[ summary of the invention ]
Aiming at the problems, the technical problem to be solved by the invention is to provide a method for promoting the fruiting of the winter tips of the woolly oranges, the winter tips treated by the method are robust, thick in leaves, fast in green turning, fast in aging and normal in flower bud differentiation, and can normally fruit as the autumn happens slightly in the next year.
In order to solve the technical problems, the technical scheme adopted by the invention is as follows:
a method of promoting wakame winter shoot fruiting, the method comprising: spraying a potassium nitrate solution to the surface of the winter tip till the surface is wet in 12-month middle ten days, spraying a mixed liquid medicine to the surface of the winter tip till the surface is dry, and spraying the potassium nitrate solution and the mixed liquid medicine once every 10-15 days for 3 times; the mixed liquid medicine is mainly prepared by mixing organic polypeptidase, amino-oligosaccharin and water according to the weight ratio of 0.4:0.2: 600-800.
Further, the concentration of the potassium nitrate solution is 0.5-1.5%.
Further, the method further comprises the step of spraying an antifreezing agent to the whole crown of the Or after the mixed liquid medicine is used for 1-3 days each time, wherein the antifreezing agent comprises the following raw materials in parts by weight: 3-7 parts of dwarf lilyturf tuber extract, 0.2-0.8 part of soybean polypeptide, 0.5-1 part of alanine, 0.3-0.8 part of cysteine, 2-5 parts of monopotassium phosphate and 800 parts of water.
Further, the preparation method of the radix ophiopogonis extract comprises the following steps: pulverizing dried radix Ophiopogonis tuber, adding 6-10 weight times of water, leaching at 80-90 deg.C in water bath for 1-2 hr, centrifuging, filtering to obtain filtrate, concentrating the filtrate to 1/3, adding 90-95% ethanol solution, standing to precipitate, filtering to obtain precipitate, and drying.
Further, the method also comprises the step of spraying the insecticide once every 20 to 25 days from 12 middle of the month and continuously spraying for 2 to 3 times, wherein the insecticide is imidacloprid or abamectin, and the insecticide is diluted by 1000-1500 times with water before use.
Further, the spraying of the potassium nitrate solution and the mixed liquid medicine is carried out in the evening of a sunny day or in a cloudy day.
The organic polypeptidase and the amino-oligosaccharin can be obtained from the market.
Compared with the prior art, the invention has the following beneficial effects:
the potassium nitrate solution has the effects of promoting stomata of the winter tip leaves to open, increasing the absorption rate of the mixed liquid medicine and providing nutrient components; the organic polypeptide enzyme can activate various enzymes of plants, promote the rapid absorption of nutrients and enhance the anabolic capacity of the plants; the amino-oligosaccharin is rich in carbon and nitrogen, and can provide nutrients, activate cells and enhance stress resistance. The method comprises the steps of firstly treating the leaves with a potassium nitrate solution to open pores of the leaves, then spraying organic polypeptidase and base oligosaccharins, activating cells and various enzymes of the young shoots, enabling anabolism to be vigorous, enabling nutrients to rapidly enter plant cells through the pores and synthesize cell substances, further promoting lengthening and thickening growth of the young shoots, enabling the leaves to become thick and green, achieving the purpose of rapid aging, and meanwhile accumulating sufficient nutrients in the winter shoots along with enhancement of photosynthesis and accumulation of nutrients to prepare for flower bud differentiation in the coming year. After the method is used for processing, the summer oranges can quickly accumulate nutrients on winter tips, the branches are robust, the leaves are thick, the green turning is quick, the aging is quick, the formation of flower buds is facilitated, and the winter oranges can normally bloom and fruit as the autumn tips.
The radix ophiopogonis extract in the antifreezing agent is rich in polysaccharide and organic matters; the soybean polypeptide contains free amino acids, saccharides and inorganic salt components, and has water absorption; the compound amino acid, the cysteine and the potassium dihydrogen phosphate act together to supplement nutrition for plants, improve the concentration of cell sap, form a protective film, prevent blades from being frozen, improve the stress resistance of the whole citrus reiliana plants and provide guarantee for winter shoot aging.
[ detailed description ] embodiments
The following examples are provided to further illustrate the embodiments of the present invention.
The organic polypeptidase and the amino-oligosaccharin of the following examples were obtained commercially.
Example 1
The present embodiment provides a method for promoting winter shoot fruiting in citrus plants, the method comprising: spraying a potassium nitrate solution with the concentration of 0.5% to the surface of the winter tip in 12-month middle ten days till the surface is wet, spraying a mixed liquid medicine to the surface of the winter tip after the surface is dry, and spraying the potassium nitrate solution and the mixed liquid medicine once every 10 days for 3 times; the mixed liquid medicine is mainly prepared by mixing organic polypeptidase, amino-oligosaccharin and water in a weight ratio of 0.4:0.2: 600. The potassium nitrate solution and the mixed liquid medicine are sprayed in the evening of a sunny day.
After the mixed liquid medicine is used for 1 day each time, spraying an antifreezing agent to the whole crown of the Or, wherein the antifreezing agent comprises the following raw materials in parts by weight: 3 parts of dwarf lilyturf tuber extract, 0.2 part of soybean polypeptide, 0.5 part of alanine, 0.3 part of cysteine, 2 parts of monopotassium phosphate and 500 parts of water. The preparation method of the radix ophiopogonis extract comprises the following steps: pulverizing dried radix Ophiopogonis tuber, adding into 6 weight times of water, leaching at 80 deg.C in water bath for 1 hr, centrifuging, filtering to obtain filtrate, concentrating the filtrate to 1/3 of original volume, adding into 90% ethanol solution, standing until precipitate is separated out, filtering to obtain precipitate, and drying.
Spraying insecticide once every 20 days from 12 middle of the month, continuously spraying for 2 times, wherein the insecticide is imidacloprid, and diluting with water 1000 times before use.
Example 2
The present embodiment provides a method for promoting winter shoot fruiting in citrus plants, the method comprising: spraying a potassium nitrate solution with the concentration of 1.5% to the surface of the winter tip in the middle ten days of 12 months until the surface is wet, spraying a mixed liquid medicine to the surface of the winter tip after the surface is dry, and spraying the potassium nitrate solution and the mixed liquid medicine once every 15 days for 3 times; the mixed liquid medicine is mainly prepared by mixing organic polypeptidase, amino-oligosaccharin and water in a weight ratio of 0.4:0.2: 800. The spraying of the potassium nitrate solution and the mixed liquid medicine is carried out in cloudy days.
After the mixed liquid medicine is used for 3 days each time, spraying an antifreezing agent to the whole crown of the Or, wherein the antifreezing agent comprises the following raw materials in parts by weight: 7 parts of radix ophiopogonis extract, 0.8 part of soybean polypeptide, 1 part of alanine, 0.8 part of cysteine, 5 parts of potassium dihydrogen phosphate and 800 parts of water. The preparation method of the radix ophiopogonis extract comprises the following steps: pulverizing dried radix Ophiopogonis tuber, adding 10 weight times of water, leaching in water bath at 90 deg.C for 2 hr, centrifuging, filtering to obtain filtrate, concentrating the filtrate to 1/3 of original volume, adding 95% ethanol solution, standing to precipitate, filtering to obtain precipitate, and drying.
Spraying the insecticide once every 25 days from 12 middle-month ten days, continuously spraying for 3 times, wherein the insecticide is abamectin, and diluting with water by 1500 times before use.
Example 3
The present embodiment provides a method for promoting winter shoot fruiting in citrus plants, the method comprising: spraying a potassium nitrate solution with the concentration of 1.0% to the surface of the winter tip in the middle ten days of 12 months until the surface is wet, spraying a mixed liquid medicine to the surface of the winter tip after the surface is dry, and spraying the potassium nitrate solution and the mixed liquid medicine once every 13 days for 3 times; the mixed liquid medicine is mainly prepared by mixing organic polypeptidase, amino-oligosaccharin and water in a weight ratio of 0.4:0.2: 700. The potassium nitrate solution and the mixed liquid medicine are sprayed in the evening of a sunny day.
After the mixed liquid medicine is used for 2 days each time, spraying an antifreezing agent to the whole crown of the Or, wherein the antifreezing agent comprises the following raw materials in parts by weight: 5 parts of dwarf lilyturf tuber extract, 0.7 part of soybean polypeptide, 0.6 part of alanine, 0.5 part of cysteine, 3 parts of monopotassium phosphate and 600 parts of water. The preparation method of the radix ophiopogonis extract comprises the following steps: pulverizing dried radix Ophiopogonis tuber, adding 7 weight times of water, leaching at 85 deg.C in water bath for 1.5 hr, centrifuging, filtering to obtain filtrate, concentrating the filtrate to 1/3 of original volume, adding into 92% ethanol solution, standing until precipitate is separated out, filtering to obtain precipitate, and drying.
Spraying insecticide once every 22 days from 12 middle of the month, continuously spraying for 3 times, wherein the insecticide is imidacloprid, and diluting with water 1200 times before use.
The method for promoting the winter tip aging effect test of the Or:
test site: guangxi Hezhou Fuchuan county, Majiling town.
Test time: 2016.
Test subjects: 4-year-old Or 80-year-old Citrus reticulata planted in the same field.
Test protocol: the test is provided with 9 treatments, namely a autumn shoot group, CK, A, B, C, D, E, F and G, wherein 10 plants are treated respectively, the autumn shoot group does not carry out any treatment, and the CK carries out no treatment on winter shoots; a, treating the winter shoots by adopting the method of embodiment 3; b, accelerating the winter shoots to ripen by adopting a traditional method, namely spraying a hymenium biostimulant and a phosphorus-potassium source library on the leaf surfaces; c, the winter shoots are not treated by the potassium nitrate solution, and other steps and methods are the same as those of the example 3; d, replacing the organic polypeptidase with an equal amount of water, and performing the same other steps and methods as in example 3; e, replacing the amino-oligosaccharin with an equal amount of water, and performing other steps and methods in the same manner as in example 3; f, no antifreezing agent is sprayed, and other steps and methods are the same as those of the example 3; g, replacing the antifreeze of the application with a commercial fruit and vegetable antifreeze, and carrying out the same other steps and methods as those of the example 3. The other management conditions of each group are consistent.
And (3) test results:
(1) measuring the total length, the diameter and the dry weight of single leaves of each group of branches beginning in the middle ten days of 3 months in 2017, wherein the total length, the diameter and the dry weight of single leaves of autumn tips are measured by the autumn tip group, 3 plants which are consistent in shape and size and grow healthily are selected, 15 autumn tips are randomly selected from each plant for measurement, 30 autumn tip leaves which are basically consistent in size are randomly selected from each plant and dried until the water content is 20%, then the dry weight is weighed by balance, and the color of the autumn tip leaves is visually observed; and CK, A, B, C, D, E, F and G are each group to measure the total length, diameter and single leaf dry weight of the winter tip, each group selects 3 plants with consistent shape and size, basically consistent number of the winter tips and healthy growth, randomly selects 15 branches of the winter tips on each plant to measure, randomly selects 30 leaves of the winter tips with basically consistent size from each plant, dries until the water content is 20%, and then weighs the dry weight with balance to visually observe the color of the leaves of the winter tips. The total length is measured by a tape measure, and the diameter is measured by a vernier caliper; the measurements were counted and the average total length, average diameter and average single leaf dry weight were calculated and the results are shown in table 1.
TABLE 1 average Total winter shoot Length, average diameter and Dry weight of Single leaf for each group
Treatment of
|
Total length (cm)
|
Diameter (cm)
|
Single leaf Dry weight (g)
|
Leaf color
|
Autumn tip group
|
37.1a
|
0.877a
|
0.145a
|
Dark green
|
CK
|
13.4c
|
0.218c
|
0.085c
|
Light yellow
|
A
|
36.6a
|
0.882a
|
0.146a
|
Dark green
|
B
|
19.6b
|
0.407b
|
0.091b
|
Green colour
|
C
|
31.1b
|
0.420b
|
0.122b
|
Green colour
|
D
|
31.5b
|
0.431b
|
0.126b
|
Green colour
|
E
|
32.3b
|
0.433b
|
0.127b
|
Green colour
|
F
|
32.8b
|
0.439b
|
0.135b
|
Green colour
|
G
|
30.7b
|
0.411b
|
0.120b
|
Green colour |
(remarks: a, b, c different letters indicate that the difference reaches a significant level of 5%)
From table 1, it can be seen that: the average total length, average diameter and single leaf dry weight of winter tips of A (the method) are obviously higher than CK (blank) and B (the traditional method), so that after the winter tips are treated by the method, the winter tips of the Or are quickly accumulated in nutrients, strong in branches, thick in leaves, quick in green turning and quick in aging, and the formation of flower buds in the next year is facilitated. According to the comparison between C, D, E, F, G treatment and A treatment, the potassium nitrate solution, the organic polypeptidase, the oligosaccharins and the antifreezing agent act together, have synergistic effect and are inseparable, so that the lengthening and thickening growth of new shoots are promoted together, the thickness of leaves is increased, the leaves turn green, and the purpose of rapid aging is achieved; as can be seen from the comparison of the autumn shoot group with the A group, the average total length, average diameter and dry weight of single leaf of the winter shoot of the treatment method of the invention are basically consistent with the autumn shoot.
(2) In 2017, the citrus reticulata blossoms to the fructification period, 3 plants which are consistent in shape and size, grow healthily and record the position of a winter tip are selected from each group, the fruit rate is observed and counted in the full-bloom period and the fruit period, the fruit rate (%) is the number of fruits per the number of blossoms, the number of blossoms is observed in the remaining period of blossoms, the number of fruits is observed after the fruit drop in the second physiology, the sugar content is measured by using a handheld sugar content measuring instrument, and the results are shown in table 2.
Table 2 shows the winter shoot yield, fruit transverse diameter, fruit longitudinal diameter, single fruit weight and sugar content of each group
(remarks: a, b, c different letters indicate that the difference reaches a significant level of 5%)
From table 2, the winter top yield and the sugar content of the A (the method of the invention) are both significantly high by CK (blank), B (the traditional method), C, D, E, F and G, so that the winter top can fruit in the next year after being treated by the method of the invention, the fruit quality is equivalent to that of the fruit bearing in the next year after being slightly fertilized by the conventional summer citrus (the sugar content of the fruit bearing in the conventional summer citrus and the autumn is 13% -15%), and the comparison between the autumn top group and the A (the method of the invention) shows that the treatment method of the invention not only can enable the winter top to normally bloom and fruit bearing in the next year as well as the autumn top, but also has no significant difference between the fruit quality such as the yield, the average single fruit weight, the sugar content and the like and the autumn top.
The above description is intended to describe in detail the preferred embodiments of the present invention, but the embodiments are not intended to limit the scope of the claims of the present invention, and all equivalent changes and modifications made within the technical spirit of the present invention should fall within the scope of the claims of the present invention.