CN105940873A - A method for acquiring pepper pollen - Google Patents

A method for acquiring pepper pollen Download PDF

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Publication number
CN105940873A
CN105940873A CN201610356981.2A CN201610356981A CN105940873A CN 105940873 A CN105940873 A CN 105940873A CN 201610356981 A CN201610356981 A CN 201610356981A CN 105940873 A CN105940873 A CN 105940873A
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China
Prior art keywords
pollen
casing
paper
pepper
flower pesticide
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CN201610356981.2A
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Chinese (zh)
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CN105940873B (en
Inventor
邹学校
梁成亮
周书栋
杨博智
杨莎
戴雄泽
马艳青
李雪峰
张竹青
陈文超
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INST OF VEGETABLES HUNAN PROV
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INST OF VEGETABLES HUNAN PROV
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Priority to CN201610356981.2A priority Critical patent/CN105940873B/en
Publication of CN105940873A publication Critical patent/CN105940873A/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01DHARVESTING; MOWING
    • A01D93/00Harvesting apparatus not provided for in other groups of this subclass

Abstract

The invention provides a method for acquiring pepper pollen. The method comprises the steps of 1) collecting pepper male flowers which have whitish buds and do not bloom; 2) picking anthers off the pepper male flowers; 3) acquiring pollen from the anthers via a pollen collecting box: placing the anthers in the pollen collecting box, closing the box body, and keeping the box body at a proper temperature and humidity to promote the anthers to scatter pollen; 4) taking out a paper sheet, sieving a mixture on the paper sheet and obtaining the pepper pollen. With the method, high quality pepper pollen can be obtained and high yield of pepper pollen can be guaranteed; the method solves the problems of insufficient pollen production and reduction of pollen viability caused by technical restriction and weather influence in conventional pepper hybrid seed production.

Description

A kind of method obtaining capsicum pollens
Technical field
The present invention relates to a kind of method obtaining capsicum pollens, particularly to the design of a kind of pollen collection case, Yi Jitong The method crossing the pollen collection case effective acquisition capsicum pollens of design, belongs to Fructus Capsici cross-breeding technology field.
Background technology
During Fructus Capsici cross breeding seed, affect pollen quantity and quality because have: the Parent florescence is sterile, plantation Proportional imbalance, rainy weather, pollen preparation technique are poor.Pollen quantity meeting not enough and of low quality causes the yield of hybrid seed to drop Low, cost increases, and seeding farmer and production of hybrid seeds enterprise profit reduce or loss, slacken the market competition of kind and enterprise further Power.It is therefore desirable to carry out the efficient preparation method research of capsicum pollens.
Plant pollen preparation method, its technology generally comprises following step: it is good that (1) plant full-bloom stage gathers enough growths Good male flower;(2) flower pesticide is peeled off from floral organ;(3) pollen is departed from from flower pesticide;(4) pollen is used for after drying breeding And production.
Summary of the invention
In order to overcome the not enough and low-quality problem of pollen quantity during Fructus Capsici cross breeding seed, it is an object of the invention to It is to provide a kind of high efficiency to obtain high-quality capsicum pollens method, and the method material requested is simple with instrument, draw materials conveniently, With low cost, solving is affected by technical limitations and weather in Fructus Capsici cross breeding seed that the powder delivery that causes is not enough, Pollen Activity drops Low problem.
In order to realize above-mentioned technical purpose, the invention provides a kind of method obtaining capsicum pollens, the method include with Lower step:
1) gather petal to turn white but unopened Fructus Capsici male flower;
2) flower pesticide of described Fructus Capsici male flower is peeled off;
3) described flower pesticide obtains pollen by pollen collection case;
Described pollen collection case includes casing, and described box bottom lays calculogenesis grieshoch, and described calculogenesis grieshoch upper berth has The scraps of paper;Described casing top is provided with heating and dehumidification equipment;
Described flower pesticide is placed on the described scraps of paper in pollen collection case, containment housing, opens heating and dehumidification equipment, control Spin manifold temperature is 28~35 DEG C, and humidity, 40~50%, promotes flower pesticide loose powder;
4) taking out the scraps of paper, sieved by the mixture on the scraps of paper, sieve takes capsicum pollens.
Technical scheme, devises a kind of casing being particularly suitable for obtaining capsicum pollens, and box bottom is provided with stone Grieshoch, on the one hand lkd layer for moisture absorption, keeps the humidity environment in casing, on the other hand acts on microorganism, prevent pollen Souring ferment.Calculogenesis grieshoch upper berth has the scraps of paper, the scraps of paper to be mainly used in collecting pollen.Calculogenesis grieshoch also can guarantee that the dry of the scraps of paper simultaneously Dry, heating and dehumidification equipment is mainly casing and provides thermal source, keeps the temperature in casing, also has certain exsiccation simultaneously, with Humidity in quick lime regulating box body together, casing under normal working conditions, can keep the condition in casing to reach Fructus Capsici The optimum condition of flower pesticide loose powder, promotes pepper anther loose powder.
Preferably scheme, the time opening the process of heating and dehumidification equipment is 18~24h.Casing normally work 20 hours left The right side, flower pesticide the most all realizes loose powder.
More preferably scheme, heating and dehumidification equipment is electric filament lamp.Electric filament lamp mainly provides thermal source, also has certain simultaneously Exsiccation.
Preferably scheme, casing is rectangular structure, and casing can be opened in upper bottom surface or at least one side of described casing; It is easy to being removed and placed into of case substance in vivo.
More preferably scheme, casing is paper material.Paper box body is used to be conducive to the maintenance of temperature and humidity in casing.
Preferably 40 °~50 ° inclinations of screen cloth are repeated sieve during sieving and take 2~3 times by scheme.
More preferably scheme, sieve employing 80 mesh pollen sieve.
Preferably scheme, the flower pesticide after loose powder uses hairbrush to rub brush back and forth with the hands, makes the pollen being bonded on flower pesticide come off.Improve flower The yield of powder.
Rational height in casing of the electric filament lamp watt level of the present invention, electric filament lamp and casing size, according to reality Situation suitably regulates, and only needs the temperature and humidity in control chamber body to meet and requires.
Hinge structure, the beneficial effect that technical scheme is brought:
The method obtaining capsicum pollens of the present invention is based primarily upon pollen collection case and realizes, and pollen collection case is simultaneously to heating And dehumidifying has carried out designing cleverly, maintain suitable temperature and humidity environment, may advantageously facilitate the quick loose powder of flower pesticide, simultaneously Ensure that the vigor of pollen, it is thus achieved that high yield, high-quality capsicum pollens;Solve in existing Fructus Capsici cross breeding seed by technology Limit and weather affects powder delivery deficiency, the problem of Pollen Activity reduction caused.
The present invention produces that the method material requested of capsicum pollens is simple with instrument, draws materials conveniently, with low cost.
Accompanying drawing explanation
[Fig. 1] is the structure diagram of pollen collection case:
Wherein,
1 is casing, and 2 is electric filament lamp, and 3 is flower pesticide, and 4 is the scraps of paper, and 5 make a living lkd layer.
Detailed description of the invention
Following example are intended to further illustrate present invention rather than limit the model of the claims in the present invention protection Enclose.
The box sizes that following example use is 44cm × 28cm × 32cm, and electric filament lamp power is 40w.
Quick lime is placed in carton bottom, smooth on quick lime is placed with the smooth paper without pasta, the Fructus Capsici of stripping Medicine is laid in the shiny surface of paper, and then the electric filament lamp with heating function be suspended from the top of carton, bulb and flower pesticide away from From 30cm, bulb is suitable with all flower pesticide distance, thermally equivalent.When after the whole loose powder of the flower pesticide on paper, then with pollen sieve sieve Go out pollen.When producing pollen, pollen sieve and 45 degree of angles of pollen Pengcheng, pollen flows down from sieve aperture, and impurity rolls into the pollen sieve end Portion, after repeating 2-3 time, staying the pollen in pollen basin is pure pure pollen.Now use or take certain mode to preserve Standby.
Embodiment 1
This experimental examination incandescent bulb prescribed distance and illumination duration are on the impact of humiture in carton.Illumination distances sets respectively Being set to 10cm, 20cm, 30cm, every 0.5h measures a temperature and humidity, respectively places a temperature with corner in the middle of carton bottom Drimeter.As it can be seen from table 1 incandescent lighting has heating and wet down effect.Illumination distances is the shortest, and paper the temperature inside the box is the highest And it is the fastest to heat up, humidity is the highest.When distance is for 30cm, it is heated in carton and air humidity is more uniform, after illumination 2.5h, paper The stable little ecosystem of temperature 28 DEG C, humidity 44% has been defined inside case.
Table 1 incandescent bulb prescribed distance and illumination duration are on the impact (2016.5.5) of humiture in carton
Embodiment 2
This experimental check illumination distances impact on Pollen Activity.Electric filament lamp with carton with case study on implementation 1, test pollen is The vigor of freezen protective is the capsicum pollens of 85%.Bulb and pollen distance respectively 10cm, 20cm, 30cm, ck be without Illumination process, other arrange identical.A length of 3h during illumination, measures Pollen Activity after 3h.Pollen Activity detection method is TTC dyeing Method.Three repetitions are done in each process.Result of the test shows, 40 watt lamp illumination 3h, safe distance is more than 30cm, common paper The height of case i.e. can meet.
The distance of table 2 electric filament lamp and the flower pesticide impact (2016.5.5 on Pollen Activity.Room temperature 24 DEG C, humidity 78%)
Embodiment 3
The temperature that this experimental check incandescent lighting duration produces affects on flower pesticide loose powder effect and on Pollen Activity.Incandescent 40 watt lamp common on market selected by lamp, the pollen that pollen has just dissipated from flower pesticide, carton volume 44 × 28 × 32cm.Lamp Bubble and pollen distance are 30cm.Pollen Activity detection method is TTC staining.Three repetitions are done in each process.Experimental result table Bright, when bulb and pollen distance are for 30cm, increase illumination duration and do not increase the temperature inside the box, within 24h loose powder rate 90% with On, Pollen Activity does not has significant change.
Table 3 illuminates duration to the impact of powder delivery and Pollen Activity (2015.5.23, Changsha, 27 DEG C)
Embodiment 4
This experimental check quick lime coordinates bulb that temperature, pollen in case are dissipated effect, the impact of Pollen Activity.Experiment knot Fruit shows, the water sorption of quick lime ensure that being dried and powder delivery effect of pollen, and in the case of not having quick lime, heating dissipates Powder, can cause pollen souring ferment, vigor to reduce further;Bulb has heating and wet down effect, has loose powder speed one fixing Ring.
Table 4 Calx, bulb on the impact of powder delivery and Pollen Activity (2014.12.23, Hainan, rain, ambient temperature 17 DEG C, Humidity 75%)

Claims (8)

1. the method obtaining capsicum pollens, it is characterised in that: comprise the following steps:
1) gather petal to turn white but unopened Fructus Capsici male flower;
2) flower pesticide of described Fructus Capsici male flower is peeled off;
3) described flower pesticide obtains pollen by pollen collection case;
Described pollen collection case includes casing, and described box bottom lays calculogenesis grieshoch, and there are the scraps of paper on described calculogenesis grieshoch upper berth; Described casing top is provided with heating and dehumidification equipment;
Described flower pesticide is placed on the described scraps of paper in pollen collection case, containment housing, opens heating and dehumidification equipment, control casing Temperature is 28~35 DEG C, and humidity, 40~50%, promotes flower pesticide loose powder;
4) taking out the scraps of paper, sieved by the mixture on the scraps of paper, sieve takes capsicum pollens.
The method of acquisition capsicum pollens the most according to claim 1, it is characterised in that: open what heating and dehumidification equipment processed Time is 18~24h.
The method of acquisition capsicum pollens the most according to claim 2, it is characterised in that: described heating and dehumidification equipment is white Vehement lamp.
4. according to the method obtaining capsicum pollens described in any one of claims 1 to 3, it is characterised in that: described casing is Rectangular structure, casing can be opened in upper bottom surface or at least one side of described casing.
The method of acquisition capsicum pollens the most according to claim 4, it is characterised in that: described casing is paper material.
The method of acquisition capsicum pollens the most according to claim 1, it is characterised in that: described sieve during by screen cloth 40 °~50 ° of inclinations repeat sieve and take 2~3 times.
The method of acquisition capsicum pollens the most according to claim 6, it is characterised in that: the described employing 80 mesh pollen that sieve Sieve.
The method of acquisition capsicum pollens the most according to claim 1, it is characterised in that: the flower pesticide after loose powder uses hairbrush Return and rub brush with the hands, make the pollen being bonded on flower pesticide come off.
CN201610356981.2A 2016-05-26 2016-05-26 A kind of method for obtaining capsicum pollens Expired - Fee Related CN105940873B (en)

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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106605499A (en) * 2016-12-21 2017-05-03 新疆生产建设兵团第二师农业科学研究所 A physical absorption purification method for pear pollen
CN109105251A (en) * 2018-08-22 2019-01-01 张东风 A method of improving grape artificial pollination success rate
CN110089276A (en) * 2019-06-11 2019-08-06 江西省科学院生物资源研究所 A kind of Collection and conservation method of Kiwi fruit pollen
CN113465298A (en) * 2021-07-16 2021-10-01 四川农业大学 South Asia loquat pollen drying and storing method
CN113853938A (en) * 2021-09-08 2021-12-31 三明市农业科学研究院 Pepper pollen fetching and storing method

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CN104365286A (en) * 2014-09-10 2015-02-25 宁波市鄞州云帆工程咨询有限公司 Non-injuring-type pollen pini collection method
CN204443180U (en) * 2015-01-29 2015-07-08 赤峰市农牧科学研究院 Marigold pollen harvester
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CN203399532U (en) * 2013-08-05 2014-01-22 甘肃省农垦农业研究院 Marigold pollen collection device
CN203633179U (en) * 2013-12-24 2014-06-11 山西省农业科学院玉米研究所 Maize pollen collector
CN104067787A (en) * 2014-06-26 2014-10-01 临泽县康裕种苗有限责任公司 Pollen extractor
CN104206125A (en) * 2014-09-10 2014-12-17 宁波市鄞州云帆工程咨询有限公司 Gingko pollen collecting method
CN104365286A (en) * 2014-09-10 2015-02-25 宁波市鄞州云帆工程咨询有限公司 Non-injuring-type pollen pini collection method
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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106605499A (en) * 2016-12-21 2017-05-03 新疆生产建设兵团第二师农业科学研究所 A physical absorption purification method for pear pollen
CN109105251A (en) * 2018-08-22 2019-01-01 张东风 A method of improving grape artificial pollination success rate
CN110089276A (en) * 2019-06-11 2019-08-06 江西省科学院生物资源研究所 A kind of Collection and conservation method of Kiwi fruit pollen
CN113465298A (en) * 2021-07-16 2021-10-01 四川农业大学 South Asia loquat pollen drying and storing method
CN113853938A (en) * 2021-09-08 2021-12-31 三明市农业科学研究院 Pepper pollen fetching and storing method

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