CN109041975A - A method of alleviating triclosan using salicylic acid and plant stress is injured - Google Patents
A method of alleviating triclosan using salicylic acid and plant stress is injured Download PDFInfo
- Publication number
- CN109041975A CN109041975A CN201811048727.1A CN201811048727A CN109041975A CN 109041975 A CN109041975 A CN 109041975A CN 201811048727 A CN201811048727 A CN 201811048727A CN 109041975 A CN109041975 A CN 109041975A
- Authority
- CN
- China
- Prior art keywords
- triclosan
- seed
- salicylic acid
- rinsed
- water
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/45—Tobacco
Abstract
Alleviate the method that triclosan injures plant stress using salicylic acid the invention discloses a kind of, it is characterized in that the following steps are included: (1) carries out seed sprouting: the complete and dry plant maturation seed of picking kind skin, it is 70%-80% ethanol water with volumetric concentration, it impregnates 1-3 minutes, with rinsed with sterile water 2-4 times, it is impregnated 10-20 minutes with the liquor natrii hypochloritis of the 5%-10% containing effective chlorine again, it is layed on MS culture medium with after rinsed with sterile water 2-4 times, 25 DEG C/22 DEG C round the clock, brightness 16h/8h cultivated in alternate greenhouse, sprout seed；(2) triclosan and salicylic acid handle seedling simultaneously: it is sprouted to seed, when the complete spread apart of cotyledon, selects and grow strong seedling, be transplanted in soil, pour containing triclosan and salicylic water, grow, harvest.Raw material of the present invention is easy to get, and method is simple, easily operated, and cost is relatively low.
Description
Technical field
The present invention relates to the phytoremediation fields of organic pollutant, and in particular to a kind of to alleviate triclosan pair using salicylic acid
The method of plant stress injury.
Background technique
Triclosan (TCS) is currently used wide spectrum, high-efficient antibacterial agent, to gram-positive bacteria, Gram-negative bacteria, ferment
Female, virus has efficient killing and inhibiting effect, is widely used in personal care product (such as shampoo, toothpaste, soap
Deng), daily necessities (such as woollen blanket, movement footgear), medical supplies (such as preservative, fungicide) and household cleaning articles are (such as
Dish washing liquid, disinfectant) etc., usage amount is huge.According to statistics, the year output of whole world triclosan is close to 1500 tons.
Triclosan enters foul water system with the use of nursing product and daily necessities, after sewage disposal system is handled,
Triclosan can not be completely eliminated, and still be can detect that triclosan in the water outlet of sewage treatment plant, be discharged into environment
And downstream water is polluted.There are 85 rivers in 139 rivers in 30 states in the studies have shown that U.S. of US Geological Survey
It detects containing triclosan, maximum concentration 2300ng/L；Triclosan concentration is 10-90ng/L in the lake of Switzerland；Spain
Hemipelagic sediment in residual triclosan at concentrations up to 130.7ug/kg；The content of the korneforos the Shi Jing triclosan of China's Pearl River Delta
For 345-1329ng/L, situation allows of no optimist.Remaining triclosan enters in soil through sewage irrigation in sewage, adsorbs simultaneously
Into the triclosan in activated sludge as agricultural sludge also enters in agricultural land soil.Therefore, triclosan has become earth's surface
One of highest emerging pollutant of frequency is detected in water body and soil.
Studies have shown that:, by the irradiation of ultraviolet light, photodissociation can occur for the triclosan in water body under the conditions of higher pH
Generate dioxin；Triclosan has a significant impact to the survival and reproduction of aquatile, and it is unbalance to may result in aquatic ecosystem；Three
Chlorine life can influence the biology community structure and function of soil nitrogen cycle and soil；Triclosan is doubtful carcinogenicity chlorophenols object
Matter shows endocrine to mammal.
At present about in the Research on Ecological Effect of triclosan, being more to evaluate its influence to aquatile, to land
The research of biological influence is less in the ecosystem.
Summary of the invention
Alleviate triclosan to plant using salicylic acid it is an object of the invention to overcome the deficiencies of the prior art and provide a kind of
The method of stress damage.
Technical solution of the present invention is summarized as follows:
A method of alleviating triclosan using salicylic acid and plant stress injured, comprising the following steps:
(1) carry out seed sprouting: the complete and dry plant maturation seed of picking kind skin, is 70%- with volumetric concentration
80% ethanol water impregnates 1-3 minutes, molten with rinsed with sterile water 2-4 times, then with the sodium hypochlorite of the 5%-10% containing effective chlorine
Liquid impregnates 10-20 minutes, with being layed on MS culture medium after rinsed with sterile water 2-4 times, 25 DEG C/22 DEG C round the clock, brightness 16h/
8h is cultivated in alternate greenhouse, sprouts seed；
(2) triclosan and salicylic acid handle seedling simultaneously:
It is sprouted to seed, when the complete spread apart of cotyledon, selects and grow strong seedling, be transplanted in soil, it is dense to pour triclosan
Degree be 50-150mg/L, the water that Determination of Salicylic Acid is 1-2mmol/L, growth, harvest.
Preferred plant is wild-type tobacco.
Advantages of the present invention:
(1) raw material of the present invention is easy to get, and method is simple, easily operated, and cost is relatively low, has a good application prospect.
(3) there is significant application value to the phytoremediation aspect of research stress resistance of plant aspect and organic pollutant.
Detailed description of the invention
Fig. 1 is that salicylic acid is influenced to by triclosan stress tobacco strain.
Fig. 2 is tobacco chlorophyll content in varied situations.
Fig. 3 is tobacco mda content in varied situations.
In figure, WT is 1 wild-type tobacco of reference examples；WT+TCS is reference examples 2；WT+TCS+SA is embodiment 1.
Specific embodiment
The present invention by taking wild-type tobacco as an example, be in order to enable those skilled in the art to more fully understand the present invention,
But the present invention is not imposed any restrictions.
Wild-type tobacco seed is commercially available.
TCS reagent (triclosan) is purchased from Tianjin Ding Guo biotech firm, purity 98%, and it is 1mg/mL that when use, which is configured to concentration,
Mother liquor, methanol hydrotropy, using preceding through 0.2um membrane filtration to germ-free condition.
SA reagent (salicylic acid) is purchased from Tianjin Ding Guo biotech firm, purity 98%, and it is 1mol/L that when use, which is configured to concentration,
Mother liquor, ethyl alcohol dissolution, using preceding through 0.2um membrane filtration to germ-free condition.
MS culture medium (MS culture medium (MS Medium) 4.74g/L, sucrose (sucrose) 30g/L, agar powder (Agar)
7g/L, pH=5.8),
The present invention is further illustrated combined with specific embodiments below.
Embodiment 1
A method of alleviating triclosan using salicylic acid and plant stress injured, comprising the following steps:
(1) carry out seed sprouting: the complete and dry wild-type tobacco mature seed of picking kind skin is with volumetric concentration
75% ethanol water impregnates 2 minutes, impregnates 15 with rinsed with sterile water 3 times, then with the liquor natrii hypochloritis containing effective chlorine 5%
Minute, with being layed on MS culture medium after rinsed with sterile water 3 times, 25 DEG C/22 DEG C round the clock, in the alternate greenhouse brightness 16h/8h
Culture sprouts wild-type tobacco seed；
(2) triclosan and salicylic acid handle seedling simultaneously:
It is sprouted to wild-type tobacco seed, when the complete spread apart of cotyledon, selects and grow strong seedling, be transplanted in soil,
The water that triclosan concentration is 100mg/L, Determination of Salicylic Acid is 1.5mmol/L is poured, is grown, harvest.
(seedling in Fig. 1 is 7 days photographs of plantation)
At wild type tobacco seedling growth regulation 7 days, chlorophyll content, mda content are measured.
Reference examples 1:
Step (1) is the same as 1 step of embodiment (1)；
(2) it is sprouted to wild-type tobacco seed, when the complete spread apart of cotyledon, selects and grow strong seedling, be transplanted to soil
In, it waters, grows, harvest.(seedling in Fig. 1 is 7 days photographs of plantation)
At wild type tobacco seedling growth regulation 7 days, chlorophyll content, mda content are measured.
Reference examples 2:
Step (1) is the same as 1 step of embodiment (1)；
(2) it is sprouted to wild-type tobacco seed, when the complete spread apart of cotyledon, selects and grow strong seedling, be transplanted to soil
In,
The water that triclosan concentration is 100mg/L is poured, is grown, harvest.(seedling in Fig. 1 is 7 days photographs of plantation)
At wild type tobacco seedling growth regulation 7 days, chlorophyll content, mda content are measured.
Experimental result:
(1) 1 wild-type tobacco seedling of reference examples grows fine.Compared with reference examples 1, in reference examples 2 growth of tobacco seedling by
Inhibiting to obvious, strain is short and small, and tobacco leaf is narrow and obvious jaundice, the number that blade is sprouted also significantly reduce, and main root is carefully short,
The length and quantity of lateral root have apparent reduction, show that triclosan has overt toxicity to tobacco seedling.1 wild-type tobacco of embodiment
Seedling is substantially reduced by triclosan toxic action.Compared with wild-type tobacco seedling in reference examples 2, strain is obvious high larger, and
The quantity that yellowing leaf effect mitigates and blade is sprouted increases.Illustrate low concentration salicylic acid to the poison by triclosan stress tobacco strain
Evil has relaxation effect.
(2) from figure 2 it can be seen that after being coerced by triclosan, the intracorporal chlorophyll content of 2 tobacco plant of reference examples
It is remarkably decreased, illustrates that triclosan can generate damage to Chloroplast Ultrastructure.For embodiment 1 compared with reference examples 2, plant is intracorporal
Chlorophyll content obviously increases, and illustrates that salicylic acid can alleviate the damage that triclosan generates tobacco chloroplast.
(3) from figure 3, it can be seen that after being coerced by triclosan, the intracorporal mda content of 2 tobacco plant of reference examples
It dramatically increases, is to illustrate that triclosan exacerbates cigarette in reference examples 1 3.5 times of mda content in tobacco plant body in reference examples 2
The extent of peroxidation of careless cell adipose membrane.It is 2.4 times of the intracorporal mda content of tobacco plant in reference examples 1 in embodiment 1, says
Bright salicylic acid, which has, alleviates the damage that triclosan generates tobacco cell adipose membrane.
Embodiment 2
A method of alleviating triclosan using salicylic acid and plant stress injured, comprising the following steps:
(1) carry out seed sprouting: the complete and dry wild-type tobacco mature seed of picking kind skin is with volumetric concentration
70% ethanol water impregnates 3 minutes, impregnates 20 with rinsed with sterile water 2 times, then with the liquor natrii hypochloritis containing effective chlorine 5%
Minute, with being layed on MS culture medium after rinsed with sterile water 2 times, 25 DEG C/22 DEG C round the clock, in the alternate greenhouse brightness 16h/8h
Culture sprouts wild-type tobacco seed；
(2) triclosan and salicylic acid handle seedling simultaneously:
It is sprouted to wild-type tobacco seed, when the complete spread apart of cotyledon, selects and grow strong seedling, be transplanted in soil,
The water that triclosan concentration is 50mg/L, Determination of Salicylic Acid is 1mmol/L is poured, is grown, harvest.
At wild type tobacco seedling growth regulation 7 days, tobacco seedling growing way was similar to Example 1；
It is similar to Example 1 to measure chlorophyll content, mda content.
Embodiment 3
A method of alleviating triclosan using salicylic acid and plant stress injured, comprising the following steps:
(1) carry out seed sprouting: the complete and dry wild-type tobacco mature seed of picking kind skin is with volumetric concentration
80% ethanol water impregnates 1 minute, impregnates 10 with rinsed with sterile water 4 times, then with the liquor natrii hypochloritis containing effective chlorine 10%
Minute, with being layed on MS culture medium after rinsed with sterile water 4 times, 25 DEG C/22 DEG C round the clock, in the alternate greenhouse brightness 16h/8h
Culture sprouts wild-type tobacco seed；
(2) triclosan and salicylic acid handle seedling simultaneously:
It is sprouted to wild-type tobacco seed, when the complete spread apart of cotyledon, selects and grow strong seedling, be transplanted in soil,
The water that triclosan concentration is 150mg/L, Determination of Salicylic Acid is 2mmol/L is poured, is grown, harvest.
At wild type tobacco seedling growth regulation 7 days, tobacco seedling growing way was similar to Example 1；
It is similar to Example 1 to measure chlorophyll content, mda content.
Claims (2)
1. a kind of alleviate the method that triclosan injures plant stress using salicylic acid, it is characterized in that the following steps are included:
(1) carry out seed sprouting: the complete and dry plant maturation seed of picking kind skin, is 70%-80% second with volumetric concentration
Alcohol solution impregnates 1-3 minutes, impregnates with rinsed with sterile water 2-4 times, then with the liquor natrii hypochloritis of the 5%-10% containing effective chlorine
10-20 minutes, with being layed on MS culture medium after rinsed with sterile water 2-4 times, 25 DEG C/22 DEG C round the clock, brightness 16h/8h alternating
Greenhouse in cultivate, sprout seed；
(2) triclosan and salicylic acid handle seedling simultaneously: it is sprouted to seed, when the complete spread apart of cotyledon, selects and grow strong seedling,
It is transplanted in soil, pours the water that triclosan concentration is 50-150mg/L, Determination of Salicylic Acid is 1-2mmol/L, grow, harvest.
2. according to the method described in claim 1, it is characterized in that the plant is wild-type tobacco.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811048727.1A CN109041975A (en) | 2018-09-10 | 2018-09-10 | A method of alleviating triclosan using salicylic acid and plant stress is injured |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811048727.1A CN109041975A (en) | 2018-09-10 | 2018-09-10 | A method of alleviating triclosan using salicylic acid and plant stress is injured |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109041975A true CN109041975A (en) | 2018-12-21 |
Family
ID=64760050
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811048727.1A Pending CN109041975A (en) | 2018-09-10 | 2018-09-10 | A method of alleviating triclosan using salicylic acid and plant stress is injured |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109041975A (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110338000A (en) * | 2019-07-16 | 2019-10-18 | 天津大学 | A method of alleviating nano zine oxide jointly using fungi microorganism Aspergillus aculeatus and salicylic acid and plant stress is injured |
| CN110352964A (en) * | 2019-07-09 | 2019-10-22 | 天津大学 | Brassinosteroid and salicylic composition improve triclosan and coerce the lower photosynthetic application of tobacco |
| CN110352963A (en) * | 2019-07-09 | 2019-10-22 | 天津大学 | The application of brassinosteroid and salicylic composition and alleviation triclosan to tobacco oxidative stress |
| CN110367260A (en) * | 2019-07-09 | 2019-10-25 | 天津大学 | Brassinosteroid improves triclosan and coerces the lower photosynthetic purposes of tobacco |
| CN110419535A (en) * | 2019-07-09 | 2019-11-08 | 天津大学 | Brassinosteroid alleviates triclosan to the purposes of tobacco oxidative stress |
| CN110438149A (en) * | 2019-08-08 | 2019-11-12 | 天津大学 | Turn the purposes of the resistance to triclosan of LcSABP genetic tobacco |
Citations (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1970179A (en) * | 2005-11-22 | 2007-05-30 | 天津市环境保护科学研究院 | Pentachlorophenol polluted soil united directional restoration method using 'plant-microorganism' |
| CN101548673A (en) * | 2009-05-12 | 2009-10-07 | 湖北省烟草科研所 | Tobacco alkalinity reducing agent used in field tobacco cultivation |
| CN102986391A (en) * | 2011-09-08 | 2013-03-27 | 郑州大学 | Method for improving content and aromas of tobacco secondary metabolite products |
| CN103240267A (en) * | 2013-04-28 | 2013-08-14 | 华中师范大学 | Soil restoration method based on oxygen activation of iron powder and polyphosphoric acid molecules |
| CN103801556A (en) * | 2014-02-18 | 2014-05-21 | 广州中国科学院先进技术研究所 | Method for repairing soil containing novel organic pollutant by using sludge biological carbon |
| CN104126460A (en) * | 2014-08-22 | 2014-11-05 | 中国烟草总公司重庆市公司奉节分公司 | Method for preventing and controlling bacterial leaf spots of tobaccos |
| CN104860369A (en) * | 2015-04-22 | 2015-08-26 | 苏州大学 | Method for adsorption recovery of triclosan in sewage by using penicillium |
| CN105689371A (en) * | 2015-12-13 | 2016-06-22 | 深圳多元拓展环保科技有限公司 | In-situ capturing treatment method for soil heavy metal pollution |
| CN106111061A (en) * | 2016-07-29 | 2016-11-16 | 四川水利职业技术学院 | Carrier of triclosan and preparation method thereof in sewage is efficiently removed under wide pH value |
| CN106190162A (en) * | 2016-08-11 | 2016-12-07 | 李红玉 | A kind of ecological garden green soil renovation agent |
| CN106386115A (en) * | 2016-09-02 | 2017-02-15 | 苏州大学 | Regulation and control method for improving heat resistance of dahlia |
| CN106818326A (en) * | 2016-12-26 | 2017-06-13 | 新昌县钧国生物技术有限公司 | A kind of method for improving tobacco bacterial wilt resistance |
| CN106889106A (en) * | 2017-01-24 | 2017-06-27 | 新疆天业（集团）有限公司 | A kind of plant growth regulator for strengthening paddy drought resistance |
| CN107517709A (en) * | 2017-09-14 | 2017-12-29 | 河南农业大学 | It is a kind of to prevent high temperature from forcing ripe planting method of tobacco |
| CN108212217A (en) * | 2018-01-18 | 2018-06-29 | 南京大学 | A kind of catalyst of degrading chlorophenol pollutant, preparation method and application |
| CN108277173A (en) * | 2018-01-12 | 2018-07-13 | 西北农林科技大学 | The double of plant resistance to environment stress connect bacterium method in a kind of raising heavy-metal contaminated soil |
-
2018
- 2018-09-10 CN CN201811048727.1A patent/CN109041975A/en active Pending
Patent Citations (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1970179A (en) * | 2005-11-22 | 2007-05-30 | 天津市环境保护科学研究院 | Pentachlorophenol polluted soil united directional restoration method using 'plant-microorganism' |
| CN101548673A (en) * | 2009-05-12 | 2009-10-07 | 湖北省烟草科研所 | Tobacco alkalinity reducing agent used in field tobacco cultivation |
| CN102986391A (en) * | 2011-09-08 | 2013-03-27 | 郑州大学 | Method for improving content and aromas of tobacco secondary metabolite products |
| CN103240267A (en) * | 2013-04-28 | 2013-08-14 | 华中师范大学 | Soil restoration method based on oxygen activation of iron powder and polyphosphoric acid molecules |
| CN103801556A (en) * | 2014-02-18 | 2014-05-21 | 广州中国科学院先进技术研究所 | Method for repairing soil containing novel organic pollutant by using sludge biological carbon |
| CN104126460A (en) * | 2014-08-22 | 2014-11-05 | 中国烟草总公司重庆市公司奉节分公司 | Method for preventing and controlling bacterial leaf spots of tobaccos |
| CN104860369A (en) * | 2015-04-22 | 2015-08-26 | 苏州大学 | Method for adsorption recovery of triclosan in sewage by using penicillium |
| CN105689371A (en) * | 2015-12-13 | 2016-06-22 | 深圳多元拓展环保科技有限公司 | In-situ capturing treatment method for soil heavy metal pollution |
| CN106111061A (en) * | 2016-07-29 | 2016-11-16 | 四川水利职业技术学院 | Carrier of triclosan and preparation method thereof in sewage is efficiently removed under wide pH value |
| CN106190162A (en) * | 2016-08-11 | 2016-12-07 | 李红玉 | A kind of ecological garden green soil renovation agent |
| CN106386115A (en) * | 2016-09-02 | 2017-02-15 | 苏州大学 | Regulation and control method for improving heat resistance of dahlia |
| CN106818326A (en) * | 2016-12-26 | 2017-06-13 | 新昌县钧国生物技术有限公司 | A kind of method for improving tobacco bacterial wilt resistance |
| CN106889106A (en) * | 2017-01-24 | 2017-06-27 | 新疆天业（集团）有限公司 | A kind of plant growth regulator for strengthening paddy drought resistance |
| CN107517709A (en) * | 2017-09-14 | 2017-12-29 | 河南农业大学 | It is a kind of to prevent high temperature from forcing ripe planting method of tobacco |
| CN108277173A (en) * | 2018-01-12 | 2018-07-13 | 西北农林科技大学 | The double of plant resistance to environment stress connect bacterium method in a kind of raising heavy-metal contaminated soil |
| CN108212217A (en) * | 2018-01-18 | 2018-06-29 | 南京大学 | A kind of catalyst of degrading chlorophenol pollutant, preparation method and application |
Non-Patent Citations (1)
| Title |
|---|
| 陈东等: "三氯生对蛋白核小球藻的毒性效应分析", 《浙江树人大学学报(自然科学版)》 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110352964A (en) * | 2019-07-09 | 2019-10-22 | 天津大学 | Brassinosteroid and salicylic composition improve triclosan and coerce the lower photosynthetic application of tobacco |
| CN110352963A (en) * | 2019-07-09 | 2019-10-22 | 天津大学 | The application of brassinosteroid and salicylic composition and alleviation triclosan to tobacco oxidative stress |
| CN110367260A (en) * | 2019-07-09 | 2019-10-25 | 天津大学 | Brassinosteroid improves triclosan and coerces the lower photosynthetic purposes of tobacco |
| CN110419535A (en) * | 2019-07-09 | 2019-11-08 | 天津大学 | Brassinosteroid alleviates triclosan to the purposes of tobacco oxidative stress |
| CN110338000A (en) * | 2019-07-16 | 2019-10-18 | 天津大学 | A method of alleviating nano zine oxide jointly using fungi microorganism Aspergillus aculeatus and salicylic acid and plant stress is injured |
| CN110438149A (en) * | 2019-08-08 | 2019-11-12 | 天津大学 | Turn the purposes of the resistance to triclosan of LcSABP genetic tobacco |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN109041975A (en) | A method of alleviating triclosan using salicylic acid and plant stress is injured | |
| Terry et al. | Rates of selenium volatilization among crop species | |
| Gibson et al. | Barley straw as an inhibitor of algal growth II: laboratory studies | |
| Zheng et al. | Cyanobacteria can allelopathically inhibit submerged macrophytes: effects of Microcystis aeruginosa extracts and exudates on Potamogeton malaianus | |
| Crawford et al. | Tolerance of anoxia and the metabolism of ethanol in tree roots | |
| Zhou et al. | Reproduction capacity of Potamogeton crispus fragments and its role in water purification and algae inhibition in eutrophic lakes | |
| CN107787973B (en) | Application of rhamnolipid in relieving plant salt stress | |
| CN105961205B (en) | A kind of method for tissue culture for improving pearl guava survival rate | |
| CN113754204A (en) | Aquaculture pollution prevention and control and water resource recycling system and operation method | |
| Fu et al. | Controlling the main source of green tides in the Yellow Sea through the method of biological competition | |
| CN107164262A (en) | A kind of microbial bacterial agent and its bacterial manure available for controlling crop diseases and insect pests | |
| CN110447413B (en) | Watermelon fusarium oxysporum physiological race 2 resistance induction method | |
| Raymont et al. | Studies on the mass culture of Phaeodactylum | |
| CN112889849B (en) | Composite plant extracts, compositions and methods of use for controlling the growth of cladophora | |
| CN110338000A (en) | A method of alleviating nano zine oxide jointly using fungi microorganism Aspergillus aculeatus and salicylic acid and plant stress is injured | |
| CN106614810A (en) | Potamogeton malaianus-sodium alginate pellet-type algistat as well as preparation method and application thereof | |
| CN215799069U (en) | Aquaculture pollution prevention and control and water resource recycling system | |
| KR20180097494A (en) | Cosmetic ingcluding Adhesive Diatoms extract and manufacutring method thereof | |
| CN114620837A (en) | Method for rebuilding micro-saline water lake composite ecological restoration system by taking watermifoil as dominant species | |
| CN106577649A (en) | Method for alleviating panax notoginseng self-poisoning injuries by using oxalic acid | |
| CN102273490A (en) | Algae inhibitor, and preparation method and application thereof | |
| CN105028494A (en) | Chromolaena odorata-source water hyacinth growth regulating agent and preparation method | |
| CN109820003A (en) | A kind of terrestrial plant source algae-inhibiting agent and preparation method thereof | |
| KR100928013B1 (en) | Method for improvement in growth of Echinochola crusgalli using Belamcanda chinensis extract and method for remediation of heavy-metal contaminated soil using the method | |
| Chromiński | Effect of plant growth regulators on membrane permeability of tomato leaf cells |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20181221 |
|
| WD01 | Invention patent application deemed withdrawn after publication |