CN104472342A - Pollinating solution for kiwi fruit atomization pollination method, as well as preparation method thereof - Google Patents
Pollinating solution for kiwi fruit atomization pollination method, as well as preparation method thereof Download PDFInfo
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- CN104472342A CN104472342A CN201410698539.9A CN201410698539A CN104472342A CN 104472342 A CN104472342 A CN 104472342A CN 201410698539 A CN201410698539 A CN 201410698539A CN 104472342 A CN104472342 A CN 104472342A
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- 235000009436 Actinidia deliciosa Nutrition 0.000 title claims abstract description 60
- 238000000034 method Methods 0.000 title claims abstract description 28
- 238000002360 preparation method Methods 0.000 title claims abstract description 11
- 238000000889 atomisation Methods 0.000 title abstract 2
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- 229930006000 Sucrose Natural products 0.000 claims abstract description 15
- KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical compound OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 claims abstract description 15
- 239000004327 boric acid Substances 0.000 claims abstract description 15
- 239000008103 glucose Substances 0.000 claims abstract description 15
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- 238000007789 sealing Methods 0.000 claims abstract description 9
- 239000000126 substance Substances 0.000 claims abstract description 9
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 claims abstract description 8
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- YGGXZTQSGNFKPJ-UHFFFAOYSA-N methyl 2-naphthalen-1-ylacetate Chemical compound C1=CC=C2C(CC(=O)OC)=CC=CC2=C1 YGGXZTQSGNFKPJ-UHFFFAOYSA-N 0.000 claims description 18
- 238000005507 spraying Methods 0.000 claims description 18
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- 150000002596 lactones Chemical class 0.000 claims description 10
- UFWIBTONFRDIAS-UHFFFAOYSA-N naphthalene-acid Natural products C1=CC=CC2=CC=CC=C21 UFWIBTONFRDIAS-UHFFFAOYSA-N 0.000 claims description 4
- 238000003860 storage Methods 0.000 claims description 4
- 238000013329 compounding Methods 0.000 claims description 3
- 238000009826 distribution Methods 0.000 claims description 3
- 239000002994 raw material Substances 0.000 claims description 3
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 claims description 2
- 229910021538 borax Inorganic materials 0.000 claims description 2
- 229910052791 calcium Inorganic materials 0.000 claims description 2
- 239000011575 calcium Substances 0.000 claims description 2
- 239000004328 sodium tetraborate Substances 0.000 claims description 2
- 235000010339 sodium tetraborate Nutrition 0.000 claims description 2
- 230000000694 effects Effects 0.000 abstract description 8
- 230000007198 pollen germination Effects 0.000 abstract description 5
- 238000004519 manufacturing process Methods 0.000 abstract description 3
- PRPINYUDVPFIRX-UHFFFAOYSA-N 1-naphthaleneacetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CC=CC2=C1 PRPINYUDVPFIRX-UHFFFAOYSA-N 0.000 abstract 4
- IXVMHGVQKLDRKH-VRESXRICSA-N Brassinolide Natural products O=C1OC[C@@H]2[C@@H]3[C@@](C)([C@H]([C@@H]([C@@H](O)[C@H](O)[C@H](C(C)C)C)C)CC3)CC[C@@H]2[C@]2(C)[C@@H]1C[C@H](O)[C@H](O)C2 IXVMHGVQKLDRKH-VRESXRICSA-N 0.000 abstract 3
- IXVMHGVQKLDRKH-KNBKMWSGSA-N brassinolide Chemical compound C1OC(=O)[C@H]2C[C@H](O)[C@H](O)C[C@]2(C)[C@H]2CC[C@]3(C)[C@@H]([C@H](C)[C@@H](O)[C@H](O)[C@@H](C)C(C)C)CC[C@H]3[C@@H]21 IXVMHGVQKLDRKH-KNBKMWSGSA-N 0.000 abstract 3
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- CCBICDLNWJRFPO-UHFFFAOYSA-N 2,6-dichloroindophenol Chemical compound C1=CC(O)=CC=C1N=C1C=C(Cl)C(=O)C(Cl)=C1 CCBICDLNWJRFPO-UHFFFAOYSA-N 0.000 description 1
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- SEOVTRFCIGRIMH-UHFFFAOYSA-N indole-3-acetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CNC2=C1 SEOVTRFCIGRIMH-UHFFFAOYSA-N 0.000 description 1
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Landscapes
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention discloses a pollinating solution for a kiwi fruit atomization pollination method, as well as a preparation method thereof. The pollinating solution comprises the following components in percentage by weight: 0.04-0.08% of naphthylacetic acid, 0.05-0.5% of saccharose, 0.05-0.5% of boric acid, 83.36-96.89% of water, 0.05-0.50% of urea, 3-15% of glucose, and 0.02-0.06% of brassinolide. The preparation method comprises the following steps: weighing naphthylacetic acid, saccharose, boric acid, urea, glucose and brassinolide; fully mixing saccharose, boric acid, urea, glucose and brassinolide; weighing warm water to dissolve naphthylacetic acid; after the temperature of the naphthylacetic acid solution is reduced, adding other mixed substances and fully stirring and dissolving, and then sealing and storing. The pollinating solution for kiwi fruit is good in stability, has the good effect of promoting the pollen germination of kiwi fruits as well as obvious functions of reducing the labor workload and lowering the production cost.
Description
Technical field
The present invention relates to technique for culturing yangtao field, especially kiwi fruit spraying pollination technique field, specifically a kind of pollination liquid for kiwi fruit spraying pollination method and preparation method thereof.
Background technology
Kiwi fruit, also claim fox peach, bower actinidia root or leaf, Chinese gooseberry, carambola, wood, Mao Muguo, Chinese grooseberry, numb rattan fruit etc., be distributed widely between the southern china mountain ridge.Kiwi fruit is dioecious large-scale fallen leaves bejuco.Staminiferous plant crinosity leaf is little, and staminiferous plant also comparatively early comes across female flower; Female plant lacks hair or without hair, floral leaf is all greater than staminiferous plant.Florescence is 5-June, and fructescence is 8-October.
Under field conditions (factors), mainly by insect pollination, in and orchard that management is meticulous more sufficient at human resources, artificial supplementary pollination is also one of important agricultural measures to kiwi fruit.A large amount of artificial pollination practices shows, better, the more, fruit is individual larger, and quality is also good for the seed of fruit in pollination.Along with the expansion of kiwi fruit industry, conventional pollination technique is more and more awkward, and the most of grower in China various places is now is mostly adopt conventional pollinating method (artificial point awards method) and honeybee supplementary pollination, does not reach the effect that output is high at all.And, conventional artificial pollination method is owing to being subject to the restriction of many reasons, the method is had expend time in long, expend material resources large, expend the shortcomings such as pollen is many, the development that this results in kiwi fruit industry is restricted greatly, and many investors are inspired awe even from distance.
The spraying pollination method utilizing research and development pollination liquid to carry out kiwi fruit can solve this problem, its using method shakes up by pollen directly being added in pollination liquid, utilize sprayer directly to spray to female flower, having like this dusts concentrates accurately, female flower pollination rate is high, pollination speed fast, it is short to expend time in, save the plurality of advantages such as human and material resources and pollen.But, in prior art, desirable achievement is not still obtained for the research of kiwi fruit pollination liquid.
Summary of the invention
The object of the invention is to overcome the deficiencies in the prior art, a kind of pollination liquid and preparation method thereof for kiwi fruit spraying pollination method is provided, this pollination liquid can realize dusting concentrate accurately, female flower fruit-setting rate is high, pollination speed fast, deformed fruit fruit rate is low, the effect of protective yield of guaranteeing the quality is good, save the advantage such as human and material resources and pollen.
The present invention is by the following technical programs to realize this object:
First, the present invention proposes a kind of pollination liquid for kiwi fruit spraying pollination method, carry out spraying by sprayer and pollinate after this pollination liquid is used for mixing with kiwi fruit male flower pollen, wherein, the component of this pollination liquid comprise percentage by weight be 0.04 ~ 0.08% methyl α-naphthyl acetate, the sucrose of 0.05 ~ 0.5%, the boric acid of 0.05 ~ 0.5% and 83.36 ~ 96.89% water.Further, urea, the glucose of 3 ~ 15%, the brassin lactones of 0.02 ~ 0.06% that percentage by weight is 0.05 ~ 0.50% is also comprised in the component of this pollination liquid.Preferably, this pollination liquid is made up of the component of following percentage by weight: methyl α-naphthyl acetate 0.06%, sucrose 0.20%, boric acid 0.20%, urea 0.02%, glucose 8%, brassin lactones 0.04% and water 91.48%.
Wherein, in technical scheme of the present invention, indication methyl α-naphthyl acetate is plant hormone auxin; Indication sucrose refers to decompose the generation D-Glucose of equivalent and the carbohydrate of D-Fructose, comprises the combination of one or more of white granulated sugar, soft white sugar and brown granulated sugar etc.; Indication boric acid is the combination of one or more of boric acid, borax etc.; Indication urea is the neutral quick-acting fertilizers of agriculture articles for use; Indication glucose is one or more the combination such as glucose, calcium gluconae; Indication brassin lactones is a kind of new green environment protection plant growth regulator; Indication water is the combination of one or more of distilled water, deionized water, high purity water and running water etc.
The invention allows for the preparation method of this kiwi fruit pollination liquid, the method comprises the steps:
(1) raw material weighing: per distribution ratio ratio takes methyl α-naphthyl acetate, sucrose, boric acid, urea, glucose, brassin lactones by weight; (2) mix: alleged pollination liquid component substances (except methyl α-naphthyl acetate) is fully mixed; (3) methyl α-naphthyl acetate dissolves: the warm water (20 DEG C ~ 25 DEG C) measuring required ratio first dissolves methyl α-naphthyl acetate; (4) naphthalene acid solution dissolves all the other component substances: after water temperature reduces, add the abundant stirring and dissolving of compounding substances mixed by (2).(5) sealing is preserved: preserve with the sealing such as vial or plastic bottle.
Wherein, sealing preserves that reserve temperature be-10 DEG C ~ 35 DEG C, pollination liquid storage pH is 5 ~ 7.5, storage light dark, normal light and high light, bottling employing vial or plastic bottle, clear vial or brown bottle etc.
Pollination liquid of the present invention is when orchard uses, sprayer spraying pollination is carried out in above-mentioned Kiwi fruit pollen pollination liquid dilution 100 ~ 500 times of liquid (being namely diluted with water to 100 ~ 500 times that former pollination liquid is long-pending), spray amount is that every female plant (300 ~ 450 female flowers) 40 ~ 100ml pollinates liquid (after dilution and add the pollination liquid of pollen), spray timings is 1 ~ 2 time, and sprayer should strictly clean up.When orchard uses, every 1000ml liquid (after dilution) of pollinating adds 0.01 ~ 0.04g pollen and fully shakes up, now with the current.
Pollination liquid of the present invention needs the pollen adding male flower in use, and male flower be captured in front 1 ~ 10 day of pollination, the Selection radio main breed florescence slightly morning, pollen amount many, staminiferous plant.The collection method of pollen: the male flower just opened not containing rainwater, dew taken off be placed on brown paper or straticulation divided by device of writing a prescription and naturally dries in the shade or be placed in that temperature is 20 DEG C ~ 25 DEG C, diel placed by the drying box of humidity 45 ~ 55% for 6 ~ 10 in the morning, until male flower all becomes very crisp from the inside to surface, then rub on 120 ~ 200 object sieves or 1 ~ 3 mm wire netting, reject petal and filigree; Or with small-sized electric cracker, male flower is pulverized, reject petal and filigree after sieve.Pollen storage: collection pollen is loaded in centrifuge tube or bottle and is placed in-25 DEG C ~ 5 DEG C refrigerators and preserves.
The present invention compared with prior art, kiwi fruit pollination liquid of the present invention has good stability, Kiwi fruit pollen is sprouted there is good facilitation, and field is used, and 100 ~ 500 times of liquid kiwi fruit pollination rate (fruit-setting rate) are high, malformed fruit rate is low, it is better to protect product preservation and effect, for carrying out spraying pollination in kiwi fruit efficiency cultivation technology for high, fast, efficient, with low cost, be better than traditional artificial point and award method, there is the remarkable effect reducing work workload and production cost.
Embodiment
Below in conjunction with embodiment, the present invention is described in further detail.
Embodiment 1:
Kiwi fruit pollination formula of liquid is made up of methyl α-naphthyl acetate, sucrose, boric acid, urea, glucose, brassin lactones and water.
(1) kiwi fruit pollination liquid preparation: raw material weighing: per distribution ratio ratio takes methyl α-naphthyl acetate 0.6g, sucrose 2g, boric acid 2g, urea 0.2g, glucose 80g, brassin lactones 0.4g by weight.Mixing: alleged pollination liquid component substances (except methyl α-naphthyl acetate) is fully mixed; Methyl α-naphthyl acetate dissolves: the warm water (20 DEG C ~ 25 DEG C) measuring 934.8ml first dissolves methyl α-naphthyl acetate; Naphthalene acid solution dissolves all the other component substances: after water temperature reduces, and adds the abundant stirring and dissolving of compounding substances of other several material mixing except methyl α-naphthyl acetate; Sealing is preserved: preserve with the sealing such as vial or plastic bottle, completes the kiwi fruit pollination liquid of preparation 1000ml.
(2) kiwi fruit pollination liquid uses: when orchard uses, and by above-mentioned Kiwi fruit pollen pollination liquid dilute with water 200 ~ 500 times of liquid, then shakes up according to the pollen that the pollination liquid after every 1000ml dilution adds 0.02g, now with the current.The kiwi fruit pollination liquid that the following stated is now joined all refers to dilute afterwards and adds the pollination liquid of pollen.Carry out sprayer spraying pollination during use in orchard, spray amount is every female plant (300 ~ 400 female flowers) 50ml pollination liquid (dilutes rear and add pollen), and spray timings is 1 time, and sprayer should strictly clean up.
(3) kiwi fruit pollination liquid stabilizing effect measures:
The impact on pollination liquid stability of A, different reserve temperature measures:
Liquid of being pollinated by the kiwi fruit of now joining is that standard loads corresponding transparent conical flask with 200ml, is placed on the condition second half year that temperature is 15 DEG C, 25 DEG C, 35 DEG C, and routine observation record.
B, the different pH impact on pollination liquid stability measures:
Liquid of being pollinated by the kiwi fruit of now joining is that standard loads corresponding transparent conical flask with 200ml, is placed on the condition next month that PH is 5.5,6.0,6.5,7.0 and 7.5, and routine observation record.
The impact on pollination liquid stability of C, different light measures:
Liquid of being pollinated by the kiwi fruit of now joining is that standard loads corresponding transparent conical flask and Brown Glass Brown glass bottles and jars only with 200ml, is placed on the condition next month of high light, normal condition and dark, and routine observation record.
D, pollination liquid measure the impact of pollen germination:
Pollination drop is spread out on cover glass, pollen is evenly dispersed on pollination liquid, pollen amount on often kind of pollination liquid should keep roughly the same, cover glass upset is put on concavity slide, put several wet cotton balls soaking into clear water around, and be put in the identical insulating box of temperature and humidity and cultivate, after 4 hours, examine under a microscope pollen germination rate.
E, pollination liquid measure the impact that kiwi fruit is beared fruit:
After liquid pollinates to kiwi fruit female flower in kiwi fruit pollination, record has been carried out to a number of kiwi fruit female flower, kiwi fruit thank spend after 30 days investigation kiwi fruits to bear fruit number.
F, pollination liquid measure the impact of kiwi fruit Yield and qualities:
After fruit maturation, by community results, measure each community single plant yield, each community random acquisition 200 fruits, take back experimental determination fruit quality, single fruit weight, transverse diameter and vertical footpath, calculate fruit shape index.Nutrient quality assay method: Soluble adhesion molecule anthrone colorimetry measures; The assay method of dry matter and moisture in the content GB8858-1988 fruit of dry matter, victual; Vitamin C content 2,6-dichloroindophenol method measures; Titratable acid content determination of acid-basetitration.
(4) technique effect of embodiment 1:
As shown in table 1,2,3, wherein table 1 illustrates kiwi fruit pollination liquid study on the stability result, table 2 illustrates kiwi fruit pollination liquid affects result to Kiwi fruit pollen germination rate, fruit-setting rate and malformed fruit rate, and what table 3 illustrated on kiwi fruit, kiwi fruit pollination liquid protects that product guarantees the quality (adopt and measure quality in latter 7 days) affects result.
Carry out reserve temperature, pH, illumination investigation (see table 1) to this kiwi fruit pollination liquid, result shows that pollination liquid stability is better.Can draw from table 2 and table 3, compare with clear water pollen liquid, kiwi fruit pollination liquid makes pollen germination rate reach 66.03%, kiwi fruit fruit-setting rate reaches 91.49%, deformed fruit fruit rate is only 4.32%, improve the Vitamin C content of kiwi fruit 15.70%, 34.15% Soluble adhesion molecule, the dry matter content of 8.11%, the titratable acid content, the sugar-acid ratio that reduce by 1.52% reach 7.24, and mean fruit weight 18.94% can be improved and improve kiwi fruit fruit shape index; Compared with awarding with artificial point, every quality and yield index content is suitable, and difference is not remarkable.This kiwi fruit pollination liquid field uses 200 ~ 500 times, and liquid pollination rate is high, guarantor's product preservation and effect is good, be applied on kiwi fruit and carry out spraying pollination, fast, efficient, with low cost, can overcome Traditional Man pollination expend time in long, expend material resources large, expend the shortcomings such as pollen is many, play the effect reducing work workload and production cost, alternative traditional artificial point awards method.
Certainly, it is more than embody rule example of the present invention, the present invention also has other embodiment, anyly do not depart from technical solution of the present invention content, the any simple modification done above embodiment according to technical spirit of the present invention, equivalent variations and modification, all still belong in the scope of technical solution of the present invention.
Claims (6)
1. the pollination liquid for kiwi fruit spraying pollination method, carry out spraying by sprayer and pollinate after this pollination liquid is used for mixing with kiwi fruit male flower pollen, it is characterized in that: the component of this pollination liquid comprise percentage by weight be 0.04 ~ 0.08% methyl α-naphthyl acetate, the sucrose of 0.05 ~ 0.5%, the boric acid of 0.05 ~ 0.5% and 83.36 ~ 96.89% water.
2. the pollination liquid for kiwi fruit spraying pollination method according to claim 1, is characterized in that: also comprise urea, the glucose of 3 ~ 15%, the brassin lactones of 0.02 ~ 0.06% that percentage by weight is 0.05 ~ 0.50% in the component of this pollination liquid.
3. the pollination liquid for kiwi fruit spraying pollination method according to claim 2, is characterized in that: this pollination liquid is made up of the component of following percentage by weight: methyl α-naphthyl acetate 0.06%, sucrose 0.20%, boric acid 0.20%, urea 0.02%, glucose 8%, brassin lactones 0.04% and water 91.48%.
4. the pollination liquid for kiwi fruit spraying pollination method according to claim 3, is characterized in that: described sucrose can replace with glucose or calcium gluconae, and described boric acid can replace with borax.
5. a preparation method for the pollination liquid for kiwi fruit spraying pollination method as described in above-mentioned any claim, is characterized in that the method comprises the steps:
1) raw material weighing: per distribution ratio ratio takes methyl α-naphthyl acetate, sucrose, boric acid, urea, glucose, brassin lactones by weight;
2) mix: sucrose, boric acid, urea, glucose and brassin lactones are fully mixed;
3) methyl α-naphthyl acetate dissolves: the warm water measuring 20 DEG C ~ 25 DEG C in proportion first dissolves methyl α-naphthyl acetate;
4) naphthalene acid solution dissolves all the other component substances: until naphthalene acid solution water temperature reduce after, add step 2) mixed by the abundant stirring and dissolving of compounding substances;
5) sealing is preserved: preserve with vial or plastic bottle sealing.
6. the preparation method of the pollination liquid for kiwi fruit spraying pollination method according to claim 5, is characterized in that: liquid reserve temperature of pollinating when sealing is preserved in step 4) is-10 DEG C ~ 35 DEG C, pollination liquid storage pH is 5 ~ 7.5.
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| CN201410698539.9A CN104472342B (en) | 2014-11-28 | 2014-11-28 | A kind of pollination liquid of pollination method of spraying for Fructus actinidiae chinensis and preparation method thereof |
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| CN201410698539.9A CN104472342B (en) | 2014-11-28 | 2014-11-28 | A kind of pollination liquid of pollination method of spraying for Fructus actinidiae chinensis and preparation method thereof |
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Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105165597A (en) * | 2015-08-07 | 2015-12-23 | 江苏丘陵地区镇江农业科学研究所 | Pollination method for kiwifruit |
| CN105994266A (en) * | 2015-12-29 | 2016-10-12 | 龙友华 | Actinidia spray pollination liquid and preparation method thereof |
| CN106106128A (en) * | 2016-06-30 | 2016-11-16 | 固镇县华丰农业有限公司 | A kind of artificial pollination method of Fructus actinidiae chinensis |
| CN106212071A (en) * | 2016-06-19 | 2016-12-14 | 崔丽红 | A kind of method that induction Fructus actinidiae chinensis " summer millet " kind parthenocarpy produces stenospermocarpy |
| CN106416880A (en) * | 2016-09-07 | 2017-02-22 | 山东省烟台市农业科学研究院 | Pollen nourishing liquid for spraying pollination of sweet cherries, and preparation method and using method thereof |
| CN107155775A (en) * | 2017-06-04 | 2017-09-15 | 贵州村华秋实现代生态农业有限公司 | A kind of implantation methods of red kiwifruit |
| CN107183031A (en) * | 2017-06-04 | 2017-09-22 | 贵州村华秋实现代生态农业有限公司 | A kind of pollination liquid of red kiwifruit spraying pollination method |
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| CN110384112A (en) * | 2019-08-22 | 2019-10-29 | 四川华胜农业股份有限公司 | A kind of Kiwi berry pollination liquid and preparation method and Kiwi berry pollinating method |
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| CN105165597A (en) * | 2015-08-07 | 2015-12-23 | 江苏丘陵地区镇江农业科学研究所 | Pollination method for kiwifruit |
| CN105994266A (en) * | 2015-12-29 | 2016-10-12 | 龙友华 | Actinidia spray pollination liquid and preparation method thereof |
| CN106212071A (en) * | 2016-06-19 | 2016-12-14 | 崔丽红 | A kind of method that induction Fructus actinidiae chinensis " summer millet " kind parthenocarpy produces stenospermocarpy |
| CN106106128A (en) * | 2016-06-30 | 2016-11-16 | 固镇县华丰农业有限公司 | A kind of artificial pollination method of Fructus actinidiae chinensis |
| CN106416880A (en) * | 2016-09-07 | 2017-02-22 | 山东省烟台市农业科学研究院 | Pollen nourishing liquid for spraying pollination of sweet cherries, and preparation method and using method thereof |
| CN107183031A (en) * | 2017-06-04 | 2017-09-22 | 贵州村华秋实现代生态农业有限公司 | A kind of pollination liquid of red kiwifruit spraying pollination method |
| CN107155775A (en) * | 2017-06-04 | 2017-09-15 | 贵州村华秋实现代生态农业有限公司 | A kind of implantation methods of red kiwifruit |
| CN107926977A (en) * | 2017-12-06 | 2018-04-20 | 丹阳市茂园果业专业合作社 | A kind of special pollination liquid of Kiwi berry |
| CN109496834A (en) * | 2018-12-11 | 2019-03-22 | 新疆林业科学院扎木台造林试验站 | A kind of Ba Danmu Liquid pollen and preparation method thereof |
| CN109496834B (en) * | 2018-12-11 | 2022-03-22 | 新疆林业科学院扎木台造林试验站 | Almond liquid pollen and preparation method thereof |
| CN110384112A (en) * | 2019-08-22 | 2019-10-29 | 四川华胜农业股份有限公司 | A kind of Kiwi berry pollination liquid and preparation method and Kiwi berry pollinating method |
| CN111296281A (en) * | 2020-03-31 | 2020-06-19 | 北京成禾佳信农资贸易有限公司 | Pollination method and application thereof |
| CN114521484A (en) * | 2020-11-23 | 2022-05-24 | 成都新朝阳作物科学股份有限公司 | Pollination liquid containing 14-hydroxyl brassinosterol, preparation method and application thereof |
| CN115677404A (en) * | 2022-09-29 | 2023-02-03 | 贵州省果树科学研究所(贵州省柑橘研究所、贵州省特色果蔬工程技术中心、贵州省火龙果研究所) | Preparation and application method of pollen nutrient solution for kiwi pollination |
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