CN106386115A - Regulation and control method for improving heat resistance of dahlia - Google Patents
Regulation and control method for improving heat resistance of dahlia Download PDFInfo
- Publication number
- CN106386115A CN106386115A CN201610800494.0A CN201610800494A CN106386115A CN 106386115 A CN106386115 A CN 106386115A CN 201610800494 A CN201610800494 A CN 201610800494A CN 106386115 A CN106386115 A CN 106386115A
- Authority
- CN
- China
- Prior art keywords
- dahlia
- high temperature
- salicylic acid
- dahlia pinnata
- acid solution
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Engineering & Computer Science (AREA)
- Biodiversity & Conservation Biology (AREA)
- Ecology (AREA)
- Forests & Forestry (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention discloses a regulation and control method for improving heat resistance of dahlia. The method includes: preparing a salicylic acid solution with the concentration of 0.1-1.5 mmol/L, spraying leaves with the salicylic acid solution in 1-2 days before high temperature stress during a growth period or a flowering period of the dahlia, continuously spraying the leaves for 2 days, and spraying the leaves once in the morning and evening every day respectively; and spraying the leaves once every 6-8 days during continuous high temperature stress. After the dahlia is sprayed with the salicylic acid solution, dahlia plants grow and develop in a relative normal state, and most of the dahlia plants grow well in a high temperature stress environment; leaves of the dahlia plants are dark green, and the chlorophyll relative content and the net photosynthetic rate are increased; the number of flowers of a single plant is increased, the flower diameter is increased, and the full-bloom period is prolonged; resistant physical signs such as autioxidant enzyme activities, malondialdehyde, proline and soluble proteins of the leaves of the dahlia change correspondingly, and then tolerance of the dahlia in the high temperature environment can be effectively improved, which has positive effects on introduction, open field cultivation and landscape application of the dahlia.
Description
Technical field
The present invention relates to a kind of flowers resistance related cultivation technique is and in particular to a kind of raising Dahlia Pinnata Cav. thermostability
Regulation and control method, belongs to fancy horticulture technical field.
Background technology
Dahlia Pinnata Cav.（Dahlia pinnataCav.）For the perennial flowering bulb of Compositae dahlia, flower appearance grace, pattern
Delicate and charming, various in style, the florescence is long long, and variant kind plant height difference is big, and suitably potted plant and open country beautification, in Landscape
See configuration to be frequently used for arranging flower bed, flower border, be also suitably for potted plant, garden and plant, be improve urban ecological environment, the beautification appearance of the city and
The excellent material of family.The graceful kind of flower pattern also can carry out cut-flower production, can be used as bouquet, the decoration such as the gaily decorated basket, wall flower, bottle are inserted
Product.
Dahlia Pinnata Cav. originates in Mexican Plateau area, the warm and nice and cool weather of property happiness, and high temperature stress will have a strong impact on beautiful greatly
The growth promoter of flower, also affects its ornamental value and Landscape Application.When high 30 DEG C of temperature, plant growth arrest, bloom less, very
Extremely can not bloom.The Summer High Temperature weather of especially south China provinces and regions, can suppress Dahlia Pinnata Cav. normally to grow and bloom,
Affect its ornamental plantation to be worth.Therefore, using suitable method, effectively alleviate the shadow to Dahlia Pinnata Cav. growth promoter for the high temperature stress
Ring, Dahlia Pinnata Cav. is introduced a fine variety, cultivates and Landscape Application is all significant.
So far, about the also rarely seen report of research of Dahlia Pinnata Cav. adverse circumstance aspect, it is concentrated mainly on the water of Dahlia Pinnata Cav. at present
Divide stress and Aging Mechanism aspect.Already clear, after plant is subject to high temperature injury, growth retardation, typically heat evil occurs
Symptom, the wherein floral organ as germinal tissue are particularly sensitive to temperature.Therefore, for ornamental plant, once high temperature suppression
Flowering of plant processed, will have a strong impact on its ornamental value and economic worth.How to effectively improve the resistance of Dahlia Pinnata Cav. grass, improve
Thermostability, its regulation and control method is worth research.
Content of the invention
The deficiency that the present invention exists in terms of Dahlia Pinnata Cav. resistance for prior art, provides one kind to be applied to Dahlia Pinnata Cav. life
Long different phase, in high temperature environments, effectively improves the thermostability of Dahlia Pinnata Cav., promotes the tune of the growth promoter of Dahlia Pinnata Cav. plant
Prosecutor method.
The technical scheme realizing the object of the invention is to provide a kind of regulation and control method improving Dahlia Pinnata Cav. thermostability, including as follows
Step：
1st, judge the trophophase residing for Dahlia Pinnata Cav., described trophophase includes isolated culture stage, vegetative growth phase and florescence；
2nd, according to judged result, chemical regulation is carried out to the thermostability of Dahlia Pinnata Cav.；
Vegetative growth phase：The tissue cultured seedling of taking root that the seedling of seed propagation, isolated culture are obtained is transplanted to land for growing field crops or carries out potted plant,
When Dahlia Pinnata Cav. growth of seedling is to plant height 15～20 more than cm, preparation quality volume fraction is 0.1～1.5 mmol L-1Water
Poplar acid solution, 1～2 d before high temperature stress, salicylic acid solution is uniformly squirted to the upper and lower surface of blade, continuously sprays 2 d,
The daily morning and evening respectively sprays 1 time；Or when running into continuous high temperature stress, at interval of 5～7 d, the upper and lower surface of blade is uniformly sprayed
Wet salicylic acid solution is once；
Florescence：When Dahlia Pinnata Cav. plant strain growth to terminal bud starts bud differentiation-the budding stage, preparing quality volume fraction is
0.1～1.5 mmol L-1Salicylic acid solution, 2～4 d before high temperature stress, the blade upper and lower surface to part below alabastrum
Uniformly squirt salicylic acid solution, continuously spray 2 d, the daily morning and evening respectively sprays 1 time；Or when running into continuous high temperature stress, every
Every 5～7 d, salicylic acid solution is uniformly squirted once to the upper and lower surface of blade；
The isolated culture stage：By salicylic acid with quality volume fraction for 0.01～1.0 mmol L-1Concentration be added to culture
In base, before high temperature stress, successive transfer culture is carried out to test tube seedling.
The thermostability regulation and control in Dahlia Pinnata Cav. isolated culture stage specifically include following steps as mentioned above：
1st, with Dahlia Pinnata Cav. axillary bud as explant, culture medium MS+0.1 mg L is inoculated in using conventional disinfection method-16-BA+
0.5 mg·L-1NAA+1.0 mg·L-1GA3On, isolated culture obtains test tube seedling；
2nd, adding concentration in 1/2 MS culture medium is 0.05～1.0 mmol L-1Salicylic acid, cut a length of 1.5～2.5
The Dahlia Pinnata Cav. test tube seedling of cm carries out successive transfer culture；
3rd, successive transfer culture condition is light application time 12 h/d, and intensity of illumination is 3500～4000 lx, and temperature conditionss are 24～26
℃；
4th, after successive transfer culture 10～15d, obtain tolerating the Dahlia Pinnata Cav. test tube seedling of high temperature stress.
The environmental condition of high temperature stress of the present invention is 30～32 DEG C of 34～37 DEG C/night in daytime.
Compared with prior art, the marked improvement of the present invention is：
1st, under high temperature stress environment, the present invention when applying exogenous chemical substances, after testing, send out by plant physiology and biochemical metabolism
Give birth to adaptations, the resistance of itself has been strengthened with this.Therefore, the chemical regulation method that can be provided by the present invention, in height
Under warm environment, effectively improve the thermostability of Dahlia Pinnata Cav., promote the growth promoter of Dahlia Pinnata Cav. plant.
2nd, the method that the present invention adopts chemical regulation relevant physiological biochemical indicator, the Dahlia Pinnata Cav. plant to different growth phases
Apply salicylic acid, under can improving hot environment while growth and development state, also there occurs in plant body and be conducive to strengthening
The physiological change of thermostability.
3rd, the present invention is with Dahlia Pinnata Cav. tissue cultured seedling, trophophase Potted orchard, florescence Potted orchard as objective for implementation, using Salicylic Acid
Low-kappa number method, the thermostability of induction Dahlia Pinnata Cav., is the impact to ornamental plant for the researching high-temperature and salicylic acid is improving sight
The application aspect of reward plant heat resistance property provides new experimental basis, for instructing Dahlia Pinnata Cav. introducing and planting and Landscape Application to provide necessarily
Theoretical foundation.
Specific embodiment
Embodiment 1：
The band root tissue cultured seedling of Dahlia Pinnata Cav. kind ' single-lobe is yellow ' is transplanted to potted plant, conventional cultivation manages.When plant strain growth to 15～
20 cm height more than vegetative growth phase when, spray 0.1～1.5 mmol L-1Salicylic acid, all uniform with blade upper and lower surface
Squirt and be advisable；Daily time-division morning and evening sprays 1 time, continuously sprays 2 d, then gives high temperature stress（30 DEG C of 35 DEG C/night in daytime）Ring
Border condition.Result, in the case of the high temperature stress of artificial setting, has sprayed 0.5～1.0 mmol L-1Salicylic Dahlia Pinnata Cav.
Vine growth and development is more normal, dark green leaf color, and compared with not spraying salicylic matched group, chlorophyll relative content increased
18.2%～25.0%, net photo-synthesis rate improves 29.7%～57.21%.Meanwhile, the antioxidase of Dahlia Pinnata Cav. blade
The physiological and biochemical indexs such as activity, malonaldehyde, proline, soluble protein also all there occurs corresponding change, thus effectively improving
The toleration to High Temperature Stress for the Dahlia Pinnata Cav..
Meanwhile, to the above-mentioned plant being in vegetative growth phase, when running into continuous high temperature stress, at interval of 5～7d, right
The upper and lower surface of blade uniformly squirts 0.1～1.5 mmol L-1Salicylic acid solution once；With do not spray salicylic comparison
Group is compared, and the contrast of the toleration to hot environment for the Dahlia Pinnata Cav. is similar to the present embodiment aforementioned result.
Embodiment 2：
The band root tissue cultured seedling of Dahlia Pinnata Cav. kind ' single-lobe is yellow ' is transplanted to potted plant, conventional cultivation manages.In plant strain growth to bud
Differentiation-aobvious flower bud stage, when being in florescence, foliage-spray 0.1～1.5 mmol L-1Salicylic acid, with blade upper and lower surface all
Uniformly squirt and be advisable；Daily time-division morning and evening sprays 1 time, continuously sprays 2 d, is subsequently placed in summer natural high-temperature（Daytime 34～37
DEG C/30～32 DEG C of night）Environmental condition under carry out potted plant.Result, in the case of high temperature stress, has sprayed 0.5～1.0 mmol
L-1Salicylic Dahlia Pinnata Cav. plant shows as growth promoter normally, and the excellent plant of growing way is in the majority, has reached more than 90%；With not
Spray salicylic matched group to compare, plant dark green leaf color, chlorophyll relative content increased 19.8%～33.3%；Individual plant is bloomed
Amount increased 1.0～1.5 times, and Hua Jing increases 21.5%～35.8%, and full-bloom stage extends 2.2～2.5 d.Meanwhile, greatly
The physiological and biochemical indexs such as the activities of antioxidant enzymes of beautiful floral leaf piece, malonaldehyde, proline, soluble protein also all there occurs accordingly
Change, thus effectively increase the toleration to hot environment for the Dahlia Pinnata Cav..
Meanwhile, to the above-mentioned Dahlia Pinnata Cav. being in florescence, when running into continuous high temperature stress, at interval of 5～7d, to leaf
The upper and lower surface of piece uniformly squirts 0.1～1.5 mmol L-1Salicylic acid solution is once；With do not spray salicylic matched group phase
The contrast of the toleration to hot environment for the Dahlia Pinnata Cav. is similar to the present embodiment aforementioned result for ratio.
Embodiment 3：
With Dahlia Pinnata Cav. axillary bud as explant, culture medium MS+0.1 mg L is inoculated in using conventional disinfection method-16-BA+0.5
mg·L-1NAA+1.0 mg·L-1GA3On, isolated culture obtains test tube seedling.Adding concentration in 1/2 MS culture medium is 0.01
～1.0 mmol L-1Salicylic acid, cut be about 1.5～2.5 cm Dahlia Pinnata Cav. Plantlet subculture cultivate 10～15 d after, turn
Move on to light application time 12 h/d, intensity of illumination is 3500～4000 lx, carries out high temperature stress in the environment of 30 DEG C of 35 DEG C/night of daytime
Process.Result with the addition of 0.02～0.05 mmol L-1Cultivate in salicylic culture medium, the growth of Dahlia Pinnata Cav. test tube seedling is good
Good, induction root system is more, increased 15.4%～97.7% respectively than comparison；And, Dahlia Pinnata Cav. test tube seedling leaf under high temperature stress
Mda content reduce, proline and content of soluble protein increase, superoxide dismutase, peroxidase and peroxide
Change hydrogenase activity strengthen, that is, using add in culture medium salicylic method can induce Dahlia Pinnata Cav. improve thermostability, this is also
Salicylic acid method raising Dahlia Pinnata Cav. thermostability is applied using root and provides foundation.
Claims (3)
1. a kind of regulation and control method improving Dahlia Pinnata Cav. thermostability is it is characterised in that comprise the steps：
（1）Judge the trophophase residing for Dahlia Pinnata Cav., described trophophase includes isolated culture stage, vegetative growth phase and florescence；
（2）According to judged result, chemical regulation is carried out to the thermostability of Dahlia Pinnata Cav.；
Vegetative growth phase：The tissue cultured seedling of taking root that the seedling of seed propagation, isolated culture are obtained is transplanted to land for growing field crops or carries out potted plant,
When Dahlia Pinnata Cav. growth of seedling is to plant height 15～20 more than cm, preparation quality volume fraction is 0.1～1.5 mmol L-1Water
Poplar acid solution, 1～2 d before high temperature stress, salicylic acid solution is uniformly squirted to the upper and lower surface of blade, continuously sprays 2 d,
The daily morning and evening respectively sprays 1 time；Or when running into continuous high temperature stress, at interval of 5～7 d, the upper and lower surface of blade is uniformly sprayed
Wet salicylic acid solution is once；
Florescence：When Dahlia Pinnata Cav. plant strain growth to terminal bud starts bud differentiation-the budding stage, preparing quality volume fraction is
0.1～1.5 mmol L-1Salicylic acid solution, 2～4 d before high temperature stress, the blade upper and lower surface to part below alabastrum
Uniformly squirt salicylic acid solution, continuously spray 2 d, the daily morning and evening respectively sprays 1 time；Or when running into continuous high temperature stress, every
Every 5～7 d, salicylic acid solution is uniformly squirted once to the upper and lower surface of blade；
The isolated culture stage：By salicylic acid with quality volume fraction for 0.01～1.0 mmol L-1Concentration be added to culture medium
In, before high temperature stress, successive transfer culture is carried out to test tube seedling.
2. a kind of regulation and control method of the raising Dahlia Pinnata Cav. thermostability according to claims 1 is it is characterised in that described beautiful greatly
The thermostability regulation and control in flower isolated culture stage comprise the steps：
（1）With Dahlia Pinnata Cav. axillary bud as explant, culture medium MS+0.1 mg L is inoculated in using conventional disinfection method-16-BA+
0.5 mg·L-1NAA+1.0 mg·L-1GA3On, isolated culture obtains test tube seedling；
（2）Adding concentration in 1/2 MS culture medium is 0.05～1.0 mmol L-1Salicylic acid, cut a length of 1.5～2.5
The Dahlia Pinnata Cav. test tube seedling of cm carries out successive transfer culture；
（3）Successive transfer culture condition is light application time 12 h/d, and intensity of illumination is 3500～4000 lx, and temperature conditionss are 24～26
℃；
（4）After successive transfer culture 10～15 d, obtain tolerating the Dahlia Pinnata Cav. test tube seedling of high temperature stress.
3. a kind of raising Dahlia Pinnata Cav. thermostability according to claims 1 regulation and control method it is characterised in that：Described high temperature
The environmental condition of stress is 30～32 DEG C of 34～37 DEG C/night in daytime.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610800494.0A CN106386115A (en) | 2016-09-02 | 2016-09-02 | Regulation and control method for improving heat resistance of dahlia |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610800494.0A CN106386115A (en) | 2016-09-02 | 2016-09-02 | Regulation and control method for improving heat resistance of dahlia |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106386115A true CN106386115A (en) | 2017-02-15 |
Family
ID=57998550
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610800494.0A Pending CN106386115A (en) | 2016-09-02 | 2016-09-02 | Regulation and control method for improving heat resistance of dahlia |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106386115A (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107306632A (en) * | 2017-06-29 | 2017-11-03 | 成都苗夫现代苗木科技有限公司 | A kind of culturing and reproducing of dahlia |
| CN109041975A (en) * | 2018-09-10 | 2018-12-21 | 天津大学 | A method of alleviating triclosan using salicylic acid and plant stress is injured |
| CN110915563A (en) * | 2019-11-07 | 2020-03-27 | 广东省农业科学院作物研究所 | Application of cytokinin 6-BA in prevention and treatment of high-temperature heat damage of anoectochilus formosanus |
| CN113575180A (en) * | 2021-07-20 | 2021-11-02 | 西北农林科技大学 | Method for improving heat resistance of plants |
| CN114007417A (en) * | 2019-03-29 | 2022-02-01 | 环球化学股份有限公司 | Crop plant sun protection agent |
| CN117158424A (en) * | 2023-11-02 | 2023-12-05 | 浙江大学海南研究院 | Method for improving heat resistance of cabbage pollen by treating different agents in different periods of pollen development |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1391801A (en) * | 2001-04-25 | 2003-01-22 | 四川龙蟒福生科技有限责任公司 | Plant growth regualting composition with drought resistance function |
| CN104584889A (en) * | 2013-10-30 | 2015-05-06 | 上海市农业科学院 | Identification method for screening heat-resistant properties of different cucumber varieties under heat stress |
-
2016
- 2016-09-02 CN CN201610800494.0A patent/CN106386115A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1391801A (en) * | 2001-04-25 | 2003-01-22 | 四川龙蟒福生科技有限责任公司 | Plant growth regualting composition with drought resistance function |
| CN104584889A (en) * | 2013-10-30 | 2015-05-06 | 上海市农业科学院 | Identification method for screening heat-resistant properties of different cucumber varieties under heat stress |
Non-Patent Citations (3)
| Title |
|---|
| 向地英等: "水杨酸对菊花耐热性的影响", 《江苏农业科学》 * |
| 管华、张萍萍等: "高温胁迫及外源水杨酸对大丽花花瓣XTHs基因表达的影响", 《中国观赏园艺研究进展2016》 * |
| 赵川乐，张萍萍等: "高温胁迫及外源水杨酸对大丽花抗氧化酶活性的影响", 《中国观赏园艺研究进展2016》 * |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107306632A (en) * | 2017-06-29 | 2017-11-03 | 成都苗夫现代苗木科技有限公司 | A kind of culturing and reproducing of dahlia |
| CN109041975A (en) * | 2018-09-10 | 2018-12-21 | 天津大学 | A method of alleviating triclosan using salicylic acid and plant stress is injured |
| CN114007417A (en) * | 2019-03-29 | 2022-02-01 | 环球化学股份有限公司 | Crop plant sun protection agent |
| CN110915563A (en) * | 2019-11-07 | 2020-03-27 | 广东省农业科学院作物研究所 | Application of cytokinin 6-BA in prevention and treatment of high-temperature heat damage of anoectochilus formosanus |
| CN110915563B (en) * | 2019-11-07 | 2022-03-22 | 广东省农业科学院作物研究所 | Application of cytokinin 6-BA in prevention and treatment of high-temperature heat damage of anoectochilus formosanus |
| CN113575180A (en) * | 2021-07-20 | 2021-11-02 | 西北农林科技大学 | Method for improving heat resistance of plants |
| CN117158424A (en) * | 2023-11-02 | 2023-12-05 | 浙江大学海南研究院 | Method for improving heat resistance of cabbage pollen by treating different agents in different periods of pollen development |
| CN117158424B (en) * | 2023-11-02 | 2024-01-16 | 浙江大学海南研究院 | Method for improving heat resistance of cabbage pollen by treating different agents in different periods of pollen development |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106386115A (en) | Regulation and control method for improving heat resistance of dahlia | |
| CN104885773B (en) | A kind of method of quickly breeding blue berry early stage sizing tissue culture commercial seedling | |
| CN103461050A (en) | Loquat cultivation method for dwarfing and early fruiting | |
| CN103460971B (en) | Method for improving transplanting survival rate of trichosanthes kirilowii tissue culture seedlings | |
| CN106069143A (en) | A kind of Flos Chrysanthemi implantation methods in light type plant factor | |
| CN103609441A (en) | Blueberry seedling culturing method | |
| CN104855156A (en) | Tree shape cultivating method for Yunnan azaleas | |
| CN104705056A (en) | Strawberry planting method for promoting flowering | |
| CN104798688A (en) | Culture method for akebiaquinata decne seedlings | |
| CN105309148A (en) | Petunia hybrid planting method | |
| CN103814762B (en) | A kind of light compensation method improving tree peony annual flower cultivation quality | |
| CN106818476B (en) | The efficient quick propagation method of natural Triploid Lacquer Tree | |
| CN103069989A (en) | Method for producing esthetic bryophyte bottle | |
| CN108605643A (en) | A kind of training technique of vinifera plantation | |
| CN104904595A (en) | Anthurium adndraeanum rapid propagation method | |
| CN107493926A (en) | A kind of high yield high-quality implantation methods of watermelon | |
| CN107125137A (en) | A kind of fast breeding method of Idesia polycarpa sapling | |
| CN106332711A (en) | Method for planting green tea trees in high-altitude mountainous area | |
| CN107027461A (en) | The method of pinching quickly breeding Growth anddevelopment potted flower | |
| CN106718907A (en) | A kind of planting technology of edible lily | |
| DK202270523A1 (en) | Method for high-efficiently inducing double stems of phalaenopsis | |
| CN109076853A (en) | A kind of implantation methods for the growth cycle shortening tribute mandarin orange nursery stock | |
| CN108713464A (en) | A kind of low light tolerance tomato planting method | |
| CN101755679B (en) | Lipstick plant internode shortening technology | |
| CN104904594B (en) | A kind of red palm pollen cultures method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20170215 |