CN105210884A - Bletilla seed asepsis sprouting equilibrated Medium - Google Patents
Bletilla seed asepsis sprouting equilibrated Medium Download PDFInfo
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- CN105210884A CN105210884A CN201510758526.0A CN201510758526A CN105210884A CN 105210884 A CN105210884 A CN 105210884A CN 201510758526 A CN201510758526 A CN 201510758526A CN 105210884 A CN105210884 A CN 105210884A
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Abstract
The invention discloses a kind of bletilla seed asepsis sprouting equilibrated Medium.Does described medium consist of: comprise the MS basal medium of adjustment, 100-150ml/L coconut milk, 0-2mg/L? 6-BA, 0-2mg/L? the particular solid medium of NAA.Adopt bletilla seed asepsis sprouting equilibrated Medium of the present invention, cultivated by bletilla seed on equilibrated Medium, can obtain root through inoculated and cultured, pseudobulb, leaf break up complete bletilla aseptic seedling, germination rate reaches more than 90%.The present invention effectively can solve bletilla seed asepsis and cultivate the more problem of inoculation step, reduces labour intensity and reduces production cost, can be used for the large-scale production of bletilla seedling.
Description
Technical field
The invention belongs to plant tissue culture fast breeding technique field; Specifically a kind of bletilla seed asepsis sprouting equilibrated Medium.
Background technology
Bletilla (Bletillastriata) is the orchid family bletilla platymiscium, produces the ground such as Southern Shaanxi, the southeast of Gansu, Jiangsu, Anhui, Zhejiang, Jiangxi, Fujian, Hubei, Hunan, Guangdong, Guangxi, Sichuan and Guizhou.Under being born in the evergreen broad-leaved sylvan life of height above sea level 100-3200 rice, a woods or softwood forest and roadside thick grass or rock seam in.Its dry tuber is famous Chinese medicine, medication long history.Property is bitter, puckery, sweet, is slightly cold, enters to return lung, liver, stomach warp, have astringing to arrest bleeding, heat-clearing disappears wet, detumescence and promoting granulation, the effect such as antibacterial.Be widely used in treatment inside and outside clinically and go out blood trouble as hemoptysis haematemesis, traumatism and bleeding, chapped skin, pulmonary tuberculosis hemoptysis, ulcer haemorrhage; Sore swollen toxin; Burn and scald; Immunological regulation; Tumour etc., evident in efficacy.Therefore, bletilla has very high medical value and economic worth.Traditionally, bletilla medication mainly utilizes wild resource, and along with the intensification of using degree, resource is atrophy gradually, and current bletilla is listed in Chinese Second Class Key Protected Plant, and resource disparities between supply and demand are comparatively outstanding.Therefore, bletilla manual scale planting is the only way solving bletilla resources, meet current demand.Bletilla is perennial herb, although can yield positive results, seed without endosperm, in its natural state, few Germination And Seedling.Traditionally, medicinal herb grower mainly relies on stem tuber to breed, and reproductive efficiency is low, and stem tuber consumption is large, and planting cost is high.Chinese scholars is attempted group culturation rapid propagating technology and is carried out bletilla seedling breeding, but traditional tissue culture technology existence group training inoculation link is many, high in cost of production drawback.Therefore, how to optimize bletilla group culturation rapid propagating technology, reduce seeling industry cost, there is larger realistic meaning and value.
Summary of the invention
The object of the invention is to provide a kind of bletilla seed asepsis sprouting equilibrated Medium for the deficiencies in the prior art, adopt this medium that seed inoculated and cultured acquisition root, pseudobulb, a leaf can be utilized to break up complete bletilla aseptic seedling, to solve bletilla seed not containing endosperm, be difficult under nature sprout or germination rate extremely low, cultivate conventionally by seed asepsis and produce bletilla aseptic seedling, inoculation step is more, the problem that production cost is high.
The technical solution used in the present invention: bletilla seed asepsis sprouting equilibrated Medium, described medium consists of: the particular solid medium comprising the MS basal medium of adjustment, 100-150ml/L coconut milk, 0-2mg/L6-BA, 0-2mg/LNAA.
Adopt bletilla seed asepsis sprouting equilibrated Medium to carry out bletilla seed asepsis and cultivate the method sprouted, comprise the following steps:
(1) the bletilla capsule running water of collection, 84 thimerosals, 75% alcohol, sterile distilled water are carried out combined sterilizing sterilizing.
(2) by bletilla planting seed on aseptic culture medium.Medium consists of: the particular solid medium comprising the MS basal medium of adjustment, 100-150ml/L coconut milk, 0-2mg/L6-BA, 0-2mg/LNAA.
(3) by broadcast seed-bearing blake bottle and be placed in the intensity of illumination of 1600-2000LX, room temperature 20-30 DEG C environmental condition under, cultivate 4-5 month, obtain root, pseudobulb, leaf break up complete bletilla tissue-culture container seedling.
(4) bottle seedling is transferred in cultivation matrix carry out hardening cultivation; Controlling daytime growth temperature is 15 25 ° of C, and relative air humidity remains on more than 70%, with covered rearing with plastic film above outdoor hardening, and arranges sunshade net and suitably shelters from heat or light;
(5) healthy and strong seedling is obtained.
Adopt bletilla seed asepsis sprouting equilibrated Medium of the present invention, cultivated by bletilla seed on equilibrated Medium, can obtain root through inoculated and cultured, pseudobulb, leaf break up complete bletilla aseptic seedling, germination rate reaches more than 90%.The present invention efficiently solves bletilla seed asepsis and cultivates the more problem of inoculation step, reduces labour intensity and reduces production cost, can be used for the large-scale production of bletilla seedling.
Embodiment
Embodiment 1
(1) in gathering ripe uncracked bletilla capsule by the end of August, with tap water several; 84 thimerosals sterilize 30 minutes, 75% alcohol disinfecting 10 minutes, sterile distilled water wash 3 times, carries out combined sterilizing sterilizing.
(2) by bletilla planting seed on aseptic culture medium.Medium consists of: the particular solid medium comprising the MS basal medium of adjustment, 150ml/L coconut milk, 0mg/L6-BA, 0mg/LNAA;
(3) will broadcast under seed-bearing blake bottle is placed in intensity of illumination 1500-2000LX, temperature 20-30 DEG C environmental condition, and cultivate 4-5 month, and obtain root, pseudobulb, leaf break up complete bletilla tissue-culture container seedling, germination rate reaches 90%;
(4) seedling is transferred in cultivation matrix carry out hardening cultivation; Controlling daytime growth temperature is 15 25 DEG C, and relative air humidity remains on more than 70%, top covered rearing with plastic film, and arranges sunshade net and suitably shelter from heat or light;
(5) healthy and strong seedling is obtained.
Embodiment 2
(1) in gathering ripe uncracked bletilla capsule by the end of August, with tap water several; 84 thimerosals sterilize 30 minutes, 75% alcohol disinfecting 10 minutes, sterile distilled water wash 3 times, carries out combined sterilizing sterilizing.
(2) by bletilla planting seed on aseptic culture medium.Medium consists of: the particular solid medium comprising the MS basal medium of adjustment, 150ml/L coconut milk, 0.05mg/L6-BA, 0.1mg/LNAA;
(3) will broadcast under seed-bearing blake bottle is placed in intensity of illumination 1500-2000LX, temperature 20-30 DEG C environmental condition, and cultivate 4-5 month, and obtain root, pseudobulb, leaf break up complete bletilla tissue-culture container seedling, germination rate reaches 90%;
(4) seedling is transferred in cultivation matrix carry out hardening cultivation; Controlling daytime growth temperature is 15 25 DEG C, and relative air humidity remains on more than 70%, top covered rearing with plastic film, and arranges sunshade net and suitably shelter from heat or light;
(5) healthy and strong seedling is obtained.
Claims (2)
1. bletilla seed asepsis sprouting equilibrated Medium, is characterized in that: described medium consists of: the particular solid medium comprising the MS basal medium of adjustment, 100-150ml/L coconut milk, 0-2mg/L6-BA, 0-2mg/LNAA.
2. adopt bletilla seed asepsis sprouting equilibrated Medium to carry out bletilla seed asepsis and cultivate the method sprouted, it is characterized in that: comprise the following steps:
(1) the bletilla capsule running water of collection, 84 thimerosals, 75% alcohol, sterile distilled water are carried out combined sterilizing sterilizing;
(2) by bletilla planting seed on aseptic culture medium, described medium consists of: the particular solid medium comprising the MS basal medium of adjustment, 100-150ml/L coconut milk, 0-2mg/L6-BA, 0-2mg/LNAA;
(3) by broadcast seed-bearing blake bottle and be placed in the intensity of illumination of 1600-2000LX, room temperature 20-30 DEG C environmental condition under, cultivate 4-5 month, obtain root, pseudobulb, leaf break up complete bletilla tissue-culture container seedling;
(4) bottle seedling is transferred in cultivation matrix carry out hardening cultivation; Controlling daytime growth temperature is 15 25 ° of C, and relative air humidity remains on more than 70%, with covered rearing with plastic film above outdoor hardening, and arranges sunshade net and suitably shelters from heat or light;
(5) healthy and strong seedling is obtained.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201510758526.0A CN105210884A (en) | 2015-11-10 | 2015-11-10 | Bletilla seed asepsis sprouting equilibrated Medium |
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| CN201510758526.0A CN105210884A (en) | 2015-11-10 | 2015-11-10 | Bletilla seed asepsis sprouting equilibrated Medium |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106508406A (en) * | 2016-11-24 | 2017-03-22 | 贵州盛茂白芨开发有限公司 | Tissue culture and rapid propagation method of bletilla striata seedlings |
| CN107046851A (en) * | 2017-04-12 | 2017-08-18 | 西南林业大学 | Bletilla greenhouse direct sowing and seedling method by material of seed |
| CN111903530A (en) * | 2020-09-23 | 2020-11-10 | 宣威市福康生物科技有限公司 | Tissue culture seedling method for bletilla striata |
| CN114431110A (en) * | 2022-02-07 | 2022-05-06 | 中国科学院武汉植物园 | Bletilla striata seed culture medium combination and method for culturing bletilla striata seeds by using bletilla striata seed culture medium combination |
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| CN102907323A (en) * | 2012-11-01 | 2013-02-06 | 建始县颐泰生物科技有限公司 | Method for aseptically producing miniature seed stems of common bletilla pseudobulb seeds |
| CN102972290A (en) * | 2012-11-15 | 2013-03-20 | 贵州师范大学 | Method for improving transplanting survival rate of rhizoma bletillae tissue culture seedling |
| CN104145816A (en) * | 2014-07-29 | 2014-11-19 | 郎溪县紫兰药材种植专业合作社 | Bletilla striata tissue-culture seedling raising method |
| CN104335903A (en) * | 2014-11-21 | 2015-02-11 | 广西中医药大学 | Method for accelerating quick propagation of rhizoma bletillae |
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| CN102972290A (en) * | 2012-11-15 | 2013-03-20 | 贵州师范大学 | Method for improving transplanting survival rate of rhizoma bletillae tissue culture seedling |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106508406A (en) * | 2016-11-24 | 2017-03-22 | 贵州盛茂白芨开发有限公司 | Tissue culture and rapid propagation method of bletilla striata seedlings |
| CN106508406B (en) * | 2016-11-24 | 2019-07-12 | 贵州盛茂白芨开发有限公司 | Bletilla seedling tissue culture and rapid propagation method |
| CN107046851A (en) * | 2017-04-12 | 2017-08-18 | 西南林业大学 | Bletilla greenhouse direct sowing and seedling method by material of seed |
| CN107046851B (en) * | 2017-04-12 | 2020-07-10 | 西南林业大学 | Bletilla striata greenhouse direct seeding seedling method taking seeds as materials |
| CN111903530A (en) * | 2020-09-23 | 2020-11-10 | 宣威市福康生物科技有限公司 | Tissue culture seedling method for bletilla striata |
| CN114431110A (en) * | 2022-02-07 | 2022-05-06 | 中国科学院武汉植物园 | Bletilla striata seed culture medium combination and method for culturing bletilla striata seeds by using bletilla striata seed culture medium combination |
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