CN103004445B - Method for directly seedling dendrobe seedlings by using plastic greenhouse - Google Patents
Method for directly seedling dendrobe seedlings by using plastic greenhouse Download PDFInfo
- Publication number
- CN103004445B CN103004445B CN201310004397.7A CN201310004397A CN103004445B CN 103004445 B CN103004445 B CN 103004445B CN 201310004397 A CN201310004397 A CN 201310004397A CN 103004445 B CN103004445 B CN 103004445B
- Authority
- CN
- China
- Prior art keywords
- seedbed
- stem
- matrix
- noble dendrobium
- seedling
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/25—Greenhouse technology, e.g. cooling systems therefor
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
- Cultivation Of Plants (AREA)
Abstract
A method for directly seedling dendrobe seedlings by using a plastic greenhouse comprises the following steps: constructing a greenhouse; making a seedling bed; preparing a base material and performing sterilization, which can be realized by mixing pine barks and sheep manure, filtering the pine barks and the sheep manure through a screen after completely fermenting the pine barks and the sheep manure, soaking the pine barks and the sheep manure by using carbendazim, spreading the pine barks and the sheep manure on the seedling bed, placing the pine barks and the sheep manure on the seedling bed for 5-10 days, and controlling the water content; inoculating the symbiotic bacteria, which can be realized by taking a two years old base material of the dendrobe and uniformly scattering the two years old substrate on the seedling bed on which a new substrate is laid; pre-treating seeds, which can be realized by completely moisturizing the seeds by using sterile water containing naphthyl acetic acid; sterilizing the seeds in the greenhouse, which can be realized by fumigating every cubic meter of seeds by using 4 g mushroom protector, fumigating the seeds for one time before sowing, and fumigating the seeds every half a month in the future; scattering, which can be realized by after the seeds are scattered and before the seedlings are transplanted, controlling temperature in the greenhouse, water content of the seedling bed and air humidity, treating the seeds by weak lights within the first month of the scattering, and gradually enhancing illumination after budding; managing water, fertilizers and diseases of the seedlings; and transplanting the seedlings. The method for directly seedling the dendrobe seedlings by using the plastic greenhouse, provided by the invention has the advantages that as the dendrobe are transplanted after directly becoming to the seedlings, the seedling breeding cost is effectively reduced, the seedling rate of the seeds is more than 90 percent, and the transplanting survival rate of the seedlings is 100 percent.
Description
Technical field
The present invention relates to the growing and cultivation field of the stem of noble dendrobium, be specifically related to the method for the live stem of noble dendrobium seedling of a kind of plastic tunnel.
Background technology
Dendrobium the orchid family herbaceos perennial, the common kind of the stem of noble dendrobium has: HERBA DENDROBII, dendrobium candidum, purple dendrobium, Dendrobidium huoshanness etc., the curative effect such as the part stem of noble dendrobium can be used as medicine, and human body is driven to solution abnormal heat, beneficial smart reinforcing yin essence.
Dendrobidium huoshanness is commonly called as " rice dry measure used in former times ", is the superfine product in the stem of noble dendrobium, is mainly distributed in the Huoshan in the west and south, Anhui.Because of the compositions such as its contained alkaloid, thick polysaccharide, amino acid, trace element have strong kidney benefit essence, thick stomach, inhibition tumor cell, hypoglycemic, strengthen the effects such as immunity, alleviating physical fatigue, be regarded as first of China nine large " celestial careless " always.For a long time, because the growth conditions of Dendrobidium huoshanness and harshness thereof and people excessively pluck it, cause these species endangered.
Numerous companies and scientific research institutions, by modern biotechnology, have realized conservation and rational exploitation and utilization to the stem of noble dendrobium.Its general process is: tissue culture, and hardening, domestication, transplants, gathers, deep processing.
In this process of tissue culture, relating to medium makes, aseptic access explant, protocorm is aseptic to be turned point, and Bud Differentiation is aseptic to be turned point, seedling is aseptic to be turned point, middle seedling is aseptic to be turned point, and seedlings are aseptic to be turned point, the steps such as culturing room's management, relate to thus point the Miaos person's recruitment and management, the construction of medium atelier, the construction in sterile working workshop, the construction of culturing room; And in hardening, domestication process, relate to related personnel's recruitment and management, the construction of intelligent greenhouse and domestication booth; The survival rate of simultaneously transplanting is low, is about 70%-75% even lower.In sum, prior art aspect requires enterprise in human and material resources, financial resources, all will have sizable input, causes thus that entreprise cost is large, burden is heavy, one of transplanting survival rate primary factor that has become the development of restriction stem of noble dendrobium industrial scale on the low side simultaneously.
Summary of the invention
Technical problem to be solved by this invention be to provide a kind of effectively reduce entreprise cost, seed planting percent up to more than 90% and transplanting survival rate up to the method for 100% the live stem of noble dendrobium seedling of plastic tunnel.
The present invention solves the problems of the technologies described above by the following technical solutions: the method for the live stem of noble dendrobium seedling of a kind of plastic tunnel, comprises the following steps:
1, build booth: the specification of booth does not have special requirement, people can enter to operate can.Plastic covering film and sunshade net above booth, for rainwater-proof and stop sunray, make the highest 20000 luxs that are no more than of illumination.Optimize, for easy to operate, described greenhouse is high 2.5～4 meters, wide 5～7 meters.Also be as the scheme of optimizing, for rain proofness and stop that sunshine effect is better, above booth, cover the sunshade net of 6～9 thick plastic foils and sunshade rate 70 ﹪～90%.
2, make seedbed: seedbed is the built on stilts plastic deeping in bottom, be beneficial to bottom and ventilate, the specification in seedbed does not have special requirement, can put booth and handled easily into.Optimize, plastic deeping rides on the fixed mount of angle steel welding, and built on stilts with hollow concrete block under angle steel.Optimize, for easy to operate, seedbed is wide 1～2 meter, and length is as the criterion with canopy length.
3, matrix and sterilization: pine bark and sheep excrement are pressed to (3～5): 1 mixes, rotten rear 3～5 object screen clothes of crossing thoroughly that ferment, with the carbendazim immersions of 600 times 3～5 minutes, then pave on seedbed, 8～12 centimetres of thickness, place 5～10 days, control seedbed moisture content at 60 ﹪～65 ﹪.
4, fungal component inoculation: get 2 years above old matrix of the cultivation stem of noble dendrobium, cross 3～5 object screen clothes, spread the consumption of 1～1.5 kilogram of old matrix by every square metre, old matrix is sprinkling upon on the seedbed of completing new matrix uniformly.The stem of noble dendrobium is in outdoor growth all with symbiosis fungi, and stem of noble dendrobium seed is not because there is no endosperm, namely do not supply the nutriment of its growth, and the root symbiosis mycosymbiosis must grow with the stem of noble dendrobium time, relies on it that nutrient is provided, and could sprout.
5, seed pretreatment: by the alcohol-pickled sterilization of 75 ﹪ 4～6 minutes for ripe stem of noble dendrobium capsule, then cut open, seed in fruit is poured on and contains 2 Hao Ke ∕ and rise in the sterile water that methyl α-naphthyl acetate (NAA) and 0.5 Hao Ke ∕ rise 6-benzyl aminopurine (6-BA) and infiltrate 4～6 hours, allow seed coat be filled containing hormone sterile water.
6, sterilization in canopy: the guarantor mushroom king that every cubic metre of use is 4 grams is stifling, before sowing stifling once, later stifling once every two weeks;
7, broadcast sowing: start to sow the spring turning after warming up at outside air temperature, also be outdoor temperature 17～30 degree, first on seedbed, get 0.8～1.2 kilogram of matrix, mix 8～12 seeds that soaked hormone, then after mixing thoroughly, be sprinkling upon on seedbed, area is 8～12 square metres.
8, temperature, light, humidity control: after broadcasting sowing,, to transplanting, temperature of shed is controlled between 18 DEG C～30 DEG C, the most suitable with 28 DEG C; Seedbed moisture control is at 60%～65 ﹪, and air humidity maintains 60 ﹪～80 ﹪; The first month of broadcasting sowing, is used low light level processing, and maximum intensity is no more than 2000 luxs, strengthens gradually illumination after sprouting, but the highest 20000 luxs that are no more than.
9, water, fertilizer, plant disease management: maintain seedbed moisture content 60%～65 ﹪ with sprinkling equipment, air humidity 60 ﹪～80 ﹪, wait the stem of noble dendrobium to extract out after two leaves, spray weekly a flower foliage fertilizer of 1 to 2 time 1 ‰; Daily disease by within every ten days, spray one time 600 times carbendazim or the thiophanate methyl of 750 times.
10, transplant: the spring of Second Year, the stem of noble dendrobium seedling of complete root, stem, leaf will be possessed, rise together with matrix one digging, optimize, transplant by the seeding row spacing of 15 centimetres of 10 cm x, can certainly transplant according to other suitable seeding row spacings, this is all prior art, and follow-up management is by existing planting type.
The invention has the advantages that: directly stem of noble dendrobium high quality seed is broadcast on treated good seedbed, by the cultivation management of science, directly after seedling, transplanted again.Can effectively reduce entreprise cost, and seed planting percent is up to more than 90%, and transplanting survival rate is up to 100%.Can live Dendrobidium huoshanness, the various stem of noble dendrobium kind seedlings, particularly Dendrobidium huoshanness such as HERBA DENDROBII, dendrobium candidum, purple dendrobium, there is huge commercial promise.
Embodiment
Embodiment mono-
A method for the live Dendrobidium huoshanness seedling of plastic tunnel, comprises the following steps:
1, build booth: greenhouse is high 3 meters, wide 6 meters, above booth, cover the sunshade net of 8 thick plastic foils and sunshade rate 90 ﹪.
2, make seedbed: seedbed is the built on stilts plastic deeping in bottom, optimization, plastic deeping rides on the fixed mount of angle steel welding, and built on stilts with hollow concrete block under angle steel, and seedbed is wide 1.5 meters, and length is as the criterion with canopy length.
3, matrix and sterilization: pine bark and sheep excrement are pressed to 4:1 and mix, fermentation is rotten crosses 4 object screen clothes after thoroughly, soaks 4 minutes with the carbendazim of 600 times, then paves on seedbed, and 10 centimetres of thickness, place 7 days, and control seedbed moisture content is at 60 ﹪.
4, fungal component inoculation: get 2 years above old matrix of the cultivation stem of noble dendrobium, cross 4 object screen clothes, spread the consumption of 1 kilogram of old matrix by every square metre, old matrix is sprinkling upon on the seedbed of completing new matrix uniformly.
5, seed pretreatment: by the alcohol-pickled sterilization of 75 ﹪ 5 minutes for ripe stem of noble dendrobium capsule, then cut open, seed in fruit is poured on and contains 2 Hao Ke ∕ and rise in the sterile water that methyl α-naphthyl acetate (NAA) and 0.5 Hao Ke ∕ rise 6-benzyl aminopurine (6-BA) and infiltrate 5 hours, allow seed coat be filled containing hormone sterile water.
6, sterilization in canopy: the guarantor mushroom king that every cubic metre of use is 4 grams is stifling, before sowing stifling once, later stifling once every two weeks;
7, broadcasting sowing: start sowing the spring turning after warming up at outside air temperature, is also outdoor temperature 17～25 degree, first on seedbed, gets 1 kilogram of matrix, mixes 10 seeds that soaked hormone, after then mixing thoroughly, is sprinkling upon on seedbed, and area is 10 square metres.
8, temperature, light, humidity control: after broadcasting sowing,, to transplanting, temperature of shed is controlled at 28 DEG C, and seedbed moisture control is at 60 ﹪, and air humidity maintains 70 ﹪; The first month of broadcasting sowing, is used low light level processing, and maximum intensity is no more than 2000 luxs, strengthens gradually illumination after sprouting, but the highest 20000 luxs that are no more than.
9, water, fertilizer, plant disease management: maintain seedbed moisture content 60 ﹪ with sprinkling equipment, air humidity 70 ﹪, wait the stem of noble dendrobium to extract out after two leaves, spray weekly a flower foliage fertilizer of 1 time 1 ‰; Daily disease is by within every ten days, spraying the carbendazim of 600 times one time.
10, transplant: in the spring of Second Year, by possessing the stem of noble dendrobium seedling of complete root, stem, leaf, rise together with matrix one digging, transplant by the seeding row spacing of 15 centimetres of 10 cm x.Follow-up management is by existing planting type.
Embodiment bis-
A method for the live HERBA DENDROBII seedling of plastic tunnel, comprises the following steps:
1, build booth: described greenhouse is high 2.5 meters, wide 5 meters, above booth, cover the sunshade net of 6 thick plastic foils and sunshade rate 70 ﹪.
2, make seedbed: seedbed is the built on stilts plastic deeping in bottom, and plastic deeping rides on the fixed mount of angle steel welding, and built on stilts with hollow concrete block under angle steel, seedbed is wide 1 meter, and length is as the criterion with canopy length.
3, matrix and sterilization: pine bark and sheep excrement are pressed to 3:1 and mix, fermentation is rotten crosses 3 object screen clothes after thoroughly, soaks 5 minutes with the carbendazim of 600 times, then paves on seedbed, and 8 centimetres of thickness, place 5 days, and control seedbed moisture content is at 65 ﹪.
4, fungal component inoculation: get 2 years above old matrix of the cultivation stem of noble dendrobium, cross 3 object screen clothes, spread the consumption of 1.2 kilograms of old matrix by every square metre, old matrix is sprinkling upon on the seedbed of completing new matrix uniformly.The stem of noble dendrobium is in outdoor growth all with symbiosis fungi, and stem of noble dendrobium seed is not because there is no endosperm, namely do not supply the nutriment of its growth, and the root symbiosis mycosymbiosis must grow with the stem of noble dendrobium time, relies on it that nutrient is provided, and could sprout.
5, seed pretreatment: by the alcohol-pickled sterilization of 75 ﹪ 4 minutes for ripe stem of noble dendrobium capsule, then cut open, seed in fruit is poured on and contains 2 Hao Ke ∕ and rise in the sterile water that methyl α-naphthyl acetate (NAA) and 0.5 Hao Ke ∕ rise 6-benzyl aminopurine (6-BA) and infiltrate 4 hours, allow seed coat be filled containing hormone sterile water.
6, sterilization in canopy: the guarantor mushroom king that every cubic metre of use is 4 grams is stifling, before sowing stifling once, later stifling once every two weeks;
7, broadcasting sowing: start sowing the spring turning after warming up at outside air temperature, is also outdoor temperature 17～25 degree, first on seedbed, gets 0.8 kilogram of matrix, mixes 8 seeds that soaked hormone, after then mixing thoroughly, is sprinkling upon on seedbed, and area is 8 square metres.
8, temperature, light, humidity control: after broadcasting sowing,, to transplanting, temperature of shed is controlled at 18 DEG C, and seedbed moisture control is 65%, and air humidity maintains 70 ﹪; The first month of broadcasting sowing, is used low light level processing, and maximum intensity is no more than 2000 luxs, strengthens gradually illumination after sprouting, but the highest 20000 luxs that are no more than.
9, water, fertilizer, plant disease management: maintain seedbed moisture content 65% with sprinkling equipment, air humidity 70 ﹪, wait the stem of noble dendrobium to extract out after two leaves, spray weekly a flower foliage fertilizer of 1 time 1 ‰; Daily disease is by within every ten days, spraying the thiophanate methyl of 750 times one time.
10, transplant: in the spring of Second Year, by possessing the stem of noble dendrobium seedling of complete root, stem, leaf, rise together with matrix one digging, transplant by the seeding row spacing of 12 centimetres of 8 cm x.Follow-up management is by existing planting type.
Embodiment tri-
A method for the live dendrobium candidum seedling of plastic tunnel, comprises the following steps:
1, build booth: described greenhouse is high 4 meters, wide 7 meters, above booth, cover the sunshade net of 9 thick plastic foils and sunshade rate 90 ﹪.
2, make seedbed: seedbed is the built on stilts plastic deeping in bottom, and plastic deeping rides on the fixed mount of angle steel welding, and built on stilts with hollow concrete block under angle steel, seedbed is wide 2 meters, and length is as the criterion with canopy length.
3, matrix and sterilization: pine bark and sheep excrement are pressed to 5:1 and mix, fermentation is rotten crosses 5 object screen clothes after thoroughly, soaks 3 minutes with the carbendazim of 550 times, then paves on seedbed, and 12 centimetres of thickness, place 10 days, and control seedbed moisture content is at 62 ﹪.
4, fungal component inoculation: get 2 years above old matrix of the cultivation stem of noble dendrobium, cross 5 object screen clothes, spread the consumption of 1.5 kilograms of old matrix by every square metre, old matrix is sprinkling upon on the seedbed of completing new matrix uniformly.The stem of noble dendrobium is in outdoor growth all with symbiosis fungi, and stem of noble dendrobium seed is not because there is no endosperm, namely do not supply the nutriment of its growth, and the root symbiosis mycosymbiosis must grow with the stem of noble dendrobium time, relies on it that nutrient is provided, and could sprout.
5, seed pretreatment: by the alcohol-pickled sterilization of 75 ﹪ 6 minutes for ripe stem of noble dendrobium capsule, then cut open, seed in fruit is poured on and contains 2 Hao Ke ∕ and rise in the sterile water that methyl α-naphthyl acetate (NAA) and 0.5 Hao Ke ∕ rise 6-benzyl aminopurine (6-BA) and infiltrate 6 hours, allow seed coat be filled containing hormone sterile water.
6, sterilization in canopy: the guarantor mushroom king that every cubic metre of use is 4 grams is stifling, before sowing stifling once, later stifling once every two weeks;
7, broadcasting sowing: start sowing the spring turning after warming up at outside air temperature, is also outdoor temperature 17～25 degree, first on seedbed, gets 1.2 kilograms of matrix, mixes 12 seeds that soaked hormone, after then mixing thoroughly, is sprinkling upon on seedbed, and area is 12 square metres.
8, temperature, light, humidity control: after broadcasting sowing,, to transplanting, temperature of shed is controlled at 25 DEG C, and seedbed moisture control is 62%, and air humidity maintains 80 ﹪; The first month of broadcasting sowing, is used low light level processing, and maximum intensity is no more than 2000 luxs, strengthens gradually illumination after sprouting, but the highest 20000 luxs that are no more than.
9, water, fertilizer, plant disease management: maintain seedbed moisture content 62% with sprinkling equipment, air humidity 80 ﹪, wait the stem of noble dendrobium to extract out after two leaves, spray weekly a flower foliage fertilizer of 2 times 1 ‰; Daily disease is by within every ten days, spraying the thiophanate methyl of 750 times one time.
10, transplant: in the spring of Second Year, by possessing the stem of noble dendrobium seedling of complete root, stem, leaf, rise together with matrix one digging, transplant by the seeding row spacing of 17 centimetres of 12 cm x.Follow-up management is by existing planting type.
The foregoing is only the preferred embodiment of the invention; not in order to limit the invention; any amendment of doing within all spirit in the invention and principle, be equal to and replace and improvement etc., within all should being included in the protection domain of the invention.
Claims (7)
1. a method for the live stem of noble dendrobium seedling of plastic tunnel, is characterized in that: comprise the following steps:
Step 1, build booth: the highest 20000 luxs that are no more than of illumination in booth;
Step 2, making seedbed: seedbed is the built on stilts plastic deeping in bottom;
Step 3, matrix and sterilization: pine bark and sheep excrement are pressed to (3～5): 1 mixes, rotten rear 3～5 object screen clothes of crossing thoroughly that ferment, with the carbendazim immersions of 600 times 3～5 minutes, then pave on seedbed, 8～12 centimetres of thickness, place 5～10 days, and moisture control is at 60 ﹪～65 ﹪;
Step 4, fungal component inoculation: get 2 years above old matrix of the cultivation stem of noble dendrobium, cross 3～5 object screen clothes, spread the consumption of 1～1.5 kilogram of old matrix by every square metre, old matrix is sprinkling upon uniformly on the seedbed of completing new matrix;
Step 5, seed pretreatment: by the alcohol-pickled sterilization of 75 ﹪ 4～6 minutes for ripe stem of noble dendrobium capsule, then cut open, the seed in fruit is poured in the sterile water that contains 2 mg/litre methyl α-naphthyl acetates and 0.5 mg/litre 6-benzyl aminopurine and infiltrates 4～6 hours;
Sterilization in step 6, canopy: the guarantor mushroom king that every cubic metre of use is 4 grams is stifling, before sowing stifling once, later stifling once every two weeks;
Step 7, broadcast sowing: turn and start sowing in spring after warm at outside air temperature, also be 17～30 DEG C of outdoor temperatures, first on seedbed, get 0.8～1.2 kilogram of matrix, mix 8～12 seeds that soaked hormone, then after mixing thoroughly, be sprinkling upon on seedbed, area is 8～12 square metres;
Step 8, temperature, light, humidity control: before extremely transplanting after broadcasting sowing, temperature of shed is controlled between 18 DEG C～30 DEG C, seedbed moisture control is at 60%～65 ﹪, air humidity maintains 80 ﹪～90 ﹪, the first month of broadcasting sowing, is used low light level processing, maximum light intensity is no more than 2000 luxs, adds gradually intense light irradiation after sprouting, but the highest 20000 luxs that are no more than;
Step 9, water, fertilizer, plant disease management: maintain seedbed moisture content 60%～65 ﹪ with sprinkling equipment, air humidity 80 ﹪～90 ﹪, extract out after two leaves Deng seed germination, spray weekly a flower foliage fertilizer of 1 to 2 time 1 ‰, daily disease by within every ten days, spray one time 600 times carbendazim or the thiophanate methyl of 750 times;
Step 10, transplant: in the spring of Second Year, by possessing complete, the stem of noble dendrobium seedling of stem, leaf, rise and transplant together with matrix one digging, follow-up management is by normal planting type.
2. the method for the live stem of noble dendrobium seedling of a kind of plastic tunnel as claimed in claim 1, is characterized in that: in described step 1, described greenhouse is high 2.5～4 meters, wide 5～7 meters.
3. the method for the live stem of noble dendrobium seedling of a kind of plastic tunnel as claimed in claim 2, is characterized in that: the sunshade net that covers 6～9 thick plastic foils and sunshade rate 70 ﹪～90% above booth.
4. the method for the live stem of noble dendrobium seedling of a kind of plastic tunnel as claimed in claim 2 or claim 3, is characterized in that: plastic deeping rides on the fixed mount of angle steel welding, and built on stilts with hollow concrete block under angle steel, and seedbed is wide 1～2 meter, and length is as the criterion with canopy length.
5. the method for the live stem of noble dendrobium seedling of a kind of plastic tunnel as claimed in claim 1, is characterized in that: in the time of the live Dendrobidium huoshanness seedling of plastic tunnel, above-mentioned steps 1 to 10 is specially:
Step 1, build booth: greenhouse is high 3 meters, wide 6 meters, covers the sunshade net of 8 thick plastic foils and sunshade rate 90 ﹪ above booth;
Step 2, making seedbed: plastic deeping rides on the fixed mount of angle steel welding, and built on stilts with hollow concrete block under angle steel, seedbed is wide 1.5 meters, and length is as the criterion with canopy length;
Step 3, matrix and sterilization: pine bark and sheep excrement were mixed by 4: 1, after the corruption thoroughly of fermenting, cross 4 object screen clothes, with the carbendazim immersions of 600 times 4 minutes, then pave on seedbed, 10 centimetres of thickness, place 7 days, and control moisture content is at 60 ﹪;
Step 4, fungal component inoculation: get 2 years above old matrix of the cultivation stem of noble dendrobium, cross 4 object screen clothes, spread the consumption of 1 kilogram of old matrix by every square metre, old matrix is sprinkling upon uniformly on the seedbed of completing new matrix;
Step 5, seed pretreatment: by the alcohol-pickled sterilization of 75 ﹪ 5 minutes for ripe stem of noble dendrobium capsule, then cut open, the seed in fruit is poured in the sterile water that contains 2 mg/litre methyl α-naphthyl acetates and 0.5 mg/litre 6-benzyl aminopurine and infiltrates 5 hours;
Sterilization in step 6, canopy: the guarantor mushroom king that every cubic metre of use is 4 grams is stifling, before sowing stifling once, later stifling once every two weeks;
Step 7, broadcast sowing: start sowing the spring turning after warm at outside air temperature, is also outdoor temperature 17～25 degree, first on seedbed, gets 1 kilogram of matrix, mix 10 seeds that soaked hormone, after then mixing thoroughly, be sprinkling upon on seedbed, area is 10 square metres;
Step 8, temperature, light, humidity control: after broadcasting sowing, to transplanting, temperature of shed is controlled at 28 DEG C, and seedbed moisture control is at 60 ﹪, and air humidity maintains 80 ﹪; The first month of broadcasting sowing, is used low light level processing, and maximum intensity is no more than 2000 luxs, strengthens gradually illumination after sprouting, but the highest 20000 luxs that are no more than;
Step 9, water, fertilizer, plant disease management: maintain seedbed moisture content 60 ﹪ with sprinkling equipment, air humidity 80 ﹪, extract out after two leaves Deng Dendrobidium huoshanness, spray weekly a flower foliage fertilizer of 1 time 1 ‰, daily disease is by within every ten days, spraying the carbendazim of 600 times one time;
Step 10, transplanting: in the spring of Second Year, by possessing the stem of noble dendrobium seedling of complete root, stem, leaf, rise together with matrix one digging, transplant by the seeding row spacing of 10 centimetres of 10 cm x, follow-up management is by normal planting type.
6. the method for the live stem of noble dendrobium seedling of a kind of plastic tunnel as claimed in claim 1, is characterized in that: in the time of the live HERBA DENDROBII seedling of plastic tunnel, above-mentioned steps 1 to 10 is specially:
Step 1, build booth: described greenhouse is high 2.5 meters, wide 5 meters, covers the sunshade net of 6 thick plastic foils and sunshade rate 70 ﹪ above booth;
Step 2, making seedbed: plastic deeping rides on the fixed mount of angle steel welding, and built on stilts with hollow concrete block under angle steel, seedbed is wide 1 meter, and length is as the criterion with canopy length;
Step 3, matrix and sterilization: pine bark and sheep excrement were mixed by 3: 1, after the corruption thoroughly of fermenting, cross 3 object screen clothes, with the carbendazim immersions of 600 times 5 minutes, then pave on seedbed, 8 centimetres of thickness, place 5 days, and controlled humidity is at 65 ﹪;
Step 4, fungal component inoculation: get 2 years above old matrix of the cultivation stem of noble dendrobium, cross 3 object screen clothes, spread the consumption of 1.2 kilograms of old matrix by every square metre, old matrix is sprinkling upon uniformly on the seedbed of completing new matrix;
Step 5, seed pretreatment: by the alcohol-pickled sterilization of 75 ﹪ 4 minutes for ripe stem of noble dendrobium capsule, then cut open, the seed in fruit is poured in the sterile water that contains 2 mg/litre methyl α-naphthyl acetates and 0.5 mg/litre 6-benzyl aminopurine and infiltrates 4 hours;
Sterilization in step 6, canopy: the guarantor mushroom king that every cubic metre of use is 4 grams is stifling, before sowing stifling once, later stifling once every two weeks;
Step 7, broadcast sowing: start sowing the spring turning after warm at outside air temperature, is also outdoor temperature 17～25 degree, first on seedbed, gets 0.8 kilogram of matrix, mixes 8 seeds that soaked hormone, after then mixing thoroughly, is sprinkling upon on seedbed, and area is 8 square metres;
Step 8, temperature, light, humidity control: after broadcasting sowing, to transplanting, temperature of shed is controlled at 18 DEG C, and seedbed humidity is controlled at 65%, and air humidity maintains 70 ﹪; The first month of broadcasting sowing, is used low light level processing, and maximum intensity is no more than 2000 luxs, strengthens gradually illumination after sprouting, but the highest 20000 luxs that are no more than;
Step 9, water, fertilizer, plant disease management: maintain seedbed humidity 65% with sprinkling equipment, air humidity 70 ﹪, wait the stem of noble dendrobium to extract out after two leaves, spray weekly a flower foliage fertilizer of 1 time 1 ‰; Daily disease is by within every ten days, spraying the thiophanate methyl of 750 times one time;
Step 10, transplanting: in the spring of Second Year, by possessing the stem of noble dendrobium seedling of complete root, stem, leaf, rise together with matrix one digging, transplant by the seeding row spacing of 12 centimetres of 8 cm x, follow-up management is by existing planting type.
7. the method for the live stem of noble dendrobium seedling of a kind of plastic tunnel as claimed in claim 1, is characterized in that: in the time of the live dendrobium candidum seedling of plastic tunnel, above-mentioned steps 1 to 10 is specially:
Step 1, build booth: described greenhouse is high 4 meters, wide 7 meters, covers the sunshade net of 9 thick plastic foils and sunshade rate 90 ﹪ above booth;
Step 2, making seedbed: plastic deeping rides on the fixed mount of angle steel welding, and built on stilts with hollow concrete block under angle steel, seedbed is wide 2 meters, and length is as the criterion with canopy length;
Step 3, matrix and sterilization: pine bark and sheep excrement were mixed by 5: 1, after the corruption thoroughly of fermenting, cross 5 object screen clothes, with the carbendazim immersions of 600 times 3 minutes, then pave on seedbed, 12 centimetres of thickness, place 10 days, and controlled humidity is at 62 ﹪;
Step 4, fungal component inoculation: get 2 years above old matrix of the cultivation stem of noble dendrobium, cross 5 object screen clothes, spread the consumption of 1.5 kilograms of old matrix by every square metre, old matrix is sprinkling upon uniformly on the seedbed of completing new matrix;
Step 5, seed pretreatment: by the alcohol-pickled sterilization of 75 ﹪ 6 minutes for ripe stem of noble dendrobium capsule, then cut open, the seed in fruit is poured in the sterile water that contains 2 mg/litre methyl α-naphthyl acetates and 0.5 mg/litre 6-benzyl aminopurine and infiltrates 5 hours;
Sterilization in step 6, canopy: the guarantor mushroom king that every cubic metre of use is 4 grams is stifling, before sowing stifling once, later stifling once every two weeks;
Step 7, broadcast sowing: start sowing the spring turning after warm at outside air temperature, is also outdoor temperature 17～25 degree, first on seedbed, gets 1.2 kilograms of matrix, mixes 12 seeds that soaked hormone, after then mixing thoroughly, is sprinkling upon on seedbed, and area is 12 square metres;
Step 8, temperature, light, humidity control: after broadcasting sowing, to transplanting, temperature of shed is controlled at 25 DEG C, and seedbed humidity is controlled at 62%, and air humidity maintains 80 ﹪; The first month of broadcasting sowing, is used low light level processing, and maximum intensity is no more than 2000 luxs, strengthens gradually illumination after sprouting, but the highest 20000 luxs that are no more than;
Step 9, water, fertilizer, plant disease management: maintain seedbed humidity 62% with sprinkling equipment, air humidity 80 ﹪, wait the stem of noble dendrobium to extract out after two leaves, spray weekly a flower foliage fertilizer of 2 times 1 ‰; Daily disease is by within every ten days, spraying the thiophanate methyl of 750 times one time;
Step 10, transplanting: in the spring of Second Year, by possessing the stem of noble dendrobium seedling of complete root, stem, leaf, rise together with matrix one digging, transplant by the seeding row spacing of 17 centimetres of 12 cm x, follow-up management is by existing planting type.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310004397.7A CN103004445B (en) | 2013-01-06 | 2013-01-06 | Method for directly seedling dendrobe seedlings by using plastic greenhouse |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310004397.7A CN103004445B (en) | 2013-01-06 | 2013-01-06 | Method for directly seedling dendrobe seedlings by using plastic greenhouse |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103004445A CN103004445A (en) | 2013-04-03 |
| CN103004445B true CN103004445B (en) | 2014-07-23 |
Family
ID=47953976
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310004397.7A Active CN103004445B (en) | 2013-01-06 | 2013-01-06 | Method for directly seedling dendrobe seedlings by using plastic greenhouse |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103004445B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108293863A (en) * | 2018-02-11 | 2018-07-20 | 绍兴儒林生物科技有限公司 | A kind of dendrobium candidum ecosystem is from the implantation methods that germinate |
Families Citing this family (24)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103098691B (en) * | 2013-01-18 | 2015-09-09 | 安徽同济生生物科技有限公司 | Dendrobium huoshanense pollution-free imitating wild planting process |
| CN103210781A (en) * | 2013-05-14 | 2013-07-24 | 贵州省赤水市金钗石斛产业开发有限公司 | Dendrobium nobile stone cultivating method |
| CN103250620A (en) * | 2013-05-15 | 2013-08-21 | 赤水市华宝石斛产业有限公司 | Dendrobium nobile standardized facility cultivating method |
| CN103477965B (en) * | 2013-09-10 | 2015-08-19 | 江苏农林职业技术学院 | A kind of cultivation seedbed, ground of dendrobium candidum |
| CN103798013B (en) * | 2013-12-23 | 2016-01-27 | 茂名市惠生源生物科技有限公司 | A kind of breeding method of Seeds of Dendrobium Candidum seedling |
| CN103782886B (en) * | 2014-02-13 | 2015-12-30 | 云南新维特生物科技有限公司 | A kind of greenhouse gardening method of dendrobium candidum and application |
| CN103918471B (en) * | 2014-05-13 | 2015-08-12 | 赤水市信天中药产业开发有限公司 | HERBA DENDROBII seed booth sprouts seedling raising process |
| CN104106453A (en) * | 2014-06-28 | 2014-10-22 | 赵木华 | Method for constructing materials for planting dendrobium officinale on planes |
| CN104145795B (en) * | 2014-08-15 | 2016-08-24 | 湖南省农业信息与工程研究所 | A kind of dendrobe cultivation method improving facility utilization rate |
| CN104488674A (en) * | 2014-12-03 | 2015-04-08 | 柳州市泓吉农业科技有限公司 | Field cultivation method for dendrobium |
| CN104770278B (en) * | 2015-04-03 | 2017-08-25 | 广西经正科技开发有限责任公司 | A kind of dendrobium candidum greenhouse intensive cultivating method |
| CN105875138A (en) * | 2015-04-29 | 2016-08-24 | 广西桂平市仙宝园铁皮石斛有限责任公司 | Method for breeding direct seeding seedlings through natural seeding of dendrobe seeds |
| CN106332757A (en) * | 2016-08-25 | 2017-01-18 | 赵孝巍 | Dendrobe seedling culture technology |
| CN106688845A (en) * | 2016-11-23 | 2017-05-24 | 戴亚峰 | Dendrobium huoshanense seedling domestication method |
| CN106900316B (en) * | 2017-02-06 | 2020-04-07 | 云南农业大学 | Method for natural propagation of wild large-flower flax grass seeds |
| CN107041285A (en) * | 2017-02-07 | 2017-08-15 | 高久双 | A kind of method of the live seedling of Dendrobidium huoshanness ball stem |
| CN107046989A (en) * | 2017-03-21 | 2017-08-18 | 农凯峰 | The cultural method of dendrobium candidum |
| CN107046988A (en) * | 2017-03-21 | 2017-08-18 | 农凯峰 | The green cultural method of dendrobium candidum |
| CN107079679A (en) * | 2017-03-21 | 2017-08-22 | 农凯峰 | The high-yield planting method of dendrobium candidum |
| CN107046987A (en) * | 2017-03-21 | 2017-08-18 | 农凯峰 | The implantation methods of dendrobium candidum |
| CN108834889B (en) * | 2018-05-31 | 2021-11-30 | 贵州盛达生植物发展有限公司 | Tissue culture seedling cultivation method for improving disease resistance of dendrobium officinale |
| CN111386979A (en) * | 2020-03-26 | 2020-07-10 | 陕西锶长久中药材种植科技有限公司 | Seedling growing method of rhizoma atractylodis macrocephalae |
| CN111919749B (en) * | 2020-08-20 | 2022-03-25 | 南京农业大学 | Culture medium for rapid propagation of dendrobium nobile seedlings and rapid propagation method thereof |
| CN114375774A (en) * | 2022-01-12 | 2022-04-22 | 张正军 | High-survival-rate propagation method for dendrobium nobile |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1618270A (en) * | 2003-11-19 | 2005-05-25 | 张云 | Method for quick naturalization of medicinal dendrobe test-tube plantlet |
| CN1324946C (en) * | 2004-11-24 | 2007-07-11 | 上海增靓生物科技有限公司 | Large-area cultivation of officinal dendrobium |
| CN1849865A (en) * | 2006-05-22 | 2006-10-25 | 腾冲县晨光林业有限责任公司 | Method for planting medicinal dendrobium by using elevated tank and base material |
| CN101889547B (en) * | 2009-05-22 | 2012-09-26 | 云南省德宏热带农业科学研究所 | Aseptic and rapid propagation method of dendrobium devonianum seeds |
| CN101695272B (en) * | 2009-10-26 | 2011-03-30 | 重庆市中药研究院 | Natural reproduction method for dendrobium |
| CN101779558B (en) * | 2010-02-04 | 2011-08-03 | 龙陵县石斛协会 | Planting method of purple dendrobium with meshed membrane frame bed crossbar |
| CN101999307B (en) * | 2010-10-21 | 2013-07-03 | 重庆市中药研究院 | Natural reproduction method for sowing dendrobium |
| CN102318547B (en) * | 2011-07-22 | 2013-07-31 | 安徽康顺名贵中草药产业开发有限公司 | Overhead cultivation method of Dendrobium huoshanense test-tube seedlings |
| CN102440130A (en) * | 2011-09-22 | 2012-05-09 | 澄思源生物科技（上海）有限公司 | Method for natural propagation of dendrobium officinale seeds |
| CN102613056B (en) * | 2012-04-18 | 2015-07-15 | 浙江农林大学 | Original ecological culture method of dendrobium candidum |
-
2013
- 2013-01-06 CN CN201310004397.7A patent/CN103004445B/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108293863A (en) * | 2018-02-11 | 2018-07-20 | 绍兴儒林生物科技有限公司 | A kind of dendrobium candidum ecosystem is from the implantation methods that germinate |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103004445A (en) | 2013-04-03 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103004445B (en) | Method for directly seedling dendrobe seedlings by using plastic greenhouse | |
| CN102356746B (en) | Technology for cultivating organic fruit and vegetable matrix | |
| CN102550252B (en) | Cultivation method of Dendrobium officinale | |
| CN105248242B (en) | A kind of breeding method of early-ripening grape | |
| CN102939843B (en) | Method for domesticating hardening seedlings of tissue culture seedlings of Nandina domestica firepower | |
| CN105230333B (en) | A kind of safflower-rice, tree peony and green vegetables crop rotation implantation methods | |
| CN104126397A (en) | Bletilla direct seeding seedling culture method | |
| CN104025987A (en) | Dendrobium officinale planting method | |
| CN104628445A (en) | Special fertilizer and foliage fertilizer for dendrobium nobile and planting method of dendrobium nobile | |
| CN103355027A (en) | Taxus chinensis var.mairei seedling breeding method | |
| CN105475076A (en) | Ligustrun lucidum ait cutting and cultivating method | |
| CN105532471A (en) | Novel high-yield, high-quality and large-scale polygonatum cyrtonema cultivation method | |
| CN107801619A (en) | A kind of implantation methods of greenhouse cherry and tomato | |
| CN104145795A (en) | Dendrobe cultivation method for improving facility utilization rate | |
| CN102960226A (en) | Dendrobium officinale Kimura et Migo tissue culture seedling acclimatization method | |
| CN104521705A (en) | Cultivating and conserving method for facility strawberries | |
| CN103340076B (en) | Bulbus fritillariae cirrhosae greenhouse cultivation method | |
| CN102960223B (en) | Method for simply, easily and quickly cultivating tobacco seedlings | |
| CN104718946A (en) | Year-round cultivation method for sword-shaped sharp peppers | |
| CN107371691A (en) | The cultural method of organic Se-rich, high sugared dendrobium candidum | |
| CN105557280A (en) | Method for managing environmental factors during Chinese onion seedling culture | |
| CN104604465A (en) | Planting method for aloe | |
| CN106665047A (en) | Cultivation method for greenhouse muskmelons | |
| CN106561204A (en) | Method for improving alcohol-soluble extractive content of dendrobium officinale kimura et migo | |
| CN106489503A (en) | Oil tree peony Clonal regeneration method for culturing seedlings |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |