CN105104172A - Method for solving flowering asynchronism of pitayas - Google Patents
Method for solving flowering asynchronism of pitayas Download PDFInfo
- Publication number
- CN105104172A CN105104172A CN201510527021.3A CN201510527021A CN105104172A CN 105104172 A CN105104172 A CN 105104172A CN 201510527021 A CN201510527021 A CN 201510527021A CN 105104172 A CN105104172 A CN 105104172A
- Authority
- CN
- China
- Prior art keywords
- pollen
- dragon fruit
- pollination
- temperature
- bottle
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Abstract
The invention discloses a method for solving flowering asynchronism of pitayas. Although an artificial pollination technology adopted in present production is relatively developed, reports for long-period storage of pitaya pollen are few. The method comprises the following steps: collecting the pollen; drying the pollen at 28-33 DEG C for 6-8 hours; bottling the pollen and storing at a low temperature; and detecting the activity of the pollen and artificially pollinating. The pitaya pollen stored by the method disclosed by the invention can keep the activity within three months and the success rate of pollinating the fresh pollen can be basically kept; and blanks in the pitaya pollen and a long-period storage period are made up, and the flowering asynchronism problem of the pitayas is successfully solved.
Description
Technical field
The present invention relates to the storage of a kind of plant corpus local, a kind of particularly method storing dragon fruit pollen.
Background technology
Dragon fruit is Cactaceae perennial climber succulent plant, and shape, like olive, can be divided into red skin red meat, red skin plain boiled pork, Calusena lansium plain boiled pork three major types by fruit pulp color, at present as dragon fruit mainly red skin red meat and red skin plain boiled pork two class of Economical cultivation.Wherein, red skin red meat dragon fruit contains a large amount of anthocyanin, has good health-care effect.But, red meat dragon fruit exists from flower not affinity phenomenon, dragon fruit pistil stigma is generally higher than stamen, when insect numbers is few or make insect activity be obstructed when wet weather, low temperature, strong wind etc., wind-force and insect to be difficult to pollen transmission, on column cap, have a strong impact on blooming of dragon fruit under field conditions (factors) and beared fruit.Poor pollination often causes the underproduction and affects quality, causes serious economic loss to orchard worker, therefore, need carry out artificial supplementary pollination to ensure yield and quality.
Although the artificial pollination Technical comparing taked on producing at present is ripe; also very practical, economical for the plantation of small-scale dragon fruit; but the effective pollination time of dragon fruit Post flowering is short; and dragon fruit flowering time be substantially at night 20:00 to 8:00 next day, dragon fruit bloom 15 ~ 20 days a collection of, bloom at every turn and last 3 ~ 5 days; for larger manufacturing enterprise; employ a lot of skilled pollination work to be unpractical in a short time, and along with price of labour power rise, dragon fruit cultivation cost increase.At night, work can cause great physical challenges to operating personnel undoubtedly, and at present, the report about the long term storage of dragon fruit pollen is still rare.Therefore, the storage of dragon fruit pollen solution hybrid strain is not met and artificial pollination, germ plasm resource storage etc. significant, most important link in dragon fruit plantation, it is key problem, only when pollen is many, pollen collecting is got up to preserve for subsequent use, the Yield and quality of guarantee dragon fruit.
In addition, the object that pollen stores research is also to keep Pollen Activity under the prerequisite not destroying germ plasm resource, extend its fertility, and the Time and place restriction overcome with this in crossbreeding, improve breeding efficiency, for interlocal kind of mass transter is provided convenience, the propagation of damage by disease and insect can be avoided simultaneously.
Summary of the invention
For solving because the reasons such as natural environment is bad cause dragon fruit to pollinate difficulty, improve the fruit-setting rate of dragon fruit and single fruit weight and fruit quality, the invention provides a kind of method solving dragon fruit flowering asynchronism, the method compensate in dragon fruit pollen, the blank of method of long term storage, successfully solves the problem of dragon fruit flowering asynchronism.
For achieving the above object, the present invention by the following technical solutions: a kind of method solving dragon fruit flowering asynchronism, comprises pollen and store and artificial pollination.
One, pollen stores:
1, gather pollen: before evening 21:00 to the next morning 7:00, with sterile writing brush or hairbrush by the pollen collecting in Pitaya Flower in sterile plastic cup, the pollen gathered 40 ~ 60 object sieves removal impurity.
2, dry: deimpurity pollen will be gone evenly to tile on pallet, thickness is 0.5 ~ 0.7cm, put into drying box, baking temperature regulates and controls to 28 ~ 33 DEG C, dry 6 ~ 8h, stirred once every 2 hours in this process, make it be heated evenly, when pollen water content is 45% ~ 55%, adds a small amount of discolour silica gel and admix in pollen, to detect the water content of pollen, in 5 minutes, namely nondiscolouring can enter next step storage work; Also can see that whether pollen is fluffy with have gentle hands is soft, be advisable on hand not stick to.
3, to bottle low-temperature storage: be (7 ~ 8) in mass ratio by pollen and discolour silica gel: 1 mixes, and reinstalls in bottle, installs rear bottle sealing one deck preservative film, adds bottle cap to cover tightly reality, puts into refrigerator, and by refrigerator temperature regulation and control to 0 ~ 5 DEG C.
4, pollen activity is detected: examine under a microscope, pollen grain is full, without broken, according to full appearance degree and the small and weak amount of breakage, can count percentage, above method can keep the activity of dragon fruit pollen 88% ~ 92%.
Preferably, drying adopts electric heating air-blowing drier.
Can dryer be put into, temperature adjusting to 90 DEG C with the discolour silica gel crossed, after 4 hours, can recover blue, can Reusability.
Two, artificial pollination:
A, in 35 ~ 40 DEG C of seasons of high temperature, at fine day Dian Shiquan in afternoon 4 ~ 6 garden sprinkling irrigation water, to improve air humidity and to reduce temperature, strengthen Pollination Effect.
The pollen of storage after the flowers are in blossom, takes out by B, dragon fruit from refrigerator, is placed in 0.5h that brown paper thaws at normal temperatures, removes discolour silica gels with 40 ~ 60 object sieves.
C, the artificial point of employing award method pollination, and being about to the pollen after thawing fully mixes, and dips in pollen point award column cap with sterile writing brush, makes pollen uniform application at post heart place, and pollination success rate reaches 85% ~ 90%.
the present invention has the following advantages:1. the dragon fruit pollen that the inventive method stores can ensure three months non-inactivations, and the success rate that can substantially keep fresh pollen to pollinate, and compensate in dragon fruit pollen, the blank of method of long term storage, successfully solves the problem of dragon fruit flowering asynchronism.
2. the amount that uses far above prior art of the water content of storage method dragon fruit pollen of the present invention, this is determined by dragon fruit pollen self-characteristic, the activity of 88 ~ 92% still can be kept after adopting method of the present invention that dragon fruit pollen can be made to be store three months in environment at 0 ~ 5 DEG C, and dragon fruit pollen is down to lower water content preserves again, it will be made to lose activity.
3. when the present invention is thawed, pollen is placed on brown paper, by the material feature of brown paper, pollen is evenly risen again, and can not adhere on medium in the process of rising again.
4. current most pollen stores is all adopt liquid nitrogen storage or ultra-low temperature storage, store in liquid nitrogen the problem of depositing precooling before storage and thawing before using, larger on pollen germination percentage impact in these processes, the present invention adopts the low-temperature storage of 0 ~ 5 DEG C to avoid above problem, achieves good storage effect.
Embodiment
embodiment 1: a kind of method solving dragon fruit flowering asynchronism, comprises pollen and stores and artificial pollination.
One, pollen stores:
1, gather pollen: in room, No. one, No. 3 ground before evening 21:00 to the next morning 7:00, with sterile writing brush or hairbrush by the pollen collecting in Pitaya Flower in sterile plastic cup, the pollen gathered 40 ~ 60 object sieves removal impurity.
2, dry: deimpurity pollen will be gone evenly to tile on pallet, thickness is 0.5cm, put into drying box, baking temperature regulates and controls to 31 DEG C, dry 6h, stirred once every 2 hours in this process, make it be heated evenly, when pollen water content is 45% ~ 55%, adds a small amount of discolour silica gel and admix in pollen, to detect the water content of pollen, in 5 minutes, namely nondiscolouring can enter next step storage work.
3, to bottle low-temperature storage: by pollen and discolour silica gel in mass ratio for 8:1 mixes, reinstall in bottle, install rear bottle sealing one deck preservative film, add bottle cap and cover tightly reality, put into refrigerator, and refrigerator temperature is regulated and controled to 0 ~ 5 DEG C.
4, pollen activity is detected: examine under a microscope, pollen grain is full, without broken, according to full appearance degree and the small and weak amount of breakage, can count percentage, above method can keep the activity of dragon fruit pollen 88% ~ 92%.
Two, artificial pollination:
A, in 35 ~ 40 DEG C of seasons of high temperature, at fine day Dian Shiquan in afternoon 4 ~ 6 garden sprinkling irrigation water, to improve air humidity and to reduce temperature, strengthen Pollination Effect.
The pollen of storage after the flowers are in blossom, takes out by B, dragon fruit from refrigerator, is placed in 0.5h that brown paper thaws at normal temperatures, removes discolour silica gels with 40 ~ 60 object sieves.
C, the artificial point of employing award method pollination, and being about to the pollen after thawing fully mixes, and dips in pollen point award column cap with sterile writing brush, makes pollen uniform application at post heart place, and pollination success rate reaches 90%.
embodiment 2: a kind of method solving dragon fruit flowering asynchronism, comprises pollen and stores and artificial pollination.
One, pollen stores:
1, gather pollen: room, No. 8 No. 3rd, ground before evening 21:00 to the next morning 7:00, with sterile writing brush or hairbrush by the pollen collecting in Pitaya Flower in sterile plastic cup, the pollen gathered 40 ~ 60 object sieves removal impurity.
2, dry: deimpurity pollen will be gone evenly to tile on pallet, thickness is 0.6cm, put into electric heating air-blowing drier, baking temperature regulates and controls to 29 DEG C, dry 8h, stirred once every 2 hours in this process, make it be heated evenly, when pollen water content is 45% ~ 55%, adds a small amount of discolour silica gel and admix in pollen, to detect the water content of pollen, in 5 minutes, namely nondiscolouring can enter next step storage work; And see that whether pollen is fluffy with have gentle hands is soft, be advisable on hand not stick to.
3, to bottle low-temperature storage: by pollen and discolour silica gel in mass ratio for 7:1 mixes, reinstall in bottle, install rear bottle sealing one deck preservative film, add bottle cap and cover tightly reality, put into refrigerator, and refrigerator temperature is regulated and controled to 0 ~ 5 DEG C.
4, pollen activity is detected: examine under a microscope, pollen grain is full, without broken, according to full appearance degree and the small and weak amount of breakage, can count percentage, above method can keep the activity of dragon fruit pollen 88% ~ 92%.
Two, artificial pollination:
A, in 35 ~ 40 DEG C of seasons of high temperature, at fine day Dian Shiquan in afternoon 4 ~ 6 garden sprinkling irrigation water, to improve air humidity and to reduce temperature, strengthen Pollination Effect.
The pollen of storage after the flowers are in blossom, takes out by B, dragon fruit from refrigerator, is placed in 0.5h that brown paper thaws at normal temperatures, removes discolour silica gels with 40 ~ 60 object sieves.
C, the artificial point of employing award method pollination, and being about to the pollen after thawing fully mixes, and dips in pollen point award column cap with sterile writing brush, makes pollen uniform application at post heart place, and pollination success rate reaches 87%.
embodiment 3: a kind of method solving dragon fruit flowering asynchronism, comprises pollen and stores and artificial pollination.
One, pollen stores:
1, gather pollen: room, No. 3 No. 2nd, ground before evening 21:00 to the next morning 7:00, with sterile writing brush or hairbrush by the pollen collecting in Pitaya Flower in sterile plastic cup, the pollen gathered 40 ~ 60 object sieves removal impurity.
2, dry: deimpurity pollen will be gone evenly to tile on pallet, thickness is 0.5 ~ 0.7cm, put into electric heating air-blowing drier, baking temperature regulates and controls to 28 DEG C, dry 7h, stirred once every 2 hours in this process, make it be heated evenly, when pollen water content is 45% ~ 55%, adds a small amount of discolour silica gel and admix in pollen, to detect the water content of pollen, in 5 minutes, namely nondiscolouring can enter next step storage work; And see that whether pollen is fluffy with have gentle hands is soft, be advisable on hand not stick to.
3, to bottle low-temperature storage: by pollen and discolour silica gel in mass ratio for 8:1 mixes, reinstall in bottle, install rear bottle sealing one deck preservative film, add bottle cap and cover tightly reality, put into refrigerator, and refrigerator temperature is regulated and controled to 0 ~ 5 DEG C.
4, pollen activity is detected: examine under a microscope, pollen grain is full, without broken, according to full appearance degree and the small and weak amount of breakage, can count percentage, above method can keep the activity of dragon fruit pollen 88% ~ 92%.
Put into dryer, temperature adjusting to 90 DEG C with the discolour silica gel crossed, after 4 hours, can recover blue, can Reusability.
Two, artificial pollination:
A, in 35 ~ 40 DEG C of seasons of high temperature, at fine day Dian Shiquan in afternoon 4 ~ 6 garden sprinkling irrigation water, to improve air humidity and to reduce temperature, strengthen Pollination Effect.
The pollen of storage after the flowers are in blossom, takes out by B, dragon fruit from refrigerator, is placed in 0.5h that brown paper thaws at normal temperatures, removes discolour silica gels with 40 ~ 60 object sieves.
C, the artificial point of employing award method pollination, and being about to the pollen after thawing fully mixes, and dips in pollen point award column cap with sterile writing brush, makes pollen uniform application at post heart place, and pollination success rate reaches 88%.
embodiment 4: a kind of method solving dragon fruit flowering asynchronism, comprises pollen and stores and artificial pollination.
One, pollen stores:
1, gather pollen: before evening 21:00 to the next morning 7:00, with sterile writing brush or hairbrush by the pollen collecting in Pitaya Flower in sterile plastic cup, the pollen gathered 40 ~ 60 object sieves removal impurity.
2, dry: deimpurity pollen will be gone evenly to tile on pallet, thickness is 0.5 ~ 0.7cm, put into drying box, baking temperature regulates and controls to 30 DEG C, dry 8h, stirred once every 2 hours in this process, make it be heated evenly, when pollen water content is 45% ~ 55%, adds a small amount of discolour silica gel and admix in pollen, to detect the water content of pollen, in 5 minutes, namely nondiscolouring can enter next step storage work; And see that whether pollen is fluffy with have gentle hands is soft, be advisable on hand not stick to.
3, to bottle low-temperature storage: by pollen and discolour silica gel in mass ratio for 7:1 mixes, reinstall in bottle, install rear bottle sealing one deck preservative film, add bottle cap and cover tightly reality, put into refrigerator, and refrigerator temperature is regulated and controled to 0 ~ 5 DEG C.
4, pollen activity is detected: examine under a microscope, pollen grain is full, without broken, according to full appearance degree and the small and weak amount of breakage, can count percentage, above method can keep the activity of dragon fruit pollen 88% ~ 92%.
Two, artificial pollination:
A, in 35 ~ 40 DEG C of seasons of high temperature, at fine day Dian Shiquan in afternoon 4 ~ 6 garden sprinkling irrigation water, to improve air humidity and to reduce temperature, strengthen Pollination Effect.
The pollen of storage after the flowers are in blossom, takes out by B, dragon fruit from refrigerator, is placed in 0.5h that brown paper thaws at normal temperatures, removes discolour silica gels with 40 ~ 60 object sieves.
C, the artificial point of employing award method pollination, and being about to the pollen after thawing fully mixes, and dips in pollen point award column cap with sterile writing brush, makes pollen uniform application at post heart place, and pollination success rate reaches 85%.
Success rate of pollinating after the different maturity periods of directly pollinating after pollen collecting in each embodiment above and store contrasts:
Note: depositing front pollination is with a collection of flower with what deposit rear pollination, and the storage time is different, not to wait to several months according to the flowers are in blossom a couple of days time and takes out pollination at any time.
Claims (4)
1. solve a method for dragon fruit flowering asynchronism, it is characterized in that, comprise pollen and store and artificial pollination,
One, pollen stores:
1, gather pollen: before evening 21:00 to the next morning 7:00, with sterile writing brush or hairbrush by the pollen collecting in Pitaya Flower in sterile plastic cup, the pollen gathered 40 ~ 60 object sieves removal impurity;
2, dry: deimpurity pollen will be gone evenly to tile on pallet, thickness is 0.5 ~ 0.7cm, put into drying box, baking temperature regulates and controls to 28 ~ 33 DEG C, dry 6 ~ 8h, stirred once every 2 hours in this process, make it be heated evenly, when pollen water content is 45% ~ 55%, adds a small amount of discolour silica gel and admix in pollen, to detect the water content of pollen, in 5 minutes, namely nondiscolouring can enter next step storage work;
3, to bottle low-temperature storage: be (7 ~ 8) in mass ratio by pollen and discolour silica gel: 1 mixes, and reinstalls in bottle, installs rear bottle sealing one deck preservative film, adds bottle cap to cover tightly reality, puts into refrigerator, and by refrigerator temperature regulation and control to 0 ~ 5 DEG C;
4, pollen activity is detected: examine under a microscope, pollen grain is full, without broken, according to full appearance degree and the small and weak amount of breakage, can count percentage, above method can keep the activity of dragon fruit pollen 88% ~ 92%;
Two, artificial pollination:
A, in 35 ~ 40 DEG C of seasons of high temperature, at fine day Dian Shiquan in afternoon 4 ~ 6 garden sprinkling irrigation water, to improve air humidity and to reduce temperature, strengthen Pollination Effect;
The pollen of storage after the flowers are in blossom, takes out by B, dragon fruit from refrigerator, is placed in 0.5h that brown paper thaws at normal temperatures, removes discolour silica gels with 40 ~ 60 object sieves;
C, the artificial point of employing award method pollination, and being about to the pollen after thawing fully mixes, and dips in pollen point award column cap with sterile writing brush, makes pollen uniform application at post heart place, and pollination success rate reaches 85% ~ 90%.
2. a kind of method solving dragon fruit flowering asynchronism according to claim 1, is characterized in that, dry employing electric heating air-blowing drier.
3. a kind of method solving dragon fruit flowering asynchronism according to claim 1 and 2, is characterized in that, when detecting the water content of pollen in drying steps, sees that whether pollen is fluffy, be advisable on hand not stick to have gentle hands is soft.
4. a kind of method solving dragon fruit flowering asynchronism according to claim 1 and 2, is characterized in that, puts into dryer, temperature adjusting to 90 DEG C by with the discolour silica gel crossed, and can recover blue after 4 hours, can Reusability.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510527021.3A CN105104172A (en) | 2015-08-26 | 2015-08-26 | Method for solving flowering asynchronism of pitayas |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510527021.3A CN105104172A (en) | 2015-08-26 | 2015-08-26 | Method for solving flowering asynchronism of pitayas |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105104172A true CN105104172A (en) | 2015-12-02 |
Family
ID=54652518
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510527021.3A Pending CN105104172A (en) | 2015-08-26 | 2015-08-26 | Method for solving flowering asynchronism of pitayas |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105104172A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105494081A (en) * | 2015-12-09 | 2016-04-20 | 防城港市星湖生态农业有限公司 | Pollination method for pitaya |
| CN105961376A (en) * | 2016-05-31 | 2016-09-28 | 陆川县大桥镇家君种植场 | Storage method for keeping pitaya pollen activity |
| CN112205392A (en) * | 2020-10-12 | 2021-01-12 | 潍坊郭牌农业科技有限公司 | Watermelon pollen storage and preservation process |
| CN115790094A (en) * | 2023-01-28 | 2023-03-14 | 海南浙江大学研究院 | Pollen drying device and pollen drying method |
| CN117441609A (en) * | 2023-12-20 | 2024-01-26 | 浙江大学海南研究院 | Preparation method and application of pollen mixture |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102362578A (en) * | 2011-06-10 | 2012-02-29 | 湖北省烟草科研所 | Electrothermal air-blast constant-temperature drying collecting method of tobacco pollens |
| CN103719076A (en) * | 2013-12-25 | 2014-04-16 | 广东省农业科学院果树研究所 | Litchi pollen collecting and storing method |
-
2015
- 2015-08-26 CN CN201510527021.3A patent/CN105104172A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102362578A (en) * | 2011-06-10 | 2012-02-29 | 湖北省烟草科研所 | Electrothermal air-blast constant-temperature drying collecting method of tobacco pollens |
| CN103719076A (en) * | 2013-12-25 | 2014-04-16 | 广东省农业科学院果树研究所 | Litchi pollen collecting and storing method |
Non-Patent Citations (3)
| Title |
|---|
| CHRISTIANE METZ等: "Viability of Pollen of Two Fruit Crop Cacti of the Genus Hylocereus Is Affected by Temperature and Duration of Storage", 《HORTSCIENCE》 * |
| 张殿高: "《果树栽培实用技术》", 30 April 1990, 辽宁科学技术出版社 * |
| 韦茜等: "火龙果授粉技术", 《福建果树》 * |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105494081A (en) * | 2015-12-09 | 2016-04-20 | 防城港市星湖生态农业有限公司 | Pollination method for pitaya |
| CN105961376A (en) * | 2016-05-31 | 2016-09-28 | 陆川县大桥镇家君种植场 | Storage method for keeping pitaya pollen activity |
| CN112205392A (en) * | 2020-10-12 | 2021-01-12 | 潍坊郭牌农业科技有限公司 | Watermelon pollen storage and preservation process |
| CN112205392B (en) * | 2020-10-12 | 2022-03-15 | 潍坊郭牌农业科技有限公司 | Watermelon pollen storage and preservation process |
| CN115790094A (en) * | 2023-01-28 | 2023-03-14 | 海南浙江大学研究院 | Pollen drying device and pollen drying method |
| CN115790094B (en) * | 2023-01-28 | 2023-05-26 | 海南浙江大学研究院 | Pollen drying device and pollen drying method |
| CN117441609A (en) * | 2023-12-20 | 2024-01-26 | 浙江大学海南研究院 | Preparation method and application of pollen mixture |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103493725B (en) | Fructus actinidiae chinensis artificial pollination method and Kiwi fruit pollen used prepare method | |
| CN105104172A (en) | Method for solving flowering asynchronism of pitayas | |
| CN102960212B (en) | All-branch grafting method for fruit tree | |
| CN103548652B (en) | Rapid tree cultivation method for dendrobium candidum tissue-culture bottle seedlings | |
| CN103109715B (en) | Simplified tree form reforming method suitable for high-density jujube orchard | |
| CN105684811B (en) | A kind of method of pineapple Sakyamuni teletostage fruit production | |
| CN104904458B (en) | A kind of field management method of muskmelon facility Long term cultivation | |
| CN102599063A (en) | Rapid propagation method of Bletilla striata | |
| CN110050690A (en) | Southern area sweet cherry and cherry interspecific hybridization rescue culture seedling establishment method | |
| CN105850725A (en) | Pitaya pollination method | |
| CN104855099A (en) | Method for cultivating pitaya in greenhouse | |
| CN107969237A (en) | A kind of high-quality grapes selection based on cross-pollination | |
| CN105613275A (en) | Prunus pollination method | |
| CN101653077A (en) | Method for picking tops, culturing balls, adjusting and controlling florescence of new lilium longiflorum | |
| CN104430304A (en) | Method for short-term preservation of Citrullus lanatus Mansfeld pollen | |
| CN104823673B (en) | A kind of Plant adjustment method of muskmelon Long term cultivation | |
| CN104145947A (en) | Vacuum storage method and application of soybean pollen | |
| CN103340102B (en) | Breeding method for good-quality high-yield grape variety suitable for growing in northern Hunan | |
| CN106489730B (en) | A kind of Herba Renantherae coccineae once-seedling forming quick propagating method and culture medium | |
| CN105210884A (en) | Bletilla seed asepsis sprouting equilibrated Medium | |
| CN107836349A (en) | A kind of plant stem cell cultural method | |
| CN104663426A (en) | Method for producing seeds by adopting cucumis melo facility in greenhouse | |
| CN104719156A (en) | Method for performing in-vivo induction on vaccinium australe tetraploid | |
| CN104969851A (en) | Method of improving pollination on Fuji apples | |
| CN106879453A (en) | A kind of pollinating method of greenhouse peach |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20151202 |