CN104381122A - Kiwi fruit pollination liquid and preparation method thereof - Google Patents

Kiwi fruit pollination liquid and preparation method thereof Download PDF

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Publication number
CN104381122A
CN104381122A CN201410698821.7A CN201410698821A CN104381122A CN 104381122 A CN104381122 A CN 104381122A CN 201410698821 A CN201410698821 A CN 201410698821A CN 104381122 A CN104381122 A CN 104381122A
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kiwi fruit
pollination
cobastab
pollination liquid
liquid
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CN104381122B (en
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张承
王秋萍
龙友华
李明
常陆枫
尹显慧
吴小毛
胡安龙
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Wuhan Jusen Agricultural Technology Co.,Ltd.
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Guizhou University
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Abstract

The invention discloses a kiwi fruit pollination liquid and a preparation method thereof. The kiwi fruit pollination liquid is prepared from the following components in percentage by weight: 0.05-0.5% of cane sugar, 0.05-0.5% of boric acid, 98.84-99.84% of water as well as 0.02-0.06% of vitamin B1, 0.02-0.06% of vitamin B2 and 0.02-0.06% of vitamin B3. The preparation method comprises the following steps: weighing the cane sugar, the boric acid, the vitamin B1, the vitamin B2 and the vitamin B3 according to the percentages by weight, sufficiently mixing the weighed substances, adding water for sufficiently dissolving and uniformly mixing the components according to the percentage by weight, and finally sealing and storing the dissolved uniform mixture. When the kiwi fruit pollination liquid is used, male flower pollen is added and sprayed to pollinate in fruit gardens. The kiwi fruit pollination liquid disclosed by the invention can be accurately sprayed, the fruit setting rate of female flowers is high, the pollination speed is high, the malformed fruit rate is low, and the quality and yield maintaining effect is good.

Description

A kind of kiwi fruit pollination liquid and preparation method thereof
Technical field
The present invention relates to technique for culturing yangtao field, especially kiwi fruit spraying pollination technique field, specifically a kind of kiwi fruit pollination liquid and preparation method thereof.
Background technology
Kiwi fruit, also claim fox peach, bower actinidia root or leaf, Chinese gooseberry, carambola, wood, Mao Muguo, Chinese grooseberry, numb rattan fruit etc., be distributed widely between the southern china mountain ridge.Kiwi fruit is dioecious large-scale fallen leaves bejuco.Staminiferous plant crinosity leaf is little, and staminiferous plant also comparatively early comes across female flower; Female plant lacks hair or without hair, floral leaf is all greater than staminiferous plant.Florescence is 5-June, and fructescence is 8-October.
Under field conditions (factors), mainly by insect pollination, in and orchard that management is meticulous more sufficient at human resources, artificial supplementary pollination is also one of important agricultural measures to kiwi fruit.A large amount of artificial pollination practices shows, better, the more, fruit is individual larger, and quality is also good for the seed of fruit in pollination.Along with the expansion of kiwi fruit industry, conventional pollination technique is more and more awkward, and the most of grower in China various places is now is mostly adopt conventional pollinating method (artificial point awards method) and honeybee supplementary pollination, does not reach the effect that output is high at all.And, conventional artificial pollination method is owing to being subject to the restriction of many reasons, the method is had expend time in long, expend material resources large, expend the shortcomings such as pollen is many, the development that this results in kiwi fruit industry is restricted greatly, and many investors are inspired awe even from distance.
The spraying pollination method utilizing research and development pollination liquid to carry out kiwi fruit can solve this problem, its using method shakes up by pollen directly being added in pollination liquid, utilize sprayer directly to spray to female flower, having like this dusts concentrates accurately, female flower pollination rate is high, pollination speed fast, it is short to expend time in, save the plurality of advantages such as human and material resources and pollen.But, in prior art, desirable achievement is not still obtained for the research of kiwi fruit pollination liquid.
Summary of the invention
The object of the invention is to overcome the deficiencies in the prior art, there is provided a kind of kiwi fruit to pollinate liquid and preparation method thereof, this pollination liquid can realize dusting concentrate accurately, female flower fruit-setting rate is high, pollination speed fast, deformed fruit fruit rate is low, the effect of protective yield of guaranteeing the quality is good, save the advantage such as human and material resources and pollen.
The present invention is by the following technical programs to realize this object:
First, the present invention proposes a kind of kiwi fruit pollination liquid, carry out spraying by sprayer and pollinate after this pollination liquid is used for mixing with kiwi fruit male flower pollen, wherein, the component of this pollination liquid comprise percentage by weight be the sucrose of 0.05 ~ 0.5%, the boric acid of 0.05 ~ 0.5% and 98.84 ~ 99.84% water.Further, the Cobastab that percentage by weight is 0.02 ~ 0.06% is also comprised in the component of this pollination liquid 1, 0.02 ~ 0.06% Cobastab 2, 0.02 ~ 0.06% Cobastab 3.Preferably, this pollination liquid is made up of the component of following percentage by weight: sucrose 0.2%, boric acid 0.2%, Cobastab 10.04%, Cobastab 20.04%, Cobastab 30.04% and water 99.48%.
Wherein, sucrose described in technical scheme of the present invention refers to decompose and produces the D-Glucose of equivalent and the carbohydrate of D-Fructose, comprises the combination of one or more of white granulated sugar, soft white sugar and brown granulated sugar etc.; Described boric acid is the combination of one or more of boric acid, borax etc.; Indication Cobastab 1for water soluble vitamin; Indication Cobastab 2for water soluble vitamin; Indication Cobastab 3for water soluble vitamin; Described water is the combination of one or more of distilled water, deionized water, high purity water and running water etc.And then, in the formula of pollination liquid of the present invention, sucrose can replace with glucose or calcium gluconae, or allied substances replaces, and boric acid can replace with borax.
The invention allows for the preparation method of this kiwi fruit pollination liquid, the method comprises the steps:
(1) raw material weighing: per distribution ratio ratio takes sucrose, boric acid, Cobastab by weight 1, Cobastab 2, Cobastab 3; (2) mix: alleged pollination liquid component substances is fully mixed; (3) dissolve: the water that per distribution ratio ratio adds required ratio by weight fully dissolves; (4) sealing is preserved: preserve with the sealing such as vial or plastic bottle.
Wherein, sealing preserves that reserve temperature be-10 DEG C ~ 30 DEG C, pollination liquid storage pH is 5 ~ 7.5, storage light dark, normal light and high light, bottling employing vial or plastic bottle, clear vial or brown bottle etc.
Pollination liquid of the present invention, when orchard uses, after this Kiwi fruit pollen pollination liquid is diluted with water to 200 ~ 500 times of original volume, then shakes up by the pollen that the amount of the every 1000ml of pollination liquid after dilution adds 0.02g, now with the current.Spray amount is every female plant (300 ~ 450 female flowers) 40 ~ 100ml pollination liquid (diluting rear and after adding pollen amount), and spray timings is 1 ~ 2 time, and sprayer should strictly clean up.
Pollination liquid of the present invention needs the pollen adding male flower in use, and the collection of male flower is before pollination 1 ~ 10 day, the Selection radio main breed florescence slightly early, pollen amount is many, with the staminiferous plant that main breed affinity is strong, pollen germination rate is high, the florescence is long.The collection method of pollen: the male flower just opened not containing rainwater, dew taken off be placed on brown paper or straticulation divided by device of writing a prescription and naturally dries in the shade or be placed in that temperature is 20 DEG C ~ 25 DEG C, diel placed by the drying box of humidity 45 ~ 55% for 6 ~ 10 in the morning, until male flower all becomes very crisp from the inside to surface, then rub on 120 ~ 200 object sieves or 1 ~ 3 mm wire netting, reject petal and filigree; Or with small-sized electric cracker, male flower is pulverized, reject petal and filigree after sieve.Pollen storage: collection pollen is loaded in centrifuge tube or bottle and is placed in-25 DEG C ~ 5 DEG C refrigerators and preserves.
The present invention compared with prior art, dusting of kiwi fruit pollination liquid of the present invention is concentrated accurately, female flower fruit-setting rate is high, pollination speed fast, deformed fruit fruit rate is low, the effect of protective yield of guaranteeing the quality is good, save human and material resources and pollen etc., and alternative kiwi fruit Traditional Man point awards the Kiwi fruit pollen pollination liquid spray-on process of pollination method.
Embodiment
Below in conjunction with embodiment, the present invention is described in further detail.
Embodiment 1:
Kiwi fruit pollination formula of liquid is by sucrose, boric acid, Cobastab 1, Cobastab 2, Cobastab 3with water composition, the kiwi fruit pollination liquid of preparation 1000ml:
(1) kiwi fruit pollination liquid preparation:
Raw material weighing: per distribution ratio ratio takes sucrose 2g, boric acid 2g, Cobastab by weight 10.4g, Cobastab 20.4g, Cobastab 30.4g;
Mixing: alleged pollination liquid component substances is fully mixed;
Dissolve: the water that per distribution ratio ratio adds 994.8ml by weight fully dissolves;
Sealing is preserved: preserve with the sealing such as vial or plastic bottle.
(2) kiwi fruit pollination liquid uses:
When orchard uses, above-mentioned Kiwi fruit pollen pollination liquid is diluted 200 times of liquid, i.e. thin up 200 times of becoming former pollination liquid long-pending, then shake up according to the pollen that the amount of the every 1000ml of liquid after dilution adds 0.02g, now with the current.The kiwi fruit pollination liquid that the following stated is now joined all to refer to after thin up and to add the pollination liquid of pollen.Pollination liquid after dilution just can carry out sprayer spraying pollination after adding pollen, spray amount is that every female plant (300 ~ 400 female flowers) 40ml pollinates liquid, and spray timings is 1 time, and sprayer should strictly clean up.
(3) Pollination Effect of pollination liquid measures:
The impact on pollination liquid stability of A, different reserve temperature measures:
Liquid of being pollinated by the kiwi fruit of now joining is that standard loads corresponding transparent conical flask with 200ml, is placed on the condition second half year that temperature is 15 DEG C, 25 DEG C, 35 DEG C, and routine observation record.
B, the different pH impact on pollination liquid stability measures:
Liquid of being pollinated by the kiwi fruit of now joining is that standard loads corresponding transparent conical flask with 200ml, is placed on the condition next month that PH is 5.5,6.0,6.5,7.0 and 7.5, and routine observation record.
The impact on pollination liquid stability of C, different light measures:
Liquid of being pollinated by the kiwi fruit of now joining is that standard loads corresponding transparent conical flask and Brown Glass Brown glass bottles and jars only with 200ml, is placed on the condition next month of high light, normal condition and dark, and routine observation record.
D, pollination liquid measure the impact of pollen germination:
Pollination drop is spread out on cover glass, pollen is evenly dispersed on pollination liquid, pollen amount on often kind of pollination liquid should keep roughly the same, cover glass upset is put on concavity slide, put several wet cotton balls soaking into clear water around, and be put in the identical insulating box of temperature and humidity and cultivate, after 4 hours, examine under a microscope pollen germination rate.
E, pollination liquid measure the impact that kiwi fruit is beared fruit:
After liquid pollinates to kiwi fruit female flower in kiwi fruit pollination, record has been carried out to a number of kiwi fruit female flower, kiwi fruit thank spend after 30 days investigation kiwi fruits to bear fruit number.
F, pollination liquid measure the impact of kiwi fruit Yield and qualities:
After fruit maturation, by community results, measure each community single plant yield, each community random acquisition 200 fruits, take back experimental determination fruit quality, single fruit weight, transverse diameter and vertical footpath, calculate fruit shape index.Nutrient quality assay method: Soluble adhesion molecule anthrone colorimetry measures; The assay method of dry matter and moisture in the content GB8858-1988 fruit of dry matter, victual; Vitamin C content 2,6-dichloroindophenol method measures; Titratable acid content determination of acid-basetitration.
(4) technique effect of embodiment 1:
As shown in table 1, table 2, table 3, wherein table 1 illustrates the kiwi fruit pollination formula of liquid study on the stability result of embodiment 1, the kiwi fruit pollination formula of liquid that table 2 illustrates embodiment 1 investigates result to the impact of Kiwi fruit pollen germination rate, fruit-setting rate and malformed fruit rate, and table 3 illustrates kiwi fruit pollination formula of liquid and protects the guarantee the quality impact of (adopt and measure quality in latter 7 days) of product to kiwi fruit and investigate result.
Wherein, carry out reserve temperature, pH, illumination investigation (see table 1) to this kiwi fruit pollination liquid, result shows that pollination liquid has good stability.And can draw from table 2 and table 3, compare with clear water pollen liquid, kiwi fruit pollination liquid makes pollen germination rate reach 68.03%, kiwi fruit fruit-setting rate reaches 91.50%, deformed fruit fruit rate is only 3.16%, improve the Vitamin C content of kiwi fruit 14.17%, 39.51% Soluble adhesion molecule, the dry matter content of 0.90%, the titratable acid content, the sugar-acid ratio that reduce by 8.46% reach 8.10, and mean fruit weight 8.46% can be improved and improve kiwi fruit fruit shape index; Compared with awarding with artificial point, every quality and yield index content is suitable, and difference is less.To sum up, this kiwi fruit pollination liquid field use pollination rate high, protect that to produce preservation and effect good, be applied to and kiwi fruit carry out spraying pollination, quick, efficient, with low cost, the effect of reduction work workload and production cost can be played.
Certainly, it is more than embody rule example of the present invention, the present invention also has other embodiment, anyly do not depart from technical solution of the present invention content, the any simple modification done above embodiment according to technical spirit of the present invention, equivalent variations and modification, all still belong in the scope of technical solution of the present invention.

Claims (6)

1. a kiwi fruit pollination liquid, carry out spraying by sprayer and pollinate after this pollination liquid is used for mixing with kiwi fruit male flower pollen, it is characterized in that: the component of this pollination liquid comprise percentage by weight be the sucrose of 0.05 ~ 0.5%, the boric acid of 0.05 ~ 0.5% and 98.84 ~ 99.84% water.
2. kiwi fruit pollination liquid according to claim 1, is characterized in that: also comprise the Cobastab that percentage by weight is 0.02 ~ 0.06% in the component of this pollination liquid 1, 0.02 ~ 0.06% Cobastab 2, 0.02 ~ 0.06% Cobastab 3.
3. kiwi fruit pollination liquid according to claim 2, is characterized in that: this pollination liquid is made up of the component of following percentage by weight: sucrose 0.2%, boric acid 0.2%, Cobastab 10.04%, Cobastab 20.04%, Cobastab 30.04% and water 99.48%.
4. kiwi fruit pollination liquid according to claim 3, is characterized in that: described sucrose can replace with glucose or calcium gluconae, and described boric acid can replace with borax.
5. a preparation method for the kiwi fruit pollination liquid as described in above-mentioned any claim, is characterized in that the method comprises the steps:
1) raw material weighing: per distribution ratio ratio takes sucrose, boric acid, Cobastab by weight 1, Cobastab 2, Cobastab 3;
2) mix: the component substances taken in step 1) is fully mixed;
3) dissolve: per distribution ratio ratio adds water and fully dissolves mixing by weight;
4) sealing is preserved: the homomixture sealing after dissolving preserved.
6. the preparation method of kiwi fruit pollination liquid according to claim 5, is characterized in that: liquid reserve temperature of pollinating when sealing is preserved in step 4) is-10 DEG C ~ 25 DEG C, pollination liquid storage pH is 5 ~ 7.5.
CN201410698821.7A 2014-11-28 2014-11-28 A kind of Fructus actinidiae chinensis pollination liquid and preparation method thereof Active CN104381122B (en)

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Cited By (12)

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CN105165597A (en) * 2015-08-07 2015-12-23 江苏丘陵地区镇江农业科学研究所 Pollination method for kiwifruit
CN105230373A (en) * 2015-09-30 2016-01-13 广西南国之丹生态农业专业合作社 Method for managing trees, flowers and fruits of red heart kiwi berry
CN105567783A (en) * 2016-03-10 2016-05-11 中国农业科学院特产研究所 Preservation and in-vitro culture vitality detection method for pollen of Actinidia arguta
CN105994266A (en) * 2015-12-29 2016-10-12 龙友华 Actinidia spray pollination liquid and preparation method thereof
CN106069253A (en) * 2016-06-13 2016-11-09 山东梦农农业科技有限公司 A kind of pollinating method of greenhouse crops
CN106106128A (en) * 2016-06-30 2016-11-16 固镇县华丰农业有限公司 A kind of artificial pollination method of Fructus actinidiae chinensis
CN107183031A (en) * 2017-06-04 2017-09-22 贵州村华秋实现代生态农业有限公司 A kind of pollination liquid of red kiwifruit spraying pollination method
CN107820907A (en) * 2017-12-13 2018-03-23 张家界富硒农业发展有限公司 A kind of method for improving peach fruit-setting rate
CN107926977A (en) * 2017-12-06 2018-04-20 丹阳市茂园果业专业合作社 A kind of special pollination liquid of Kiwi berry
CN110384112A (en) * 2019-08-22 2019-10-29 四川华胜农业股份有限公司 A kind of Kiwi berry pollination liquid and preparation method and Kiwi berry pollinating method
CN115067336A (en) * 2022-06-13 2022-09-20 广西壮族自治区中国科学院广西植物研究所 Pollination liquid for promoting pollen germination of kiwi fruits, preparation method and application thereof
CN115677404A (en) * 2022-09-29 2023-02-03 贵州省果树科学研究所(贵州省柑橘研究所、贵州省特色果蔬工程技术中心、贵州省火龙果研究所) Preparation and application method of pollen nutrient solution for kiwi pollination

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Cited By (15)

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Publication number Priority date Publication date Assignee Title
CN105165597A (en) * 2015-08-07 2015-12-23 江苏丘陵地区镇江农业科学研究所 Pollination method for kiwifruit
CN105230373A (en) * 2015-09-30 2016-01-13 广西南国之丹生态农业专业合作社 Method for managing trees, flowers and fruits of red heart kiwi berry
CN105994266A (en) * 2015-12-29 2016-10-12 龙友华 Actinidia spray pollination liquid and preparation method thereof
CN105567783B (en) * 2016-03-10 2019-04-19 中国农业科学院特产研究所 A kind of tara vine pollen storage and in vitro culture vigor testing methods
CN105567783A (en) * 2016-03-10 2016-05-11 中国农业科学院特产研究所 Preservation and in-vitro culture vitality detection method for pollen of Actinidia arguta
CN106069253A (en) * 2016-06-13 2016-11-09 山东梦农农业科技有限公司 A kind of pollinating method of greenhouse crops
CN106069253B (en) * 2016-06-13 2019-06-07 山东梦农农业科技有限公司 A kind of pollinating method of greenhouse crops
CN106106128A (en) * 2016-06-30 2016-11-16 固镇县华丰农业有限公司 A kind of artificial pollination method of Fructus actinidiae chinensis
CN107183031A (en) * 2017-06-04 2017-09-22 贵州村华秋实现代生态农业有限公司 A kind of pollination liquid of red kiwifruit spraying pollination method
CN107926977A (en) * 2017-12-06 2018-04-20 丹阳市茂园果业专业合作社 A kind of special pollination liquid of Kiwi berry
CN107820907A (en) * 2017-12-13 2018-03-23 张家界富硒农业发展有限公司 A kind of method for improving peach fruit-setting rate
CN110384112A (en) * 2019-08-22 2019-10-29 四川华胜农业股份有限公司 A kind of Kiwi berry pollination liquid and preparation method and Kiwi berry pollinating method
CN115067336A (en) * 2022-06-13 2022-09-20 广西壮族自治区中国科学院广西植物研究所 Pollination liquid for promoting pollen germination of kiwi fruits, preparation method and application thereof
CN115067336B (en) * 2022-06-13 2024-09-17 广西壮族自治区中国科学院广西植物研究所 Pollination liquid for promoting germination of kiwi fruit pollen, preparation method and application thereof
CN115677404A (en) * 2022-09-29 2023-02-03 贵州省果树科学研究所(贵州省柑橘研究所、贵州省特色果蔬工程技术中心、贵州省火龙果研究所) Preparation and application method of pollen nutrient solution for kiwi pollination

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