CN104381122B - A kind of Fructus actinidiae chinensis pollination liquid and preparation method thereof - Google Patents
A kind of Fructus actinidiae chinensis pollination liquid and preparation method thereof Download PDFInfo
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- CN104381122B CN104381122B CN201410698821.7A CN201410698821A CN104381122B CN 104381122 B CN104381122 B CN 104381122B CN 201410698821 A CN201410698821 A CN 201410698821A CN 104381122 B CN104381122 B CN 104381122B
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Abstract
The invention discloses a kind of Fructus actinidiae chinensis pollination liquid and preparation method thereof, the component of this pollination liquid include sucrose that percentage by weight is 0.05～0.5%, 0.05～0.5% boric acid and 98.84～99.84% water, also include the vitamin B that percentage by weight is 0.02～0.06%1, 0.02～0.06% vitamin B2, 0.02～0.06% vitamin B3. During preparation, per distribution ratio ratio weighs sucrose, boric acid, vitamin B by weight1, vitamin B2, vitamin B3; After being sufficiently mixed by the component substances weighed, per distribution ratio ratio addition water fully dissolves mixing by weight again, is finally sealed by the homomixture after dissolving and preserves. Add male flower pollen during use and carry out spraying pollination in orchard. The concentration of dusting of the Fructus actinidiae chinensis pollination liquid of the present invention is accurate, female flower fruit-setting rate is high, pollination speed is fast, rate is low, the effect of protective yield of guaranteeing the quality is good for deformed fruit fruit.
Description
Technical field
The present invention relates to technique for culturing yangtao field, especially Fructus actinidiae chinensis spraying pollination technique field, specifically a kind of Fructus actinidiae chinensis pollination liquid and preparation method thereof.
Background technology
Fructus actinidiae chinensis, also referred to as fox Fructus Persicae, Radix actinidiae argutae, Fructus actinidiae chinensis, carambola, wood, Mao Muguo, kiwi fruit, MATENG fruit etc., is distributed widely between the southern china mountain ridge. Fructus actinidiae chinensis is dioecious large-scale fallen leaves bejuco. Staminiferous plant hirsutism leaf is little, and staminiferous plant also relatively early comes across female flower; Female plant lacks hair or without hair, floral leaf is all higher than staminiferous plant. Florescence was 5 June, and fructescence was 8 October.
Under field conditions (factors), mainly by insect pollination, in the more sufficient orchard fine with management of human resources, artificial supplementary pollination is also one of important agricultural measures to Fructus actinidiae chinensis. Substantial amounts of artificial pollination practice have shown that, better, the more, fruit is bigger, and quality is also good for the seed of fruit in pollination. Along with the expansion of Fructus actinidiae chinensis industry, conventional pollination technique is more and more awkward, and the most of grower in China various places is now mostly is adopt conventional pollinating method (artificial point awards method) and Apis supple-mentary pollination, does not reach the effect that yield is high at all. And, conventional artificial pollination method is owing to being subject to the restriction of many reasons, make the method have the length that expends time in, expend material resources greatly, expend the shortcomings such as pollen is many, this results in the development of Fructus actinidiae chinensis industry and be greatly affected by restriction, and make many investor inspire awe even from distance.
The spraying pollination method that research and development pollination liquid carries out Fructus actinidiae chinensis is utilized to can solve this problem, its using method be by pollen be directly added into pollination liquid in shake up, utilizing aerosol apparatus directly female flower to be sprayed, so having dusts concentrates accurately, female flower pollination rate is high, pollination speed is fast, expend time in short, the saving plurality of advantages such as human and material resources and pollen. But, prior art does not still obtain desirable achievement for the research of Fructus actinidiae chinensis pollination liquid.
Summary of the invention
The invention aims to overcome the deficiencies in the prior art, a kind of Fructus actinidiae chinensis pollination liquid and preparation method thereof is provided, this pollination liquid be capable of dusting concentration accurately, female flower fruit-setting rate is high, pollination speed is fast, rate is low, the effect of protective yield of guaranteeing the quality is good for deformed fruit fruit, save the advantage such as human and material resources and pollen.
The present invention is by the following technical programs to realize this purpose:
First, the present invention proposes a kind of Fructus actinidiae chinensis pollination liquid, this pollination liquid is for carrying out spraying pollination by aerosol apparatus after mixing with Fructus actinidiae chinensis male flower pollen, wherein, the component of this pollination liquid include sucrose that percentage by weight is 0.05～0.5%, 0.05～0.5% boric acid and 98.84～99.84% water. Further, the component of this pollination liquid also includes the vitamin B that percentage by weight is 0.02～0.06%1, 0.02～0.06% vitamin B2, 0.02～0.06% vitamin B3. Preferably, this pollination liquid is made up of following components in percentage by weight: sucrose 0.2%, boric acid 0.2%, vitamin B10.04%, vitamin B20.04%, vitamin B30.04% and water 99.48%.
Wherein, sucrose described in technical scheme refers to decompose the saccharide of D-Glucose and the D-Fructose producing equivalent, the combination of one or more including white sugar, soft plantation white sugar and brown sugar (Saccharum Sinensis Roxb.) etc.; Described boric acid is the combination of one or more of boric acid, Borax etc.; Indication vitamin B1For water soluble vitamins; Indication vitamin B2For water soluble vitamins; Indication vitamin B3For water soluble vitamins; Described water is the combination of one or more of distilled water, deionized water, high purity water and tap water etc. And then, in the formula of the pollination liquid of the present invention, sucrose can replace with glucose or calcium gluconate, or allied substances replaces, and boric acid can replace with Borax.
The preparation method that the invention allows for this Fructus actinidiae chinensis pollination liquid, the method comprises the steps:
(1) raw material weighing: per distribution ratio ratio weighs sucrose, boric acid, vitamin B by weight1, vitamin B2, vitamin B3; (2) mixing: alleged pollination liquid component substances is sufficiently mixed; (3) dissolve: the water of the required ratio of per distribution ratio ratio addition fully dissolves by weight; (4) preservation is sealed: seal with vial or plastic bottle etc. and preserve.
Wherein, seal preserve reserve temperature be-10 DEG C～30 DEG C, pollination liquid storage pH be 5～7.5, storage light dark, normal light and high light, bottling adopt vial or plastic bottle, clear vial or brown bottle etc.
The pollination liquid of the present invention is when orchard uses, and after liquid of being pollinated by this Kiwi fruit pollen is diluted with water to 200～500 times of original volume, then shakes up by the pollen of the amount addition 0.02g of the pollination every 1000ml of liquid after dilution, now with the current. Spray amount is that every female plant (300～450 female flowers) 40～100ml pollinates liquid (after dilution and add the amount after pollen), and spray timings is 1～2 time, and aerosol apparatus should strictly clean up.
The pollination liquid of the present invention needs to add the pollen of male flower in use, and the collection of male flower is before pollination 1～10 day, selects than the staminiferous plant that the main breed florescence is slightly Zao, pollen amount is many and main breed affinity is strong, pollen germination rate is high, the florescence is long. The collection method of pollen: the male flower just opened without rainwater, dew taken off be placed on kraft paper or device of writing a prescription to divide straticulation and naturally dry in the shade or be placed in temperature and be 20 DEG C～25 DEG C, place diel in the drying baker of humidity 45～55% for 6～10 in the morning, until male flower all becomes very crisp from the inside to surface, then rub on the sieve of 120～200 orders or 1～3mm wire gauze, reject petal and filigree; Or with small-sized electric pulverizer, male flower is pulverized, petal and filigree is rejected after sieve. Pollen storage: collection pollen is loaded in centrifuge tube or bottle and is placed in-25 DEG C～5 DEG C refrigerators preserves.
The present invention is compared with prior art, the concentration of dusting of the Fructus actinidiae chinensis pollination liquid of the present invention is accurate, female flower fruit-setting rate is high, pollination speed is fast, rate is low, the effect of protective yield of guaranteeing the quality is good for deformed fruit fruit, saving human and material resources and pollen etc., and alternative Fructus actinidiae chinensis Traditional Man point awards the Kiwi fruit pollen pollination liquid nebulization of pollination method.
Detailed description of the invention
Below in conjunction with embodiment, the present invention is described in further detail.
Embodiment 1:
Fructus actinidiae chinensis pollination formula of liquid is by sucrose, boric acid, vitamin B1, vitamin B2, vitamin B3Form with water, prepare the Fructus actinidiae chinensis pollination liquid of 1000ml:
(1) prepared by Fructus actinidiae chinensis pollination liquid:
Raw material weighing: per distribution ratio ratio weighs sucrose 2g, boric acid 2g, vitamin B by weight10.4g, vitamin B20.4g, vitamin B30.4g;
Mixing: alleged pollination liquid component substances is sufficiently mixed;
Dissolve: the water of per distribution ratio ratio addition 994.8ml fully dissolves by weight;
Seal and preserve: seal with vial or plastic bottle etc. and preserve.
(2) Fructus actinidiae chinensis pollination liquid uses:
When orchard uses, by above-mentioned Kiwi fruit pollen pollination liquid 200 times of liquid of dilution, namely dilute becomes 200 times that former pollination liquid is long-pending, and the pollen according still further to the amount addition 0.02g of the every 1000ml of liquid after dilution shakes up, now with the current. The Fructus actinidiae chinensis pollination liquid that the following stated is now joined refers both to after dilute and adds the pollination liquid of pollen. Pollination liquid after dilution just can carry out aerosol apparatus spraying and pollinate after adding pollen, spray amount is that every female plant (300～400 female flowers) 40ml pollinates liquid, and spray timings is 1 time, and aerosol apparatus should strictly clean up.
(3) Pollination Effect of pollination liquid measures:
The impact of pollination liquid stability is measured by A, different reserve temperature:
The Fructus actinidiae chinensis pollination liquid now joined is loaded corresponding transparent conical flask with 200ml for standard, is placed on the condition second half year that temperature is 15 DEG C, 25 DEG C, 35 DEG C routine observation record.
The impact of pollination liquid stability is measured by B, different pH:
The Fructus actinidiae chinensis pollination liquid now joined is loaded corresponding transparent conical flask with 200ml for standard, is placed on the condition next month that PH is 5.5,6.0,6.5,7.0 and 7.5 routine observation record.
The impact of pollination liquid stability is measured by C, different illumination:
The Fructus actinidiae chinensis pollination liquid now joined is loaded corresponding transparent conical flask and Brown Glass Brown glass bottles and jars only with 200ml for standard, is placed on the condition next month of high light, normal condition and dark routine observation record.
The impact of pollen germination is measured by D, pollination liquid:
Pollination drop and is spread out on coverslip, pollen is uniformly dispersed on pollination liquid, pollen amount on every kind of pollination liquid should keep roughly the same, coverslip upset is put on concavity slide, around put several wet cotton balls soaking into clear water, and be put in the calorstat that temperature and humidity is identical and cultivate, after 4 hours, examine under a microscope pollen germination rate.
The impact that Fructus actinidiae chinensis is beared fruit by E, pollination liquid measures:
Pollinating after Fructus actinidiae chinensis female flower pollinated by liquid at Fructus actinidiae chinensis, a number of Fructus actinidiae chinensis female flower has been carried out record, after Fructus actinidiae chinensis is thanked and spent, 30 days investigation Fructus actinidiae chinensiss are beared fruit number.
The impact of Fructus actinidiae chinensis yield and quality is measured by F, pollination liquid:
After fruit maturation, gather in the crops by community, measure each community single plant yield, 200 fruits of each community random acquisition, take back experimental determination fruit quality, single fruit weight, transverse diameter and vertical footpath, calculate fruit shape index. Nutrient quality assay method: Soluble adhesion molecule anthrone colorimetry measures; The assay method of dry and moisture in the content of dry GB8858-1988 fruit, victual; Vitamin C content 2,6 dichlorophenol indophenol method measures; Titratable acid content determination of acid-basetitration.
(4) technique effect of embodiment 1:
As shown in table 1, table 2, table 3, wherein table 1 illustrates the Fructus actinidiae chinensis pollination formula of liquid study on the stability result of embodiment 1, table 2 illustrates the Fructus actinidiae chinensis pollination formula of liquid of embodiment 1 and the impact of Kiwi fruit pollen germination rate, fruit-setting rate and malformed fruit rate is investigated result, and table 3 illustrates Fructus actinidiae chinensis pollination formula of liquid and Fructus actinidiae chinensis is protected the guarantee the quality impact of (adopt latter 7 days and measure quality) of product investigates result.
Wherein, liquid that this Fructus actinidiae chinensis is pollinated carries out reserve temperature, pH, illumination investigation (see table 1), and result shows that pollination liquid has good stability. And can draw from table 2 and table 3, compare with clear water pollen liquid, Fructus actinidiae chinensis pollination liquid makes pollen germination rate reach 68.03%, Fructus actinidiae chinensis fruit-setting rate reaches 91.50%, deformed fruit fruit rate is only 3.16%, improve the Vitamin C content of Fructus actinidiae chinensis 14.17%, 39.51% Soluble adhesion molecule, 0.90% dry matter content, reduce the titratable acid content of 8.46%, sugar-acid ratio reaches 8.10, and can improve mean fruit weight 8.46% and improve Fructus actinidiae chinensis fruit shape index; Compared with awarding with artificial point, every quality and yield index content is suitable, and difference is less. To sum up, it is good that pollination rate height is used in this Fructus actinidiae chinensis pollination liquid field, guarantor produces preservation and effect, is applied on Fructus actinidiae chinensis and carries out spraying pollination, quick, efficient, with low cost, can play the effect reducing work workload and production cost.
Certainly, it it is more than the concrete exemplary applications of the present invention, the present invention also has other embodiment, any without departing from technical solution of the present invention content, according to any simple modification, equivalent variations and modification that above example is made by the technical spirit of the present invention, all still fall within the scope of technical solution of the present invention.
Claims (5)
1. a Fructus actinidiae chinensis pollination liquid, this pollination liquid for Fructus actinidiae chinensis male flower pollen mix after by aerosol apparatus carry out spraying pollinate, it is characterised in that: the component that this pollination liquid by following percentage by weight is forms: the sucrose of 0.05～0.5%, the boric acid of 0.05～0.5%, 0.02～0.06% vitamin B1, 0.02～0.06% vitamin B2, 0.02～0.06% vitamin B3, all the other are water.
2. Fructus actinidiae chinensis according to claim 1 pollination liquid, it is characterised in that: this pollination liquid is made up of following components in percentage by weight: sucrose 0.2%, boric acid 0.2%, vitamin B10.04%, vitamin B20.04%, vitamin B30.04% and water 99.48%.
3. Fructus actinidiae chinensis according to claim 2 pollination liquid, it is characterised in that: described sucrose can replace with glucose or calcium gluconate, and described boric acid can replace with Borax.
4. in a claim 1-3 described in any one Fructus actinidiae chinensis pollination liquid preparation method, it is characterised in that the method comprises the steps:
1) raw material weighing: per distribution ratio ratio weighs sucrose, boric acid, vitamin B by weight1, vitamin B2, vitamin B3;
2) mixing: the component substances weighed in step 1) is sufficiently mixed;
3) dissolve: per distribution ratio ratio addition water fully dissolves mixing by weight;
4) preservation is sealed: the homomixture after dissolving is sealed preservation.
5. the preparation method of Fructus actinidiae chinensis according to claim 4 pollination liquid, it is characterised in that: liquid reserve temperature of pollinating when sealing preservation in step 4) is-10 DEG C～25 DEG C, pollination liquid storage pH is 5～7.5.
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| CN105165597A (en) * | 2015-08-07 | 2015-12-23 | 江苏丘陵地区镇江农业科学研究所 | Pollination method for kiwifruit |
| CN105230373A (en) * | 2015-09-30 | 2016-01-13 | 广西南国之丹生态农业专业合作社 | Method for managing trees, flowers and fruits of red heart kiwi berry |
| CN105994266A (en) * | 2015-12-29 | 2016-10-12 | 龙友华 | Actinidia spray pollination liquid and preparation method thereof |
| CN105567783B (en) * | 2016-03-10 | 2019-04-19 | 中国农业科学院特产研究所 | A kind of tara vine pollen storage and in vitro culture vigor testing methods |
| CN106069253B (en) * | 2016-06-13 | 2019-06-07 | 山东梦农农业科技有限公司 | A kind of pollinating method of greenhouse crops |
| CN106106128A (en) * | 2016-06-30 | 2016-11-16 | 固镇县华丰农业有限公司 | A kind of artificial pollination method of Fructus actinidiae chinensis |
| CN107183031A (en) * | 2017-06-04 | 2017-09-22 | 贵州村华秋实现代生态农业有限公司 | A kind of pollination liquid of red kiwifruit spraying pollination method |
| CN107926977A (en) * | 2017-12-06 | 2018-04-20 | 丹阳市茂园果业专业合作社 | A kind of special pollination liquid of Kiwi berry |
| CN107820907A (en) * | 2017-12-13 | 2018-03-23 | 张家界富硒农业发展有限公司 | A kind of method for improving peach fruit-setting rate |
| CN110384112A (en) * | 2019-08-22 | 2019-10-29 | 四川华胜农业股份有限公司 | A kind of Kiwi berry pollination liquid and preparation method and Kiwi berry pollinating method |
| CN115067336A (en) * | 2022-06-13 | 2022-09-20 | 广西壮族自治区中国科学院广西植物研究所 | Pollination liquid for promoting pollen germination of kiwi fruits, preparation method and application thereof |
| CN115677404A (en) * | 2022-09-29 | 2023-02-03 | 贵州省果树科学研究所(贵州省柑橘研究所、贵州省特色果蔬工程技术中心、贵州省火龙果研究所) | Preparation and application method of pollen nutrient solution for kiwi pollination |
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