CN110915563B

CN110915563B - Application of cytokinin 6-BA in prevention and treatment of high-temperature heat damage of anoectochilus formosanus

Info

Publication number: CN110915563B
Application number: CN201911083901.0A
Authority: CN
Inventors: 梅瑜; 王继华
Original assignee: CROP Research Institute of Guangdong Academy of Agricultural Sciences
Current assignee: CROP Research Institute of Guangdong Academy of Agricultural Sciences
Priority date: 2019-11-07
Filing date: 2019-11-07
Publication date: 2022-03-22
Anticipated expiration: 2039-11-07
Also published as: CN110915563A

Abstract

An application of cytokinin 6-BA in preventing and treating high-temperature heat injury of anoectochilus formosanus is characterized in that 6-BA with the concentration of 0.1-1.0 g/L is used for carrying out one-time spraying or multiple-time spraying on the anoectochilus formosanus plants which generate heat injury, and when the 6-BA is sprayed for multiple times, the 6-BA is sprayed for one time every 2-7 days, and the spraying is carried out for 2-5 times in total. Application of cytokinin 6-BA in preparing a prevention and treatment medicament for high-temperature heat damage of anoectochilus formosanus. According to the invention, the Anoectochilus roxburghii plant is sprayed with the 6-BA solution, so that the Anoectochilus roxburghii plant can grow new buds and rejuvenate. The 6-BA solution disclosed by the invention can achieve a good rejuvenation effect on a plurality of anoectochilus formosanus varieties. The 6-BA solution is sprayed in time after the anoectochilus formosanus plants are damaged by heat and before the anoectochilus formosanus plants are completely withered, so that the plants are prevented from being completely withered, the planting yield can be ensured, the loss is reduced, and the social and economic benefits are obvious.

Description

Application of cytokinin 6-BA in prevention and treatment of high-temperature heat damage of anoectochilus formosanus

Technical Field

The invention relates to the technical field of chemical agriculture, in particular to application of cytokinin 6-BA in prevention and treatment of high-temperature heat damage of anoectochilus formosanus.

Background

6-benzylamino adenine (6-BA), alternative name: 6-benzylaminopurine, cytokinin, common english name: 6-Benzylaminopurine, molecular formula: c₁₂H₁₁N₅Molecular weight: 225.26, white crystalline powder, poorly water soluble, slightly ethanol soluble, stable in acid, base, structural formula:

6-BA is widely used and the favourite cytokinin for tissue culture because of its high efficiency, stability, low cost and ease of use. The main function of 6-BA is to promote the formation of buds and also to induce callus formation. Is often used for improving the quality and the yield of tea and tobacco; the fresh keeping of vegetables and fruits and the cultivation of rootless bean sprouts obviously improve the quality of fruits and leaves. The main physiological effects are as follows: promoting cell division; promoting differentiation of non-differentiated tissues; promoting cell enlargement and fattening; promoting the germination of seeds; inducing the growth of dormant buds; inhibiting or promoting the elongation growth of stems and leaves; inhibiting or promoting root growth; inhibiting leaf aging; breaking the apical dominance and promoting the growth of lateral buds; promoting flower bud formation and flowering; inducing a female trait; promoting fruit setting; promoting the growth of fruits; inducing tuber formation; the transportation and accumulation of substances; inhibit or promote respiration; evaporation and pore opening are promoted; the anti-injury capability is improved; inhibiting the decomposition of chlorophyll; promoting or inhibiting the activity of the enzyme. However, 6-BA is not reported in the aspect of heat damage prevention and control of anoectochilus formosanus.

Anoectochilus roxburghii is also called Kalipa japonica, also called Anoectochilus roxburghii, namely Anoectochilus roxburghii, hypericum japonicum, Chinese starwort, Anoectochilus roxburghii, Anoectochilus formosanus and the like, and the Mindonnan also has a name of 'Jinbuhui' call. Mainly distributed in Taiwan, Fujian, Zhejiang, Jiangxi, Guangdong, Guangxi, Hainan, Yunnan, Sichuan, Guizhou, Tibet and other provinces of China, wherein Taiwan, Fujian, Zhejiang and Jiangxi are the main production areas. Anoectochilus roxburghii belongs to orchidaceae herbaceous plants, is a perennial precious Chinese herbal medicine, and has high medicinal value and ornamental value. Anoectochilus roxburghii grows slowly naturally, has low reproduction rate, only one in a thousand of the emergence rate of seeds, is extremely rare in natural quantity due to the damage of natural environment and artificial excessive digging, and is listed as an endangered medicinal plant by the government of Fujian province mainly producing the medicinal material in 1990. The wild anoectochilus formosanus is endangered and extincted, artificial breeding is an effective way for relieving insufficient supply of anoectochilus formosanus market, and the existing anoectochilus formosanus tissue culture technology is quite mature. However, when the tissue culture seedling of anoectochilus formosanus is planted in the field, due to the change of climatic conditions, nutritional conditions and cultivation technology, various damages which seriously affect the growth and yield of the anoectochilus formosanus exist, the common damages include diseases, insect pests and mice, and in addition, the growth of the anoectochilus formosanus is directly or indirectly threatened by high temperature, continuous rainy days and wind damages. The most suitable growth temperature range of the anoectochilus formosanus is 20-25 ℃, when the culture temperature is 25 ℃, the growth speed of the anoectochilus formosanus is fastest, the growth vigor is most vigorous, and when the culture temperature is higher than 35 ℃, the anoectochilus formosanus can die due to heat damage. Therefore, in high-temperature seasons, the anoectochilus formosanus is easily damaged by heat, and mainly shows various adverse phenomena such as withered leaf edges, curled leaves, dehydrated leaf edges, withered leaves, yellow and lusterless leaves, crispness and the like, so that the normal growth of the anoectochilus formosanus is seriously influenced, and the yield and the quality of the anoectochilus formosanus are reduced. When the temperature continues to rise to the thermal limit, the net photosynthetic rate will be zero, and if the temperature continues to rise, the leaves will be sweet due to severe water loss, even dry and dead. In the prior art, the measure for dealing with the heat damage of anoectochilus formosanus is mainly prevention, shading, cooling and ventilation, and the like, for example, shading by using a shading net to reduce light intensity and temperature, and spray cooling can be carried out by adopting a micro-sprinkling irrigation system. For plants which are already subjected to heat injury and have heat injury symptoms, in the prior art, no medicine or method capable of enabling the heat injury plants to grow and rejuvenate again appears.

Disclosure of Invention

Aiming at the defects of the prior art, the invention provides the application of cytokinin 6-BA in preventing and treating high-temperature heat damage of anoectochilus formosanus in order to effectively prevent and treat heat damage in the artificial cultivation process of the anoectochilus formosanus and improve the quality and the yield of the anoectochilus formosanus product, solves the rejuvenation problem of the anoectochilus formosanus after the heat damage occurs in the artificial cultivation process by an active pesticide application mode, and provides reference for artificial planting of the anoectochilus formosanus.

In order to achieve the purpose, the invention adopts the following technical scheme:

application of cytokinin 6-BA in preventing and treating high temperature heat injury of Anoectochilus roxburghii is provided. The application of the cytokinin 6-BA comprises the step of spraying the whole anoectochilus roxburghii plant with a 6-BA solution with the concentration of 0.1-1.0 g/L. The application of the cytokinin 6-BA comprises the step of spraying 6-BA with the concentration of 0.1-1.0 g/L once or for multiple times on anoectochilus formosanus plants which generate heat damage, wherein when the spraying is carried out for multiple times, the spraying is carried out once every 2-7 days for 2-5 times. The application of the cytokinin 6-BA comprises the step of spraying the whole anoectochilus roxburghii plants which generate heat damage with 6-BA solution with the concentration of 0.5 g/L. Application of cytokinin 6-BA in preparing a prevention and treatment medicament for high-temperature heat damage of anoectochilus formosanus.

Compared with the prior art, the invention has the following beneficial effects:

because the temperature management of the anoectochilus formosanus planting is unbalanced, the temperature of the growth environment where the anoectochilus formosanus is positioned rises suddenly and exceeds the thermal limit temperature, and leaves can be withered and necrotic due to severe dehydration. According to the invention, the Anoectochilus roxburghii plant is sprayed with the 6-BA solution, so that the Anoectochilus roxburghii plant can grow new buds and rejuvenate. The 6-BA solution disclosed by the invention can achieve a good rejuvenation effect on a plurality of anoectochilus formosanus varieties. The 6-BA solution is sprayed in time after the anoectochilus formosanus plants are damaged by heat and before the anoectochilus formosanus plants are completely withered, so that the plants are prevented from being completely withered, the planting yield can be ensured, the loss is reduced, and the social and economic benefits are obvious.

Drawings

FIG. 1 shows the growth of heat-damaged J2 plants before spraying 6-BA solution.

FIG. 2 shows the growth of heat-damaged NKS1 plants before spraying 6-BA solution.

FIG. 3 shows the growth of heat-damaged J3 plants before spraying 6-BA solution.

FIG. 4 shows the growth of the whole 6-BA solution in treatment 3 after 30 days of spraying.

FIG. 5 shows the growth of the whole 6-BA solution in treatment 5 after 30 days of spraying.

FIG. 6 shows the growth of the whole 6-BA solution in treatment 8 after 30 days of spraying.

FIG. 7 shows the growth of the whole 6-BA solution in treatment 3 after spraying for 45 days.

FIG. 8 shows the growth of the whole 6-BA solution in treatment 5 after spraying for 45 days.

FIG. 9 shows the growth of the whole 6-BA solution in treatment 8 after 45 days of spraying.

FIG. 10 shows the growth of the treated 6-BA solution in 3 after spraying the whole plant for 3 months.

FIG. 11 shows the growth of the whole plant treated with 6-BA solution in treatment 5 after spraying for 3 months.

FIG. 12 shows the growth of the treated 6-BA solution in 8 after spraying the whole plant for 3 months.

FIG. 13 shows the growth of treated 3 plants after spraying 6-BA solution for 4 months.

FIG. 14 shows the growth of treated 5 plants after spraying 6-BA solution for 4 months.

FIG. 15 shows the growth of treated 8 plants after spraying 6-BA solution for 4 months.

FIG. 16 shows the growth of the whole 6-BA solution in treatment 7 after spraying for 40 days.

FIG. 17 shows the growth of the 6-BA solution in treatment 7 after 55 days of spraying the whole plant.

FIG. 18 shows the growth of the entire 6-BA solution in treatment 10 after 3 sprays for 3 months.

Detailed Description

The technical solution of the present invention is further illustrated by the following examples.

Example 1

(1) The content is as follows: 6-BA with different concentrations is sprayed on the anoectochilus formosanus which has generated heat damage, and the growth and recovery conditions of the anoectochilus formosanus are observed.

(2) Materials:

(2.1) plant material: selecting a batch of anoectochilus formosanus plants of J2, NKS1 and J3 respectively and having the same growth condition, wherein the anoectochilus formosanus plants are subjected to stress treatment at high temperature of 40 ℃ and then have heat damage symptoms of wilting or drying terminal buds, as shown in figures 1-3; and (4) cleaning terminal buds of the anoectochilus formosanus which are subjected to heat damage, and then respectively treating according to the method in the step (3), wherein the treatment is carried out within 24 hours after the stress treatment. The heat resistance of three anoectochilus formosanus is J2, NKS1 and J3.

(2.2) reagents: putting 6-BA powder into a beaker, adding 1% dilute hydrochloric acid, stirring until the solution is dissolved, then adding water to a constant volume, and respectively preparing 6-BA solutions with 3 concentrations: 1g/L, 0.5g/L and 0.1 g/L.

(3) The treatment method comprises the following steps:

treatment 1: the variety is J2, 3 plants in total, and the whole Anoectochilus roxburghii is sprayed once by using 6-BA solution with the concentration of 0.1g/L, wherein each plant is 5 ml;

and (3) treatment 2: the variety is J2, 3 plants in total, and the whole Anoectochilus roxburghii is sprayed once by using 6-BA solution with the concentration of 0.5g/L, wherein each plant is 5 ml;

and (3) treatment: the variety is J2, 3 plants in total, and the whole Anoectochilus roxburghii is sprayed once by using 6-BA solution with the concentration of 1.0g/L, wherein each plant is 5 ml;

and (4) treatment: the variety is NKS1, 3 plants are adopted, and the whole Anoectochilus roxburghii is sprayed once by using 6-BA solution with the concentration of 0.1g/L, wherein each plant is 5 ml;

and (4) treatment 5: the variety is NKS1, 3 plants are adopted, and 6-BA solution with the concentration of 0.5g/L is used for spraying the whole anoectochilus formosanus once, wherein each plant is 5 ml;

and (6) treatment: the variety is NKS1, 3 strains are obtained in total, and the whole Anoectochilus roxburghii is sprayed once by using 6-BA solution with the concentration of 1.0g/L, wherein each strain is 5 ml;

and (7) treatment: the variety is J3, 3 plants in total, and the whole Anoectochilus roxburghii is sprayed once by using 6-BA solution with the concentration of 0.1g/L, wherein each plant is 5 ml;

and (4) treatment 8: the variety is J3, 3 plants in total, and the whole Anoectochilus roxburghii is sprayed once by using 6-BA solution with the concentration of 0.5g/L, wherein each plant is 5 ml;

and (4) treatment: the variety is J3, 3 plants in total, and the whole Anoectochilus roxburghii is sprayed once by using 6-BA solution with the concentration of 1.0g/L, wherein each plant is 5 ml;

and (4) treatment 10: the variety is J2, 3 plants in total, the heated anoectochilus formosanus is sprayed for a plurality of times, 5 ml/time, with 6-BA solution with the concentration of 0.5g/L, the next day of spraying is washed with clean water, and the spraying is carried out once every 3 days and 3 times in total.

And (3) treatment 11: the variety is NKS1, 3 strains in total, the heated anoectochilus formosanus is sprayed for a plurality of times, 5 ml/time, with 6-BA solution with the concentration of 0.5g/L, the next day of spraying is washed with clean water, and the spraying is carried out once every 3 days and 3 times in total.

And (4) treatment 12: the variety is J3, 3 plants in total, the heated anoectochilus formosanus is sprayed for a plurality of times, 5 ml/time, with 6-BA solution with the concentration of 0.5g/L, the next day of spraying is washed with clean water, and the spraying is carried out once every 3 days and 3 times in total.

And (4) treatment: the variety was J2, 3 in total, and 5ml of distilled water was sprayed once as a control.

And (4) processing: the variety was NKS1, 3 in total, and was sprayed once with 5ml of distilled water as a control.

And (4) treatment: the variety was J3, 3 in total, and 5ml of distilled water was sprayed once as a control.

The growth environmental conditions and the management method of the other plants are completely consistent, and the plants treated in the test process are all in proper growth conditions.

(4) And (3) processing results:

(4.1) after 25 days of treatment, J2 (i.e. treatment 2) sprayed once at a concentration of 0.5g/L initially shoots new axillary buds, and each of the other treated plants continued to shoot new buds within one week thereafter, indicating that the plants began to resume growth, as shown in FIGS. 4-6.

(4.2) after treatment for 40 days, complete branches and buds grow out of axillary parts of all plants, even if Anoectochilus roxburghii with serious heat damage and shrunken stems sprays for 40 days at one time, new buds also emerge from roots, and after emerging for 45 days, the new buds grow vigorously, as shown in figures 7-9.

(4.3) As shown in FIGS. 10 to 12, after 3 months of treatment, the average plant height of each treated plant was measured, the initial scale was 10cm, and the actual plant height was-10 cm, and from the results in Table 1, the results of the multiple treatments were better than those of the one-time treatment, and the one-time spray treatment was followed by the concentration of 0.5 g/L. The control group died completely after 3 months, which shows that only the 6-BA solution is sprayed, and the heat damage anoectochilus formosanus plants can grow new buds and rejuvenate.

TABLE 1 average plant height (cm) of Anoectochilus roxburghii after three months of spraying 6-BA of different concentrations

(4.4) As shown in FIGS. 13 to 15, after 4 months of treatment, the results of the low-concentration one-time treatment are more desirable in terms of the number of newly-developed lateral branches, and particularly, the results of the 0.5g/L one-time spraying are the best.

When the multi-time spraying is used for simulating outdoor planting, the 6-BA solution which is just sprayed can be washed away by raining, and the content of the 6-BA on the plant can be kept by timely supplementing the spraying, so that the plant can grow new buds and can be rejuvenated.

When 6-BA with the concentration of 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9 or 1.0g/L is used for spraying the anoectochilus formosanus plants which generate heat damage for a plurality of times, the anoectochilus formosanus plants can be sprayed once every 2, 3, 4, 5, 6 or 7 days according to the requirement of actual conditions, and the spraying is carried out for 2, 3, 4 or 5 times in total before and after, so that the heat damage plants grow new buds and are rejuvenated.

According to the test results, the agent for preventing and treating the high-temperature heat injury of the anoectochilus formosanus can be prepared by taking the cytokinin 6-BA as a main effective component and adding other auxiliary components.

Claims

1. The application of cytokinin 6-BA in preventing and treating high-temperature heat injury of anoectochilus formosanus is characterized in that 6-BA with the concentration of 0.1-1.0 g/L is used for spraying the anoectochilus formosanus plants which generate heat injury once or for multiple times, and when the 6-BA is sprayed for multiple times, the 6-BA is sprayed once every 2-7 days for 2-5 times.

2. The application of cytokinin 6-BA in preventing and treating high-temperature heat damage of anoectochilus formosanus as claimed in claim 1, wherein 6-BA solution with concentration of 0.1-1.0 g/L is used for spraying the whole anoectochilus formosanus plants which generate heat damage.

3. The use of cytokinin 6-BA in preventing and treating high-temperature heat damage of Anoectochilus roxburghii as claimed in claim 1, wherein 6-BA solution with concentration of 0.5g/L is used for spraying the whole Anoectochilus roxburghii plants which generate heat damage.