CN105340708B - A kind of Radix Notoginseng factorial seedling-culturing method - Google Patents
A kind of Radix Notoginseng factorial seedling-culturing method Download PDFInfo
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- CN105340708B CN105340708B CN201510759431.0A CN201510759431A CN105340708B CN 105340708 B CN105340708 B CN 105340708B CN 201510759431 A CN201510759431 A CN 201510759431A CN 105340708 B CN105340708 B CN 105340708B
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- Prior art keywords
- seedling
- radix notoginseng
- floating
- culturing method
- seed
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- 241000180649 Panax notoginseng Species 0.000 title claims abstract description 35
- 235000003143 Panax notoginseng Nutrition 0.000 title claims abstract description 32
- 238000000034 method Methods 0.000 title claims abstract description 22
- 238000012258 culturing Methods 0.000 title claims abstract description 10
- 238000007667 floating Methods 0.000 claims abstract description 21
- 201000010099 disease Diseases 0.000 claims abstract description 12
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims abstract description 12
- 239000010451 perlite Substances 0.000 claims abstract description 9
- 235000019362 perlite Nutrition 0.000 claims abstract description 9
- 239000002689 soil Substances 0.000 claims description 11
- 239000000243 solution Substances 0.000 claims description 10
- FGIUAXJPYTZDNR-UHFFFAOYSA-N potassium nitrate Chemical compound [K+].[O-][N+]([O-])=O FGIUAXJPYTZDNR-UHFFFAOYSA-N 0.000 claims description 8
- 238000005507 spraying Methods 0.000 claims description 8
- 239000007864 aqueous solution Substances 0.000 claims description 6
- 239000007788 liquid Substances 0.000 claims description 6
- 235000015097 nutrients Nutrition 0.000 claims description 6
- 238000004659 sterilization and disinfection Methods 0.000 claims description 6
- 239000011159 matrix material Substances 0.000 claims description 5
- 239000003415 peat Substances 0.000 claims description 5
- 239000010455 vermiculite Substances 0.000 claims description 5
- 235000019354 vermiculite Nutrition 0.000 claims description 5
- 229910052902 vermiculite Inorganic materials 0.000 claims description 5
- 235000013399 edible fruits Nutrition 0.000 claims description 4
- 235000010333 potassium nitrate Nutrition 0.000 claims description 4
- 239000004323 potassium nitrate Substances 0.000 claims description 4
- 241000607479 Yersinia pestis Species 0.000 claims description 3
- 239000003337 fertilizer Substances 0.000 claims description 3
- 230000006698 induction Effects 0.000 claims description 3
- 239000012286 potassium permanganate Substances 0.000 claims description 3
- 238000007654 immersion Methods 0.000 claims description 2
- 230000001954 sterilising effect Effects 0.000 claims description 2
- 239000000758 substrate Substances 0.000 claims description 2
- 244000131316 Panax pseudoginseng Species 0.000 abstract description 6
- 235000003181 Panax pseudoginseng Nutrition 0.000 abstract description 5
- 239000003375 plant hormone Substances 0.000 abstract description 3
- 230000037452 priming Effects 0.000 abstract description 3
- 238000010923 batch production Methods 0.000 abstract description 2
- 230000000694 effects Effects 0.000 abstract description 2
- 230000035784 germination Effects 0.000 description 5
- 230000001717 pathogenic effect Effects 0.000 description 4
- 239000003814 drug Substances 0.000 description 3
- 238000003306 harvesting Methods 0.000 description 3
- 230000003020 moisturizing effect Effects 0.000 description 3
- 244000052769 pathogen Species 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 230000005070 ripening Effects 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 description 2
- ZCCIPPOKBCJFDN-UHFFFAOYSA-N calcium nitrate Chemical compound [Ca+2].[O-][N+]([O-])=O.[O-][N+]([O-])=O ZCCIPPOKBCJFDN-UHFFFAOYSA-N 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000011419 induction treatment Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 238000004321 preservation Methods 0.000 description 2
- PAWQVTBBRAZDMG-UHFFFAOYSA-N 2-(3-bromo-2-fluorophenyl)acetic acid Chemical class OC(=O)CC1=CC=CC(Br)=C1F PAWQVTBBRAZDMG-UHFFFAOYSA-N 0.000 description 1
- 208000035143 Bacterial infection Diseases 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 239000002202 Polyethylene glycol Substances 0.000 description 1
- 208000022362 bacterial infectious disease Diseases 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 239000006260 foam Substances 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 230000000977 initiatory effect Effects 0.000 description 1
- 230000008774 maternal effect Effects 0.000 description 1
- 238000009335 monocropping Methods 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 229920001223 polyethylene glycol Polymers 0.000 description 1
- 229920006389 polyphenyl polymer Polymers 0.000 description 1
- 229920000915 polyvinyl chloride Polymers 0.000 description 1
- 239000001103 potassium chloride Substances 0.000 description 1
- 235000011164 potassium chloride Nutrition 0.000 description 1
- 238000011012 sanitization Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 230000009469 supplementation Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Soil Sciences (AREA)
- Cultivation Of Plants (AREA)
- Pretreatment Of Seeds And Plants (AREA)
Abstract
The invention discloses a kind of Radix Notoginseng factorial seedling-culturing methods, comprising the following steps: fresh seeds is used, after carrying out vernalization using plant hormone, seed priming is carried out with perlite, it recycles floating seedlings to carry out seedling management, when long to 3-5 centimetres of Radix Notoginseng seedling, can be transplanted.The method of the present invention is easy to operate, can be under the control condition of batch production, the high-quality Radix Notoginseng seedling of high effect culture, while can effectively avoid infecting for soil-borne disease during Radix Notoginseng seedling fostering, reduces the disease during cultivation of pseudo-ginseng and occurs.
Description
Technical field
The invention belongs to drug germchit Cultivating techniques fields, and in particular to a kind of Radix Notoginseng factorial seedling-culturing method.
Background technique
Three seventh is that mostly important one of the Chinese medicine in China, be most suitable for cultural area be Yunnan Province of China mountain of papers partly
Area.Currently used method is seed collecting, preservation, nursery, transplanting, harvesting.Currently used nursery means are moisturizing after harvesting
The seed of preservation is sowed at soil, under conditions of holding temperature appropriate, moisture, sprouts Panax notoginseng seeds, is further formed one
The cultivation of pseudo-ginseng seedling of year life, again by the cultivation of annual cultivation of pseudo-ginseng seedling in new plot, cultivation harvests second year after 2 years
The root tuber of Radix Notoginseng plant is as Chinese medicine Radix Notoginseng.Main problem existing for this method is: firstly, Panax notoginseng seeds have after ripening special
Property, it needs moisturizing to save 3-4 months, is sowed after seed is mature.During moisturizing, compared with vulnerable to pathogenic bacterial infection, make seedling
In spite of illness.Secondly, seed is in the soil in germination process, it is desirable that it is higher, it is easy to be influenced its germination rate, together by such environmental effects
Shi Rongyi is infected by pathogen.Third, seedling in the soil, compared with infecting vulnerable to soil surface characters, make seedling in spite of illness,
Simultaneously because the difference of soil and environment, makes the uniformity of seedling be affected.4th, when seedling Radix Notoginseng is transplanted, root
System is easier to be damaged, and causes the generation of Radix Notoginseng soil-borne disease.
Summary of the invention
For the technical problem present on, the purpose of the present invention is to provide a kind of Radix Notoginseng factorial seedling-culturing methods.
The present invention is realized especially by following technical scheme:
Technical solution of the present invention mainly includes the following contents: using fresh seeds, carries out vernalization using plant hormone, keep away
Exempt from the pathogen contamination during seed after ripening.During presprouting of seeds, seed priming is carried out using perlite, avoids kind
Son is in germination process, since the germination rate that moisture, temperature change are brought is low.Seedling management is carried out using floating seedlings, is reduced
Influence of the seedling stage environment to Radix Notoginseng seedling.Using spraying water management, the moisture in seedling stage is effectively controlled, reduces Radix Notoginseng seedling
Disease incidence.When transplanting, seedling is the cultivation of soil-carried disk, avoids the damage to Radix Notoginseng seedlings root, can reduce soil-borne disease
Occur.
Specifically, a kind of Radix Notoginseng factorial seedling-culturing method, comprising the following steps:
1) the Radix Notoginseng plant for selecting well-grown, no disease and pests harm, acquires Red mature fruit, removes the peel after disinfection to reserve；
2) after by the seed after disinfection using induction liquid immersion 3-5h is sprouted, it is put into the pearl that humidity is maintained at 70-75%
Sprouting is protected from light in batholith matter；
3) after 25-35 days, seed is seeded in floating seedling tray, depth of planting is controlled at 0.5-0.8 centimetres；
4) after 30-45 days, when long to 3-5 centimetres of Radix Notoginseng seedling, floating disc is taken out from floating nutrient solution, being placed in has
On seedling culture frame, moisture control is carried out using spraying machine.
The sterilization method is to sterilize 30min with 0.01% potassium permanganate solution.
The sprouting induces liquid are as follows: is dissolved in the GA of the 0.2mg/L of 1% potassium nitrate aqueous solution3And 5mg/L 6-BA or
The NAA of 2mg/L.
The matrix of the floating seedling tray is made of perlite, fertile soil, vermiculite, peat, and floating nutrient solution is concentration
Contain N, P for 500-800ppm2O5、K2Compound fertilizer's aqueous solution of O；Wherein perlite, fertile soil, vermiculite, peat volume ratio are 1:1:
0.5:2, N:P2O5: K2The mass ratio of O is 16:10:14.
The condition that the spraying machine carries out moisture control is that air humidity is controlled in 85-90%, the humidity of culture substrate
Control is in 75-80%.
Compared with the existing technology, the invention has the benefit that
The present invention is sprouted induction, the sport technique segments such as initiation, floating seedlings, seedling stage are managed by spraying with fresh Panax notoginseng seeds,
Can be under the control condition of batch production, the high-quality Radix Notoginseng seedling of high effect culture.
The method of the present invention can not only save seedling fostering soil, can effectively avoid soil-borne disease during Radix Notoginseng seedling fostering
Infect, reduce cultivation of pseudo-ginseng during disease occur, alleviate cultivation of pseudo-ginseng during continuous cropping obstacle.
Specific embodiment
The present invention will be further explained with reference to the examples below, as described below, is only to preferable implementation of the invention
Example, not limits the present invention, any person skilled in the art is possibly also with the disclosure above
Technology contents be changed to the equivalent embodiment changed on an equal basis.Without departing from the concept of the present invention, according to the present invention
Technical spirit any simple modification or equivalent variations that following embodiment is made, fall within the scope of protection of the present invention.
The invention mainly relates to the contents of four aspects:
Firstly, carrying out vernalization using plant hormone using fresh seeds, avoid the pathogen during seed after ripening dirty
Dye.
Secondly, carrying out seed priming during presprouting of seeds using perlite and polyethylene glycol, avoiding seed and sprouting
In the process, the germination rate brought due to moisture, temperature change is low.
Third carries out seedling management using floating seedlings, reduces influence of the seedling stage environment to Radix Notoginseng seedling.
4th, using spraying water management, the moisture in seedling stage is effectively controlled, reduces the disease incidence of Radix Notoginseng seedling.
5th, when transplanting, seedling is the cultivation of soil-carried disk, avoids the damage to Radix Notoginseng seedlings root, can reduce native biography
The generation of disease.
Embodiment 1
Select well-grown, no disease and pests harm 3 years raw sangqi ginseng plant as breeding maternal plant, annual November, when fruit is red ripe
When, Radix Notoginseng fruit is acquired, pericarp is removed.Clear water flushes three times, and is then sterilized 30 minutes with 0.01% potassium permanganate solution,
The Panax notoginseng seeds of sanitized are put into sprouting induction treatment liquid and are handled 3-5 hours.Sprout induction treatment fluid are as follows: be dissolved in
The GA of the 0.2mg/L of 1% potassium nitrate aqueous solution3And the NAA of the 6-BA or 2mg/L of 5mg/L.
After being disposed, it will sprout and induce processed seed to be put into humidity and be maintained in the perlite of 70-75% to be protected from light
Processing 25-35 days, at this point, seed can gradually be sprouted.
Seed is seeded in floating seedling tray, depth of planting is controlled at 0.5-0.8 centimetres.Floating disc is the polyphenyl in 162 holes
Vinyl plastics foam floats disk.The matrix of floating seedlings is perlite: fertile soil: vermiculite: peat=1:1:0.5:2 (volume ratio),
Floating nutrient solution is N:P2O5: K2O=16:10:14, concentration are compound fertilizer's aqueous solution of 500-800ppm.
After floating seedlings 30-45 days, when long to 3-5 centimetres of Radix Notoginseng seedling, floating disc is taken out from floating nutrient solution, is placed in
With on seedling culture frame, moisture control is carried out using spraying machine.Air humidity is controlled in 85-90%, and cultivation matrix humid control exists
75-80% carries out one time of nutrition supplement, MS base after the modification that nutritional supplementation liquid is 1/4 to cultivation matrix in every 15-20 days therebetween
Basal culture medium, wherein the potassium nitrate substitution of ammonium nitrate equimolar concentration, the calcium nitrate of two times of molar concentrations of potassium chloride substitute.
Claims (4)
1. a kind of Radix Notoginseng factorial seedling-culturing method, it is characterised in that include the following steps:
(1) the Radix Notoginseng plant for selecting well-grown, no disease and pests harm, acquires Red mature fruit, removes the peel after disinfection to reserve；
(2) after by the seed after disinfection using induction liquid immersion 3-5h is sprouted, it is put into the pearl batholith that humidity is maintained at 70-75%
Sprouting is protected from light in matter；
(3) after 25-35 days, seed is seeded in floating seedling tray, depth of planting is controlled at 0.5-0.8 centimetres；
(4) after 30-45 days, when long to 3-5 centimetres of Radix Notoginseng seedling, floating disc is taken out from floating nutrient solution, is placed in nursery
On frame, moisture control is carried out using spraying machine；The matrix of the floating seedling tray is by perlite, fertile soil, vermiculite, peat group
At floating nutrient solution is that concentration is 500-800ppm containing N, P2O5、K2Compound fertilizer's aqueous solution of O；The perlite, fertile soil,
Vermiculite, peat volume ratio are 1:1:0.5:2, the N:P2O5: K2The mass ratio of O is 16:10:14.
2. a kind of Radix Notoginseng factorial seedling-culturing method according to claim 1, which is characterized in that the sterilization method is to use
0.01% potassium permanganate solution sterilizes 30min.
3. a kind of Radix Notoginseng factorial seedling-culturing method according to claim 1, which is characterized in that the sprouting induces liquid
Are as follows: it is dissolved in the GA of the 0.2mg/L of 1% potassium nitrate aqueous solution3And the NAA of the 6-BA or 2mg/L of 5mg/L.
4. a kind of Radix Notoginseng factorial seedling-culturing method according to claim 1, which is characterized in that
The condition that the spraying machine carries out moisture control is that air humidity is controlled in 85-90%, and culture substrate humid control exists
75-80%.
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| CN201510759431.0A CN105340708B (en) | 2015-11-10 | 2015-11-10 | A kind of Radix Notoginseng factorial seedling-culturing method |
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| CN201510759431.0A CN105340708B (en) | 2015-11-10 | 2015-11-10 | A kind of Radix Notoginseng factorial seedling-culturing method |
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| Publication Number | Publication Date |
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| CN105340708A CN105340708A (en) | 2016-02-24 |
| CN105340708B true CN105340708B (en) | 2018-12-14 |
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| CN201510759431.0A Expired - Fee Related CN105340708B (en) | 2015-11-10 | 2015-11-10 | A kind of Radix Notoginseng factorial seedling-culturing method |
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Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105993446A (en) * | 2016-05-16 | 2016-10-12 | 玉溪市祥馨农业技术开发有限公司 | High-yield planting method of panax notoginseng |
| CN105993871B (en) * | 2016-05-16 | 2019-10-22 | 杜云龙 | A kind of fast seedling-cultivating method promoting the high germination rate of Radix Notoginseng using branch acremonium |
| CN106305396A (en) * | 2016-08-24 | 2017-01-11 | 文山苗乡三七科技有限公司 | Soilless culture substrate of radix notoginseng |
| CN108651249B (en) * | 2018-03-23 | 2020-05-19 | 湖北省农业科学院中药材研究所 | Three-dimensional seedling culture method for panax japonicus |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101164409A (en) * | 2007-09-11 | 2008-04-23 | 楚雄医药高等专科学校 | Floatation seedling method for traditional Chinese medicine |
| CN101822201A (en) * | 2010-05-07 | 2010-09-08 | 云南远益园林工程有限公司 | Rhododendron delavayi float seedling method |
| CN102627494A (en) * | 2012-03-25 | 2012-08-08 | 云南远益园林工程有限公司 | Floating seedling and cultivation medium for pseudo-ginseng seed |
| CN102627495A (en) * | 2012-03-25 | 2012-08-08 | 云南远益园林工程有限公司 | Floating seedling nutrient solution for Chinese herbal medicine plant |
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2015
- 2015-11-10 CN CN201510759431.0A patent/CN105340708B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101164409A (en) * | 2007-09-11 | 2008-04-23 | 楚雄医药高等专科学校 | Floatation seedling method for traditional Chinese medicine |
| CN101822201A (en) * | 2010-05-07 | 2010-09-08 | 云南远益园林工程有限公司 | Rhododendron delavayi float seedling method |
| CN102627494A (en) * | 2012-03-25 | 2012-08-08 | 云南远益园林工程有限公司 | Floating seedling and cultivation medium for pseudo-ginseng seed |
| CN102627495A (en) * | 2012-03-25 | 2012-08-08 | 云南远益园林工程有限公司 | Floating seedling nutrient solution for Chinese herbal medicine plant |
Non-Patent Citations (1)
| Title |
|---|
| 三七工厂化育苗可持续发展能力评价指标体系初探;杨文彩等;《广东农业科学》;20121231(第22期);第31-32页 * |
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