CN103798013B - A kind of breeding method of Seeds of Dendrobium Candidum seedling - Google Patents
A kind of breeding method of Seeds of Dendrobium Candidum seedling Download PDFInfo
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Abstract
The present invention relates to a kind of breeding method of Seeds of Dendrobium Candidum seedling, belong to the field of planting technology; Concrete steps are host materials that the sawdust be suitable for thickness is cultivated for seedlings of Dendrobium officinale, load container for plant growth, be placed in greenhouse or plastic tunnel, matrix is irrigated with clean well water or mountain spring water, smooth through appropriateness, suppression after, cultivate through human assistance self-pollination the Powdered seed collected in the dendrobium candidum capsule of 4-5 month by from prior, be seeded into seedling medium surface equably, then the nurturing an environment that regulable control is suitable, makes seed fast germination grow into supply and produces seed that plantation uses and to grow directly from seeds seedling; This method adopts artificial breeding facilities to breed dendrobium candidum to grow directly from seeds seedling, and seedling raising environment, close to its plant development environment, has strong stress resistance, robust growth, the features such as damage by disease and insect is few, superior product quality with the seedling that the method is cultivated.
Description
Technical field
The present invention relates to a kind of breeding method of Seeds of Dendrobium Candidum seedling, be specifically related to a kind of method utilizing dendrobium candidum capsule cultivating seeds seedlings of Dendrobium officinale, belong to the field of planting technology.
Background technology
The stem of noble dendrobium is the high-grade traditional Chinese medicine of the large quasi-tradition of China one, also be famous and precious medicinal and edible plant, both can be used for medicinal purpose traditionally, health products can be used as again, simultaneously, start from way back also to be used by as the high-grade soup stock of one (food) till now, wherein dendrobium candidum is the superfine product of stem of noble dendrobium class traditional Chinese medicine always, the height approval that the traditional medicine and health care function of current dendrobium candidum has obtained people with like.The stem of noble dendrobium is not only all on the books at tradition classic Chinese medicine books in " the careless warp of legendary god of farming's wood " (25-220 in Christian era), Compendium of Material Medica and " Dictionary of Chinese Pharmacy " (nineteen thirty-five) etc., and be classified as medicinal material it " top grade ", and it is all on the books in all versions of the Pharmacopoeia of the People's Republic of China, there is reinforcing stomach reg fluid, nourishing Yin and clearing heat effect, is applicable to the moon and hinders that body fluid deficiency, dry polydipsia, food few are retched, abnormal heat, the order secretly symptom such as not clear after being ill.The maple bucket be processed into using dendrobium candidum, always as senior guarantor's throat good medicine, enjoys great prestige the ground such as Southeast Asia and China Hong Kong, Macao.
Dendrobium candidum resource wild is at present very rare, and owing to relying on wild resource far can not meet the need of market, therefore artificial planting is the only way solving the market demand.Greatly develop the artificial planting of dendrobium candidum, not only can meet the demand of people to dendrobium candidum, and can play because of minimizing the object protecting wild resource to the dependence of wild resource.Artificial planting dendrobium candidum not only has good market prospects, and also has important meaning to the protection of dendrobium candidum wild resource.
Artificial planting dendrobium candidum, what first will solve is seedling problem.Current artificial planting seedlings of Dendrobium officinale is mainly derived from the test-tube plantlet that Plant Tissue Breeding is produced, and is secondly a small amount of seedling obtained from the plant division of old plant rhizome.Tissue cultures is adopted to carry out Fast-propagation, frequent subculture, easily cause somaclonal variation, thus the original species matter of preservation is lost, even if also there is inter-strain variation in same kind, finally cause the quality of dendrobium candidum kind matter to decline very fast, provenance quality is difficult to ensure, brings very large difficulty to dendrobium candidum quality monitoring.
Develop dendrobium candidum industry, first must reduce planting cost by improving survival rate, improving its seed output and quality simultaneously.Group culturation rapid propagating technology is the main source of current dendrobium candidum artificial planting seedling.This technology utilize gnotobasis produce seedlings of Dendrobium officinale, usually with seed, aseptic seedling seedling stem section, protocorm and artificial seed etc. for explant, by " 1. seed → protocorm → plantlet; 2. seed → protocorm → aseptic seedling stem sections → plantlet; 3. seed → protocorm → callus → clump bud → seedling of taking root; 4. seed → callus → protocorm → plantlet; 5. protocorm → artificial seed → seedling; 6. stem apex → callus → clump bud → seedling of taking root " etc. Regeneration Ways obtain seedling plant.
Adopt seed to be directly seeded into Organic mediacy culture and educate the research of seedling from seed seldom, also successfully do not report more there is no scale breeding technology at present.The object of the invention is to cultivate for dendrobium candidum tissue carry out Fast-propagation, frequent subculture, easily cause somaclonal variation, the original species matter of preservation is lost, the quality of dendrobium candidum kind matter is caused to decline very fast, provenance quality is difficult to ensure, dendrobium candidum quality monitoring difficulty waits and researches and develops large-scale cultivation Seeds of Dendrobium Candidum seedling, change the situation that current dendrobium candidum plantation source of seedling mainly relies on tissue culture propagation test-tube plantlet, the source of seedlings of Dendrobium officinale is enable to return the sexual propagation state in primary border, the original species matter that dendrobium candidum is preserved better is maintained and is continued, improve plantation medicinal material product quality.
Through repeatedly practising, we are by cultivating the capsule of dendrobium candidum elite plant, and the planting seed collecting capsule is sprouted in the organic substrate of routine, and cultivates seedling, supply artificial planting production and application.There is after the Seeds of Dendrobium Candidum seedling direct planting adopting non-Propagations of Teat Tube Seedlings to cultivate the features such as growth is fast, survival rate is high, strong stress resistance, deeply pursue by grower.The large-scale cultivation of Seeds of Dendrobium Candidum seedling; the source of seedlings of Dendrobium officinale is enable to return the sexual propagation state in primary border; the original species matter that dendrobium candidum is preserved better is maintained and is continued; improve plantation medicinal material product quality, promote optimization and the upgrading of agricultural structure further, improve agriculture production benefit.
Summary of the invention
The object of this invention is to provide a kind of breeding method of Seeds of Dendrobium Candidum seedling; the seedling cultivated has the advantages such as growth is fast, survival rate is high, strong stress resistance; achieve the large-scale cultivation of seedling, the original species matter of the dendrobium candidum of better preservation and continuity.
In order to solve the problem, the technical solution adopted in the present invention is:
A method for Seeds of Dendrobium Candidum seedling breeding, comprises the steps:
(1) preparation of seedling matrix material
Select thickness to be suitable for, not have the sawdust of microbial infection, to dry under burning sun or for subsequent use after thermophilic digestion;
(2) cultivation of Seeds of Dendrobium Candidum, collection and storage
Screening kind of a matter resistance, output are high, best in quality, and the dendrobium candidum plant being applicable to local plantation does parent, and carry out people and assist self-pollination to cultivate capsule seed, capsule grew through 4-5 month, present micro-yellow namely can pluck to yellow when it expands position.It is air-dry or dry that the capsule of fresh harvesting is contained in ventilative paper bag, allows Powdered germinating in paper bag, then got up by Powdered seed collection, be stored in the environment of aeration-drying, lucifuge for subsequent use;
(3) laying of seedling bed
In greenhouse or plastic tunnel, arrange culture facility, the sawdust matrix that thickness is suitable is laid on seedling bed equably, irrigates matrix, and carries out appropriate smooth suppression, make stromal thickness at 3-8cm with clean well water or mountain spring water;
(4) sow
Use clean air, water, sawdust, peat or earth harmless material medium by pulverous Seeds of Dendrobium Candidum sowing of evenland to seedling medium surface;
(5) cultivation of seedling from seed
Regulable control is at natural lighting 1000-5000lx, temperature 25-30 DEG C, germination and emergence under the control environment of relative moisture 65-80%; When seedling grows 2 leaves, seedling is moved on to good permeability in time, surface is equipped with the thick thickness of 1-2cm and is suitable on the container for plant growth of sawdust, by spacing in the rows 3-5cm, the specification field planting of line-spacing 6-8cm, intensive care, the plantlet that seedling grows up to height more than 5cm survives the rear liquid organic fertilizer spraying 0.05-0.1% for every 7-10 days, until can carry out transplanting plantation.
Seedling matrix material described in step (1) refers to that thickness is suitable for, does not have the sawdust of microbial infection, dries or for subsequent use after thermophilic digestion under burning sun.Seeds of Dendrobium Candidum germination process, seedling development process all may experience a very long time, Seeds of Dendrobium Candidum does not have endosperm, in Powdered, require high to sprouting and growing environment, must just be conducive to germination and growth in the matrix of aseptic, few bacterium and become seedling, in the matrix environment existed there being bacterium colony, be difficult to protocorm and the seedling plant of finding sprouting.
Cultivation capsule seed described in step (2) refers to that screening kind of matter resistance, output are high, best in quality, the dendrobium candidum plant being applicable to local plantation does parent, carry out people and assist autophilous capsule, capsule grew through 4-5 month, presents micro-yellow to yellow side can pluck when it expands position.It is consistent that this step defines isozygotying of cultivated seedling from seed germplasm origin, and seed physiology maturity is high, and germinating capacity is strong.
Seeds of Dendrobium Candidum described in step (2) collects and stores that to refer to that the fresh capsule plucked need be contained in ventilative paper bag air-dry or dry, allow Powdered germinating in paper bag, then Powdered seed collection is got up, be stored in the environment of aeration-drying, lucifuge for subsequent use.This step defines cultivated seed and meet seed collection condition of storage in collection storage process, ensures that seed is in high germination power state.
The laying of the seedling bed described in step (3) refers to that culture facility must be located in greenhouse or plastic tunnel, the matrix be layered on seedling bed refers to the sawdust that thickness is suitable, sawdust matrix carries out appropriate smooth suppression after irrigating with clean well water or mountain spring water, and stromal thickness is at 3-8cm.This step ensure that Seeds of Dendrobium Candidum is sprouted, matrix environment required for seedling development, suitable illumination, moisture and nutrition continued growth can be obtained after seed germination and develop into seedling, avoid seed to enter in too loose or concavo-convex matrix losing germination and growth opportunity, then losing germinating capacity.
Seeds of Dendrobium Candidum described in step (4) needs to be seeded into seedling medium surface equably.This step ensure that Seeds of Dendrobium Candidum is sprouted, matrix environment required for seedling development, suitable illumination, moisture and nutrition continued growth can be obtained after seed germination and develop into seedling, avoid seed too crowded, be difficult to after sprouting obtain enough nutrition and stop further growth growing.
Control environment described in step (5) refers to 1000-5000lx natural lighting, temperature 25-30 DEG C, relative moisture 65-80%.This step ensure that Seeds of Dendrobium Candidum is sprouted required environmental condition and can obtain suitable illumination, temperature, moisture and nutrition etc. after sprouting, and continued growth develops into seedling, improves seed sprouting planting percent.
Field planting specification described in step (5) refers to by spacing in the rows 3-5cm, and the specification of line-spacing 6-8cm carries out field planting.Dendrobium candidum seedling has clustering growth, in time seedling is pressed certain specification transplant planting, is convenient to strengthen the intensive care to seedling, is bred as strong sprout.Avoid the surging competitive influence of seedling, increase former seedling bed Seed germination and emergence rate.
Liquid organic fertilizer described in step (5), its preparation method is: peanut press pulp 10kg, water 100kg, EM bacterium original bacteria liquid 5-10kg mixing after in closed container abundant fermentation maturity, do not have the liquid of residue to use after getting filtration.
The invention has the beneficial effects as follows:
(1) by cultivating the capsule of dendrobium candidum elite plant, the planting seed collecting capsule is sprouted in the organic substrate of routine, cultivate seedling, have employed seed to be directly seeded into organic substrate and to cultivate the tradition of seedling and advanced method, and compare with plantlet in vitro, the growth showed after seedling direct planting is fast, survival rate is high, the advantage of strong stress resistance is extremely obvious;
(2) achieve the large-scale cultivation of Seeds of Dendrobium Candidum seedling, the source of seedling can return the sexual propagation state in primary border, and the original species matter that dendrobium candidum is preserved better is maintained and continues, and improves plantation medicinal material product quality;
(3), when the seedling that this method is cultivated is planted, stable high yield, product quality is excellent, can realize lasting for years and gather.
Embodiment
Be described in further details the present invention below by embodiment, these embodiments are only used for the present invention is described, do not limit the scope of the invention.
embodiment 1
A method for Seeds of Dendrobium Candidum seedling breeding, comprises the steps:
(1) preparation of seedling matrix material
Select thickness to be suitable for, not have the sawdust of microbial infection, to dry under burning sun or for subsequent use after thermophilic digestion;
(2) cultivation of Seeds of Dendrobium Candidum, collection and storage
Screening kind of matter resistance, output are high, best in quality, the dendrobium candidum plant being applicable to local plantation does parent, carrying out people assists self-pollination to cultivate capsule seed, and capsule grew through 4 months, presents micro-yellow and namely can pluck capsule when it expands position to yellow.It is air-dry or dry that capsule is contained in ventilative paper bag, allows Powdered germinating in paper bag, then got up by Powdered seed collection, be stored in the environment of aeration-drying, lucifuge for subsequent use.
(3) laying of seedling bed
In greenhouse or plastic tunnel, arrange culture facility, the sawdust matrix that thickness is suitable is laid on seedling bed equably, and irrigate matrix with clean well water or mountain spring water, and carry out appropriate smooth suppression, stromal thickness is at 3cm;
(4) sow
Use clean air, water, sawdust, peat or earth harmless material medium by pulverous Seeds of Dendrobium Candidum sowing of evenland to seedling medium surface;
(5) cultivate
Regulable control germination and emergence under the control environment of 1000-5000lx natural lighting, temperature 25-30 DEG C, relative moisture 65-80%; When seedling grows 2 leaves, seedling is moved on to good permeability in time, surface is equipped with the thick thickness of 1cm and is suitable on the container for plant growth of sawdust, by spacing in the rows 3cm, the specification field planting of line-spacing 8cm, intensive care, the plantlet that seedling grows up to height more than 5cm survives the liquid organic fertilizer that latter every 7 days spray 0.05%, until can carry out transplanting plantation.
embodiment 2
A method for Seeds of Dendrobium Candidum seedling breeding, comprises the steps:
(1) preparation of seedling matrix material
Select thickness to be suitable for, not have the sawdust of microbial infection, to dry under burning sun or for subsequent use after thermophilic digestion;
(2) cultivation of Seeds of Dendrobium Candidum, collection and storage
Screening kind of matter resistance, output are high, best in quality, the dendrobium candidum plant being applicable to local plantation does parent, carrying out people assists self-pollination to cultivate capsule seed, and capsule grew through 5 months, presents micro-yellow and namely can pluck capsule when it expands position to yellow.It is air-dry or dry that capsule is contained in ventilative paper bag, allows Powdered germinating in paper bag, then got up by Powdered seed collection, be stored in the environment of aeration-drying, lucifuge for subsequent use.
(3) laying of seedling bed
In greenhouse or plastic tunnel, arrange culture facility, the matrix such as the sawdust suitable by thickness are laid on seedling bed equably, and irrigate matrix with clean well water or mountain spring water, and carry out appropriate smooth suppression, stromal thickness is at 8cm;
(4) sow
Use clean air, water, sawdust, peat or earth harmless material medium by pulverous Seeds of Dendrobium Candidum sowing of evenland to seedling medium surface;
(5) cultivate
Regulable control germination and emergence under the control environment of 1000-5000lx natural lighting, temperature 25-30 DEG C, relative moisture 65-80%; When seedling grows 2 leaves, seedling is moved on to good permeability in time, surface is equipped with the thick thickness of 2cm and is suitable on the container for plant growth of sawdust, by spacing in the rows 3cm, the specification field planting of line-spacing 6cm, intensive care, the plantlet that seedling grows up to height more than 5cm survives the liquid organic fertilizer that latter every 10 days spray 0.1%, until can carry out transplanting plantation.
embodiment 3
A method for Seeds of Dendrobium Candidum seedling breeding, comprises the steps:
(1) preparation of seedling matrix material
Select thickness to be suitable for, not have the sawdust of microbial infection, to dry under burning sun or for subsequent use after thermophilic digestion;
(2) cultivation of Seeds of Dendrobium Candidum, collection and storage
Screening kind of matter resistance, output are high, best in quality, the dendrobium candidum plant being applicable to local plantation does parent, carrying out people assists self-pollination to cultivate capsule seed, and capsule grew through 4 months, presents micro-yellow and namely can pluck capsule when it expands position to yellow.It is air-dry or dry that capsule is contained in ventilative paper bag, allows Powdered germinating in paper bag, then got up by Powdered seed collection, be stored in the environment of aeration-drying, lucifuge for subsequent use.
(3) laying of seedling bed
In greenhouse or plastic tunnel, arrange culture facility, the matrix such as the sawdust suitable by thickness are laid on seedling bed equably, and irrigate matrix with clean well water or mountain spring water, and carry out appropriate smooth suppression, stromal thickness is at 5cm;
(4) sow
Use clean air, water, sawdust, peat or earth harmless material medium by pulverous Seeds of Dendrobium Candidum sowing of evenland to seedling medium surface;
(5) cultivate
Regulable control germination and emergence under the control environment of 1000lx-5000lx natural lighting, temperature 25 DEG C-30 DEG C, relative moisture 65%-80%; When seedling grows 2 leaves, seedling is moved on to good permeability in time, surface is equipped with the thick thickness of 1.5cm and is suitable on the container for plant growth of sawdust, by spacing in the rows 4cm, the specification field planting of line-spacing 7cm, intensive care, the plantlet that seedling grows up to height more than 5cm survives the liquid organic fertilizer that latter every 8 days spray 0.07%, until can carry out transplanting plantation.
embodiment 4
. a kind of method of Seeds of Dendrobium Candidum seedling breeding, comprises the steps:
(1) preparation of seedling matrix material
Select thickness to be suitable for, not have the sawdust of microbial infection, to dry under burning sun or for subsequent use after thermophilic digestion;
(2) cultivation of Seeds of Dendrobium Candidum, collection and storage
Screening kind of matter resistance, output are high, best in quality, the dendrobium candidum plant being applicable to local plantation does parent, carrying out people assists self-pollination to cultivate capsule seed, and capsule grew through 4 months, presents micro-yellow and namely can pluck capsule when it expands position to yellow.It is air-dry or dry that capsule is contained in ventilative paper bag, allows Powdered germinating in paper bag, then got up by Powdered seed collection, be stored in the environment of aeration-drying, lucifuge for subsequent use.
(3) laying of seedling bed
In greenhouse or plastic tunnel, arrange culture facility, the matrix such as the sawdust suitable by thickness are laid on seedling bed equably, and irrigate matrix with clean well water or mountain spring water, and carry out appropriate smooth suppression, stromal thickness is at 6cm;
(4) sow
Use clean air, water, sawdust, peat or earth harmless material medium by pulverous Seeds of Dendrobium Candidum sowing of evenland to seedling medium surface;
(5) cultivate
Regulable control germination and emergence under the control environment of 1000-5000lx natural lighting, temperature 25-30 DEG C, relative moisture 65-80%; When seedling grows 2 leaves, seedling is moved on to good permeability in time, surface is equipped with the thick thickness of 2cm and is suitable on the container for plant growth of sawdust, by spacing in the rows 4cm, the field planting of line-spacing 6cm specification, intensive care, the plantlet that seedling grows up to height more than 5cm survives the liquid organic fertilizer that latter every 9 days spray 0.05%, until can carry out transplanting plantation.
embodiment 5
A method for Seeds of Dendrobium Candidum seedling breeding, comprises the steps:
(1) preparation of seedling matrix material
Select thickness to be suitable for, not have the sawdust of microbial infection, to dry under burning sun or for subsequent use after thermophilic digestion;
(2) cultivation of Seeds of Dendrobium Candidum, collection and storage
Screening kind of matter resistance, output are high, best in quality, the dendrobium candidum plant being applicable to local plantation does parent, carrying out people assists self-pollination to cultivate capsule seed, and capsule grew through 4 months, presents micro-yellow and namely can pluck capsule when it expands position to yellow.It is air-dry or dry that capsule is contained in ventilative paper bag, allows Powdered germinating in paper bag, then got up by Powdered seed collection, be stored in the environment of aeration-drying, lucifuge for subsequent use.
(3) laying of seedling bed
In greenhouse or plastic tunnel, arrange culture facility, the matrix such as the sawdust suitable by thickness are laid on seedling bed equably, and irrigate matrix with clean well water or mountain spring water, and carry out appropriate smooth suppression, stromal thickness is at 7cm;
(4) sow
Use clean air, water, sawdust, peat or earth harmless material medium by pulverous Seeds of Dendrobium Candidum sowing of evenland to seedling medium surface;
(5) cultivate
Regulable control germination and emergence under the control environment of 1000-5000lx natural lighting, temperature 25-30 DEG C, relative moisture 65-80%; When seedling grows 2 leaves, seedling is moved on to good permeability in time, surface is equipped with the thick thickness of 1cm and is suitable on the container for plant growth of sawdust, by spacing in the rows 5cm, the specification field planting of line-spacing 6cm, intensive care, the plantlet that seedling grows up to height more than 5cm survives the liquid organic fertilizer that latter every 10 days spray 0.08%, until can carry out transplanting plantation.
Claims (1)
1. a breeding method for Seeds of Dendrobium Candidum seedling, is characterized in that: comprise the steps:
(1) preparation of seedling matrix material
Select thickness to be suitable for, not have the sawdust of microbial infection, to dry under burning sun or for subsequent use after thermophilic digestion;
(2) cultivation of Seeds of Dendrobium Candidum, collection and storage
Screening kind of a matter resistance, output are high, best in quality, and the dendrobium candidum plant being applicable to local plantation does parent, and carry out people and assist self-pollination to cultivate capsule seed, capsule grew through 4-5 month, present micro-yellow namely pluck to yellow when it expands position; It is air-dry or dry that capsule is contained in ventilative paper bag, allows Powdered germinating in paper bag, then got up by Powdered seed collection, be stored in the environment of aeration-drying, lucifuge for subsequent use;
(3) laying of seedling bed
In greenhouse or plastic tunnel, arrange culture facility, the sawdust matrix that thickness is suitable is laid on seedling bed equably, irrigates matrix, and carries out appropriate smooth suppression, make stromal thickness at 3-8cm with clean well water or mountain spring water;
(4) sow
Use clean air, water, sawdust, peat or earth harmless material medium that pulverous Seeds of Dendrobium Candidum is seeded into seedling medium surface equably;
(5) cultivation of seedling from seed
Regulable control is at natural lighting 1000-5000lx, temperature 25-30 DEG C, germination and emergence under the control environment of relative moisture 65-80%; When seedling grows 2 leaves, seedling is moved on to good permeability in time, surface is equipped with the thick thickness of 1-2cm and is suitable on the container for plant growth of sawdust, by spacing in the rows 3-5cm, the specification field planting of line-spacing 6-8cm, intensive care, survive the rear liquid organic fertilizer spraying 0.05-0.1% for every 7-10 days, its preparation method is: peanut press pulp 10kg, water 100kg, EM bacterium original bacteria liquid 5-10kg mixing after in closed container abundant fermentation maturity, do not have the liquid of residue to use after getting filtration.
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| CN103999672B (en) * | 2014-05-22 | 2016-03-23 | 普洱市民族传统医药研究所 | A kind of method of non-polluted planting dendrobium candidum |
| CN105875138A (en) * | 2015-04-29 | 2016-08-24 | 广西桂平市仙宝园铁皮石斛有限责任公司 | Method for breeding direct seeding seedlings through natural seeding of dendrobe seeds |
| CN105359983A (en) * | 2015-12-24 | 2016-03-02 | 陕西师范大学 | Dendrobium officinale Kimura et Migo protocorm direct-seeding method |
| CN106258956B (en) * | 2016-08-08 | 2018-06-15 | 四川千草生物技术股份有限公司 | A kind of dendrobium candidum pedigree breeding and field transplantation method |
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| CN101695272B (en) * | 2009-10-26 | 2011-03-30 | 重庆市中药研究院 | Natural reproduction method for dendrobium |
| CN102440130A (en) * | 2011-09-22 | 2012-05-09 | 澄思源生物科技（上海）有限公司 | Method for natural propagation of dendrobium officinale seeds |
| CN103004409A (en) * | 2012-12-07 | 2013-04-03 | 安徽新津铁皮石斛开发有限公司 | Method for natural wild dendrobium officinale Kimura et Migo cultivation management |
| CN103004421B (en) * | 2012-12-14 | 2014-04-16 | 茂名市惠生源生物科技有限公司 | Planting method of famous and precious Chinese herb medicine dendrobium officinale |
| CN103004445B (en) * | 2013-01-06 | 2014-07-23 | 戴亚峰 | Method for directly seedling dendrobe seedlings by using plastic greenhouse |
| CN103283433B (en) * | 2013-03-27 | 2015-04-15 | 桂平市荣成石斛种植有限公司 | Propagation method for dendrobium candidum seeds |
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