CN105265293A - Milletia speciosa champ seed rapid propagation method - Google Patents
Milletia speciosa champ seed rapid propagation method Download PDFInfo
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- CN105265293A CN105265293A CN201510749361.0A CN201510749361A CN105265293A CN 105265293 A CN105265293 A CN 105265293A CN 201510749361 A CN201510749361 A CN 201510749361A CN 105265293 A CN105265293 A CN 105265293A
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- river sand
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/02—Germinating apparatus; Determining germination capacity of seeds or the like
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
Abstract
The invention discloses a milletia speciosa champ seed rapid propagation method. The method includes the following steps that: 80 to 90 parts by weight of 20 to 40 mesh river sand and 10 to 20 parts by weight of 10 to 18 mesh river sand are fully mixed, and the mixture is heated for 15 to 20mins in a drying oven under 145 to 155 DEG C, and the mixture stands and cools, and clean water is added into the mixture so as to wet the mixture, and therefore, treated river sand can be obtained; the treated river sand is laid at a box with holes formed at the bottom, and the thickness of the river sand is more than 3cm, and soaked milletia speciosa champ seeds are uniformly scattered on river sand, and a layer of treated river sand of 1 to 2cm thickness is laid on the soaked milletia speciosa champ seeds; and the box filled with the seeds is irradiated by an infrared heat preservation lamp for 10 to 24 hours per day with temperature controlled from 25 to 30 DEG C, the rest hours in each day, the box filled with the seeds is arranged under an environment of 25 to 30 DEG C, so that germination acceleration can be carried out, and the seeds are watered for once each day. With the method adopted, after 5 to 7 days of germination acceleration, the seeds can be germinated, and the germination rate of the seeds can be up to 98%.
Description
Technical field
The present invention relates to technical field of plant propagation, specifically a kind of ox seed method for quickly breeding energetically.
Background technology
Ox is (MillettiaspeciosaChamp.) energetically, is pulse family Millettia plant, calls ox, beautiful millettia root, blood rattan, Kowloon beading, Tianniudali, energetically potato etc. energetically.Ox is used as medicine with root energetically, and Main Ingredients and Appearance is protein, starchiness and alkaloid etc.Ox is the sweet perfume (or spice) of smell energetically, warm in nature and, there is establishing-Yang, support kidney qi-restoratives, strong muscle is active, and effect of flat liver, moistening lung, cures mainly and suffer from a deficiency of the kidney, the deficiency of vital energy, pain in low back and legs, rheumatism, chronic hepatitis, bronchitis, cough, the curative effects such as pulmonary tuberculosis.The ground such as Guangdong, Guangxi usually carrys out Baoshang with Niu great Li block root and eats, and it has medicinal and edible value concurrently.Because Niu great Li has very large using value, the market demand increases severely in recent years. and wild resource is through excavating for many years energetically for ox, and reserves reduce gradually, and produce and pharmaceutical raw material supply trend anxiety, price constantly rises.Therefore, Niu great Li propagation technique is study hotspot in recent years.
Traditional Niu great Li sapling multiplication technology mainly contains following several: breeding is cultivated in seminal propagation, cottage propagation and group training.Seminal propagation method and sexual propagation method, utilize male and female to be pollinated exactly intersect and form seed to raise up seed, the advantage of seminal propagation once sows to obtain a large amount of nursery stock, seed collection, storage, convenient transportation, seedling growth is vigorous, strong stress resistance, easily tames.But there is following defect in traditional Niu great Li seminal propagation: (1) Niu great Li seed resource is in short supply, expensive, batch capture difficulty; (2) germination time is long, is generally 20-30 days; (3) percentage of seedgermination is low, only reaches about 45% etc.Namely cottage propagation gets a part for plant nutrition organ, and insert in loose wetting soil or fine sand, utilize its regeneration capacity, make it to take root and branch out, become new plant, its existing problem is: (1) cottage propagation survival rate is low, even all dead; (2) cutting resource scarcity, batch breeding difficulty etc.Tissue culture propagating is in synthetic medium, in vitro tissue cell chulture is made to become the propagation method of whole plant, be adopt the mode of callus induction, Calli Differentiation propagation and adventitious bud rooting to obtain aseptic seedling at present, this mode Problems existing is: (1) evoked callus process time is longer; (2) Calli Differentiation is that the frequency of indefinite bud is lower, and the indefinite bud aberration rate differentiated improves; (3) the Elongation of adventitious bud speed that goes out of Calli Differentiation is slow, and the time reaching the standard of taking root is long.Current ox energetically propagation technique Problems existing seriously hinders the large-scale production of Niu great Li, thus cannot meet market to ox demand energetically, and must set up can the method for a large amount of, Fast-propagation Niu great Li seedling.
Summary of the invention
The present invention is directed to the defect that Niu great Li seminal propagation technology exists, a kind of easy and simple to handle, quick, with low cost, seed propagation method that germination rate is high is provided, Niu great Li seedling large-scale breeding can be realized.
For realizing above object, the present invention is achieved by the following technical solutions:
A kind of ox seed method for quickly breeding energetically, comprises the following steps:
1. Niu great Li seed dries in the shade, and removes the seed that prematurity, particle are not full, the seed screened is put into the 50% carbendazol wettable powder soaking disinfection that concentration is 0.1%, be filtered dry;
2., with the Niu great Li seed 15-20h that 25-30 DEG C of emerge in worm water disinfects, be filtered dry;
3. take river sand according to following parts by weight: 20-40 order river sand 80-90 part, 10-18 order river sand 10-20 part, mix, heat 15-20min, cool in the baking oven of 145-155 DEG C, add clear water and make to soak, the river sand that must handle well;
4. be layered on by the river sand handled well in the porose chest in bottom, the thickness of river sand is more than 3cm, by soaked ox energetically seed be evenly sprinkling upon above river sand, repave the thick river sand handled well of one deck 1-2cm;
5. the infrared heat-insulating lamp of the chest described in step 4 is irradiated 10-24h/ days, control temperature is 25-30 DEG C, every day remaining time to be placed in temperature be that 25-30 DEG C of environment carries out vernalization, water every day 1 time.
20-40 order river sand described in step 3 refers to by 20 mesh standard sieves, not by the river sand of 40 mesh standard sieves; Described 10-18 order river sand refers to by 10 mesh standard sieves, not by the river sand of 18 mesh standard sieves.
Preferably, infrared heat-insulating lamp described in step 5 can be the heat-insulating lamp that can send near infrared ray [wavelength is between (0.75-1) ~ (2.5-3) μm], middle infrared (Mid-IR) [wavelength is between (2.5-3) ~ (25-40) μm] or far infrared [wavelength is (25-40) ~ l000 μm], preferred far infrared thermal insulation lamp.
Preferably, infrared heat-insulating lamp described in step 5 preferably can the heat-insulating lamp of A.T.C, when temperature reaches setting, automatically closes infrared heat-insulating lamp, when temperature is lower than setting, automatically opens infrared heat-insulating lamp.
For saving space, cost, ox energetically seed can place layer 2-3, namely the above step 4 is: be layered on bottom chest by the river sand handled well, the thickness of river sand is 3-4cm, by soaked ox energetically seed be evenly sprinkling upon above river sand, repave the thick river sand handled well of one deck 1cm and cover seed, layer 2-3 so repeatedly, the thick river sand handled well of paving one deck 1-2cm above of the superiors Niu great Li seed.
Compared with prior art, the beneficial effect that the present invention obtains is:
1. ox of the present invention energetically seed method for quickly breeding vernalization 5-7 days seeds can sprout; within 15 days, germination rate can reach 98%; germination is fast, germination rate is high; solve the problems such as Niu great Li seed propagation seedling cost is in the past high, the vernalization cycle long, germination rate is lower, for Niu great Li scale, industrialization plantation provide Reliable guarantee.
2. ox of the present invention energetically seed method for quickly breeding adopt infrared heat-insulating lamp, fully electric energy can be converted to infrared rays, infrared rays has stronger radiant force and penetration, there is significant temperature control effect and resonance effects, can produce with the hydrone of Niu great Li seed tissue cell and resonate, effectively promote seed sprouting growth.
3. ox of the present invention energetically seed method for quickly breeding select the river sand of different-grain diameter as sprouting matrix, there is well ventilative and water penetration, sufficient oxygen can be provided for seed sprouting, Niu great Li seed sprouting can be accelerated and improve germination rate.
4. ox of the present invention energetically seed method for quickly breeding operation is simple, with low cost, as there is no booth and air-conditioned room, also rapidly and efficiently vernalization can be completed with infrared heat-insulating lamp, the most multipotency of the inventive method place in a chest 3 layers of ox energetically seed carry out vernalization, making full use of resource, is a kind of method being particularly suitable for peasant's ectogenesis Niu great Li.
Embodiment
Test Niu great Li seed collection used in Guangxi Wuming County, below in conjunction with specific embodiment, the present invention is further described, but protection scope of the present invention is not limited only to embodiment.
One, Niu great Li seed method for quickly breeding condition is investigated
1. river sand granularity is investigated.
Ventilative and the water penetration of river sand is comparatively strong, applicable ox seed growth energetically.The present invention's same a pile river sand retracted from river bank, investigate the impact on ox seminal propagation energetically of different grain size river sand, be grouped as follows:
A group: the river sand retracted from river bank is crossed 20 mesh standard sieves, gets the river sand by 20 mesh standard sieves.
B group: the river sand retracted from river bank is crossed 40 mesh standard sieves, gets the river sand by 40 mesh standard sieves.
C group: the river sand retracted from river bank is crossed 20 mesh standard sieves, gets river sand by 20 mesh standard sieves after 40 mesh standard sieves, gets not by the river sand of 40 mesh standard sieves.
D group: the river sand retracted from river bank is crossed 10 mesh standard sieves, gets river sand by 10 mesh standard sieves after 18 mesh standard sieves, gets not by the river sand of 18 mesh standard sieves.
E group: get above-mentioned C group 85 parts, above-mentioned D group 15 parts, mixes.
By above-mentioned river sand, adopt and test with the following method: (1) Niu great Li seed dries in the shade, remove the seed that prematurity, particle are not full, the seed screened is put into the 50% carbendazol wettable powder soaking disinfection that concentration is 0.1%, be filtered dry; (2) with the Niu great Li seed 20h that 28 DEG C of emerge in worm water disinfect, be filtered dry; (3) get above-mentioned grouping river sand, in the baking oven of 150 DEG C, heat 15min, cool, add clear water and make to soak, the river sand that must handle well; (4) be layered on by the river sand handled well in the porose chest in bottom, the thickness of river sand is 3cm, by soaked ox energetically seed be evenly sprinkling upon above river sand, repave the thick river sand handled well of one deck 1cm; (5) chest described in step (4) is placed in transparent greenhouse, daytime normal solar light irradiation, control temperature is at 28 DEG C, and irradiate 12h/ days with infrared heat-insulating lamp in the evening, and control temperature, at 28 DEG C, waters 1 time every day.Often organize and parallelly carry out 3 tests, the results detailed in Table 1.
The river sand of table 1 different-grain diameter is on the impact of ox seed sprouting energetically
| Group | Beginning germinating time (my god) | 15 days average germination percentage (%) |
| A | 12 | 76 |
| B | 10 | 70 |
| C | 8 | 82 |
| D | 20 | 0 |
| E | 6 | 95 |
Above-mentioned result of the test shows, the impact of river sand granularity on ox seed germination is energetically comparatively large, and granularity is too thin, and gas permeability is bad, and granularity is too thick, and moisture capacity is bad, and E group river sand water conservation, air permeable effect are better, and seed germination is fast, and within 15 days, calculating germination rate is high.
2. illumination is investigated.
To investigate the impact of different illumination conditions on ox seed germination energetically in test, be grouped as follows:
A group: do not have illumination, chest is placed in dark place control temperature.
B group: be placed in control temperature in transparent greenhouse, daytime normal solar light irradiation.
C group: be placed in control temperature in transparent greenhouse, daytime normal solar light irradiation, night incandescent lamp irradiate 10h/ days.
D group: be placed in control temperature in transparent greenhouse, daytime normal solar light irradiation, night turns off air-conditioning, with far infrared thermal insulation light irradiation insulation 10h/ days.
E group: with far infrared thermal insulation light irradiation insulation 24h/ days.
Adopt and test with the following method: (1) Niu great Li seed dries in the shade, remove the seed that prematurity, particle are not full, the seed screened is put into the 50% carbendazol wettable powder soaking disinfection that concentration is 0.1%, be filtered dry; (2) with the Niu great Li seed 15h that 25-30 DEG C of emerge in worm water disinfects, be filtered dry; (3) river sand is taken according to following parts by weight: 20-40 order river sand 80 parts, 10-18 order river sand 20 parts, mix, and heats 15min, cool in the baking oven of 155 DEG C, adds clear water and make to soak, the river sand that must handle well; (4) be layered on by the river sand handled well in the porose chest in bottom, the thickness of river sand is 4cm, by soaked ox energetically seed be evenly sprinkling upon above river sand, repave the thick river sand handled well of one deck 2cm; (5) chest of step (4) is placed in above-mentioned different illumination condition and carries out vernalization, control temperature is 26 DEG C, waters every day 1 time.Often organize and parallelly carry out 3 tests, the results detailed in Table 2.
Table 2 different illumination conditions is on the impact of ox seed sprouting energetically
| Group | Beginning germinating time (my god) | 15 days average germination percentage (%) |
| a | 18 | 0 |
| b | 13 | 60 |
| c | 11 | 65 |
| d | 7 | 96 |
| e | 6 | 97 |
Above-mentioned result of the test shows, the impact of illumination condition on ox seed germination is energetically comparatively large, and suitable illumination condition can promote seed germination, and as d, e group, germinating time early, calculates germination rate in 15 days high.
Two, Niu great Li seed method for quickly breeding
Embodiment 1
A kind of ox seed method for quickly breeding energetically, comprises the following steps:
1. Niu great Li seed dries in the shade, and removes the seed that prematurity, particle are not full, the seed screened is put into the 50% carbendazol wettable powder soaking disinfection 15min that concentration is 0.1%, be filtered dry;
2., with the Niu great Li seed 15h that 25 DEG C of emerge in worm water disinfect, be filtered dry;
3. take river sand according to following parts by weight: 20-40 order river sand 80 parts, 10-18 order river sand 10 parts, mix, and heats 20min, cool in the baking oven of 145 DEG C, adds clear water and make to soak, the river sand that must handle well;
4. be layered on by the river sand handled well in the porose chest in bottom, the thickness of river sand is 3cm, by soaked ox energetically seed be evenly sprinkling upon above river sand, repave the thick river sand handled well of one deck 2cm;
5. the chest described in step 4 is placed in transparent greenhouse, daytime normal solar light irradiation, control temperature is at 25 DEG C, and night turns off air-conditioning, with near-infrared heat-insulating lamp irradiate insulation 10h/ days, control temperature, at 25 DEG C, waters 1 time every day.7th day seed starts to germinate, and within 15 days, germination rate reaches 94%.
Embodiment 2
A kind of ox seed method for quickly breeding energetically, comprises the following steps:
1. Niu great Li seed dries in the shade, and removes the seed that prematurity, particle are not full, the seed screened is put into the 50% carbendazol wettable powder soaking disinfection 15min that concentration is 0.1%, be filtered dry;
2., with the Niu great Li seed 20h that 28 DEG C of emerge in worm water disinfect, be filtered dry;
3. take river sand according to following parts by weight: 20-40 order river sand 90 parts, 10-18 order river sand 20 parts, mix, and heats 15min, cool in the baking oven of 155 DEG C, adds clear water and make to soak, the river sand that must handle well;
4. the river sand handled well is layered on bottom chest, the thickness of river sand is 4cm, by soaked ox energetically seed be evenly sprinkling upon above river sand, repave the thick river sand handled well of one deck 1cm and cover seed, so 3 layers repeatedly, the thick river sand handled well of paving one deck 1cm above of the superiors Niu great Li seed.
5. be placed in cubicle by the chest described in step 4, in using, infrared heat-insulating lamp irradiates 24h/ days, and control temperature is 30 DEG C, waters every day 1 time.Within 6th day, seed starts to sprout, and within 15 days, germination rate reaches 96%.
Embodiment 3
A kind of ox seed method for quickly breeding energetically, comprises the following steps:
1. Niu great Li seed dries in the shade, and removes the seed that prematurity, particle are not full, the seed screened is put into the 50% carbendazol wettable powder soaking disinfection 15min that concentration is 0.1%, be filtered dry;
2., with the Niu great Li seed 18h that 28 DEG C of emerge in worm water disinfect, be filtered dry;
3. take river sand according to following parts by weight: 20-40 order river sand 85 parts, 10-18 order river sand 15 parts, mix, and heats 18min, cool in the baking oven of 150 DEG C, adds clear water and make to soak, the river sand that must handle well;
4. be layered on by the river sand handled well in the porose chest in bottom, the thickness of river sand is 5cm, by soaked ox energetically seed be evenly sprinkling upon above river sand, repave the thick river sand handled well of one deck 2cm;
5. be placed in air-conditioned room by the chest described in step 4, control temperature is at 28 DEG C, and night turns off air-conditioning, and with far infrared thermal insulation light irradiation insulation 15h/ days, control temperature, at 28 DEG C, waters 1 time every day.5th day seed starts to germinate, and within 15 days, germination rate reaches 98%.
Embodiment 4
A kind of ox seed method for quickly breeding energetically, comprises the following steps:
1. Niu great Li seed dries in the shade, and removes the seed that prematurity, particle are not full, the seed screened is put into the 50% carbendazol wettable powder soaking disinfection 20min that concentration is 0.1%, be filtered dry;
2., with the Niu great Li seed 15h that 30 DEG C of emerge in worm water disinfect, be filtered dry;
3. take river sand according to following parts by weight: 20-40 order river sand 85 parts, 10-18 order river sand 15 parts, mix, and heats 15min, cool in the baking oven of 155 DEG C, adds clear water and make to soak, the river sand that must handle well;
4. be layered on by the river sand handled well in the porose chest in bottom, the thickness of river sand is 4cm, by soaked ox energetically seed be evenly sprinkling upon above river sand, repave the thick river sand handled well of one deck 2cm;
5. step 4 filled above seed-bearing chest and cover with airtight case, 60cm place, the top far infrared thermal insulation light irradiation insulation 24h/ days of the superiors' river sand, control temperature 30 DEG C, waters 1 every day.6th day seed starts to germinate, and within 15 days, germination rate reaches 93%.
Above content is in conjunction with concrete preferred embodiment further description made for the present invention, can not assert that specific embodiment of the invention is confined to these explanations.For general technical staff of the technical field of the invention; make some equivalent alternative or obvious modification without departing from the inventive concept of the premise; and performance or purposes identical, all should be considered as belonging to the scope of patent protection that the present invention is determined by submitted to claims.
Claims (2)
1. an ox seed method for quickly breeding energetically, is characterized in that, comprise the following steps:
(1) Niu great Li seed dries in the shade, and removes the seed that prematurity, particle are not full, the seed screened is put into the 50% carbendazol wettable powder soaking disinfection that concentration is 0.1%, be filtered dry;
(2) with the Niu great Li seed 15-20h that 25-30 DEG C of emerge in worm water disinfects, be filtered dry;
(3) river sand is taken according to following parts by weight: 20-40 order river sand 80-90 part, 10-18 order river sand 10-20 part, mix, heat 15-20min, cool in the baking oven of 145-155 DEG C, add clear water and make to soak, the river sand that must handle well;
(4) be layered on by the river sand handled well in the porose chest in bottom, the thickness of river sand is more than 3cm, by soaked ox energetically seed be evenly sprinkling upon above river sand, repave the thick river sand handled well of one deck 1-2cm;
(5) the infrared heat-insulating lamp of the chest described in step (4) is irradiated 10-24h/ days, control temperature is 25-30 DEG C, every day remaining time to be placed in temperature be that 25-30 DEG C of environment carries out vernalization, water every day 1 time.
2. ox according to claim 1 seed method for quickly breeding energetically, is characterized in that: described infrared heat-insulating lamp is far infrared thermal insulation lamp.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106856703A (en) * | 2017-03-01 | 2017-06-20 | 防城港市防城区蚕种场 | A kind of beautiful millettia root seed seedling method on scale |
| CN107736210A (en) * | 2017-11-10 | 2018-02-27 | 中国医学科学院药用植物研究所海南分所 | A kind of beautiful millettia root seedling efficient propagation method |
| CN107771629A (en) * | 2017-10-09 | 2018-03-09 | 广西吉朋投资有限公司 | A kind of method of beautiful millettia root seed winter Pregermination and seedling breeding |
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|---|---|---|---|---|
| CN106856703A (en) * | 2017-03-01 | 2017-06-20 | 防城港市防城区蚕种场 | A kind of beautiful millettia root seed seedling method on scale |
| CN107771629A (en) * | 2017-10-09 | 2018-03-09 | 广西吉朋投资有限公司 | A kind of method of beautiful millettia root seed winter Pregermination and seedling breeding |
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