CN103098713B - Dendrobium officinale domesticated breeding cultivation method - Google Patents
Dendrobium officinale domesticated breeding cultivation method Download PDFInfo
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- CN103098713B CN103098713B CN201310058427.2A CN201310058427A CN103098713B CN 103098713 B CN103098713 B CN 103098713B CN 201310058427 A CN201310058427 A CN 201310058427A CN 103098713 B CN103098713 B CN 103098713B
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- 238000012364 cultivation method Methods 0.000 title abstract description 5
- 241001076416 Dendrobium tosaense Species 0.000 title abstract 4
- 238000009395 breeding Methods 0.000 title abstract 3
- 230000001488 breeding effect Effects 0.000 title abstract 3
- 238000000034 method Methods 0.000 claims abstract description 14
- 230000004069 differentiation Effects 0.000 claims abstract description 7
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 6
- 230000006698 induction Effects 0.000 claims abstract description 6
- 241000026010 Dendrobium candidum Species 0.000 claims description 23
- 238000005286 illumination Methods 0.000 claims description 18
- 241000234295 Musa Species 0.000 claims description 15
- 235000018290 Musa x paradisiaca Nutrition 0.000 claims description 15
- 239000006870 ms-medium Substances 0.000 claims description 15
- 239000006160 differential media Substances 0.000 claims description 10
- 230000001939 inductive effect Effects 0.000 claims description 10
- 235000013575 mashed potatoes Nutrition 0.000 claims description 5
- 238000004659 sterilization and disinfection Methods 0.000 claims description 5
- 241000196324 Embryophyta Species 0.000 abstract description 4
- 230000004083 survival effect Effects 0.000 abstract description 3
- 230000000249 desinfective effect Effects 0.000 abstract 1
- 239000001963 growth medium Substances 0.000 abstract 1
- 240000004638 Dendrobium nobile Species 0.000 description 3
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 238000011534 incubation Methods 0.000 description 2
- 239000002609 medium Substances 0.000 description 2
- 235000015961 tonic Nutrition 0.000 description 2
- 230000001256 tonic effect Effects 0.000 description 2
- 244000025254 Cannabis sativa Species 0.000 description 1
- 241000233855 Orchidaceae Species 0.000 description 1
- 240000001519 Verbena officinalis Species 0.000 description 1
- 235000018718 Verbena officinalis Nutrition 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 210000002615 epidermis Anatomy 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 230000003014 reinforcing effect Effects 0.000 description 1
- 230000001850 reproductive effect Effects 0.000 description 1
- 239000011435 rock Substances 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 229960000716 tonics Drugs 0.000 description 1
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- Cultivation Of Plants (AREA)
Abstract
A Dendrobium officinale domesticated breeding cultivation method belongs to plant cultivation method. The method comprises the following steps: 1) disinfecting seeds produced by domesticated Dendrobium officinale with alcohol; 2) conducting induction culture on protocorm; 3) conducting differentiation culture; 4) conducting rooting culture; and 5) conducting hardening-seedling treatment, and planting in a greenhouse. The Dendrobium officinale domesticated breeding cultivation method has reasonable design; different culture media and culture conditions are employed for culture in different stages, so as to realize a final average rooting rate of 98% and an average transplanting survival rate of 97%, shorten culture cycle, and simplify culture steps.
Description
Technical field
The invention belongs to plant cultivation method, be specifically related to dendrobium candidum domestication reproducing and cultivating method.
Background technology
Dendrobium candidum, herbaceous plant grows nonparasitically upon another plant for the orchid family is perennial.Be born in height above sea level and reach on the rock that the mountain region half of 1600 meters is dark and damp, the environment of happiness warm and moist weather and half cloudy half light, cold-resistant.The low temperature of general equal ability-5 ℃.The stem of noble dendrobium can be divided into tens of kinds, yellow grass, Jin Chai, horsewhip etc., the superfine product that dendrobium candidum is the stem of noble dendrobium, and because epidermis is, iron is green gains the name for it.Dendrobium candidum has unique medical value, with its stem, be used as medicine, Chinese medicine name: the stem of noble dendrobium, belongs to the yin tonics in tonic, < < Chinese pharmacopoeia > >: reinforcing stomach reg fluid is nourishing Yin and clearing heat.
Existing dendrobium candidum cultural method is a lot, but its ubiquity reproductive efficiency is low, and differentiation is slow, is difficult to carry out the problems such as industrialized propagation.
Summary of the invention
The problem existing for prior art, the object of the invention is to design provides the technical scheme of dendrobium candidum domestication reproducing and cultivating method.
Described dendrobium candidum domestication reproducing and cultivating method, is characterized in that comprising following processing step:
1) learn from else's experience domestication dendrobium candidum produce seed, use alcohol disinfection;
2) seed broadcasting step 1) being obtained is cultivated in protocorm inducing culture, described protocorm inducing culture is MS medium+7～9% banana puree, condition of culture is: 21～23 ℃ of temperature, humidity 35～40%, illumination condition is 2000～2500LUX, light application time 12h/d, cultivates 1～2 month;
3) protocorm being put into differential medium cultivates, described differential medium is MS medium+7～9% banana puree+10～12% mashed potatoes+NAA0.5mg/L, condition of culture is: 24～28 ℃ of temperature, humidity 40～50%, illumination condition is 1500～2000LUX, light application time 12h/d, cultivates 15～20 days;
4) the clump bud cutting of induction differentiation is divided into simple bud, then putting into root media cultivates, described root media is MS medium+7～9% banana puree+IBA0.5mg/L+ NAA0.25mg/L, condition of culture is: 24～28 ℃ of temperature, humidity 40～50%, illumination condition is 1500～2000LUX, and light application time 12h/d cultivates 20～35 days;
5) seedling step 4) being obtained carries out in hardening processing, and then carries out booth field planting.
Described dendrobium candidum domestication reproducing and cultivating method, is characterized in that in described step 1) that Seeds of Dendrobium Candidum kind age is 4～6 months.
Described dendrobium candidum domestication reproducing and cultivating method, is characterized in that described step 2) in condition of culture be: 21.5～22.5 ℃ of temperature, humidity 37～40%, illumination condition is 2100～2400LUX, light application time 12h/d.
Described dendrobium candidum domestication reproducing and cultivating method, is characterized in that in described step 3), condition of culture is: 25～27 ℃ of temperature, and humidity 45～50%, illumination condition is 1500～1800LUX, light application time 12h/d.
Described dendrobium candidum domestication reproducing and cultivating method, is characterized in that in described step 4), condition of culture is: 25～27 ℃ of temperature, and humidity 45～50%, illumination condition is 1500～1800LUX, light application time 12h/d.
Above-mentioned a kind of dendrobium candidum domestication reproducing and cultivating method, reasonable in design, in different phase, adopt different medium and condition of culture to cultivate, finally reaching average rooting rate is 98%, average transplanting survival rate is 97%, and has greatly shortened cultivation cycle, has simplified incubation step.
Embodiment
Below in conjunction with embodiment, further illustrate the present invention.The percentage relating in the present invention is all weight percentage.
Embodiment 1
1) seed that kind age that the dendrobium candidum of domestication produces is 4 months of learning from else's experience, uses alcohol disinfection;
2) seed broadcasting step 1) being obtained is cultivated in protocorm inducing culture, and described protocorm inducing culture is MS medium+8% banana puree, and condition of culture is: 22 ℃ of temperature, humidity 38%, illumination condition is 2200LUX, and light application time 12h/d cultivates 1 month;
3) protocorm is put into differential medium and cultivate, described differential medium is MS medium+8% banana puree+10% mashed potatoes+NAA0.5mg/L, and condition of culture is: 26 ℃ of temperature, humidity 45%, illumination condition is 1800LUX, and light application time 12h/d cultivates 18 days;
4) the clump bud cutting of induction differentiation is divided into simple bud, then putting into root media cultivates, described root media is MS medium+8% banana puree+IBA0.5mg/L+ NAA0.25mg/L, condition of culture is: 26 ℃ of temperature, humidity 45%, illumination condition is 1800LUX, and light application time 12h/d cultivates 25 days;
5) seedling step 4) being obtained carries out in hardening processing, and then carries out booth field planting.
Embodiment 2
1) seed that kind age that the dendrobium candidum of domestication produces is 5 months of learning from else's experience, uses alcohol disinfection;
2) seed broadcasting step 1) being obtained is cultivated in protocorm inducing culture, and described protocorm inducing culture is MS medium+7% banana puree, and condition of culture is: 21 ℃ of temperature, humidity 40%, illumination condition is 2000LUX, and light application time 12h/d cultivates 1.5 months;
3) protocorm is put into differential medium and cultivate, described differential medium is MS medium+7% banana puree+11% mashed potatoes+NAA0.5mg/L, and condition of culture is: 24 ℃ of temperature, humidity 40%, illumination condition is 1500LUX, and light application time 12h/d cultivates 15 days;
4) the clump bud cutting of induction differentiation is divided into simple bud, then putting into root media cultivates, described root media is MS medium+7% banana puree+IBA0.5mg/L+ NAA0.25mg/L, condition of culture is: 24 ℃ of temperature, humidity 40%, illumination condition is 1500LUX, and light application time 12h/d cultivates 20 days;
5) seedling step 4) being obtained carries out in hardening processing, and then carries out booth field planting.
Embodiment 3
1) seed that kind age that the dendrobium candidum of domestication produces is 6 months of learning from else's experience, uses alcohol disinfection;
2) seed broadcasting step 1) being obtained is cultivated in protocorm inducing culture, and described protocorm inducing culture is MS medium+9% banana puree, and condition of culture is: 23 ℃ of temperature, humidity 35%, illumination condition is 2500LUX, and light application time 12h/d cultivates 2 months;
3) protocorm is put into differential medium and cultivate, described differential medium is MS medium+9% banana puree+12% mashed potatoes+NAA0.5mg/L, and condition of culture is: 28 ℃ of temperature, humidity 50%, illumination condition is 2000LUX, and light application time 12h/d cultivates 20 days;
4) the clump bud cutting of induction differentiation is divided into simple bud, then putting into root media cultivates, described root media is MS medium+9% banana puree+IBA0.5mg/L+ NAA0.25mg/L, condition of culture is: 28 ℃ of temperature, humidity 50%, illumination condition is 2000LUX, and light application time 12h/d cultivates 35 days;
5) seedling step 4) being obtained carries out in hardening processing, and then carries out booth field planting.
Above-mentioned a kind of dendrobium candidum domestication reproducing and cultivating method, reasonable in design, in different phase, adopt different medium and condition of culture to cultivate, finally reaching average rooting rate is 98%, average transplanting survival rate is 97%, and has greatly shortened cultivation cycle, has simplified incubation step.
Claims (2)
1. dendrobium candidum domestication reproducing and cultivating method, is characterized in that comprising following processing step:
1) learn from else's experience domestication dendrobium candidum produce seed, use alcohol disinfection;
2) seed broadcasting step 1) being obtained is cultivated in protocorm inducing culture, described protocorm inducing culture is MS medium+7～9% banana puree, condition of culture is: 21.5～22.5 ℃ of temperature, humidity 37～40%, illumination condition is 2100～2400LUX, light application time 12h/d, cultivates 1～2 month;
3) protocorm being put into differential medium cultivates, described differential medium is MS medium+7～9% banana puree+10～12% mashed potatoes+NAA0.5mg/L, condition of culture is: 25～27 ℃ of temperature, humidity 45～50%, illumination condition is 1500～1800LUX, light application time 12h/d, cultivates 15～20 days;
4) the clump bud cutting of induction differentiation is divided into simple bud, then putting into root media cultivates, described root media is MS medium+7～9% banana puree+IBA0.5mg/L+ NAA0.25mg/L, condition of culture is: 25～27 ℃ of temperature, humidity 45～50%, illumination condition is 1500～1800LUX, and light application time 12h/d cultivates 20～35 days;
5) seedling step 4) being obtained carries out hardening processing, and then carries out booth field planting.
2. dendrobium candidum as claimed in claim 1 domestication reproducing and cultivating method, is characterized in that in described step 1) that Seeds of Dendrobium Candidum kind age is 4～6 months.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106386498A (en) * | 2016-10-19 | 2017-02-15 | 嘉兴华圣农业生物科技有限公司 | Tissue culture rapid propagation method for Herba Dendrobii |
| CN107711517A (en) * | 2017-12-01 | 2018-02-23 | 江西宋氏灵斛生物科技有限公司 | A kind of candidum tissue culturing seedling breeding method of high-survival rate |
| CN108668893B (en) * | 2018-04-23 | 2021-08-20 | 福建省农业科学院农业生物资源研究所 | Rapid seedling raising method for spiranthes sinensis seeds |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1608424A (en) * | 2004-11-24 | 2005-04-27 | 上海增靓生物科技有限公司 | Large-area cultivation of officinal dendrobium |
| CN101213940A (en) * | 2008-01-18 | 2008-07-09 | 中国科学院昆明植物研究所 | Fast replication method for dendrobium |
| CN101213941A (en) * | 2008-01-18 | 2008-07-09 | 中国科学院昆明植物研究所 | Fast replication and in-vitro conservation method for dendrobium |
| CN101933456A (en) * | 2009-06-29 | 2011-01-05 | 西双版纳增靓生物科技有限公司 | Method for quickly breeding seedlings of dendrobium officinale capsule |
| CN102119655A (en) * | 2010-07-29 | 2011-07-13 | 云南红土生源药用生物科技开发有限公司 | Natural light rapid breeding method for dendrobium officinale |
| CN102613086A (en) * | 2012-03-31 | 2012-08-01 | 南京农业大学 | Hormone-free tissue culture method for dendrobium candidum |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| TWI369179B (en) * | 2008-01-08 | 2012-08-01 | Nat Univ Kaohsiung | A method for producing polyploid plants of orchids |
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Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1608424A (en) * | 2004-11-24 | 2005-04-27 | 上海增靓生物科技有限公司 | Large-area cultivation of officinal dendrobium |
| CN101213940A (en) * | 2008-01-18 | 2008-07-09 | 中国科学院昆明植物研究所 | Fast replication method for dendrobium |
| CN101213941A (en) * | 2008-01-18 | 2008-07-09 | 中国科学院昆明植物研究所 | Fast replication and in-vitro conservation method for dendrobium |
| CN101933456A (en) * | 2009-06-29 | 2011-01-05 | 西双版纳增靓生物科技有限公司 | Method for quickly breeding seedlings of dendrobium officinale capsule |
| CN102119655A (en) * | 2010-07-29 | 2011-07-13 | 云南红土生源药用生物科技开发有限公司 | Natural light rapid breeding method for dendrobium officinale |
| CN102613086A (en) * | 2012-03-31 | 2012-08-01 | 南京农业大学 | Hormone-free tissue culture method for dendrobium candidum |
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Address after: 311404 shimenwu, Chakou village, Dongqiao Town, Fuyang City, Hangzhou City, Zhejiang Province Patentee after: Hangzhou Fuyang Wenqu Ecological Agriculture Development Co.,Ltd. Address before: 311404 shimenwu, Chakou village, Dongqiao Town, Fuyang City, Hangzhou City, Zhejiang Province Patentee before: FUYANG WENQU ECOLOGICAL AGRICULTURE DEVELOPMENT Co.,Ltd. |
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Effective date of registration: 20240109 Address after: 311404 Dong Qiao Zhen Xian De Cun, Fuyang District, Hangzhou City, Zhejiang Province Patentee after: Hangzhou Fuyang Malaysia Tools Co.,Ltd. Address before: 311404 shimenwu, Chakou village, Dongqiao Town, Fuyang City, Hangzhou City, Zhejiang Province Patentee before: Hangzhou Fuyang Wenqu Ecological Agriculture Development Co.,Ltd. |
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Denomination of invention: Domestication and propagation cultivation methods of Dendrobium officinale Granted publication date: 20140416 Pledgee: Nanxun Zhejiang rural commercial bank Limited by Share Ltd. Fuyang branch Pledgor: Hangzhou Fuyang Malaysia Tools Co.,Ltd. Registration number: Y2024980007159 |