CN103098711A - Rooting and transplanting method for dendrobium officinale tissue culture - Google Patents

Rooting and transplanting method for dendrobium officinale tissue culture Download PDF

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Publication number
CN103098711A
CN103098711A CN2013100315755A CN201310031575A CN103098711A CN 103098711 A CN103098711 A CN 103098711A CN 2013100315755 A CN2013100315755 A CN 2013100315755A CN 201310031575 A CN201310031575 A CN 201310031575A CN 103098711 A CN103098711 A CN 103098711A
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root
days
hardening
transplanting
sphagna
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CN2013100315755A
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彭玉辅
彭火辉
管帮富
陈华玲
彭华
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JIANGXI KING TREE GARDEN CO Ltd
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JIANGXI KING TREE GARDEN CO Ltd
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    • Y02P60/216

Abstract

The invention discloses a rooting and transplanting method for dendrobium officinale tissue culture. The method comprises technical processes of rooting culture, seedling hardening-off and transplanting. The prescription of culture mediums of different culture stages, the culture condition and the composition and proportion of a transplanting matrix are uniquely innovated through strict multiple comparison tests, the survival rate of sphagna hardening-off is 95%, and the survival rate of matrix transplantation is 97%. Establishment of a rapid dendrobium officinale propagation technique system by using the method disclosed by the invention to perform artificial propagation can accelerate the propagation of the dendrobium officinale, the survival rate of the dendrobium officinale can be greatly increased by the sphagna hardening-off stage, and the cultured dendrobium officinale can be used as an excellent medicine material.

Description

Dendrobium candidum tissue is cultivated and is taken root and transplanting method
Technical field
The present invention relates to a kind of dendrobium candidum Plant Tissue Breeding fast breeding technique, relate in particular to a kind of dendrobium candidum tissue cultivation and take root and transplanting method.
Background technology
Dendrobium candidum ( Dendrobium officinale Kimura et Migo) be under the jurisdiction of the orchid family ( Orchidaceae), Dendrobium ( Dendrobium Sw.) the perennial herbaceous plant that grows nonparasitically upon another plant.Wild dendrobium candidum great majority are distributed in the countries and regions such as East Asia, Southeast Asia and Australia.The wild dendrobium candidum great majority of China are distributed in the mountain area on the ground such as the Qinling Mountains, the Anhui on the south Huaihe River, Zhejiang, Jiangxi, cloud, expensive, river; in the rock crevice of the overhanging cliff that grows in mainly that lofty mountains and steep hills; wild dendrobium candidum resource has approached exhaustion now, is the rare or endangered species of state key second class protection.Dendrobium candidum contains the healthy medicinal active ingredient that is beneficial to man in a large number, has unique medical value, has clearind deficient heat, nourishing Tianjin, tonifying five zang organs consumptive disease, improves immunity and the anti-ageing effect of waiting for a long time.Have a wide range of applications in fields such as disease treatment and prevention from suffering from the diseases, nutrition and health care and beautifying face and moistering lotions in recent years.
Summary of the invention
The object of the present invention is to provide a kind of dendrobium candidum tissue to cultivate takes root and transplanting method, the method utilizes the method for sphagna hardening to promote the stalwartness of taking root, promote the dendrobium candidum seedling of dendrobium candidum seedling, greatly improve the survival rate of dendrobium candidum seedling, can shorten the growth cycle of dendrobium candidum simultaneously.
Technical scheme of the present invention is: a kind of dendrobium candidum tissue is cultivated and is taken root and transplanting method, its step: culture of rootage: the Multiple Buds seedling is inoculated into root induction on the bottled root media of film sealing; Hardening and transplanting: after cultivating 28-30 days on root media, removed the spacious bottle of sealed membrane hardening 4-5 days, taking-up group training seedling, take every clump of 5 about strains as unit, after cleaning the medium on root and carrying out disinfection, the root system that to organize clockwise the training seedling with the sphagna of sterilization wraps, and is transplanted to cultivation in 96 hole dishes.After hardening to 2 month, be transplanted in the matrix that fills sterilization and cultivate in sphagna.
In described root media step: the formula of described root media is: MS medium+sucrose 30g/L+ agar 8g/L+IBA0.4mg/L+ murphy juice 100g/L, PH5.8-6.0.
In described hardening and transplant step: the root sterilization method to group training seedling is: dip 5-10s in 0.5% mancozeb solution; To the sphagna sterilization method be: be 120 ℃ in temperature, with high steam pot sterilization 20 minutes; The formula of described matrix is: pine bark: wood chip=4.5:1(volume ratio), described matrix is with 0.5% mancozeb solution and 0.8% Isofenphos methyl soaking disinfection; The hardening condition is: keep 23-27 ℃ of temperature, and air humidity 78-85%, fine day watered 1 time every 4 days, and the rainy day watered 1 time in 9-11 days, every less important sphagna that irrigates, after 2 months, shoot root is flourishing.Cultivation condition is: maintain the temperature at 23-27 ℃, and air humidity 73-78%, fine day watered 1 time every 2-3 days, and the rainy day watered 1 time in 7-9 days, and every less important matrix that irrigates survived in 15-17 days.
The MS culture medium prescription of root media comprises macroelement, trace element, molysite and organic matter,
The MS culture medium prescription:
The invention has the advantages that: dendrobium candidum tissue is cultivated and is taken root and transplanting method, comprises the sport technique segments such as culture of rootage, hardening and transplanting.Than experimental study, from the composition of formula, condition of culture and the transplanting medium of the medium of different cultivation stages and proportioning etc., original innovation is arranged by strict multiple ratio, sphagna hardening survival rate is 95%, and the substrate culture survival rate is 97%.Use the present invention and set up the rapid propagation of Dendrobium candidum technical system, carry out artificial propagation, the expansion that can accelerate dendrobium candidum is numerous, can be used as good medicinal material.
Embodiment
Dendrobium candidum tissue of the present invention is cultivated and is taken root and transplanting method, and its better embodiment comprises step:
Culture of rootage: the Multiple Buds seedling is inoculated into root induction on the bottled root media of film sealing;
Hardening and transplanting: cultivated 28-30 days on root media, removed the spacious bottle of sealed membrane hardening 4-5 days, taking-up group training seedling.Take every clump of 4-5 strain as unit, after cleaning the medium on root and carrying out disinfection, the root system that will organize clockwise the training seedling with the sphagna of sterilization wraps, and is transplanted to 96 holes and cultivates in coiling.After hardening to 2 month, be transplanted in the matrix that fills sterilization and cultivate in sphagna.
In described culture of rootage step: the formula of described root media is: MS medium+sucrose 30g/L+ agar 8g/L+IBA(indolebutyric acid) 0.4mg/L+ murphy juice 100g/L, PH5.8-6.0.
In described hardening and transplant step:
To the sterilization method of the root of group training seedling can for: dip 5-10s in 0.5% mancozeb solution;
To the sterilization method of sphagna can for: be 120 ℃ in temperature, with high steam pot sterilization 20 minutes;
Described matrix formulations can be pine bark: wood chip=4.5:1(volume ratio), described matrix is with 0.5% mancozeb solution and the different sulphur phosphorus of 0.8% methyl soaking disinfection;
The hardening condition is: maintain the temperature at 23-27 ℃, and air humidity 78-85%, fine day watered 1 time every 4 days, and the rainy day watered 1 time in 9-11 days, every less important sphagna that irrigates, after 2 months, shoot root is flourishing.
Cultivation condition is: maintain the temperature at 23-27 ℃, about air humidity 73-78%, fine day watered 1 time every 2 to 3 days, and the rainy day watered 1 time in 7-9 days, and every less important matrix that irrigates survived in 15-17 days.
The dendrobium candidum that carries out artificial propagation by method of the present invention can be used as medicinal plant.
Specific embodiment:
Culture of rootage: be the seedling of 5-6cm with height, be inoculated into root induction on root media, the formula of medium is: MS+ sucrose 30g/L+ agar 8g/L+IBA(indolebutyric acid) 0.4mg/L+ murphy juice 100g/L, PH5.8-6.0, begin to take root after 5 days, rooting rate reaches 100%.
Hardening and transplanting: cultivated 30 days on root media, removed the spacious bottle of sealed membrane hardening 4-5 days, taking-up group training seedling.Take clump as unit (about every clump of 5 strains), clean the medium on root, dip 5-10s in 0.5% mancozeb solution, be transplanted in the 96 hole dishes that fill the sphagna that crosses with high pressure steam sterilization, maintain the temperature at 23-27 ℃, air humidity 78-85%, fine day watered 1 time every 4 days, and the rainy day watered 1 time in 9-11 days, every less important sphagna that irrigates, after 2 months, shoot root is flourishing, survival rate 95%.Then will refine good transplantation of seedlings in the pallet that fills with the pine bark of 0.5% mancozeb solution and the different sulphur phosphorus of 0.8% methyl soaking disinfection and wood chip matrix (pine bark is 4.5 to 1 with the volume ratio of wood chip), maintain the temperature at 23-27 ℃, air humidity 73-78%, fine day watered 1 time every 2 to 3 days, rainy day watered 1 time in 7-9 days, every less important matrix that irrigates survived survival rate 97% in 15-17 days.
Dendrobium candidum tissue of the present invention is cultivated and is taken root and transplanting method, comprises the sport technique segments such as culture of rootage, hardening and transplanting.Than experimental study, from the composition of formula, condition of culture and the transplanting medium of the medium of different cultivation stages and proportioning etc., original innovation is arranged by strict multiple ratio, sphagna hardening survival rate can be 95%, and the matrix transplanting survival rate is 97%.Use the present invention and set up the rapid propagation of Dendrobium candidum technical system, carry out artificial propagation, the expansion that can accelerate dendrobium candidum is numerous, can be used as good medicinal material.
The above; only be the better specific implementation method of the present invention, but protection scope of the present invention is not limited to this, any those of ordinary skill in the art are in the technical scope that the present invention discloses; the variation that can expect easily or replacement are within all should being encompassed in protection scope of the present invention.Therefore, protection scope of the present invention should be as the criterion with the protection domain of claims.

Claims (4)

1. a dendrobium candidum tissue is cultivated and is taken root and transplanting method, its step:
Culture of rootage: the Multiple Buds seedling is inoculated into root induction on the bottled root media of film sealing;
Hardening and transplanting: after cultivating 28-30 days on root media, removed the spacious bottle of sealed membrane hardening 4-5 days, taking-up group training seedling, take every clump of 5 about strains as unit, after cleaning the medium on root and carrying out disinfection, the root system that will organize clockwise the training seedling with the sphagna of sterilization wraps, and is transplanted to 96 holes and cultivates in coiling, after hardening to 2 month, be transplanted in the matrix that fills sterilization and cultivate in sphagna.
2. dendrobium candidum tissue according to claim 1 is cultivated and is taken root and transplanting method, it is characterized in that: in described root media step: the formula of described root media is: MS medium+sucrose 30g/L+ agar 8g/L+IBA0.4mg/L+ murphy juice 100g/L, PH5.8-6.0.
3. dendrobium candidum tissue according to claim 1 is cultivated and is taken root and transplanting method, it is characterized in that: in described hardening and transplant step:
Root sterilization method to group training seedling is: dip 5-10s in 0.5% mancozeb solution;
To the sphagna sterilization method be: be 120 ℃ in temperature, with high steam pot sterilization 20 minutes;
The formula of described matrix is: pine bark: wood chip=4.5:1(volume ratio), described matrix is with 0.5% mancozeb solution and 0.8% Isofenphos methyl soaking disinfection;
The hardening condition is: keep 23-27 ℃ of temperature, and air humidity 78-85%, fine day watered 1 time every 4 days, and the rainy day watered 1 time in 9-11 days, every less important sphagna that irrigates, after 2 months, shoot root is flourishing;
Cultivation condition is: maintain the temperature at 23-27 ℃, and air humidity 73-78%, fine day watered 1 time every 2-3 days, and the rainy day watered 1 time in 7-9 days, and every less important matrix that irrigates survived in 15-17 days.
4. dendrobium candidum tissue according to claims 1 or 2 cultivated and taken root and transplanting method, and it is characterized in that: the MS culture medium prescription of root media comprises macroelement, trace element, molysite and organic matter,
The MS culture medium prescription:
Figure 2013100315755100001DEST_PATH_IMAGE001
CN2013100315755A 2013-01-28 2013-01-28 Rooting and transplanting method for dendrobium officinale tissue culture Pending CN103098711A (en)

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Cited By (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103355167A (en) * 2013-07-17 2013-10-23 红河州巨丰生物科技有限公司 Dendrobium officinale tissue culture method with plastic milk tea cup as tissue culture container
CN103918558A (en) * 2014-05-04 2014-07-16 中国热带农业科学院橡胶研究所 Method for domesticating dendrobium officinale kimuraet migo tissue culture seedling by using glass bottle
CN104094851A (en) * 2014-07-23 2014-10-15 上海农业科技种子有限公司 Dendrobium candidum test-tube seedling rooting medium and preparation method thereof
CN104145795A (en) * 2014-08-15 2014-11-19 湖南省农业信息与工程研究所 Dendrobe cultivation method for improving facility utilization rate
CN104488706A (en) * 2014-12-03 2015-04-08 柳州市泓吉农业科技有限公司 Dendrobe officinale transplanting method
CN105145298A (en) * 2015-07-30 2015-12-16 黄旭胜 Dendrobium officinale pot culturing method
CN105557486A (en) * 2015-12-17 2016-05-11 福建连天福生物科技有限公司 Preparation method of light media for dendrobium officinale
CN105830893A (en) * 2016-05-26 2016-08-10 霍山宝信园石斛开发有限公司 Raw materials and method for planting Dendrobium candidum
CN106171888A (en) * 2016-07-18 2016-12-07 福建连天福生物科技有限公司 A kind of dendrobium officinale culture medium and cultural method
CN106962165A (en) * 2017-05-22 2017-07-21 东莞市睿绅生物技术有限公司 A kind of preparation method of moveable dendrobium officinale culture medium and integrated method for culturing seedlings
CN107182734A (en) * 2017-04-27 2017-09-22 江西九草铁皮石斛科技协同创新有限公司 A kind of dendrobium candidum supports method with Ranaspinosa David set
CN107691126A (en) * 2017-09-27 2018-02-16 霍山宝信园石斛开发有限公司 A kind of method for growing dendrobium

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CN101273709A (en) * 2008-05-14 2008-10-01 南京养颐堂生物科技实业有限公司 Tissue culture method for rapid propagation of Dendrobium candidum
KR100868523B1 (en) * 2007-01-25 2008-11-12 김종선 Mass propagation method through in vitro aseptic germination of Calanthe spp.
CN102144541A (en) * 2010-12-31 2011-08-10 东莞市睿绅生物技术有限公司 Three-step method for producing 'dragon head phoenix tail' high-quality officinal dendrobium stem material
CN102217551A (en) * 2011-06-14 2011-10-19 广东省农业科学院花卉研究所 Tissue culture quick reproduction method for dendrobium chrysotoxum lindl bud tips
CN102763596A (en) * 2012-08-08 2012-11-07 广东省林业科学研究院 High-efficiency quick dendrobium candidum tissue culture and propagation method

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KR100868523B1 (en) * 2007-01-25 2008-11-12 김종선 Mass propagation method through in vitro aseptic germination of Calanthe spp.
CN101273709A (en) * 2008-05-14 2008-10-01 南京养颐堂生物科技实业有限公司 Tissue culture method for rapid propagation of Dendrobium candidum
CN102144541A (en) * 2010-12-31 2011-08-10 东莞市睿绅生物技术有限公司 Three-step method for producing 'dragon head phoenix tail' high-quality officinal dendrobium stem material
CN102217551A (en) * 2011-06-14 2011-10-19 广东省农业科学院花卉研究所 Tissue culture quick reproduction method for dendrobium chrysotoxum lindl bud tips
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Cited By (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103355167A (en) * 2013-07-17 2013-10-23 红河州巨丰生物科技有限公司 Dendrobium officinale tissue culture method with plastic milk tea cup as tissue culture container
CN103918558B (en) * 2014-05-04 2016-03-23 中国热带农业科学院橡胶研究所 A kind of method of candidum tissue culturing seedling glass bottle domestication
CN103918558A (en) * 2014-05-04 2014-07-16 中国热带农业科学院橡胶研究所 Method for domesticating dendrobium officinale kimuraet migo tissue culture seedling by using glass bottle
CN104094851A (en) * 2014-07-23 2014-10-15 上海农业科技种子有限公司 Dendrobium candidum test-tube seedling rooting medium and preparation method thereof
CN104145795A (en) * 2014-08-15 2014-11-19 湖南省农业信息与工程研究所 Dendrobe cultivation method for improving facility utilization rate
CN104145795B (en) * 2014-08-15 2016-08-24 湖南省农业信息与工程研究所 A kind of dendrobe cultivation method improving facility utilization rate
CN104488706A (en) * 2014-12-03 2015-04-08 柳州市泓吉农业科技有限公司 Dendrobe officinale transplanting method
CN105145298A (en) * 2015-07-30 2015-12-16 黄旭胜 Dendrobium officinale pot culturing method
CN105557486A (en) * 2015-12-17 2016-05-11 福建连天福生物科技有限公司 Preparation method of light media for dendrobium officinale
CN105830893A (en) * 2016-05-26 2016-08-10 霍山宝信园石斛开发有限公司 Raw materials and method for planting Dendrobium candidum
CN106171888A (en) * 2016-07-18 2016-12-07 福建连天福生物科技有限公司 A kind of dendrobium officinale culture medium and cultural method
CN107182734A (en) * 2017-04-27 2017-09-22 江西九草铁皮石斛科技协同创新有限公司 A kind of dendrobium candidum supports method with Ranaspinosa David set
CN106962165A (en) * 2017-05-22 2017-07-21 东莞市睿绅生物技术有限公司 A kind of preparation method of moveable dendrobium officinale culture medium and integrated method for culturing seedlings
CN107691126A (en) * 2017-09-27 2018-02-16 霍山宝信园石斛开发有限公司 A kind of method for growing dendrobium

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Application publication date: 20130515