CN110463666A - Expanding propagation method in Similis room - Google Patents
Expanding propagation method in Similis room Download PDFInfo
- Publication number
- CN110463666A CN110463666A CN201910857206.9A CN201910857206A CN110463666A CN 110463666 A CN110463666 A CN 110463666A CN 201910857206 A CN201910857206 A CN 201910857206A CN 110463666 A CN110463666 A CN 110463666A
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- kidney bean
- oviposition
- ovum
- hatching
- minute pirate
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- 238000000034 method Methods 0.000 title claims abstract description 37
- 241001635552 Anthocoridae Species 0.000 claims abstract description 82
- 244000046052 Phaseolus vulgaris Species 0.000 claims abstract description 81
- 230000017448 oviposition Effects 0.000 claims abstract description 65
- 210000004681 ovum Anatomy 0.000 claims abstract description 55
- 102000002322 Egg Proteins Human genes 0.000 claims abstract description 54
- 108010000912 Egg Proteins Proteins 0.000 claims abstract description 54
- 230000012447 hatching Effects 0.000 claims abstract description 51
- 241001414989 Thysanoptera Species 0.000 claims abstract description 45
- 241000819999 Nymphes Species 0.000 claims abstract description 17
- 235000013305 food Nutrition 0.000 claims description 25
- 239000000758 substrate Substances 0.000 claims description 25
- 239000010455 vermiculite Substances 0.000 claims description 22
- 229910052902 vermiculite Inorganic materials 0.000 claims description 22
- 235000019354 vermiculite Nutrition 0.000 claims description 22
- 239000002023 wood Substances 0.000 claims description 12
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 10
- 239000002274 desiccant Substances 0.000 claims description 10
- 239000000741 silica gel Substances 0.000 claims description 10
- 229910002027 silica gel Inorganic materials 0.000 claims description 10
- 229960001866 silicon dioxide Drugs 0.000 claims description 10
- 239000011521 glass Substances 0.000 claims description 9
- 239000002994 raw material Substances 0.000 claims description 9
- 238000011534 incubation Methods 0.000 claims description 7
- 239000013589 supplement Substances 0.000 claims description 7
- 241000578422 Graphosoma lineatum Species 0.000 claims description 5
- 230000001954 sterilising effect Effects 0.000 claims description 5
- 238000004659 sterilization and disinfection Methods 0.000 claims description 5
- 238000005286 illumination Methods 0.000 claims description 2
- 238000009395 breeding Methods 0.000 abstract description 11
- 230000001488 breeding effect Effects 0.000 abstract description 11
- 241000238631 Hexapoda Species 0.000 abstract description 7
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- 238000011161 development Methods 0.000 abstract description 4
- 230000018109 developmental process Effects 0.000 abstract description 4
- 230000004083 survival effect Effects 0.000 abstract description 3
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- 230000035611 feeding Effects 0.000 description 9
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- 235000013339 cereals Nutrition 0.000 description 5
- 238000001035 drying Methods 0.000 description 5
- 241000576418 Jasminum nudiflorum Species 0.000 description 4
- 238000004140 cleaning Methods 0.000 description 4
- 235000013601 eggs Nutrition 0.000 description 4
- 229920001684 low density polyethylene Polymers 0.000 description 4
- 239000004702 low-density polyethylene Substances 0.000 description 4
- 235000021332 kidney beans Nutrition 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- 241001425390 Aphis fabae Species 0.000 description 2
- 241000143476 Bidens Species 0.000 description 2
- 235000010662 Bidens pilosa Nutrition 0.000 description 2
- 241000927584 Frankliniella occidentalis Species 0.000 description 2
- 241000545744 Hirudinea Species 0.000 description 2
- 241001635529 Orius Species 0.000 description 2
- 241000607479 Yersinia pestis Species 0.000 description 2
- 230000003796 beauty Effects 0.000 description 2
- 238000004851 dishwashing Methods 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 244000062645 predators Species 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 239000004575 stone Substances 0.000 description 2
- 239000008399 tap water Substances 0.000 description 2
- 235000020679 tap water Nutrition 0.000 description 2
- 241001124076 Aphididae Species 0.000 description 1
- 241001414720 Cicadellidae Species 0.000 description 1
- 241000122098 Ephestia kuehniella Species 0.000 description 1
- 235000010469 Glycine max Nutrition 0.000 description 1
- 244000068988 Glycine max Species 0.000 description 1
- 241000255777 Lepidoptera Species 0.000 description 1
- 241001647846 Orius insidiosus Species 0.000 description 1
- 241001635524 Orius strigicollis Species 0.000 description 1
- 206010041954 Starvation Diseases 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Housing For Livestock And Birds (AREA)
Abstract
The present invention provides a kind of indoor expanding propagation methods of Similis, are related to technical field of insect feeding.The method of the invention includes the collection of ovum, the hatching of ovum and nymph raising, adult are collected and the refrigeration of ovum.The oviposition carrier of the host plant for raising thrips and minute pirate bugs is both configured to kidney bean beanpod in the method for the invention, simplifies raising step, facilitates the development of minute pirate bugs large-scale breeding.Humidity can be reduced by 10% or so in the padding that centers of oviposition dress by the present invention, with improve adult survival rate and ovum quantity；It arranges in pairs or groups in hatching the lids of different hollow out diameters, hatching rate can be improved 20%；The adult of suction be put into oviposition device before quickly mate in culture dish in advance, can by single kidney bean ovum quantity improve 105%.Using the method for the invention, the ovum of obtained minute pirate bugs is refrigerated into 8d in 8 DEG C of environment, moreover it is possible to guarantee the hatching rate of ovum up to 70% or more, adult collection rate about 30%.
Description
Technical field
The invention belongs to technical field of insect feeding, and in particular to expanding propagation method in Similis room.
Background technique
Similis (Oriusstrigicollis) has the characteristics that predatism is wide, adaptable, to thrips, aphid,
Tetranychid, aleyrodid, leafhopper, lepidoptera pest ovum and newly hatched larvae have apparent control action, and one kind has a extensive future
Predator.And solve the problems, such as the interior expansion of Similis it is numerous be key link that its field is able to utilize.
Currently, having the report of prolific technology and method in Similis room both at home and abroad, such as Ito is using ground
The ovum of middle sea powder snout moth's larva is as food, soybean seedling as oviposition substrate mass propagation Similis (ItoK.A indoors
simple mass-rearing method forpredaceous Orius bugs in the laboratory.Applied
Entomology and Zoology,2007,42(4):573-577)；Bueno etc. selects glass jar and corrugated board as south
The feeding environment condition of square minute pirate bugs, equally also using the ovum of Mediterranean flour moth as food, the flower of beggar-ticks is as oviposition substrate
And moisture source, establish extensive feeding system (Bueno V, Mendes SM, Carvalho in Similis room
LM.Evaluation of a rearing-method for the predator Orius insidiosus.Bulletin
of Insectology,2006,59(1):1-6)；Zhang Changrong etc. then Frankliniella occidentalis and black bean aphid are combined feed south it is small
Flower stinkbug, for winter jasmine tender stem as oviposition medium (patent authorization number: CN105325377B), Zhou Xingmiao etc. is also by winter jasmine spray
As oviposition medium, food is then the man-made feeds (application number: CN201510397419.X) independently prepared, and Zheng Li etc. is then proposed
A method of raising Orius, i.e., using gelechiid ova (can the factorial production) as main feed, using kidney bean beanpod as
Main oviposition substrate (application number: 201620176955.7).
Currently, in the also jejune situation of Similis man-made feeds, raising on a large scale the most key is food
Object and oviposition substrate, and food is essentially insect living body, oviposition substrate is then plant tissue.It is conceivable that if as south
The insect of minute pirate bugs food, the host plant of feeding are just also the suitable oviposition substrate of Similis, then can simplify ring
It saves and reduces cost.For example, if feeding Similis, beggar-ticks or winter jasmine as oviposition with Frankliniella occidentalis or black bean aphid
Medium then needs the link prepared to have, 1) raising as the insect of food, needs to prepare in advance host's plant of this kind of insect institute feeding
Object, such as bean；2) cultivation as the winter jasmine of oviposition substrate.Above-mentioned link at least prepares 2-3 kind plant and 3 kinds
The raising of insect, a link are fallen, then will lead to the stagnation of Similis large-scale breeding.
Summary of the invention
In view of this, the purpose of the present invention is to provide expanding propagation methods in a kind of Similis room, hence it is evident that simplify raising
Link, save the cost, and the egg hatching rate of the minute pirate bugs after refrigerating are 70% or more, and adult collection rate is 30% or so.
In order to achieve the above-mentioned object of the invention, the present invention the following technical schemes are provided:
The present invention provides expanding propagation methods in Similis room, comprising the following steps: (1) bottom from bottom to top successively
Tiling has in the minute pirate bugs of the first padding and filter paper oviposition device, the oviposition substrate that tiles on the filter paper and the rue for carrying food source
Beans beanpod is launched minute pirate bugs adult, is cultivated after closeing the lid；First padding includes the raw material of following parts by weight: leech
340~360 parts of stone, 42~48 parts of wood shavings sawdust and 36~45 parts of silica-gel desiccant；The oviposition substrate is the rue cleaned up
Beans beanpod；180~220 thrips nymphs are carried on each kidney bean beanpod in the kidney bean beanpod for carrying food source；
The kidney bean beanpod for carrying minute pirate bugs ovum is collected within (2) second days, and supplements fresh kidney bean beans identical with quantity is collected
Pod and food source；
(3) the kidney bean beanpod for carrying minute pirate bugs ovum is placed in hatching apparatus, carries out hatching and nymph culture, until
There is adult appearance, then adult taking-up is placed in culture dish daily and is mated；
(4) the minute pirate bugs adult of post-coitum is placed in the minute pirate bugs oviposition device, collects minute pirate bugs ovum and refrigerates guarantor
It deposits.
Preferably, step (1) minute pirate bugs oviposition device includes round angle glass preserving jar, long 16cm, width 22cm,
High 23cm, angle bore are 12cm, and cover diameter is 13cm, volume 4.3L；The minute pirate bugs oviposition device is using preceding warp
High-temperature sterilization treatment；The intermediate hollow-out part of the lid is the circle of diameter 9cm, and the gauze of 100 mesh of hollow-out part is continuously
Gap is clung.
Preferably, the quantity ratio of the kidney bean beanpod of step (1) oviposition substrate and carrying food source is 3:1；The little Hua
The male and female quantity ratio of stinkbug adult is 2:1.
Preferably, the temperature of step (1) described culture is 27 DEG C, humidity 70%, illumination: the dark time is 14h:
10h。
Preferably, step (2) the collected method clamps kidney bean including the use of tweezers, and shakes off the thrips on kidney bean
And minute pirate bugs adult.
Preferably, step (3) hatching apparatus is identical as minute pirate bugs oviposition device, in the second padding of bottom tiling
And filter paper；Second padding includes the raw material of following parts by weight: 340~360 parts and 42~48 parts of wood shavings sawdust of vermiculite.
Preferably, in step (3) hatching and nymph incubation, according to the different phase of hatching, difference is used
Lid: starting in 3d hollow-out part among the lid that uses in the hatching is the circle of diameter 5cm, and hollow-out part is with 100
Purpose gauze gapless is clung；4d starts, then changes the circle that intermediate hollow-out part is diameter 9cm, 100 mesh of hollow-out part into
Gauze gapless cling.
Preferably, in the hatching and nymph incubation, since the 4d of the hatching, addition 1 is taken daily
Kidney bean beanpod with 180~220 thrips nymphs；8d starts, and addition 2 carries 180~220 thrips nymphs daily
Kidney bean beanpod.
Preferably, adult places quantity no more than 15 in step (3) described culture dish, and standing time is within 2h.
Preferably, step (4) the stored refrigerated temperature is 4~8 DEG C, and the stored refrigerated time is 5~8d.
The present invention provides a kind of indoor expanding propagation methods of Similis, the hatching of collection, ovum including ovum and nymph
Raising, adult are collected and the refrigeration of ovum.The raising step of the thrips as feed is simplified in the method for the invention, simultaneously will
The raising of thrips and minute pirate bugs and oviposition carrier are both configured to kidney bean beanpod, facilitate the development of minute pirate bugs large-scale breeding:
(1) padding of the invention that tiles in oviposition device, the matched combined of the padding is more scientific and reasonable, effectively control temperature
Humidity, especially humidity；
(2) hatching apparatus of the present invention is arranged in pairs or groups the lids of different hollow out diameters in hatching, can be improved hatching rate and
Survival rate；
(3) in hatching and nymph cultivation stage, the interim quick post-coitum in culture dish after adult is sucked out places into oviposition
In device, promote mating using the small space of culture dish, hence it is evident that improve efficiency；
(4) when the interior expansion for carrying out minute pirate bugs using the method for the invention is numerous, by the ovum of obtained minute pirate bugs in 8 DEG C of rings
8d is refrigerated in border, moreover it is possible to guarantee the hatching rate of ovum up to 70% or more, adult collection rate nearly 30%.
Detailed description of the invention
Fig. 1 is minute pirate bugs oviposition device；
Fig. 2 is thrips Breeding container；
Fig. 3 is oviposition substrate preparation stage figure；
Fig. 4 is that minute pirate bugs adult quickly mates figure in culture dish；
Fig. 5 is that minute pirate bugs produce the ovum on kidney bean beanpod.
Specific embodiment
The present invention provides expanding propagation methods in Similis room, comprising the following steps: (1) bottom from bottom to top successively
Tiling has in the minute pirate bugs of the first padding and filter paper oviposition device, the oviposition substrate that tiles on the filter paper and the rue for carrying food source
Beans beanpod is launched minute pirate bugs adult, is cultivated after closeing the lid；First padding includes the raw material of following parts by weight: leech
340~360 parts of stone, 42~48 parts of wood shavings sawdust and 36~45 parts of silica-gel desiccant；The oviposition substrate is the rue cleaned up
Beans beanpod；180~220 thrips nymphs are carried on each kidney bean beanpod in the kidney bean beanpod for carrying food source；
The kidney bean beanpod for carrying minute pirate bugs ovum is collected within (2) second days, and supplements fresh kidney bean beans identical with quantity is collected
Pod and food source；
(3) the kidney bean beanpod for carrying minute pirate bugs ovum is placed in hatching apparatus, carries out hatching and nymph culture, until
There is adult appearance, then adult taking-up is placed in culture dish daily and is mated；
(4) the minute pirate bugs adult of post-coitum is placed in the minute pirate bugs oviposition device, collects minute pirate bugs ovum and refrigerates guarantor
It deposits.
In expanding propagation method of the present invention, successively tiling in bottom has the minute pirate bugs oviposition device of the first padding and filter paper
Interior, the oviposition substrate that tiles on the filter paper and the kidney bean beanpod for carrying food source are launched minute pirate bugs adult, are carried out after closeing the lid
Culture；First padding includes the raw material of following parts by weight: 340~360 parts of vermiculite, 42~48 parts of wood shavings sawdust and silica gel are dry
36~45 parts of drying prescription；The oviposition substrate is the kidney bean beanpod cleaned up；Each rue in the kidney bean beanpod for carrying food source
Beans beanpod carries 180~220 thrips nymphs.Minute pirate bugs oviposition device of the present invention is preferably round angle as shown in Figure 1
Glass preserving jar, long 16cm, width 22cm, high 23cm, angle bore are 12cm, and cover diameter is 13cm, volume 4.3L.This hair
Preferably through high-temperature sterilization treatment before using, the high temperature sterilization preferably dries at 105 DEG C the bright minute pirate bugs oviposition device
2h.The specification of filter paper of the present invention is preferably 20 × 20cm.Minute pirate bugs oviposition device of the present invention further includes lid, described
The intermediate hollow-out part of lid is preferably the circle of diameter 9cm, and the gauze gapless of 100 mesh of hollow-out part is clung.The present invention
The raw material of first padding preferably includes: 345~355 parts of vermiculite, 43~46 parts of wood shavings sawdust and silica-gel desiccant 38~42
Part, more preferably including 350 parts of vermiculite, 45 parts of wood shavings sawdust and 40 parts of silica-gel desiccant.Vermiculite of the present invention is preferably partial size
For the bulky grain vermiculite of 1~3mm.The matched combined of padding of the present invention is more scientific and reasonable, can effectively control epidemic disaster,
Especially humidity can be reduced by 10% or so, to guarantee not will form steam in oviposition device, be unlikely to drowned adult or rue
Beans are overly wet and interfere the selection of Adult worms producting eggs position
Oviposition substrate of the present invention is the drying kidney bean beanpod cleaned up, the preparation method of the oviposition substrate, with
It for 30 beanpods, preferably includes: 30 beanpods being placed in 2L clear water, the dish washing liquid of 0.15mL is added, is then floated
It washes, beanpod of holding with a firm grip in water washes away 30s repeatedly up and down, rinses 15s under tap water after lifting beanpod completely；By washed beanpod by
It is a to be layered on filter paper, the water droplet on beanpod is gently wiped using water suction paper handkerchief, then in two cross air blasting of beanpod.It is of the present invention to blow
Wind is preferably the direction for being blowed using the electric fan of beauty, and beanpod gap position need to being aligned to wind, to ensure that beanpod stitches
Gap position is dry and comfortable, blow-time 30min, and temperature is 28 DEG C.The power of electric fan of the present invention is preferably 30~50w, more
Preferably 40w.The method of cleaning of the present invention can wash off beanpod chemical agent remained on surface.
For food source of the present invention preferably by the kidney bean beanpod of thrips feeding 5d or more, the preparation method of the food source is preferred
The following steps are included: setting thrips Breeding container, basin mouth diameter 26cm, volume 4L；Bulky grain vermiculite is placed in basin, weight is
110g, a qualitative filter paper (25*25cm) of tiling on vermiculite；Breeding container basin lid is thickness 0.5cm, the circle of diameter 33cm is organic
Glass, the circular hole that basin lid centre hollow out diameter is 22cm, hollow-out part are clung with 100 mesh gauze gaplesss.In each Breeding container
8 kidney bean beanpods that are fresh and having cleaned are put into, about 200 childhood thrips and 100 adult thrips is put into, observes thrips daily
Quantity simultaneously supplements 2 kidney beans that are fresh and having cleaned, clears up in thrips Breeding container daily and turns to be yellow rotten kidney bean and be put into thrips
And in minute pirate bugs recover, the thrips and minute pirate bugs recover are preferably as shown in Chinese patent CN201821795513.6.
Oviposition substrate of the present invention and the quantity ratio for carrying the kidney bean beanpod of food source are preferably 3:1, that is, device of laying eggs
Tile 3 oviposition substrates on filter paper, while placing the food source (kidney bean beanpod) for carrying certain amount thrips above it.
The amount of the minute pirate bugs adult of dispensing of the present invention is preferably 15/oviposition device, and the sex ration of the minute pirate bugs adult is preferred
It for 2:1, closes the lid rapidly, is cultivated later.The temperature of culture of the present invention is preferably 27 DEG C, humidity 70%, light
According to: dark time ratio is 14h:10h.In the present invention, 3 oviposition substrates can be paved with filter paper just, be unlikely to excessively to gather around
It squeezes, is conducive to that spawning efficiency is made to reach highest；And there are about 200 or so thrips on every kidney bean beanpod, are 15 minute pirate bugs
One day amount, not will cause minute pirate bugs inanition, the waste of thrips will not be generated.
The present invention collected the kidney bean beanpod for carrying minute pirate bugs ovum in second day, and supplemented fresh rue identical with quantity is collected
Beans beanpod and food source.The method of collection of the present invention preferably includes to clamp kidney bean using tweezers, and shakes off the thrips on kidney bean
And minute pirate bugs adult can further be brushed using writing brush if quantity is more.The present invention requires supplementation with same after having collected
The fresh kidney bean of quantity need to add same amount of thrips as supplement daily.It is also preferable to include utilizations for collection of the present invention
Pooter clears up the corpse of minute pirate bugs daily, when minute pirate bugs adult is less than 5 in spawning tank, then discards the cylinder, resets.
The kidney bean beanpod for carrying minute pirate bugs ovum is placed in by the present invention after collecting the kidney bean beanpod for carrying minute pirate bugs ovum
In hatching apparatus, hatching and nymph culture are carried out, until there is adult appearance, adult is taken out using pest sucking device be placed in training daily
It supports and mates in ware.Hatching apparatus of the present invention is preferably identical as minute pirate bugs oviposition device, the tiling second in bottom
Padding and filter paper；Second padding includes the raw material of following parts by weight: 340~360 parts and 42~48 parts of wood shavings sawdust of vermiculite.
The present invention is in the hatching and nymph incubation, according to the different phase of hatching, it is preferable to use different lids, to mention
High hatchability and survival rate: start the circle that hollow-out part among the lid used in 3d is diameter 5cm, hollow out in the hatching
The gauze gapless of 100 mesh of part clings (hollow out diameter is small to be conducive to improve humidity in cylinder, in favor of the hatching of ovum)；4d is opened
Begin, then changes the circle that intermediate hollow-out part is diameter 9cm into, the gauze gapless of 100 mesh of hollow-out part, which is clung, (need to reduce cylinder
Interior humidity, humidity is excessively high to be easy to cause water droplet).The present invention preferably is selected from the hatching in the hatching and nymph incubation
4d start, the kidney bean beanpod that addition 1 carries 200 thrips nymphs daily；8d starts, 2 carryings of addition daily
There is the kidney bean beanpod of 200 thrips nymphs.The present invention is in the hatching and nymph incubation, and it is also preferable to include daily cleanings
It dries up and mouldy kidney bean and is put into thrips and minute pirate bugs recover in hatching tank, the thrips and minute pirate bugs recover are preferably such as
Shown in Chinese patent CN201821795513.6.The adult is momentarily placed in culture dish by the present invention, in the culture dish at
Worm places quantity and is preferably more than 15, and standing time is within 2h, using the small space of culture dish, with promote minute pirate bugs at
Worm mates in advance before being put into oviposition tank.The raw material of second padding of the present invention preferably includes: 345~355 parts of vermiculite and
43~46 parts of wood shavings sawdust, more preferably include 350 parts and 45 parts of wood shavings sawdust of vermiculite.Vermiculite of the present invention is preferably that partial size is
The bulky grain vermiculite of 1~3mm.
The minute pirate bugs adult of the post-coitum is placed in the minute pirate bugs oviposition device by the present invention, collects minute pirate bugs ovum simultaneously
It is stored refrigerated.The collection method of minute pirate bugs ovum of the present invention preferably includes 10 carryings are small by taking 10 kidney bean beanpods as an example
The kidney bean of flower stinkbug ovum is put into low density polyethylene (LDPE) freshness protection package (25*30cm), and to guarantee beanpod beans nose part outwardly；Then again
Freshness protection package is put into plastic casing (25*35cm), plastic casing is then placed in refrigerator and saves.Stored refrigerated temperature of the present invention
Preferably 4~8 DEG C, more preferably 8 DEG C of degree.The stored refrigerated time of the present invention is preferably 5~8d, more preferably 8d.
Expanding propagation method in Similis room provided by the invention is described in detail below with reference to embodiment, still
They cannot be interpreted as limiting the scope of the present invention.
Embodiment 1
1, the raising of thrips
It is arranged thrips Breeding container (as shown in Figure 2): basin mouth diameter 26cm, volume 4L；Bulky grain vermiculite, weight are placed in basin
Amount is 110g, a qualitative filter paper (25*25cm) of tiling on vermiculite；Breeding container basin lid is the circle of thickness 0.5cm, diameter 33cm
Organic glass, the circular hole that basin lid centre hollow out diameter is 22cm, hollow-out part are clung with 100 mesh gauze gaplesss.Each raising
It is put into 8 kidney bean beanpods that are fresh and having cleaned in basin, is put into about 200 childhood thrips and 100 adult thrips, observes daily
Thrips quantity simultaneously supplements 2 kidney beans that are fresh and having cleaned.It turns to be yellow and rotten kidney bean and is put into cleaning thrips Breeding container daily
In thrips and minute pirate bugs recover.
2, the collection of ovum
1) setting oviposition tank (as shown in Figure 1): oviposition tank is a round angle glass preserving jar, long 16cm, width 22cm, height
23cm, angle bore are cm, and cover diameter is cm, volume 4.3L；Tile padding for spawning tank bottom, and padding group becomes bulky grain
(Kunshan wasion desiccant is limited for vermiculite (1-3mm, weight 350g), wood shavings sawdust (weight 45g) and two bags of 20g silica-gel desiccants
Company's production), high-temperature sterilization treatment (105 DEG C of baking 2h) is both needed to before use；Qualitative filter paper (20*20cm) is tiled above padding.
2) prepare oviposition substrate (as shown in Figure 3): oviposition substrate is commercially available fresh, unabroken kidney bean beanpod, length
It is not particularly limited with width.30 beanpods are placed in 2L clear water, the dish washing liquid of 0.15mL is added, is then rinsed,
Beanpod of holding with a firm grip in water washes away 30s repeatedly up and down, rinses 15s under tap water after lifting beanpod completely.One by one by washed beanpod
It is layered on filter paper, gently wipes the water droplet on beanpod using water suction paper handkerchief, it is then (described using the electric fan of beauty in beanpod two sides
The power of electric fan is preferably 30~50w, more preferably 40w) it is blowed, and beanpod gap position need to be aligned to the side of wind
To ensure that beanpod gap position is dry and comfortable, blow-time 30min, temperature is 28 DEG C.
3) setting oviposition: 3 kidney beans are placed in tiling above the filter paper of each oviposition tank, and are put into 1 and are carried 200 Jis
The kidney bean (guaranteeing the kidney bean by thrips feeding 5d or more) of horse nymph；15 little Hua are blown into using pooter in each oviposition tank
Stinkbug adult, it is female, male than being 2:1, cover the cover of repacking, i.e., the circle that intermediate hollow out diameter is 9cm, hollow-out part rapidly later
It is clung with the gauze gapless of 100 mesh.The temperature of culture is 27 DEG C, humidity 70%, and the photoperiod (L:D) is 14:10.
4) collection of ovum.Collect the kidney bean for having carried minute pirate bugs ovum every other day, collection mode be kidney bean is clamped using tweezers, and
The thrips and minute pirate bugs adult shaken off on kidney bean can further be brushed using writing brush if quantity is more.It is supplemented after having collected
Same amount of fresh kidney bean need to add same amount of thrips as supplement daily.Minute pirate bugs are cleared up daily using pooter
Corpse then discard the cylinder when minute pirate bugs adult is less than 5 in spawning tank, reset.
3, the hatching of ovum and nymph raising
Minute pirate bugs are set and hatch tank, ovum is hatched in 3d after giving birth to substantially.Hatching tank be with spawning tank as glass
Glass cylinder, but hatch in tank and do not place silica-gel desiccant, there are also be exactly the cover style difference used in the different time stage.Each incubate
Change the kidney bean that tank places 8 carrying Similis ovum, it is the circle that intermediate hollow out diameter is 5cm, hollow out that cover is used in 3d
The gauze gapless of 100 mesh of part is clung.4d starts, then changes the circle that intermediate hollow out diameter is 9cm into, and hollow-out part is used
The gauze gapless of 100 mesh is clung, and adds 1 kidney bean for carrying 200 thrips nymphs；8d starts, then adds 2 and take
Kidney bean with 200 thrips nymphs.Daily cleaning, which is hatched, dries up mouldy kidney bean and is put into thrips and minute pirate bugs recycling in tank
In device.
4, adult is collected
There is situation in minute pirate bugs adult in daily observation hatching tank, if being found immediately with pooter be sucked out to
With.The minute pirate bugs adult of suction is temporarily placed in plastic culture dish (diameter 9cm, as shown in Figure 4), and the control of adult quantity exists
Within 15 (male worm is not less than 5), standing time is no more than 2h.By minute pirate bugs adult in plastic culture dish according to regulation quantity
It is transferred in the oviposition tank newly set, and carries out oviposition link, oviposition is as shown in Figure 5.
5, the refrigeration of ovum
The tolerance to cold of ovum is most strong, best worm state when being refrigeration.Part Similis ovum is refrigerated, with reasonable
Population quantity in Similis room is controlled, and is supplemented when needed, Similis expansion can be evaded well
" supply shortage " risk occurred during numerous.The kidney bean that 10 carry minute pirate bugs ovum is put into low density polyethylene (LDPE) freshness protection package (25*
In 30cm), and to guarantee beanpod beans nose part outwardly；Then freshness protection package is put into plastic casing (25*35cm) again, plastic casing is then
It is placed in refrigerator and saves.The refrigerated condition of Similis ovum and refrigeration time limit are respectively 8 DEG C and 8d, can guarantee the hatching of ovum
Rate is up to 70% or more, adult collection rate nearly 30%.
In 1 implementation process of embodiment, also carried out influence (50 repetition) of the silica-gel desiccant for temperature and humidity in cylinder,
Ovum and nymphal development go through phase (15 weights in influence (5 repetitions) of the different style covers to the hatching of minute pirate bugs ovum, hatching cylinder
The hatching rate (5d) of ovum and adult collection rate (20d) (15 repetitions) etc. are tested again) and under different refrigerated conditions, as a result such as table
Shown in 1~5:
Table 1 adds influence of the silica-gel desiccant to epidemic disaster in cylinder
| Processing | Mean temperature (DEG C) | Medial humidity (%RH) |
| Empty cylinder | 27.5 | 76.9 |
| Vermiculite+sawdust | 29.0 | 75.7 |
| Vermiculite+sawdust+1 is responsible for a task until it is completed drying prescription | 27.9 | 78.9 |
| Vermiculite+sawdust+2 is responsible for a task until it is completed drying prescription | 27.9 | 68.3 |
| Vermiculite+sawdust+3 is responsible for a task until it is completed drying prescription | 28.0 | 67.2 |
Influence of the different style covers of table 2 to the hatching of minute pirate bugs ovum
| Processing | The average percentage hatch rate (%) of ovum |
| 5cm hollow out diameter | 92.6 |
| 9cm hollow out diameter | 72.5 |
Ovum and nymphal development go through the phase in the hatching cylinder of table 3
| Worm state | Averagely go through the phase (d) |
| Ovum | 2.8 |
| 1 age nymph | 2.5 |
| 2 age nymphs | 1.7 |
| 3 age nymphs | 1.7 |
| 4 age nymphs | 1.5 |
| 5 age nymphs | 2.9 |
| Non- puberty (total) | 13.1 |
The hatching rate (5d) and adult collection rate (20d) of ovum under the different refrigerated conditions of table 4
The influence that 5 minute pirate bugs adult of table quickly mates for effect of laying eggs
Note: female, male worm ratio is 2:1 (total 15), and kidney bean beanpod 3, other feedings are consistent.
The present invention provides expanding propagation methods in a kind of Similis room, simplify raising link and reduce cost, will obtain
Minute pirate bugs ovum refrigerate 8d under the conditions of 8 DEG C, moreover it is possible to guarantee the hatching rate of ovum up to 70% or more, adult collection rate nearly 30%.
The above is only a preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications are also answered
It is considered as protection scope of the present invention.
Claims (10)
1. expanding propagation method in Similis room, comprising the following steps: (1) in bottom, successively tiling has the first padding from bottom to top
In the minute pirate bugs oviposition device of filter paper, the oviposition substrate that tiles on the filter paper and the kidney bean beanpod for carrying food source are launched small
Flower stinkbug adult, carries out oviposition culture after closeing the lid；First padding includes the raw material of following parts by weight: vermiculite 340~360
Part, 42~48 parts of wood shavings sawdust and 36~45 parts of silica-gel desiccant；The oviposition substrate is the kidney bean beanpod cleaned up；It is described
It carries and carries 180~220 thrips nymphs in the kidney bean beanpod of food source on each kidney bean beanpod；
Collect within (2) second days the kidney bean beanpod for carrying minute pirate bugs ovum, and supplement with the identical fresh kidney bean beanpod of collection quantity and
Food source；
(3) the kidney bean beanpod for carrying minute pirate bugs ovum is placed in hatching apparatus, carries out hatching and nymph culture, until have into
Worm occurs, then adult taking-up is placed in culture dish daily and is mated；
(4) the minute pirate bugs adult of post-coitum is placed in the minute pirate bugs oviposition device, collection minute pirate bugs ovum is simultaneously stored refrigerated.
2. method according to claim 1, which is characterized in that step (1) the minute pirate bugs oviposition device includes round angle
Glass preserving jar, long 16cm, width 22cm, high 23cm, angle bore are 12cm, and cover diameter is 13cm, volume 4.3L；It is described
Minute pirate bugs oviposition device is before using through high-temperature sterilization treatment；The intermediate hollow-out part of the lid is the circle of diameter 9cm, is engraved
The gauze gapless of 100 mesh of empty part is clung.
3. method according to claim 1, which is characterized in that step (1) oviposition substrate and the kidney bean beans for carrying food source
The quantity ratio of pod is 3:1；The male and female quantity ratio of the minute pirate bugs adult is 2:1.
4. method according to claim 1, which is characterized in that the temperature of step (1) described culture is 27 DEG C, and humidity is
70%, illumination: the dark time is 14h:10h.
5. method according to claim 1, which is characterized in that step (2) collected method is clamped including the use of tweezers
Kidney bean, and shake off the thrips on kidney bean and minute pirate bugs adult.
6. method according to claim 1, which is characterized in that step (3) hatching apparatus and minute pirate bugs oviposition fill
Set identical, the second padding of tiling and filter paper in bottom；Second padding includes the raw material of following parts by weight: vermiculite 340~360
Part and 42~48 parts of wood shavings sawdust.
7. method according to claim 1, which is characterized in that in step (3) hatching and nymph incubation, according to
The different phase of hatching uses different lids: starting hollow-out part among the lid used in 3d in the hatching is diameter
The gauze gapless of the circle of 5cm, 100 mesh of hollow-out part is clung；4d starts, then changing intermediate hollow-out part into is diameter
The gauze gapless of the circle of 9cm, 100 mesh of hollow-out part is clung.
8. method according to claim 7, which is characterized in that in the hatching and nymph incubation, from the hatching
4d start, the kidney bean beanpod that addition 1 carries 180~220 thrips nymphs daily；8d starts, daily addition 2
Carry the kidney bean beanpod of 180~220 thrips nymphs.
9. method according to claim 1, which is characterized in that adult places quantity and is no more than in step (3) described culture dish
15, standing time is within 2h.
10. method according to claim 1, which is characterized in that step (4) the stored refrigerated temperature is 4~8 DEG C, cold
The time that hiding saves is 5~8d.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114794019A (en) * | 2021-06-01 | 2022-07-29 | 浙江省农业科学院 | Method for breeding orius miri |
| US11489265B2 (en) | 2019-07-11 | 2022-11-01 | Nanjing University Of Posts And Telecommunications | Reconfigurable wideband phase-switched screen based on artificial magnetic conductor |
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| CN101331868A (en) * | 2008-08-07 | 2008-12-31 | 北京市农林科学院 | Artificial propagation method of minute pirate bugs |
| CN104938429B (en) * | 2015-06-30 | 2017-04-05 | 山东省农业科学院植物保护研究所 | A kind of method for raising Orius |
| CN107926863A (en) * | 2017-11-21 | 2018-04-20 | 浙江省农业科学院 | A kind of method of the light wing fleahopper of artificial propagation China |
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| JP2001346481A (en) * | 2001-04-11 | 2001-12-18 | Sumitomo Chem Co Ltd | Method for preparing amblyseius womersleyi schicha not exhibiting short-day reproductive diapause and method for controlling insect pest using amblyseius womersleyi schicha |
| CN101331868A (en) * | 2008-08-07 | 2008-12-31 | 北京市农林科学院 | Artificial propagation method of minute pirate bugs |
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| CN114794019A (en) * | 2021-06-01 | 2022-07-29 | 浙江省农业科学院 | Method for breeding orius miri |
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