph of China.The light wing fleahopper nymph of China is raised 8 14 days, is transferred to after emerging adult in new culture dish, is raised by the method for breeding of the light wing fleahopper adult of China.The adult insect density of the light wing fleahopper of China>50, nymph insect density>When 50, ware is divided to raise.The present invention is easy to operate, cost is low, without season limit, is suitable for applying during industry is promoted.

Description

A kind of method of the light wing fleahopper of artificial propagation China
Technical field
The invention belongs to biological technical field, specifically, is related to a kind of method of the light wing fleahopper of artificial propagation China.
Background technology
Planthopper is the upper most important pest of rice in China production, and the means of prevention of mainstream is still chemical prevention at present. It is natural since chemical prevention causes warming for the intensification and weather of " 3R " problem to directly results in being greatly decreased for natural enemy quantity Regulating force declines, and the reproduction effect that pesticide produces makes plant hopper have outburst exacerbation trend.
With the strengthening of people's awareness of environmental protection, chemical prevention has not met the requirement of green agriculture gradually.To environment without Harmful biological control has manifested its superiority and importance.The light wing fleahopper Tytthus chinensis of China belong to Semiptera Miridae Miridae, light wing fleahopper belongs to Tytthus, main to prey on the plant hopper of Semiptera, leafhopper etc. in paddy ecosystem, is The important predator of planthopper.The light wing fleahopper of China is a kind of with moving property natural enemy, moves into rice field with planthopper, its field Between to originate population low, it is necessary to can just play control action to the later stage.
Since the quantity of natural enemy in nature is not enough to control pest, it is necessary to carry out indoor amount reproduction there are hysteresis quality, Then carry out Artificial Release and put capable prevention into.Although many grind is carried out to the artificial breeding of the light wing fleahopper of China at present Study carefully, the screening of food, but not matched prolific method are researched and developed, substituted to such as man-made feeds, especially It is not broken through in oviposition substrate, an indoor bottle for failing to carry out that amount reproduction is always the light wing fleahopper application of China Neck.
The content of the invention
In view of this, the present invention is directed to the problem of above-mentioned, there is provided a kind of method of the light wing fleahopper of artificial propagation China, this Invention is the light wing fleahopper of the carry out large scale artificial breeding of offer moisture and oviposition substrate China using kidney bean, without planting water Rice, it is easy to operate, cost is low, without season limit, it is suitable for applying during industry is promoted.
In order to solve the above-mentioned technical problem, the invention discloses a kind of method of the light wing fleahopper of artificial propagation China, including Following steps:
(1) glass culture dish for preparing a diameter of 13cm is used for the raising of the light wing fleahopper of China;
(2) filter paper of diameter 13cm is spread in culture dish;
(3) kidney bean, rice or degreasing cotton are prepared for feeding moisture and oviposition;
(4) food of the rice moth egg card as the light wing fleahopper of China is prepared;
(5) raising of the light wing fleahopper adult of China:Under conditions of 26-28 DEG C of temperature, humidity 65%-75%, by 45-55 The light wing fleahopper adult of head China is incorporated into step (1) ready culture dish, and it is ready that step (3) is put into culture dish Kidney bean, rice or degreasing cotton and the ready rice moth egg card of step (4), a kidney bean, rice or de- were replaced every 3 days Fat cotton, while rice moth egg card is replaced, and the rice moth egg card changed is abandoned;Kidney bean, the rice or de- changed The ovum of the substantial amounts of light wing fleahopper of China is had on fat cotton, by kidney bean, rice or degreasing cotton with the light wing fleahopper ovum of China Move into step (1) ready new culture dish, raised by step (6);
(6) raising of the light wing fleahopper nymph of China:Under conditions of 26-28 DEG C of temperature, humidity 65%-75%, by 1-2 bars Kidney bean, rice or degreasing cotton with the light wing fleahopper ovum of China are moved into step (1) ready culture dish, while in training Support in ware and be put into step (3) ready kidney bean, rice or degreasing cotton and the ready rice moth egg card of step (4), every 3 It replaces a kidney bean, rice or degreasing cotton, while replaces rice moth egg card, until the light wing fleahopper of China sprouts wings;Under replacement Rice moth egg card and kidney bean, the rice or degreasing cotton come abandons processing;After raising 8-14 days, by the Chinese light wing of emergence Fleahopper is chosen, and is raised by step (5).
Further, all kidney beans in the step (3) cut off 2 ends, make it to 10cm or so, while 2 ends are used Seal with wax mouth.
Further, the rice in the step (3) is handled in the following way:By single tiller of 30-45 days after transplanting Clean;The degreasing cotton parcel of root moistening;Rice strain is cut into scissors spare after 10cm.
Further, the preparation rice moth egg fixture body in the step (4) is:The rice moth egg of freezing is fixed by glue On A4 copy papers, correspondingly sized use is cut into according to demand after to be dried;Stand-by ovum card is stored into -20 DEG C of refrigerators.
Further, with 50-100 rice moth egg in the step (5):The ratio of the light wing fleahopper of 1 China launches ovum card For the light wing fleahopper imago feeding of China.
Further, with 100 rice moth eggs in the step (5):The ratio of the light wing fleahopper of 1 China is launched in the confession of ovum card The light wing fleahopper imago feeding of China.
Further, in the step (5) the light wing fleahopper of China adult insect density>50, nymph insect density>50 When, divide ware to raise.
Further, in the step (6) after the light wing fleahopper of China hatches 5 days, the insect density of nymph>50/training Support the raising of ware time-division ware.
Compared with prior art, the present invention can be obtained including following technique effect:
The present invention is the light wing fleahopper of the carry out large scale artificial breeding of offer moisture and oviposition substrate China using kidney bean, no It is easy to operate, cost is low, without season limit with rice cultivation, it is suitable for applying during industry is promoted.
Certainly, implement any of the products of the present invention and it is not absolutely required to reach all the above technique effect at the same time.
Embodiment
Carry out the embodiment that the present invention will be described in detail below in conjunction with embodiment, thereby to the present invention how application technology hand Section solves technical problem and reaches the process of realizing of technical effect to fully understand and according to this implement.
The present invention is the light wing fleahopper of the carry out large scale artificial breeding of offer moisture and oviposition substrate China using kidney bean.Institute The Chinese light wing fleahopper stated refers to the Chinese light wing fleahopper (Tytthus of Semiptera (Hemiptera) Miridae (Miridae) Chinensis), the rice moth refers to the rice moth in Lepidoptera (Lepidoptera) Galleriidae (Galleriidae) (Corcyra cephalonica), the kidney bean refer to the four seasons in beans mesh (Fabales) pulse family (Leguminosae) Beans (Phaseolus vulgaris).Comprise the following steps that:
(1) glass culture dish for preparing a diameter of 13cm is used for the raising of the light wing fleahopper of China;
(2) filter paper of diameter 13cm is spread in culture dish;
(3) prepare kidney bean for feeding moisture and oviposition, kidney bean is cut off into 2 ends, make it to 10cm or so, while 2 End mouth of sealing with wax;
(4) prepare food of the rice moth egg card as the light wing fleahopper of China, the rice moth egg of freezing is fixed on A4 by glue On copy paper, 3cm x 3cm uses are cut into after to be dried after to be dried.Stand-by ovum card can be stored into -20 DEG C of refrigerators;
(5) raising of the light wing fleahopper adult of China:Under conditions of 27 ± 1 DEG C of temperature, humidity 70 ± 5%, by 50 ± 10 The light wing fleahopper adult of head China is incorporated into step (1) ready culture dish, and it is ready that step (3) is put into culture dish Kidney bean and the ready rice moth egg card of step (4), with 100 rice moth eggs:The ratio of the light wing fleahopper of 1 China is launched ovum card and is supplied The light wing fleahopper imago feeding of China.A kidney bean and rice moth egg card were replaced every 3 days, and the rice moth egg card changed is thrown Abandon.The ovum of the substantial amounts of light wing fleahopper of China is had on the kidney bean changed, the kidney bean with the light wing fleahopper ovum of China is moved Enter in the ready new culture dish of step (1), raised by step (6);
(6) raising of the light wing fleahopper nymph of China:Under conditions of 27 ± 1 DEG C of temperature, humidity 70 ± 5%, by 1-2 bands The kidney bean for having the light wing fleahopper ovum of China is moved into step (1) ready culture dish, while step (3) is put into culture dish Ready kidney bean and the ready rice moth egg card of step (4), with 50-100 rice moth egg:The ratio of the light wing fleahopper of 1 China Example launches ovum card for the light wing fleahopper nymph feeding of China.A kidney bean and rice moth egg card were replaced every 3 days, until the light wing of China Fleahopper sprouts wings.The rice moth egg card and kidney bean changed abandons processing.After the light wing fleahopper of China hatches 5 days, nymph Insect density>50/culture dish time-division ware is raised.
After raising 8-14 days, the Chinese light wing fleahopper of emergence is chosen, is raised by step (5).
Embodiment 1 is with the light wing fleahopper of rice moth egg and kidney bean raising China
(1) glass culture dish for preparing a diameter of 13cm is used for the raising of the light wing fleahopper of China, and is spread in culture dish The filter paper of diameter 13cm;
(2) prepare kidney bean for feeding moisture and oviposition, kidney bean is cut off into 2 ends, make it to 10cm or so, while 2 End mouth of sealing with wax;
(3) prepare food of the rice moth egg card as the light wing fleahopper of China, the rice moth egg of freezing is fixed on A4 by glue On copy paper, correspondingly sized use is cut into according to demand after to be dried.Stand-by ovum card can be stored into -20 DEG C of refrigerators;
(4) raising of the light wing fleahopper adult of China:Under conditions of 27 ± 1 DEG C of temperature, humidity 70 ± 5%, by 50 ± 10 The light wing fleahopper adult of head China is incorporated into step (1) ready culture dish, and it is ready that step (2) is put into culture dish Kidney bean and the ready rice moth egg card of step (3), with 100 rice moth eggs:The ratio of the light wing fleahopper of 1 China is launched ovum card and is supplied The light wing fleahopper imago feeding of China.A kidney bean and rice moth egg card were replaced every 3 days, and the rice moth egg card changed is thrown Abandon.The ovum of the substantial amounts of light wing fleahopper of China is had on the kidney bean changed, the kidney bean with the light wing fleahopper ovum of China is moved Enter in the ready new culture dish of step (1), raised by step (5);
(5) raising of the light wing fleahopper nymph of China:Under conditions of 27 ± 1 DEG C of temperature, humidity 70 ± 5%, by 1-2 bands The kidney bean for having the light wing fleahopper ovum of China is moved into step (1) ready culture dish, while step (2) is put into culture dish Ready kidney bean and the ready rice moth egg card of step (3), with 50-100 rice moth egg:The ratio of the light wing fleahopper of 1 China Example launches ovum card for the light wing fleahopper nymph feeding of China.A kidney bean and rice moth egg card were replaced every 3 days, until the light wing of China Fleahopper sprouts wings.The rice moth egg card and kidney bean changed abandons processing.After the light wing fleahopper of China hatches 5 days, nymph Insect density>50/culture dish time-division ware is raised.
After raising 8-14 days, the Chinese light wing fleahopper of emergence is chosen, is raised by step (5).
Embodiment 2 is with the light wing fleahopper of rice moth egg and rice raising China
The present embodiment substitutes kidney bean as the light wing fleahopper moisture of offer China and production with rice as different from Example 1 The medium of ovum.Wherein rice prepares according to the following steps:
(1) single tiller of 30-45 days after transplanting is cleaned;
(2) the degreasing cotton parcel of root moistening;
(3) with scissors, by rice strain, to be cut into 10cm or so rear spare.
Embodiment 3 is with the light wing fleahopper of rice moth egg and degreasing cotton raising China
It is blind as the light wing of China is provided that the present embodiment substitutes kidney bean with the degreasing cotton of moistening as different from Example 1 Stinkbug moisture and the medium of oviposition.
Embodiment 4
Feeding effect with kidney bean, rice and degreasing cotton as the medium for providing the light wing fleahopper moisture of China and oviposition Experiment
According to the light wing of method raising China that the light wing fleahopper nymph of China is raised in embodiment 1, embodiment 2 and embodiment 3 Fleahopper, but it is simply placed in the nymph hatched at the beginning of one in each culture dish.Daily investigate record nymph survival condition and develop into Degree, until sprouting wings.Different media respectively does 100 repetitions.
After nymph sprouts wings, according to the method that the light wing fleahopper adult of China is raised in embodiment 1, embodiment 2 and embodiment 3 The light wing fleahopper adult of raising China, but the Chinese light wing fleahopper adult to sprout wings at the beginning of 1 pair is simply placed in each culture dish, investigate daily Female adult survival condition is recorded, while the ovum amount of the light wing fleahopper of China in the kidney bean that records under mirror is dug in solution, until female adult is dead. Different media respectively does 10 repetitions.
Nymph survival rate, development duration, female adult service life and the egg laying amount of the light wing fleahopper of China under 1 different substrates of table
Although the results show that by medium of kidney bean nymph survival rate, female adult service life and egg laying amount close to using rice as The effect of medium, is significantly higher than the effect using degreasing cotton as medium.But no matter kidney bean will compare in preparation and preservation Rice is convenient many, therefore more traditional Chinese light wing fleahopper raising of the invention is operationally easy to be very much, the behaviour having had The property made and economy.
Some preferred embodiments of invention have shown and described in described above, but as previously described, it should be understood that invention is not Form disclosed herein is confined to, is not to be taken as the exclusion to other embodiment, and available for various other combinations, modification And environment, and can be carried out in the scope of the invention is set forth herein by the technology or knowledge of above-mentioned teaching or association area Change., then all should be in power appended by invention and changes and modifications made by those skilled in the art do not depart from the spirit and scope of invention In the protection domain that profit requires.

Claims (8)

1. the method for the light wing fleahopper of a kind of artificial propagation China, it is characterised in that include the following steps:
(1) glass culture dish for preparing a diameter of 13cm is used for the raising of the light wing fleahopper of China;
(2) filter paper of diameter 13cm is spread in culture dish;
(3) kidney bean, rice or degreasing cotton are prepared for feeding moisture and oviposition;
(4) food of the rice moth egg card as the light wing fleahopper of China is prepared;
(5) raising of the light wing fleahopper adult of China:Under conditions of 26-28 DEG C of temperature, humidity 65%-75%, by 45-55 The light wing fleahopper adult of China is incorporated into step (1) ready culture dish, and step (3) the ready four seasons are put into culture dish Beans, rice or degreasing cotton and the ready rice moth egg card of step (4), a kidney bean, rice or absorbent cotton were replaced every 3 days Flower, while rice moth egg card is replaced, and the rice moth egg card changed is abandoned;Kidney bean, rice or the absorbent cotton changed The ovum for having the substantial amounts of light wing fleahopper of China is taken, the kidney bean with the light wing fleahopper ovum of China, rice or degreasing cotton are moved into In the ready new culture dish of step (1), raised by step (6);
(6) raising of the light wing fleahopper nymph of China:Under conditions of 26-28 DEG C of temperature, humidity 65%-75%, 1-2 bars are carried Kidney bean, rice or the degreasing cotton of the light wing fleahopper ovum of China are moved into step (1) ready culture dish, while in culture dish In be put into step (3) ready kidney bean, rice or degreasing cotton and the ready rice moth egg card of step (4), every 3 days more A kidney bean, rice or degreasing cotton are changed, while replaces rice moth egg card, until the light wing fleahopper of China sprouts wings;Change Rice moth egg card and kidney bean, rice or degreasing cotton abandon processing;After raising 8-14 days, by the Chinese light wing fleahopper of emergence Choose, raised by step (5).
2. the method for the light wing fleahopper of artificial propagation China according to claim 1, it is characterised in that in the step (3) All kidney beans cut off 2 ends, make it to 10cm or so, while 2 end mouths of sealing with wax.
3. the method for the light wing fleahopper of artificial propagation China according to claim 1, it is characterised in that in the step (3) Rice handle in the following way:Single tiller of 30-45 days after transplanting is cleaned;The degreasing cotton parcel of root moistening; Rice strain is cut into scissors spare after 10cm.
4. the method for the light wing fleahopper of artificial propagation China according to claim 1, it is characterised in that in the step (4) Preparation rice moth egg fixture body be:The rice moth egg of freezing is fixed on A4 copy papers by glue, it is to be dried after according to demand It is cut into correspondingly sized use;Stand-by ovum card is stored into -20 DEG C of refrigerators.
5. the method for the light wing fleahopper of artificial propagation China according to claim 1, it is characterised in that in the step (5) With 50-100 rice moth egg:The ratio of the light wing fleahopper of 1 China launches ovum card for the light wing fleahopper imago feeding of China.
6. the method for the light wing fleahopper of artificial propagation China according to claim 1, it is characterised in that in the step (5) With 100 rice moth eggs:The ratio of the light wing fleahopper of 1 China launches ovum card for the light wing fleahopper imago feeding of China.
7. the method for the light wing fleahopper of artificial propagation China according to claim 1, it is characterised in that in the step (5) The adult insect density of the light wing fleahopper of China>50, nymph insect density>When 50, ware is divided to raise.
8. the method for the light wing fleahopper of artificial propagation China according to claim 1, it is characterised in that in the step (6) After the light wing fleahopper of China hatches 5 days, the insect density of nymph>50/culture dish time-division ware is raised.
CN201711165330.6A 2017-11-21 2017-11-21 A kind of method of the light wing fleahopper of artificial propagation China Pending CN107926863A (en)

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CN110463666A (en) * 2019-09-09 2019-11-19 浙江省农业科学院 Expanding propagation method in Similis room
CN113261538A (en) * 2021-06-30 2021-08-17 中国热带农业科学院环境与植物保护研究所 Artificial breeding method of predatory stinkbug natural enemy cissa major eye stinkbug
CN113439712A (en) * 2020-03-24 2021-09-28 北京市农林科学院 Large-scale breeding method of orius sauteri with simple operation

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Publication number Priority date Publication date Assignee Title
CN110463666A (en) * 2019-09-09 2019-11-19 浙江省农业科学院 Expanding propagation method in Similis room
CN113439712A (en) * 2020-03-24 2021-09-28 北京市农林科学院 Large-scale breeding method of orius sauteri with simple operation
CN113261538A (en) * 2021-06-30 2021-08-17 中国热带农业科学院环境与植物保护研究所 Artificial breeding method of predatory stinkbug natural enemy cissa major eye stinkbug
CN113261538B (en) * 2021-06-30 2022-06-24 中国热带农业科学院环境与植物保护研究所 Artificial breeding method of predatory stinkbug natural enemy cissa major eye stinkbug

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Application publication date: 20180420