Fructus actinidiae chinensis artificial pollination method and Kiwi fruit pollen used prepare method
Technical field
The invention belongs to agriculture field, particularly to a kind of Kiwi fruit pollen production technology and artificial pollination maneuver.
Background technology
Kiwi berry nutritious abundant, rich in vitamin C and various trace element, its sarcocarp is delicate, give off a strong fragrance, sweet mouthfeel is salubrious, acidity is extremely low, has " title of magical delicious fruit ". In recent years, the development speed of China's Fructus actinidiae chinensis is very fast, and whole nation cultivated area has reached more than 500,000 mus at present. Current existing Fructus actinidiae chinensis is male and female plant mixed culture pattern, i.e. be the land for growing field crops pergola system of routine.
Owing to Fructus actinidiae chinensis is dioecian plant, wanting the fruit that obtains higher yield and high-quality on producing, artificial pollination is requisite measure, and especially the time at florescence of Fructus actinidiae chinensis is short, relies on open-pollinated it is difficult to ensure that good quality and high output. But in actual production, Actinidia strain is opened the time spent and is suffered from the impact of Different climate condition, and does not meet, WUHUAFEN or have pollen and female plant is not bloomed when causing artificial pollination. Further, since the persistent period that Fructus actinidiae chinensis is bloomed is about 7 days, and the best pollination time of female flower only has the time limit of 2~3 days, and artificial pollination workload is big, blooms be less susceptible to pollination if running into the Pluvial, and orchard this defect slightly larger at area seems more prominent. Therefore, necessary existing Kiwi fruit pollen production technology and artificial pollination operational approach are carried out innovates improvement, and guarantee Fructus actinidiae chinensis produces higher fruit-setting rate and fruit quality.
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of Fructus actinidiae chinensis artificial pollination method and corresponding Kiwi fruit pollen prepares method, and Fructus actinidiae chinensis production has higher fruit-setting rate and fruit quality to adopt the method for the present invention can guarantee that.
In order to solve above-mentioned technical problem, the present invention provides a kind of Kiwi fruit pollen to prepare method, comprises the following steps:
A, male and female plant are preferably 20:1 according to 19~21:1() quantity ratio configure, staminiferous plant be facility plastic greenhouse cultivation, female plant is land for growing field crops pergola system;
B, enter after florescence staminiferous plant, carry out the collection of pollen, be sequentially carried out following steps:
Pluck male flower: in the morning before 9, select the male flower (that is, presenting the male flower of " small bell " shape) that will open, pluck from anthocaulus;
Take flower pesticide and the flower pesticide that dries in the air: after taking out the tiling of the flower pesticide in male flower, carry out airing, until flower pesticide will not cohere mutually, terminate airing;
Sieve pollen and storage: the flower pesticide after airing is crushed to the sieve that can cross at least 150 orders; It is then placed in container and stores under the room temperature of 10~25 DEG C or store under the refrigerated storage temperature of 0~5 DEG C; Obtain Kiwi fruit pollen;
Storage period under described room temperature is 1 week, and the storage period under described refrigerated storage temperature is 30~40 days.
The improvement of method is prepared as the Kiwi fruit pollen of the present invention:
Step A is for being sequentially carried out following steps:
A), female plant and staminiferous plant configure according to the quantity ratio of 19~21:1;
B), staminiferous plant be facility plastic greenhouse cultivation, seeding row spacing is 3 meters × 2 meters; Female plant is land for growing field crops pergola system, seeding row spacing 4 meters × 3 meters;
The cultivation of described facility plastic greenhouse is the planting type of plastic film shed, when Fructus actinidiae chinensis enters leaf stretching period, staminiferous plant carries out button canopy and processes.
Remarks illustrate: all the other planting patternss are with the plantation of conventional Fructus actinidiae chinensis.
The present invention also provides the Fructus actinidiae chinensis artificial pollination method that a kind of Kiwi fruit pollen utilizing said method to prepare and to obtain carries out simultaneously, and after female flower enters florescence, utilization is positioned at the Kiwi fruit pollen of storage period and carries out artificial pollination; Including:
As come into bloom (during the female plant amount < 30% that Ji Quan is bloomed in garden), namely when female plant does not have large area to bloom, pollination pen point is adopted to award method (for method I): when female flower is open, at 7~10 in morning on non-rainy day or pollinate afternoon 4~7;
When full-bloom stage (during female plant amount >=30% that Ji Quan is bloomed in garden), when namely female plant large area is bloomed, adopt atomizing pollination device dry powder spraying method (for method II): when female flower is open, at 7~10 in morning on non-rainy day or pollinate afternoon 4~7.
Remarks illustrate: above-mentioned 2 kinds of methods with the use of.
Improvement as the Fructus actinidiae chinensis artificial pollination method of the present invention:
The described pollination pen point method of awarding is: after making the light libation at an ancient wedding ceremony pollen of pollination pen, touch pistil stigma, thus realizing pollination.
Described atomizing pollination device dry powder spraying method is:
Kiwi fruit pollen and water milling powder or glutinous rice flour after Homogeneous phase mixing, are obtained pollen mixture according to the quality ratio of 1:1; Pollen mixture is placed in atomizing pollination device, makes the spout of atomizing pollination device be positioned at 5~8 centimetres below female flower; Thus realizing being sprayed onto Kiwi fruit pollen female taking.
Technical problem solved by the invention and corresponding technical advantage be:
One), change traditional Fructus actinidiae chinensis and produce the cultivation mode according to the configuration of male and female plant certain proportion, but produce the pollen quantity needed for area according to Fructus actinidiae chinensis, the facility plastic greenhouse cultivation of staminiferous plant is carried out in defined area, production base, concentrate the production carrying out pollen, the whole field planting female plant in other region (being conventional land for growing field crops pergola system) in base. Adopt this cultivation mode can concentrate the production carrying out Kiwi fruit pollen, and staminiferous plant Blooming can be made by facility plastic greenhouse cultivation, pollen after collection can be undertaken preserving (cold preservation of 0~5 DEG C is best mode) by the cold preservation of 0~5 DEG C or the mode of room temperature, artificial pollination is carried out when female plant enters florescence, avoid staminiferous plant when Fructus actinidiae chinensis female plant is bloomed not bloom, it does not have the predicament that pollen is pollinated with it.
Remarks illustrate: under existing male and female plant mixed culture pattern, male and female plant allocation ratio is 8~10:1.
Two), artificial pollination method can adopt the mode that pollination pen point awards method and/or atomizing pollination device dry powder spraying method combines rationally to carry out artificial pollination.
To award method Pollination Effect good for pollination pen point, but relatively time-consuming takes a lot of work; And pollination device dry powder spraying method time and labour saving, but need pollen amount more. In actual production, female plant comes into bloom and blooms number seldom, is now suitable for adopting pollination pen point to award method; If but weather is fine continuously, female plant quickly enters full-bloom stage, and substantial amounts of female flower needs artificial pollination, is now suitable for adopting pollination device dry powder spraying method.
Adopt aforesaid way, can ensure that Fructus actinidiae chinensis production has higher fruit-setting rate and fruit quality.
Accompanying drawing explanation
Below in conjunction with accompanying drawing, the specific embodiment of the present invention is described in further detail.
Fig. 1 is a kind of atomizing pollination device obtained by commercial mode that the present invention selects.
Detailed description of the invention
Below in conjunction with example, the present invention will be further described.
Embodiment 1, utilizing the Fructus actinidiae chinensis artificial pollination method that Kiwi fruit pollen carries out, selected Kiwifruit Cultivars is red kiwi fruit, is divided into following 2 major parts:
One, Kiwi fruit pollen prepares method, is sequentially carried out following steps (to plant) in Zhejiang Province:
A, male and female plant plantation:
A), annual December is to, at the beginning of next year 3, carrying out field planting female plant by seeding row spacing 4 meters × 3 meters, i.e. female plant is land for growing field crops pergola system; Field planting staminiferous plant is carried out according to seeding row spacing 3 meters × 2 meters, i.e. staminiferous plant is facility plastic greenhouse cultivation in the region that can build hot house;
The quantity of the staminiferous plant of field planting configures in the ratio of male and female plant 20:1.
B), owing to being annual mid or late April (under the state of land for growing field crops) florescence of area, Zhejiang Province red kiwi fruit, therefore staminiferous plant starts to detain canopy (be generally in Zhejiang Province annual March 15 before and after for leaf stretching period) when Fructus actinidiae chinensis enters leaf stretching period, now needs staminiferous plant carries out button canopy (i.e. covered with plastic film);
Remarks illustrate: staminiferous plant is additionally required outside button canopy, and the measure of other cultivation is the same with female plant, for conventional kiwi fruit plant planting type.
After B, staminiferous plant button canopy, generally through the time of about 30 days, initially enter florescence; It is generally in Zhejiang Province before and after annual April 15. Now, being performed continuously over the collection of pollen according to following steps, the time that can gather pollen is about 7 days:
Pluck male flower: carry out in the past in the morning at 9, select the male flower (namely presenting the male flower of " small bell " shape) that will open, pluck from anthocaulus and bring back.
Take flower pesticide: first take two clean newspapers, divide on underground or table, the male flower that will adopt back, break petal into two with one's hands, pluck with hands or with toothbrush, its flower pesticide brushed on newspaper.
Dry in the air flower pesticide: by the flower pesticide under brush, is put into dry, ventilation airing. Such as sombre weather, can be put on electric blanket or heat under lamp of heating, drying (noting, heating-up temperature not can exceed that 40 DEG C); Until pollen does not cohere mutually, terminate airing.
Sieve pollen and storage: just the flower pesticide after airing is crushed to the sieve that can cross at least 150 orders; It is then charged into clean bottle is preserved standby. Can store 1 week under room temperature (10~25 DEG C), or Pollen Activity can be kept 30~40 days by 0~5 DEG C of cold preservation.
To be storage period 0 day, store 1 week at 25 DEG C, the Kiwi fruit pollen stored 30~40 days under 0~5 DEG C of cold preservation carries out the detection of following vigor performance:
Pollen Activity measures and adopts tetrazolium salts method: the index studied as Pollen Activity using pollen staining rate.Adding pollen grain with the chlorinated triphenyl base four chlorazol (TTC) of 4% and make section, be placed in the baking oven of 37 DEG C, be positioned over optical microphotograph Microscopic observation after 30min, what display was red is great-hearted pollen. Repeat for 3 times, 3 visuals field of each repeated observation, calculate its dyeing percentage rate.
It is 83.6% that result is respectively as follows: pollen (just having plucked) the pollen staining rate that storage period is 0 day; At 25 DEG C, the pollen of storage pollen staining rate in 1~7 day is more than 78.1%, and the pollen staining rate of more than 8 days is below 20%; Within 40 days, pollen staining rate is more than 72.1% for 0~5 DEG C of cold preservation pollen, and after 40 days, pollen staining rate is below 25%.
Two, artificial pollination
In Zhejiang Province, the red kiwi fruit of land for growing field crops pergola system is typically in entering florescence after before annual April 20, the Kiwi fruit pollen that above-mentioned steps one preserves now is utilized to carry out artificial pollination according to following two method, wherein in female flower pollination in open the 2nd to the 3rd day for best.
Remarks illustrate: petal when female flower is open is just budding state, apparent, it is possible to knowing second day and certainly open, a flower can open 2~6 days, and full-bloom stage mostly is 3~4 days. Time just open, the bract of flower does not open completely, and the complete state that the flowers are in blossom is to open completely, and pattern is bright-coloured, and after the flowers are in blossom couple of days, being not as that pattern will become is bright-coloured, and tension force is also without tall and straight in the past. After pollination, the petal of white can quickly become faint yellow.
As come into bloom (during the female plant amount < 30% that Ji Quan is bloomed in garden), when namely female plant does not have large area to bloom, pollination pen point is adopted to award method (method I):
When full-bloom stage (during female plant amount >=30% that Ji Quan is bloomed in garden), when namely female plant large area is bloomed, adopt atomizing pollination device dry powder spraying method (method II).
Specific as follows:
Artificial pollination method I: pollination pen point awards method, is suitable for coming into bloom, and adopts when female plant does not have large area to bloom. Including herein below:
Make pollination pen: adopt common writing brush, cut off apicule part, make pen hair neatly, or the sponge in employing cigarette filter, fine bamboo rod that bind is 20 centimetres, waddy.
Pollinating in field: is poured out on a small quantity by ready pollen (Kiwi fruit pollen of above-mentioned steps one gained), load in clean teacup. When female flower is open, can pollinate after dew (rain) water is dry (morning 7~10 point, 4~7 pollinations in afternoon be advisable, rainy day can not pollinate).
Specifically pollinate operating technology: one hand-held pollen cup during pollination, a hand-held pollination pen, after light libation at an ancient wedding ceremony pollen, pistil stigma docking point gently is awarded. The general libation at an ancient wedding ceremony takes a pollen and can put and award 5~7 female flowers.
Remarks illustrate: adopt above-mentioned artificial pollination method I, 7~8 grams of pure pollen of 1 mu of need.
Artificial pollination method II: Kiwi pollinator dry powder spraying method, Kiwi pollinator selects atomizing pollination device succinct on market, as shown in Figure 1. This atomizing pollination device is held bottle 1 by pollen and air-blowing capsule 2 forms. This method is suitable at full-bloom stage, adopts when female plant large area is bloomed. Including herein below:
The making of pollen dry powder: Kiwi fruit pollen above-mentioned steps one preserved is mixed homogeneously according to 1:1 ratio with water milling powder or glutinous rice flour, the pollen pouring Kiwi pollinator into holds in bottle 1.
Specifically pollinate operating technology in field: when female flower is open, can pollinate after dew (rain) water is dry (morning 7~10 point, 4~7 pollinations in afternoon be advisable, rainy day can not pollinate).During pollination, the Kiwi pollinator of hand-held loading pollen, below female flower, pollen is sprayed onto female taking by 5~8 centimeters equably. Spray once can pollinate to 15~20 female flowers simultaneously simultaneously.
Remarks illustrate: with above-mentioned artificial pollination method II, 14~16 grams of pure pollen of 1 mu of need.
All the other cultivation conditions of red kiwi fruit compared with the cultivation condition of conventional red kiwi fruit.
Adopting aforesaid way, Fructus actinidiae chinensis produces higher fruit-setting rate and fruit quality. Particularly as follows: pollination one week after measures fruit-setting rate up to more than 90%, after gathering measure fruit quality as: fruit single fruit weight on average (does not use lower 93 grams of any growth hormone situation up to 93 grams, up to 112 grams when use growth hormone), soluble solid content is up to 20.8%.
Embodiment 2, utilizing the Fructus actinidiae chinensis artificial pollination method that Kiwi fruit pollen carries out, selected Kiwifruit Cultivars is Xu Xiang Fructus actinidiae chinensis, is divided into following 2 major parts:
One, Kiwi fruit pollen prepares method, is sequentially carried out following steps (to plant) in Zhejiang Province:
A, male and female plant plantation:
A), selected Kiwifruit Cultivars be Xu Xiang Fructus actinidiae chinensis, annual December is to, at the beginning of next year 3, carrying out field planting female plant by seeding row spacing 4 meters × 3 meters, i.e. female plant is land for growing field crops pergola system; Field planting staminiferous plant is carried out according to seeding row spacing 3 meters × 2 meters, i.e. staminiferous plant is facility plastic greenhouse cultivation in the region of frame of can puing up a shed; The quantity of field planting staminiferous plant configures in the ratio of male and female plant 20:1.
B), the florescence of area, Zhejiang Province Xu Xiang Fructus actinidiae chinensis is annual by the end of April at the beginning of 5 months (under the state of land for growing field crops), staminiferous plant is typically in starting to detain canopy when Fructus actinidiae chinensis enters exhibition leaf, being generally in Zhejiang Province before and after annual March 25, other cultivation step need button canopy except staminiferous plant except is the same with female plant;
After B, staminiferous plant button canopy, generally through the time of about 30 days, initially enter florescence, be generally in Zhejiang Province before and after annual April 25.. Now, being performed continuously over the collection of pollen according to following steps, the time that can gather pollen is about 7 days:
Pluck male flower: carry out in the past in the morning at 9, select the male flower (namely presenting the male flower of " small bell " shape) that will open, pluck from anthocaulus and bring back.
Take flower pesticide: first take two clean newspapers, divide on underground or table, the male flower that will adopt back, break petal into two with one's hands, pluck with hands or with toothbrush, its flower pesticide brushed on newspaper.
Dry in the air flower pesticide: by the flower pesticide under brush, is put into dry, ventilation airing. Such as sombre weather, can be put on electric blanket or heat under lamp of heating, drying (noting, heating-up temperature not can exceed that 40 DEG C); Until pollen does not cohere mutually, terminate airing.
Sieve pollen and storage: just the flower pesticide after airing is crushed to the sieve that can cross at least 150 orders; It is then charged into clean bottle is preserved standby. Can store 1 week under room temperature (10~25 DEG C), or Pollen Activity can be kept 30~40 days by 0~5 DEG C of cold preservation.
Two, artificial pollination
In Zhejiang Province, Xu Xiang Fructus actinidiae chinensis is typically in entering florescence after before annual May 1, now utilizes the pollen of gained in above-mentioned steps to carry out artificial pollination according to following two method, wherein hangs most in female flower pollination in open the 2nd to the 3rd day.
Artificial pollination method I: pollination pen point awards method, is suitable at come into bloom (during the female plant amount < 30% that Ji Quan is bloomed in garden), i.e. adopt when female plant does not have large area to bloom.
Make pollination pen: adopt common writing brush, cut off apicule part, make pen hair neatly; Or the sponge in employing cigarette filter, fine bamboo rod that bind is 20 centimetres, waddy.
Pollinating in field: is poured out on a small quantity by ready pollen, load in clean teacup. When female flower is open, can pollinate after dew (rain) water is dry (morning 7~10 point, 4~7 pollinations in afternoon be advisable, rainy day can not pollinate).
Specifically pollinate operating technology: one hand-held pollen cup during pollination, a hand-held pollination pen, after light libation at an ancient wedding ceremony pollen, pistil stigma docking point gently is awarded. The general libation at an ancient wedding ceremony takes a pollen and can put and award 5~7 female flowers.
Remarks illustrate: adopt above-mentioned artificial pollination method I, 7~8 grams of pure pollen of 1 mu of need.
Artificial pollination method II: Kiwi pollinator dry powder spraying method, Kiwi pollinator selects atomizing pollination device succinct on market, as shown in Figure 1. This method is suitable at full-bloom stage (during female plant amount >=30% that Ji Quan is bloomed in garden), i.e. adopt when female plant large area is bloomed.
The making of pollen dry powder: the pollen of above-mentioned steps gained is mixed homogeneously according to 1:1 ratio with water milling powder or glutinous rice flour, pours Kiwi pollinator into.
Specifically pollinate operating technology in field: when female flower is open, can pollinate after dew (rain) water is dry (morning 7~10 point, 4~7 pollinations in afternoon be advisable, rainy day can not pollinate). During pollination, the Kiwi pollinator of hand-held loading pollen, below female flower, pollen is sprayed onto female taking by 5~8 centimeters equably. Spray once can pollinate to 15~20 female flowers simultaneously simultaneously.
Remarks illustrate: with above-mentioned artificial pollination method II, 14~16 grams of pure pollen of 1 mu of need.
All the other cultivation conditions of Xu Xiang Fructus actinidiae chinensis compared with the cultivation condition of conventional Xu Xiang Fructus actinidiae chinensis.
Adopting aforesaid way, Fructus actinidiae chinensis produces higher fruit-setting rate and fruit quality. Be specially pollination one week after measure fruit-setting rate up to more than 90%, after gathering measure fruit quality as: fruit single fruit weight is on average up to 108 grams, and soluble solid content is up to 17%.
Comparative example 1, the red kiwi fruit described in embodiment 1 is planted according to prior art, i.e. annual December is to, at the beginning of next year 3, carrying out field planting female plant and staminiferous plant by seeding row spacing 4 meters × 3 meters; The ratio of male and female plant is 10:1; Planting in a conventional manner, artificial pollination mode is put honeybee in spontaneous pollination or orchard.
As a result, the fruit-setting rate of Fructus actinidiae chinensis is only 76%, and it is general less that fruit quality shows as fruit single fruit weight, average 73.6 grams, and size is uneven all, and soluble solid content is typically in about 19%.
Comparative example 2, the Xu Xiang Fructus actinidiae chinensis described in embodiment 2 is planted according to prior art, i.e. annual December is to, at the beginning of next year 3, carrying out field planting female plant and staminiferous plant by seeding row spacing 4 meters × 3 meters; The ratio of male and female plant is 10:1; Planting in a conventional manner, artificial pollination mode is put honeybee in spontaneous pollination or orchard.
As a result, the fruit-setting rate of Fructus actinidiae chinensis is only 78%, and it is general less that fruit quality shows as fruit single fruit weight, average 80.6 grams, and size is uneven all, and soluble solid content is typically in about 15.8%.
Finally, in addition it is also necessary to be only several specific embodiments of the present invention it is noted that listed above. It is clear that the invention is not restricted to above example, it is also possible to there are many deformation. All deformation that those of ordinary skill in the art can directly derive from present disclosure or associate, are all considered as protection scope of the present invention.